--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -647.82 -660.96 2 -647.93 -665.33 -------------------------------------- TOTAL -647.87 -664.65 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.094619 0.000665 0.050248 0.148044 0.091328 1421.47 1441.77 1.000 r(A<->C){all} 0.105840 0.003778 0.008375 0.228855 0.094510 316.81 425.09 1.000 r(A<->G){all} 0.172186 0.006908 0.032141 0.335241 0.159813 318.51 361.90 1.000 r(A<->T){all} 0.080640 0.002776 0.001671 0.178999 0.071481 327.85 469.68 1.001 r(C<->G){all} 0.039367 0.001549 0.000008 0.119285 0.027322 349.85 497.00 1.001 r(C<->T){all} 0.505276 0.010267 0.310899 0.698400 0.505749 446.42 470.40 1.000 r(G<->T){all} 0.096691 0.003135 0.005646 0.203415 0.087039 462.70 479.18 1.002 pi(A){all} 0.241701 0.000468 0.199043 0.283782 0.241689 1119.63 1226.01 1.000 pi(C){all} 0.250569 0.000503 0.208812 0.296062 0.249816 1173.45 1223.01 1.001 pi(G){all} 0.192929 0.000398 0.155918 0.233177 0.192453 1288.05 1294.35 1.000 pi(T){all} 0.314801 0.000550 0.271634 0.361426 0.313900 1199.69 1217.12 1.000 alpha{1,2} 0.600158 0.564324 0.000015 2.072402 0.337430 1044.67 1169.53 1.000 alpha{3} 1.197001 1.047704 0.000705 3.243417 0.934710 925.73 1004.67 1.000 pinvar{all} 0.572390 0.037415 0.169863 0.876821 0.605757 745.32 758.27 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Tue Oct 25 21:14:53 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=9, Len=121 C1 ------------------MMRVQRPPTLLLLLLVANAFSKPIGTPIPEHC C2 ------------------MMRVQRPPTLLLILLVANAFSKPIGTSIPEHC C3 MMSMRSRRSMFIEHFNELMMRVQRPPTLFLILLVANAFSKPIGTPIPEHC C4 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC C5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC C6 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC C7 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC C8 -------------------MRVQRPPTLLLILLVANAFSKPIGTPIPEHC C9 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC *********:*:*************.:**** C1 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C2 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C3 STLAGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C4 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C6 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C7 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C8 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C9 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT ***.********************************************** C1 SYDTSDPQLLSDIGYSFDYGK C2 SYDTSDPQLLSDIGYSFDYGK C3 SYDTSDPQQLSDIGYSFDYGK C4 SYDTSDPQLLSDIGYSFDYGK C5 SYDTSDPQLLSDIGYSFDYGK C6 SYDTSDPQLLSDIGYSFDYGK C7 SYDTSDPQLLSDIGYSFDYGK C8 SYDNSDPQSLSDIGYSFDYGK C9 SYDTSDPQLLSDIGYSFDYGK ***.**** ************ -- Starting log on Tue Oct 25 21:15:30 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=999, Nseq=9, Len=121 C1 ------------------MMRVQRPPTLLLLLLVANAFSKPIGTPIPEHC C2 ------------------MMRVQRPPTLLLILLVANAFSKPIGTSIPEHC C3 MMSMRSRRSMFIEHFNELMMRVQRPPTLFLILLVANAFSKPIGTPIPEHC C4 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC C5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC C6 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC C7 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC C8 -------------------MRVQRPPTLLLILLVANAFSKPIGTPIPEHC C9 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC *********:*:*************.:**** C1 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C2 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C3 STLAGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C4 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C6 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C7 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C8 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C9 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT ***.********************************************** C1 SYDTSDPQLLSDIGYSFDYGK C2 SYDTSDPQLLSDIGYSFDYGK C3 SYDTSDPQQLSDIGYSFDYGK C4 SYDTSDPQLLSDIGYSFDYGK C5 SYDTSDPQLLSDIGYSFDYGK C6 SYDTSDPQLLSDIGYSFDYGK C7 SYDTSDPQLLSDIGYSFDYGK C8 SYDNSDPQSLSDIGYSFDYGK C9 SYDTSDPQLLSDIGYSFDYGK ***.**** ************ -- Starting log on Tue Oct 25 21:14:53 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=9, Len=121 C1 ------------------MMRVQRPPTLLLLLLVANAFSKPIGTPIPEHC C2 ------------------MMRVQRPPTLLLILLVANAFSKPIGTSIPEHC C3 MMSMRSRRSMFIEHFNELMMRVQRPPTLFLILLVANAFSKPIGTPIPEHC C4 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC C5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC C6 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC C7 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC C8 -------------------MRVQRPPTLLLILLVANAFSKPIGTPIPEHC C9 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC *********:*:*************.:**** C1 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C2 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C3 STLAGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C4 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C6 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C7 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C8 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT C9 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT ***.********************************************** C1 SYDTSDPQLLSDIGYSFDYGK C2 SYDTSDPQLLSDIGYSFDYGK C3 SYDTSDPQQLSDIGYSFDYGK C4 SYDTSDPQLLSDIGYSFDYGK C5 SYDTSDPQLLSDIGYSFDYGK C6 SYDTSDPQLLSDIGYSFDYGK C7 SYDTSDPQLLSDIGYSFDYGK C8 SYDNSDPQSLSDIGYSFDYGK C9 SYDTSDPQLLSDIGYSFDYGK ***.**** ************ -- Starting log on Tue Oct 25 21:27:27 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/gapped_alignment/fubar,TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 9 taxa and 363 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C2 Taxon 3 -> C3 Taxon 4 -> C4 Taxon 5 -> C5 Taxon 6 -> C6 Taxon 7 -> C7 Taxon 8 -> C8 Taxon 9 -> C9 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1666733252 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 1026626449 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 4389161250 Seed = 357394729 Swapseed = 1666733252 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 15 unique site patterns Division 2 has 12 unique site patterns Division 3 has 20 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -736.532104 -- 32.479477 Chain 2 -- -741.079683 -- 32.479477 Chain 3 -- -739.823135 -- 32.479477 Chain 4 -- -742.255956 -- 32.479477 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -737.265244 -- 32.479477 Chain 2 -- -736.859926 -- 32.479477 Chain 3 -- -733.225374 -- 32.479477 Chain 4 -- -740.527374 -- 32.479477 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-736.532] (-741.080) (-739.823) (-742.256) * [-737.265] (-736.860) (-733.225) (-740.527) 1000 -- (-671.536) [-663.299] (-662.306) (-658.424) * (-660.936) (-665.262) [-654.433] (-658.861) -- 0:00:00 2000 -- (-663.555) (-659.442) (-659.635) [-655.398] * (-662.800) (-656.753) [-659.575] (-660.897) -- 0:00:00 3000 -- (-662.286) (-661.901) [-653.758] (-655.445) * [-654.070] (-665.058) (-658.290) (-659.325) -- 0:00:00 4000 -- (-656.173) (-654.846) [-652.782] (-657.655) * [-658.626] (-660.912) (-657.697) (-661.166) -- 0:04:09 5000 -- (-655.090) [-659.659] (-662.958) (-663.891) * (-658.066) (-663.477) [-659.555] (-655.841) -- 0:03:19 Average standard deviation of split frequencies: 0.096027 6000 -- (-656.130) (-651.422) [-651.752] (-655.666) * [-655.892] (-658.434) (-656.673) (-658.180) -- 0:02:45 7000 -- (-657.313) (-650.930) [-652.898] (-662.437) * [-652.193] (-663.218) (-661.956) (-659.464) -- 0:02:21 8000 -- (-649.524) [-657.092] (-657.084) (-665.116) * (-653.270) (-665.701) [-651.488] (-654.580) -- 0:02:04 9000 -- [-652.445] (-651.980) (-660.140) (-661.188) * (-660.803) (-657.257) (-659.499) [-654.153] -- 0:03:40 10000 -- (-666.940) [-649.893] (-663.992) (-654.799) * (-673.497) (-661.969) (-656.959) [-656.572] -- 0:03:18 Average standard deviation of split frequencies: 0.065529 11000 -- (-657.916) (-660.760) (-653.478) [-656.161] * (-653.804) [-652.161] (-674.974) (-660.275) -- 0:02:59 12000 -- (-657.211) (-656.219) (-678.836) [-649.405] * [-650.834] (-652.976) (-657.982) (-661.033) -- 0:02:44 13000 -- [-653.729] (-660.927) (-671.314) (-655.145) * (-655.062) (-653.894) (-662.960) [-654.928] -- 0:02:31 14000 -- (-654.089) (-656.326) [-648.033] (-654.098) * (-649.614) [-652.580] (-659.510) (-649.899) -- 0:03:31 15000 -- (-659.450) (-659.746) (-652.177) [-648.205] * [-652.141] (-656.546) (-662.035) (-654.086) -- 0:03:17 Average standard deviation of split frequencies: 0.066961 16000 -- (-657.337) (-660.598) [-656.005] (-650.770) * (-655.286) (-654.677) (-651.784) [-652.370] -- 0:03:04 17000 -- (-666.364) [-651.223] (-653.257) (-658.029) * (-667.917) (-659.687) [-656.538] (-659.614) -- 0:02:53 18000 -- (-654.672) [-657.266] (-653.050) (-647.528) * (-651.917) [-650.719] (-653.000) (-650.651) -- 0:03:38 19000 -- (-656.925) (-655.781) [-654.123] (-657.506) * (-656.319) (-655.869) (-657.470) [-651.812] -- 0:03:26 20000 -- (-653.306) (-668.253) [-649.953] (-657.168) * (-656.713) (-645.902) (-666.137) [-652.956] -- 0:03:16 Average standard deviation of split frequencies: 0.052463 21000 -- (-658.258) (-659.601) [-649.774] (-656.153) * (-661.230) [-657.709] (-656.517) (-658.944) -- 0:03:06 22000 -- (-653.148) (-653.690) [-649.055] (-657.654) * (-667.547) (-660.643) (-650.254) [-652.098] -- 0:03:42 23000 -- (-654.883) (-647.625) (-655.794) [-647.613] * (-648.775) (-657.392) [-649.835] (-658.863) -- 0:03:32 24000 -- (-658.316) [-650.830] (-663.821) (-652.603) * (-663.982) [-655.478] (-653.220) (-673.619) -- 0:03:23 25000 -- (-659.014) (-651.940) (-653.893) [-655.817] * (-653.541) (-651.863) [-656.503] (-658.821) -- 0:03:15 Average standard deviation of split frequencies: 0.035438 26000 -- (-656.941) (-650.130) (-653.679) [-649.797] * (-653.343) (-661.643) (-651.825) [-653.972] -- 0:03:07 27000 -- [-649.771] (-653.834) (-654.737) (-647.072) * (-662.337) (-650.650) (-653.568) [-648.490] -- 0:03:36 28000 -- [-658.139] (-656.084) (-656.372) (-652.232) * (-652.468) (-657.087) (-658.432) [-658.968] -- 0:03:28 29000 -- (-652.807) (-651.636) (-650.701) [-648.521] * [-652.586] (-653.783) (-651.282) (-648.922) -- 0:03:20 30000 -- [-654.723] (-658.981) (-652.985) (-654.069) * (-658.499) (-651.871) [-647.266] (-655.055) -- 0:03:14 Average standard deviation of split frequencies: 0.038064 31000 -- (-647.320) (-659.634) (-651.634) [-651.882] * (-655.471) (-651.589) [-652.415] (-654.523) -- 0:03:38 32000 -- [-650.134] (-657.130) (-647.520) (-665.463) * (-660.982) [-657.387] (-652.593) (-659.681) -- 0:03:31 33000 -- (-651.224) [-651.895] (-657.067) (-653.176) * (-652.742) [-656.163] (-662.925) (-654.991) -- 0:03:25 34000 -- [-646.383] (-660.057) (-651.609) (-650.110) * (-666.559) (-655.299) (-660.606) [-654.573] -- 0:03:18 35000 -- (-653.022) (-649.905) (-650.048) [-649.609] * (-662.907) (-659.606) (-659.334) [-656.354] -- 0:03:13 Average standard deviation of split frequencies: 0.028946 36000 -- (-651.466) (-658.716) (-653.316) [-650.518] * (-664.023) [-654.466] (-657.000) (-655.984) -- 0:03:34 37000 -- [-649.561] (-651.873) (-657.292) (-652.422) * (-661.220) (-647.087) [-648.514] (-662.825) -- 0:03:28 38000 -- (-657.505) (-648.795) (-653.688) [-648.865] * (-664.449) (-653.080) (-654.823) [-650.089] -- 0:03:22 39000 -- (-651.963) [-654.627] (-650.085) (-663.500) * (-659.913) (-649.879) [-651.119] (-653.682) -- 0:03:17 40000 -- (-653.210) (-656.861) [-649.427] (-654.054) * [-652.434] (-651.318) (-651.724) (-662.979) -- 0:03:36 Average standard deviation of split frequencies: 0.028980 41000 -- (-649.379) (-667.595) [-645.748] (-654.947) * (-651.837) (-655.842) (-649.460) [-656.170] -- 0:03:30 42000 -- (-651.253) (-651.670) (-660.927) [-658.753] * (-658.774) [-651.908] (-644.227) (-659.215) -- 0:03:25 43000 -- (-658.566) (-651.247) [-648.133] (-651.343) * (-646.008) (-657.647) [-648.999] (-661.547) -- 0:03:20 44000 -- (-654.820) (-658.993) [-654.378] (-655.555) * (-659.613) (-651.601) [-665.852] (-652.890) -- 0:03:15 45000 -- (-645.630) [-650.185] (-650.641) (-660.290) * (-646.732) [-647.717] (-653.790) (-655.630) -- 0:03:32 Average standard deviation of split frequencies: 0.025350 46000 -- [-648.506] (-656.302) (-663.818) (-652.713) * [-645.969] (-650.313) (-654.852) (-664.081) -- 0:03:27 47000 -- (-655.327) (-660.245) [-650.876] (-654.316) * (-656.936) (-660.239) (-652.909) [-652.910] -- 0:03:22 48000 -- [-645.006] (-657.220) (-663.051) (-655.183) * (-652.374) [-648.062] (-658.152) (-653.402) -- 0:03:18 49000 -- (-655.782) (-658.701) (-656.630) [-652.399] * (-662.121) (-663.127) [-653.565] (-652.085) -- 0:03:33 50000 -- [-652.082] (-654.289) (-662.310) (-651.860) * (-650.588) (-660.720) [-648.719] (-657.058) -- 0:03:29 Average standard deviation of split frequencies: 0.031830 51000 -- [-652.388] (-663.784) (-656.486) (-657.377) * [-661.290] (-655.133) (-649.046) (-655.391) -- 0:03:24 52000 -- (-649.776) [-655.270] (-658.664) (-653.783) * (-661.108) (-663.079) [-656.762] (-651.634) -- 0:03:20 53000 -- (-650.571) (-649.974) (-656.259) [-650.891] * (-655.287) (-655.335) [-651.529] (-655.266) -- 0:03:16 54000 -- [-649.899] (-656.586) (-647.191) (-653.857) * (-659.716) (-652.689) (-659.801) [-651.012] -- 0:03:30 55000 -- [-650.311] (-656.800) (-656.066) (-662.755) * (-658.944) (-654.809) (-654.512) [-653.856] -- 0:03:26 Average standard deviation of split frequencies: 0.030866 56000 -- (-652.365) [-657.943] (-657.308) (-657.213) * [-649.665] (-654.664) (-663.284) (-653.148) -- 0:03:22 57000 -- (-652.403) (-661.497) [-653.387] (-665.540) * (-657.823) [-655.419] (-657.046) (-648.822) -- 0:03:18 58000 -- (-651.418) [-658.766] (-653.750) (-659.667) * (-656.367) [-648.258] (-658.485) (-652.152) -- 0:03:31 59000 -- [-648.768] (-657.656) (-652.867) (-661.852) * [-654.072] (-659.605) (-666.371) (-658.995) -- 0:03:27 60000 -- (-654.076) (-653.337) [-651.285] (-664.810) * [-651.801] (-650.655) (-660.798) (-654.573) -- 0:03:23 Average standard deviation of split frequencies: 0.033996 61000 -- (-655.164) [-652.995] (-658.516) (-654.623) * [-653.630] (-652.051) (-660.169) (-647.403) -- 0:03:20 62000 -- (-650.969) (-646.925) (-654.878) [-655.596] * (-664.046) (-659.652) (-650.845) [-653.358] -- 0:03:16 63000 -- (-656.398) (-661.514) (-655.797) [-651.450] * (-654.668) [-654.555] (-663.238) (-654.660) -- 0:03:28 64000 -- (-650.679) [-652.202] (-650.504) (-659.718) * (-654.117) (-650.321) (-677.797) [-652.199] -- 0:03:24 65000 -- (-664.990) (-657.779) [-648.217] (-650.683) * (-660.388) [-647.655] (-657.000) (-648.138) -- 0:03:21 Average standard deviation of split frequencies: 0.030554 66000 -- (-654.890) (-651.459) (-654.703) [-650.017] * (-654.784) [-650.481] (-662.295) (-650.064) -- 0:03:18 67000 -- (-653.170) (-653.727) (-651.649) [-653.582] * (-655.007) (-664.600) (-653.150) [-650.797] -- 0:03:14 68000 -- [-654.834] (-654.877) (-664.846) (-656.903) * [-656.704] (-656.706) (-655.036) (-651.478) -- 0:03:25 69000 -- (-654.913) (-667.095) [-645.579] (-650.778) * (-661.517) (-654.718) [-650.635] (-653.869) -- 0:03:22 70000 -- [-651.595] (-661.174) (-652.299) (-649.825) * [-644.264] (-648.608) (-668.398) (-651.278) -- 0:03:19 Average standard deviation of split frequencies: 0.029430 71000 -- (-655.013) (-668.154) [-652.932] (-654.802) * (-660.713) (-650.912) (-662.143) [-655.542] -- 0:03:16 72000 -- [-656.687] (-656.534) (-664.675) (-654.454) * (-654.921) (-657.549) (-655.984) [-652.217] -- 0:03:26 73000 -- (-654.945) (-653.368) [-650.477] (-653.942) * [-652.216] (-653.653) (-654.015) (-648.433) -- 0:03:23 74000 -- (-655.221) (-657.558) (-665.413) [-653.087] * (-650.926) (-653.968) (-657.507) [-648.854] -- 0:03:20 75000 -- (-654.245) (-659.231) [-647.698] (-668.290) * [-645.149] (-648.635) (-652.190) (-655.460) -- 0:03:17 Average standard deviation of split frequencies: 0.028459 76000 -- (-652.835) (-658.761) (-662.819) [-651.665] * [-651.016] (-655.904) (-664.532) (-653.209) -- 0:03:14 77000 -- (-650.894) (-665.294) (-653.735) [-652.193] * (-653.637) (-661.650) (-650.495) [-656.631] -- 0:03:23 78000 -- (-657.078) (-661.982) [-651.690] (-652.303) * (-655.024) (-649.950) (-648.498) [-652.567] -- 0:03:20 79000 -- (-657.178) (-654.640) [-651.657] (-652.408) * (-648.006) (-652.763) (-652.930) [-651.166] -- 0:03:18 80000 -- (-660.193) (-648.972) (-655.359) [-654.647] * [-650.684] (-660.914) (-650.941) (-649.507) -- 0:03:15 Average standard deviation of split frequencies: 0.029609 81000 -- (-655.533) [-658.718] (-655.445) (-650.508) * (-658.776) (-659.333) [-649.504] (-663.315) -- 0:03:24 82000 -- [-652.989] (-651.178) (-649.892) (-650.980) * [-654.183] (-655.539) (-659.788) (-649.135) -- 0:03:21 83000 -- (-654.993) (-648.982) (-651.905) [-651.297] * (-647.508) (-654.910) (-656.113) [-653.800] -- 0:03:18 84000 -- (-652.133) (-667.625) (-650.795) [-649.645] * [-650.470] (-653.212) (-648.951) (-659.550) -- 0:03:16 85000 -- [-650.181] (-651.357) (-652.892) (-648.431) * (-647.903) (-655.580) (-663.326) [-650.146] -- 0:03:13 Average standard deviation of split frequencies: 0.027984 86000 -- (-649.548) (-654.581) (-649.548) [-647.781] * [-651.455] (-649.188) (-648.842) (-657.574) -- 0:03:21 87000 -- (-652.428) [-650.329] (-648.239) (-654.271) * [-652.533] (-652.696) (-663.096) (-656.624) -- 0:03:19 88000 -- (-653.155) (-658.463) (-671.452) [-652.853] * (-653.416) [-646.478] (-679.311) (-655.841) -- 0:03:16 89000 -- [-652.263] (-650.318) (-661.406) (-657.071) * [-657.499] (-656.336) (-650.623) (-652.163) -- 0:03:14 90000 -- (-660.995) [-651.536] (-659.187) (-657.541) * (-655.520) (-646.971) [-646.960] (-652.884) -- 0:03:22 Average standard deviation of split frequencies: 0.028137 91000 -- (-656.811) (-653.473) [-647.432] (-657.046) * [-659.555] (-659.370) (-652.297) (-654.534) -- 0:03:19 92000 -- [-655.221] (-655.665) (-654.983) (-653.476) * (-655.350) (-649.551) (-659.678) [-655.025] -- 0:03:17 93000 -- (-655.137) (-655.891) [-655.535] (-652.457) * (-657.355) (-646.012) (-651.695) [-649.800] -- 0:03:15 94000 -- [-657.703] (-653.893) (-650.151) (-651.539) * (-658.196) (-656.575) (-655.619) [-648.752] -- 0:03:12 95000 -- (-652.542) (-671.557) (-653.777) [-658.524] * (-645.242) (-654.294) (-655.451) [-650.914] -- 0:03:20 Average standard deviation of split frequencies: 0.026863 96000 -- (-659.958) (-653.136) (-649.383) [-652.038] * (-654.728) [-651.011] (-655.741) (-651.523) -- 0:03:17 97000 -- (-653.610) [-649.474] (-647.204) (-654.316) * [-651.815] (-656.174) (-649.915) (-653.940) -- 0:03:15 98000 -- (-665.820) (-656.056) (-647.716) [-660.406] * (-655.686) (-662.010) [-647.432] (-649.346) -- 0:03:13 99000 -- (-656.085) (-658.322) (-652.809) [-652.500] * [-651.034] (-652.736) (-652.498) (-658.083) -- 0:03:20 100000 -- [-659.235] (-659.871) (-650.050) (-654.999) * (-664.176) (-650.670) (-649.170) [-654.721] -- 0:03:18 Average standard deviation of split frequencies: 0.028617 101000 -- [-658.357] (-660.043) (-647.149) (-651.542) * [-655.331] (-649.585) (-648.560) (-662.755) -- 0:03:15 102000 -- [-651.964] (-656.523) (-652.005) (-656.256) * [-652.251] (-650.955) (-651.668) (-651.780) -- 0:03:13 103000 -- (-651.284) [-657.803] (-657.179) (-660.739) * (-659.240) (-649.558) [-650.772] (-650.511) -- 0:03:20 104000 -- (-655.785) (-653.975) (-653.298) [-649.896] * (-653.936) (-662.101) (-654.373) [-648.702] -- 0:03:18 105000 -- (-656.095) (-660.401) [-648.678] (-651.583) * (-658.584) (-652.701) [-646.803] (-653.944) -- 0:03:16 Average standard deviation of split frequencies: 0.026436 106000 -- (-653.548) (-660.167) [-651.128] (-653.441) * (-654.111) [-650.645] (-652.662) (-654.194) -- 0:03:13 107000 -- (-650.749) (-649.993) [-651.647] (-661.493) * (-656.103) (-651.536) (-656.962) [-654.869] -- 0:03:11 108000 -- (-658.885) (-646.844) [-654.282] (-658.934) * (-654.101) (-650.443) (-650.144) [-648.950] -- 0:03:18 109000 -- (-652.875) [-649.523] (-660.768) (-647.292) * [-649.337] (-662.070) (-654.082) (-650.785) -- 0:03:16 110000 -- [-654.786] (-656.880) (-655.250) (-660.846) * (-655.036) (-656.873) (-660.922) [-652.598] -- 0:03:14 Average standard deviation of split frequencies: 0.024138 111000 -- (-657.903) [-649.593] (-650.925) (-665.089) * (-656.934) [-657.241] (-651.844) (-662.776) -- 0:03:12 112000 -- (-656.187) (-651.634) [-646.532] (-652.646) * (-656.846) (-649.003) [-651.021] (-651.455) -- 0:03:18 113000 -- (-664.416) (-659.891) [-649.395] (-651.569) * (-653.478) (-655.522) [-652.039] (-655.142) -- 0:03:16 114000 -- (-661.022) [-649.178] (-664.084) (-658.061) * (-651.681) [-646.945] (-666.445) (-657.593) -- 0:03:14 115000 -- (-659.120) (-650.080) (-654.203) [-654.976] * [-652.980] (-654.980) (-652.943) (-664.752) -- 0:03:12 Average standard deviation of split frequencies: 0.024835 116000 -- (-661.103) (-652.759) [-651.521] (-652.409) * (-658.966) [-645.082] (-650.689) (-651.514) -- 0:03:18 117000 -- (-653.110) (-647.249) (-653.991) [-649.577] * [-653.721] (-651.901) (-656.750) (-650.245) -- 0:03:16 118000 -- (-649.621) (-648.945) (-654.869) [-651.756] * (-655.785) (-661.444) [-647.941] (-656.750) -- 0:03:14 119000 -- (-650.903) (-648.156) (-655.934) [-651.120] * [-656.142] (-651.961) (-656.672) (-654.595) -- 0:03:12 120000 -- (-653.045) [-648.609] (-655.462) (-651.874) * [-660.679] (-653.866) (-658.182) (-651.556) -- 0:03:10 Average standard deviation of split frequencies: 0.023440 121000 -- (-664.203) [-652.730] (-651.887) (-656.887) * [-655.749] (-649.626) (-647.143) (-658.185) -- 0:03:16 122000 -- (-651.812) [-649.047] (-654.377) (-652.056) * (-657.096) [-649.786] (-650.657) (-666.173) -- 0:03:14 123000 -- (-656.347) (-656.132) [-654.228] (-654.883) * (-654.459) (-657.620) (-650.034) [-651.228] -- 0:03:12 124000 -- (-656.193) (-650.476) [-655.880] (-658.753) * (-652.896) (-649.523) [-654.613] (-649.783) -- 0:03:10 125000 -- [-650.875] (-653.792) (-654.009) (-658.883) * (-650.663) (-658.477) [-650.157] (-651.300) -- 0:03:16 Average standard deviation of split frequencies: 0.023071 126000 -- (-654.918) [-654.770] (-657.784) (-650.707) * [-651.381] (-652.556) (-652.035) (-659.656) -- 0:03:14 127000 -- (-663.599) (-648.911) (-654.250) [-653.044] * [-648.568] (-654.931) (-654.632) (-653.855) -- 0:03:12 128000 -- (-661.176) [-652.518] (-653.001) (-659.790) * (-659.488) [-660.848] (-655.463) (-654.313) -- 0:03:10 129000 -- (-648.453) [-653.777] (-653.160) (-654.446) * (-652.056) [-649.332] (-655.912) (-659.621) -- 0:03:09 130000 -- (-659.107) (-651.264) (-660.090) [-649.672] * (-652.812) (-655.597) (-658.265) [-658.580] -- 0:03:14 Average standard deviation of split frequencies: 0.023768 131000 -- (-656.619) [-656.891] (-658.893) (-649.182) * (-648.815) (-648.183) [-650.195] (-659.823) -- 0:03:12 132000 -- (-655.147) [-656.621] (-665.288) (-665.207) * (-658.233) (-649.675) [-656.494] (-654.606) -- 0:03:10 133000 -- (-653.928) (-656.538) [-649.426] (-653.604) * [-651.511] (-660.110) (-655.362) (-659.962) -- 0:03:09 134000 -- [-654.024] (-657.785) (-664.194) (-652.655) * (-653.181) [-654.884] (-667.811) (-660.290) -- 0:03:13 135000 -- (-650.608) (-654.880) [-648.878] (-663.818) * (-660.041) (-661.126) [-648.901] (-652.314) -- 0:03:12 Average standard deviation of split frequencies: 0.021817 136000 -- (-650.270) [-652.609] (-660.680) (-653.588) * (-655.791) (-651.049) [-652.076] (-651.732) -- 0:03:10 137000 -- (-653.211) (-664.889) (-651.474) [-650.172] * (-653.893) (-650.453) (-660.925) [-652.389] -- 0:03:08 138000 -- (-654.038) (-649.623) (-655.843) [-657.409] * (-655.211) (-651.706) (-670.172) [-661.121] -- 0:03:13 139000 -- (-656.844) (-666.956) [-658.961] (-653.109) * (-655.692) [-653.989] (-667.068) (-653.342) -- 0:03:12 140000 -- (-653.997) (-654.999) (-646.717) [-645.645] * (-659.452) [-649.387] (-649.723) (-658.291) -- 0:03:10 Average standard deviation of split frequencies: 0.019910 141000 -- [-647.340] (-652.863) (-651.083) (-646.291) * (-650.878) [-649.910] (-652.708) (-664.457) -- 0:03:08 142000 -- (-654.230) [-652.773] (-664.207) (-652.882) * [-653.823] (-662.566) (-662.634) (-657.570) -- 0:03:07 143000 -- [-652.401] (-651.704) (-661.687) (-655.414) * (-653.566) [-653.914] (-652.986) (-656.199) -- 0:03:11 144000 -- (-652.984) [-653.719] (-653.353) (-657.926) * (-653.130) (-652.765) [-645.570] (-655.256) -- 0:03:10 145000 -- (-659.213) (-660.325) (-664.740) [-647.284] * (-649.105) (-663.800) [-651.713] (-655.008) -- 0:03:08 Average standard deviation of split frequencies: 0.020322 146000 -- (-650.594) (-654.631) (-670.538) [-649.126] * (-656.025) (-661.213) (-656.128) [-654.340] -- 0:03:07 147000 -- (-651.204) (-657.200) (-653.404) [-647.546] * (-654.851) (-657.651) [-650.927] (-647.244) -- 0:03:11 148000 -- (-655.200) (-651.569) [-651.074] (-651.420) * (-670.163) (-662.059) [-651.416] (-646.685) -- 0:03:09 149000 -- (-664.041) [-660.972] (-653.290) (-661.535) * [-649.606] (-655.544) (-658.465) (-655.310) -- 0:03:08 150000 -- [-655.352] (-651.481) (-655.431) (-652.187) * (-647.944) (-658.768) (-658.486) [-653.624] -- 0:03:07 Average standard deviation of split frequencies: 0.019509 151000 -- (-658.606) (-661.146) (-656.389) [-653.468] * (-649.099) (-653.960) (-659.170) [-654.092] -- 0:03:11 152000 -- (-655.349) (-658.415) [-651.606] (-652.698) * (-653.574) [-656.040] (-651.953) (-649.050) -- 0:03:09 153000 -- (-654.974) [-658.262] (-660.140) (-646.873) * (-654.363) [-663.362] (-658.128) (-664.419) -- 0:03:08 154000 -- (-657.218) (-655.410) (-656.166) [-653.098] * (-649.996) (-649.885) [-651.125] (-650.565) -- 0:03:06 155000 -- (-655.552) [-653.525] (-661.671) (-651.949) * (-650.444) [-652.691] (-656.247) (-655.355) -- 0:03:10 Average standard deviation of split frequencies: 0.019138 156000 -- (-653.477) [-650.079] (-653.014) (-648.041) * [-651.707] (-653.782) (-656.195) (-654.549) -- 0:03:09 157000 -- (-650.978) [-650.006] (-656.687) (-652.162) * (-655.287) (-653.211) (-653.051) [-649.801] -- 0:03:07 158000 -- (-650.241) [-649.827] (-660.749) (-654.189) * (-667.804) [-654.153] (-654.939) (-652.744) -- 0:03:06 159000 -- (-656.274) (-657.313) [-655.559] (-650.672) * (-651.411) [-654.837] (-657.093) (-652.338) -- 0:03:05 160000 -- [-649.982] (-656.940) (-649.629) (-661.010) * (-658.883) (-653.456) [-648.575] (-649.004) -- 0:03:09 Average standard deviation of split frequencies: 0.019330 161000 -- [-655.627] (-647.040) (-652.234) (-654.340) * (-650.004) (-657.896) [-651.733] (-646.586) -- 0:03:07 162000 -- [-652.643] (-656.211) (-663.238) (-657.956) * [-654.364] (-657.509) (-653.560) (-652.128) -- 0:03:06 163000 -- [-649.429] (-654.921) (-649.043) (-656.861) * (-649.316) (-653.150) [-656.172] (-660.830) -- 0:03:04 164000 -- [-651.008] (-650.923) (-653.872) (-651.816) * (-651.696) [-647.719] (-655.051) (-664.392) -- 0:03:08 165000 -- (-651.159) [-653.349] (-657.587) (-648.660) * (-656.056) (-655.089) (-650.482) [-648.245] -- 0:03:07 Average standard deviation of split frequencies: 0.017828 166000 -- (-651.207) (-650.093) (-651.553) [-658.372] * (-650.848) (-657.081) [-649.174] (-648.578) -- 0:03:05 167000 -- (-657.667) (-652.021) (-655.534) [-656.167] * (-663.658) (-664.482) [-649.996] (-652.840) -- 0:03:04 168000 -- [-649.285] (-655.963) (-651.875) (-648.101) * (-659.797) [-651.876] (-664.504) (-649.553) -- 0:03:03 169000 -- (-664.529) (-651.854) [-651.078] (-647.739) * [-653.771] (-650.499) (-658.262) (-649.564) -- 0:03:06 170000 -- (-656.110) (-656.830) [-660.128] (-647.691) * [-649.104] (-662.377) (-652.457) (-650.733) -- 0:03:05 Average standard deviation of split frequencies: 0.016898 171000 -- (-653.356) (-660.828) (-653.926) [-657.478] * (-655.315) (-651.121) (-645.425) [-651.176] -- 0:03:04 172000 -- (-653.939) (-654.703) [-650.426] (-655.226) * (-664.966) (-656.304) (-659.909) [-657.658] -- 0:03:02 173000 -- (-658.776) (-661.125) (-656.680) [-653.948] * [-649.931] (-652.336) (-654.256) (-657.089) -- 0:03:06 174000 -- (-653.646) (-653.393) [-657.512] (-656.478) * (-660.300) (-658.451) [-655.924] (-656.138) -- 0:03:05 175000 -- (-649.362) [-654.482] (-665.291) (-650.501) * (-648.421) (-654.346) [-653.317] (-652.032) -- 0:03:03 Average standard deviation of split frequencies: 0.017961 176000 -- (-660.325) (-652.541) [-650.444] (-654.438) * [-654.254] (-663.142) (-658.277) (-653.601) -- 0:03:02 177000 -- (-650.467) (-659.388) [-653.517] (-655.641) * (-655.639) (-654.196) (-652.727) [-650.132] -- 0:03:01 178000 -- (-655.730) (-657.480) (-657.205) [-649.013] * (-658.115) (-650.736) [-655.773] (-657.412) -- 0:03:04 179000 -- [-646.642] (-652.116) (-650.471) (-653.412) * (-650.451) (-657.078) [-653.940] (-652.484) -- 0:03:03 180000 -- [-660.121] (-647.035) (-661.594) (-655.858) * (-653.451) (-654.669) [-652.642] (-657.220) -- 0:03:02 Average standard deviation of split frequencies: 0.018091 181000 -- (-657.482) [-648.598] (-658.454) (-658.672) * (-650.076) (-657.795) (-666.831) [-650.235] -- 0:03:00 182000 -- (-651.586) (-657.828) (-661.118) [-651.744] * [-647.559] (-654.369) (-656.771) (-651.867) -- 0:03:04 183000 -- (-651.127) [-654.548] (-652.861) (-655.013) * [-657.693] (-650.643) (-655.861) (-662.226) -- 0:03:03 184000 -- (-654.271) (-661.128) (-653.465) [-651.331] * (-657.876) (-656.487) (-652.669) [-653.249] -- 0:03:01 185000 -- (-656.238) (-663.399) [-655.085] (-652.658) * (-652.128) (-652.713) [-654.550] (-662.373) -- 0:03:00 Average standard deviation of split frequencies: 0.018337 186000 -- [-653.408] (-650.313) (-659.292) (-657.318) * [-660.294] (-658.588) (-656.331) (-653.392) -- 0:03:03 187000 -- [-648.164] (-659.093) (-650.207) (-656.727) * [-648.437] (-659.732) (-657.046) (-662.942) -- 0:03:02 188000 -- [-648.606] (-658.196) (-652.747) (-655.787) * (-654.094) [-651.716] (-650.827) (-649.475) -- 0:03:01 189000 -- (-652.186) [-651.963] (-652.076) (-652.586) * (-649.297) (-662.145) (-655.956) [-659.892] -- 0:03:00 190000 -- (-656.967) (-651.547) (-654.293) [-652.086] * (-661.338) (-661.656) (-654.316) [-651.911] -- 0:03:03 Average standard deviation of split frequencies: 0.017889 191000 -- (-661.106) (-656.010) [-651.467] (-651.813) * [-652.096] (-656.374) (-654.183) (-650.814) -- 0:03:02 192000 -- (-654.338) [-653.615] (-660.071) (-653.250) * [-645.111] (-656.316) (-653.107) (-657.196) -- 0:03:00 193000 -- (-662.980) (-657.487) [-646.525] (-648.025) * (-651.584) (-658.797) [-661.211] (-656.459) -- 0:02:59 194000 -- (-653.031) (-654.863) (-647.480) [-656.728] * [-650.158] (-648.626) (-664.591) (-657.319) -- 0:02:58 195000 -- (-658.871) [-653.032] (-653.323) (-651.720) * (-656.025) (-654.018) (-655.363) [-652.215] -- 0:03:01 Average standard deviation of split frequencies: 0.017968 196000 -- (-650.815) (-654.557) [-650.759] (-650.242) * (-647.537) (-653.406) (-654.511) [-650.471] -- 0:03:00 197000 -- (-655.536) [-655.588] (-650.491) (-650.253) * (-653.577) (-654.648) (-663.106) [-653.917] -- 0:02:59 198000 -- (-655.096) (-658.807) [-652.985] (-653.383) * (-652.270) (-657.648) [-651.256] (-655.864) -- 0:02:58 199000 -- (-658.137) (-655.402) (-653.033) [-652.962] * (-649.600) (-651.147) (-669.447) [-649.868] -- 0:03:01 200000 -- (-661.711) [-653.914] (-655.414) (-657.027) * (-660.505) (-660.555) [-650.414] (-658.043) -- 0:03:00 Average standard deviation of split frequencies: 0.015818 201000 -- (-661.037) [-654.246] (-657.264) (-655.343) * [-649.728] (-659.577) (-665.288) (-660.614) -- 0:02:58 202000 -- (-655.621) [-649.493] (-652.572) (-648.355) * (-654.767) (-653.539) (-654.187) [-660.384] -- 0:02:57 203000 -- (-663.160) (-651.828) [-648.320] (-653.456) * (-649.103) (-652.631) (-656.088) [-652.738] -- 0:02:56 204000 -- (-663.724) (-646.980) (-656.823) [-654.357] * (-658.109) (-653.062) [-650.255] (-661.545) -- 0:02:59 205000 -- (-659.061) (-651.969) [-656.113] (-651.800) * (-654.526) [-655.647] (-652.325) (-663.943) -- 0:02:58 Average standard deviation of split frequencies: 0.016448 206000 -- (-653.982) [-652.847] (-658.142) (-650.992) * (-656.258) [-651.514] (-660.704) (-660.674) -- 0:02:57 207000 -- (-654.712) (-654.912) (-650.583) [-647.903] * (-645.068) [-648.760] (-650.967) (-650.866) -- 0:02:56 208000 -- (-654.254) (-653.586) [-649.893] (-655.070) * (-645.920) (-655.850) (-654.123) [-650.106] -- 0:02:58 209000 -- (-656.121) (-657.225) (-650.212) [-653.615] * (-656.145) [-646.913] (-654.433) (-653.629) -- 0:02:57 210000 -- [-657.814] (-660.198) (-649.797) (-657.779) * (-659.645) [-648.223] (-652.787) (-671.614) -- 0:02:56 Average standard deviation of split frequencies: 0.019876 211000 -- [-653.782] (-655.780) (-653.661) (-659.864) * (-651.156) [-650.190] (-657.329) (-653.788) -- 0:02:55 212000 -- (-655.387) [-654.805] (-652.033) (-652.259) * (-655.428) (-664.067) [-654.462] (-652.893) -- 0:02:54 213000 -- (-655.820) [-652.367] (-652.825) (-656.403) * (-652.526) [-646.401] (-668.677) (-658.625) -- 0:02:57 214000 -- (-654.053) (-653.680) [-649.335] (-655.223) * (-655.567) [-649.611] (-663.502) (-654.604) -- 0:02:56 215000 -- (-652.068) (-652.849) [-648.647] (-648.755) * (-660.174) (-649.320) [-652.777] (-657.804) -- 0:02:55 Average standard deviation of split frequencies: 0.019505 216000 -- (-651.487) [-652.731] (-660.747) (-649.632) * (-646.757) [-645.547] (-647.904) (-658.234) -- 0:02:54 217000 -- (-658.509) [-651.702] (-651.739) (-645.491) * (-653.334) (-652.908) (-656.088) [-652.892] -- 0:02:56 218000 -- (-658.181) (-649.150) (-654.433) [-649.645] * (-650.111) [-648.462] (-660.161) (-651.216) -- 0:02:55 219000 -- (-666.169) [-652.645] (-652.764) (-653.132) * (-656.208) [-647.660] (-651.868) (-653.457) -- 0:02:54 220000 -- (-656.040) [-649.690] (-656.226) (-656.359) * (-650.411) (-656.213) [-651.209] (-651.953) -- 0:02:53 Average standard deviation of split frequencies: 0.020028 221000 -- (-661.722) [-654.356] (-651.591) (-650.674) * (-662.163) (-654.114) (-660.317) [-647.489] -- 0:02:52 222000 -- (-664.148) (-659.266) (-656.052) [-647.964] * (-663.273) (-650.975) (-659.297) [-660.950] -- 0:02:55 223000 -- (-660.635) (-652.012) (-654.620) [-651.266] * (-658.338) (-662.029) [-659.548] (-662.586) -- 0:02:54 224000 -- (-658.419) (-651.959) [-656.073] (-654.477) * (-649.145) (-653.636) (-657.033) [-650.343] -- 0:02:53 225000 -- (-662.916) (-657.831) [-649.898] (-662.187) * (-660.035) (-652.066) [-653.943] (-661.898) -- 0:02:52 Average standard deviation of split frequencies: 0.019294 226000 -- (-656.791) (-658.363) (-655.787) [-657.652] * [-648.156] (-668.270) (-653.221) (-651.492) -- 0:02:54 227000 -- (-659.363) [-654.134] (-659.311) (-651.988) * (-652.961) (-647.541) (-652.936) [-647.398] -- 0:02:53 228000 -- [-652.913] (-646.453) (-650.963) (-659.432) * (-652.962) (-651.147) [-653.390] (-658.744) -- 0:02:52 229000 -- (-653.547) [-646.046] (-654.386) (-655.444) * (-651.747) [-653.610] (-657.462) (-655.862) -- 0:02:51 230000 -- [-655.580] (-652.139) (-656.161) (-661.407) * [-651.753] (-652.530) (-662.236) (-658.549) -- 0:02:54 Average standard deviation of split frequencies: 0.019159 231000 -- [-649.420] (-660.944) (-651.557) (-651.818) * (-655.043) [-656.121] (-660.508) (-654.100) -- 0:02:53 232000 -- (-652.828) [-658.525] (-658.558) (-655.050) * (-657.753) [-652.988] (-647.888) (-657.704) -- 0:02:52 233000 -- (-655.513) [-650.965] (-647.511) (-648.754) * [-654.056] (-661.737) (-656.327) (-654.952) -- 0:02:51 234000 -- [-643.983] (-648.350) (-653.602) (-654.649) * (-659.095) (-666.270) [-660.938] (-653.577) -- 0:02:53 235000 -- (-655.254) (-655.970) (-648.595) [-656.453] * [-658.411] (-660.490) (-654.880) (-652.578) -- 0:02:52 Average standard deviation of split frequencies: 0.018726 236000 -- (-655.938) [-649.661] (-648.747) (-656.567) * [-649.116] (-657.314) (-657.698) (-654.534) -- 0:02:51 237000 -- (-655.914) (-662.962) [-665.443] (-654.015) * (-652.615) (-654.897) [-655.411] (-650.778) -- 0:02:50 238000 -- (-668.496) (-651.452) [-655.119] (-656.586) * [-647.569] (-649.825) (-666.089) (-653.776) -- 0:02:49 239000 -- (-658.634) (-655.588) [-656.638] (-656.567) * [-649.856] (-650.156) (-653.344) (-664.148) -- 0:02:51 240000 -- (-651.841) (-655.392) (-652.639) [-651.978] * (-653.860) [-650.214] (-660.665) (-674.368) -- 0:02:51 Average standard deviation of split frequencies: 0.018543 241000 -- (-647.353) [-656.314] (-662.048) (-652.983) * [-647.270] (-653.718) (-648.647) (-656.313) -- 0:02:50 242000 -- (-650.553) (-656.886) [-658.677] (-664.035) * (-652.742) (-650.077) [-650.358] (-654.371) -- 0:02:49 243000 -- (-651.773) (-661.938) [-650.942] (-654.715) * [-655.354] (-650.314) (-653.454) (-657.078) -- 0:02:51 244000 -- (-656.721) [-657.109] (-658.580) (-652.018) * [-648.890] (-650.325) (-652.416) (-654.442) -- 0:02:50 245000 -- (-659.362) (-658.726) (-647.727) [-652.097] * [-650.421] (-654.293) (-647.202) (-653.431) -- 0:02:49 Average standard deviation of split frequencies: 0.019546 246000 -- (-649.118) (-660.835) [-651.082] (-653.028) * (-646.285) (-650.689) (-650.000) [-658.259] -- 0:02:48 247000 -- (-650.060) (-657.243) (-657.010) [-653.170] * (-651.672) (-650.444) [-648.511] (-651.653) -- 0:02:50 248000 -- [-649.942] (-655.487) (-659.356) (-652.985) * (-650.671) (-653.287) (-662.903) [-647.582] -- 0:02:49 249000 -- [-647.008] (-651.697) (-652.266) (-654.977) * (-654.165) [-653.433] (-652.410) (-652.135) -- 0:02:48 250000 -- (-652.418) (-654.452) (-650.596) [-647.266] * (-659.560) [-645.815] (-656.644) (-649.299) -- 0:02:48 Average standard deviation of split frequencies: 0.018336 251000 -- [-657.311] (-649.877) (-660.061) (-647.759) * (-660.290) [-649.492] (-659.874) (-657.005) -- 0:02:50 252000 -- (-650.380) (-649.310) [-652.526] (-651.537) * (-659.338) [-650.306] (-660.980) (-649.897) -- 0:02:49 253000 -- (-648.650) (-659.239) [-650.276] (-649.252) * [-650.960] (-663.283) (-659.075) (-653.253) -- 0:02:48 254000 -- [-653.349] (-653.535) (-661.865) (-650.292) * [-648.829] (-649.697) (-662.385) (-654.726) -- 0:02:47 255000 -- (-659.474) [-649.397] (-655.296) (-657.416) * (-650.095) [-649.089] (-657.767) (-655.363) -- 0:02:46 Average standard deviation of split frequencies: 0.018660 256000 -- (-653.746) (-654.258) (-648.567) [-656.122] * [-651.696] (-659.346) (-650.699) (-646.493) -- 0:02:48 257000 -- [-650.546] (-646.489) (-650.061) (-655.566) * (-657.839) (-655.493) (-653.932) [-652.814] -- 0:02:47 258000 -- (-662.183) (-657.086) [-661.097] (-655.619) * (-658.059) (-651.452) (-654.563) [-659.495] -- 0:02:46 259000 -- (-653.985) (-663.499) (-653.651) [-662.783] * [-658.924] (-654.899) (-656.931) (-659.094) -- 0:02:45 260000 -- (-647.263) [-650.621] (-653.922) (-652.534) * (-652.304) (-660.933) (-650.000) [-653.091] -- 0:02:47 Average standard deviation of split frequencies: 0.018860 261000 -- (-648.382) (-653.667) [-651.803] (-652.350) * (-654.572) [-652.798] (-656.409) (-652.633) -- 0:02:47 262000 -- (-650.774) [-650.375] (-658.249) (-664.850) * (-654.608) (-652.000) [-649.790] (-657.124) -- 0:02:46 263000 -- [-644.692] (-653.338) (-656.958) (-658.873) * [-647.245] (-652.845) (-654.744) (-656.851) -- 0:02:45 264000 -- [-649.803] (-650.024) (-658.843) (-651.842) * [-653.603] (-652.052) (-650.848) (-659.042) -- 0:02:47 265000 -- (-663.719) [-654.939] (-654.168) (-659.352) * [-654.980] (-658.082) (-649.540) (-653.036) -- 0:02:46 Average standard deviation of split frequencies: 0.019494 266000 -- [-658.204] (-654.734) (-660.756) (-664.224) * (-651.808) [-653.387] (-653.906) (-651.726) -- 0:02:45 267000 -- (-659.494) [-652.725] (-649.422) (-656.090) * [-654.978] (-651.901) (-651.403) (-659.069) -- 0:02:44 268000 -- [-656.387] (-650.722) (-649.701) (-661.014) * (-647.284) [-648.783] (-649.925) (-653.127) -- 0:02:43 269000 -- (-660.104) [-646.166] (-650.680) (-661.924) * (-655.596) (-651.036) [-653.194] (-649.922) -- 0:02:45 270000 -- (-655.121) [-652.840] (-647.933) (-656.356) * [-648.451] (-653.654) (-655.828) (-652.988) -- 0:02:44 Average standard deviation of split frequencies: 0.018163 271000 -- (-669.467) (-659.991) [-650.457] (-658.718) * (-655.432) [-649.703] (-659.128) (-655.822) -- 0:02:44 272000 -- (-658.408) [-653.210] (-660.442) (-656.931) * (-654.627) (-658.963) [-655.020] (-658.407) -- 0:02:43 273000 -- (-657.340) [-650.197] (-658.955) (-650.980) * (-657.483) [-652.026] (-652.471) (-650.945) -- 0:02:45 274000 -- (-663.870) (-646.587) [-650.956] (-651.087) * (-657.560) [-648.449] (-658.264) (-657.638) -- 0:02:44 275000 -- (-651.218) [-649.846] (-662.381) (-657.225) * [-651.608] (-662.683) (-651.262) (-650.560) -- 0:02:43 Average standard deviation of split frequencies: 0.019357 276000 -- (-654.774) [-655.900] (-656.652) (-657.712) * (-660.270) (-656.284) [-650.113] (-657.944) -- 0:02:42 277000 -- (-649.617) (-649.033) (-661.785) [-651.611] * (-653.330) (-660.974) (-656.129) [-652.106] -- 0:02:41 278000 -- (-648.726) (-652.848) (-652.976) [-658.877] * [-652.664] (-655.919) (-660.037) (-655.747) -- 0:02:43 279000 -- (-651.673) (-652.929) (-653.111) [-650.374] * (-661.508) (-654.315) (-656.542) [-658.703] -- 0:02:42 280000 -- (-656.177) [-653.033] (-659.583) (-651.259) * [-653.023] (-654.260) (-666.867) (-649.960) -- 0:02:42 Average standard deviation of split frequencies: 0.017156 281000 -- (-646.361) (-653.339) (-654.670) [-651.798] * (-653.256) [-655.182] (-657.766) (-650.149) -- 0:02:41 282000 -- (-654.762) (-658.176) [-649.338] (-653.061) * (-649.242) (-659.000) (-649.561) [-656.790] -- 0:02:42 283000 -- [-649.084] (-654.676) (-657.316) (-649.708) * [-647.362] (-658.602) (-653.155) (-660.426) -- 0:02:42 284000 -- [-655.527] (-659.978) (-648.110) (-656.186) * (-659.808) (-653.493) [-648.930] (-662.943) -- 0:02:41 285000 -- (-653.668) (-656.534) (-653.431) [-651.244] * [-646.728] (-656.323) (-661.895) (-650.685) -- 0:02:40 Average standard deviation of split frequencies: 0.017911 286000 -- [-647.835] (-661.822) (-658.618) (-652.666) * (-651.858) (-647.431) (-654.221) [-655.343] -- 0:02:42 287000 -- (-655.570) [-650.691] (-663.734) (-657.718) * (-669.289) [-654.810] (-650.990) (-654.788) -- 0:02:41 288000 -- (-657.567) [-654.103] (-661.761) (-654.049) * [-654.832] (-653.685) (-649.741) (-653.600) -- 0:02:40 289000 -- (-650.689) [-653.007] (-666.192) (-654.579) * [-651.484] (-653.450) (-652.611) (-657.374) -- 0:02:39 290000 -- (-662.370) [-648.238] (-655.256) (-651.208) * [-646.993] (-662.218) (-651.787) (-650.047) -- 0:02:39 Average standard deviation of split frequencies: 0.015986 291000 -- (-662.679) [-649.680] (-655.489) (-658.257) * [-650.871] (-656.362) (-662.585) (-649.445) -- 0:02:40 292000 -- (-654.698) (-647.628) (-654.000) [-651.935] * (-651.007) [-647.417] (-653.235) (-648.342) -- 0:02:40 293000 -- (-664.435) (-651.453) (-661.202) [-649.364] * (-650.333) [-648.392] (-658.357) (-649.228) -- 0:02:39 294000 -- (-666.804) (-662.385) (-651.802) [-661.303] * [-657.217] (-659.432) (-652.231) (-663.137) -- 0:02:38 295000 -- (-652.790) (-665.804) (-652.860) [-658.282] * (-651.012) [-653.296] (-659.530) (-653.765) -- 0:02:40 Average standard deviation of split frequencies: 0.014447 296000 -- [-648.720] (-652.119) (-661.232) (-656.819) * [-655.745] (-652.580) (-655.120) (-652.528) -- 0:02:39 297000 -- (-646.785) (-656.702) [-647.052] (-658.351) * (-651.499) [-650.418] (-653.610) (-649.146) -- 0:02:38 298000 -- (-654.134) (-652.812) [-647.654] (-664.932) * (-652.195) (-657.317) [-647.980] (-647.625) -- 0:02:37 299000 -- (-658.786) (-658.314) (-653.368) [-658.554] * (-652.116) (-660.858) (-657.442) [-652.018] -- 0:02:37 300000 -- (-661.904) (-663.789) [-652.759] (-659.410) * (-650.361) [-650.664] (-651.830) (-647.972) -- 0:02:38 Average standard deviation of split frequencies: 0.013775 301000 -- (-656.001) (-651.358) (-663.890) [-658.230] * (-652.485) (-654.362) [-651.897] (-660.295) -- 0:02:37 302000 -- [-648.290] (-652.250) (-658.451) (-666.050) * (-666.095) (-648.911) (-654.119) [-650.271] -- 0:02:37 303000 -- (-652.479) (-656.891) (-660.098) [-651.563] * (-655.991) (-656.408) (-659.731) [-650.761] -- 0:02:36 304000 -- [-649.140] (-652.931) (-650.113) (-652.157) * (-652.851) [-656.636] (-657.026) (-658.066) -- 0:02:37 305000 -- (-651.152) (-650.347) (-657.059) [-649.298] * (-655.819) (-651.991) (-651.384) [-649.650] -- 0:02:37 Average standard deviation of split frequencies: 0.013645 306000 -- (-659.087) [-650.601] (-655.645) (-656.819) * [-653.536] (-654.943) (-662.050) (-653.026) -- 0:02:36 307000 -- [-652.880] (-651.777) (-661.952) (-654.832) * (-660.539) (-658.206) (-655.215) [-650.935] -- 0:02:35 308000 -- (-656.714) [-648.867] (-660.673) (-657.217) * (-651.781) [-651.179] (-657.926) (-656.543) -- 0:02:35 309000 -- [-652.551] (-657.633) (-652.987) (-657.621) * [-656.346] (-654.387) (-664.323) (-650.036) -- 0:02:36 310000 -- (-656.087) (-656.371) (-663.521) [-648.011] * (-651.389) (-651.130) [-657.206] (-652.241) -- 0:02:35 Average standard deviation of split frequencies: 0.013151 311000 -- (-646.629) (-646.484) [-655.959] (-649.706) * (-660.829) (-651.246) (-664.243) [-658.624] -- 0:02:35 312000 -- (-654.385) (-650.718) (-657.513) [-646.668] * (-662.123) (-649.636) (-661.188) [-657.217] -- 0:02:34 313000 -- (-653.940) (-649.005) (-666.169) [-649.905] * (-653.620) (-653.757) [-652.394] (-649.741) -- 0:02:35 314000 -- [-645.544] (-652.041) (-656.534) (-655.816) * (-664.347) [-664.776] (-661.002) (-659.274) -- 0:02:35 315000 -- (-656.688) (-671.276) (-653.324) [-651.099] * (-657.839) (-649.739) [-649.019] (-657.757) -- 0:02:34 Average standard deviation of split frequencies: 0.012929 316000 -- [-658.646] (-657.058) (-660.063) (-651.209) * [-655.352] (-654.223) (-652.104) (-654.741) -- 0:02:33 317000 -- (-655.594) (-658.234) [-650.591] (-654.433) * (-662.421) [-651.793] (-667.425) (-652.987) -- 0:02:32 318000 -- (-666.719) (-654.043) [-654.938] (-651.020) * (-661.290) (-651.768) [-646.176] (-656.943) -- 0:02:34 319000 -- (-657.767) (-650.225) (-647.403) [-651.488] * (-660.245) (-661.109) (-652.756) [-652.442] -- 0:02:33 320000 -- (-661.843) (-655.074) (-655.007) [-650.371] * (-650.470) (-660.381) [-654.801] (-650.786) -- 0:02:33 Average standard deviation of split frequencies: 0.012839 321000 -- (-660.709) (-653.274) (-648.897) [-649.566] * (-650.570) (-655.538) [-655.477] (-663.622) -- 0:02:32 322000 -- (-666.108) [-655.893] (-650.092) (-654.040) * (-664.876) [-647.472] (-652.096) (-653.990) -- 0:02:33 323000 -- (-662.108) (-647.905) (-651.581) [-649.487] * (-664.238) (-649.513) [-649.429] (-649.401) -- 0:02:33 324000 -- (-661.864) (-650.410) [-650.018] (-654.258) * (-657.419) (-650.580) [-647.767] (-654.858) -- 0:02:32 325000 -- (-663.429) [-649.740] (-650.213) (-645.860) * (-652.463) (-654.908) (-666.764) [-655.955] -- 0:02:31 Average standard deviation of split frequencies: 0.012725 326000 -- (-665.195) (-653.524) [-644.758] (-654.627) * (-651.905) (-649.039) [-656.170] (-650.795) -- 0:02:32 327000 -- (-659.103) [-653.239] (-655.697) (-651.598) * [-653.016] (-651.592) (-652.064) (-650.608) -- 0:02:32 328000 -- (-666.085) (-650.444) [-649.930] (-661.162) * (-652.860) [-660.379] (-658.407) (-658.208) -- 0:02:31 329000 -- (-653.373) (-651.985) [-650.465] (-653.681) * (-649.675) [-649.954] (-657.607) (-662.021) -- 0:02:30 330000 -- (-658.511) (-652.243) (-649.881) [-648.446] * (-665.484) [-654.777] (-657.079) (-654.547) -- 0:02:30 Average standard deviation of split frequencies: 0.011310 331000 -- (-658.758) (-656.918) [-659.535] (-651.324) * (-655.243) (-648.348) [-650.160] (-653.875) -- 0:02:31 332000 -- (-651.797) (-651.567) [-652.554] (-660.298) * (-649.843) [-653.968] (-648.358) (-667.922) -- 0:02:30 333000 -- (-659.644) (-656.946) [-653.148] (-660.001) * [-654.724] (-651.179) (-661.631) (-653.783) -- 0:02:30 334000 -- (-661.835) (-651.634) (-653.054) [-656.745] * (-652.489) [-648.295] (-659.779) (-666.736) -- 0:02:29 335000 -- (-654.947) (-649.063) [-653.104] (-654.340) * [-651.246] (-655.111) (-652.706) (-659.860) -- 0:02:30 Average standard deviation of split frequencies: 0.011598 336000 -- (-649.366) [-647.516] (-660.502) (-668.726) * (-654.589) (-664.543) (-648.285) [-660.832] -- 0:02:30 337000 -- [-646.775] (-655.171) (-652.260) (-657.405) * [-652.557] (-652.761) (-656.867) (-660.897) -- 0:02:29 338000 -- [-652.178] (-655.993) (-659.048) (-654.090) * [-647.201] (-654.592) (-655.989) (-657.589) -- 0:02:28 339000 -- (-649.442) (-658.026) [-653.137] (-655.401) * [-655.472] (-653.451) (-660.296) (-651.580) -- 0:02:30 340000 -- (-652.017) [-653.905] (-653.903) (-648.726) * [-650.754] (-653.520) (-656.344) (-650.746) -- 0:02:29 Average standard deviation of split frequencies: 0.012362 341000 -- (-662.424) (-652.499) (-653.180) [-655.497] * (-652.671) (-656.264) [-653.461] (-660.881) -- 0:02:28 342000 -- (-658.831) [-652.346] (-655.927) (-649.699) * (-650.996) (-665.203) [-643.398] (-648.826) -- 0:02:28 343000 -- [-649.845] (-669.007) (-656.798) (-650.153) * (-655.133) (-655.336) [-650.996] (-653.070) -- 0:02:29 344000 -- (-648.201) (-657.433) (-651.743) [-654.816] * [-651.984] (-655.103) (-649.252) (-650.071) -- 0:02:28 345000 -- [-648.929] (-655.323) (-659.712) (-649.097) * [-649.844] (-647.044) (-656.986) (-654.556) -- 0:02:28 Average standard deviation of split frequencies: 0.012716 346000 -- (-649.368) [-649.159] (-659.021) (-653.284) * (-655.570) (-653.589) (-651.138) [-651.939] -- 0:02:27 347000 -- (-651.954) (-651.790) [-652.460] (-652.440) * [-655.930] (-652.382) (-655.838) (-654.243) -- 0:02:26 348000 -- [-658.453] (-650.127) (-649.900) (-656.888) * (-661.006) (-649.588) [-652.885] (-667.661) -- 0:02:28 349000 -- (-650.544) (-651.982) (-653.794) [-646.919] * (-650.675) (-656.055) (-653.163) [-658.605] -- 0:02:27 350000 -- (-652.681) (-652.147) [-653.177] (-662.044) * (-660.316) (-656.664) [-648.748] (-655.993) -- 0:02:26 Average standard deviation of split frequencies: 0.013155 351000 -- [-649.452] (-651.894) (-659.568) (-661.945) * (-655.780) (-653.120) (-658.965) [-657.131] -- 0:02:26 352000 -- (-658.618) [-651.454] (-653.901) (-651.417) * (-654.175) (-654.110) (-653.707) [-655.747] -- 0:02:27 353000 -- (-651.770) [-655.486] (-656.492) (-651.051) * (-655.256) (-652.596) [-647.694] (-658.659) -- 0:02:26 354000 -- (-664.255) (-654.717) (-646.645) [-655.118] * [-655.043] (-658.178) (-654.077) (-656.653) -- 0:02:25 355000 -- (-662.098) (-650.982) [-648.521] (-649.679) * (-658.393) [-650.433] (-644.843) (-656.206) -- 0:02:25 Average standard deviation of split frequencies: 0.013053 356000 -- (-666.793) [-653.238] (-660.221) (-659.944) * [-652.827] (-654.277) (-652.924) (-652.507) -- 0:02:26 357000 -- [-655.210] (-648.469) (-658.065) (-660.150) * (-655.807) (-657.126) [-653.145] (-655.726) -- 0:02:25 358000 -- (-658.610) (-655.794) [-645.636] (-663.544) * (-647.749) (-649.678) [-652.371] (-657.607) -- 0:02:25 359000 -- (-652.358) (-649.892) (-654.209) [-650.833] * [-656.854] (-655.252) (-649.395) (-653.782) -- 0:02:24 360000 -- (-650.952) (-653.464) (-651.705) [-653.292] * (-652.930) (-651.731) (-650.146) [-654.166] -- 0:02:24 Average standard deviation of split frequencies: 0.013164 361000 -- (-661.199) [-651.563] (-656.100) (-654.965) * (-655.654) (-656.902) [-650.881] (-666.710) -- 0:02:25 362000 -- (-655.499) (-650.153) (-671.797) [-653.531] * [-647.666] (-652.789) (-650.110) (-659.369) -- 0:02:24 363000 -- (-661.944) [-656.912] (-653.229) (-657.230) * (-658.601) (-650.967) [-651.011] (-653.940) -- 0:02:23 364000 -- (-659.848) [-654.317] (-661.815) (-663.718) * (-651.887) (-658.627) [-648.556] (-651.984) -- 0:02:23 365000 -- (-661.842) (-654.951) (-651.821) [-649.448] * (-655.183) (-655.695) (-659.524) [-653.522] -- 0:02:24 Average standard deviation of split frequencies: 0.012972 366000 -- (-665.966) (-649.331) (-651.154) [-653.329] * (-648.068) (-664.212) (-651.006) [-648.490] -- 0:02:23 367000 -- (-655.353) (-653.362) [-649.750] (-662.112) * [-653.979] (-654.569) (-655.087) (-647.991) -- 0:02:23 368000 -- [-651.718] (-661.662) (-657.075) (-650.754) * [-652.053] (-655.823) (-659.995) (-650.017) -- 0:02:22 369000 -- (-650.578) [-660.418] (-661.292) (-662.558) * (-656.979) (-657.164) (-653.758) [-654.556] -- 0:02:23 370000 -- (-659.006) [-654.206] (-654.041) (-648.879) * [-649.457] (-647.710) (-656.523) (-651.787) -- 0:02:23 Average standard deviation of split frequencies: 0.012972 371000 -- (-648.734) (-661.061) (-648.253) [-649.395] * (-649.449) (-653.314) [-644.897] (-662.610) -- 0:02:22 372000 -- (-661.945) (-659.333) [-651.566] (-661.896) * (-653.744) [-648.382] (-654.641) (-660.859) -- 0:02:21 373000 -- (-656.303) [-657.614] (-655.337) (-657.271) * (-657.412) [-652.210] (-659.089) (-650.000) -- 0:02:21 374000 -- (-652.642) (-662.034) [-660.411] (-651.299) * (-658.945) (-644.174) (-651.343) [-651.932] -- 0:02:22 375000 -- (-650.392) (-657.998) [-652.133] (-653.320) * (-654.638) [-650.270] (-659.048) (-657.355) -- 0:02:21 Average standard deviation of split frequencies: 0.014659 376000 -- [-653.994] (-665.177) (-657.382) (-659.168) * [-652.794] (-652.020) (-660.886) (-666.438) -- 0:02:21 377000 -- (-652.379) (-656.524) (-652.782) [-651.929] * (-657.945) [-652.675] (-654.429) (-662.059) -- 0:02:20 378000 -- (-649.228) [-656.772] (-650.935) (-651.270) * (-647.375) (-655.091) [-655.530] (-654.629) -- 0:02:21 379000 -- [-649.817] (-652.731) (-652.822) (-652.898) * (-648.904) (-647.345) [-650.514] (-668.457) -- 0:02:20 380000 -- [-649.942] (-658.090) (-655.631) (-652.218) * (-652.304) [-653.640] (-660.037) (-651.419) -- 0:02:20 Average standard deviation of split frequencies: 0.014384 381000 -- [-646.948] (-652.256) (-651.153) (-657.228) * (-654.749) [-651.526] (-653.108) (-650.226) -- 0:02:19 382000 -- (-653.511) (-651.165) (-656.721) [-655.536] * [-648.975] (-654.434) (-658.158) (-653.641) -- 0:02:19 383000 -- (-649.872) (-652.903) (-658.491) [-651.540] * (-651.405) (-649.582) (-652.423) [-651.778] -- 0:02:20 384000 -- (-652.152) (-659.439) (-663.270) [-653.445] * [-646.063] (-657.051) (-653.702) (-668.125) -- 0:02:19 385000 -- (-652.710) (-650.799) [-655.291] (-646.250) * [-645.039] (-653.039) (-656.148) (-658.376) -- 0:02:18 Average standard deviation of split frequencies: 0.014248 386000 -- (-660.733) [-656.929] (-656.663) (-653.306) * (-658.167) (-655.335) [-654.721] (-659.384) -- 0:02:18 387000 -- [-653.653] (-656.871) (-653.456) (-656.253) * (-650.690) [-655.727] (-673.688) (-661.561) -- 0:02:19 388000 -- [-651.283] (-655.392) (-651.773) (-650.855) * (-655.932) (-658.798) [-655.046] (-655.276) -- 0:02:18 389000 -- [-649.924] (-659.472) (-650.431) (-653.712) * (-658.610) (-649.693) [-645.588] (-656.541) -- 0:02:18 390000 -- (-651.244) (-660.773) [-649.201] (-654.740) * (-660.739) (-659.958) [-653.343] (-655.166) -- 0:02:17 Average standard deviation of split frequencies: 0.013704 391000 -- (-656.372) (-646.519) (-653.053) [-656.903] * [-658.351] (-663.285) (-648.166) (-655.153) -- 0:02:17 392000 -- (-646.501) (-653.309) [-650.705] (-659.344) * [-644.024] (-657.626) (-658.855) (-653.154) -- 0:02:18 393000 -- (-654.194) (-659.554) [-654.292] (-653.825) * (-650.379) (-654.156) (-649.798) [-658.518] -- 0:02:17 394000 -- [-651.517] (-649.600) (-652.700) (-656.688) * (-653.064) (-660.565) [-645.599] (-662.129) -- 0:02:16 395000 -- (-651.274) [-651.764] (-651.609) (-652.993) * (-657.224) (-667.939) (-660.655) [-651.907] -- 0:02:16 Average standard deviation of split frequencies: 0.014926 396000 -- (-648.468) (-649.503) (-657.488) [-649.628] * (-661.305) (-663.599) [-649.745] (-649.525) -- 0:02:17 397000 -- [-652.089] (-649.648) (-654.848) (-654.124) * (-659.203) (-658.868) (-658.360) [-649.298] -- 0:02:16 398000 -- (-657.085) (-658.086) (-653.254) [-647.910] * (-653.214) (-654.959) (-649.407) [-646.786] -- 0:02:16 399000 -- (-657.311) (-654.135) [-653.594] (-663.020) * (-653.837) (-657.599) (-653.521) [-649.260] -- 0:02:15 400000 -- (-656.095) (-651.983) (-667.660) [-650.826] * (-652.653) (-659.363) [-646.980] (-650.477) -- 0:02:15 Average standard deviation of split frequencies: 0.015205 401000 -- [-647.966] (-650.427) (-652.068) (-656.755) * (-668.404) [-657.486] (-650.465) (-651.329) -- 0:02:15 402000 -- (-653.139) [-647.484] (-650.047) (-653.582) * (-663.087) [-656.126] (-647.945) (-657.656) -- 0:02:15 403000 -- (-660.323) (-655.176) [-649.346] (-650.646) * (-651.276) (-660.976) (-650.063) [-652.128] -- 0:02:14 404000 -- [-655.516] (-651.934) (-651.564) (-651.613) * (-654.306) (-652.381) [-648.990] (-661.486) -- 0:02:14 405000 -- (-659.492) [-658.418] (-650.315) (-660.985) * [-649.081] (-653.222) (-656.980) (-655.274) -- 0:02:15 Average standard deviation of split frequencies: 0.014265 406000 -- [-653.986] (-651.147) (-654.942) (-650.606) * (-658.060) (-656.186) [-648.845] (-661.539) -- 0:02:14 407000 -- (-657.679) (-656.925) [-657.448] (-650.832) * (-656.017) (-650.169) [-645.877] (-659.395) -- 0:02:14 408000 -- (-654.576) (-658.355) (-655.486) [-658.365] * (-659.470) [-651.405] (-647.517) (-656.899) -- 0:02:13 409000 -- (-662.530) (-655.220) (-649.686) [-651.486] * (-663.113) (-653.196) (-648.247) [-649.555] -- 0:02:14 410000 -- [-652.645] (-658.565) (-650.349) (-653.517) * (-660.310) (-645.325) (-650.312) [-655.725] -- 0:02:13 Average standard deviation of split frequencies: 0.014759 411000 -- (-663.601) (-654.681) [-649.356] (-651.887) * (-652.297) (-659.280) [-651.271] (-654.422) -- 0:02:13 412000 -- (-660.387) (-651.400) (-652.988) [-650.980] * (-657.897) (-662.782) [-650.266] (-657.716) -- 0:02:12 413000 -- (-660.534) [-652.144] (-659.793) (-649.131) * [-653.240] (-652.097) (-653.466) (-654.781) -- 0:02:12 414000 -- (-669.354) (-658.592) [-647.949] (-659.166) * (-655.229) (-658.423) [-652.917] (-654.080) -- 0:02:13 415000 -- (-657.198) (-661.783) (-652.114) [-652.597] * (-661.178) (-649.931) [-651.190] (-661.049) -- 0:02:12 Average standard deviation of split frequencies: 0.014812 416000 -- (-659.503) (-653.669) [-649.060] (-652.522) * (-664.340) (-652.302) [-650.998] (-647.045) -- 0:02:11 417000 -- (-652.603) (-661.281) (-650.114) [-652.190] * (-650.271) [-654.191] (-650.578) (-649.687) -- 0:02:11 418000 -- (-673.392) (-651.001) (-648.703) [-652.446] * (-650.994) (-653.931) (-652.407) [-649.571] -- 0:02:12 419000 -- (-661.337) (-655.266) (-653.311) [-652.021] * (-657.266) (-653.863) [-653.617] (-660.393) -- 0:02:11 420000 -- [-651.409] (-668.381) (-649.239) (-655.382) * [-656.102] (-654.101) (-667.186) (-651.686) -- 0:02:11 Average standard deviation of split frequencies: 0.014888 421000 -- (-654.634) (-674.802) (-654.409) [-652.023] * (-653.972) (-648.165) [-648.307] (-652.805) -- 0:02:10 422000 -- (-647.525) (-652.104) [-650.787] (-650.026) * (-652.151) (-652.748) [-649.495] (-650.480) -- 0:02:10 423000 -- (-645.557) (-658.025) (-653.320) [-650.528] * (-654.157) (-652.426) [-656.452] (-650.074) -- 0:02:10 424000 -- (-659.083) (-655.538) [-645.054] (-659.030) * (-654.136) (-658.218) [-647.730] (-653.769) -- 0:02:10 425000 -- (-661.081) (-657.416) (-655.197) [-654.348] * (-653.644) [-650.852] (-655.236) (-653.902) -- 0:02:09 Average standard deviation of split frequencies: 0.014465 426000 -- [-653.588] (-649.776) (-651.956) (-660.139) * (-653.229) (-650.296) (-652.405) [-646.318] -- 0:02:09 427000 -- (-663.307) (-657.076) [-652.681] (-651.861) * (-658.684) (-660.183) (-654.789) [-651.747] -- 0:02:10 428000 -- (-660.295) (-650.337) [-650.094] (-652.345) * [-660.122] (-650.983) (-665.270) (-656.975) -- 0:02:09 429000 -- (-654.939) [-654.954] (-650.253) (-653.728) * (-650.494) (-661.298) [-650.654] (-650.032) -- 0:02:09 430000 -- (-657.908) (-655.223) [-652.056] (-645.731) * (-661.461) (-655.310) [-649.901] (-650.746) -- 0:02:08 Average standard deviation of split frequencies: 0.014152 431000 -- (-653.156) (-654.454) (-665.326) [-650.785] * (-655.285) (-655.018) [-653.095] (-651.824) -- 0:02:08 432000 -- (-651.612) [-654.708] (-654.847) (-654.980) * [-652.667] (-646.472) (-657.043) (-650.367) -- 0:02:08 433000 -- (-649.357) [-651.267] (-655.185) (-654.637) * (-665.107) (-651.879) [-653.469] (-647.421) -- 0:02:08 434000 -- [-649.481] (-651.187) (-662.610) (-654.205) * (-664.971) (-655.471) (-659.185) [-648.784] -- 0:02:07 435000 -- (-659.617) (-653.712) (-649.020) [-651.775] * (-661.612) (-651.741) (-652.983) [-648.263] -- 0:02:07 Average standard deviation of split frequencies: 0.013747 436000 -- (-651.868) (-654.713) [-650.439] (-661.757) * (-655.336) [-651.556] (-661.923) (-652.806) -- 0:02:08 437000 -- (-664.838) [-651.975] (-646.427) (-653.380) * (-662.770) [-648.479] (-656.916) (-659.655) -- 0:02:07 438000 -- (-650.939) (-653.644) (-647.025) [-652.386] * (-668.036) [-651.365] (-656.874) (-658.869) -- 0:02:07 439000 -- (-652.768) (-649.341) (-654.222) [-653.098] * (-663.626) (-647.914) [-651.907] (-650.822) -- 0:02:06 440000 -- (-646.458) [-651.447] (-654.772) (-656.939) * (-662.039) [-650.291] (-650.153) (-654.096) -- 0:02:07 Average standard deviation of split frequencies: 0.013372 441000 -- (-657.872) (-657.798) [-658.989] (-651.044) * (-660.940) (-658.720) (-655.900) [-651.736] -- 0:02:06 442000 -- (-651.609) (-656.724) [-658.825] (-649.138) * (-650.011) (-651.352) (-661.082) [-650.182] -- 0:02:06 443000 -- [-651.118] (-650.890) (-658.193) (-653.392) * [-651.633] (-661.529) (-658.760) (-652.120) -- 0:02:05 444000 -- (-656.954) (-653.707) [-655.035] (-663.285) * (-661.237) (-651.263) (-659.601) [-656.489] -- 0:02:05 445000 -- (-656.781) (-660.332) [-649.786] (-650.544) * (-659.007) (-650.502) [-655.534] (-663.005) -- 0:02:05 Average standard deviation of split frequencies: 0.013212 446000 -- (-654.352) (-662.411) [-650.770] (-652.086) * (-659.699) [-656.702] (-656.829) (-653.215) -- 0:02:05 447000 -- (-652.803) (-655.425) (-653.933) [-651.438] * [-652.864] (-652.614) (-655.069) (-646.794) -- 0:02:04 448000 -- [-652.291] (-660.557) (-655.620) (-658.760) * [-651.238] (-653.191) (-655.572) (-648.415) -- 0:02:04 449000 -- (-656.371) (-662.993) (-662.439) [-647.997] * (-660.419) (-651.372) (-658.374) [-649.953] -- 0:02:05 450000 -- (-649.618) (-653.854) [-650.346] (-650.810) * (-659.836) (-655.362) (-665.584) [-648.126] -- 0:02:04 Average standard deviation of split frequencies: 0.013225 451000 -- (-649.975) [-653.687] (-648.065) (-653.668) * (-657.602) (-653.415) [-656.309] (-653.505) -- 0:02:04 452000 -- (-651.071) (-658.616) (-650.697) [-650.692] * (-659.947) (-656.368) [-656.870] (-652.059) -- 0:02:03 453000 -- (-655.250) (-658.184) (-653.646) [-657.679] * [-646.752] (-654.267) (-652.111) (-660.080) -- 0:02:03 454000 -- [-647.802] (-659.713) (-649.674) (-651.741) * (-657.585) (-649.892) (-657.527) [-653.753] -- 0:02:03 455000 -- [-652.387] (-648.880) (-651.542) (-653.715) * [-652.917] (-670.588) (-647.925) (-651.085) -- 0:02:03 Average standard deviation of split frequencies: 0.013218 456000 -- [-651.664] (-653.085) (-666.683) (-650.834) * (-652.840) [-650.115] (-674.785) (-661.064) -- 0:02:02 457000 -- [-657.430] (-654.609) (-653.532) (-650.805) * [-651.660] (-650.967) (-666.918) (-655.471) -- 0:02:02 458000 -- [-655.949] (-656.435) (-655.878) (-656.747) * (-655.402) (-650.958) (-654.905) [-647.314] -- 0:02:03 459000 -- (-652.057) [-652.791] (-654.231) (-652.323) * (-655.405) (-652.926) (-658.821) [-657.308] -- 0:02:02 460000 -- (-657.453) (-650.174) [-651.126] (-673.224) * (-653.380) [-650.416] (-654.278) (-652.995) -- 0:02:02 Average standard deviation of split frequencies: 0.013157 461000 -- (-652.996) (-655.105) (-650.599) [-652.366] * (-660.515) (-654.597) [-648.642] (-652.413) -- 0:02:01 462000 -- (-664.825) [-648.485] (-654.283) (-659.914) * (-653.659) [-646.493] (-669.115) (-653.996) -- 0:02:02 463000 -- (-648.684) (-646.955) (-651.914) [-648.741] * (-656.638) (-655.664) [-654.286] (-648.434) -- 0:02:01 464000 -- (-654.663) [-650.062] (-649.629) (-655.218) * (-658.483) (-648.396) [-659.310] (-651.189) -- 0:02:01 465000 -- (-656.560) (-653.618) (-651.031) [-647.103] * (-653.460) (-661.965) [-650.028] (-649.907) -- 0:02:00 Average standard deviation of split frequencies: 0.013440 466000 -- (-652.872) [-651.907] (-660.692) (-659.964) * (-660.405) (-656.207) [-658.820] (-655.167) -- 0:02:00 467000 -- (-661.389) [-647.858] (-650.435) (-659.522) * (-652.884) (-664.600) [-650.114] (-655.206) -- 0:02:00 468000 -- (-656.576) (-653.335) [-657.060] (-654.239) * (-650.465) (-651.845) [-651.173] (-650.401) -- 0:02:00 469000 -- (-658.343) (-657.074) (-651.258) [-647.965] * (-650.631) (-654.148) [-653.097] (-648.262) -- 0:02:00 470000 -- (-657.343) (-657.134) [-652.059] (-659.753) * (-648.166) (-655.294) [-653.638] (-653.112) -- 0:01:59 Average standard deviation of split frequencies: 0.013163 471000 -- (-667.357) (-657.188) [-649.957] (-654.058) * (-647.729) [-653.692] (-656.360) (-645.034) -- 0:02:00 472000 -- (-661.892) (-654.429) (-653.758) [-651.028] * (-651.999) (-653.938) (-657.083) [-650.654] -- 0:01:59 473000 -- (-662.119) (-659.481) (-659.701) [-647.602] * (-661.270) (-658.249) (-653.201) [-648.481] -- 0:01:59 474000 -- [-650.169] (-656.655) (-654.112) (-655.502) * [-656.117] (-665.728) (-657.769) (-656.124) -- 0:01:58 475000 -- [-652.281] (-655.396) (-650.959) (-659.880) * [-648.148] (-668.708) (-659.553) (-650.893) -- 0:01:59 Average standard deviation of split frequencies: 0.011884 476000 -- [-655.949] (-656.117) (-651.626) (-660.099) * [-647.074] (-671.568) (-657.185) (-649.753) -- 0:01:58 477000 -- (-660.325) (-661.071) (-652.449) [-650.654] * (-655.265) (-665.736) (-651.856) [-648.190] -- 0:01:58 478000 -- (-647.174) (-663.735) [-653.256] (-657.544) * (-660.647) (-659.485) [-655.137] (-646.778) -- 0:01:57 479000 -- (-655.436) (-650.506) [-656.029] (-658.731) * (-661.859) (-661.841) (-654.977) [-653.174] -- 0:01:57 480000 -- (-657.031) (-655.298) [-653.412] (-659.428) * (-648.048) (-659.257) [-654.201] (-659.289) -- 0:01:58 Average standard deviation of split frequencies: 0.011769 481000 -- (-668.449) [-648.834] (-664.628) (-651.180) * (-648.796) (-660.435) (-657.853) [-648.994] -- 0:01:57 482000 -- (-655.538) (-650.334) (-652.777) [-648.128] * (-652.188) (-655.994) (-657.208) [-653.323] -- 0:01:57 483000 -- (-650.016) (-661.365) (-655.720) [-651.798] * (-653.392) (-656.705) (-650.451) [-651.425] -- 0:01:56 484000 -- (-648.890) [-649.245] (-659.513) (-653.287) * (-654.675) (-656.214) [-647.748] (-651.411) -- 0:01:57 485000 -- [-649.984] (-654.317) (-660.321) (-657.800) * (-651.055) [-653.520] (-658.135) (-657.556) -- 0:01:56 Average standard deviation of split frequencies: 0.011917 486000 -- (-653.208) [-650.800] (-651.132) (-657.238) * (-648.192) (-652.329) (-649.607) [-650.585] -- 0:01:56 487000 -- (-650.532) [-653.628] (-646.985) (-652.477) * (-655.461) [-652.109] (-651.242) (-665.514) -- 0:01:55 488000 -- [-644.353] (-650.522) (-664.455) (-649.520) * (-660.082) (-659.554) [-649.653] (-659.178) -- 0:01:55 489000 -- (-654.456) (-664.902) (-655.019) [-655.641] * [-646.853] (-656.751) (-653.900) (-659.749) -- 0:01:55 490000 -- (-651.392) (-659.647) [-651.007] (-650.845) * [-646.560] (-653.382) (-652.411) (-656.197) -- 0:01:55 Average standard deviation of split frequencies: 0.011529 491000 -- (-659.618) (-654.801) (-650.037) [-653.454] * (-646.908) (-650.899) (-651.858) [-650.375] -- 0:01:55 492000 -- [-651.105] (-652.084) (-655.963) (-654.144) * [-656.742] (-645.279) (-656.351) (-653.238) -- 0:01:54 493000 -- (-652.474) (-659.421) [-651.775] (-669.915) * (-650.662) [-651.377] (-657.662) (-651.204) -- 0:01:55 494000 -- (-653.704) (-652.336) [-646.970] (-662.403) * (-655.704) (-656.308) [-648.268] (-652.196) -- 0:01:54 495000 -- (-646.811) [-653.747] (-654.851) (-658.158) * (-668.367) [-648.546] (-655.284) (-657.776) -- 0:01:54 Average standard deviation of split frequencies: 0.012152 496000 -- (-661.466) (-658.090) (-645.635) [-651.657] * (-652.741) (-652.356) [-658.583] (-656.722) -- 0:01:53 497000 -- (-673.493) (-653.366) [-651.449] (-656.279) * (-658.067) [-652.658] (-650.909) (-655.809) -- 0:01:53 498000 -- [-651.491] (-661.536) (-647.909) (-652.805) * (-654.282) [-648.622] (-653.634) (-654.122) -- 0:01:53 499000 -- (-656.130) (-654.991) [-660.142] (-653.693) * [-648.262] (-647.983) (-649.037) (-648.998) -- 0:01:53 500000 -- (-654.928) (-652.290) (-660.419) [-651.952] * (-648.483) (-652.458) (-652.021) [-651.912] -- 0:01:53 Average standard deviation of split frequencies: 0.011904 501000 -- [-653.330] (-657.524) (-652.188) (-657.472) * [-655.570] (-649.545) (-652.536) (-657.565) -- 0:01:52 502000 -- (-660.538) [-658.073] (-656.337) (-653.853) * (-659.018) (-652.483) (-653.624) [-647.375] -- 0:01:53 503000 -- (-666.354) (-653.236) [-656.348] (-647.299) * (-651.247) (-654.251) [-654.541] (-646.927) -- 0:01:52 504000 -- [-656.863] (-653.650) (-652.108) (-665.199) * (-659.160) [-650.090] (-653.310) (-665.296) -- 0:01:52 505000 -- [-654.915] (-656.884) (-653.624) (-651.238) * [-649.059] (-656.463) (-649.964) (-649.061) -- 0:01:51 Average standard deviation of split frequencies: 0.012111 506000 -- (-653.145) [-650.079] (-652.261) (-651.959) * [-654.446] (-656.606) (-655.896) (-648.580) -- 0:01:52 507000 -- (-649.741) [-651.341] (-658.342) (-673.637) * [-645.457] (-651.855) (-651.889) (-652.686) -- 0:01:51 508000 -- [-657.490] (-665.468) (-654.420) (-652.732) * (-657.016) [-646.434] (-656.901) (-655.813) -- 0:01:51 509000 -- (-651.052) (-660.681) [-652.610] (-648.905) * [-652.824] (-653.949) (-656.237) (-659.344) -- 0:01:50 510000 -- (-657.262) (-656.728) (-655.092) [-649.731] * (-654.026) (-656.168) (-651.836) [-649.564] -- 0:01:50 Average standard deviation of split frequencies: 0.011473 511000 -- (-645.996) (-662.291) (-660.137) [-651.034] * (-650.329) (-652.760) (-652.492) [-657.254] -- 0:01:51 512000 -- (-652.573) (-655.599) [-648.475] (-662.422) * (-655.838) [-656.047] (-647.520) (-658.867) -- 0:01:50 513000 -- (-652.813) (-653.270) (-653.073) [-652.532] * (-662.981) [-646.739] (-659.747) (-650.657) -- 0:01:50 514000 -- (-657.304) (-650.733) (-664.431) [-647.484] * (-665.494) [-655.591] (-654.199) (-647.892) -- 0:01:49 515000 -- (-651.446) (-651.900) [-663.179] (-655.528) * (-671.065) (-652.950) (-647.945) [-650.318] -- 0:01:50 Average standard deviation of split frequencies: 0.011550 516000 -- [-650.710] (-655.495) (-651.805) (-654.196) * (-664.428) (-652.988) [-653.929] (-653.766) -- 0:01:49 517000 -- [-651.402] (-655.252) (-653.583) (-651.312) * (-655.366) (-657.536) (-668.254) [-653.202] -- 0:01:49 518000 -- (-655.622) [-656.380] (-652.705) (-653.364) * [-661.230] (-645.207) (-655.674) (-645.064) -- 0:01:48 519000 -- [-654.529] (-658.008) (-646.400) (-650.138) * [-653.866] (-648.734) (-654.745) (-649.923) -- 0:01:49 520000 -- [-654.419] (-656.387) (-646.565) (-658.339) * (-655.688) (-653.195) (-654.235) [-652.591] -- 0:01:48 Average standard deviation of split frequencies: 0.011188 521000 -- (-655.496) [-648.521] (-660.767) (-657.750) * [-654.863] (-649.960) (-651.835) (-653.774) -- 0:01:48 522000 -- [-652.315] (-657.931) (-644.922) (-647.458) * (-654.714) (-657.641) [-653.958] (-653.102) -- 0:01:48 523000 -- (-650.964) [-655.730] (-648.029) (-657.253) * (-650.448) (-665.623) [-656.292] (-650.581) -- 0:01:47 524000 -- (-656.103) (-651.880) [-649.404] (-652.380) * (-659.515) (-659.793) [-650.402] (-651.501) -- 0:01:48 525000 -- [-652.388] (-651.994) (-651.638) (-651.470) * (-653.290) (-669.408) (-646.249) [-650.606] -- 0:01:47 Average standard deviation of split frequencies: 0.011011 526000 -- (-658.493) (-651.833) (-653.366) [-650.013] * (-656.768) (-656.712) (-655.485) [-650.803] -- 0:01:47 527000 -- (-649.417) (-651.431) [-657.993] (-658.543) * (-654.365) (-650.446) [-650.002] (-661.318) -- 0:01:46 528000 -- [-659.576] (-654.122) (-653.011) (-652.788) * [-656.584] (-658.631) (-657.159) (-665.543) -- 0:01:47 529000 -- (-648.950) [-648.828] (-652.555) (-656.638) * [-653.667] (-655.940) (-658.384) (-659.402) -- 0:01:46 530000 -- (-658.725) [-653.154] (-651.758) (-657.070) * (-651.614) (-660.156) [-648.003] (-655.307) -- 0:01:46 Average standard deviation of split frequencies: 0.010723 531000 -- (-654.119) [-646.600] (-652.000) (-656.546) * (-657.503) (-646.465) (-651.049) [-649.604] -- 0:01:45 532000 -- (-653.978) (-652.651) (-648.560) [-654.449] * (-652.610) (-657.917) (-660.149) [-647.387] -- 0:01:46 533000 -- [-653.761] (-657.660) (-648.016) (-658.342) * (-657.929) (-647.682) [-649.891] (-654.666) -- 0:01:46 534000 -- (-657.146) [-650.206] (-646.161) (-657.407) * (-657.895) [-652.124] (-651.667) (-658.248) -- 0:01:45 535000 -- [-651.540] (-645.146) (-655.377) (-654.522) * (-648.338) (-654.403) [-650.255] (-655.955) -- 0:01:45 Average standard deviation of split frequencies: 0.010554 536000 -- (-661.155) (-655.280) [-650.158] (-647.235) * (-650.343) (-666.183) (-655.679) [-651.273] -- 0:01:44 537000 -- (-661.844) (-649.543) [-647.384] (-649.194) * (-655.234) (-663.939) [-648.649] (-650.866) -- 0:01:45 538000 -- (-665.333) (-654.615) [-650.516] (-658.025) * (-658.403) (-651.732) (-653.647) [-648.676] -- 0:01:44 539000 -- (-655.831) [-651.012] (-658.210) (-650.195) * (-657.106) (-653.468) [-655.185] (-655.241) -- 0:01:44 540000 -- (-652.354) (-648.491) (-654.108) [-652.245] * (-656.799) (-652.230) (-667.129) [-650.885] -- 0:01:43 Average standard deviation of split frequencies: 0.011023 541000 -- (-648.985) [-650.856] (-654.081) (-668.553) * (-650.738) (-655.466) (-656.459) [-654.732] -- 0:01:44 542000 -- (-654.418) [-647.876] (-650.528) (-653.158) * (-657.021) [-650.336] (-655.110) (-653.436) -- 0:01:43 543000 -- (-655.222) [-653.273] (-649.673) (-647.297) * [-647.500] (-651.460) (-656.329) (-654.610) -- 0:01:43 544000 -- [-653.155] (-644.117) (-654.410) (-653.132) * (-652.129) [-654.476] (-665.894) (-663.292) -- 0:01:43 545000 -- (-650.411) (-656.222) [-654.870] (-653.765) * (-658.066) (-646.167) (-664.742) [-644.401] -- 0:01:42 Average standard deviation of split frequencies: 0.011409 546000 -- (-653.367) (-649.871) [-659.688] (-660.712) * (-662.200) [-650.026] (-652.532) (-647.482) -- 0:01:43 547000 -- (-661.012) (-649.469) [-655.735] (-657.504) * (-647.335) (-659.383) (-658.649) [-652.648] -- 0:01:42 548000 -- (-655.623) (-648.967) (-668.980) [-652.630] * (-651.106) (-652.125) [-651.659] (-663.518) -- 0:01:42 549000 -- (-657.287) [-651.031] (-662.372) (-656.713) * (-655.858) [-647.829] (-661.392) (-659.989) -- 0:01:41 550000 -- (-665.921) (-651.677) (-652.210) [-646.782] * (-658.023) [-653.551] (-658.541) (-650.762) -- 0:01:42 Average standard deviation of split frequencies: 0.010945 551000 -- (-649.859) [-654.850] (-650.110) (-656.816) * (-654.338) [-646.567] (-664.388) (-656.697) -- 0:01:41 552000 -- [-654.502] (-650.858) (-655.473) (-660.893) * (-650.177) (-656.976) [-651.124] (-657.851) -- 0:01:41 553000 -- [-652.086] (-652.621) (-648.202) (-662.585) * [-653.439] (-664.409) (-659.072) (-651.479) -- 0:01:41 554000 -- (-656.598) [-648.008] (-644.349) (-655.760) * (-656.203) (-659.781) (-655.715) [-655.983] -- 0:01:40 555000 -- (-651.910) [-658.621] (-652.590) (-662.717) * (-656.685) (-647.643) (-660.821) [-654.889] -- 0:01:41 Average standard deviation of split frequencies: 0.010235 556000 -- (-654.116) (-656.681) (-651.950) [-650.551] * (-657.051) [-646.357] (-662.136) (-651.662) -- 0:01:40 557000 -- (-650.623) (-652.384) (-656.150) [-649.624] * (-650.380) (-657.844) [-650.729] (-653.525) -- 0:01:40 558000 -- (-655.024) [-650.609] (-654.130) (-656.833) * (-654.050) (-659.487) [-653.312] (-652.380) -- 0:01:39 559000 -- (-652.542) (-647.250) [-654.169] (-651.068) * (-648.392) (-657.976) (-655.114) [-653.362] -- 0:01:40 560000 -- (-661.153) (-656.120) [-652.776] (-658.853) * (-648.907) [-647.191] (-655.316) (-656.886) -- 0:01:39 Average standard deviation of split frequencies: 0.009489 561000 -- (-656.460) (-651.681) [-655.553] (-660.511) * (-654.584) (-653.427) [-652.434] (-652.428) -- 0:01:39 562000 -- (-656.030) (-650.567) [-649.793] (-652.057) * [-651.207] (-651.119) (-660.783) (-655.035) -- 0:01:38 563000 -- (-657.932) (-658.237) (-649.068) [-652.740] * (-656.514) (-659.404) [-657.276] (-659.422) -- 0:01:38 564000 -- (-655.877) (-654.960) (-649.939) [-645.731] * (-655.909) (-656.317) [-645.981] (-661.112) -- 0:01:38 565000 -- (-651.702) (-656.282) (-647.389) [-646.642] * (-655.070) (-646.634) (-659.709) [-651.908] -- 0:01:38 Average standard deviation of split frequencies: 0.009994 566000 -- [-651.429] (-650.061) (-648.478) (-649.092) * (-654.149) (-655.958) (-656.415) [-653.537] -- 0:01:38 567000 -- [-655.824] (-652.243) (-645.477) (-652.765) * (-658.625) (-651.311) (-649.350) [-647.110] -- 0:01:37 568000 -- (-647.118) (-650.437) [-649.966] (-648.560) * (-651.033) (-645.480) (-654.024) [-649.009] -- 0:01:38 569000 -- (-651.709) [-648.718] (-658.355) (-647.303) * (-653.965) (-665.735) (-657.026) [-646.727] -- 0:01:37 570000 -- [-649.955] (-652.861) (-651.814) (-652.143) * [-647.670] (-659.915) (-651.843) (-651.240) -- 0:01:37 Average standard deviation of split frequencies: 0.010208 571000 -- (-656.419) (-646.901) (-668.665) [-654.714] * (-654.084) [-651.128] (-648.923) (-657.789) -- 0:01:36 572000 -- (-652.032) [-647.576] (-657.855) (-654.984) * (-660.358) (-655.866) [-651.187] (-654.456) -- 0:01:36 573000 -- (-650.475) (-649.999) (-655.901) [-646.484] * (-648.914) [-650.324] (-653.973) (-651.414) -- 0:01:36 574000 -- (-660.619) (-649.288) (-654.788) [-651.001] * [-650.502] (-653.563) (-655.206) (-648.760) -- 0:01:36 575000 -- (-658.748) (-647.870) (-647.134) [-656.830] * [-647.015] (-654.257) (-654.915) (-652.525) -- 0:01:36 Average standard deviation of split frequencies: 0.009821 576000 -- (-653.405) [-651.810] (-652.662) (-647.638) * (-653.044) (-652.907) (-658.530) [-647.382] -- 0:01:35 577000 -- (-659.421) [-655.243] (-648.730) (-656.162) * (-655.822) (-653.705) [-649.105] (-653.486) -- 0:01:36 578000 -- [-651.119] (-656.357) (-657.259) (-648.042) * (-653.367) [-651.116] (-656.182) (-655.518) -- 0:01:35 579000 -- (-658.547) [-655.314] (-655.372) (-655.201) * (-662.179) (-659.593) (-653.739) [-653.723] -- 0:01:35 580000 -- (-653.205) [-651.964] (-659.910) (-654.523) * [-652.426] (-661.588) (-646.093) (-650.437) -- 0:01:34 Average standard deviation of split frequencies: 0.010032 581000 -- (-655.645) [-660.030] (-659.708) (-660.608) * (-655.304) (-660.682) [-647.163] (-654.861) -- 0:01:34 582000 -- [-654.496] (-655.198) (-668.550) (-646.740) * (-650.600) (-653.204) (-647.348) [-656.770] -- 0:01:34 583000 -- (-651.963) (-654.535) (-654.177) [-651.704] * (-647.597) (-658.565) [-651.017] (-659.123) -- 0:01:34 584000 -- (-648.577) [-654.484] (-661.818) (-656.844) * (-652.871) (-658.871) (-656.117) [-652.434] -- 0:01:34 585000 -- [-652.122] (-654.174) (-654.462) (-660.553) * (-657.173) [-649.286] (-659.506) (-653.877) -- 0:01:33 Average standard deviation of split frequencies: 0.009941 586000 -- (-648.973) (-659.359) [-648.612] (-657.851) * (-656.603) [-653.119] (-654.759) (-654.516) -- 0:01:33 587000 -- (-655.559) (-654.118) (-659.689) [-655.372] * [-648.907] (-664.872) (-654.812) (-657.905) -- 0:01:33 588000 -- (-648.058) [-654.241] (-656.153) (-652.363) * (-650.668) (-657.884) [-648.735] (-653.425) -- 0:01:33 589000 -- (-647.696) (-655.397) [-647.152] (-653.976) * (-652.723) (-655.117) [-648.556] (-652.820) -- 0:01:32 590000 -- [-648.803] (-662.777) (-656.619) (-656.214) * (-654.452) (-659.653) (-649.169) [-644.597] -- 0:01:32 Average standard deviation of split frequencies: 0.009634 591000 -- (-651.243) (-659.054) [-648.390] (-647.691) * (-656.711) (-647.680) (-653.527) [-650.742] -- 0:01:32 592000 -- (-659.129) (-666.467) (-651.347) [-655.022] * [-661.506] (-647.865) (-654.359) (-650.619) -- 0:01:32 593000 -- (-649.265) (-648.438) (-656.461) [-646.209] * (-652.149) (-658.974) (-650.783) [-658.990] -- 0:01:31 594000 -- (-652.216) (-654.593) (-649.168) [-646.732] * [-648.170] (-648.661) (-645.643) (-654.769) -- 0:01:31 595000 -- (-651.555) [-654.610] (-652.376) (-651.577) * (-655.683) (-646.508) [-647.785] (-658.038) -- 0:01:31 Average standard deviation of split frequencies: 0.009830 596000 -- (-646.549) (-660.559) (-670.575) [-651.392] * (-650.234) [-646.751] (-657.289) (-649.798) -- 0:01:31 597000 -- [-653.516] (-660.529) (-659.194) (-655.300) * [-647.509] (-651.062) (-651.632) (-655.661) -- 0:01:31 598000 -- [-651.245] (-663.774) (-653.263) (-654.728) * [-654.101] (-656.894) (-650.478) (-650.766) -- 0:01:30 599000 -- (-651.390) (-650.496) [-651.135] (-652.750) * (-650.887) (-651.968) [-656.687] (-652.875) -- 0:01:30 600000 -- (-656.898) (-651.646) (-662.740) [-654.274] * (-656.748) [-657.206] (-651.015) (-653.471) -- 0:01:30 Average standard deviation of split frequencies: 0.009418 601000 -- (-658.725) [-651.986] (-662.505) (-653.942) * (-654.163) (-658.684) (-652.708) [-653.925] -- 0:01:30 602000 -- [-651.432] (-647.934) (-653.971) (-652.912) * (-660.447) (-651.650) [-650.178] (-651.907) -- 0:01:29 603000 -- (-655.268) (-656.652) [-655.035] (-659.023) * (-667.619) (-652.930) [-646.981] (-652.998) -- 0:01:29 604000 -- (-659.918) (-658.484) (-650.225) [-652.688] * (-657.054) (-649.252) (-668.681) [-645.468] -- 0:01:29 605000 -- [-652.062] (-654.817) (-647.434) (-666.694) * (-651.377) (-650.712) [-652.282] (-659.254) -- 0:01:29 Average standard deviation of split frequencies: 0.009779 606000 -- [-661.359] (-658.265) (-659.030) (-651.666) * (-652.913) [-648.022] (-656.590) (-651.670) -- 0:01:29 607000 -- (-659.234) [-654.710] (-659.840) (-654.649) * [-651.694] (-649.343) (-654.141) (-656.367) -- 0:01:28 608000 -- (-655.436) (-650.250) (-651.082) [-650.009] * [-656.841] (-648.586) (-658.304) (-655.002) -- 0:01:28 609000 -- (-660.879) [-652.547] (-663.862) (-654.527) * (-650.312) (-654.668) [-646.256] (-654.948) -- 0:01:28 610000 -- (-650.463) (-654.182) [-650.774] (-649.256) * (-651.232) [-650.374] (-650.431) (-664.224) -- 0:01:28 Average standard deviation of split frequencies: 0.010476 611000 -- [-647.855] (-660.429) (-657.130) (-647.254) * (-650.886) (-663.960) [-661.413] (-650.707) -- 0:01:27 612000 -- (-653.618) [-651.970] (-652.985) (-657.138) * (-654.459) [-649.189] (-656.192) (-656.683) -- 0:01:27 613000 -- (-654.899) (-653.999) [-654.304] (-653.952) * (-655.118) [-652.374] (-652.959) (-660.734) -- 0:01:27 614000 -- (-647.555) [-650.970] (-658.727) (-653.681) * (-648.949) (-652.856) [-650.752] (-658.852) -- 0:01:27 615000 -- (-655.618) (-655.046) [-651.833] (-659.293) * [-652.152] (-672.819) (-654.732) (-653.980) -- 0:01:27 Average standard deviation of split frequencies: 0.010276 616000 -- (-663.301) (-657.499) (-653.070) [-659.733] * (-654.152) (-660.685) (-654.324) [-651.259] -- 0:01:26 617000 -- (-656.630) [-652.148] (-652.391) (-646.165) * (-654.504) [-657.228] (-664.838) (-658.526) -- 0:01:26 618000 -- (-655.071) [-653.764] (-653.209) (-648.007) * (-654.805) [-651.365] (-663.307) (-654.589) -- 0:01:26 619000 -- [-647.240] (-657.413) (-651.321) (-650.982) * (-660.202) [-655.841] (-651.984) (-656.062) -- 0:01:26 620000 -- (-659.484) [-649.930] (-650.812) (-652.162) * (-656.039) (-650.934) (-648.803) [-652.835] -- 0:01:25 Average standard deviation of split frequencies: 0.010308 621000 -- [-649.017] (-653.318) (-652.776) (-651.148) * [-654.574] (-651.208) (-654.468) (-653.582) -- 0:01:25 622000 -- (-657.925) [-651.380] (-652.679) (-655.246) * (-661.731) (-648.041) [-652.864] (-650.532) -- 0:01:25 623000 -- (-652.108) (-658.514) [-648.260] (-657.650) * (-656.931) (-650.434) [-651.024] (-660.204) -- 0:01:25 624000 -- (-652.716) (-648.749) [-652.998] (-650.352) * (-659.351) [-650.713] (-655.830) (-658.594) -- 0:01:24 625000 -- (-653.429) (-650.800) [-648.544] (-651.312) * (-657.172) [-656.467] (-663.052) (-655.149) -- 0:01:24 Average standard deviation of split frequencies: 0.010489 626000 -- (-648.262) (-653.634) (-654.354) [-651.482] * (-651.482) (-652.179) (-650.945) [-643.680] -- 0:01:24 627000 -- (-652.858) [-652.741] (-661.934) (-648.360) * (-650.353) [-650.615] (-653.694) (-666.083) -- 0:01:24 628000 -- (-657.844) (-650.242) [-650.283] (-653.236) * (-666.047) [-652.146] (-658.758) (-649.430) -- 0:01:24 629000 -- (-645.586) (-656.516) (-658.242) [-648.001] * (-660.645) (-650.011) (-659.061) [-653.753] -- 0:01:23 630000 -- [-655.429] (-650.187) (-654.844) (-653.212) * (-657.281) (-657.281) (-654.000) [-649.303] -- 0:01:23 Average standard deviation of split frequencies: 0.010785 631000 -- (-653.763) (-649.744) (-652.770) [-650.650] * (-654.357) [-651.625] (-655.539) (-656.672) -- 0:01:23 632000 -- (-656.007) (-662.133) [-653.451] (-654.154) * [-648.490] (-652.831) (-656.427) (-657.405) -- 0:01:23 633000 -- (-658.840) [-650.077] (-652.962) (-644.169) * (-659.456) [-660.225] (-649.114) (-664.620) -- 0:01:22 634000 -- [-648.808] (-657.972) (-655.801) (-648.273) * [-656.745] (-659.712) (-649.271) (-654.282) -- 0:01:22 635000 -- (-653.083) (-651.681) (-651.075) [-649.773] * [-653.629] (-649.129) (-658.056) (-654.479) -- 0:01:22 Average standard deviation of split frequencies: 0.010589 636000 -- (-666.730) [-653.575] (-655.512) (-656.140) * (-651.932) (-652.708) [-653.880] (-652.660) -- 0:01:22 637000 -- (-651.755) (-652.203) [-648.228] (-662.645) * (-656.354) (-654.989) (-660.393) [-655.133] -- 0:01:22 638000 -- (-650.806) (-650.223) [-648.025] (-652.404) * [-654.548] (-655.833) (-657.323) (-652.992) -- 0:01:21 639000 -- [-650.152] (-649.939) (-649.509) (-652.457) * (-650.837) (-646.280) (-665.041) [-656.156] -- 0:01:21 640000 -- (-651.353) [-652.273] (-660.268) (-660.978) * [-654.516] (-654.673) (-654.387) (-652.937) -- 0:01:21 Average standard deviation of split frequencies: 0.010091 641000 -- (-653.001) [-656.135] (-652.669) (-652.114) * (-654.721) [-658.286] (-654.516) (-655.327) -- 0:01:21 642000 -- (-653.709) (-655.718) (-651.459) [-654.318] * (-656.577) (-649.570) (-657.731) [-650.002] -- 0:01:20 643000 -- (-653.162) (-650.751) [-651.693] (-654.819) * [-648.157] (-655.837) (-650.971) (-651.092) -- 0:01:20 644000 -- (-650.102) [-659.676] (-654.523) (-651.141) * [-653.083] (-654.169) (-648.542) (-654.433) -- 0:01:20 645000 -- [-647.271] (-657.970) (-652.012) (-664.286) * (-650.262) (-662.689) (-652.455) [-646.806] -- 0:01:20 Average standard deviation of split frequencies: 0.010425 646000 -- (-664.681) (-650.560) [-651.116] (-655.888) * [-651.319] (-667.720) (-654.263) (-653.393) -- 0:01:20 647000 -- (-650.909) (-663.539) (-655.625) [-651.103] * (-653.506) (-650.988) (-655.851) [-652.926] -- 0:01:19 648000 -- (-648.700) (-660.988) (-657.588) [-650.053] * (-655.037) (-649.309) (-651.521) [-651.674] -- 0:01:19 649000 -- (-656.184) (-648.320) (-646.562) [-653.735] * (-655.435) [-648.461] (-651.027) (-654.286) -- 0:01:19 650000 -- (-651.551) [-656.111] (-657.084) (-661.281) * (-666.204) [-650.797] (-658.850) (-661.427) -- 0:01:19 Average standard deviation of split frequencies: 0.010453 651000 -- (-657.848) (-657.295) [-650.741] (-649.182) * (-652.995) (-651.671) [-653.897] (-651.375) -- 0:01:18 652000 -- [-656.241] (-655.258) (-653.451) (-649.753) * (-649.209) (-657.121) (-653.984) [-653.352] -- 0:01:18 653000 -- [-653.448] (-650.532) (-656.540) (-650.644) * [-650.049] (-647.847) (-655.155) (-663.591) -- 0:01:18 654000 -- (-658.057) [-648.454] (-656.059) (-649.433) * (-656.347) (-675.760) (-653.553) [-653.643] -- 0:01:18 655000 -- [-656.999] (-656.605) (-651.303) (-657.117) * (-648.147) (-652.790) (-654.428) [-658.951] -- 0:01:17 Average standard deviation of split frequencies: 0.010625 656000 -- (-657.721) (-656.221) (-649.546) [-646.757] * (-653.388) [-652.356] (-660.509) (-651.547) -- 0:01:17 657000 -- (-654.329) (-652.483) [-664.305] (-658.570) * (-655.577) (-653.433) (-650.482) [-653.726] -- 0:01:17 658000 -- [-650.690] (-654.168) (-662.625) (-659.087) * (-651.250) (-657.476) (-654.738) [-652.616] -- 0:01:17 659000 -- (-659.688) (-658.377) [-654.348] (-665.218) * (-649.364) (-651.571) (-656.878) [-647.948] -- 0:01:17 660000 -- [-644.378] (-651.161) (-657.371) (-651.597) * (-651.130) (-654.796) [-648.985] (-656.297) -- 0:01:16 Average standard deviation of split frequencies: 0.010040 661000 -- [-649.346] (-651.303) (-661.203) (-660.060) * (-663.558) (-657.673) (-651.081) [-660.355] -- 0:01:16 662000 -- (-656.779) (-657.465) (-656.645) [-650.802] * [-652.334] (-656.166) (-663.251) (-651.102) -- 0:01:16 663000 -- [-653.432] (-655.839) (-652.156) (-652.072) * [-652.794] (-651.773) (-657.879) (-651.824) -- 0:01:16 664000 -- [-651.117] (-663.130) (-650.586) (-654.693) * (-653.024) (-653.035) [-656.706] (-655.462) -- 0:01:15 665000 -- (-648.053) (-646.236) [-652.637] (-651.801) * (-654.931) (-661.416) [-653.367] (-649.520) -- 0:01:15 Average standard deviation of split frequencies: 0.010971 666000 -- (-656.616) [-656.522] (-652.608) (-650.483) * [-650.768] (-651.062) (-658.051) (-650.559) -- 0:01:15 667000 -- (-654.277) (-651.839) [-645.483] (-662.474) * [-651.976] (-655.549) (-667.350) (-653.077) -- 0:01:15 668000 -- (-651.713) [-651.693] (-658.854) (-653.198) * (-664.424) (-649.629) (-653.565) [-652.042] -- 0:01:15 669000 -- (-656.675) (-655.386) [-652.905] (-654.342) * (-657.388) (-656.900) (-651.789) [-655.580] -- 0:01:14 670000 -- [-647.062] (-645.394) (-658.133) (-660.433) * (-658.259) (-653.340) (-653.063) [-653.276] -- 0:01:14 Average standard deviation of split frequencies: 0.010694 671000 -- (-649.334) (-654.685) (-652.528) [-648.215] * [-647.184] (-650.215) (-657.569) (-646.913) -- 0:01:14 672000 -- [-657.524] (-651.719) (-655.384) (-651.234) * (-657.208) [-645.968] (-650.854) (-657.678) -- 0:01:14 673000 -- (-651.819) [-648.064] (-650.413) (-655.204) * (-652.851) [-651.528] (-657.613) (-653.384) -- 0:01:13 674000 -- (-663.204) (-650.156) [-644.904] (-653.531) * (-653.494) (-649.298) (-655.589) [-653.819] -- 0:01:13 675000 -- (-654.094) (-660.058) [-647.226] (-657.461) * [-652.427] (-656.871) (-659.583) (-652.989) -- 0:01:13 Average standard deviation of split frequencies: 0.011456 676000 -- (-660.254) [-653.291] (-652.298) (-651.242) * (-657.203) (-656.446) (-665.689) [-647.910] -- 0:01:13 677000 -- (-654.072) [-652.313] (-664.641) (-655.676) * (-653.167) [-644.538] (-655.834) (-653.592) -- 0:01:12 678000 -- (-651.704) (-652.361) (-653.358) [-651.765] * (-664.806) [-652.441] (-658.294) (-663.737) -- 0:01:12 679000 -- (-662.628) [-648.037] (-653.659) (-654.145) * (-665.143) (-667.104) (-662.665) [-647.164] -- 0:01:12 680000 -- [-656.994] (-657.292) (-656.652) (-659.421) * (-659.842) (-654.597) (-656.795) [-657.553] -- 0:01:12 Average standard deviation of split frequencies: 0.011675 681000 -- [-648.302] (-651.770) (-658.514) (-649.218) * (-659.049) (-655.454) [-654.486] (-650.357) -- 0:01:12 682000 -- (-654.265) [-649.763] (-651.659) (-658.745) * (-655.298) [-650.097] (-656.343) (-654.886) -- 0:01:11 683000 -- (-657.184) (-650.297) (-658.727) [-649.537] * (-659.264) [-649.021] (-663.385) (-653.527) -- 0:01:11 684000 -- (-650.489) (-658.372) [-649.466] (-650.963) * (-658.885) [-654.128] (-653.700) (-652.272) -- 0:01:11 685000 -- (-650.272) (-658.019) (-655.723) [-648.290] * (-645.291) [-653.974] (-656.733) (-649.788) -- 0:01:11 Average standard deviation of split frequencies: 0.011927 686000 -- (-656.300) (-653.689) (-657.628) [-657.521] * (-652.523) (-648.482) (-663.870) [-646.695] -- 0:01:10 687000 -- (-654.917) (-656.646) [-648.859] (-657.787) * (-648.817) [-648.519] (-656.528) (-649.572) -- 0:01:10 688000 -- (-658.946) (-653.766) [-652.686] (-649.225) * (-654.560) (-648.442) [-653.544] (-651.116) -- 0:01:10 689000 -- [-652.835] (-651.858) (-656.230) (-656.362) * (-652.194) (-650.287) (-657.219) [-646.088] -- 0:01:10 690000 -- [-646.116] (-652.503) (-651.961) (-654.882) * (-653.533) [-650.279] (-660.199) (-655.990) -- 0:01:10 Average standard deviation of split frequencies: 0.012091 691000 -- (-652.783) (-650.744) (-658.333) [-656.515] * (-664.732) (-651.401) (-650.170) [-652.758] -- 0:01:09 692000 -- (-658.785) (-650.065) (-654.148) [-655.216] * [-654.606] (-649.234) (-656.520) (-659.876) -- 0:01:09 693000 -- (-657.641) [-652.213] (-650.416) (-665.452) * (-647.880) (-665.497) [-652.725] (-647.152) -- 0:01:09 694000 -- [-650.510] (-661.624) (-659.802) (-658.166) * (-658.332) (-658.484) [-651.651] (-659.339) -- 0:01:09 695000 -- (-654.461) [-655.472] (-655.251) (-656.616) * (-651.111) [-653.172] (-651.248) (-668.731) -- 0:01:08 Average standard deviation of split frequencies: 0.011756 696000 -- (-655.939) [-650.354] (-650.706) (-651.758) * [-650.334] (-647.966) (-657.374) (-652.782) -- 0:01:08 697000 -- [-652.162] (-659.536) (-655.226) (-650.103) * [-649.329] (-646.901) (-647.759) (-657.698) -- 0:01:08 698000 -- (-654.323) [-653.660] (-654.293) (-649.561) * (-662.436) (-657.871) [-647.583] (-660.016) -- 0:01:08 699000 -- [-652.255] (-659.238) (-657.862) (-651.763) * (-650.207) [-647.311] (-655.274) (-652.109) -- 0:01:08 700000 -- [-658.784] (-645.963) (-656.045) (-649.094) * [-650.557] (-655.289) (-666.648) (-652.557) -- 0:01:07 Average standard deviation of split frequencies: 0.011438 701000 -- (-661.617) (-663.609) [-650.111] (-648.747) * (-655.532) [-654.724] (-669.146) (-659.653) -- 0:01:07 702000 -- (-659.267) [-652.094] (-661.446) (-657.657) * (-647.139) [-656.998] (-657.732) (-653.321) -- 0:01:07 703000 -- (-652.879) (-650.569) (-656.201) [-647.445] * (-650.297) [-654.978] (-653.584) (-659.678) -- 0:01:07 704000 -- (-650.838) (-647.291) (-653.989) [-653.137] * (-666.934) (-655.810) (-652.519) [-657.562] -- 0:01:06 705000 -- (-654.792) (-657.631) (-653.450) [-657.674] * (-658.123) (-664.599) (-662.074) [-644.522] -- 0:01:06 Average standard deviation of split frequencies: 0.011446 706000 -- (-653.424) (-653.274) (-665.848) [-658.559] * (-664.504) (-658.228) (-672.493) [-653.692] -- 0:01:06 707000 -- (-653.662) [-651.869] (-646.831) (-662.378) * [-652.513] (-657.363) (-651.620) (-656.644) -- 0:01:06 708000 -- [-652.265] (-645.370) (-649.346) (-653.822) * [-652.018] (-659.672) (-653.155) (-653.930) -- 0:01:05 709000 -- (-653.930) (-658.616) (-652.577) [-648.868] * (-651.049) [-652.955] (-651.160) (-655.760) -- 0:01:05 710000 -- (-647.278) (-657.407) (-651.982) [-651.120] * (-652.583) [-654.709] (-653.109) (-653.880) -- 0:01:05 Average standard deviation of split frequencies: 0.011182 711000 -- (-660.849) [-652.767] (-651.769) (-661.601) * (-663.714) (-660.137) [-651.768] (-660.160) -- 0:01:05 712000 -- [-648.763] (-653.605) (-651.878) (-647.869) * (-654.555) (-666.965) [-646.677] (-659.329) -- 0:01:05 713000 -- [-648.283] (-657.953) (-651.255) (-654.221) * (-656.390) (-653.569) [-651.233] (-657.665) -- 0:01:04 714000 -- (-655.342) [-648.024] (-656.399) (-652.385) * (-661.843) (-651.808) [-654.994] (-646.808) -- 0:01:04 715000 -- [-647.957] (-654.311) (-653.909) (-652.553) * [-649.158] (-646.818) (-649.954) (-654.946) -- 0:01:04 Average standard deviation of split frequencies: 0.010863 716000 -- [-658.148] (-664.493) (-661.327) (-648.138) * (-654.263) (-655.387) (-649.745) [-647.650] -- 0:01:04 717000 -- [-648.280] (-654.618) (-665.661) (-648.762) * (-655.096) [-657.217] (-656.721) (-650.825) -- 0:01:03 718000 -- (-654.522) (-658.807) [-659.915] (-658.511) * [-652.582] (-661.551) (-652.285) (-657.237) -- 0:01:03 719000 -- (-650.177) (-659.854) [-653.451] (-656.046) * (-651.702) (-659.876) (-651.153) [-655.976] -- 0:01:03 720000 -- (-657.266) (-658.614) [-658.744] (-649.386) * (-657.200) [-658.622] (-654.695) (-661.754) -- 0:01:03 Average standard deviation of split frequencies: 0.010746 721000 -- [-651.286] (-658.556) (-650.630) (-653.112) * (-649.962) (-656.203) [-650.867] (-648.314) -- 0:01:03 722000 -- (-656.812) (-654.805) [-654.570] (-649.860) * (-655.838) (-664.047) (-653.461) [-653.070] -- 0:01:02 723000 -- (-660.932) (-651.566) [-652.612] (-651.300) * [-643.776] (-660.674) (-654.188) (-655.455) -- 0:01:02 724000 -- (-664.149) (-654.742) [-660.105] (-658.832) * (-653.472) (-652.000) (-653.027) [-667.999] -- 0:01:02 725000 -- (-660.133) [-657.427] (-654.452) (-659.551) * (-661.319) (-661.506) [-646.914] (-656.334) -- 0:01:02 Average standard deviation of split frequencies: 0.010806 726000 -- (-657.553) (-659.712) (-652.110) [-647.427] * (-658.121) (-660.757) (-654.635) [-650.388] -- 0:01:01 727000 -- (-659.366) (-660.493) (-651.759) [-649.625] * (-659.099) (-652.348) [-655.286] (-653.453) -- 0:01:01 728000 -- [-653.883] (-661.062) (-654.627) (-656.314) * [-645.698] (-650.394) (-656.186) (-651.426) -- 0:01:01 729000 -- (-651.433) (-655.435) [-646.732] (-661.650) * [-645.942] (-669.059) (-647.212) (-664.169) -- 0:01:01 730000 -- (-659.535) (-651.032) (-650.139) [-645.539] * [-649.911] (-654.141) (-654.036) (-649.888) -- 0:01:01 Average standard deviation of split frequencies: 0.011106 731000 -- (-652.495) (-654.687) [-652.564] (-643.138) * (-649.095) [-652.981] (-651.887) (-651.086) -- 0:01:00 732000 -- (-651.262) (-660.073) [-661.406] (-657.347) * (-651.283) [-653.311] (-648.029) (-652.275) -- 0:01:00 733000 -- (-652.045) (-660.125) (-656.096) [-653.458] * (-654.095) (-662.425) (-653.188) [-651.984] -- 0:01:00 734000 -- (-649.982) [-649.187] (-650.532) (-660.898) * (-661.980) [-655.284] (-658.211) (-661.288) -- 0:01:00 735000 -- (-656.594) (-658.583) (-651.605) [-644.579] * (-656.165) (-661.495) [-649.710] (-654.924) -- 0:00:59 Average standard deviation of split frequencies: 0.010568 736000 -- (-647.037) (-653.767) (-660.204) [-650.626] * [-652.115] (-661.102) (-648.058) (-655.374) -- 0:00:59 737000 -- (-656.635) (-658.396) (-656.949) [-647.133] * [-653.029] (-656.847) (-652.736) (-648.577) -- 0:00:59 738000 -- (-651.059) (-651.292) [-654.949] (-661.219) * (-652.833) (-651.219) [-654.224] (-649.712) -- 0:00:59 739000 -- (-656.955) [-655.778] (-656.075) (-657.365) * (-659.000) [-651.990] (-651.070) (-654.244) -- 0:00:58 740000 -- [-646.540] (-648.063) (-650.651) (-656.844) * [-651.568] (-654.367) (-657.284) (-655.060) -- 0:00:59 Average standard deviation of split frequencies: 0.010593 741000 -- (-652.457) [-648.464] (-656.882) (-657.666) * (-654.040) (-656.474) (-652.973) [-652.385] -- 0:00:58 742000 -- [-661.524] (-651.487) (-653.303) (-654.938) * (-649.420) [-651.876] (-664.629) (-657.272) -- 0:00:58 743000 -- (-665.047) [-646.274] (-659.506) (-653.908) * (-659.006) [-648.781] (-661.597) (-657.278) -- 0:00:58 744000 -- (-649.144) (-653.154) [-654.170] (-659.156) * (-649.296) [-648.044] (-656.954) (-653.574) -- 0:00:58 745000 -- (-651.992) (-657.034) [-650.225] (-648.348) * [-651.886] (-651.281) (-654.415) (-657.787) -- 0:00:57 Average standard deviation of split frequencies: 0.010065 746000 -- [-649.758] (-647.970) (-658.698) (-654.152) * (-658.679) (-650.284) [-657.106] (-658.852) -- 0:00:57 747000 -- (-658.864) [-653.903] (-659.865) (-650.841) * [-649.308] (-650.745) (-661.718) (-652.000) -- 0:00:57 748000 -- [-654.102] (-657.281) (-650.375) (-657.528) * (-651.629) (-657.391) (-657.144) [-648.333] -- 0:00:56 749000 -- (-650.779) (-654.321) [-652.085] (-646.941) * [-650.638] (-661.606) (-654.687) (-652.098) -- 0:00:56 750000 -- [-653.144] (-655.215) (-654.091) (-651.892) * (-660.005) (-658.366) [-653.925] (-648.318) -- 0:00:56 Average standard deviation of split frequencies: 0.010496 751000 -- (-653.523) (-657.903) [-648.334] (-652.354) * (-655.758) [-654.372] (-651.304) (-649.695) -- 0:00:56 752000 -- (-657.741) (-654.505) [-655.827] (-648.885) * (-650.709) (-662.917) (-654.234) [-645.956] -- 0:00:56 753000 -- (-650.129) [-651.335] (-653.973) (-651.518) * [-652.504] (-653.881) (-653.084) (-645.745) -- 0:00:56 754000 -- (-654.726) (-663.188) [-653.728] (-652.687) * (-653.438) [-653.770] (-656.227) (-657.205) -- 0:00:55 755000 -- (-656.248) [-649.921] (-652.720) (-658.563) * (-657.243) (-658.261) [-654.861] (-651.434) -- 0:00:55 Average standard deviation of split frequencies: 0.010066 756000 -- (-650.733) (-656.152) [-652.346] (-653.286) * (-656.628) [-651.223] (-646.725) (-656.637) -- 0:00:55 757000 -- (-650.410) (-656.239) (-653.403) [-661.572] * (-656.945) (-655.849) [-653.399] (-652.302) -- 0:00:55 758000 -- [-647.876] (-669.013) (-655.044) (-649.552) * (-649.764) (-657.790) [-651.994] (-656.047) -- 0:00:54 759000 -- (-647.647) (-660.472) (-662.390) [-653.768] * [-650.686] (-650.478) (-657.476) (-650.982) -- 0:00:54 760000 -- (-651.905) (-648.006) (-652.236) [-651.863] * [-657.599] (-662.341) (-652.844) (-653.695) -- 0:00:54 Average standard deviation of split frequencies: 0.010624 761000 -- (-646.617) (-651.178) [-654.554] (-663.346) * (-666.325) [-650.979] (-657.059) (-650.042) -- 0:00:54 762000 -- (-659.223) (-658.887) (-654.183) [-657.601] * (-657.129) (-660.616) (-657.213) [-650.286] -- 0:00:54 763000 -- (-660.048) (-651.367) [-652.089] (-664.094) * (-653.959) (-653.776) [-652.292] (-653.612) -- 0:00:53 764000 -- (-655.868) (-646.474) (-657.718) [-650.676] * (-655.255) (-655.620) [-652.220] (-655.077) -- 0:00:53 765000 -- (-663.087) [-649.922] (-658.274) (-651.880) * (-648.047) (-653.744) (-654.980) [-645.769] -- 0:00:53 Average standard deviation of split frequencies: 0.010858 766000 -- (-653.971) (-665.131) [-646.161] (-651.600) * [-651.372] (-648.940) (-649.424) (-670.142) -- 0:00:53 767000 -- (-659.259) [-648.454] (-652.163) (-661.620) * (-651.182) (-650.657) [-649.233] (-661.122) -- 0:00:52 768000 -- (-658.024) (-644.558) [-653.210] (-655.044) * (-655.507) [-652.995] (-655.561) (-658.076) -- 0:00:52 769000 -- (-651.165) (-663.553) (-656.829) [-649.705] * (-662.731) (-660.878) [-653.042] (-651.857) -- 0:00:52 770000 -- (-658.507) [-650.728] (-652.722) (-656.548) * (-653.812) [-653.607] (-649.152) (-656.578) -- 0:00:51 Average standard deviation of split frequencies: 0.010617 771000 -- (-649.717) [-654.743] (-661.683) (-655.023) * (-653.824) (-651.276) (-652.571) [-655.239] -- 0:00:51 772000 -- (-649.256) (-660.235) [-647.903] (-651.162) * (-646.581) (-652.195) (-656.350) [-650.036] -- 0:00:51 773000 -- [-653.062] (-657.037) (-660.326) (-651.433) * (-653.917) [-649.841] (-655.915) (-648.382) -- 0:00:51 774000 -- (-647.157) (-654.523) (-652.184) [-653.493] * (-651.410) (-648.511) [-658.791] (-652.340) -- 0:00:51 775000 -- (-647.449) [-654.339] (-659.924) (-661.402) * (-656.374) (-653.141) (-650.746) [-646.915] -- 0:00:51 Average standard deviation of split frequencies: 0.010501 776000 -- [-649.036] (-657.933) (-655.722) (-652.742) * (-659.209) (-656.105) (-652.623) [-652.779] -- 0:00:50 777000 -- (-653.312) (-652.209) (-657.562) [-648.982] * (-649.380) (-656.818) [-649.123] (-657.879) -- 0:00:50 778000 -- (-657.182) [-649.709] (-653.765) (-655.901) * (-657.032) [-655.265] (-650.993) (-659.022) -- 0:00:50 779000 -- (-655.034) (-657.858) [-651.728] (-660.984) * [-647.968] (-651.791) (-660.248) (-667.678) -- 0:00:49 780000 -- (-657.078) (-661.256) [-647.070] (-652.543) * [-649.552] (-645.608) (-656.392) (-656.030) -- 0:00:49 Average standard deviation of split frequencies: 0.010179 781000 -- (-653.964) (-653.768) [-652.619] (-661.905) * (-651.679) [-659.311] (-653.401) (-665.039) -- 0:00:49 782000 -- (-661.199) [-650.894] (-660.760) (-655.443) * (-653.947) [-651.755] (-651.852) (-663.629) -- 0:00:49 783000 -- (-656.774) (-649.384) [-649.769] (-660.009) * (-654.016) (-657.375) [-648.402] (-659.937) -- 0:00:49 784000 -- (-655.567) (-653.920) (-650.682) [-647.974] * (-654.248) (-658.959) (-657.507) [-658.881] -- 0:00:49 785000 -- (-652.507) (-653.246) (-650.914) [-656.927] * (-654.721) (-654.871) [-652.802] (-655.059) -- 0:00:48 Average standard deviation of split frequencies: 0.009725 786000 -- (-652.857) (-655.267) [-659.494] (-663.737) * (-654.121) (-650.786) (-656.194) [-653.531] -- 0:00:48 787000 -- (-657.244) (-660.527) [-649.887] (-651.919) * [-647.464] (-651.554) (-655.049) (-652.707) -- 0:00:48 788000 -- [-656.801] (-656.092) (-655.316) (-653.566) * (-653.504) (-649.719) (-663.617) [-650.400] -- 0:00:48 789000 -- (-654.971) (-652.508) [-650.718] (-656.453) * [-651.292] (-654.397) (-652.638) (-659.302) -- 0:00:47 790000 -- (-649.383) [-653.552] (-655.246) (-658.633) * (-651.769) [-651.572] (-653.746) (-655.489) -- 0:00:47 Average standard deviation of split frequencies: 0.009454 791000 -- [-652.667] (-650.828) (-651.493) (-656.146) * (-656.990) (-656.763) (-650.844) [-653.870] -- 0:00:47 792000 -- (-654.086) (-655.659) [-652.226] (-656.518) * (-654.796) (-648.901) [-656.381] (-654.465) -- 0:00:47 793000 -- (-658.208) (-656.388) (-663.455) [-648.601] * (-659.995) (-651.703) (-652.489) [-649.612] -- 0:00:46 794000 -- (-652.451) (-661.845) [-647.684] (-646.544) * (-647.230) [-664.630] (-650.809) (-657.339) -- 0:00:46 795000 -- (-645.569) [-647.589] (-656.391) (-666.831) * [-652.983] (-647.696) (-659.718) (-650.272) -- 0:00:46 Average standard deviation of split frequencies: 0.009391 796000 -- (-650.673) (-654.131) [-653.722] (-646.724) * (-654.943) (-660.637) [-651.699] (-650.764) -- 0:00:46 797000 -- [-655.279] (-654.469) (-650.868) (-652.099) * (-657.546) (-651.972) [-654.499] (-655.873) -- 0:00:46 798000 -- (-649.205) [-651.809] (-655.198) (-649.933) * (-664.954) (-652.340) (-655.046) [-658.094] -- 0:00:45 799000 -- (-650.723) (-656.280) (-658.238) [-655.119] * [-660.007] (-654.538) (-655.745) (-648.970) -- 0:00:45 800000 -- (-656.495) (-647.410) (-654.791) [-648.729] * (-649.028) (-650.985) [-653.865] (-664.871) -- 0:00:45 Average standard deviation of split frequencies: 0.009084 801000 -- (-657.797) (-659.585) [-651.624] (-665.506) * (-652.672) (-652.782) [-649.235] (-651.907) -- 0:00:45 802000 -- (-655.473) (-652.059) [-646.761] (-659.236) * [-653.437] (-653.379) (-648.094) (-651.431) -- 0:00:44 803000 -- (-669.149) (-653.801) [-657.026] (-659.199) * (-653.703) (-649.429) (-661.855) [-650.513] -- 0:00:44 804000 -- (-665.865) [-656.194] (-658.638) (-652.518) * (-649.294) (-651.453) (-662.836) [-660.915] -- 0:00:44 805000 -- [-655.697] (-656.075) (-667.177) (-649.086) * [-649.093] (-650.072) (-651.983) (-657.177) -- 0:00:44 Average standard deviation of split frequencies: 0.008898 806000 -- (-659.926) [-651.102] (-659.383) (-653.170) * (-662.987) (-655.392) [-655.038] (-651.798) -- 0:00:44 807000 -- (-667.537) (-655.254) [-654.314] (-649.027) * (-648.878) [-651.046] (-651.019) (-653.669) -- 0:00:43 808000 -- (-656.944) (-649.497) [-657.241] (-651.933) * [-651.331] (-650.077) (-654.712) (-656.922) -- 0:00:43 809000 -- [-654.233] (-658.675) (-653.110) (-644.470) * (-660.140) (-659.880) (-654.712) [-651.128] -- 0:00:43 810000 -- (-659.331) [-655.387] (-656.552) (-652.670) * (-652.394) [-648.681] (-650.303) (-661.937) -- 0:00:43 Average standard deviation of split frequencies: 0.009096 811000 -- (-660.599) (-655.018) [-647.797] (-652.432) * (-653.536) (-652.021) (-658.935) [-657.116] -- 0:00:42 812000 -- (-655.843) [-656.484] (-652.386) (-658.167) * (-659.804) [-655.519] (-661.977) (-655.124) -- 0:00:42 813000 -- (-652.715) (-658.809) (-651.113) [-649.374] * (-658.755) (-655.053) [-654.721] (-652.573) -- 0:00:42 814000 -- [-647.521] (-659.635) (-648.386) (-649.066) * (-657.441) (-661.331) [-654.853] (-647.926) -- 0:00:42 815000 -- (-653.702) [-652.769] (-656.238) (-656.493) * (-656.745) [-657.275] (-653.919) (-656.032) -- 0:00:41 Average standard deviation of split frequencies: 0.008996 816000 -- (-651.640) [-647.421] (-663.479) (-657.659) * [-649.179] (-653.324) (-665.289) (-658.483) -- 0:00:41 817000 -- (-665.528) (-653.379) [-652.105] (-656.736) * (-663.177) (-652.802) (-654.104) [-645.422] -- 0:00:41 818000 -- (-652.059) [-651.778] (-651.601) (-660.787) * [-650.434] (-658.651) (-652.213) (-660.524) -- 0:00:41 819000 -- (-655.651) [-649.677] (-655.788) (-650.398) * (-658.243) [-650.824] (-655.530) (-663.358) -- 0:00:41 820000 -- [-648.187] (-651.780) (-656.564) (-654.028) * (-659.967) [-645.576] (-657.320) (-649.447) -- 0:00:40 Average standard deviation of split frequencies: 0.008739 821000 -- (-650.915) [-653.174] (-656.051) (-659.658) * (-648.517) (-658.260) (-656.425) [-654.283] -- 0:00:40 822000 -- (-651.837) (-653.399) [-649.229] (-653.767) * (-650.325) (-658.110) [-650.912] (-652.971) -- 0:00:40 823000 -- (-659.379) [-659.790] (-653.995) (-653.095) * (-653.613) (-649.696) (-649.557) [-646.474] -- 0:00:40 824000 -- (-648.454) [-649.074] (-653.055) (-653.571) * (-649.927) (-650.585) (-657.439) [-650.219] -- 0:00:39 825000 -- [-656.265] (-654.056) (-646.782) (-653.088) * (-656.884) [-654.869] (-656.781) (-649.694) -- 0:00:39 Average standard deviation of split frequencies: 0.008683 826000 -- (-647.430) (-651.664) (-651.391) [-654.337] * (-649.672) (-657.329) [-648.412] (-649.302) -- 0:00:39 827000 -- (-655.752) (-653.167) [-651.782] (-656.260) * (-664.381) (-659.667) [-653.767] (-658.747) -- 0:00:39 828000 -- (-649.770) (-652.151) [-649.924] (-656.775) * [-652.706] (-648.748) (-655.945) (-650.127) -- 0:00:39 829000 -- [-652.338] (-649.652) (-655.069) (-656.102) * (-652.757) [-650.694] (-654.680) (-649.932) -- 0:00:38 830000 -- [-647.085] (-657.825) (-655.628) (-657.569) * (-651.435) (-650.578) [-655.112] (-651.857) -- 0:00:38 Average standard deviation of split frequencies: 0.008958 831000 -- [-648.048] (-650.823) (-658.002) (-646.620) * (-658.125) (-657.907) [-655.938] (-654.285) -- 0:00:38 832000 -- [-652.299] (-648.621) (-657.901) (-652.559) * (-648.014) (-652.168) [-650.935] (-658.173) -- 0:00:38 833000 -- [-656.947] (-654.937) (-651.723) (-657.689) * [-652.631] (-651.092) (-649.140) (-658.681) -- 0:00:37 834000 -- [-649.076] (-657.186) (-650.976) (-657.823) * (-648.909) [-650.507] (-655.239) (-659.357) -- 0:00:37 835000 -- (-653.847) [-652.500] (-647.692) (-658.500) * (-660.850) (-659.701) [-651.442] (-661.892) -- 0:00:37 Average standard deviation of split frequencies: 0.008498 836000 -- (-658.914) (-658.043) [-656.018] (-654.188) * [-651.730] (-653.421) (-661.189) (-653.232) -- 0:00:37 837000 -- (-655.040) (-655.499) [-653.510] (-651.820) * (-651.829) (-662.757) (-659.385) [-650.771] -- 0:00:37 838000 -- (-657.611) (-656.980) [-652.127] (-652.438) * (-659.411) [-653.465] (-656.220) (-653.034) -- 0:00:36 839000 -- (-654.497) (-656.799) (-659.455) [-650.506] * (-658.105) (-657.733) (-660.568) [-649.987] -- 0:00:36 840000 -- (-653.945) (-653.327) (-653.293) [-650.787] * (-656.491) (-655.719) (-653.378) [-647.308] -- 0:00:36 Average standard deviation of split frequencies: 0.008411 841000 -- [-655.387] (-652.039) (-652.679) (-655.296) * [-650.667] (-647.421) (-647.612) (-646.616) -- 0:00:36 842000 -- (-653.019) [-654.468] (-651.303) (-653.309) * [-653.851] (-655.218) (-660.271) (-658.850) -- 0:00:35 843000 -- (-650.673) (-653.880) [-648.859] (-661.761) * (-655.816) [-650.524] (-659.285) (-663.485) -- 0:00:35 844000 -- (-648.821) (-653.508) (-651.574) [-652.974] * [-652.751] (-652.367) (-670.628) (-658.873) -- 0:00:35 845000 -- [-650.738] (-649.646) (-659.398) (-655.382) * [-659.712] (-654.322) (-660.312) (-653.505) -- 0:00:35 Average standard deviation of split frequencies: 0.008358 846000 -- (-652.901) [-652.478] (-655.408) (-649.953) * (-667.649) [-647.380] (-666.912) (-650.244) -- 0:00:34 847000 -- [-650.392] (-657.781) (-651.815) (-652.167) * (-653.232) [-653.343] (-655.443) (-652.734) -- 0:00:34 848000 -- [-644.369] (-650.542) (-657.059) (-654.530) * (-653.482) (-652.169) (-654.292) [-649.430] -- 0:00:34 849000 -- (-657.136) (-653.779) (-662.543) [-648.986] * (-652.839) [-656.125] (-652.594) (-651.927) -- 0:00:34 850000 -- (-660.311) [-645.431] (-661.898) (-659.554) * (-657.484) (-654.262) (-655.131) [-651.021] -- 0:00:34 Average standard deviation of split frequencies: 0.008748 851000 -- (-655.672) (-648.709) (-652.712) [-655.343] * (-648.423) [-650.063] (-653.589) (-659.110) -- 0:00:33 852000 -- (-656.260) [-651.911] (-646.264) (-652.206) * (-657.381) [-653.230] (-657.590) (-658.706) -- 0:00:33 853000 -- (-656.270) (-649.324) [-654.433] (-655.869) * (-655.653) [-650.882] (-652.355) (-652.904) -- 0:00:33 854000 -- (-657.559) (-650.174) (-653.260) [-649.013] * (-652.459) [-651.106] (-650.514) (-659.395) -- 0:00:33 855000 -- (-651.358) (-645.035) (-659.101) [-651.802] * (-650.216) [-648.216] (-663.360) (-653.695) -- 0:00:32 Average standard deviation of split frequencies: 0.009205 856000 -- (-651.241) [-655.535] (-656.731) (-655.332) * (-654.349) (-655.708) (-654.111) [-652.125] -- 0:00:32 857000 -- [-646.425] (-656.822) (-654.115) (-655.862) * (-660.224) [-648.927] (-653.605) (-653.283) -- 0:00:32 858000 -- [-649.914] (-653.843) (-655.232) (-646.226) * (-645.617) (-658.259) (-659.325) [-655.230] -- 0:00:32 859000 -- (-654.525) [-653.269] (-656.199) (-650.408) * [-651.275] (-669.104) (-650.542) (-656.166) -- 0:00:32 860000 -- (-656.230) (-651.812) (-654.461) [-654.182] * (-653.495) [-652.721] (-657.484) (-655.417) -- 0:00:31 Average standard deviation of split frequencies: 0.009702 861000 -- (-653.081) (-657.558) [-651.484] (-656.526) * (-656.471) [-654.655] (-653.816) (-652.284) -- 0:00:31 862000 -- [-658.693] (-662.420) (-655.137) (-654.358) * (-656.252) (-654.227) [-646.665] (-650.992) -- 0:00:31 863000 -- (-653.487) (-657.660) [-654.703] (-657.522) * [-654.859] (-663.179) (-650.223) (-654.833) -- 0:00:31 864000 -- (-649.588) [-650.775] (-652.957) (-651.425) * (-655.430) [-654.006] (-651.578) (-652.380) -- 0:00:30 865000 -- (-664.163) [-648.328] (-649.283) (-655.051) * (-653.393) [-659.578] (-649.307) (-652.259) -- 0:00:30 Average standard deviation of split frequencies: 0.009643 866000 -- (-654.823) (-656.297) (-658.106) [-650.777] * (-657.675) (-665.337) (-654.252) [-654.599] -- 0:00:30 867000 -- (-651.609) (-660.864) (-648.907) [-652.870] * (-660.864) (-654.840) [-649.762] (-655.655) -- 0:00:30 868000 -- (-649.799) (-654.708) [-653.266] (-660.846) * (-658.458) (-652.371) [-653.129] (-653.107) -- 0:00:29 869000 -- (-654.877) (-658.640) (-653.592) [-657.050] * [-651.167] (-661.305) (-654.656) (-646.882) -- 0:00:29 870000 -- (-644.853) (-651.383) (-649.181) [-653.251] * (-652.726) [-650.394] (-649.387) (-656.415) -- 0:00:29 Average standard deviation of split frequencies: 0.009475 871000 -- (-656.831) [-650.841] (-656.738) (-655.261) * (-655.651) [-649.000] (-660.082) (-649.098) -- 0:00:29 872000 -- (-655.249) (-659.556) (-647.511) [-646.627] * (-657.319) (-647.531) (-649.470) [-648.165] -- 0:00:29 873000 -- (-651.098) (-651.246) [-656.489] (-651.086) * (-650.544) (-661.681) [-649.088] (-654.462) -- 0:00:28 874000 -- [-650.367] (-647.082) (-657.418) (-657.747) * (-654.014) [-653.014] (-659.980) (-658.309) -- 0:00:28 875000 -- (-654.307) (-663.735) (-663.032) [-651.534] * [-648.035] (-670.681) (-652.297) (-650.351) -- 0:00:28 Average standard deviation of split frequencies: 0.008879 876000 -- (-653.030) [-654.162] (-666.732) (-648.694) * [-657.789] (-660.284) (-657.469) (-653.604) -- 0:00:28 877000 -- (-658.378) (-655.457) (-655.011) [-653.412] * (-648.562) (-656.522) (-654.434) [-648.216] -- 0:00:27 878000 -- (-654.179) [-646.880] (-655.332) (-656.947) * (-655.721) (-657.004) (-656.329) [-657.020] -- 0:00:27 879000 -- (-652.396) (-655.614) (-660.803) [-654.191] * [-647.183] (-654.762) (-650.704) (-655.998) -- 0:00:27 880000 -- [-651.311] (-651.127) (-662.776) (-651.437) * (-648.056) [-652.317] (-655.706) (-666.816) -- 0:00:27 Average standard deviation of split frequencies: 0.008603 881000 -- [-651.283] (-651.332) (-664.294) (-656.236) * (-658.075) [-649.895] (-655.061) (-657.226) -- 0:00:27 882000 -- (-651.309) [-652.164] (-654.756) (-654.457) * (-656.992) [-655.274] (-650.227) (-659.306) -- 0:00:26 883000 -- (-660.162) (-656.274) (-660.747) [-655.369] * (-656.577) (-656.317) [-657.695] (-651.450) -- 0:00:26 884000 -- (-651.692) [-650.755] (-649.301) (-659.193) * [-655.449] (-654.492) (-657.781) (-653.355) -- 0:00:26 885000 -- [-659.596] (-654.554) (-654.297) (-652.110) * [-660.042] (-647.235) (-660.685) (-652.747) -- 0:00:26 Average standard deviation of split frequencies: 0.008437 886000 -- (-653.318) [-647.489] (-658.932) (-652.938) * [-647.237] (-655.841) (-650.798) (-647.982) -- 0:00:25 887000 -- (-655.600) (-656.834) (-654.127) [-660.674] * (-656.940) (-658.272) (-652.552) [-654.428] -- 0:00:25 888000 -- (-650.925) (-656.625) [-654.959] (-650.589) * (-650.626) (-648.779) [-651.621] (-655.271) -- 0:00:25 889000 -- (-656.718) (-659.861) [-650.179] (-647.001) * (-653.184) (-659.676) [-652.421] (-649.942) -- 0:00:25 890000 -- (-654.959) (-655.695) [-650.956] (-645.029) * (-657.045) (-653.665) [-648.042] (-652.195) -- 0:00:24 Average standard deviation of split frequencies: 0.007788 891000 -- (-654.764) (-651.859) (-648.535) [-649.657] * [-654.342] (-656.361) (-657.524) (-649.475) -- 0:00:24 892000 -- [-652.379] (-653.571) (-650.984) (-659.957) * (-666.006) (-649.833) (-658.186) [-651.447] -- 0:00:24 893000 -- (-663.497) (-650.167) (-650.364) [-655.980] * [-651.495] (-652.492) (-656.063) (-651.117) -- 0:00:24 894000 -- [-655.991] (-659.194) (-659.687) (-659.923) * [-651.160] (-654.795) (-657.910) (-652.958) -- 0:00:24 895000 -- (-654.683) (-653.164) (-652.446) [-657.779] * [-656.331] (-645.668) (-653.066) (-655.336) -- 0:00:23 Average standard deviation of split frequencies: 0.007704 896000 -- (-659.775) (-652.904) (-654.480) [-653.530] * (-659.843) (-658.701) (-652.952) [-651.751] -- 0:00:23 897000 -- (-649.042) (-653.562) [-648.720] (-659.057) * (-655.652) (-653.221) (-658.502) [-656.963] -- 0:00:23 898000 -- (-658.639) (-655.201) [-652.321] (-652.429) * (-652.566) [-649.626] (-652.396) (-655.220) -- 0:00:23 899000 -- (-647.865) [-649.784] (-658.609) (-657.392) * (-654.251) [-654.748] (-659.948) (-656.694) -- 0:00:22 900000 -- (-651.235) (-658.862) [-651.012] (-659.756) * [-650.324] (-658.769) (-658.344) (-659.295) -- 0:00:22 Average standard deviation of split frequencies: 0.008000 901000 -- (-646.746) (-653.758) [-655.080] (-654.711) * (-655.036) (-647.941) [-654.322] (-652.015) -- 0:00:22 902000 -- (-651.699) (-658.268) (-650.762) [-650.634] * (-655.215) [-650.944] (-661.276) (-648.228) -- 0:00:22 903000 -- (-649.681) (-659.911) (-644.673) [-657.091] * [-661.701] (-650.552) (-665.844) (-657.856) -- 0:00:22 904000 -- (-651.714) (-652.318) (-653.437) [-653.795] * (-656.385) (-654.089) (-654.881) [-650.347] -- 0:00:21 905000 -- (-647.914) (-655.404) (-656.789) [-652.662] * (-655.882) (-655.327) (-659.959) [-656.535] -- 0:00:21 Average standard deviation of split frequencies: 0.007879 906000 -- [-650.953] (-654.122) (-664.663) (-659.460) * [-655.547] (-656.004) (-651.531) (-658.750) -- 0:00:21 907000 -- [-658.381] (-655.316) (-658.788) (-660.275) * [-652.585] (-655.582) (-655.070) (-649.433) -- 0:00:21 908000 -- (-660.823) (-657.636) (-658.395) [-651.332] * (-654.509) (-654.498) [-651.536] (-659.492) -- 0:00:20 909000 -- (-661.921) (-654.621) [-644.922] (-656.174) * (-658.633) [-657.438] (-652.495) (-657.375) -- 0:00:20 910000 -- (-657.300) (-650.564) [-648.348] (-653.902) * (-661.601) (-649.799) [-659.971] (-657.545) -- 0:00:20 Average standard deviation of split frequencies: 0.007543 911000 -- (-656.402) (-650.351) (-660.059) [-654.430] * (-656.856) (-652.664) (-650.626) [-652.983] -- 0:00:20 912000 -- (-664.501) [-649.605] (-651.420) (-655.090) * (-658.824) (-653.812) (-661.093) [-646.577] -- 0:00:19 913000 -- (-647.140) [-651.504] (-651.235) (-647.801) * (-653.655) (-647.512) (-654.781) [-657.851] -- 0:00:19 914000 -- (-664.371) [-648.827] (-654.061) (-645.579) * (-654.788) (-648.685) (-652.128) [-662.963] -- 0:00:19 915000 -- (-656.516) (-659.412) [-654.961] (-656.209) * (-654.553) (-662.608) (-649.865) [-654.341] -- 0:00:19 Average standard deviation of split frequencies: 0.007205 916000 -- (-661.012) (-653.456) [-650.091] (-663.277) * (-659.229) (-659.860) [-650.911] (-649.330) -- 0:00:19 917000 -- (-652.638) [-652.202] (-654.763) (-652.831) * (-646.765) (-657.494) [-647.253] (-654.503) -- 0:00:18 918000 -- (-658.028) (-658.867) (-654.085) [-653.784] * (-647.460) [-650.066] (-651.101) (-657.761) -- 0:00:18 919000 -- (-651.829) (-658.314) (-657.377) [-651.820] * [-647.989] (-651.325) (-653.837) (-657.286) -- 0:00:18 920000 -- (-657.037) (-648.838) (-662.336) [-659.883] * (-651.508) (-654.956) (-651.388) [-653.239] -- 0:00:18 Average standard deviation of split frequencies: 0.007424 921000 -- [-644.493] (-654.286) (-652.111) (-647.976) * (-651.023) [-651.132] (-656.510) (-655.442) -- 0:00:17 922000 -- (-657.068) [-653.939] (-652.097) (-649.562) * (-652.078) (-659.358) [-652.692] (-656.895) -- 0:00:17 923000 -- (-645.906) (-650.284) (-658.536) [-654.301] * (-654.090) (-655.250) (-656.738) [-654.871] -- 0:00:17 924000 -- [-648.871] (-651.343) (-655.193) (-649.597) * [-647.974] (-659.701) (-650.508) (-655.866) -- 0:00:17 925000 -- (-652.068) (-663.446) (-655.515) [-649.496] * (-648.693) (-663.144) (-656.111) [-652.937] -- 0:00:17 Average standard deviation of split frequencies: 0.007236 926000 -- [-650.444] (-648.465) (-651.776) (-651.107) * [-654.041] (-651.168) (-653.013) (-653.221) -- 0:00:16 927000 -- (-653.535) (-657.752) [-651.165] (-645.486) * (-661.738) (-661.307) (-655.872) [-650.494] -- 0:00:16 928000 -- (-667.512) (-666.853) (-648.591) [-650.076] * (-652.305) (-664.264) (-654.798) [-651.082] -- 0:00:16 929000 -- (-655.081) (-663.835) (-661.261) [-653.655] * (-653.388) [-648.045] (-660.850) (-653.140) -- 0:00:16 930000 -- [-651.179] (-658.989) (-655.170) (-651.203) * (-653.267) (-649.878) [-648.679] (-658.935) -- 0:00:15 Average standard deviation of split frequencies: 0.007525 931000 -- (-657.221) (-664.604) [-652.366] (-654.274) * (-653.020) (-654.856) (-652.748) [-651.233] -- 0:00:15 932000 -- (-652.958) [-649.970] (-650.175) (-655.711) * (-654.879) [-650.666] (-652.600) (-655.527) -- 0:00:15 933000 -- (-654.361) (-654.133) [-652.387] (-657.266) * (-658.158) [-647.306] (-660.037) (-654.163) -- 0:00:15 934000 -- (-660.174) [-657.919] (-651.343) (-657.106) * (-652.077) (-662.008) [-653.004] (-650.575) -- 0:00:14 935000 -- [-657.895] (-651.071) (-653.450) (-658.095) * (-655.682) (-649.409) (-650.643) [-647.174] -- 0:00:14 Average standard deviation of split frequencies: 0.007878 936000 -- [-655.004] (-651.628) (-650.907) (-655.540) * (-653.569) [-649.331] (-647.005) (-652.450) -- 0:00:14 937000 -- [-646.688] (-646.007) (-660.725) (-647.273) * [-647.833] (-652.723) (-658.938) (-653.110) -- 0:00:14 938000 -- (-671.617) (-646.164) [-654.749] (-652.165) * (-651.466) [-651.562] (-655.898) (-649.987) -- 0:00:14 939000 -- (-659.554) (-666.136) [-650.177] (-659.240) * (-656.622) (-648.533) [-648.590] (-648.013) -- 0:00:13 940000 -- (-658.418) (-655.123) [-647.057] (-664.543) * [-648.100] (-647.561) (-654.005) (-664.548) -- 0:00:13 Average standard deviation of split frequencies: 0.007947 941000 -- (-657.575) [-648.685] (-647.698) (-659.454) * (-653.284) (-655.071) [-655.358] (-662.819) -- 0:00:13 942000 -- [-656.967] (-657.430) (-652.422) (-652.987) * (-659.197) [-650.801] (-658.028) (-655.259) -- 0:00:13 943000 -- (-657.695) [-650.709] (-648.468) (-659.855) * (-652.343) (-651.034) (-653.742) [-647.893] -- 0:00:12 944000 -- (-652.602) [-653.142] (-660.368) (-649.768) * (-655.709) (-655.404) [-648.941] (-652.155) -- 0:00:12 945000 -- (-650.293) (-650.555) [-653.494] (-658.532) * (-667.397) (-659.994) (-653.127) [-655.421] -- 0:00:12 Average standard deviation of split frequencies: 0.007937 946000 -- (-658.380) (-652.077) (-655.797) [-648.560] * [-656.478] (-656.988) (-655.437) (-660.427) -- 0:00:12 947000 -- (-659.992) (-655.339) (-655.044) [-650.666] * (-653.873) (-652.783) [-650.781] (-650.466) -- 0:00:12 948000 -- (-655.094) (-658.142) [-656.403] (-661.211) * [-656.395] (-650.481) (-660.723) (-655.683) -- 0:00:11 949000 -- [-649.555] (-654.792) (-664.507) (-658.324) * (-647.553) (-654.119) [-650.391] (-651.587) -- 0:00:11 950000 -- [-649.488] (-663.178) (-656.611) (-649.440) * (-670.320) (-650.327) [-659.966] (-657.514) -- 0:00:11 Average standard deviation of split frequencies: 0.007509 951000 -- [-656.894] (-654.311) (-657.524) (-658.764) * (-648.985) [-648.018] (-654.284) (-659.506) -- 0:00:11 952000 -- (-654.506) (-653.311) (-668.238) [-650.344] * (-645.602) (-647.415) [-651.248] (-660.371) -- 0:00:10 953000 -- [-651.590] (-648.287) (-658.973) (-648.475) * (-651.222) (-654.517) (-655.015) [-658.786] -- 0:00:10 954000 -- (-647.165) (-660.731) (-659.920) [-656.468] * (-656.538) (-654.082) [-655.778] (-656.938) -- 0:00:10 955000 -- (-654.944) (-662.517) (-656.761) [-655.612] * (-652.399) (-650.142) (-651.270) [-660.917] -- 0:00:10 Average standard deviation of split frequencies: 0.006868 956000 -- (-651.661) [-655.990] (-652.713) (-663.305) * [-646.609] (-649.996) (-658.339) (-659.129) -- 0:00:09 957000 -- [-649.649] (-660.325) (-658.777) (-662.693) * (-664.469) (-656.807) [-653.711] (-655.739) -- 0:00:09 958000 -- (-651.824) (-660.238) (-657.409) [-663.427] * (-649.407) [-650.327] (-660.705) (-657.780) -- 0:00:09 959000 -- (-648.536) (-653.176) (-655.917) [-650.055] * [-648.793] (-654.163) (-659.455) (-648.850) -- 0:00:09 960000 -- (-650.766) [-651.590] (-647.929) (-662.703) * (-649.301) (-650.371) (-663.691) [-649.785] -- 0:00:09 Average standard deviation of split frequencies: 0.006730 961000 -- [-652.574] (-657.772) (-652.315) (-659.063) * [-649.821] (-659.640) (-662.967) (-662.958) -- 0:00:08 962000 -- [-653.218] (-655.390) (-648.959) (-664.392) * (-649.443) (-655.900) [-651.115] (-660.184) -- 0:00:08 963000 -- (-651.441) (-649.100) [-654.026] (-649.383) * [-648.629] (-657.115) (-656.080) (-657.563) -- 0:00:08 964000 -- [-652.798] (-648.727) (-666.713) (-667.785) * [-660.638] (-660.961) (-650.221) (-652.589) -- 0:00:08 965000 -- [-653.047] (-650.691) (-658.132) (-652.580) * (-653.282) (-646.890) (-670.716) [-652.055] -- 0:00:07 Average standard deviation of split frequencies: 0.006309 966000 -- [-653.008] (-654.866) (-651.712) (-653.771) * (-647.941) [-661.256] (-656.090) (-650.235) -- 0:00:07 967000 -- (-647.034) (-665.106) (-650.264) [-653.223] * (-656.824) (-654.452) [-652.612] (-648.263) -- 0:00:07 968000 -- (-656.553) (-654.843) [-652.232] (-661.866) * (-652.405) (-650.879) [-654.221] (-657.041) -- 0:00:07 969000 -- (-651.870) (-660.758) [-653.112] (-656.778) * (-653.309) [-656.209] (-653.546) (-654.020) -- 0:00:07 970000 -- (-653.748) (-652.192) [-647.922] (-652.110) * (-654.445) (-652.884) [-647.303] (-658.357) -- 0:00:06 Average standard deviation of split frequencies: 0.006036 971000 -- (-647.622) (-656.444) (-661.678) [-653.573] * [-646.539] (-650.293) (-646.273) (-657.550) -- 0:00:06 972000 -- (-653.560) (-648.722) [-653.155] (-658.596) * (-653.710) (-649.640) (-651.146) [-649.590] -- 0:00:06 973000 -- (-657.530) (-662.363) [-654.128] (-658.647) * [-651.663] (-650.168) (-646.447) (-659.015) -- 0:00:06 974000 -- (-652.778) [-653.012] (-657.489) (-672.423) * (-651.424) (-649.341) (-655.651) [-660.699] -- 0:00:05 975000 -- (-658.084) [-653.330] (-653.293) (-662.520) * [-651.371] (-663.337) (-654.867) (-652.323) -- 0:00:05 Average standard deviation of split frequencies: 0.006520 976000 -- (-654.373) [-650.160] (-657.996) (-670.955) * [-653.003] (-664.000) (-652.118) (-650.621) -- 0:00:05 977000 -- (-658.286) (-661.531) [-648.607] (-666.561) * (-653.959) (-659.135) [-651.603] (-653.121) -- 0:00:05 978000 -- (-652.113) (-651.033) (-653.718) [-657.272] * (-650.495) [-649.795] (-654.940) (-650.559) -- 0:00:04 979000 -- (-652.279) (-651.960) [-651.682] (-659.073) * (-661.149) (-652.498) (-660.499) [-650.901] -- 0:00:04 980000 -- (-663.219) (-654.025) [-651.233] (-661.359) * [-649.882] (-653.951) (-658.714) (-654.560) -- 0:00:04 Average standard deviation of split frequencies: 0.006558 981000 -- (-648.485) [-650.544] (-653.324) (-654.224) * (-652.460) [-654.398] (-655.983) (-656.624) -- 0:00:04 982000 -- (-654.762) (-650.660) [-650.284] (-658.412) * (-651.847) (-654.174) (-655.062) [-652.415] -- 0:00:04 983000 -- [-649.060] (-654.986) (-648.284) (-656.212) * [-653.617] (-650.787) (-658.185) (-649.999) -- 0:00:03 984000 -- (-653.936) (-655.825) [-649.932] (-667.103) * (-657.510) [-645.436] (-655.884) (-666.393) -- 0:00:03 985000 -- (-656.570) (-656.410) [-647.488] (-652.156) * (-652.933) [-652.460] (-655.097) (-656.885) -- 0:00:03 Average standard deviation of split frequencies: 0.006352 986000 -- [-654.562] (-654.569) (-651.821) (-660.419) * [-652.673] (-651.837) (-661.531) (-662.659) -- 0:00:03 987000 -- [-654.569] (-659.214) (-649.201) (-662.504) * (-653.510) (-653.882) (-659.050) [-654.440] -- 0:00:02 988000 -- [-651.403] (-653.466) (-652.612) (-656.641) * [-648.442] (-664.465) (-646.669) (-649.768) -- 0:00:02 989000 -- (-652.721) [-655.089] (-650.502) (-655.087) * [-649.002] (-656.458) (-655.435) (-648.774) -- 0:00:02 990000 -- (-663.850) (-655.058) (-654.592) [-654.952] * [-651.371] (-658.696) (-652.208) (-653.558) -- 0:00:02 Average standard deviation of split frequencies: 0.006424 991000 -- (-649.548) (-657.110) [-649.581] (-647.636) * (-654.693) (-654.750) (-651.225) [-648.676] -- 0:00:02 992000 -- (-656.350) (-660.717) [-644.667] (-654.578) * (-659.756) (-659.966) [-652.995] (-654.236) -- 0:00:01 993000 -- (-651.437) [-655.543] (-653.765) (-652.006) * [-651.939] (-658.998) (-653.436) (-650.399) -- 0:00:01 994000 -- (-650.709) (-649.519) (-653.122) [-653.714] * (-648.840) [-651.733] (-657.109) (-649.319) -- 0:00:01 995000 -- (-652.387) (-653.571) [-651.749] (-652.655) * (-648.973) [-652.048] (-649.814) (-655.128) -- 0:00:01 Average standard deviation of split frequencies: 0.006660 996000 -- (-650.072) (-655.603) (-649.293) [-659.589] * (-658.410) (-652.955) [-648.071] (-652.516) -- 0:00:00 997000 -- [-649.873] (-662.932) (-649.258) (-656.929) * (-654.847) (-644.942) [-657.584] (-661.872) -- 0:00:00 998000 -- (-655.421) (-656.038) (-651.194) [-646.286] * (-650.199) (-651.686) (-651.585) [-654.494] -- 0:00:00 999000 -- (-659.444) (-654.436) (-651.175) [-651.348] * (-657.291) [-654.052] (-661.673) (-647.583) -- 0:00:00 1000000 -- (-654.515) (-657.154) [-648.364] (-662.206) * (-646.847) (-654.168) [-649.116] (-650.721) -- 0:00:00 Average standard deviation of split frequencies: 0.006595 Analysis completed in 3 mins 47 seconds Analysis used 226.85 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -641.52 Likelihood of best state for "cold" chain of run 2 was -641.72 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 69.9 % ( 70 %) Dirichlet(Revmat{all}) 85.4 % ( 76 %) Slider(Revmat{all}) 36.7 % ( 29 %) Dirichlet(Pi{all}) 37.2 % ( 31 %) Slider(Pi{all}) 79.0 % ( 50 %) Multiplier(Alpha{1,2}) 74.1 % ( 53 %) Multiplier(Alpha{3}) 80.8 % ( 61 %) Slider(Pinvar{all}) 57.3 % ( 63 %) ExtSPR(Tau{all},V{all}) 59.9 % ( 55 %) ExtTBR(Tau{all},V{all}) 67.3 % ( 74 %) NNI(Tau{all},V{all}) 51.6 % ( 47 %) ParsSPR(Tau{all},V{all}) 27.3 % ( 21 %) Multiplier(V{all}) 67.9 % ( 75 %) Nodeslider(V{all}) 27.3 % ( 19 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 68.1 % ( 68 %) Dirichlet(Revmat{all}) 84.8 % ( 81 %) Slider(Revmat{all}) 35.9 % ( 27 %) Dirichlet(Pi{all}) 37.5 % ( 28 %) Slider(Pi{all}) 79.7 % ( 61 %) Multiplier(Alpha{1,2}) 74.4 % ( 54 %) Multiplier(Alpha{3}) 80.0 % ( 65 %) Slider(Pinvar{all}) 57.4 % ( 58 %) ExtSPR(Tau{all},V{all}) 59.8 % ( 69 %) ExtTBR(Tau{all},V{all}) 67.4 % ( 71 %) NNI(Tau{all},V{all}) 51.5 % ( 46 %) ParsSPR(Tau{all},V{all}) 27.3 % ( 24 %) Multiplier(V{all}) 67.7 % ( 64 %) Nodeslider(V{all}) 27.6 % ( 31 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.71 0.48 0.30 2 | 166399 0.73 0.50 3 | 166476 166649 0.74 4 | 166764 166997 166715 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.71 0.48 0.30 2 | 166197 0.73 0.50 3 | 167170 166605 0.74 4 | 166796 166294 166938 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -650.03 | 1 2 | | 1 | | 2 2 22 2 1 | | * * 2 | | 2 21 * 2 1 *1 1 2 2 | | 1 1 1 1 1 12 2 1 | | 1 1 1 1 2 * 2 2 2 1 1 11 *| |2* 2 2 22 2 1 2 2 2 2 2 2 22 | | 2 1 1 2 2 2 21 11 21 1 2 1 | | 1 * 1 1 1 1 1 2 1 | | 2 1 2 1 1 2 2 2 | | 12 2 * 1 111 1 | |1 2 2 2 | | 2 1 | | 1 1 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -653.83 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -647.96 -659.51 2 -647.57 -660.84 -------------------------------------- TOTAL -647.75 -660.38 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.093485 0.000608 0.051158 0.145246 0.089709 1233.22 1367.11 1.000 r(A<->C){all} 0.111862 0.004368 0.009992 0.243902 0.098514 413.26 503.82 1.001 r(A<->G){all} 0.171980 0.006443 0.037016 0.329354 0.161453 225.19 301.97 1.000 r(A<->T){all} 0.077741 0.003039 0.000138 0.182334 0.065728 331.14 359.21 1.001 r(C<->G){all} 0.037897 0.001388 0.000020 0.113160 0.025969 697.58 699.51 1.000 r(C<->T){all} 0.508286 0.010090 0.325719 0.712712 0.508815 435.29 452.39 1.000 r(G<->T){all} 0.092233 0.002688 0.007593 0.190577 0.083772 411.27 426.56 1.001 pi(A){all} 0.242394 0.000480 0.200817 0.286125 0.241944 1203.67 1209.77 1.000 pi(C){all} 0.249892 0.000504 0.207480 0.294509 0.249084 1209.08 1313.81 1.000 pi(G){all} 0.193492 0.000418 0.154276 0.233573 0.192852 1261.49 1296.09 1.000 pi(T){all} 0.314222 0.000559 0.268362 0.361642 0.314476 1042.28 1117.31 1.000 alpha{1,2} 0.581252 0.488578 0.000266 2.017942 0.334177 980.51 1125.10 1.000 alpha{3} 1.213948 1.083572 0.000506 3.216179 0.937521 817.45 964.24 1.000 pinvar{all} 0.568679 0.038574 0.160037 0.885355 0.610529 534.04 648.46 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C2 3 -- C3 4 -- C4 5 -- C5 6 -- C6 7 -- C7 8 -- C8 9 -- C9 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition --------------- 1 -- .******** 2 -- .*....... 3 -- ..*...... 4 -- ...*..... 5 -- ....*.... 6 -- .....*... 7 -- ......*.. 8 -- .......*. 9 -- ........* 10 -- ...*..*.. 11 -- .******.* 12 -- .*.****.* 13 -- .*.****** 14 -- ..******* 15 -- ..*....*. 16 -- .....*..* 17 -- ...**.*.. 18 -- ....*...* 19 -- ....**... 20 -- ...*..*.* 21 -- ...****.* 22 -- ...*.**.. 23 -- ...****** --------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 10 2887 0.961692 0.002355 0.960027 0.963358 2 11 1063 0.354097 0.004240 0.351099 0.357095 2 12 989 0.329447 0.008951 0.323118 0.335776 2 13 687 0.228847 0.006124 0.224517 0.233178 2 14 554 0.184544 0.020728 0.169887 0.199201 2 15 437 0.145570 0.006124 0.141239 0.149900 2 16 390 0.129913 0.002827 0.127915 0.131912 2 17 385 0.128248 0.008009 0.122585 0.133911 2 18 364 0.121252 0.001884 0.119920 0.122585 2 19 362 0.120586 0.007537 0.115256 0.125916 2 20 351 0.116922 0.012719 0.107928 0.125916 2 21 350 0.116589 0.000000 0.116589 0.116589 2 22 344 0.114590 0.001884 0.113258 0.115923 2 23 333 0.110926 0.008951 0.104597 0.117255 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.015382 0.000061 0.002787 0.030662 0.013927 1.001 2 length{all}[2] 0.011153 0.000032 0.002129 0.022261 0.010206 1.000 2 length{all}[3] 0.020693 0.000074 0.006649 0.038455 0.019217 1.000 2 length{all}[4] 0.002063 0.000005 0.000000 0.006445 0.001405 1.000 2 length{all}[5] 0.002049 0.000004 0.000000 0.006138 0.001400 1.000 2 length{all}[6] 0.002125 0.000005 0.000000 0.006633 0.001392 1.001 2 length{all}[7] 0.002102 0.000005 0.000001 0.006313 0.001443 1.000 2 length{all}[8] 0.017213 0.000067 0.003935 0.032976 0.015890 1.000 2 length{all}[9] 0.002049 0.000005 0.000002 0.006411 0.001369 1.000 2 length{all}[10] 0.004190 0.000010 0.000109 0.010464 0.003394 1.000 2 length{all}[11] 0.005262 0.000019 0.000018 0.013949 0.004282 1.001 2 length{all}[12] 0.004543 0.000012 0.000000 0.010655 0.003778 1.000 2 length{all}[13] 0.004692 0.000017 0.000008 0.013163 0.003399 0.999 2 length{all}[14] 0.005415 0.000020 0.000041 0.014131 0.004219 1.000 2 length{all}[15] 0.004070 0.000013 0.000002 0.010843 0.003187 1.000 2 length{all}[16] 0.001983 0.000004 0.000007 0.006098 0.001459 0.999 2 length{all}[17] 0.002054 0.000005 0.000003 0.006272 0.001386 1.000 2 length{all}[18] 0.002026 0.000005 0.000002 0.006261 0.001313 0.998 2 length{all}[19] 0.002092 0.000005 0.000006 0.006651 0.001370 0.997 2 length{all}[20] 0.002227 0.000006 0.000001 0.006332 0.001546 0.997 2 length{all}[21] 0.003176 0.000009 0.000013 0.009789 0.002199 0.997 2 length{all}[22] 0.002053 0.000004 0.000006 0.006015 0.001483 0.997 2 length{all}[23] 0.004601 0.000016 0.000023 0.011782 0.003375 1.015 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.006595 Maximum standard deviation of split frequencies = 0.020728 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.015 Clade credibility values: /------------------------------------------------------------------------ C1 (1) | |------------------------------------------------------------------------ C2 (2) | |------------------------------------------------------------------------ C3 (3) | |------------------------------------------------------------------------ C5 (5) | +------------------------------------------------------------------------ C6 (6) | |------------------------------------------------------------------------ C8 (8) | |------------------------------------------------------------------------ C9 (9) | | /------------------------------------ C4 (4) \-----------------96----------------+ \------------------------------------ C7 (7) Phylogram (based on average branch lengths): /---------------------------------------------------- C1 (1) | |-------------------------------------- C2 (2) | |------------------------------------------------------------------------ C3 (3) | |----- C5 (5) | +----- C6 (6) | |------------------------------------------------------------ C8 (8) | |----- C9 (9) | | /----- C4 (4) \------------+ \----- C7 (7) |-----------------| 0.005 expected changes per site Calculating tree probabilities... Credible sets of trees (1892 trees sampled): 50 % credible set contains 481 trees 90 % credible set contains 1592 trees 95 % credible set contains 1742 trees 99 % credible set contains 1862 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,C3,C5,C6,C8,C9,(C4,C7))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **9** sequences, **121** codons, and **1** partitions from `/data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -648.03, AIC-c = 1332.28 (18 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.087 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 2.815 * non-synonymous rate = 0.429 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results ---- ## FUBAR inferred no sites under subject to positive selection at posterior probability >= 0.9
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=9, Len=121 BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ------------------MMRVQRPPTLLLLLLVANAFSKPIGTPIPEHC BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ------------------MMRVQRPPTLLLILLVANAFSKPIGTSIPEHC BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLFLILLVANAFSKPIGTPIPEHC HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 -------------------MRVQRPPTLLLILLVANAFSKPIGTPIPEHC TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC *********:*:*************.:**** BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 STLAGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT ***.********************************************** BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQQLSDIGYSFDYGK HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 SYDNSDPQSLSDIGYSFDYGK TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 SYDTSDPQLLSDIGYSFDYGK ***.**** ************
>BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ------------------------------------------------------ATGATGCGTGTGCAAAGACCACCCACACTCCTGCTTCTCTTACTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTCCTATACCTGAACACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGATACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ------------------------------------------------------ATGATGCGTGTGCAAAGACCACCCACACTTCTGCTCATATTATTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTTCTATACCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGATGAGTATGAGGTCGAGAAGATCCATGTTCATTGAGCATTTTAACGAACTTATGATGCGTGTGCAAAGACCACCCACACTCTTTCTCATCTTACTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACCCCTATACCTGAGCACTGTTCTACCCTTGCTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACAGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGCAGTTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGATGAGTATGAGGTCGAGAAGATCCATGTTCATTGAGCATTTTAACGAACTTATGATGCGTGTGCAAAGACCACCCACACTCTTGCTCATTTTATTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTCCTATGCCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGATGAGTATGAGGTCGAGAAGATCCATGTTCATTGAGCATTTTAACGAACTTATGATGCGTGTGCAAAGACCACCCACACTCTTGCTCATTTTATTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTCCTATACCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGATGAGTATGAGGTCGAGAAGATCCATGTTCATTGAGCATTTTAACGAACTTATGATGCGTGTGCAAAGACCACCCACACTCTTGCTCATTTTATTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTCCTATACCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGATGAGTATGAGGTCGAGAAGATCCATGTTCATTGAGCATTTTAACGAACTTATGATGCGTGTGCAAAGACCACCCACACTCTTGCTCATTTTATTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTCCTATGCCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ---------------------------------------------------------ATGCGTGTGCAAAGACCACCCACACTCTTGCTCATTTTACTAGTTGCTAATGCTTTTTCAAAGCCCATTGGCACTCCTATACCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTTATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGATACCTCTTACGACAATTCTGACCCTCAGTCTCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG >TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGATGAGTATGAGGTCGAGAAGATCCATGTTCATTGAGCATTTTAACGAACTTATGATGCGTGTGCAAAGACCACCCACACTCTTGCTCATTTTATTAGTTGCTAATGCTTTCTCAAAGCCCATTGGCACTCCTATACCTGAGCACTGTTCTACCCTTGTTGGTAGAGAGTTTCAATCTTGTATTCGACAAGCACAGTTTGATACGGCTGGCATGTACACTAATCGCGTCATCGTGCTCGATCGAGCACGTACTCATCGCTATCCTATTGATCGTGACACTACTACTGCTCACGACACCTCTTACGACACTTCTGACCCTCAGTTGCTGTCTGATATTGGTTACTCCTTTGATTATGGGAAG
>BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ------------------MMRVQRPPTLLLLLLVANAFSKPIGTPIPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK >BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ------------------MMRVQRPPTLLLILLVANAFSKPIGTSIPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK >BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLFLILLVANAFSKPIGTPIPEHC STLAGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQQLSDIGYSFDYGK >HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK >TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK >TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK >LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPMPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK >YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 -------------------MRVQRPPTLLLILLVANAFSKPIGTPIPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDNSDPQSLSDIGYSFDYGK >TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MMSMRSRRSMFIEHFNELMMRVQRPPTLLLILLVANAFSKPIGTPIPEHC STLVGREFQSCIRQAQFDTAGMYTNRVIVLDRARTHRYPIDRDTTTAHDT SYDTSDPQLLSDIGYSFDYGK
Reading sequence file /data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/fasta/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1 Found 9 sequences of length 363 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 1.9% Found 5 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 1 Calculating analytical mean and variance Doing permutation test for PHI Doing permutation test for NSS Doing Permutation test for MAXCHI Writing alignment of polymorphic unambig sites to: Phi.poly.sites Window size is 14 polymorphic sites **p-Value(s)** ---------- NSS: 1.72e-01 (1000 permutations) Max Chi^2: 6.90e-01 (1000 permutations) PHI (Permutation): 1.90e-01 (1000 permutations) PHI (Normal): 5.44e-02
#NEXUS [ID: 4389161250] begin taxa; dimensions ntax=9; taxlabels BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ; end; begin trees; translate 1 BY140562_orf3_AWH65922_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5, 2 BY140535_orf3_AWH65911_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5, 3 BtPa_GD2013_NA_AIA62344_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 4 HKU5_1_LMH03f_NS3a_YP_001039963_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 5 TT06f_NS3a_ABN10894_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 6 TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 7 LMH03f_NS3a_ABN10876_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 8 YD13403_orf3_AWH65933_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5, 9 TT07f_NS3a_ABN10903_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:1.392705e-02,2:1.020632e-02,3:1.921686e-02,5:1.400109e-03,6:1.391693e-03,8:1.589043e-02,9:1.368947e-03,(4:1.405241e-03,7:1.443454e-03)0.962:3.393578e-03); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:1.392705e-02,2:1.020632e-02,3:1.921686e-02,5:1.400109e-03,6:1.391693e-03,8:1.589043e-02,9:1.368947e-03,(4:1.405241e-03,7:1.443454e-03):3.393578e-03); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -647.82 -660.96 2 -647.93 -665.33 -------------------------------------- TOTAL -647.87 -664.65 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.094619 0.000665 0.050248 0.148044 0.091328 1421.47 1441.77 1.000 r(A<->C){all} 0.105840 0.003778 0.008375 0.228855 0.094510 316.81 425.09 1.000 r(A<->G){all} 0.172186 0.006908 0.032141 0.335241 0.159813 318.51 361.90 1.000 r(A<->T){all} 0.080640 0.002776 0.001671 0.178999 0.071481 327.85 469.68 1.001 r(C<->G){all} 0.039367 0.001549 0.000008 0.119285 0.027322 349.85 497.00 1.001 r(C<->T){all} 0.505276 0.010267 0.310899 0.698400 0.505749 446.42 470.40 1.000 r(G<->T){all} 0.096691 0.003135 0.005646 0.203415 0.087039 462.70 479.18 1.002 pi(A){all} 0.241701 0.000468 0.199043 0.283782 0.241689 1119.63 1226.01 1.000 pi(C){all} 0.250569 0.000503 0.208812 0.296062 0.249816 1173.45 1223.01 1.001 pi(G){all} 0.192929 0.000398 0.155918 0.233177 0.192453 1288.05 1294.35 1.000 pi(T){all} 0.314801 0.000550 0.271634 0.361426 0.313900 1199.69 1217.12 1.000 alpha{1,2} 0.600158 0.564324 0.000015 2.072402 0.337430 1044.67 1169.53 1.000 alpha{3} 1.197001 1.047704 0.000705 3.243417 0.934710 925.73 1004.67 1.000 pinvar{all} 0.572390 0.037415 0.169863 0.876821 0.605757 745.32 758.27 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,C3,C5,C6,C8,C9,(C4,C7))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **9** sequences, **121** codons, and **1** partitions from `/data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3a_ABN10885_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -648.03, AIC-c = 1332.28 (18 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.087 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 2.815 * non-synonymous rate = 0.429 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results ---- ## FUBAR inferred no sites under subject to positive selection at posterior probability >= 0.9
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500