--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -661.20 -675.31 2 -661.42 -674.82 -------------------------------------- TOTAL -661.31 -675.09 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.244417 0.001925 0.166413 0.333500 0.241348 1347.97 1379.67 1.000 r(A<->C){all} 0.130756 0.002910 0.034473 0.232751 0.124507 547.35 611.46 1.001 r(A<->G){all} 0.151664 0.003032 0.051761 0.257343 0.147630 536.08 614.59 1.001 r(A<->T){all} 0.107938 0.001444 0.037894 0.183019 0.103776 501.22 658.85 1.000 r(C<->G){all} 0.194736 0.004460 0.070312 0.322834 0.190441 595.91 620.68 1.000 r(C<->T){all} 0.305273 0.004349 0.185833 0.437422 0.303200 678.59 692.81 1.000 r(G<->T){all} 0.109632 0.001903 0.035757 0.201501 0.104854 426.53 570.62 1.000 pi(A){all} 0.256399 0.000603 0.211168 0.305750 0.255863 1142.95 1208.62 1.000 pi(C){all} 0.196613 0.000483 0.153181 0.240209 0.196081 862.43 1000.99 1.000 pi(G){all} 0.176108 0.000482 0.133979 0.217289 0.175082 1074.12 1168.91 1.000 pi(T){all} 0.370880 0.000760 0.321745 0.426653 0.369967 1141.06 1180.20 1.000 alpha{1,2} 0.660894 0.486095 0.001693 2.010291 0.442134 1107.58 1143.40 1.001 alpha{3} 1.954139 1.360016 0.304380 4.298999 1.691177 1110.77 1305.88 1.000 pinvar{all} 0.187144 0.017825 0.000136 0.440395 0.162519 955.53 1021.50 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Wed Oct 26 22:52:41 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=10, Len=91 C1 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C2 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C3 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C4 MVSFNVTAILLVLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C5 ---MNVTAILLQNVANAFSNPLYVPEHCVGMPGTLFQACIRQTMVDTTGM C6 ---MNVTAILLQNVANAFSNPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C7 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C8 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C9 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C10 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSATLFQACIRQTMVDTTGM :*.***:* :*****:******** **..***************** C1 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C2 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C3 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C4 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV C5 YTNSAMSYDGTTIPFDIHGIVHEDHYTDTKPTPLSDVGFSV C6 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV C7 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C8 YTNSAMSHDGITIPFDRDGIVHEEHYTETNPTPLSDVGFSV C9 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C10 YTNSDMSHDGITIPFDRDGIVHEEHYTETNPTPLFDAGFSV **** **:** ***** .*****:***:*:**** *.**** -- Starting log on Wed Oct 26 22:53:24 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.08 sec, SCORE=996, Nseq=10, Len=91 C1 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C2 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C3 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C4 MVSFNVTAILLVLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C5 ---MNVTAILLQNVANAFSNPLYVPEHCVGMPGTLFQACIRQTMVDTTGM C6 ---MNVTAILLQNVANAFSNPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C7 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C8 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C9 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C10 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSATLFQACIRQTMVDTTGM :*.***:* :*****:******** **..***************** C1 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C2 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C3 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C4 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV C5 YTNSAMSYDGTTIPFDIHGIVHEDHYTDTKPTPLSDVGFSV C6 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV C7 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C8 YTNSAMSHDGITIPFDRDGIVHEEHYTETNPTPLSDVGFSV C9 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C10 YTNSDMSHDGITIPFDRDGIVHEEHYTETNPTPLFDAGFSV **** **:** ***** .*****:***:*:**** *.**** -- Starting log on Wed Oct 26 22:52:41 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=10, Len=91 C1 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C2 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C3 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C4 MVSFNVTAILLVLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C5 ---MNVTAILLQNVANAFSNPLYVPEHCVGMPGTLFQACIRQTMVDTTGM C6 ---MNVTAILLQNVANAFSNPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C7 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C8 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM C9 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM C10 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSATLFQACIRQTMVDTTGM :*.***:* :*****:******** **..***************** C1 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C2 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C3 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C4 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV C5 YTNSAMSYDGTTIPFDIHGIVHEDHYTDTKPTPLSDVGFSV C6 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV C7 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C8 YTNSAMSHDGITIPFDRDGIVHEEHYTETNPTPLSDVGFSV C9 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV C10 YTNSDMSHDGITIPFDRDGIVHEEHYTETNPTPLFDAGFSV **** **:** ***** .*****:***:*:**** *.**** -- Starting log on Wed Oct 26 23:06:01 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/gapped_alignment/fubar,B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 10 taxa and 273 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C10 Taxon 3 -> C2 Taxon 4 -> C3 Taxon 5 -> C4 Taxon 6 -> C5 Taxon 7 -> C6 Taxon 8 -> C7 Taxon 9 -> C8 Taxon 10 -> C9 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1666825564 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 520974034 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 1938929574 Seed = 31175006 Swapseed = 1666825564 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 16 unique site patterns Division 2 has 15 unique site patterns Division 3 has 29 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -956.577873 -- 35.653401 Chain 2 -- -1023.877308 -- 35.653401 Chain 3 -- -1027.559481 -- 35.653401 Chain 4 -- -1059.815658 -- 35.653401 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -997.968083 -- 35.653401 Chain 2 -- -1014.985740 -- 35.653401 Chain 3 -- -965.025031 -- 35.653401 Chain 4 -- -1048.726579 -- 35.653401 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-956.578] (-1023.877) (-1027.559) (-1059.816) * [-997.968] (-1014.986) (-965.025) (-1048.727) 1000 -- (-676.165) [-671.111] (-672.250) (-674.866) * (-674.653) (-662.489) [-669.111] (-672.131) -- 0:00:00 2000 -- (-670.888) (-663.255) (-666.998) [-668.190] * [-667.813] (-668.382) (-676.018) (-664.960) -- 0:00:00 3000 -- (-675.865) (-671.533) (-670.451) [-665.029] * (-668.390) [-668.825] (-665.019) (-663.424) -- 0:00:00 4000 -- (-678.286) [-667.247] (-665.873) (-670.259) * (-672.221) (-670.134) (-675.791) [-669.431] -- 0:04:09 5000 -- (-677.424) (-672.543) [-662.862] (-667.347) * (-666.768) [-667.825] (-669.446) (-663.848) -- 0:03:19 Average standard deviation of split frequencies: 0.040739 6000 -- (-666.499) (-669.337) (-672.400) [-662.060] * (-669.967) [-660.614] (-666.669) (-669.464) -- 0:02:45 7000 -- (-667.437) [-667.088] (-667.645) (-665.595) * (-667.854) (-670.651) [-665.030] (-682.763) -- 0:02:21 8000 -- (-661.424) (-679.101) [-669.142] (-668.603) * (-674.523) (-670.934) [-661.958] (-665.879) -- 0:04:08 9000 -- [-671.736] (-664.587) (-663.072) (-669.213) * [-662.595] (-667.570) (-670.864) (-663.016) -- 0:03:40 10000 -- [-666.329] (-666.434) (-665.375) (-681.505) * (-671.968) (-661.750) (-665.676) [-662.642] -- 0:03:18 Average standard deviation of split frequencies: 0.082864 11000 -- [-667.790] (-682.508) (-666.162) (-674.679) * (-667.700) (-681.304) (-671.168) [-663.828] -- 0:04:29 12000 -- (-671.430) [-662.479] (-666.448) (-663.411) * (-669.939) (-660.480) (-669.735) [-665.601] -- 0:04:07 13000 -- (-674.169) (-667.597) [-660.864] (-669.473) * (-669.361) (-671.046) (-673.349) [-675.364] -- 0:03:47 14000 -- (-662.990) [-669.003] (-659.030) (-670.506) * (-671.578) (-664.787) (-666.139) [-663.792] -- 0:03:31 15000 -- (-675.197) (-664.035) [-659.809] (-662.794) * (-668.103) (-673.487) [-664.716] (-666.776) -- 0:04:22 Average standard deviation of split frequencies: 0.065725 16000 -- (-668.363) (-665.105) (-666.359) [-662.568] * (-680.974) [-669.759] (-665.119) (-666.668) -- 0:04:06 17000 -- (-669.679) (-670.604) [-667.068] (-666.164) * (-669.278) (-664.216) (-667.773) [-666.343] -- 0:03:51 18000 -- (-661.830) (-665.344) [-669.393] (-668.282) * (-669.398) (-666.430) [-668.399] (-666.262) -- 0:03:38 19000 -- (-674.471) (-668.184) (-664.693) [-672.599] * (-663.986) [-661.851] (-673.021) (-675.485) -- 0:04:18 20000 -- (-667.785) (-663.709) [-666.002] (-683.884) * (-668.564) (-669.571) [-667.169] (-668.343) -- 0:04:05 Average standard deviation of split frequencies: 0.057025 21000 -- [-669.521] (-658.842) (-673.346) (-670.968) * [-662.238] (-671.756) (-664.008) (-668.389) -- 0:03:53 22000 -- (-668.991) (-672.069) [-662.071] (-668.258) * (-668.282) (-661.388) (-671.687) [-669.755] -- 0:04:26 23000 -- (-661.682) [-665.381] (-664.287) (-664.603) * [-661.484] (-673.579) (-669.652) (-666.604) -- 0:04:14 24000 -- (-675.950) [-663.826] (-672.826) (-662.557) * (-667.250) [-658.840] (-670.369) (-670.803) -- 0:04:04 25000 -- (-666.591) (-668.434) (-658.988) [-667.183] * (-669.667) (-668.795) (-662.251) [-667.299] -- 0:03:54 Average standard deviation of split frequencies: 0.042064 26000 -- (-663.943) [-663.022] (-665.048) (-668.837) * (-673.398) (-671.251) [-661.865] (-666.371) -- 0:04:22 27000 -- (-664.717) (-671.996) (-661.402) [-665.425] * (-664.482) (-685.869) (-671.427) [-671.725] -- 0:04:12 28000 -- (-674.741) (-662.950) [-665.343] (-673.606) * (-665.941) [-669.580] (-666.138) (-663.467) -- 0:04:03 29000 -- (-664.649) (-667.361) [-671.599] (-668.417) * (-663.356) (-674.542) [-667.949] (-665.292) -- 0:03:54 30000 -- [-664.927] (-664.431) (-668.657) (-666.335) * (-671.137) (-678.457) (-667.365) [-669.514] -- 0:04:18 Average standard deviation of split frequencies: 0.035048 31000 -- (-672.568) [-665.046] (-667.529) (-663.433) * (-666.598) (-664.459) [-663.143] (-668.144) -- 0:04:10 32000 -- (-667.860) (-666.833) [-665.440] (-661.896) * (-668.283) (-679.983) (-665.294) [-658.755] -- 0:04:02 33000 -- (-669.201) (-670.206) [-663.798] (-674.398) * [-674.221] (-666.802) (-672.031) (-664.279) -- 0:03:54 34000 -- [-665.986] (-672.395) (-669.249) (-663.445) * (-665.801) (-670.675) [-668.919] (-672.631) -- 0:04:15 35000 -- (-665.976) (-674.642) (-664.356) [-661.719] * (-671.799) [-666.347] (-671.601) (-674.642) -- 0:04:08 Average standard deviation of split frequencies: 0.034373 36000 -- (-675.565) (-677.217) (-664.677) [-663.051] * (-671.692) [-660.888] (-665.790) (-668.914) -- 0:04:01 37000 -- (-670.633) (-678.389) [-664.520] (-674.367) * (-669.524) (-662.562) [-668.150] (-671.710) -- 0:03:54 38000 -- [-666.364] (-675.060) (-671.034) (-667.836) * (-663.769) [-665.380] (-663.701) (-677.393) -- 0:04:13 39000 -- [-664.917] (-669.885) (-668.140) (-674.689) * [-671.464] (-670.807) (-664.063) (-672.798) -- 0:04:06 40000 -- (-677.936) (-669.644) [-667.383] (-664.119) * [-670.753] (-666.870) (-665.471) (-665.158) -- 0:04:00 Average standard deviation of split frequencies: 0.032844 41000 -- [-670.093] (-665.852) (-667.713) (-668.614) * (-665.561) (-678.530) (-676.277) [-666.217] -- 0:04:17 42000 -- (-674.690) [-661.778] (-668.126) (-666.681) * (-673.488) (-669.310) (-672.431) [-662.240] -- 0:04:10 43000 -- (-663.498) (-669.766) (-667.796) [-658.860] * [-670.282] (-669.363) (-671.802) (-659.794) -- 0:04:04 44000 -- (-668.548) (-674.147) (-668.006) [-662.402] * (-668.848) [-669.446] (-665.024) (-664.425) -- 0:03:59 45000 -- [-669.270] (-663.907) (-669.240) (-674.072) * (-671.581) [-665.057] (-666.838) (-666.360) -- 0:04:14 Average standard deviation of split frequencies: 0.029036 46000 -- (-663.217) (-671.568) [-676.619] (-668.556) * (-665.682) (-672.858) (-677.574) [-675.299] -- 0:04:08 47000 -- (-664.052) [-666.204] (-669.333) (-668.903) * (-667.387) [-665.143] (-671.219) (-670.514) -- 0:04:03 48000 -- (-669.452) (-670.578) (-670.789) [-666.861] * (-673.658) [-663.870] (-666.610) (-673.125) -- 0:03:58 49000 -- (-672.922) (-669.857) [-669.498] (-678.325) * (-667.655) [-669.972] (-669.279) (-679.642) -- 0:04:12 50000 -- (-662.365) [-665.328] (-667.907) (-670.517) * (-672.362) (-677.626) [-663.160] (-681.460) -- 0:04:06 Average standard deviation of split frequencies: 0.029935 51000 -- (-669.886) (-667.122) (-671.812) [-665.826] * (-681.500) [-664.628] (-671.238) (-672.345) -- 0:04:01 52000 -- (-666.557) (-663.675) [-673.755] (-674.476) * (-672.771) [-664.729] (-664.544) (-675.458) -- 0:03:57 53000 -- [-671.624] (-672.649) (-672.043) (-670.139) * (-681.995) [-665.841] (-662.037) (-669.977) -- 0:04:10 54000 -- (-671.147) (-665.656) (-669.496) [-672.958] * (-670.042) (-679.331) [-670.039] (-668.795) -- 0:04:05 55000 -- [-662.874] (-674.052) (-669.966) (-665.672) * (-666.888) (-669.511) [-665.102] (-664.153) -- 0:04:00 Average standard deviation of split frequencies: 0.030744 56000 -- (-668.109) (-671.716) [-661.048] (-669.350) * (-663.336) (-665.758) [-670.773] (-675.191) -- 0:03:56 57000 -- [-664.742] (-673.424) (-665.535) (-674.716) * (-665.719) (-665.553) (-677.490) [-666.095] -- 0:04:08 58000 -- (-671.704) (-670.560) [-675.420] (-670.207) * (-667.204) [-669.729] (-674.518) (-663.298) -- 0:04:03 59000 -- (-662.736) (-671.153) (-673.358) [-665.005] * [-660.110] (-667.080) (-668.242) (-672.846) -- 0:03:59 60000 -- (-672.082) [-665.735] (-667.631) (-671.539) * (-664.434) [-665.617] (-664.802) (-663.148) -- 0:04:10 Average standard deviation of split frequencies: 0.027027 61000 -- (-669.372) [-664.398] (-670.079) (-672.204) * [-664.407] (-671.138) (-663.206) (-663.483) -- 0:04:06 62000 -- (-667.147) (-669.082) (-673.725) [-670.177] * (-665.404) (-671.384) [-662.137] (-665.014) -- 0:04:02 63000 -- [-666.914] (-665.366) (-663.107) (-673.807) * (-665.110) (-659.930) (-674.570) [-665.679] -- 0:03:57 64000 -- (-670.389) [-664.547] (-664.053) (-672.014) * (-674.660) (-663.436) [-660.229] (-679.915) -- 0:04:08 65000 -- (-663.281) (-671.075) [-667.993] (-671.456) * [-669.747] (-668.113) (-663.843) (-672.839) -- 0:04:04 Average standard deviation of split frequencies: 0.024284 66000 -- (-666.539) (-666.427) (-663.338) [-663.682] * [-672.583] (-661.454) (-664.843) (-661.397) -- 0:04:00 67000 -- (-669.552) [-664.494] (-662.542) (-666.348) * (-669.080) [-664.469] (-659.777) (-661.980) -- 0:03:56 68000 -- (-665.628) (-660.915) [-664.989] (-665.860) * (-667.941) [-668.971] (-661.311) (-665.344) -- 0:04:06 69000 -- [-662.361] (-664.737) (-672.234) (-677.961) * (-668.936) (-658.446) [-669.677] (-663.516) -- 0:04:02 70000 -- (-661.884) [-667.683] (-663.631) (-664.733) * (-668.135) (-662.545) [-660.569] (-674.994) -- 0:03:59 Average standard deviation of split frequencies: 0.027484 71000 -- (-669.428) (-665.615) [-673.525] (-664.746) * [-661.437] (-670.911) (-666.908) (-664.501) -- 0:03:55 72000 -- (-669.388) (-670.302) (-669.414) [-662.690] * (-674.385) (-677.459) (-663.994) [-669.705] -- 0:04:04 73000 -- [-666.919] (-675.850) (-674.521) (-670.014) * (-675.675) (-678.295) [-663.390] (-674.928) -- 0:04:01 74000 -- (-669.077) (-665.027) [-658.774] (-662.346) * (-675.722) (-665.264) (-670.283) [-671.381] -- 0:03:57 75000 -- (-662.639) (-668.371) [-666.725] (-664.967) * [-667.179] (-664.675) (-669.046) (-659.809) -- 0:04:06 Average standard deviation of split frequencies: 0.024035 76000 -- (-668.326) (-686.135) [-669.117] (-667.786) * (-667.150) (-662.285) (-674.599) [-660.979] -- 0:04:03 77000 -- (-669.804) [-670.275] (-670.431) (-671.110) * [-667.368] (-663.061) (-668.777) (-668.014) -- 0:03:59 78000 -- (-669.410) (-669.937) [-674.158] (-664.996) * (-667.629) [-659.438] (-672.749) (-668.177) -- 0:03:56 79000 -- (-671.174) (-664.758) [-670.052] (-662.743) * (-667.894) (-667.932) [-670.629] (-681.123) -- 0:04:04 80000 -- (-672.131) [-666.983] (-665.014) (-679.627) * (-669.207) (-669.097) (-671.260) [-663.736] -- 0:04:01 Average standard deviation of split frequencies: 0.024593 81000 -- (-675.224) [-668.702] (-669.866) (-668.428) * [-664.988] (-663.773) (-661.989) (-674.590) -- 0:03:58 82000 -- (-676.834) (-672.597) (-673.570) [-659.271] * (-671.287) (-668.139) [-669.748] (-665.316) -- 0:03:55 83000 -- (-672.448) (-672.859) (-672.320) [-670.686] * (-661.850) [-659.675] (-670.859) (-674.934) -- 0:04:03 84000 -- (-674.703) (-672.074) (-662.186) [-667.393] * (-663.920) [-661.511] (-667.095) (-670.749) -- 0:03:59 85000 -- (-666.907) (-679.924) [-667.066] (-666.796) * (-672.861) (-665.292) [-673.352] (-676.617) -- 0:03:56 Average standard deviation of split frequencies: 0.022584 86000 -- [-666.884] (-665.240) (-668.363) (-668.668) * (-670.531) [-665.063] (-665.519) (-678.081) -- 0:03:53 87000 -- [-668.199] (-669.695) (-668.788) (-673.759) * (-679.764) (-672.222) [-665.699] (-672.352) -- 0:04:01 88000 -- (-670.772) [-669.678] (-664.195) (-668.321) * (-669.894) (-667.630) (-664.971) [-669.134] -- 0:03:58 89000 -- (-669.639) (-669.301) (-665.062) [-661.781] * (-666.281) (-664.975) [-669.214] (-670.091) -- 0:03:55 90000 -- (-666.270) [-666.860] (-669.327) (-666.494) * (-681.132) (-666.100) [-662.390] (-665.589) -- 0:04:02 Average standard deviation of split frequencies: 0.023284 91000 -- (-664.973) (-672.904) [-664.277] (-667.124) * (-674.396) (-673.793) [-666.708] (-667.523) -- 0:03:59 92000 -- (-664.656) (-668.790) [-663.338] (-666.947) * (-668.808) (-670.267) (-680.622) [-665.217] -- 0:03:56 93000 -- (-659.254) (-665.361) (-666.908) [-665.477] * (-673.043) (-670.595) (-667.202) [-668.208] -- 0:03:54 94000 -- (-666.431) [-668.988] (-663.170) (-673.000) * [-664.184] (-668.357) (-670.013) (-665.579) -- 0:04:00 95000 -- (-676.028) (-664.907) (-664.614) [-668.409] * (-670.406) (-660.022) [-667.685] (-673.117) -- 0:03:58 Average standard deviation of split frequencies: 0.025985 96000 -- (-676.155) (-667.951) [-666.563] (-676.019) * (-664.062) [-668.143] (-663.952) (-673.969) -- 0:03:55 97000 -- [-666.022] (-677.742) (-675.098) (-668.067) * (-660.732) [-663.024] (-676.859) (-666.531) -- 0:03:52 98000 -- (-668.469) (-667.299) (-682.368) [-662.377] * (-667.043) [-665.917] (-673.883) (-673.520) -- 0:03:59 99000 -- (-673.968) (-662.457) (-659.914) [-666.649] * (-675.198) (-671.370) [-663.720] (-663.886) -- 0:03:56 100000 -- (-664.169) (-668.336) (-671.058) [-659.127] * (-669.196) [-668.832] (-671.555) (-667.832) -- 0:03:53 Average standard deviation of split frequencies: 0.026536 101000 -- (-665.570) [-668.308] (-670.768) (-666.280) * (-667.679) [-663.866] (-675.731) (-667.562) -- 0:03:51 102000 -- (-673.289) (-669.551) (-665.465) [-668.802] * (-671.509) (-664.863) [-663.405] (-669.068) -- 0:03:57 103000 -- (-670.857) (-671.880) [-669.476] (-661.167) * (-666.167) (-664.865) (-671.884) [-658.790] -- 0:03:55 104000 -- [-680.464] (-664.886) (-664.446) (-673.899) * (-667.835) (-665.257) (-663.958) [-668.461] -- 0:03:52 105000 -- (-670.385) (-668.861) [-670.770] (-661.187) * (-661.551) [-661.871] (-671.013) (-669.648) -- 0:03:58 Average standard deviation of split frequencies: 0.024055 106000 -- (-665.485) (-668.370) (-676.305) [-670.714] * [-667.161] (-665.594) (-671.153) (-663.530) -- 0:03:56 107000 -- (-659.705) [-662.942] (-679.043) (-665.572) * (-664.455) [-669.717] (-678.772) (-671.604) -- 0:03:53 108000 -- [-662.160] (-683.389) (-670.921) (-664.419) * (-669.301) (-678.889) [-668.137] (-674.925) -- 0:03:51 109000 -- (-666.335) (-669.865) (-671.477) [-666.088] * (-677.377) [-663.536] (-668.156) (-670.659) -- 0:03:57 110000 -- (-671.261) (-668.045) [-670.620] (-680.507) * (-668.518) [-660.984] (-661.729) (-671.808) -- 0:03:54 Average standard deviation of split frequencies: 0.023073 111000 -- (-668.780) (-661.527) (-675.899) [-668.374] * (-667.653) (-675.579) [-668.934] (-668.367) -- 0:03:52 112000 -- (-661.918) [-666.814] (-670.967) (-673.660) * (-669.872) [-679.641] (-672.398) (-665.493) -- 0:03:49 113000 -- [-670.879] (-665.410) (-668.563) (-667.291) * (-682.472) (-676.356) (-670.745) [-665.479] -- 0:03:55 114000 -- (-679.810) (-659.912) [-665.215] (-664.790) * (-665.459) (-666.086) (-667.262) [-664.899] -- 0:03:53 115000 -- (-669.731) (-669.725) (-673.981) [-667.188] * (-665.345) (-676.165) (-659.517) [-664.285] -- 0:03:50 Average standard deviation of split frequencies: 0.024044 116000 -- (-666.592) (-669.185) (-674.152) [-664.780] * (-667.226) (-667.110) [-660.599] (-661.627) -- 0:03:48 117000 -- (-664.585) (-676.457) [-667.795] (-670.213) * (-671.867) (-669.723) (-671.656) [-666.695] -- 0:03:53 118000 -- (-666.774) (-664.275) (-672.756) [-664.829] * (-664.908) (-670.358) [-660.086] (-663.841) -- 0:03:51 119000 -- [-666.717] (-669.709) (-670.387) (-672.505) * (-662.091) (-674.137) [-661.367] (-671.546) -- 0:03:49 120000 -- (-667.382) (-664.876) [-667.852] (-668.735) * [-659.232] (-665.182) (-668.846) (-661.093) -- 0:03:54 Average standard deviation of split frequencies: 0.022591 121000 -- (-668.761) (-663.284) (-664.934) [-666.227] * (-667.387) (-668.595) [-668.303] (-668.544) -- 0:03:52 122000 -- (-664.171) (-668.025) [-668.491] (-667.765) * (-675.286) [-673.761] (-675.966) (-666.152) -- 0:03:50 123000 -- (-667.740) [-669.282] (-681.516) (-661.763) * (-680.119) (-664.729) (-661.962) [-665.953] -- 0:03:48 124000 -- (-670.347) [-664.874] (-669.523) (-664.781) * [-665.504] (-661.777) (-667.305) (-663.199) -- 0:03:53 125000 -- [-669.026] (-669.785) (-673.399) (-662.705) * (-674.920) (-674.379) [-665.861] (-669.141) -- 0:03:51 Average standard deviation of split frequencies: 0.021357 126000 -- (-673.152) (-669.148) (-678.207) [-669.993] * (-664.510) [-667.904] (-671.351) (-662.683) -- 0:03:48 127000 -- (-668.295) (-663.331) [-668.617] (-671.759) * (-663.661) (-675.282) (-672.376) [-662.196] -- 0:03:46 128000 -- [-663.245] (-665.461) (-675.122) (-670.508) * (-670.096) [-665.279] (-676.268) (-667.705) -- 0:03:51 129000 -- (-663.507) (-674.853) (-665.053) [-664.689] * (-660.641) (-664.034) (-671.216) [-663.768] -- 0:03:49 130000 -- (-664.246) (-664.751) (-662.653) [-662.981] * [-665.303] (-670.133) (-679.577) (-665.398) -- 0:03:47 Average standard deviation of split frequencies: 0.022117 131000 -- (-666.539) (-660.419) [-666.257] (-664.082) * [-668.161] (-667.302) (-666.953) (-661.019) -- 0:03:45 132000 -- (-664.522) [-663.533] (-671.265) (-666.783) * (-662.536) (-671.872) [-665.278] (-663.918) -- 0:03:50 133000 -- (-669.701) (-663.052) [-670.527] (-659.360) * (-661.614) (-674.656) (-669.972) [-666.678] -- 0:03:48 134000 -- (-669.450) (-673.768) (-665.511) [-668.782] * (-667.708) [-659.854] (-662.195) (-666.432) -- 0:03:46 135000 -- (-671.157) (-668.033) (-669.093) [-663.240] * (-674.038) (-666.050) [-660.860] (-673.846) -- 0:03:50 Average standard deviation of split frequencies: 0.020345 136000 -- (-668.647) [-665.339] (-677.689) (-671.870) * [-662.828] (-671.301) (-673.108) (-672.971) -- 0:03:48 137000 -- [-665.090] (-662.231) (-664.517) (-675.114) * [-665.445] (-662.192) (-674.979) (-676.991) -- 0:03:46 138000 -- (-677.931) (-665.816) (-674.739) [-671.783] * (-668.004) (-663.567) (-663.024) [-660.854] -- 0:03:44 139000 -- (-672.983) [-671.924] (-662.555) (-668.337) * (-665.704) (-665.802) [-671.156] (-670.145) -- 0:03:49 140000 -- (-674.629) [-662.071] (-661.839) (-672.476) * [-671.413] (-672.560) (-665.125) (-679.456) -- 0:03:47 Average standard deviation of split frequencies: 0.019803 141000 -- (-675.046) [-668.367] (-659.776) (-672.703) * [-663.259] (-664.921) (-664.233) (-668.272) -- 0:03:45 142000 -- (-669.102) (-666.407) (-665.307) [-666.325] * [-663.383] (-666.005) (-663.788) (-670.782) -- 0:03:43 143000 -- (-669.797) (-668.509) (-664.907) [-666.494] * (-664.482) (-667.403) (-666.812) [-675.997] -- 0:03:47 144000 -- (-664.647) (-667.490) [-668.217] (-678.057) * (-668.234) [-663.215] (-675.870) (-668.938) -- 0:03:45 145000 -- [-672.442] (-676.623) (-666.768) (-666.958) * [-670.718] (-664.510) (-667.234) (-676.325) -- 0:03:44 Average standard deviation of split frequencies: 0.020180 146000 -- (-669.495) (-673.284) [-666.551] (-659.633) * (-671.478) (-672.505) [-668.417] (-670.828) -- 0:03:42 147000 -- [-665.815] (-663.834) (-673.266) (-658.701) * (-672.260) (-663.206) [-669.586] (-670.977) -- 0:03:46 148000 -- (-662.782) [-669.675] (-666.544) (-660.321) * [-664.568] (-665.090) (-682.828) (-664.023) -- 0:03:44 149000 -- (-673.868) (-668.327) (-664.058) [-667.940] * (-671.462) [-662.183] (-673.813) (-667.561) -- 0:03:42 150000 -- (-667.888) [-663.713] (-666.238) (-675.267) * (-665.288) [-660.865] (-668.518) (-670.160) -- 0:03:46 Average standard deviation of split frequencies: 0.019425 151000 -- (-663.983) [-665.418] (-664.672) (-670.102) * (-667.294) (-666.789) [-661.588] (-675.118) -- 0:03:44 152000 -- (-673.543) (-670.060) [-660.836] (-668.500) * (-664.201) [-675.726] (-670.853) (-667.456) -- 0:03:43 153000 -- (-666.855) [-661.740] (-665.969) (-676.862) * [-659.443] (-667.673) (-668.658) (-664.393) -- 0:03:41 154000 -- (-665.781) [-663.119] (-665.394) (-668.419) * [-666.193] (-666.704) (-668.180) (-666.588) -- 0:03:45 155000 -- (-664.808) [-666.536] (-670.292) (-669.109) * (-657.820) (-664.712) (-671.739) [-660.908] -- 0:03:43 Average standard deviation of split frequencies: 0.019581 156000 -- [-671.389] (-665.592) (-665.911) (-671.118) * (-664.795) (-659.957) [-664.637] (-665.204) -- 0:03:41 157000 -- (-666.616) (-668.511) (-668.104) [-668.222] * (-666.866) (-663.907) [-665.865] (-668.360) -- 0:03:40 158000 -- (-664.914) (-665.189) (-667.360) [-667.043] * (-666.066) (-659.644) [-659.668] (-673.088) -- 0:03:43 159000 -- (-674.454) (-664.458) (-673.607) [-664.370] * (-666.390) (-660.505) (-661.364) [-662.366] -- 0:03:42 160000 -- (-671.889) (-672.809) (-664.996) [-668.301] * (-667.432) (-666.305) (-665.826) [-665.047] -- 0:03:40 Average standard deviation of split frequencies: 0.018093 161000 -- [-666.348] (-669.329) (-674.154) (-663.578) * [-662.790] (-660.156) (-662.846) (-660.620) -- 0:03:38 162000 -- (-671.722) (-662.893) (-665.509) [-669.629] * (-664.216) (-669.035) (-668.627) [-663.724] -- 0:03:42 163000 -- (-668.024) (-676.126) (-668.742) [-668.045] * [-660.030] (-673.949) (-663.217) (-670.555) -- 0:03:40 164000 -- (-672.331) (-666.245) (-662.385) [-673.425] * (-658.986) (-667.993) [-666.076] (-665.715) -- 0:03:39 165000 -- (-672.218) [-664.602] (-672.307) (-672.707) * [-663.870] (-671.713) (-664.878) (-672.049) -- 0:03:37 Average standard deviation of split frequencies: 0.018240 166000 -- (-665.763) (-665.323) (-669.828) [-658.500] * (-668.771) (-665.876) (-664.249) [-669.140] -- 0:03:41 167000 -- [-667.159] (-659.712) (-674.134) (-672.656) * [-661.015] (-666.584) (-663.371) (-673.058) -- 0:03:39 168000 -- (-669.757) (-670.283) (-676.041) [-667.715] * (-665.431) (-668.396) [-667.655] (-672.113) -- 0:03:37 169000 -- (-664.543) [-668.563] (-665.057) (-664.219) * (-666.637) (-665.541) (-663.888) [-672.660] -- 0:03:41 170000 -- (-664.971) (-671.419) [-662.846] (-669.862) * (-663.648) (-665.643) [-661.294] (-671.302) -- 0:03:39 Average standard deviation of split frequencies: 0.018069 171000 -- (-665.689) (-667.228) (-667.973) [-661.913] * [-669.511] (-667.869) (-676.805) (-671.645) -- 0:03:38 172000 -- (-667.915) [-662.239] (-670.177) (-665.949) * (-669.614) [-663.102] (-662.107) (-668.154) -- 0:03:36 173000 -- [-663.823] (-669.972) (-668.185) (-666.139) * (-674.643) (-670.410) (-673.274) [-667.354] -- 0:03:39 174000 -- (-669.020) [-664.798] (-671.871) (-665.024) * [-665.442] (-668.438) (-675.682) (-670.126) -- 0:03:38 175000 -- (-666.138) [-661.695] (-662.722) (-684.369) * (-673.106) [-666.421] (-669.154) (-674.363) -- 0:03:36 Average standard deviation of split frequencies: 0.017856 176000 -- [-665.514] (-667.811) (-668.854) (-667.016) * (-668.543) (-670.359) [-664.705] (-665.056) -- 0:03:35 177000 -- (-673.736) (-661.979) (-681.303) [-659.318] * (-677.593) [-664.542] (-665.966) (-665.449) -- 0:03:38 178000 -- [-663.277] (-670.049) (-673.664) (-656.863) * [-668.248] (-665.237) (-661.152) (-668.123) -- 0:03:37 179000 -- (-669.577) (-665.090) [-663.808] (-670.459) * (-667.739) (-660.786) [-662.386] (-669.541) -- 0:03:35 180000 -- [-665.756] (-670.947) (-664.859) (-667.099) * (-667.122) [-665.188] (-663.583) (-669.058) -- 0:03:34 Average standard deviation of split frequencies: 0.015542 181000 -- (-674.504) (-664.763) [-663.915] (-679.057) * (-671.327) [-666.550] (-666.322) (-666.726) -- 0:03:37 182000 -- [-665.642] (-671.445) (-661.573) (-666.027) * (-668.743) [-662.376] (-669.712) (-668.132) -- 0:03:35 183000 -- (-669.077) (-666.449) (-678.615) [-667.029] * (-670.783) [-659.844] (-661.411) (-675.061) -- 0:03:34 184000 -- (-669.692) (-662.907) [-663.724] (-668.718) * (-668.507) (-664.418) [-670.264] (-673.976) -- 0:03:37 185000 -- (-665.532) [-667.760] (-664.287) (-666.273) * [-672.748] (-665.720) (-668.123) (-670.396) -- 0:03:35 Average standard deviation of split frequencies: 0.016423 186000 -- (-665.848) [-669.476] (-667.023) (-671.607) * [-661.165] (-673.873) (-674.036) (-673.012) -- 0:03:34 187000 -- [-665.259] (-664.076) (-668.373) (-666.224) * (-674.799) [-666.048] (-670.007) (-663.981) -- 0:03:33 188000 -- (-667.410) [-663.325] (-663.943) (-663.287) * (-676.040) (-661.443) (-685.103) [-662.657] -- 0:03:35 189000 -- (-671.927) (-670.680) (-671.781) [-661.087] * [-665.604] (-669.801) (-668.535) (-683.479) -- 0:03:34 190000 -- (-668.900) (-668.717) (-673.975) [-666.957] * (-668.215) (-665.090) [-668.890] (-669.009) -- 0:03:33 Average standard deviation of split frequencies: 0.016174 191000 -- (-663.187) [-659.777] (-661.545) (-672.949) * (-668.691) (-671.696) [-665.787] (-668.916) -- 0:03:31 192000 -- [-665.023] (-665.360) (-665.979) (-679.061) * (-663.546) [-675.243] (-665.028) (-664.819) -- 0:03:34 193000 -- (-670.954) (-664.490) (-666.526) [-665.624] * (-661.900) (-664.588) [-665.653] (-675.335) -- 0:03:33 194000 -- [-663.261] (-664.540) (-666.402) (-668.104) * (-664.942) (-667.981) (-661.727) [-663.644] -- 0:03:31 195000 -- (-668.977) [-662.452] (-668.380) (-667.187) * (-680.627) (-663.157) (-665.420) [-665.676] -- 0:03:34 Average standard deviation of split frequencies: 0.016208 196000 -- (-670.200) (-667.240) (-665.126) [-664.665] * [-668.576] (-674.606) (-670.788) (-667.258) -- 0:03:33 197000 -- (-668.664) (-663.521) (-669.457) [-662.085] * (-666.351) (-662.342) [-662.406] (-668.472) -- 0:03:31 198000 -- (-665.674) (-661.557) (-674.207) [-661.567] * [-659.405] (-671.107) (-668.620) (-666.098) -- 0:03:30 199000 -- (-668.634) [-664.285] (-668.746) (-665.046) * [-668.222] (-670.043) (-668.018) (-659.634) -- 0:03:33 200000 -- (-664.518) (-660.433) [-663.828] (-659.987) * [-663.470] (-665.107) (-665.404) (-667.270) -- 0:03:32 Average standard deviation of split frequencies: 0.016138 201000 -- (-664.116) (-665.615) (-664.925) [-665.404] * (-672.415) (-672.055) (-667.355) [-666.673] -- 0:03:30 202000 -- (-666.046) [-666.417] (-665.401) (-668.737) * [-672.727] (-677.623) (-662.686) (-668.438) -- 0:03:29 203000 -- (-668.028) [-663.668] (-668.379) (-662.294) * (-679.083) (-669.756) [-672.570] (-670.449) -- 0:03:32 204000 -- (-661.947) (-665.127) [-663.302] (-677.102) * (-666.342) (-669.840) [-665.736] (-665.371) -- 0:03:30 205000 -- (-671.174) (-664.703) [-667.714] (-677.326) * (-662.667) [-659.783] (-680.059) (-678.703) -- 0:03:29 Average standard deviation of split frequencies: 0.016118 206000 -- (-665.448) [-662.686] (-662.168) (-667.376) * [-659.131] (-665.224) (-665.528) (-671.290) -- 0:03:28 207000 -- (-663.500) [-665.114] (-665.561) (-668.292) * [-665.252] (-663.837) (-676.795) (-663.824) -- 0:03:30 208000 -- (-668.638) (-669.234) [-662.239] (-671.987) * [-669.244] (-664.956) (-674.732) (-667.099) -- 0:03:29 209000 -- (-660.313) (-667.834) [-660.320] (-671.883) * (-668.903) (-663.200) (-672.504) [-666.951] -- 0:03:28 210000 -- [-669.515] (-671.984) (-671.003) (-680.021) * [-673.145] (-666.757) (-665.224) (-673.021) -- 0:03:26 Average standard deviation of split frequencies: 0.014885 211000 -- (-685.570) (-670.580) (-669.260) [-669.902] * (-666.992) (-663.447) [-662.968] (-666.287) -- 0:03:29 212000 -- (-664.366) (-675.528) (-665.270) [-662.984] * (-672.821) [-664.322] (-671.492) (-668.579) -- 0:03:28 213000 -- (-672.010) (-681.370) [-662.428] (-678.676) * (-666.317) [-664.831] (-666.467) (-672.095) -- 0:03:26 214000 -- (-674.907) (-663.088) (-668.169) [-663.833] * [-665.881] (-670.232) (-664.291) (-673.348) -- 0:03:29 215000 -- (-667.725) [-666.783] (-667.744) (-662.866) * (-668.266) (-664.744) [-665.047] (-677.343) -- 0:03:28 Average standard deviation of split frequencies: 0.014897 216000 -- (-670.814) [-662.996] (-665.532) (-661.911) * [-662.130] (-663.512) (-664.393) (-664.304) -- 0:03:26 217000 -- (-665.034) (-657.962) [-665.697] (-667.909) * (-662.679) (-665.498) (-668.964) [-671.795] -- 0:03:25 218000 -- (-676.615) [-661.624] (-667.351) (-672.604) * (-672.153) [-665.031] (-667.477) (-673.275) -- 0:03:28 219000 -- (-672.828) (-679.748) [-663.993] (-669.827) * (-665.183) [-662.617] (-668.240) (-673.446) -- 0:03:26 220000 -- [-666.377] (-675.558) (-663.288) (-670.819) * (-666.383) (-669.845) [-661.275] (-674.371) -- 0:03:25 Average standard deviation of split frequencies: 0.015925 221000 -- (-672.705) (-674.452) [-667.753] (-672.521) * (-678.069) [-680.126] (-663.963) (-672.148) -- 0:03:27 222000 -- (-663.723) (-667.115) [-676.688] (-668.649) * (-676.732) [-672.110] (-665.857) (-669.919) -- 0:03:26 223000 -- (-660.940) (-667.937) [-667.940] (-669.444) * (-671.251) [-668.106] (-663.473) (-666.038) -- 0:03:25 224000 -- (-673.257) (-670.329) (-666.823) [-666.616] * [-667.852] (-669.079) (-677.889) (-671.570) -- 0:03:24 225000 -- (-675.242) (-665.987) [-661.673] (-675.991) * (-671.495) (-661.580) [-670.957] (-666.951) -- 0:03:26 Average standard deviation of split frequencies: 0.013843 226000 -- (-669.513) [-665.645] (-676.616) (-668.745) * (-664.915) (-673.085) [-666.112] (-664.522) -- 0:03:25 227000 -- (-665.667) [-671.789] (-666.692) (-663.471) * (-668.547) (-669.242) [-665.908] (-662.076) -- 0:03:24 228000 -- (-665.637) [-666.476] (-663.724) (-670.100) * (-670.233) [-662.151] (-666.933) (-671.706) -- 0:03:23 229000 -- (-666.587) (-674.097) (-663.944) [-670.930] * [-671.951] (-666.436) (-666.626) (-664.820) -- 0:03:25 230000 -- (-663.870) [-664.560] (-663.657) (-664.663) * (-662.463) [-660.430] (-665.817) (-665.845) -- 0:03:24 Average standard deviation of split frequencies: 0.013934 231000 -- (-664.149) (-675.067) [-659.484] (-671.669) * (-666.075) (-668.096) (-662.689) [-665.267] -- 0:03:23 232000 -- (-660.327) (-665.134) [-670.487] (-673.228) * (-675.632) (-668.202) (-663.133) [-669.859] -- 0:03:25 233000 -- (-670.238) (-666.525) (-663.499) [-666.794] * (-666.666) (-669.807) [-666.510] (-668.597) -- 0:03:24 234000 -- (-663.561) (-675.250) [-662.667] (-663.814) * (-671.376) [-665.258] (-662.662) (-669.720) -- 0:03:22 235000 -- (-675.292) (-678.644) [-661.957] (-663.726) * (-676.132) [-667.419] (-671.187) (-672.692) -- 0:03:21 Average standard deviation of split frequencies: 0.013438 236000 -- [-660.799] (-668.004) (-668.566) (-666.470) * (-669.064) (-670.441) [-657.292] (-672.622) -- 0:03:23 237000 -- (-669.878) (-666.838) (-668.480) [-664.337] * (-665.307) (-661.000) [-664.872] (-661.462) -- 0:03:22 238000 -- [-669.884] (-661.485) (-667.324) (-667.120) * (-682.610) (-663.335) [-662.891] (-663.765) -- 0:03:21 239000 -- [-663.639] (-667.206) (-666.092) (-680.246) * (-679.896) [-668.738] (-662.086) (-670.647) -- 0:03:23 240000 -- [-667.162] (-663.912) (-667.149) (-666.464) * (-667.085) [-662.876] (-663.044) (-670.664) -- 0:03:22 Average standard deviation of split frequencies: 0.014067 241000 -- (-669.011) (-669.623) [-660.293] (-669.520) * (-667.966) [-665.267] (-668.197) (-671.198) -- 0:03:21 242000 -- (-665.763) (-663.675) (-663.903) [-663.883] * (-671.574) [-672.085] (-669.527) (-664.648) -- 0:03:20 243000 -- (-667.356) (-663.008) (-666.838) [-667.011] * [-659.365] (-665.466) (-670.028) (-666.848) -- 0:03:22 244000 -- (-667.466) [-662.383] (-669.309) (-671.449) * [-671.071] (-664.677) (-672.061) (-669.617) -- 0:03:21 245000 -- [-657.695] (-674.043) (-669.366) (-666.733) * [-662.605] (-666.459) (-674.662) (-661.501) -- 0:03:20 Average standard deviation of split frequencies: 0.014111 246000 -- [-671.705] (-676.968) (-674.622) (-671.891) * (-664.350) [-665.501] (-670.701) (-673.103) -- 0:03:19 247000 -- (-665.246) (-666.934) (-674.805) [-663.021] * (-671.892) (-671.006) [-672.895] (-670.528) -- 0:03:21 248000 -- (-665.314) [-663.242] (-664.374) (-667.426) * (-667.819) (-680.287) [-672.389] (-662.537) -- 0:03:20 249000 -- (-665.224) [-663.674] (-668.939) (-667.315) * [-675.579] (-678.578) (-667.581) (-663.775) -- 0:03:19 250000 -- [-665.095] (-669.459) (-674.369) (-663.390) * (-667.804) (-660.160) [-677.232] (-664.173) -- 0:03:18 Average standard deviation of split frequencies: 0.014275 251000 -- (-677.890) (-665.645) [-663.614] (-665.553) * (-669.526) (-663.929) (-667.897) [-667.124] -- 0:03:19 252000 -- (-667.349) (-670.868) [-666.857] (-665.830) * (-679.878) [-665.283] (-668.140) (-666.367) -- 0:03:18 253000 -- [-666.572] (-666.598) (-668.402) (-672.377) * (-669.288) (-667.112) (-668.479) [-665.955] -- 0:03:17 254000 -- (-664.399) [-665.088] (-661.963) (-674.117) * (-666.071) (-664.566) [-665.154] (-672.109) -- 0:03:19 255000 -- (-669.734) (-675.255) (-662.330) [-666.698] * (-675.691) (-667.166) [-664.156] (-664.176) -- 0:03:18 Average standard deviation of split frequencies: 0.014145 256000 -- [-666.279] (-660.412) (-661.084) (-671.671) * (-676.065) [-664.398] (-667.897) (-670.664) -- 0:03:17 257000 -- (-663.978) [-665.420] (-669.065) (-672.254) * [-664.573] (-675.006) (-671.037) (-664.705) -- 0:03:16 258000 -- (-669.223) (-672.606) [-664.293] (-667.855) * (-665.017) [-670.341] (-668.025) (-665.766) -- 0:03:18 259000 -- (-676.744) (-667.172) [-659.021] (-671.169) * (-678.453) (-661.655) (-669.092) [-668.514] -- 0:03:17 260000 -- (-674.037) [-671.555] (-661.499) (-672.106) * (-675.828) (-659.649) [-661.098] (-670.674) -- 0:03:16 Average standard deviation of split frequencies: 0.013646 261000 -- (-666.251) (-670.284) (-670.063) [-668.242] * (-662.242) (-666.490) (-669.479) [-673.086] -- 0:03:15 262000 -- (-670.218) [-667.595] (-666.195) (-665.374) * (-664.723) (-663.709) [-661.421] (-663.787) -- 0:03:17 263000 -- (-670.927) [-662.864] (-665.935) (-675.926) * (-668.112) (-670.450) (-675.807) [-666.808] -- 0:03:16 264000 -- (-669.199) [-670.546] (-663.518) (-663.818) * (-665.713) [-673.019] (-675.070) (-663.787) -- 0:03:15 265000 -- (-665.873) [-673.575] (-664.296) (-675.923) * [-663.312] (-664.511) (-670.910) (-666.452) -- 0:03:16 Average standard deviation of split frequencies: 0.012945 266000 -- (-668.595) [-665.363] (-665.339) (-665.699) * (-666.488) [-665.475] (-668.836) (-665.915) -- 0:03:15 267000 -- (-667.579) [-664.106] (-667.362) (-674.756) * (-669.801) (-673.588) (-673.458) [-662.540] -- 0:03:14 268000 -- [-663.239] (-667.691) (-667.697) (-663.028) * (-668.609) (-674.053) [-659.607] (-668.599) -- 0:03:13 269000 -- (-667.673) (-663.601) (-663.490) [-661.806] * (-666.377) (-665.033) (-664.970) [-668.738] -- 0:03:15 270000 -- (-663.330) [-668.593] (-677.886) (-661.537) * (-663.854) [-663.260] (-665.419) (-673.600) -- 0:03:14 Average standard deviation of split frequencies: 0.013706 271000 -- (-672.606) (-680.660) (-667.919) [-661.696] * (-664.241) (-671.283) (-671.777) [-668.668] -- 0:03:13 272000 -- (-676.511) (-663.414) [-667.028] (-671.138) * (-664.922) (-668.129) (-671.857) [-665.989] -- 0:03:12 273000 -- (-673.781) [-669.260] (-669.798) (-667.683) * (-667.638) [-664.282] (-666.138) (-668.562) -- 0:03:14 274000 -- (-676.058) (-661.574) [-669.900] (-664.482) * (-663.097) [-667.082] (-670.763) (-662.818) -- 0:03:13 275000 -- [-666.958] (-675.372) (-665.038) (-670.272) * (-662.242) [-668.211] (-668.569) (-671.791) -- 0:03:12 Average standard deviation of split frequencies: 0.013339 276000 -- (-671.562) (-667.179) [-664.819] (-673.542) * [-669.704] (-672.471) (-667.403) (-669.540) -- 0:03:11 277000 -- [-666.994] (-666.115) (-673.544) (-664.716) * (-675.932) [-661.643] (-682.715) (-665.717) -- 0:03:13 278000 -- (-668.407) (-672.819) (-671.742) [-665.411] * (-673.454) (-663.013) (-667.874) [-666.418] -- 0:03:12 279000 -- (-659.836) (-665.352) (-665.012) [-662.848] * [-669.655] (-666.447) (-662.154) (-669.106) -- 0:03:11 280000 -- [-661.214] (-668.789) (-669.460) (-675.901) * (-666.255) [-667.595] (-669.263) (-666.170) -- 0:03:12 Average standard deviation of split frequencies: 0.011589 281000 -- (-665.674) (-661.999) [-670.408] (-674.420) * [-665.808] (-670.556) (-670.847) (-660.369) -- 0:03:11 282000 -- [-667.047] (-664.758) (-673.334) (-666.210) * (-670.962) (-667.047) [-668.160] (-667.469) -- 0:03:10 283000 -- (-667.645) (-664.745) [-657.997] (-665.623) * (-668.355) (-673.334) [-662.813] (-668.274) -- 0:03:10 284000 -- (-669.282) [-670.984] (-669.003) (-673.180) * (-663.698) (-665.484) [-671.003] (-670.082) -- 0:03:11 285000 -- (-667.298) (-669.245) (-670.541) [-674.965] * [-665.336] (-668.301) (-680.709) (-664.561) -- 0:03:10 Average standard deviation of split frequencies: 0.012512 286000 -- (-668.656) (-669.803) (-667.415) [-666.980] * [-665.931] (-674.568) (-679.564) (-663.798) -- 0:03:09 287000 -- [-666.715] (-664.850) (-668.658) (-681.059) * (-669.042) (-668.764) [-665.200] (-664.408) -- 0:03:08 288000 -- (-675.049) (-673.390) (-663.127) [-665.732] * (-678.853) (-664.680) [-667.730] (-667.751) -- 0:03:10 289000 -- (-669.590) (-667.221) (-663.984) [-664.769] * [-672.693] (-668.114) (-667.819) (-672.237) -- 0:03:09 290000 -- (-667.097) [-664.845] (-665.863) (-673.417) * [-666.569] (-661.048) (-678.589) (-668.042) -- 0:03:08 Average standard deviation of split frequencies: 0.011275 291000 -- (-673.096) [-668.850] (-670.038) (-662.575) * [-672.505] (-666.621) (-685.334) (-671.297) -- 0:03:10 292000 -- [-663.854] (-671.029) (-670.805) (-659.090) * (-667.551) [-659.577] (-670.501) (-664.201) -- 0:03:09 293000 -- (-664.745) (-680.518) (-671.163) [-670.278] * (-666.790) (-670.105) [-662.054] (-676.007) -- 0:03:08 294000 -- (-671.741) (-665.094) [-664.459] (-659.401) * [-662.991] (-674.010) (-660.714) (-666.915) -- 0:03:07 295000 -- (-673.153) (-667.427) [-663.456] (-669.125) * [-667.532] (-678.621) (-668.500) (-667.361) -- 0:03:08 Average standard deviation of split frequencies: 0.011527 296000 -- (-661.409) (-666.211) [-662.699] (-665.047) * (-667.589) (-669.306) [-673.727] (-661.956) -- 0:03:07 297000 -- (-678.235) [-666.007] (-661.156) (-668.452) * (-670.660) (-663.216) (-666.706) [-678.983] -- 0:03:06 298000 -- (-663.039) [-662.390] (-673.664) (-667.907) * (-663.347) [-664.908] (-667.898) (-671.037) -- 0:03:06 299000 -- (-663.637) (-674.971) (-672.652) [-664.980] * (-667.395) (-675.230) [-668.677] (-666.591) -- 0:03:07 300000 -- (-661.281) (-666.324) [-667.995] (-668.968) * (-679.546) (-669.946) (-662.878) [-667.413] -- 0:03:06 Average standard deviation of split frequencies: 0.011572 301000 -- (-673.652) [-664.475] (-668.115) (-682.075) * (-675.101) (-667.261) [-666.398] (-665.652) -- 0:03:05 302000 -- [-663.669] (-671.451) (-677.106) (-657.539) * (-674.019) (-670.490) (-668.520) [-670.549] -- 0:03:04 303000 -- [-661.496] (-666.011) (-669.552) (-667.056) * [-665.353] (-665.967) (-667.606) (-672.493) -- 0:03:06 304000 -- [-662.004] (-669.291) (-674.655) (-664.423) * (-661.520) (-663.235) [-664.493] (-672.348) -- 0:03:05 305000 -- (-664.415) (-668.992) (-676.317) [-667.511] * (-665.801) (-674.706) [-669.114] (-665.221) -- 0:03:04 Average standard deviation of split frequencies: 0.012324 306000 -- (-667.308) (-672.702) (-665.125) [-665.141] * [-668.544] (-661.804) (-665.355) (-670.644) -- 0:03:05 307000 -- (-667.044) (-665.889) (-666.258) [-672.920] * [-662.205] (-666.791) (-662.579) (-676.003) -- 0:03:05 308000 -- (-670.551) (-664.898) (-667.045) [-670.417] * [-661.054] (-665.016) (-666.408) (-668.619) -- 0:03:04 309000 -- (-669.763) (-673.479) (-674.878) [-666.154] * (-667.159) [-664.035] (-665.474) (-666.934) -- 0:03:03 310000 -- (-666.431) (-665.194) (-675.960) [-665.394] * (-672.365) (-668.333) [-670.863] (-670.850) -- 0:03:04 Average standard deviation of split frequencies: 0.011778 311000 -- (-668.708) [-664.742] (-668.539) (-673.228) * (-664.140) (-670.722) [-665.629] (-672.871) -- 0:03:03 312000 -- [-661.133] (-668.929) (-669.661) (-676.984) * [-664.945] (-668.731) (-668.157) (-676.308) -- 0:03:03 313000 -- (-669.036) (-675.355) [-667.181] (-661.981) * (-677.175) (-667.925) (-672.121) [-672.788] -- 0:03:02 314000 -- (-664.247) [-662.153] (-663.952) (-666.908) * (-667.241) [-664.948] (-674.509) (-676.732) -- 0:03:03 315000 -- (-660.736) (-674.253) [-667.421] (-670.884) * [-674.896] (-667.463) (-672.126) (-672.486) -- 0:03:02 Average standard deviation of split frequencies: 0.012716 316000 -- (-671.926) (-666.952) [-665.420] (-669.293) * [-681.321] (-671.754) (-669.313) (-675.442) -- 0:03:01 317000 -- (-667.606) (-669.415) (-665.424) [-664.493] * [-659.184] (-665.253) (-674.771) (-669.877) -- 0:03:00 318000 -- (-658.362) (-679.793) (-665.817) [-673.150] * (-669.352) (-679.200) (-669.939) [-669.186] -- 0:03:02 319000 -- [-665.004] (-671.866) (-664.919) (-672.022) * (-664.628) (-670.369) (-668.229) [-663.432] -- 0:03:01 320000 -- (-668.570) [-676.341] (-669.939) (-666.229) * [-667.914] (-672.700) (-673.960) (-667.067) -- 0:03:00 Average standard deviation of split frequencies: 0.011971 321000 -- (-671.798) [-668.205] (-672.644) (-666.556) * (-662.150) (-669.119) (-673.426) [-656.622] -- 0:03:01 322000 -- [-667.039] (-676.677) (-672.840) (-679.476) * (-670.764) (-667.929) (-674.964) [-663.877] -- 0:03:01 323000 -- [-664.001] (-670.674) (-667.296) (-662.962) * [-672.297] (-664.536) (-667.374) (-672.273) -- 0:03:00 324000 -- [-664.386] (-667.494) (-663.023) (-678.346) * (-668.330) (-666.175) (-672.236) [-658.743] -- 0:02:59 325000 -- (-667.375) (-674.291) (-663.759) [-659.938] * [-663.420] (-664.643) (-672.031) (-666.997) -- 0:03:00 Average standard deviation of split frequencies: 0.011568 326000 -- (-660.639) [-664.365] (-667.180) (-678.220) * [-669.296] (-673.131) (-666.225) (-677.807) -- 0:02:59 327000 -- (-671.892) [-663.768] (-668.856) (-669.348) * [-668.523] (-663.358) (-676.214) (-672.490) -- 0:02:59 328000 -- (-668.542) (-667.453) [-666.994] (-664.653) * [-667.746] (-675.448) (-672.623) (-668.603) -- 0:02:58 329000 -- [-664.272] (-667.685) (-666.736) (-667.239) * (-677.072) (-672.249) [-669.186] (-669.371) -- 0:02:59 330000 -- [-664.391] (-664.617) (-675.781) (-666.750) * (-688.183) (-672.112) (-660.251) [-670.210] -- 0:02:58 Average standard deviation of split frequencies: 0.011599 331000 -- (-663.746) [-664.096] (-673.053) (-671.793) * [-663.138] (-662.895) (-672.360) (-662.606) -- 0:02:57 332000 -- (-666.279) (-666.286) (-669.880) [-665.588] * (-662.779) [-665.674] (-675.522) (-666.019) -- 0:02:57 333000 -- (-670.519) (-666.125) [-669.480] (-662.343) * (-664.682) (-668.275) (-669.350) [-671.330] -- 0:02:58 334000 -- [-665.010] (-673.873) (-663.991) (-668.403) * (-664.778) (-670.801) (-664.549) [-670.773] -- 0:02:57 335000 -- [-658.863] (-671.567) (-659.124) (-664.323) * (-662.750) [-662.299] (-674.557) (-665.611) -- 0:02:56 Average standard deviation of split frequencies: 0.011825 336000 -- (-668.669) (-669.331) (-664.606) [-666.589] * (-663.691) (-665.613) [-664.865] (-666.747) -- 0:02:57 337000 -- (-665.601) [-672.492] (-664.770) (-668.371) * (-681.797) (-670.994) (-665.765) [-667.393] -- 0:02:57 338000 -- (-669.188) [-664.826] (-671.647) (-671.338) * (-670.885) (-677.829) [-664.518] (-665.284) -- 0:02:56 339000 -- (-673.678) [-663.705] (-668.368) (-675.648) * (-672.467) (-674.790) [-666.490] (-678.720) -- 0:02:55 340000 -- (-676.582) (-662.843) [-676.203] (-672.458) * [-673.611] (-665.499) (-665.109) (-671.183) -- 0:02:56 Average standard deviation of split frequencies: 0.011400 341000 -- (-668.351) (-676.317) [-668.394] (-672.453) * [-671.476] (-667.886) (-669.935) (-667.473) -- 0:02:55 342000 -- [-662.988] (-666.668) (-674.935) (-668.691) * (-666.490) [-664.718] (-674.130) (-664.720) -- 0:02:55 343000 -- (-675.812) (-671.906) [-666.716] (-671.162) * (-666.037) (-670.663) (-660.718) [-659.928] -- 0:02:54 344000 -- (-660.161) [-664.854] (-673.900) (-683.549) * (-663.909) (-667.571) [-665.893] (-673.323) -- 0:02:55 345000 -- [-668.556] (-674.966) (-664.235) (-662.520) * [-667.735] (-663.852) (-665.258) (-667.080) -- 0:02:54 Average standard deviation of split frequencies: 0.010961 346000 -- (-667.455) [-667.671] (-668.903) (-665.234) * (-662.477) (-665.396) [-668.776] (-668.788) -- 0:02:53 347000 -- (-668.865) [-664.848] (-674.470) (-675.144) * (-667.242) (-669.991) [-665.597] (-664.673) -- 0:02:55 348000 -- (-665.378) (-670.822) (-661.183) [-674.208] * (-666.465) (-665.370) (-669.553) [-661.069] -- 0:02:54 349000 -- [-666.644] (-662.629) (-667.623) (-673.467) * (-663.592) (-674.688) [-667.256] (-670.503) -- 0:02:53 350000 -- (-663.802) (-673.245) (-669.187) [-669.096] * (-667.190) [-668.176] (-668.671) (-662.271) -- 0:02:52 Average standard deviation of split frequencies: 0.011203 351000 -- (-669.224) [-663.496] (-669.242) (-672.340) * (-666.316) [-666.807] (-673.351) (-666.718) -- 0:02:53 352000 -- [-664.215] (-664.092) (-672.828) (-674.298) * (-659.765) (-670.504) [-664.202] (-663.410) -- 0:02:53 353000 -- (-669.754) (-660.508) [-669.033] (-661.829) * (-666.051) (-661.637) [-666.460] (-663.220) -- 0:02:52 354000 -- (-673.107) (-664.688) [-667.159] (-666.267) * (-671.629) [-661.661] (-667.411) (-658.814) -- 0:02:51 355000 -- (-666.221) (-663.054) (-668.996) [-666.803] * (-664.214) (-661.760) (-669.269) [-668.528] -- 0:02:52 Average standard deviation of split frequencies: 0.010909 356000 -- (-669.562) [-662.002] (-665.986) (-668.907) * (-662.241) (-670.315) (-677.570) [-670.123] -- 0:02:51 357000 -- (-669.925) (-667.833) (-666.461) [-663.188] * [-666.884] (-670.848) (-673.655) (-669.665) -- 0:02:51 358000 -- [-665.580] (-669.877) (-676.793) (-673.970) * (-674.862) (-674.775) (-661.643) [-666.999] -- 0:02:50 359000 -- (-663.915) (-673.693) (-667.121) [-664.250] * (-672.989) [-666.656] (-667.243) (-665.444) -- 0:02:51 360000 -- (-665.070) [-662.177] (-665.226) (-671.252) * [-670.755] (-670.632) (-669.370) (-668.462) -- 0:02:50 Average standard deviation of split frequencies: 0.010145 361000 -- [-666.403] (-680.249) (-672.846) (-682.073) * [-662.382] (-667.522) (-675.906) (-668.616) -- 0:02:49 362000 -- (-665.247) (-665.538) [-660.945] (-671.497) * (-659.341) [-662.966] (-675.022) (-673.993) -- 0:02:50 363000 -- (-673.125) (-669.678) [-672.061] (-661.970) * [-669.768] (-673.398) (-667.531) (-666.916) -- 0:02:50 364000 -- (-672.033) (-673.285) [-674.246] (-671.989) * (-672.670) [-660.693] (-666.604) (-668.122) -- 0:02:49 365000 -- (-669.551) (-668.877) (-663.035) [-670.872] * [-664.080] (-669.690) (-669.893) (-664.550) -- 0:02:48 Average standard deviation of split frequencies: 0.010480 366000 -- (-667.532) [-664.240] (-681.219) (-668.520) * (-669.934) [-665.904] (-665.819) (-681.959) -- 0:02:49 367000 -- (-667.664) (-675.915) [-671.562] (-663.139) * (-666.115) [-665.671] (-669.423) (-667.223) -- 0:02:49 368000 -- [-662.358] (-667.262) (-683.152) (-672.585) * [-669.344] (-666.543) (-666.789) (-667.088) -- 0:02:48 369000 -- (-666.472) (-663.567) [-673.000] (-674.285) * (-661.848) [-664.820] (-671.680) (-667.550) -- 0:02:47 370000 -- (-668.759) (-667.003) (-673.131) [-669.376] * (-661.917) (-662.861) [-662.207] (-669.632) -- 0:02:48 Average standard deviation of split frequencies: 0.009205 371000 -- [-664.161] (-670.437) (-673.856) (-669.307) * (-666.797) (-670.299) [-665.422] (-670.599) -- 0:02:47 372000 -- (-667.178) (-674.881) [-662.944] (-672.840) * (-663.786) (-661.904) [-665.667] (-662.412) -- 0:02:47 373000 -- (-672.382) (-670.674) [-667.510] (-664.641) * (-668.084) (-665.588) [-666.620] (-666.156) -- 0:02:46 374000 -- (-675.023) (-672.813) (-662.202) [-662.624] * (-674.304) [-664.207] (-668.207) (-666.314) -- 0:02:47 375000 -- (-664.601) [-664.465] (-669.224) (-668.686) * (-667.853) (-672.996) (-670.264) [-671.537] -- 0:02:46 Average standard deviation of split frequencies: 0.008896 376000 -- (-660.144) [-664.664] (-661.770) (-665.737) * (-683.024) [-668.197] (-666.024) (-672.103) -- 0:02:45 377000 -- (-672.974) (-658.451) (-673.536) [-663.628] * [-665.757] (-672.003) (-671.763) (-665.252) -- 0:02:46 378000 -- (-671.105) (-661.924) (-672.087) [-661.479] * (-672.411) (-680.244) [-669.022] (-664.672) -- 0:02:46 379000 -- (-668.656) (-666.840) [-663.185] (-670.428) * (-661.440) (-666.526) [-666.195] (-666.773) -- 0:02:45 380000 -- (-664.778) (-666.637) [-665.415] (-664.938) * (-670.772) [-663.014] (-662.934) (-665.357) -- 0:02:44 Average standard deviation of split frequencies: 0.007926 381000 -- [-669.530] (-668.508) (-666.150) (-663.103) * (-672.890) (-668.870) (-659.516) [-665.032] -- 0:02:45 382000 -- (-675.354) (-670.397) [-665.383] (-661.362) * [-660.545] (-676.044) (-669.880) (-669.305) -- 0:02:45 383000 -- (-665.126) [-667.218] (-669.029) (-661.137) * (-671.253) (-673.519) (-666.721) [-661.012] -- 0:02:44 384000 -- (-677.495) (-662.579) [-667.522] (-672.275) * (-668.569) (-671.769) (-668.309) [-667.524] -- 0:02:43 385000 -- (-669.327) (-670.210) (-665.058) [-663.870] * (-671.162) [-667.627] (-672.757) (-663.372) -- 0:02:44 Average standard deviation of split frequencies: 0.008142 386000 -- (-667.781) [-660.110] (-659.836) (-667.703) * (-666.474) (-673.680) (-674.961) [-673.845] -- 0:02:43 387000 -- (-664.456) [-671.885] (-661.329) (-677.559) * (-660.242) [-662.600] (-666.287) (-670.266) -- 0:02:43 388000 -- (-667.546) [-665.229] (-667.599) (-670.489) * (-669.399) [-671.447] (-663.188) (-673.963) -- 0:02:42 389000 -- (-664.461) (-669.780) [-659.171] (-673.333) * (-661.064) (-663.646) (-671.277) [-666.497] -- 0:02:43 390000 -- (-666.504) (-659.354) (-673.444) [-659.657] * [-668.568] (-668.681) (-673.159) (-662.158) -- 0:02:42 Average standard deviation of split frequencies: 0.008044 391000 -- [-669.915] (-672.993) (-664.157) (-667.235) * (-665.325) (-661.153) (-668.584) [-666.141] -- 0:02:41 392000 -- (-676.379) (-675.552) [-662.165] (-669.206) * (-669.269) (-666.243) [-666.085] (-670.314) -- 0:02:42 393000 -- [-665.390] (-683.689) (-665.464) (-672.173) * (-668.246) (-676.181) [-669.887] (-664.322) -- 0:02:42 394000 -- [-667.193] (-672.793) (-663.869) (-665.705) * [-667.303] (-667.062) (-675.011) (-666.289) -- 0:02:41 395000 -- [-666.783] (-670.648) (-668.997) (-668.615) * (-667.238) (-662.354) (-668.817) [-661.568] -- 0:02:40 Average standard deviation of split frequencies: 0.008035 396000 -- (-668.391) [-668.544] (-661.005) (-668.539) * (-673.778) (-664.918) [-666.574] (-667.404) -- 0:02:41 397000 -- (-668.089) [-676.004] (-670.486) (-670.356) * (-673.332) (-665.973) (-662.565) [-662.046] -- 0:02:41 398000 -- (-668.644) (-666.874) (-676.069) [-661.047] * (-667.391) (-664.845) (-664.696) [-666.822] -- 0:02:40 399000 -- (-672.517) (-669.351) (-665.960) [-671.514] * (-672.967) (-664.318) (-666.107) [-667.369] -- 0:02:39 400000 -- (-664.071) (-671.209) (-668.452) [-663.156] * [-662.351] (-665.358) (-672.148) (-666.234) -- 0:02:40 Average standard deviation of split frequencies: 0.008001 401000 -- (-666.336) (-667.004) (-668.013) [-664.462] * [-669.841] (-672.476) (-673.610) (-671.396) -- 0:02:39 402000 -- (-665.046) [-663.913] (-667.406) (-667.430) * [-677.147] (-667.670) (-670.143) (-674.997) -- 0:02:39 403000 -- [-663.360] (-663.213) (-670.651) (-666.471) * (-663.176) (-663.435) [-668.600] (-676.901) -- 0:02:38 404000 -- (-666.471) (-667.227) (-666.862) [-669.766] * [-667.160] (-679.670) (-663.524) (-670.210) -- 0:02:39 405000 -- (-663.159) (-667.004) [-670.257] (-661.598) * (-669.940) (-662.413) (-672.537) [-666.361] -- 0:02:38 Average standard deviation of split frequencies: 0.008360 406000 -- (-667.898) (-666.563) (-671.454) [-667.573] * (-665.500) (-668.574) [-664.460] (-666.863) -- 0:02:38 407000 -- (-665.989) [-664.466] (-664.291) (-668.922) * [-660.659] (-662.783) (-661.872) (-666.463) -- 0:02:37 408000 -- (-663.168) [-669.834] (-685.465) (-670.463) * [-666.998] (-664.403) (-674.730) (-673.078) -- 0:02:38 409000 -- (-683.901) (-674.582) [-669.142] (-663.487) * [-661.909] (-670.955) (-666.967) (-670.608) -- 0:02:37 410000 -- (-668.209) (-676.647) (-668.373) [-666.676] * (-661.531) (-666.905) (-668.521) [-666.792] -- 0:02:36 Average standard deviation of split frequencies: 0.008691 411000 -- (-679.905) (-666.846) [-668.689] (-667.388) * (-667.991) (-665.818) (-661.535) [-675.376] -- 0:02:37 412000 -- (-676.544) (-671.838) (-666.609) [-664.951] * (-671.405) [-661.893] (-663.315) (-673.534) -- 0:02:36 413000 -- (-666.412) [-668.324] (-664.035) (-674.028) * (-667.847) [-662.653] (-670.817) (-669.767) -- 0:02:36 414000 -- (-664.129) (-667.352) [-670.324] (-669.392) * [-662.373] (-670.042) (-680.539) (-664.773) -- 0:02:35 415000 -- [-663.487] (-666.185) (-663.757) (-664.590) * (-669.275) (-671.191) (-667.535) [-660.140] -- 0:02:36 Average standard deviation of split frequencies: 0.008634 416000 -- (-667.807) (-673.882) (-666.892) [-659.039] * (-678.829) (-662.750) [-665.259] (-668.765) -- 0:02:35 417000 -- (-670.866) (-670.649) [-667.550] (-664.605) * (-676.858) (-663.857) (-671.349) [-663.233] -- 0:02:35 418000 -- (-672.737) (-674.987) (-667.249) [-660.643] * (-661.441) (-672.054) (-667.629) [-670.044] -- 0:02:34 419000 -- [-665.320] (-679.219) (-667.327) (-667.059) * [-664.590] (-667.540) (-670.273) (-666.874) -- 0:02:35 420000 -- [-663.612] (-679.437) (-668.676) (-668.303) * [-666.321] (-669.951) (-661.821) (-675.836) -- 0:02:34 Average standard deviation of split frequencies: 0.008685 421000 -- (-669.676) (-670.121) (-660.658) [-668.552] * (-662.026) [-672.887] (-663.252) (-668.735) -- 0:02:34 422000 -- (-669.558) (-662.968) (-678.635) [-664.191] * (-662.194) (-669.129) [-669.785] (-673.531) -- 0:02:33 423000 -- (-668.482) [-669.169] (-667.197) (-677.831) * [-668.497] (-682.870) (-665.951) (-662.414) -- 0:02:34 424000 -- (-665.326) [-668.793] (-667.020) (-671.800) * [-661.468] (-676.485) (-668.568) (-672.818) -- 0:02:33 425000 -- (-667.627) [-663.691] (-673.979) (-662.006) * (-663.888) (-663.855) (-670.799) [-662.411] -- 0:02:32 Average standard deviation of split frequencies: 0.008133 426000 -- (-680.790) (-672.232) (-672.017) [-663.675] * [-660.721] (-671.812) (-675.544) (-671.701) -- 0:02:33 427000 -- (-674.446) (-673.640) [-668.947] (-669.074) * [-662.381] (-661.587) (-676.359) (-669.986) -- 0:02:32 428000 -- (-680.652) [-670.462] (-670.181) (-666.025) * (-667.406) (-665.261) (-670.096) [-662.081] -- 0:02:32 429000 -- [-662.263] (-674.952) (-666.634) (-665.265) * (-662.503) (-671.156) (-668.090) [-664.523] -- 0:02:31 430000 -- (-669.844) [-670.067] (-660.693) (-670.238) * (-680.054) (-675.632) [-661.560] (-662.302) -- 0:02:32 Average standard deviation of split frequencies: 0.008483 431000 -- (-671.909) (-670.416) (-672.160) [-665.143] * [-669.377] (-677.948) (-673.482) (-660.101) -- 0:02:31 432000 -- (-669.593) [-665.478] (-658.521) (-672.933) * (-666.221) (-666.633) [-663.691] (-671.534) -- 0:02:31 433000 -- (-670.787) [-662.650] (-664.836) (-663.139) * (-666.641) [-667.735] (-672.765) (-670.645) -- 0:02:30 434000 -- (-666.717) (-669.156) (-663.923) [-664.390] * [-669.263] (-666.330) (-667.328) (-670.259) -- 0:02:31 435000 -- (-664.216) [-668.820] (-674.808) (-666.399) * (-667.998) (-668.897) [-667.633] (-675.140) -- 0:02:30 Average standard deviation of split frequencies: 0.008704 436000 -- [-673.298] (-668.122) (-677.128) (-664.855) * (-670.720) (-663.567) (-668.334) [-666.813] -- 0:02:30 437000 -- [-663.698] (-668.046) (-666.266) (-670.170) * (-668.066) (-661.063) [-666.222] (-669.290) -- 0:02:29 438000 -- (-662.511) (-666.676) (-669.924) [-668.809] * (-666.598) (-673.490) [-667.689] (-671.245) -- 0:02:30 439000 -- [-664.016] (-673.329) (-663.470) (-663.871) * (-667.067) (-662.984) [-669.171] (-675.094) -- 0:02:29 440000 -- [-664.524] (-673.531) (-667.123) (-670.490) * (-666.950) (-667.776) [-670.639] (-669.026) -- 0:02:28 Average standard deviation of split frequencies: 0.008665 441000 -- [-664.921] (-672.472) (-669.858) (-669.493) * [-673.560] (-661.567) (-661.411) (-663.721) -- 0:02:29 442000 -- (-665.691) (-669.263) (-674.032) [-663.256] * (-677.984) (-670.841) [-663.865] (-663.224) -- 0:02:28 443000 -- (-665.822) (-663.867) [-667.765] (-667.465) * (-666.774) (-678.425) [-666.669] (-672.130) -- 0:02:28 444000 -- (-665.110) (-665.098) [-665.927] (-668.504) * (-659.432) (-677.457) [-659.080] (-665.568) -- 0:02:27 445000 -- (-668.680) [-667.018] (-660.731) (-671.058) * (-661.502) [-669.078] (-660.760) (-675.577) -- 0:02:28 Average standard deviation of split frequencies: 0.008561 446000 -- [-668.140] (-672.365) (-669.388) (-667.618) * (-666.429) (-670.015) [-667.540] (-665.411) -- 0:02:27 447000 -- [-661.569] (-671.777) (-669.995) (-674.411) * (-663.652) (-672.914) [-667.291] (-672.496) -- 0:02:27 448000 -- (-666.833) (-668.350) (-668.116) [-669.692] * (-664.377) (-665.751) (-675.911) [-661.749] -- 0:02:26 449000 -- [-666.968] (-665.298) (-675.722) (-667.451) * (-663.831) (-664.146) [-660.849] (-673.520) -- 0:02:27 450000 -- (-662.939) [-666.391] (-665.560) (-668.958) * (-672.502) [-667.813] (-676.319) (-667.268) -- 0:02:26 Average standard deviation of split frequencies: 0.008002 451000 -- [-667.495] (-662.617) (-670.283) (-664.351) * (-665.459) [-673.458] (-670.933) (-668.828) -- 0:02:26 452000 -- (-671.657) (-674.904) [-670.394] (-667.776) * (-666.519) [-668.222] (-664.641) (-669.728) -- 0:02:25 453000 -- (-668.907) [-668.482] (-669.260) (-686.011) * (-673.609) (-662.266) [-664.708] (-667.182) -- 0:02:26 454000 -- (-665.721) (-667.780) (-671.762) [-668.200] * [-669.513] (-670.827) (-669.912) (-666.001) -- 0:02:25 455000 -- (-660.829) [-661.283] (-663.720) (-679.106) * (-668.423) (-677.963) [-666.408] (-669.170) -- 0:02:24 Average standard deviation of split frequencies: 0.007340 456000 -- (-669.943) (-672.781) [-666.697] (-661.106) * (-668.776) (-677.317) [-663.983] (-667.678) -- 0:02:25 457000 -- (-661.402) [-665.333] (-667.853) (-670.650) * [-668.197] (-670.436) (-679.068) (-671.749) -- 0:02:24 458000 -- [-668.213] (-665.340) (-666.379) (-663.992) * [-663.345] (-675.187) (-676.901) (-671.164) -- 0:02:24 459000 -- (-676.285) [-664.826] (-669.409) (-666.882) * (-668.538) (-677.355) (-665.308) [-659.234] -- 0:02:23 460000 -- (-664.129) (-668.761) (-683.465) [-663.016] * (-666.425) (-672.268) [-661.150] (-670.847) -- 0:02:24 Average standard deviation of split frequencies: 0.006920 461000 -- (-665.868) (-670.333) [-667.259] (-668.945) * (-671.222) (-665.414) (-669.302) [-665.999] -- 0:02:23 462000 -- [-667.909] (-667.869) (-663.780) (-663.012) * (-666.431) (-665.803) (-665.637) [-672.565] -- 0:02:23 463000 -- (-667.143) (-669.924) [-667.887] (-669.540) * (-665.186) [-667.056] (-668.645) (-671.346) -- 0:02:22 464000 -- (-671.026) (-668.055) [-665.833] (-669.273) * (-662.533) [-670.849] (-662.448) (-674.769) -- 0:02:23 465000 -- [-666.884] (-668.636) (-673.542) (-666.981) * (-670.917) (-669.802) (-671.587) [-663.308] -- 0:02:22 Average standard deviation of split frequencies: 0.007033 466000 -- (-665.684) (-663.254) (-662.930) [-666.956] * [-673.628] (-666.743) (-668.756) (-668.795) -- 0:02:22 467000 -- (-675.363) (-666.754) (-669.122) [-663.609] * (-663.755) [-668.863] (-667.866) (-667.218) -- 0:02:21 468000 -- (-661.927) (-670.516) [-662.808] (-670.725) * (-665.831) [-667.329] (-665.469) (-674.299) -- 0:02:22 469000 -- (-664.799) [-665.133] (-676.090) (-668.047) * (-673.609) [-664.491] (-664.990) (-676.161) -- 0:02:21 470000 -- [-663.694] (-676.720) (-669.424) (-669.731) * (-675.338) (-660.246) [-668.781] (-670.596) -- 0:02:20 Average standard deviation of split frequencies: 0.007412 471000 -- (-671.463) (-679.132) (-667.815) [-668.273] * [-672.886] (-668.169) (-667.741) (-668.255) -- 0:02:21 472000 -- (-671.380) (-665.161) [-662.235] (-667.431) * (-670.690) [-669.269] (-662.492) (-676.051) -- 0:02:20 473000 -- [-666.991] (-666.630) (-663.769) (-674.957) * (-666.118) (-667.362) (-667.593) [-661.968] -- 0:02:20 474000 -- [-662.189] (-671.540) (-665.955) (-666.399) * (-673.611) [-658.746] (-667.181) (-668.198) -- 0:02:19 475000 -- (-670.013) (-671.021) [-665.328] (-666.022) * (-672.517) (-665.814) (-669.384) [-668.525] -- 0:02:20 Average standard deviation of split frequencies: 0.007230 476000 -- (-665.542) [-662.344] (-664.146) (-682.216) * (-664.770) (-677.806) [-670.759] (-664.996) -- 0:02:19 477000 -- [-664.228] (-665.428) (-667.895) (-663.311) * [-658.991] (-665.943) (-668.582) (-664.618) -- 0:02:19 478000 -- (-662.504) (-670.803) [-662.913] (-661.035) * [-664.334] (-668.347) (-670.747) (-679.302) -- 0:02:18 479000 -- [-666.048] (-664.515) (-660.016) (-670.546) * (-677.886) [-667.855] (-672.511) (-667.860) -- 0:02:19 480000 -- (-674.567) (-674.465) [-666.233] (-671.592) * (-674.263) (-670.798) (-667.913) [-666.854] -- 0:02:18 Average standard deviation of split frequencies: 0.008207 481000 -- (-673.164) [-671.180] (-670.557) (-671.026) * (-671.687) (-668.356) [-661.480] (-669.907) -- 0:02:18 482000 -- (-665.927) (-662.370) [-664.258] (-673.261) * (-664.586) [-661.896] (-668.945) (-676.602) -- 0:02:17 483000 -- (-665.698) (-662.761) [-668.388] (-676.806) * [-669.427] (-674.993) (-669.248) (-666.734) -- 0:02:18 484000 -- (-667.922) (-669.897) [-665.288] (-673.136) * [-664.076] (-658.101) (-669.104) (-667.127) -- 0:02:17 485000 -- (-663.455) [-667.791] (-666.148) (-669.534) * (-672.554) [-671.048] (-677.576) (-667.351) -- 0:02:16 Average standard deviation of split frequencies: 0.008321 486000 -- (-667.651) [-663.486] (-678.242) (-659.588) * (-667.239) [-663.743] (-668.306) (-675.515) -- 0:02:17 487000 -- (-678.645) (-667.355) (-671.874) [-664.137] * (-666.058) (-672.737) [-664.119] (-681.491) -- 0:02:16 488000 -- (-670.399) (-670.425) (-668.126) [-665.297] * (-663.680) [-661.988] (-669.906) (-665.125) -- 0:02:16 489000 -- (-680.834) (-671.831) [-666.712] (-673.867) * (-670.061) (-668.659) [-661.879] (-667.126) -- 0:02:15 490000 -- (-672.753) [-658.465] (-665.561) (-668.170) * [-664.565] (-665.841) (-666.740) (-667.102) -- 0:02:16 Average standard deviation of split frequencies: 0.007686 491000 -- (-682.665) (-658.877) [-666.145] (-663.005) * (-665.819) [-660.141] (-672.035) (-668.496) -- 0:02:15 492000 -- (-666.343) (-660.006) [-665.992] (-669.064) * (-668.115) (-665.429) (-670.616) [-667.493] -- 0:02:15 493000 -- (-678.763) (-669.925) (-669.908) [-659.686] * (-666.694) (-663.607) [-662.312] (-662.043) -- 0:02:14 494000 -- (-664.571) (-662.904) [-670.686] (-667.153) * (-675.978) (-664.969) (-662.193) [-662.973] -- 0:02:15 495000 -- (-669.725) (-671.959) [-660.595] (-661.967) * (-672.711) (-667.419) [-665.017] (-665.243) -- 0:02:14 Average standard deviation of split frequencies: 0.007903 496000 -- (-666.108) (-676.297) [-669.091] (-674.250) * [-670.860] (-666.072) (-669.066) (-667.656) -- 0:02:14 497000 -- [-665.485] (-671.762) (-664.015) (-667.609) * (-664.014) (-666.476) (-663.985) [-670.109] -- 0:02:13 498000 -- [-667.583] (-663.977) (-663.257) (-669.382) * (-660.985) [-668.923] (-675.510) (-669.531) -- 0:02:14 499000 -- (-673.182) (-670.039) [-659.572] (-670.868) * (-660.993) (-669.445) (-673.300) [-665.696] -- 0:02:13 500000 -- (-671.575) (-663.888) [-663.647] (-667.774) * [-666.289] (-668.259) (-675.054) (-660.548) -- 0:02:13 Average standard deviation of split frequencies: 0.008127 501000 -- (-664.528) [-659.402] (-664.590) (-668.608) * (-668.233) [-672.498] (-668.277) (-663.403) -- 0:02:13 502000 -- (-661.653) (-665.396) [-661.532] (-671.974) * (-669.386) [-670.732] (-667.441) (-666.974) -- 0:02:12 503000 -- [-666.661] (-669.275) (-665.940) (-680.690) * (-668.724) (-675.222) (-666.611) [-665.977] -- 0:02:12 504000 -- (-666.563) (-672.387) (-665.477) [-665.049] * (-671.187) (-665.999) (-672.680) [-662.672] -- 0:02:11 505000 -- (-665.129) (-671.876) (-669.476) [-666.281] * [-667.826] (-677.685) (-671.495) (-671.764) -- 0:02:12 Average standard deviation of split frequencies: 0.008728 506000 -- (-672.364) (-672.398) (-678.194) [-664.301] * [-665.384] (-669.216) (-669.943) (-663.489) -- 0:02:11 507000 -- (-659.963) [-663.398] (-673.803) (-676.960) * (-660.486) (-661.174) [-665.875] (-670.657) -- 0:02:11 508000 -- (-675.777) [-671.655] (-676.738) (-664.788) * (-667.055) (-663.881) (-673.786) [-666.036] -- 0:02:10 509000 -- (-671.602) (-669.324) [-662.730] (-671.526) * (-662.978) [-661.349] (-664.578) (-673.386) -- 0:02:11 510000 -- (-661.924) [-664.227] (-667.148) (-668.892) * (-664.868) (-666.681) (-666.184) [-662.358] -- 0:02:10 Average standard deviation of split frequencies: 0.009134 511000 -- [-668.625] (-666.716) (-663.405) (-667.553) * (-676.424) (-671.517) (-671.750) [-665.045] -- 0:02:10 512000 -- (-677.406) (-664.380) [-663.169] (-664.530) * (-668.834) [-660.951] (-670.250) (-666.849) -- 0:02:10 513000 -- [-671.242] (-666.004) (-663.607) (-671.814) * (-668.007) (-662.300) (-661.768) [-662.815] -- 0:02:10 514000 -- (-663.233) [-663.787] (-679.598) (-670.922) * [-669.096] (-664.210) (-668.585) (-668.585) -- 0:02:09 515000 -- (-669.623) (-671.408) [-661.465] (-675.586) * [-668.223] (-663.345) (-665.654) (-669.355) -- 0:02:09 Average standard deviation of split frequencies: 0.009280 516000 -- (-666.109) [-662.891] (-665.541) (-666.083) * [-665.855] (-663.031) (-667.689) (-670.506) -- 0:02:09 517000 -- (-668.636) (-667.672) [-669.332] (-666.971) * (-670.199) (-670.654) [-664.130] (-666.435) -- 0:02:08 518000 -- (-670.671) (-671.057) (-668.788) [-660.945] * (-668.021) [-671.675] (-667.569) (-668.865) -- 0:02:08 519000 -- (-674.116) (-663.961) [-667.894] (-679.628) * [-668.521] (-673.479) (-669.876) (-671.239) -- 0:02:07 520000 -- [-658.682] (-663.627) (-665.942) (-662.784) * [-662.387] (-672.227) (-668.096) (-663.813) -- 0:02:08 Average standard deviation of split frequencies: 0.008911 521000 -- (-665.250) [-662.898] (-666.733) (-671.465) * (-665.089) (-669.891) [-664.557] (-669.133) -- 0:02:07 522000 -- [-671.455] (-664.952) (-678.780) (-674.602) * (-671.802) [-661.451] (-667.830) (-661.728) -- 0:02:07 523000 -- [-665.760] (-666.670) (-671.413) (-663.756) * (-668.517) (-678.881) (-668.284) [-665.579] -- 0:02:06 524000 -- [-662.685] (-676.547) (-671.057) (-668.631) * (-669.674) [-659.243] (-669.215) (-673.122) -- 0:02:07 525000 -- [-666.345] (-663.322) (-671.064) (-679.160) * (-669.273) (-672.464) (-676.280) [-664.933] -- 0:02:06 Average standard deviation of split frequencies: 0.009104 526000 -- (-666.315) [-663.752] (-663.947) (-669.392) * (-673.997) (-666.001) [-664.078] (-667.063) -- 0:02:06 527000 -- (-662.954) (-668.600) (-672.445) [-665.200] * [-669.146] (-664.217) (-674.927) (-664.846) -- 0:02:06 528000 -- [-657.284] (-663.881) (-664.675) (-667.807) * (-666.282) [-663.135] (-662.590) (-664.487) -- 0:02:06 529000 -- (-668.937) [-668.619] (-669.544) (-666.037) * [-667.204] (-664.313) (-666.825) (-665.238) -- 0:02:05 530000 -- [-669.558] (-672.780) (-674.212) (-672.662) * [-660.730] (-668.668) (-672.696) (-667.511) -- 0:02:05 Average standard deviation of split frequencies: 0.008930 531000 -- [-661.849] (-660.671) (-670.035) (-673.036) * [-659.875] (-666.631) (-666.145) (-675.481) -- 0:02:05 532000 -- (-664.509) (-665.587) [-668.307] (-671.118) * (-666.322) [-664.415] (-667.410) (-670.733) -- 0:02:04 533000 -- (-665.816) (-674.578) [-659.975] (-659.946) * [-665.922] (-671.466) (-671.661) (-666.211) -- 0:02:04 534000 -- (-665.318) (-660.460) [-660.261] (-667.860) * [-664.392] (-661.163) (-676.337) (-670.393) -- 0:02:03 535000 -- (-667.081) (-667.583) (-661.835) [-670.925] * (-662.600) [-670.639] (-667.569) (-665.934) -- 0:02:04 Average standard deviation of split frequencies: 0.009258 536000 -- [-662.277] (-675.217) (-672.531) (-663.038) * (-671.644) [-663.311] (-667.508) (-666.922) -- 0:02:03 537000 -- [-660.205] (-671.259) (-668.324) (-672.135) * (-673.176) (-664.176) (-673.137) [-667.711] -- 0:02:03 538000 -- (-670.956) (-665.586) (-672.119) [-658.360] * [-664.405] (-665.003) (-667.338) (-662.698) -- 0:02:02 539000 -- [-663.801] (-667.707) (-666.293) (-664.974) * (-667.517) (-670.611) [-665.842] (-669.113) -- 0:02:03 540000 -- [-671.294] (-669.151) (-664.559) (-662.083) * (-664.997) [-664.943] (-669.617) (-679.345) -- 0:02:02 Average standard deviation of split frequencies: 0.009460 541000 -- (-674.737) (-667.643) (-670.028) [-667.057] * (-662.736) (-664.611) [-665.525] (-670.838) -- 0:02:02 542000 -- (-674.441) (-672.953) [-658.922] (-664.315) * (-669.442) (-664.963) (-670.815) [-661.127] -- 0:02:02 543000 -- [-664.195] (-668.004) (-667.622) (-671.867) * (-667.409) (-664.188) (-671.360) [-661.224] -- 0:02:02 544000 -- (-674.641) [-661.813] (-667.769) (-663.057) * (-665.566) (-661.245) [-666.762] (-668.067) -- 0:02:01 545000 -- [-666.644] (-667.764) (-658.956) (-676.375) * (-665.263) (-666.338) (-670.508) [-659.431] -- 0:02:01 Average standard deviation of split frequencies: 0.009368 546000 -- (-664.639) [-666.218] (-672.213) (-667.996) * (-677.212) [-666.278] (-673.797) (-671.924) -- 0:02:01 547000 -- (-666.725) (-673.591) (-675.693) [-666.732] * [-672.027] (-673.445) (-667.591) (-669.838) -- 0:02:00 548000 -- (-677.760) (-664.298) (-673.194) [-669.055] * (-674.668) (-670.516) [-674.833] (-665.398) -- 0:02:00 549000 -- (-674.526) (-668.144) [-666.671] (-667.138) * (-664.091) [-664.366] (-663.332) (-683.653) -- 0:01:59 550000 -- (-661.662) (-667.738) [-668.009] (-667.948) * (-676.162) [-664.249] (-678.036) (-670.967) -- 0:02:00 Average standard deviation of split frequencies: 0.010183 551000 -- (-664.507) (-669.775) (-672.109) [-664.046] * (-661.148) (-670.560) [-661.829] (-669.688) -- 0:01:59 552000 -- (-667.911) (-667.742) (-666.450) [-663.891] * (-667.278) (-683.159) (-665.942) [-671.856] -- 0:01:59 553000 -- (-669.161) [-665.395] (-667.866) (-662.880) * [-661.619] (-668.728) (-666.576) (-673.927) -- 0:01:59 554000 -- (-666.127) (-664.939) (-664.688) [-662.124] * (-673.455) (-665.716) (-668.529) [-662.513] -- 0:01:59 555000 -- (-670.172) (-669.669) [-662.413] (-664.088) * (-665.769) (-666.267) [-659.428] (-674.140) -- 0:01:58 Average standard deviation of split frequencies: 0.009728 556000 -- (-664.960) (-673.193) (-669.686) [-666.799] * [-669.270] (-660.208) (-677.472) (-671.090) -- 0:01:58 557000 -- [-672.019] (-667.679) (-662.586) (-670.817) * (-668.809) (-671.802) [-662.120] (-665.931) -- 0:01:58 558000 -- (-671.309) [-665.164] (-669.705) (-674.524) * [-670.968] (-663.605) (-669.691) (-666.330) -- 0:01:58 559000 -- (-664.388) (-663.613) (-673.175) [-661.521] * (-663.880) [-665.938] (-668.328) (-668.711) -- 0:01:57 560000 -- [-663.499] (-671.689) (-670.128) (-666.113) * (-671.551) (-680.728) [-665.699] (-664.149) -- 0:01:57 Average standard deviation of split frequencies: 0.009426 561000 -- (-672.997) [-671.882] (-677.682) (-665.184) * (-666.879) [-669.867] (-663.172) (-667.580) -- 0:01:57 562000 -- (-668.026) [-664.252] (-679.135) (-666.783) * (-670.454) (-669.956) [-670.850] (-664.682) -- 0:01:56 563000 -- [-665.863] (-676.734) (-671.001) (-664.306) * (-668.992) [-665.188] (-674.479) (-659.110) -- 0:01:56 564000 -- (-664.461) (-674.979) [-657.683] (-669.604) * [-667.607] (-673.907) (-668.972) (-678.090) -- 0:01:55 565000 -- (-671.213) (-668.783) (-667.744) [-665.607] * (-665.690) (-669.194) (-666.145) [-670.394] -- 0:01:56 Average standard deviation of split frequencies: 0.009249 566000 -- (-668.949) (-667.935) [-665.230] (-667.928) * (-675.626) [-663.422] (-670.249) (-672.967) -- 0:01:55 567000 -- (-664.885) [-659.616] (-665.236) (-665.670) * (-668.025) [-666.664] (-667.743) (-671.170) -- 0:01:55 568000 -- (-663.228) [-665.855] (-673.568) (-669.012) * (-671.813) (-670.555) (-662.753) [-666.618] -- 0:01:55 569000 -- (-659.242) (-666.129) [-665.182] (-675.291) * (-671.525) (-661.556) [-661.191] (-663.836) -- 0:01:55 570000 -- [-664.156] (-673.943) (-664.406) (-671.422) * [-658.445] (-676.496) (-670.306) (-671.998) -- 0:01:54 Average standard deviation of split frequencies: 0.009169 571000 -- (-667.853) (-671.417) (-663.897) [-665.886] * [-672.385] (-664.304) (-680.591) (-664.894) -- 0:01:54 572000 -- (-678.804) (-662.417) [-663.309] (-671.933) * (-676.705) [-662.317] (-664.485) (-671.072) -- 0:01:54 573000 -- (-672.888) [-661.478] (-660.269) (-665.869) * (-661.437) [-664.452] (-665.957) (-661.458) -- 0:01:54 574000 -- [-664.581] (-678.557) (-668.762) (-667.992) * (-666.035) (-668.048) [-660.339] (-664.132) -- 0:01:53 575000 -- (-664.807) (-668.913) [-660.557] (-662.517) * (-663.125) (-674.009) [-672.658] (-669.510) -- 0:01:53 Average standard deviation of split frequencies: 0.008880 576000 -- (-668.948) (-667.549) (-667.738) [-662.546] * (-662.473) (-661.176) (-667.311) [-659.029] -- 0:01:53 577000 -- (-664.567) (-670.253) [-668.228] (-666.584) * (-667.166) (-666.760) [-671.475] (-667.700) -- 0:01:52 578000 -- [-666.182] (-669.036) (-665.433) (-676.798) * [-665.640] (-663.935) (-668.877) (-674.849) -- 0:01:52 579000 -- (-663.942) [-661.934] (-664.217) (-673.771) * (-660.930) [-673.175] (-665.542) (-678.362) -- 0:01:51 580000 -- (-661.870) (-667.762) [-668.017] (-673.305) * (-661.805) (-666.798) (-662.406) [-665.197] -- 0:01:52 Average standard deviation of split frequencies: 0.008524 581000 -- (-664.184) (-666.947) [-668.237] (-668.438) * (-665.570) (-672.942) (-665.988) [-662.514] -- 0:01:51 582000 -- (-666.711) [-669.094] (-667.473) (-666.660) * (-673.290) (-670.572) (-670.225) [-665.390] -- 0:01:51 583000 -- (-663.850) (-666.379) (-663.861) [-663.691] * (-672.946) [-666.270] (-668.842) (-672.754) -- 0:01:51 584000 -- (-670.764) [-663.054] (-662.692) (-663.153) * (-667.370) [-675.311] (-672.532) (-665.645) -- 0:01:51 585000 -- (-667.344) (-664.983) [-662.532] (-664.693) * (-669.964) (-679.634) [-664.497] (-666.430) -- 0:01:50 Average standard deviation of split frequencies: 0.008608 586000 -- (-671.641) (-664.653) (-667.037) [-664.275] * (-667.166) (-674.052) (-669.046) [-663.346] -- 0:01:50 587000 -- (-668.057) (-663.647) (-667.051) [-667.584] * (-662.499) [-663.463] (-666.283) (-668.663) -- 0:01:50 588000 -- [-672.431] (-673.265) (-664.174) (-667.998) * (-662.687) (-662.392) [-664.248] (-674.245) -- 0:01:50 589000 -- (-665.182) (-668.851) (-665.821) [-669.272] * [-660.540] (-668.857) (-672.811) (-665.421) -- 0:01:49 590000 -- (-664.544) (-672.308) [-669.667] (-669.516) * [-666.665] (-665.958) (-665.386) (-675.307) -- 0:01:49 Average standard deviation of split frequencies: 0.008061 591000 -- (-661.732) (-661.027) (-671.559) [-671.491] * (-675.561) (-674.407) [-665.745] (-668.733) -- 0:01:49 592000 -- (-664.785) (-666.780) [-666.880] (-670.210) * (-663.795) (-669.969) (-669.364) [-673.338] -- 0:01:48 593000 -- (-676.248) (-675.463) [-667.457] (-664.017) * [-664.901] (-669.885) (-669.701) (-669.173) -- 0:01:48 594000 -- (-670.545) [-664.914] (-664.741) (-664.415) * (-670.341) [-663.596] (-676.291) (-671.677) -- 0:01:48 595000 -- (-665.353) (-667.317) (-672.755) [-662.479] * [-680.149] (-673.340) (-668.370) (-664.196) -- 0:01:48 Average standard deviation of split frequencies: 0.008576 596000 -- (-671.832) [-659.921] (-668.883) (-670.601) * (-664.586) (-664.943) (-668.158) [-670.342] -- 0:01:47 597000 -- (-665.575) [-663.010] (-665.983) (-664.862) * [-662.288] (-661.661) (-678.722) (-667.735) -- 0:01:48 598000 -- (-662.466) (-670.295) [-668.295] (-669.709) * (-674.947) [-662.662] (-673.014) (-677.181) -- 0:01:47 599000 -- [-664.823] (-666.806) (-671.124) (-665.258) * (-670.342) (-670.052) (-671.971) [-670.406] -- 0:01:47 600000 -- (-663.519) (-671.103) [-666.183] (-668.379) * (-675.302) [-662.202] (-661.387) (-678.580) -- 0:01:46 Average standard deviation of split frequencies: 0.008137 601000 -- [-666.303] (-675.088) (-662.251) (-672.146) * [-667.257] (-671.137) (-671.693) (-669.031) -- 0:01:46 602000 -- [-667.138] (-674.365) (-673.901) (-665.624) * (-668.487) (-671.028) [-673.121] (-669.826) -- 0:01:46 603000 -- [-673.268] (-660.875) (-666.793) (-664.430) * (-665.976) [-667.478] (-671.036) (-678.230) -- 0:01:45 604000 -- (-673.330) (-668.258) [-666.977] (-672.010) * (-664.560) (-666.274) [-662.151] (-663.281) -- 0:01:46 605000 -- (-669.235) [-665.866] (-663.382) (-673.851) * (-659.057) (-672.698) [-674.314] (-682.786) -- 0:01:45 Average standard deviation of split frequencies: 0.007973 606000 -- (-688.746) (-667.044) (-669.549) [-669.186] * (-663.907) (-663.998) [-665.704] (-661.709) -- 0:01:45 607000 -- (-668.589) [-662.038] (-670.875) (-671.153) * (-676.843) (-667.138) [-657.617] (-662.456) -- 0:01:44 608000 -- (-665.072) [-658.857] (-674.427) (-666.994) * (-668.252) (-664.302) (-668.094) [-664.496] -- 0:01:45 609000 -- (-666.366) (-664.349) (-667.714) [-666.596] * [-667.153] (-664.914) (-661.349) (-677.018) -- 0:01:44 610000 -- (-668.621) (-663.985) (-664.120) [-672.771] * (-659.038) (-673.666) (-671.215) [-663.746] -- 0:01:44 Average standard deviation of split frequencies: 0.008207 611000 -- (-665.976) (-671.370) (-665.191) [-664.800] * (-661.054) (-671.896) [-666.583] (-673.285) -- 0:01:43 612000 -- (-662.901) (-670.143) (-672.006) [-665.212] * [-664.782] (-665.570) (-673.606) (-675.250) -- 0:01:43 613000 -- (-670.252) (-664.679) (-677.036) [-667.390] * (-667.399) (-668.012) (-661.457) [-669.479] -- 0:01:43 614000 -- (-665.631) (-668.937) [-662.942] (-669.003) * (-661.932) (-676.828) (-665.618) [-668.856] -- 0:01:43 615000 -- (-672.327) (-662.096) (-678.861) [-668.949] * (-665.825) (-672.051) (-667.068) [-667.471] -- 0:01:43 Average standard deviation of split frequencies: 0.008378 616000 -- (-666.331) (-678.235) (-665.702) [-663.861] * (-665.887) (-665.763) [-658.916] (-673.152) -- 0:01:42 617000 -- (-680.053) (-671.683) (-666.407) [-671.427] * [-660.634] (-676.099) (-669.393) (-663.399) -- 0:01:42 618000 -- [-665.435] (-666.154) (-675.330) (-664.308) * (-663.941) (-669.886) [-665.243] (-675.243) -- 0:01:41 619000 -- (-667.356) (-670.996) (-671.006) [-662.356] * [-666.096] (-665.876) (-666.315) (-665.886) -- 0:01:42 620000 -- (-675.274) (-664.471) (-677.962) [-663.423] * [-665.593] (-678.140) (-664.148) (-672.718) -- 0:01:41 Average standard deviation of split frequencies: 0.008874 621000 -- (-675.658) [-668.817] (-666.813) (-675.896) * [-667.630] (-679.045) (-662.859) (-664.824) -- 0:01:41 622000 -- (-671.923) [-666.442] (-667.433) (-666.002) * [-666.120] (-682.997) (-670.111) (-668.390) -- 0:01:40 623000 -- (-661.825) (-660.283) [-670.027] (-667.448) * (-669.226) (-677.673) (-667.172) [-661.141] -- 0:01:41 624000 -- [-666.430] (-666.374) (-659.087) (-666.897) * (-659.897) (-676.360) (-677.664) [-668.215] -- 0:01:40 625000 -- (-667.400) (-666.675) (-676.141) [-660.771] * (-665.042) (-673.434) [-668.173] (-671.904) -- 0:01:40 Average standard deviation of split frequencies: 0.009116 626000 -- [-666.165] (-672.702) (-666.654) (-665.667) * (-676.578) [-665.993] (-661.919) (-673.689) -- 0:01:40 627000 -- [-659.454] (-667.476) (-663.643) (-672.958) * [-666.141] (-680.011) (-666.197) (-667.322) -- 0:01:39 628000 -- (-664.998) (-675.551) [-670.792] (-674.065) * (-662.103) (-667.810) [-668.520] (-661.777) -- 0:01:39 629000 -- (-674.166) (-666.005) [-661.940] (-669.591) * (-668.733) [-662.329] (-670.815) (-659.691) -- 0:01:39 630000 -- (-667.487) (-675.103) [-664.615] (-667.493) * [-666.289] (-673.175) (-678.278) (-661.592) -- 0:01:39 Average standard deviation of split frequencies: 0.009048 631000 -- (-667.958) (-663.493) (-665.697) [-664.215] * (-663.618) [-664.229] (-677.564) (-672.346) -- 0:01:38 632000 -- (-665.460) [-665.509] (-662.693) (-671.863) * [-667.383] (-663.368) (-676.613) (-666.237) -- 0:01:38 633000 -- (-661.681) (-670.427) [-668.872] (-669.087) * (-666.158) (-658.654) (-671.124) [-668.966] -- 0:01:37 634000 -- (-662.057) (-672.356) [-660.095] (-665.145) * [-665.488] (-668.306) (-667.610) (-671.779) -- 0:01:38 635000 -- [-666.379] (-666.256) (-665.927) (-664.333) * (-679.699) (-666.047) [-665.055] (-666.749) -- 0:01:37 Average standard deviation of split frequencies: 0.009050 636000 -- (-666.986) (-671.067) (-680.169) [-664.667] * [-664.092] (-674.518) (-675.074) (-660.438) -- 0:01:37 637000 -- (-658.754) (-671.809) [-669.656] (-663.323) * (-664.676) (-671.358) (-664.254) [-665.872] -- 0:01:37 638000 -- (-672.196) (-669.123) (-668.935) [-664.056] * (-667.870) (-668.002) [-664.274] (-666.335) -- 0:01:37 639000 -- (-666.700) [-668.936] (-666.234) (-660.034) * [-666.708] (-667.953) (-660.588) (-668.783) -- 0:01:36 640000 -- (-666.096) (-677.442) (-669.906) [-665.931] * (-665.615) [-659.168] (-663.258) (-664.043) -- 0:01:36 Average standard deviation of split frequencies: 0.009087 641000 -- [-663.119] (-672.008) (-672.014) (-666.567) * (-667.446) (-665.662) (-669.416) [-660.397] -- 0:01:36 642000 -- (-671.226) (-671.771) [-670.297] (-669.031) * [-668.085] (-671.662) (-668.261) (-664.376) -- 0:01:35 643000 -- (-672.144) [-661.675] (-667.098) (-666.255) * (-670.841) [-664.483] (-664.502) (-664.988) -- 0:01:35 644000 -- (-667.587) (-668.848) (-673.406) [-667.807] * (-671.921) [-663.631] (-677.863) (-665.390) -- 0:01:35 645000 -- (-669.465) (-668.162) [-663.668] (-677.778) * (-663.220) (-670.543) (-665.656) [-663.361] -- 0:01:35 Average standard deviation of split frequencies: 0.008720 646000 -- (-671.186) (-669.462) [-672.611] (-675.984) * (-667.984) (-664.108) [-669.549] (-662.619) -- 0:01:34 647000 -- (-660.759) (-671.632) [-667.324] (-671.317) * (-672.027) (-665.472) (-666.573) [-664.257] -- 0:01:34 648000 -- (-669.911) [-670.783] (-668.891) (-667.239) * [-664.332] (-669.679) (-674.694) (-668.266) -- 0:01:33 649000 -- (-672.380) [-666.359] (-666.114) (-673.444) * (-666.731) (-667.775) [-661.173] (-671.646) -- 0:01:34 650000 -- (-661.422) [-663.022] (-666.847) (-665.251) * (-677.599) (-664.504) (-667.300) [-662.813] -- 0:01:33 Average standard deviation of split frequencies: 0.008763 651000 -- (-665.692) (-671.738) [-661.181] (-669.329) * (-678.410) (-666.632) (-668.765) [-666.139] -- 0:01:33 652000 -- (-662.693) [-665.483] (-668.678) (-670.270) * (-669.417) (-669.766) (-662.661) [-659.730] -- 0:01:32 653000 -- (-672.158) [-668.533] (-667.306) (-660.525) * (-671.885) (-667.558) (-667.901) [-665.092] -- 0:01:32 654000 -- (-669.046) (-666.483) [-665.680] (-667.892) * [-665.269] (-662.862) (-685.157) (-668.845) -- 0:01:32 655000 -- (-667.556) [-670.901] (-664.278) (-672.109) * (-683.048) [-660.016] (-665.598) (-674.302) -- 0:01:32 Average standard deviation of split frequencies: 0.008760 656000 -- (-668.165) (-670.265) (-669.497) [-660.005] * (-670.882) (-670.690) (-668.378) [-660.340] -- 0:01:32 657000 -- (-660.703) (-668.369) (-669.139) [-668.481] * (-663.804) [-661.952] (-663.399) (-662.964) -- 0:01:31 658000 -- (-670.393) [-676.869] (-673.160) (-667.697) * (-672.963) [-661.392] (-670.992) (-667.499) -- 0:01:31 659000 -- (-670.636) [-665.049] (-670.273) (-667.431) * (-675.419) [-668.492] (-670.962) (-667.353) -- 0:01:31 660000 -- [-661.614] (-668.508) (-664.594) (-671.179) * (-667.301) (-666.196) [-664.614] (-662.785) -- 0:01:31 Average standard deviation of split frequencies: 0.008705 661000 -- [-660.337] (-671.857) (-668.898) (-666.892) * (-666.383) (-669.156) [-659.598] (-662.169) -- 0:01:30 662000 -- (-661.647) [-666.857] (-668.565) (-667.571) * (-666.091) (-670.661) (-670.366) [-666.507] -- 0:01:30 663000 -- (-669.966) [-667.382] (-671.250) (-662.817) * (-668.044) (-671.244) (-673.752) [-669.535] -- 0:01:29 664000 -- (-668.925) (-672.045) [-668.892] (-668.880) * (-673.185) (-667.051) (-668.037) [-659.127] -- 0:01:30 665000 -- (-661.816) (-660.485) [-665.831] (-674.033) * [-663.446] (-673.398) (-672.898) (-669.666) -- 0:01:29 Average standard deviation of split frequencies: 0.008777 666000 -- (-659.896) [-663.537] (-661.019) (-671.404) * (-665.528) [-669.342] (-669.910) (-665.177) -- 0:01:29 667000 -- (-668.423) [-668.357] (-669.802) (-667.283) * [-672.821] (-666.870) (-662.901) (-667.627) -- 0:01:29 668000 -- [-668.692] (-671.172) (-671.427) (-670.484) * (-680.364) (-667.644) (-667.164) [-668.359] -- 0:01:28 669000 -- (-670.531) (-671.712) [-669.947] (-668.397) * (-680.582) (-671.460) [-661.858] (-670.584) -- 0:01:28 670000 -- (-672.705) [-662.030] (-674.683) (-671.818) * (-666.060) (-663.560) (-675.695) [-664.270] -- 0:01:28 Average standard deviation of split frequencies: 0.009173 671000 -- (-673.743) [-661.226] (-673.233) (-671.188) * [-665.422] (-672.615) (-670.523) (-667.789) -- 0:01:28 672000 -- (-672.692) [-661.767] (-660.417) (-673.304) * (-665.153) (-669.041) (-668.527) [-667.371] -- 0:01:27 673000 -- (-671.761) (-674.933) (-670.797) [-671.339] * [-661.527] (-675.614) (-670.642) (-669.453) -- 0:01:27 674000 -- (-669.470) [-667.781] (-663.979) (-670.984) * [-666.971] (-669.399) (-668.426) (-664.462) -- 0:01:27 675000 -- (-664.346) [-667.019] (-659.866) (-665.024) * (-667.943) (-671.713) (-664.762) [-664.970] -- 0:01:27 Average standard deviation of split frequencies: 0.008961 676000 -- [-664.645] (-677.307) (-664.010) (-665.563) * (-667.456) (-668.833) [-665.660] (-660.884) -- 0:01:26 677000 -- (-666.088) (-658.491) [-663.908] (-669.716) * [-669.476] (-662.490) (-671.190) (-666.837) -- 0:01:26 678000 -- (-661.271) (-662.577) (-669.309) [-665.334] * (-669.846) (-670.804) [-670.513] (-665.087) -- 0:01:26 679000 -- (-667.991) (-677.663) [-665.014] (-663.317) * (-672.717) [-662.944] (-667.781) (-665.750) -- 0:01:26 680000 -- (-660.378) (-671.691) [-668.616] (-665.970) * [-662.713] (-669.736) (-665.414) (-665.052) -- 0:01:25 Average standard deviation of split frequencies: 0.008934 681000 -- (-662.889) (-666.225) (-672.044) [-664.746] * (-667.762) (-665.567) (-672.917) [-664.930] -- 0:01:25 682000 -- [-667.371] (-665.136) (-661.735) (-664.511) * [-663.356] (-667.899) (-676.261) (-665.129) -- 0:01:25 683000 -- (-666.033) (-676.602) (-665.585) [-663.641] * [-666.745] (-661.458) (-660.631) (-675.421) -- 0:01:24 684000 -- [-661.654] (-672.745) (-667.430) (-662.868) * [-664.056] (-664.740) (-674.776) (-666.410) -- 0:01:24 685000 -- (-662.477) (-673.271) [-664.891] (-668.669) * (-661.157) (-664.834) [-668.987] (-674.164) -- 0:01:24 Average standard deviation of split frequencies: 0.008968 686000 -- (-665.737) [-665.180] (-669.181) (-673.497) * [-663.843] (-665.666) (-662.053) (-669.070) -- 0:01:24 687000 -- [-663.561] (-671.367) (-666.323) (-663.156) * (-670.765) (-670.256) [-670.760] (-669.705) -- 0:01:23 688000 -- (-667.021) [-665.170] (-671.256) (-673.842) * (-668.672) (-670.962) (-664.537) [-664.109] -- 0:01:23 689000 -- [-669.401] (-666.569) (-661.206) (-665.317) * [-668.601] (-667.868) (-665.500) (-670.434) -- 0:01:23 690000 -- (-666.975) [-670.727] (-672.120) (-668.578) * (-673.633) (-677.756) [-657.531] (-666.518) -- 0:01:23 Average standard deviation of split frequencies: 0.009009 691000 -- (-664.305) [-670.096] (-671.357) (-667.545) * [-662.676] (-672.024) (-665.765) (-671.138) -- 0:01:22 692000 -- (-668.069) (-666.925) (-665.445) [-674.098] * [-664.200] (-668.195) (-669.209) (-674.373) -- 0:01:22 693000 -- [-671.981] (-665.001) (-673.256) (-666.552) * [-667.659] (-664.817) (-667.260) (-682.457) -- 0:01:22 694000 -- (-666.428) (-669.116) (-665.151) [-667.611] * (-665.269) [-662.635] (-664.968) (-672.179) -- 0:01:22 695000 -- (-666.329) (-669.629) (-663.187) [-663.233] * (-667.046) [-673.031] (-665.910) (-676.253) -- 0:01:21 Average standard deviation of split frequencies: 0.009177 696000 -- (-668.024) (-662.130) (-672.168) [-662.285] * [-674.480] (-677.603) (-669.934) (-669.737) -- 0:01:21 697000 -- (-661.807) (-680.783) (-664.483) [-672.875] * (-667.005) [-662.081] (-668.969) (-663.778) -- 0:01:21 698000 -- [-666.407] (-669.132) (-663.881) (-673.113) * (-663.373) (-663.381) (-674.823) [-661.607] -- 0:01:20 699000 -- (-671.136) (-680.023) [-668.121] (-673.229) * (-671.465) (-662.666) (-666.085) [-660.853] -- 0:01:20 700000 -- [-664.218] (-669.989) (-670.684) (-665.318) * (-664.269) (-676.666) (-667.292) [-668.090] -- 0:01:20 Average standard deviation of split frequencies: 0.008982 701000 -- (-667.509) [-664.482] (-670.229) (-666.249) * [-659.340] (-671.557) (-673.297) (-664.525) -- 0:01:20 702000 -- [-664.794] (-672.974) (-667.586) (-681.613) * (-666.531) (-670.435) [-665.801] (-662.223) -- 0:01:19 703000 -- (-663.203) [-667.566] (-665.474) (-672.382) * (-669.072) (-676.521) [-666.935] (-666.637) -- 0:01:19 704000 -- (-668.015) (-667.212) [-664.462] (-671.333) * (-664.216) [-664.972] (-672.245) (-674.205) -- 0:01:19 705000 -- (-673.479) (-663.450) (-665.645) [-672.342] * (-671.105) (-667.835) [-662.896] (-664.520) -- 0:01:19 Average standard deviation of split frequencies: 0.008914 706000 -- (-665.276) (-680.760) (-667.539) [-672.278] * (-668.816) (-669.599) [-666.990] (-670.523) -- 0:01:18 707000 -- [-664.805] (-681.022) (-661.947) (-663.503) * (-672.596) [-666.163] (-669.745) (-670.525) -- 0:01:18 708000 -- (-665.506) (-666.512) (-666.823) [-667.163] * (-662.085) (-660.571) [-668.573] (-668.739) -- 0:01:18 709000 -- [-667.170] (-671.870) (-670.288) (-663.229) * [-668.587] (-670.065) (-669.183) (-670.121) -- 0:01:17 710000 -- (-665.885) (-673.878) (-673.050) [-670.511] * (-664.124) (-666.465) [-671.412] (-671.270) -- 0:01:17 Average standard deviation of split frequencies: 0.009088 711000 -- [-668.846] (-667.656) (-669.441) (-665.059) * (-664.245) [-666.043] (-668.287) (-667.428) -- 0:01:17 712000 -- (-660.902) (-674.346) (-672.112) [-667.867] * (-666.839) [-666.576] (-665.661) (-667.619) -- 0:01:17 713000 -- (-665.529) [-661.387] (-669.062) (-675.584) * (-661.869) (-670.371) [-662.531] (-675.181) -- 0:01:16 714000 -- (-663.752) (-663.056) [-665.413] (-675.224) * [-678.570] (-673.756) (-672.242) (-668.144) -- 0:01:16 715000 -- (-669.053) [-664.633] (-663.539) (-666.467) * (-662.805) (-678.067) [-663.581] (-669.876) -- 0:01:16 Average standard deviation of split frequencies: 0.008524 716000 -- (-671.690) [-670.099] (-673.422) (-668.169) * (-665.040) (-668.776) [-665.218] (-676.029) -- 0:01:16 717000 -- (-665.426) (-677.368) (-666.942) [-671.455] * (-662.666) (-665.772) (-670.831) [-671.961] -- 0:01:15 718000 -- (-678.334) (-667.422) (-670.515) [-665.059] * (-663.812) (-676.164) (-671.060) [-664.956] -- 0:01:15 719000 -- (-676.297) [-663.119] (-669.450) (-670.214) * [-672.759] (-667.513) (-669.891) (-662.173) -- 0:01:15 720000 -- (-660.490) (-672.643) [-662.454] (-669.300) * [-659.462] (-665.060) (-674.576) (-673.158) -- 0:01:15 Average standard deviation of split frequencies: 0.008469 721000 -- [-664.822] (-676.829) (-669.082) (-660.651) * (-686.610) [-660.340] (-672.058) (-678.216) -- 0:01:14 722000 -- [-664.174] (-666.461) (-667.832) (-664.450) * [-661.739] (-663.296) (-676.644) (-668.395) -- 0:01:14 723000 -- (-664.134) (-671.340) [-667.728] (-665.763) * [-668.684] (-666.153) (-673.745) (-662.698) -- 0:01:14 724000 -- (-670.340) (-663.639) (-658.732) [-666.165] * (-671.560) [-662.945] (-665.768) (-665.783) -- 0:01:13 725000 -- [-663.729] (-664.280) (-662.684) (-676.364) * (-667.580) (-669.580) (-672.001) [-667.874] -- 0:01:13 Average standard deviation of split frequencies: 0.008920 726000 -- (-667.609) (-662.770) [-667.850] (-666.577) * (-677.210) (-677.976) (-662.618) [-665.491] -- 0:01:13 727000 -- (-671.517) (-666.079) [-663.654] (-672.081) * (-666.649) (-663.424) (-667.005) [-662.031] -- 0:01:13 728000 -- (-669.791) (-664.272) [-667.968] (-666.506) * (-662.773) [-662.433] (-666.074) (-669.634) -- 0:01:12 729000 -- (-664.147) (-675.003) [-669.432] (-662.568) * [-663.267] (-674.385) (-662.870) (-677.156) -- 0:01:12 730000 -- (-673.165) [-665.216] (-660.785) (-675.185) * (-667.454) [-673.904] (-668.278) (-677.970) -- 0:01:12 Average standard deviation of split frequencies: 0.008761 731000 -- (-667.833) [-662.141] (-673.547) (-664.812) * (-668.351) (-674.423) [-665.357] (-674.304) -- 0:01:12 732000 -- (-664.964) (-667.801) [-668.688] (-668.736) * (-665.383) (-672.509) [-665.716] (-660.024) -- 0:01:11 733000 -- (-660.728) (-672.300) (-670.133) [-660.677] * (-664.807) [-666.635] (-669.509) (-671.292) -- 0:01:11 734000 -- (-667.905) (-663.672) (-665.331) [-666.024] * (-673.002) (-664.744) (-664.290) [-660.172] -- 0:01:11 735000 -- [-660.727] (-677.606) (-666.768) (-667.965) * [-667.940] (-668.694) (-676.905) (-676.876) -- 0:01:11 Average standard deviation of split frequencies: 0.008428 736000 -- [-664.118] (-668.050) (-663.300) (-667.705) * (-674.065) [-667.902] (-674.395) (-679.032) -- 0:01:10 737000 -- (-673.211) (-672.519) [-661.735] (-663.101) * (-661.755) (-665.979) (-689.290) [-664.250] -- 0:01:10 738000 -- (-667.019) (-657.605) [-661.034] (-671.430) * [-664.563] (-664.846) (-676.092) (-672.716) -- 0:01:10 739000 -- (-664.074) [-665.325] (-661.418) (-677.054) * (-662.487) [-669.011] (-669.475) (-665.464) -- 0:01:09 740000 -- (-671.932) [-671.456] (-669.788) (-661.643) * (-659.328) (-675.379) [-667.911] (-668.508) -- 0:01:09 Average standard deviation of split frequencies: 0.008073 741000 -- (-672.935) (-666.985) (-660.933) [-667.980] * (-666.085) (-666.508) (-668.294) [-664.358] -- 0:01:09 742000 -- (-670.109) [-671.264] (-674.264) (-667.104) * (-663.211) (-668.952) (-671.034) [-661.917] -- 0:01:09 743000 -- (-682.243) (-663.253) (-670.489) [-666.121] * (-669.199) [-673.101] (-678.368) (-665.420) -- 0:01:08 744000 -- (-666.398) (-665.706) [-679.297] (-665.001) * (-665.010) (-662.796) (-675.423) [-661.516] -- 0:01:08 745000 -- (-668.017) (-672.995) [-667.998] (-669.512) * (-662.889) (-662.117) [-665.299] (-665.092) -- 0:01:08 Average standard deviation of split frequencies: 0.008315 746000 -- (-674.906) (-659.172) (-680.710) [-670.815] * (-668.125) [-663.802] (-672.433) (-666.011) -- 0:01:08 747000 -- (-665.289) (-671.159) (-672.035) [-676.916] * (-678.116) [-664.118] (-664.602) (-675.764) -- 0:01:07 748000 -- (-669.833) (-673.759) (-660.624) [-663.629] * (-668.916) (-664.723) (-671.427) [-663.671] -- 0:01:07 749000 -- [-662.127] (-674.310) (-671.671) (-665.458) * (-678.147) (-668.270) [-675.875] (-667.040) -- 0:01:07 750000 -- (-667.569) (-664.084) [-670.728] (-669.592) * [-671.999] (-664.566) (-671.302) (-669.079) -- 0:01:07 Average standard deviation of split frequencies: 0.008296 751000 -- (-671.878) (-680.683) (-665.166) [-671.554] * (-665.981) [-665.698] (-665.229) (-664.146) -- 0:01:06 752000 -- (-678.220) (-667.167) (-667.594) [-663.143] * (-667.290) [-669.768] (-664.707) (-666.350) -- 0:01:06 753000 -- (-665.433) [-671.763] (-672.371) (-673.347) * [-667.452] (-670.576) (-663.444) (-668.102) -- 0:01:06 754000 -- (-669.423) [-666.706] (-666.118) (-676.973) * (-672.240) (-665.406) (-668.737) [-668.011] -- 0:01:05 755000 -- [-658.636] (-679.240) (-669.265) (-664.710) * (-672.072) (-660.337) (-668.432) [-665.623] -- 0:01:05 Average standard deviation of split frequencies: 0.008205 756000 -- (-666.425) (-672.946) (-672.652) [-663.757] * [-668.411] (-661.318) (-666.163) (-668.040) -- 0:01:05 757000 -- (-667.782) (-666.136) [-662.194] (-664.319) * (-667.275) [-662.026] (-661.556) (-667.716) -- 0:01:05 758000 -- (-669.155) [-664.851] (-673.617) (-665.646) * (-666.208) (-663.821) [-659.645] (-670.506) -- 0:01:04 759000 -- (-672.047) (-672.560) (-670.371) [-665.721] * (-674.383) (-662.331) [-667.251] (-669.191) -- 0:01:04 760000 -- (-668.576) (-669.127) (-670.484) [-665.643] * [-662.506] (-668.446) (-668.737) (-665.710) -- 0:01:04 Average standard deviation of split frequencies: 0.008122 761000 -- (-675.553) [-668.541] (-676.308) (-673.703) * (-667.733) [-673.354] (-669.927) (-665.465) -- 0:01:04 762000 -- (-664.509) (-676.194) [-662.347] (-661.521) * (-668.954) (-669.465) [-664.920] (-676.359) -- 0:01:03 763000 -- [-666.355] (-671.999) (-663.043) (-667.748) * [-664.577] (-665.557) (-674.326) (-667.636) -- 0:01:03 764000 -- (-662.938) [-666.942] (-666.566) (-671.303) * (-666.316) (-670.925) [-664.802] (-672.771) -- 0:01:03 765000 -- (-674.276) (-664.562) [-666.911] (-664.242) * (-665.073) [-671.372] (-665.802) (-677.950) -- 0:01:02 Average standard deviation of split frequencies: 0.007676 766000 -- (-665.776) [-661.543] (-674.522) (-667.054) * (-665.537) [-665.679] (-664.894) (-675.710) -- 0:01:02 767000 -- (-661.766) (-670.110) (-665.256) [-661.060] * (-663.465) [-666.339] (-663.939) (-673.638) -- 0:01:02 768000 -- (-671.482) (-667.968) (-665.856) [-666.745] * (-662.934) (-676.552) [-667.479] (-671.857) -- 0:01:02 769000 -- (-670.333) (-670.654) [-672.391] (-666.187) * [-659.456] (-668.324) (-663.073) (-672.145) -- 0:01:01 770000 -- [-667.945] (-665.672) (-672.962) (-662.352) * (-662.852) (-664.708) (-660.239) [-667.124] -- 0:01:01 Average standard deviation of split frequencies: 0.007630 771000 -- [-668.073] (-671.186) (-664.607) (-669.276) * (-677.323) (-666.011) [-666.814] (-663.606) -- 0:01:01 772000 -- (-670.095) (-671.056) (-662.160) [-678.618] * (-660.850) [-664.227] (-664.842) (-668.332) -- 0:01:01 773000 -- (-663.579) (-671.946) [-665.402] (-667.048) * (-663.818) [-666.056] (-669.312) (-676.643) -- 0:01:01 774000 -- (-665.928) (-661.329) (-671.329) [-663.014] * (-670.101) (-673.899) [-669.064] (-669.859) -- 0:01:00 775000 -- [-665.151] (-668.831) (-671.651) (-669.614) * (-667.528) [-669.426] (-661.381) (-668.687) -- 0:01:00 Average standard deviation of split frequencies: 0.007418 776000 -- (-675.522) (-672.362) [-664.950] (-666.808) * (-679.152) [-665.085] (-679.326) (-673.637) -- 0:01:00 777000 -- (-675.144) (-664.962) (-665.851) [-666.939] * (-670.735) (-663.119) (-670.684) [-666.148] -- 0:00:59 778000 -- (-678.911) [-664.483] (-667.653) (-665.054) * (-665.092) (-667.819) [-663.905] (-671.689) -- 0:00:59 779000 -- (-672.615) (-669.994) (-667.918) [-665.796] * [-668.249] (-663.269) (-662.817) (-659.843) -- 0:00:59 780000 -- (-666.691) (-664.266) [-662.970] (-671.899) * (-679.237) (-662.518) (-670.671) [-661.285] -- 0:00:58 Average standard deviation of split frequencies: 0.007278 781000 -- (-685.079) (-664.887) [-671.037] (-664.562) * (-667.130) (-669.183) [-676.821] (-663.534) -- 0:00:58 782000 -- (-671.883) (-675.127) (-665.821) [-666.074] * [-666.145] (-670.757) (-670.385) (-664.591) -- 0:00:58 783000 -- (-672.344) (-669.015) (-681.059) [-661.786] * (-668.838) (-664.748) (-670.436) [-662.041] -- 0:00:58 784000 -- (-666.657) [-664.477] (-664.803) (-662.524) * (-666.193) [-670.977] (-667.140) (-663.800) -- 0:00:58 785000 -- (-662.458) (-666.701) (-676.309) [-665.362] * (-671.310) [-664.164] (-670.717) (-675.419) -- 0:00:57 Average standard deviation of split frequencies: 0.007260 786000 -- (-663.439) (-670.291) [-666.318] (-660.333) * (-680.943) [-660.591] (-671.829) (-673.251) -- 0:00:57 787000 -- (-659.202) (-681.819) [-670.944] (-669.001) * (-674.785) (-663.029) (-667.893) [-675.414] -- 0:00:57 788000 -- (-663.061) (-674.177) [-661.988] (-665.682) * (-663.071) (-667.922) [-673.346] (-663.315) -- 0:00:57 789000 -- [-662.312] (-672.766) (-662.744) (-668.394) * (-672.213) (-668.062) [-663.876] (-662.823) -- 0:00:56 790000 -- (-661.852) (-668.453) [-663.471] (-671.703) * (-661.446) [-662.441] (-662.677) (-664.943) -- 0:00:56 Average standard deviation of split frequencies: 0.007274 791000 -- (-665.242) [-663.550] (-668.906) (-658.096) * [-665.563] (-667.121) (-660.227) (-671.042) -- 0:00:56 792000 -- (-669.036) (-672.995) [-671.638] (-661.914) * (-674.169) (-658.448) [-660.600] (-668.794) -- 0:00:55 793000 -- (-676.494) (-658.823) (-666.273) [-667.170] * (-665.886) [-662.187] (-658.948) (-661.718) -- 0:00:55 794000 -- (-666.705) [-670.872] (-678.789) (-663.185) * [-668.058] (-661.904) (-663.152) (-668.899) -- 0:00:55 795000 -- (-662.846) [-664.617] (-667.643) (-668.673) * [-663.511] (-660.349) (-669.204) (-661.995) -- 0:00:55 Average standard deviation of split frequencies: 0.007231 796000 -- [-661.134] (-673.886) (-663.820) (-667.840) * (-666.274) (-660.948) [-666.805] (-670.653) -- 0:00:54 797000 -- (-665.835) (-672.256) [-662.725] (-675.627) * (-667.505) (-677.825) [-660.570] (-667.756) -- 0:00:54 798000 -- (-666.327) (-674.763) [-664.492] (-663.494) * [-661.730] (-679.599) (-662.591) (-667.927) -- 0:00:54 799000 -- [-666.395] (-668.595) (-662.611) (-667.574) * (-661.622) (-665.412) (-671.156) [-667.510] -- 0:00:54 800000 -- (-668.654) (-664.500) [-662.387] (-669.517) * [-664.831] (-664.036) (-664.611) (-669.043) -- 0:00:53 Average standard deviation of split frequencies: 0.007183 801000 -- [-660.455] (-659.747) (-676.194) (-675.598) * (-669.280) (-668.239) [-663.929] (-660.267) -- 0:00:53 802000 -- (-673.298) [-665.450] (-662.755) (-665.738) * (-671.924) [-666.920] (-670.372) (-674.798) -- 0:00:53 803000 -- (-663.251) (-665.128) [-665.291] (-674.599) * [-674.708] (-672.519) (-660.632) (-663.764) -- 0:00:52 804000 -- (-665.988) (-665.960) [-667.887] (-667.411) * (-673.404) (-671.769) (-660.370) [-658.001] -- 0:00:52 805000 -- (-668.694) (-674.145) (-679.427) [-667.996] * (-672.596) (-664.863) [-660.325] (-665.246) -- 0:00:52 Average standard deviation of split frequencies: 0.006865 806000 -- (-676.120) (-672.043) [-661.404] (-674.350) * [-668.932] (-662.186) (-665.072) (-667.714) -- 0:00:51 807000 -- (-671.011) (-668.409) [-664.426] (-665.588) * (-678.269) (-662.705) [-669.038] (-671.044) -- 0:00:51 808000 -- (-667.266) [-666.501] (-671.897) (-666.350) * [-663.122] (-666.522) (-674.980) (-664.148) -- 0:00:51 809000 -- [-662.559] (-664.389) (-673.044) (-674.254) * [-667.187] (-664.486) (-671.053) (-663.728) -- 0:00:51 810000 -- [-668.641] (-667.125) (-672.673) (-663.551) * (-663.692) (-675.366) (-666.991) [-665.772] -- 0:00:51 Average standard deviation of split frequencies: 0.007131 811000 -- [-670.658] (-669.709) (-667.153) (-670.105) * (-666.279) (-676.881) (-666.640) [-666.468] -- 0:00:50 812000 -- (-672.114) (-666.947) [-664.865] (-666.712) * [-662.747] (-666.796) (-667.042) (-670.728) -- 0:00:50 813000 -- (-665.725) [-660.816] (-665.619) (-658.930) * [-660.466] (-667.464) (-665.325) (-664.964) -- 0:00:50 814000 -- [-668.185] (-663.314) (-666.090) (-663.336) * (-672.838) (-661.674) (-681.494) [-666.304] -- 0:00:50 815000 -- (-668.064) [-663.317] (-671.429) (-665.749) * (-663.987) (-669.147) [-669.803] (-668.671) -- 0:00:49 Average standard deviation of split frequencies: 0.006841 816000 -- (-668.689) (-669.680) [-666.023] (-676.039) * (-667.933) [-662.214] (-667.959) (-663.393) -- 0:00:49 817000 -- (-670.143) [-665.759] (-667.022) (-662.410) * (-669.168) [-661.191] (-675.260) (-666.880) -- 0:00:49 818000 -- (-666.885) (-660.600) (-670.631) [-669.262] * [-665.371] (-666.371) (-672.382) (-661.277) -- 0:00:48 819000 -- [-668.397] (-664.150) (-665.459) (-671.408) * (-668.326) [-667.990] (-666.618) (-667.067) -- 0:00:48 820000 -- (-660.098) [-669.817] (-665.693) (-665.569) * [-665.161] (-676.050) (-671.561) (-666.013) -- 0:00:48 Average standard deviation of split frequencies: 0.006923 821000 -- (-663.122) (-666.523) [-664.996] (-677.540) * (-666.069) (-669.015) (-661.179) [-663.322] -- 0:00:48 822000 -- [-666.266] (-665.431) (-674.379) (-677.160) * [-665.835] (-664.964) (-669.868) (-669.371) -- 0:00:47 823000 -- (-667.861) (-665.117) (-666.460) [-667.500] * (-674.167) (-664.074) (-666.303) [-666.209] -- 0:00:47 824000 -- (-673.975) (-669.639) (-666.219) [-672.943] * (-668.061) (-669.009) [-666.347] (-667.300) -- 0:00:47 825000 -- (-669.328) (-674.163) (-665.627) [-664.638] * (-667.186) [-663.071] (-670.409) (-663.397) -- 0:00:47 Average standard deviation of split frequencies: 0.006939 826000 -- (-660.748) (-664.649) (-674.152) [-671.075] * (-671.177) (-662.183) [-660.057] (-673.257) -- 0:00:46 827000 -- (-675.311) [-661.290] (-659.364) (-672.097) * [-667.301] (-663.044) (-668.257) (-670.971) -- 0:00:46 828000 -- (-661.450) (-667.297) (-668.181) [-657.833] * (-665.714) (-669.052) (-663.521) [-662.796] -- 0:00:46 829000 -- [-663.977] (-665.609) (-676.334) (-667.504) * (-666.938) (-667.207) (-668.290) [-668.573] -- 0:00:45 830000 -- (-666.883) (-671.394) [-668.223] (-673.825) * (-663.645) [-665.691] (-661.040) (-673.157) -- 0:00:45 Average standard deviation of split frequencies: 0.007019 831000 -- (-662.901) (-672.283) (-664.212) [-666.838] * (-665.633) (-667.904) [-666.780] (-670.129) -- 0:00:45 832000 -- (-661.955) (-670.891) [-663.918] (-672.866) * (-663.793) (-664.024) [-666.199] (-670.950) -- 0:00:45 833000 -- (-664.954) [-659.774] (-665.582) (-665.527) * (-667.367) (-669.658) (-673.809) [-672.822] -- 0:00:44 834000 -- (-674.763) (-665.827) (-662.146) [-666.639] * (-664.847) (-659.875) (-673.986) [-671.297] -- 0:00:44 835000 -- (-667.370) (-668.522) (-679.030) [-670.251] * (-663.589) (-671.499) (-665.807) [-668.127] -- 0:00:44 Average standard deviation of split frequencies: 0.006618 836000 -- (-671.355) (-667.847) [-662.422] (-669.124) * [-663.761] (-661.220) (-677.345) (-668.988) -- 0:00:44 837000 -- (-671.929) (-670.154) (-661.738) [-668.308] * [-663.610] (-660.338) (-672.397) (-664.596) -- 0:00:43 838000 -- (-665.464) (-665.171) (-667.578) [-661.757] * [-670.038] (-667.602) (-666.443) (-665.842) -- 0:00:43 839000 -- (-661.580) [-666.735] (-666.127) (-665.791) * (-661.094) (-666.619) [-666.602] (-662.574) -- 0:00:43 840000 -- [-666.008] (-671.791) (-669.012) (-676.326) * (-663.076) [-663.221] (-664.526) (-667.947) -- 0:00:43 Average standard deviation of split frequencies: 0.006818 841000 -- (-663.887) (-681.887) [-668.200] (-672.083) * (-664.879) (-664.670) [-661.969] (-667.101) -- 0:00:42 842000 -- [-665.979] (-676.602) (-670.457) (-667.258) * (-667.354) (-673.771) (-663.318) [-661.535] -- 0:00:42 843000 -- (-670.260) (-683.538) (-661.719) [-663.025] * (-670.933) (-680.738) [-665.270] (-665.408) -- 0:00:42 844000 -- (-667.591) (-661.777) (-672.550) [-671.795] * (-670.201) (-668.440) [-663.877] (-673.169) -- 0:00:41 845000 -- (-661.878) (-666.926) [-659.177] (-665.617) * (-668.831) (-671.073) [-666.449] (-665.576) -- 0:00:41 Average standard deviation of split frequencies: 0.006659 846000 -- [-663.752] (-665.940) (-666.556) (-668.067) * (-669.469) [-670.409] (-672.690) (-674.100) -- 0:00:41 847000 -- (-673.033) (-667.195) (-662.543) [-661.068] * (-665.520) [-668.474] (-669.232) (-668.000) -- 0:00:41 848000 -- (-676.920) [-664.438] (-670.475) (-665.194) * (-663.985) (-677.549) [-666.918] (-673.236) -- 0:00:40 849000 -- [-661.286] (-664.920) (-670.565) (-669.271) * (-666.784) [-674.027] (-667.488) (-671.451) -- 0:00:40 850000 -- (-669.531) (-668.821) [-667.199] (-668.433) * (-666.963) [-667.989] (-669.279) (-671.064) -- 0:00:40 Average standard deviation of split frequencies: 0.006883 851000 -- (-674.890) [-672.233] (-673.607) (-669.376) * [-664.493] (-662.977) (-665.169) (-668.032) -- 0:00:40 852000 -- (-669.410) (-674.186) (-669.671) [-667.026] * (-671.077) (-669.845) [-663.177] (-666.274) -- 0:00:39 853000 -- (-668.433) (-667.532) (-663.424) [-665.845] * [-668.761] (-674.076) (-665.430) (-667.254) -- 0:00:39 854000 -- (-668.537) (-670.907) (-667.633) [-669.642] * (-676.237) [-668.764] (-663.208) (-665.255) -- 0:00:39 855000 -- (-673.746) (-668.399) [-663.266] (-666.792) * (-670.097) (-666.490) [-661.069] (-670.337) -- 0:00:39 Average standard deviation of split frequencies: 0.006911 856000 -- [-668.794] (-670.171) (-662.883) (-669.890) * (-670.921) [-666.275] (-662.731) (-667.581) -- 0:00:38 857000 -- [-662.361] (-690.552) (-670.772) (-662.534) * (-664.849) [-665.931] (-665.071) (-670.538) -- 0:00:38 858000 -- (-670.410) [-662.778] (-671.405) (-667.104) * (-663.112) (-667.363) (-662.829) [-669.446] -- 0:00:38 859000 -- (-670.290) (-674.275) (-670.203) [-670.360] * (-663.500) (-666.061) (-661.106) [-668.160] -- 0:00:37 860000 -- (-669.757) (-667.354) (-662.897) [-664.093] * (-674.285) [-670.855] (-674.059) (-673.127) -- 0:00:37 Average standard deviation of split frequencies: 0.006919 861000 -- (-667.373) (-674.836) (-672.673) [-662.744] * [-660.902] (-665.351) (-664.832) (-667.321) -- 0:00:37 862000 -- (-670.964) (-676.713) (-677.426) [-666.887] * [-662.383] (-675.643) (-669.396) (-677.782) -- 0:00:37 863000 -- (-676.212) [-668.569] (-664.113) (-663.548) * (-673.307) (-668.014) [-670.742] (-671.674) -- 0:00:36 864000 -- (-665.620) (-663.395) (-662.468) [-666.403] * (-678.043) (-664.829) (-666.465) [-666.022] -- 0:00:36 865000 -- (-678.514) (-661.393) [-666.191] (-671.550) * (-670.997) [-664.905] (-670.656) (-667.758) -- 0:00:36 Average standard deviation of split frequencies: 0.006804 866000 -- (-671.816) (-661.932) [-664.661] (-668.354) * (-665.946) [-665.043] (-660.305) (-666.121) -- 0:00:36 867000 -- (-672.535) [-664.865] (-664.638) (-670.328) * [-663.769] (-664.812) (-679.358) (-678.964) -- 0:00:35 868000 -- (-679.897) (-676.688) (-669.959) [-670.649] * (-670.316) (-663.721) [-661.811] (-674.386) -- 0:00:35 869000 -- [-670.728] (-664.032) (-672.135) (-673.933) * (-666.576) (-669.627) (-660.049) [-669.070] -- 0:00:35 870000 -- (-665.953) (-671.394) [-663.479] (-663.258) * (-661.810) (-672.970) [-666.143] (-676.320) -- 0:00:34 Average standard deviation of split frequencies: 0.006383 871000 -- (-664.014) [-665.061] (-663.409) (-668.982) * (-668.986) (-666.921) [-667.902] (-659.642) -- 0:00:34 872000 -- (-674.178) (-665.883) [-673.510] (-665.558) * [-663.857] (-667.209) (-664.387) (-669.299) -- 0:00:34 873000 -- (-672.835) (-667.767) (-663.020) [-664.790] * (-661.132) (-669.103) (-675.795) [-665.751] -- 0:00:34 874000 -- (-666.647) (-668.398) (-669.141) [-668.784] * [-665.512] (-664.418) (-665.229) (-665.269) -- 0:00:33 875000 -- (-670.430) (-664.257) (-670.334) [-663.324] * [-663.639] (-667.589) (-667.444) (-662.711) -- 0:00:33 Average standard deviation of split frequencies: 0.006288 876000 -- (-670.084) (-676.892) (-668.137) [-668.100] * (-659.394) [-677.942] (-668.463) (-671.890) -- 0:00:33 877000 -- (-665.484) (-671.723) [-667.206] (-671.279) * [-663.337] (-664.492) (-670.098) (-672.266) -- 0:00:33 878000 -- (-663.732) (-672.771) (-672.372) [-664.539] * (-667.216) (-668.571) (-671.794) [-665.483] -- 0:00:32 879000 -- [-659.775] (-667.593) (-665.586) (-689.352) * (-672.397) (-669.040) (-660.922) [-666.628] -- 0:00:32 880000 -- [-668.697] (-668.625) (-672.862) (-668.004) * (-668.843) [-668.931] (-679.210) (-663.445) -- 0:00:32 Average standard deviation of split frequencies: 0.006198 881000 -- [-666.587] (-665.260) (-668.684) (-668.230) * (-668.384) (-666.744) (-675.406) [-659.751] -- 0:00:32 882000 -- [-669.745] (-666.893) (-672.951) (-667.299) * (-662.648) (-661.992) [-663.083] (-666.532) -- 0:00:31 883000 -- [-667.682] (-672.492) (-659.040) (-671.237) * [-661.663] (-681.908) (-668.494) (-666.835) -- 0:00:31 884000 -- [-658.544] (-664.970) (-670.339) (-668.134) * [-663.287] (-663.308) (-668.702) (-674.813) -- 0:00:31 885000 -- (-662.975) (-670.926) [-660.278] (-664.817) * [-663.424] (-668.543) (-666.638) (-662.987) -- 0:00:30 Average standard deviation of split frequencies: 0.006065 886000 -- (-665.991) (-677.444) (-674.177) [-664.311] * (-663.362) (-664.771) [-668.696] (-670.288) -- 0:00:30 887000 -- (-666.289) (-670.904) (-665.889) [-669.526] * (-663.911) [-668.020] (-669.318) (-665.822) -- 0:00:30 888000 -- (-668.051) (-670.997) [-657.859] (-665.089) * (-665.396) [-664.928] (-665.648) (-662.135) -- 0:00:30 889000 -- [-661.411] (-669.089) (-672.093) (-666.831) * (-665.831) (-671.704) (-668.568) [-664.024] -- 0:00:29 890000 -- (-666.140) [-665.444] (-666.888) (-672.758) * (-667.185) [-660.230] (-664.989) (-668.280) -- 0:00:29 Average standard deviation of split frequencies: 0.005954 891000 -- [-666.109] (-674.233) (-665.786) (-659.400) * (-665.984) [-670.356] (-676.010) (-682.104) -- 0:00:29 892000 -- [-672.381] (-669.516) (-679.004) (-668.623) * (-664.129) (-667.065) (-678.462) [-666.076] -- 0:00:29 893000 -- (-665.349) [-667.292] (-679.356) (-666.257) * (-669.597) [-663.662] (-669.310) (-667.233) -- 0:00:28 894000 -- [-661.591] (-665.908) (-668.010) (-668.741) * (-667.259) [-667.093] (-670.050) (-661.131) -- 0:00:28 895000 -- (-668.706) [-666.248] (-668.345) (-666.900) * (-666.442) (-660.612) [-664.095] (-675.581) -- 0:00:28 Average standard deviation of split frequencies: 0.005870 896000 -- (-667.765) [-666.948] (-670.646) (-670.240) * (-672.413) [-664.240] (-660.498) (-668.927) -- 0:00:27 897000 -- [-660.999] (-666.036) (-679.085) (-669.174) * (-667.676) (-659.670) [-666.542] (-669.374) -- 0:00:27 898000 -- (-668.331) (-665.497) [-663.625] (-674.977) * (-670.987) (-674.966) [-660.690] (-666.119) -- 0:00:27 899000 -- (-668.215) (-666.288) [-664.224] (-666.868) * (-674.161) (-683.109) (-662.434) [-664.621] -- 0:00:27 900000 -- [-671.877] (-680.440) (-663.615) (-675.092) * (-671.773) (-678.683) [-663.759] (-669.906) -- 0:00:26 Average standard deviation of split frequencies: 0.005812 901000 -- [-671.915] (-667.875) (-660.641) (-670.008) * (-663.380) (-678.279) [-665.186] (-680.353) -- 0:00:26 902000 -- (-664.910) (-669.376) [-667.364] (-666.173) * (-675.319) [-664.053] (-669.183) (-666.714) -- 0:00:26 903000 -- (-668.957) (-663.415) [-662.303] (-665.628) * [-667.231] (-666.261) (-667.339) (-667.979) -- 0:00:26 904000 -- (-672.227) (-671.169) [-662.905] (-666.677) * (-662.786) [-663.478] (-670.030) (-673.483) -- 0:00:25 905000 -- (-668.971) (-677.024) (-666.778) [-671.707] * (-665.319) (-667.651) [-668.860] (-674.693) -- 0:00:25 Average standard deviation of split frequencies: 0.005593 906000 -- (-666.934) (-662.261) (-664.611) [-662.353] * (-670.714) (-674.305) [-668.969] (-662.554) -- 0:00:25 907000 -- (-667.652) (-674.002) (-673.433) [-672.271] * (-666.355) [-664.160] (-659.810) (-673.059) -- 0:00:25 908000 -- (-673.924) (-669.133) [-670.145] (-663.026) * (-668.313) (-662.069) [-678.223] (-665.242) -- 0:00:24 909000 -- (-661.201) (-667.857) [-660.920] (-664.540) * (-670.883) (-674.937) [-658.786] (-666.312) -- 0:00:24 910000 -- (-670.450) (-664.553) (-674.917) [-666.337] * [-664.578] (-672.304) (-665.651) (-662.153) -- 0:00:24 Average standard deviation of split frequencies: 0.005720 911000 -- (-673.928) (-670.952) (-665.986) [-663.290] * (-663.685) (-673.911) (-662.701) [-667.414] -- 0:00:23 912000 -- (-666.220) (-668.796) (-665.201) [-662.846] * [-675.017] (-665.751) (-670.516) (-663.974) -- 0:00:23 913000 -- (-663.495) (-665.118) [-664.875] (-659.432) * (-664.219) (-670.645) [-670.600] (-667.189) -- 0:00:23 914000 -- (-659.477) [-661.531] (-659.312) (-678.028) * [-663.504] (-667.638) (-661.492) (-674.725) -- 0:00:23 915000 -- (-669.770) (-665.209) (-662.510) [-665.869] * (-660.981) [-671.411] (-667.055) (-679.509) -- 0:00:22 Average standard deviation of split frequencies: 0.005635 916000 -- (-661.590) (-669.652) [-658.143] (-664.881) * (-665.333) [-669.385] (-669.997) (-670.503) -- 0:00:22 917000 -- (-662.945) (-667.271) [-668.648] (-668.271) * (-676.001) (-665.866) (-664.362) [-667.124] -- 0:00:22 918000 -- [-663.475] (-665.417) (-685.799) (-664.730) * (-669.389) (-667.740) (-674.128) [-663.801] -- 0:00:22 919000 -- (-672.044) (-672.591) (-667.681) [-668.730] * (-664.998) [-657.612] (-670.812) (-666.407) -- 0:00:21 920000 -- (-669.536) [-667.305] (-667.133) (-661.195) * (-670.545) [-665.129] (-672.338) (-665.949) -- 0:00:21 Average standard deviation of split frequencies: 0.005453 921000 -- (-673.530) (-672.157) [-664.146] (-664.196) * (-669.027) [-669.421] (-662.323) (-662.785) -- 0:00:21 922000 -- (-672.563) (-667.695) [-671.005] (-673.803) * (-668.318) [-657.815] (-669.710) (-664.505) -- 0:00:20 923000 -- (-662.929) [-662.061] (-672.368) (-675.235) * (-668.498) (-671.887) [-664.728] (-668.903) -- 0:00:20 924000 -- (-669.499) [-670.201] (-668.887) (-661.959) * (-672.086) (-670.980) [-671.173] (-664.276) -- 0:00:20 925000 -- (-666.711) (-663.668) (-671.987) [-665.209] * (-667.123) [-665.647] (-670.367) (-670.511) -- 0:00:20 Average standard deviation of split frequencies: 0.005320 926000 -- [-662.562] (-667.168) (-671.932) (-665.377) * (-661.223) (-672.455) (-662.074) [-667.122] -- 0:00:19 927000 -- (-666.338) [-672.343] (-666.954) (-679.403) * (-665.394) (-670.292) [-669.102] (-667.524) -- 0:00:19 928000 -- (-665.362) (-667.854) [-665.696] (-672.236) * [-664.591] (-673.365) (-670.404) (-671.594) -- 0:00:19 929000 -- [-666.756] (-669.600) (-663.875) (-663.706) * (-666.959) [-668.853] (-666.238) (-664.496) -- 0:00:19 930000 -- (-673.364) (-668.167) [-667.473] (-669.717) * (-661.778) [-663.766] (-671.294) (-671.677) -- 0:00:18 Average standard deviation of split frequencies: 0.005015 931000 -- (-666.821) (-662.251) [-662.263] (-666.554) * (-668.824) [-669.878] (-667.401) (-673.533) -- 0:00:18 932000 -- [-673.581] (-663.185) (-664.519) (-667.317) * (-668.954) (-674.695) [-664.344] (-676.465) -- 0:00:18 933000 -- (-674.856) [-666.745] (-673.885) (-675.055) * (-676.158) [-663.243] (-672.409) (-672.630) -- 0:00:18 934000 -- (-669.930) [-658.147] (-674.235) (-667.159) * (-671.060) (-660.047) (-661.681) [-665.979] -- 0:00:17 935000 -- (-673.042) (-665.621) (-660.307) [-666.075] * [-664.401] (-666.720) (-670.490) (-674.830) -- 0:00:17 Average standard deviation of split frequencies: 0.004798 936000 -- (-669.032) (-665.646) (-666.146) [-668.024] * (-662.826) (-664.037) [-659.471] (-672.843) -- 0:00:17 937000 -- (-670.007) (-667.282) [-670.589] (-667.452) * (-662.729) (-669.162) (-670.559) [-671.573] -- 0:00:16 938000 -- (-666.840) [-668.706] (-672.413) (-660.602) * (-663.164) [-660.869] (-665.362) (-672.451) -- 0:00:16 939000 -- (-667.656) [-668.210] (-674.642) (-666.876) * (-676.427) (-667.603) (-668.533) [-659.931] -- 0:00:16 940000 -- [-661.489] (-664.743) (-672.447) (-671.354) * [-674.772] (-671.258) (-670.928) (-667.100) -- 0:00:16 Average standard deviation of split frequencies: 0.004774 941000 -- (-661.903) (-667.067) [-667.591] (-669.417) * (-664.979) (-671.874) (-661.124) [-664.634] -- 0:00:15 942000 -- (-678.366) (-665.817) (-674.254) [-662.758] * (-670.024) (-664.678) (-670.667) [-671.329] -- 0:00:15 943000 -- [-668.208] (-665.226) (-667.924) (-664.808) * (-677.842) (-667.070) [-668.589] (-665.814) -- 0:00:15 944000 -- (-667.938) (-666.951) [-664.975] (-672.729) * [-669.051] (-662.843) (-665.910) (-668.791) -- 0:00:15 945000 -- (-662.381) (-676.173) (-679.873) [-661.425] * (-666.774) [-661.158] (-663.430) (-668.758) -- 0:00:14 Average standard deviation of split frequencies: 0.004695 946000 -- (-668.928) (-675.658) [-674.371] (-665.505) * (-668.159) (-667.064) [-664.228] (-666.352) -- 0:00:14 947000 -- [-663.409] (-684.117) (-666.990) (-670.804) * (-673.921) [-672.913] (-667.259) (-669.434) -- 0:00:14 948000 -- (-665.124) [-659.507] (-666.332) (-671.720) * (-669.746) (-664.971) (-663.897) [-667.631] -- 0:00:13 949000 -- [-669.779] (-671.505) (-670.059) (-661.338) * [-667.838] (-670.035) (-668.734) (-665.186) -- 0:00:13 950000 -- (-673.403) (-667.802) (-664.078) [-666.531] * (-675.437) [-670.224] (-665.475) (-660.545) -- 0:00:13 Average standard deviation of split frequencies: 0.004959 951000 -- (-666.458) (-667.365) [-664.087] (-669.534) * (-668.594) [-664.395] (-667.841) (-665.212) -- 0:00:13 952000 -- (-667.784) (-661.663) (-670.174) [-666.006] * (-678.820) (-663.555) (-665.552) [-668.793] -- 0:00:12 953000 -- [-666.451] (-677.386) (-676.159) (-681.558) * [-662.921] (-666.065) (-671.523) (-672.408) -- 0:00:12 954000 -- (-667.574) (-669.377) [-662.025] (-676.043) * [-672.842] (-666.504) (-663.598) (-675.904) -- 0:00:12 955000 -- (-669.809) (-665.664) [-670.762] (-668.421) * [-667.294] (-668.659) (-669.594) (-672.628) -- 0:00:12 Average standard deviation of split frequencies: 0.004905 956000 -- (-662.571) (-662.504) [-667.135] (-664.613) * (-673.213) (-674.276) (-669.381) [-671.032] -- 0:00:11 957000 -- [-662.975] (-670.568) (-675.667) (-666.536) * [-662.853] (-668.502) (-668.229) (-669.177) -- 0:00:11 958000 -- (-662.008) [-665.310] (-670.163) (-660.702) * [-660.082] (-672.982) (-673.829) (-668.261) -- 0:00:11 959000 -- (-675.932) (-670.026) (-675.186) [-664.190] * (-679.630) (-665.524) [-664.558] (-668.947) -- 0:00:11 960000 -- (-672.415) [-666.338] (-669.789) (-662.810) * (-671.727) (-661.272) (-673.847) [-668.137] -- 0:00:10 Average standard deviation of split frequencies: 0.005165 961000 -- (-672.809) (-672.586) (-665.887) [-661.483] * [-673.334] (-665.213) (-666.124) (-669.906) -- 0:00:10 962000 -- (-676.610) (-666.924) (-669.259) [-661.765] * (-667.669) (-672.754) [-660.268] (-666.304) -- 0:00:10 963000 -- [-671.287] (-669.416) (-673.426) (-660.330) * (-665.680) [-678.974] (-669.908) (-674.352) -- 0:00:09 964000 -- (-671.913) (-662.450) (-671.911) [-663.405] * [-664.910] (-671.031) (-669.041) (-664.819) -- 0:00:09 965000 -- (-683.291) [-662.477] (-666.777) (-675.945) * (-682.475) [-664.697] (-677.999) (-672.364) -- 0:00:09 Average standard deviation of split frequencies: 0.005111 966000 -- (-660.019) (-665.888) [-659.786] (-676.011) * (-667.169) [-663.915] (-679.226) (-661.342) -- 0:00:09 967000 -- (-669.204) (-668.183) (-669.623) [-669.058] * (-671.629) [-662.650] (-676.365) (-668.989) -- 0:00:08 968000 -- (-668.608) [-673.302] (-664.656) (-663.215) * (-660.871) [-668.500] (-672.450) (-668.243) -- 0:00:08 969000 -- [-664.311] (-667.756) (-669.309) (-668.124) * (-661.221) (-665.565) [-673.245] (-660.830) -- 0:00:08 970000 -- (-668.619) [-669.530] (-668.827) (-662.391) * (-669.228) (-673.137) (-671.699) [-665.159] -- 0:00:08 Average standard deviation of split frequencies: 0.004959 971000 -- (-676.304) (-669.344) (-667.552) [-664.553] * (-665.992) (-672.799) [-669.890] (-665.831) -- 0:00:07 972000 -- (-670.953) [-668.703] (-667.226) (-669.892) * [-667.855] (-668.483) (-665.804) (-658.098) -- 0:00:07 973000 -- (-663.613) (-670.101) [-668.110] (-670.634) * (-665.049) [-670.898] (-674.537) (-666.440) -- 0:00:07 974000 -- (-671.731) (-671.297) [-663.000] (-681.051) * (-667.279) (-668.301) (-667.720) [-662.600] -- 0:00:06 975000 -- [-664.623] (-668.789) (-665.875) (-680.629) * (-665.741) [-670.191] (-670.382) (-669.302) -- 0:00:06 Average standard deviation of split frequencies: 0.005059 976000 -- (-670.789) (-663.379) (-662.678) [-670.673] * (-671.043) (-663.635) (-671.134) [-662.674] -- 0:00:06 977000 -- (-666.724) (-672.956) (-672.726) [-662.141] * (-661.828) [-662.963] (-668.983) (-675.207) -- 0:00:06 978000 -- [-666.020] (-667.389) (-675.682) (-674.115) * (-671.090) (-661.348) (-666.262) [-669.235] -- 0:00:05 979000 -- (-663.038) [-672.864] (-673.159) (-669.490) * (-670.058) (-658.155) (-662.211) [-667.795] -- 0:00:05 980000 -- (-671.471) (-667.806) [-666.169] (-660.132) * (-664.354) (-662.582) [-670.976] (-662.001) -- 0:00:05 Average standard deviation of split frequencies: 0.004908 981000 -- (-672.264) [-663.434] (-664.085) (-660.543) * (-665.591) (-663.951) [-663.382] (-666.347) -- 0:00:05 982000 -- [-668.582] (-662.679) (-674.999) (-667.047) * (-676.040) (-675.240) [-662.326] (-665.743) -- 0:00:04 983000 -- [-667.102] (-674.727) (-666.242) (-668.783) * (-681.147) [-670.345] (-663.814) (-668.189) -- 0:00:04 984000 -- (-676.281) (-669.365) (-666.301) [-664.554] * [-672.500] (-677.173) (-667.598) (-672.963) -- 0:00:04 985000 -- (-667.415) (-673.238) [-666.937] (-666.690) * [-668.065] (-663.621) (-660.457) (-664.905) -- 0:00:04 Average standard deviation of split frequencies: 0.004982 986000 -- (-661.841) (-674.556) (-659.516) [-671.273] * [-666.754] (-667.316) (-676.639) (-670.172) -- 0:00:03 987000 -- (-662.464) [-670.997] (-659.997) (-670.767) * [-672.181] (-676.673) (-669.102) (-671.421) -- 0:00:03 988000 -- [-660.301] (-670.420) (-675.475) (-666.633) * (-667.915) (-678.713) (-670.227) [-670.631] -- 0:00:03 989000 -- (-675.081) (-663.952) (-670.503) [-672.260] * (-663.970) (-670.984) (-671.197) [-665.614] -- 0:00:02 990000 -- (-672.435) (-672.676) [-663.665] (-677.223) * (-665.092) (-676.353) [-675.184] (-673.348) -- 0:00:02 Average standard deviation of split frequencies: 0.005209 991000 -- (-671.746) (-662.590) [-664.221] (-673.136) * (-670.684) [-665.755] (-670.624) (-663.640) -- 0:00:02 992000 -- [-665.996] (-666.624) (-669.736) (-669.612) * (-670.034) [-665.953] (-668.499) (-670.578) -- 0:00:02 993000 -- (-673.572) (-665.164) (-674.313) [-663.374] * (-667.662) (-668.920) (-672.900) [-668.583] -- 0:00:01 994000 -- (-665.380) (-667.985) [-663.306] (-667.671) * (-672.555) [-666.486] (-675.105) (-662.229) -- 0:00:01 995000 -- (-673.296) [-661.658] (-664.314) (-673.703) * (-665.495) (-671.906) (-662.929) [-671.333] -- 0:00:01 Average standard deviation of split frequencies: 0.005156 996000 -- [-667.376] (-675.466) (-672.137) (-668.227) * (-668.826) (-674.637) (-667.713) [-666.930] -- 0:00:01 997000 -- (-662.552) [-668.005] (-663.455) (-671.009) * (-668.071) [-665.921] (-673.932) (-667.298) -- 0:00:00 998000 -- (-664.914) (-664.780) [-665.003] (-666.944) * (-664.138) [-662.927] (-667.830) (-680.955) -- 0:00:00 999000 -- [-671.985] (-663.890) (-668.751) (-673.279) * (-668.814) [-658.726] (-674.036) (-670.421) -- 0:00:00 1000000 -- [-667.948] (-663.694) (-664.118) (-665.105) * (-669.234) (-669.298) [-666.262] (-665.341) -- 0:00:00 Average standard deviation of split frequencies: 0.005356 Analysis completed in 4 mins 29 seconds Analysis used 269.03 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -653.97 Likelihood of best state for "cold" chain of run 2 was -654.18 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 67.9 % ( 59 %) Dirichlet(Revmat{all}) 81.2 % ( 75 %) Slider(Revmat{all}) 39.6 % ( 31 %) Dirichlet(Pi{all}) 39.1 % ( 22 %) Slider(Pi{all}) 73.8 % ( 60 %) Multiplier(Alpha{1,2}) 66.2 % ( 43 %) Multiplier(Alpha{3}) 87.1 % ( 75 %) Slider(Pinvar{all}) 40.7 % ( 36 %) ExtSPR(Tau{all},V{all}) 31.0 % ( 30 %) ExtTBR(Tau{all},V{all}) 42.7 % ( 43 %) NNI(Tau{all},V{all}) 44.3 % ( 43 %) ParsSPR(Tau{all},V{all}) 27.2 % ( 21 %) Multiplier(V{all}) 57.7 % ( 51 %) Nodeslider(V{all}) 26.3 % ( 28 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 68.6 % ( 66 %) Dirichlet(Revmat{all}) 80.7 % ( 69 %) Slider(Revmat{all}) 40.2 % ( 30 %) Dirichlet(Pi{all}) 38.7 % ( 24 %) Slider(Pi{all}) 73.5 % ( 47 %) Multiplier(Alpha{1,2}) 66.6 % ( 41 %) Multiplier(Alpha{3}) 86.5 % ( 67 %) Slider(Pinvar{all}) 40.5 % ( 45 %) ExtSPR(Tau{all},V{all}) 30.8 % ( 35 %) ExtTBR(Tau{all},V{all}) 42.8 % ( 47 %) NNI(Tau{all},V{all}) 44.3 % ( 44 %) ParsSPR(Tau{all},V{all}) 27.2 % ( 23 %) Multiplier(V{all}) 57.9 % ( 70 %) Nodeslider(V{all}) 26.3 % ( 31 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.75 0.53 0.37 2 | 166434 0.76 0.57 3 | 166846 166956 0.78 4 | 166953 166597 166214 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.75 0.53 0.37 2 | 166499 0.77 0.57 3 | 166757 166666 0.78 4 | 166674 166801 166603 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -664.25 | 2 1 1 2 1 | | 1 2 2 1 | | 1 1 2 1 * | | 2 2 2 2 12 22 | | 2 1 2 1 1 1 * 1 1| | 1 2 1 22 1 1 2 21 2 * 2| |2*11 1 2 2 1 2 22 * 2 2 1 | |1 1 2 1 1 2 2 2 1* 1 22 2 | | 2 * 1 2 112 1 1 1 1 1 1 1 1 1 1 | | 22 1 11 2 122 1 1 | | 2 2 2 22 | | 2 2 1 1 2 | | | | | | 1 2 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -668.13 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -661.42 -674.27 2 -661.39 -674.79 -------------------------------------- TOTAL -661.41 -674.56 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.243776 0.001885 0.157868 0.326228 0.239299 1101.06 1246.22 1.000 r(A<->C){all} 0.125960 0.002750 0.031124 0.230342 0.121468 510.00 557.36 1.001 r(A<->G){all} 0.155073 0.003314 0.052039 0.265862 0.149024 503.52 583.70 1.000 r(A<->T){all} 0.109110 0.001481 0.039763 0.184046 0.105104 591.45 708.85 1.000 r(C<->G){all} 0.192989 0.004551 0.068097 0.322612 0.188350 500.86 507.06 1.000 r(C<->T){all} 0.303893 0.004489 0.170312 0.427833 0.299457 585.66 666.18 1.000 r(G<->T){all} 0.112975 0.001934 0.030100 0.194180 0.108138 587.04 764.06 1.000 pi(A){all} 0.255018 0.000593 0.209766 0.302909 0.254409 1023.33 1097.78 1.000 pi(C){all} 0.197026 0.000478 0.156667 0.239690 0.196978 1032.38 1187.69 1.000 pi(G){all} 0.177421 0.000461 0.135303 0.218348 0.176994 1301.74 1337.95 1.000 pi(T){all} 0.370535 0.000718 0.321220 0.423978 0.370215 1163.42 1178.75 1.000 alpha{1,2} 0.682364 0.518946 0.000092 2.031786 0.447306 898.44 1085.52 1.000 alpha{3} 1.950309 1.446788 0.318461 4.416256 1.656120 1429.24 1449.96 1.001 pinvar{all} 0.189105 0.017979 0.000035 0.437507 0.168981 1173.77 1222.88 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C10 3 -- C2 4 -- C3 5 -- C4 6 -- C5 7 -- C6 8 -- C7 9 -- C8 10 -- C9 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition ---------------- 1 -- .********* 2 -- .*........ 3 -- ..*....... 4 -- ...*...... 5 -- ....*..... 6 -- .....*.... 7 -- ......*... 8 -- .......*.. 9 -- ........*. 10 -- .........* 11 -- .....**... 12 -- .*......*. 13 -- ....***... 14 -- .*..***.*. 15 -- .*.******* 16 -- .*.****.*. 17 -- .******.** 18 -- .*..***.** 19 -- ..*....*.. 20 -- .......*.* 21 -- .*..*****. 22 -- ..*......* 23 -- ...*...*.. 24 -- .**.***.*. 25 -- .**.****** 26 -- ...*.....* 27 -- ..**...*.* 28 -- .********. 29 -- ..**...... ---------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 11 3002 1.000000 0.000000 1.000000 1.000000 2 12 3002 1.000000 0.000000 1.000000 1.000000 2 13 3002 1.000000 0.000000 1.000000 1.000000 2 14 3002 1.000000 0.000000 1.000000 1.000000 2 15 473 0.157562 0.011777 0.149234 0.165889 2 16 455 0.151566 0.001413 0.150566 0.152565 2 17 450 0.149900 0.002827 0.147901 0.151899 2 18 449 0.149567 0.008009 0.143904 0.155230 2 19 443 0.147568 0.008951 0.141239 0.153897 2 20 433 0.144237 0.001413 0.143238 0.145237 2 21 431 0.143571 0.002355 0.141905 0.145237 2 22 430 0.143238 0.000942 0.142572 0.143904 2 23 429 0.142905 0.014604 0.132578 0.153231 2 24 428 0.142572 0.003769 0.139907 0.145237 2 25 425 0.141572 0.008009 0.135909 0.147235 2 26 422 0.140573 0.001884 0.139241 0.141905 2 27 414 0.137908 0.010364 0.130580 0.145237 2 28 409 0.136243 0.014604 0.125916 0.146569 2 29 393 0.130913 0.010835 0.123251 0.138574 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.003069 0.000009 0.000000 0.008913 0.002186 1.000 2 length{all}[2] 0.013603 0.000055 0.001843 0.027856 0.012274 1.000 2 length{all}[3] 0.003194 0.000011 0.000001 0.009734 0.002225 1.000 2 length{all}[4] 0.003159 0.000010 0.000000 0.009455 0.002206 1.000 2 length{all}[5] 0.004447 0.000018 0.000001 0.012584 0.003212 1.000 2 length{all}[6] 0.022455 0.000085 0.007595 0.041212 0.020970 1.000 2 length{all}[7] 0.003266 0.000010 0.000000 0.009666 0.002241 1.000 2 length{all}[8] 0.003019 0.000010 0.000000 0.009118 0.002084 1.000 2 length{all}[9] 0.005698 0.000027 0.000003 0.015491 0.004332 1.001 2 length{all}[10] 0.003082 0.000009 0.000001 0.009390 0.002133 1.000 2 length{all}[11] 0.033139 0.000137 0.012908 0.056294 0.031517 1.000 2 length{all}[12] 0.034790 0.000188 0.011482 0.062509 0.032885 1.000 2 length{all}[13] 0.061158 0.000358 0.027746 0.098241 0.058905 1.000 2 length{all}[14] 0.040388 0.000208 0.016026 0.070780 0.038265 1.000 2 length{all}[15] 0.002909 0.000010 0.000004 0.008961 0.001998 0.998 2 length{all}[16] 0.003123 0.000010 0.000004 0.010369 0.002125 0.998 2 length{all}[17] 0.003028 0.000011 0.000004 0.009252 0.002031 0.998 2 length{all}[18] 0.003150 0.000011 0.000003 0.009585 0.002078 1.000 2 length{all}[19] 0.003290 0.000011 0.000013 0.010239 0.002273 0.998 2 length{all}[20] 0.003296 0.000010 0.000002 0.009265 0.002317 0.998 2 length{all}[21] 0.003114 0.000008 0.000025 0.008940 0.002362 1.001 2 length{all}[22] 0.002989 0.000009 0.000001 0.009557 0.001844 1.004 2 length{all}[23] 0.003214 0.000013 0.000002 0.010061 0.002117 0.998 2 length{all}[24] 0.003415 0.000012 0.000010 0.009913 0.002435 1.004 2 length{all}[25] 0.002874 0.000009 0.000009 0.009164 0.001947 1.005 2 length{all}[26] 0.003332 0.000012 0.000029 0.009958 0.002270 0.999 2 length{all}[27] 0.002846 0.000010 0.000003 0.009165 0.001887 1.001 2 length{all}[28] 0.003121 0.000010 0.000029 0.009366 0.002215 1.000 2 length{all}[29] 0.003179 0.000009 0.000005 0.009676 0.002222 0.998 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.005356 Maximum standard deviation of split frequencies = 0.014604 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.005 Clade credibility values: /----------------------------------------------------------------------- C1 (1) | |----------------------------------------------------------------------- C2 (3) | |----------------------------------------------------------------------- C3 (4) | |----------------------------------------------------------------------- C7 (8) + |----------------------------------------------------------------------- C9 (10) | | /------------------ C10 (2) | /----------------100---------------+ | | \------------------ C8 (9) | | \-------100-------+ /----------------------------------- C4 (5) | | \-------100-------+ /------------------ C5 (6) \-------100------+ \------------------ C6 (7) Phylogram (based on average branch lengths): /- C1 (1) | |- C2 (3) | |- C3 (4) | |- C7 (8) + |- C9 (10) | | /------ C10 (2) | /---------------+ | | \-- C8 (9) | | \-----------------+ /- C4 (5) | | \----------------------------+ /---------- C5 (6) \--------------+ \- C6 (7) |--------| 0.020 expected changes per site Calculating tree probabilities... Credible sets of trees (105 trees sampled): 50 % credible set contains 45 trees 90 % credible set contains 91 trees 95 % credible set contains 98 trees 99 % credible set contains 104 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,C3,C7,C9,((C10,C8),(C4,(C5,C6))))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **10** sequences, **91** codons, and **1** partitions from `/data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/results/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/results/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/results/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -652.05, AIC-c = 1348.47 (22 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.213 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 2.815 * non-synonymous rate = 0.643 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 12 | 1 | 1.252 | 9.247 | Pos. posterior = 0.9262 | ---- ## FUBAR inferred 1 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.07 are expected to be false positives (95% confidence interval of 0-1 )
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=10, Len=91 B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM B07f_NS3a_ABN10858_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM BtTp_GX2012_NA_AIA62353_1_2012_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNVTAILLVLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM CZ01_orf3_AWH65878_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ---MNVTAILLQNVANAFSNPLYVPEHCVGMPGTLFQACIRQTMVDTTGM CZ07_orf3_AWH65889_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ---MNVTAILLQNVANAFSNPLYVPEHCVGMSGTLFQACIRQTMVDTTGM HKU4_1_B04f_NS3a_YP_001039954_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM LMH1f_NS3a_ABN10867_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM SM3A_NA_QQD78084_1_2010_08_16_Hong_Kong_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU40 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSATLFQACIRQTMVDTTGM :*.***:* :*****:******** **..***************** B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV B07f_NS3a_ABN10858_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV BtTp_GX2012_NA_AIA62353_1_2012_China_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV CZ01_orf3_AWH65878_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSYDGTTIPFDIHGIVHEDHYTDTKPTPLSDVGFSV CZ07_orf3_AWH65889_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV HKU4_1_B04f_NS3a_YP_001039954_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV LMH1f_NS3a_ABN10867_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSHDGITIPFDRDGIVHEEHYTETNPTPLSDVGFSV SM3A_NA_QQD78084_1_2010_08_16_Hong_Kong_Bat_Tylonycteris_bat_coronavirus_HKU4 YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU40 YTNSDMSHDGITIPFDRDGIVHEEHYTETNPTPLFDAGFSV **** **:** ***** .*****:***:*:**** *.****
>B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTCTTCTTTTATTACTTGCTAATGCTTTTTCTAAACCTTTATATGTCCCTGAGCATTGTGGTGGCATGTCTGGCACTCTCTTTCAGGCTTGTATTAGACAAACTATGGTTGATACAACCGGTATGTACACCAATTCAGCTATGTCACATGATGGTGTTACAATACCATTTGATAGAGATGGCATTGTACATGAAGATCATTACACTGAAACAAACCCTACACCACTTTTTGATGCTGGGTTTTCCGTA >B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTCTTCTTTTATTACTTGCTAATGCTTTTTCTAAACCTTTATATGTCCCTGAGCATTGTGGTGGCATGTCTGGCACTCTCTTTCAGGCTTGTATTAGACAAACTATGGTTGATACAACCGGTATGTACACCAATTCAGCTATGTCACATGATGGTGTTACAATACCATTTGATAGAGATGGCATTGTACATGAAGATCATTACACTGAAACAAACCCTACACCACTTTTTGATGCTGGGTTTTCCGTA >B07f_NS3a_ABN10858_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTCTTCTTTTATTACTTGCTAATGCTTTTTCTAAACCTTTATATGTCCCTGAGCATTGTGGTGGCATGTCTGGCACTCTCTTTCAGGCTTGTATTAGACAAACTATGGTTGATACAACCGGTATGTACACCAATTCAGCTATGTCACATGATGGTGTTACAATACCATTTGATAGAGATGGCATTGTACATGAAGATCATTACACTGAAACAAACCCTACACCACTTTTTGATGCTGGGTTTTCCGTA >BtTp_GX2012_NA_AIA62353_1_2012_China_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAACGTTACTGCTATTCTTCTCGTATTAGTTGCTAATGCTTTTTCTAAACCCTTATATGTTCCTGAACATTGTGTTGGCATGTCTGGCACTTTGTTTCAAGCTTGTATTAGACAAACTATGGTTGATACAACAGGTATGTACACCAATTCAGCTATGTCATATGATGGTACTACAATACCATTCGATAGAGATGGCATTGTACATGAGGATCATTACACTGATACAAAACCTACACCCTTGTCTGATGTTGGTTTTTCCGTA >CZ01_orf3_AWH65878_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ---------ATGAACGTTACTGCTATTCTTCTCCAAAACGTTGCTAATGCTTTTTCTAATCCCTTATATGTTCCTGAACATTGTGTTGGCATGCCCGGGACTTTGTTTCAAGCTTGTATTAGACAAACTATGGTTGATACAACAGGTATGTACACCAATTCAGCTATGTCATATGATGGTACTACAATACCATTCGATATTCATGGCATTGTACATGAGGATCATTACACTGATACTAAACCTACACCCTTGTCTGATGTTGGTTTTTCCGTA >CZ07_orf3_AWH65889_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ---------ATGAACGTTACTGCTATTCTTCTCCAAAACGTTGCTAATGCTTTTTCTAATCCCTTATATGTTCCTGAACATTGTGTTGGCATGTCTGGCACTTTGTTTCAAGCTTGTATTAGACAAACTATGGTTGATACAACAGGTATGTACACCAATTCAGCTATGTCATATGATGGTACTACAATACCATTCGATAGAGATGGCATTGTACATGAGGATCATTACACTGATACTAAACCTACACCCTTGTCTGATGTTGGTTTTTCCGTA >HKU4_1_B04f_NS3a_YP_001039954_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTCTTCTTTTATTACTTGCTAATGCTTTTTCTAAACCTTTATATGTCCCTGAGCATTGTGGTGGCATGTCTGGCACTCTCTTTCAGGCTTGTATTAGACAAACTATGGTTGATACAACCGGTATGTACACCAATTCAGCTATGTCACATGATGGTGTTACAATACCATTTGATAGAGATGGCATTGTACATGAAGATCATTACACTGAAACAAACCCTACACCACTTTTTGATGCTGGGTTTTCCGTA >LMH1f_NS3a_ABN10867_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTTTTCTTTTATTAGTTGCTAATGCTTTTTCTAAACCATTGTATGTACCTGAACATTGTGTTGGCATGTCTGGCACTTTGTTTCAAGCTTGTATTAGACAAACTATGGTTGATACAACTGGTATGTATACCAACTCAGCTATGTCACATGATGGTATTACAATACCATTCGATAGGGATGGCATTGTACATGAAGAGCATTATACTGAAACAAACCCTACACCACTTTCTGATGTTGGGTTTTCCGTA >SM3A_NA_QQD78084_1_2010_08_16_Hong_Kong_Bat_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTCTTCTTTTATTACTTGCTAATGCTTTTTCTAAACCTTTATATGTCCCTGAGCATTGTGGTGGCATGTCTGGCACTCTCTTTCAGGCTTGTATTAGACAAACTATGGTTGATACAACCGGTATGTACACCAATTCAGCTATGTCACATGATGGTGTTACAATACCATTTGATAGAGATGGCATTGTACATGAAGATCATTACACTGAAACAAACCCTACACCACTTTTTGATGCTGGGTTTTCCGTA >SZ140324_orf3_AWH65900_1_2014_04_23_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ATGGTTTCTTTTAATGCTACTGCTATTTTTCTTTTATTAGTTGCTAATGCTTTTTCTAAACCATTGTATGTACCTGAACATTGTGTTGGCATGTCTGCCACTTTGTTTCAAGCTTGTATTAGACAAACTATGGTTGATACAACTGGTATGTATACCAACTCAGATATGTCACATGATGGTATTACAATACCATTCGATAGGGATGGCATTGTACATGAAGAGCATTATACTGAAACAAACCCTACACCACTTTTTGATGCTGGGTTTTCCGTA
>B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV >B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV >B07f_NS3a_ABN10858_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV >BtTp_GX2012_NA_AIA62353_1_2012_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNVTAILLVLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV >CZ01_orf3_AWH65878_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ---MNVTAILLQNVANAFSNPLYVPEHCVGMPGTLFQACIRQTMVDTTGM YTNSAMSYDGTTIPFDIHGIVHEDHYTDTKPTPLSDVGFSV >CZ07_orf3_AWH65889_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 ---MNVTAILLQNVANAFSNPLYVPEHCVGMSGTLFQACIRQTMVDTTGM YTNSAMSYDGTTIPFDRDGIVHEDHYTDTKPTPLSDVGFSV >HKU4_1_B04f_NS3a_YP_001039954_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV >LMH1f_NS3a_ABN10867_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSGTLFQACIRQTMVDTTGM YTNSAMSHDGITIPFDRDGIVHEEHYTETNPTPLSDVGFSV >SM3A_NA_QQD78084_1_2010_08_16_Hong_Kong_Bat_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAILLLLLANAFSKPLYVPEHCGGMSGTLFQACIRQTMVDTTGM YTNSAMSHDGVTIPFDRDGIVHEDHYTETNPTPLFDAGFSV >SZ140324_orf3_AWH65900_1_2014_04_23_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 MVSFNATAIFLLLVANAFSKPLYVPEHCVGMSATLFQACIRQTMVDTTGM YTNSDMSHDGITIPFDRDGIVHEEHYTETNPTPLFDAGFSV
Reading sequence file /data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/fasta/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1 Found 10 sequences of length 273 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 7.7% Found 41 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 12 Calculating analytical mean and variance Doing permutation test for PHI Doing permutation test for NSS Doing Permutation test for MAXCHI Writing alignment of polymorphic unambig sites to: Phi.poly.sites Window size is 32 polymorphic sites **p-Value(s)** ---------- NSS: 2.00e-02 (1000 permutations) Max Chi^2: 1.10e-02 (1000 permutations) PHI (Permutation): 3.49e-01 (1000 permutations) PHI (Normal): 2.94e-01
#NEXUS [ID: 1938929574] begin taxa; dimensions ntax=10; taxlabels B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 SZ140324_orf3_AWH65900_1_2014_04_23_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 B07f_NS3a_ABN10858_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 BtTp_GX2012_NA_AIA62353_1_2012_China_Bat_Tylonycteris_bat_coronavirus_HKU4 CZ01_orf3_AWH65878_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 CZ07_orf3_AWH65889_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4 HKU4_1_B04f_NS3a_YP_001039954_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 LMH1f_NS3a_ABN10867_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4 SM3A_NA_QQD78084_1_2010_08_16_Hong_Kong_Bat_Tylonycteris_bat_coronavirus_HKU4 ; end; begin trees; translate 1 B04f_NS3a_ABN10840_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4, 2 SZ140324_orf3_AWH65900_1_2014_04_23_China_Unknown_Tylonycteris_bat_coronavirus_HKU4, 3 B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4, 4 B07f_NS3a_ABN10858_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4, 5 BtTp_GX2012_NA_AIA62353_1_2012_China_Bat_Tylonycteris_bat_coronavirus_HKU4, 6 CZ01_orf3_AWH65878_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4, 7 CZ07_orf3_AWH65889_1_2012_11_01_China_Unknown_Tylonycteris_bat_coronavirus_HKU4, 8 HKU4_1_B04f_NS3a_YP_001039954_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4, 9 LMH1f_NS3a_ABN10867_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4, 10 SM3A_NA_QQD78084_1_2010_08_16_Hong_Kong_Bat_Tylonycteris_bat_coronavirus_HKU4 ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:2.186038e-03,3:2.224785e-03,4:2.206385e-03,8:2.083606e-03,10:2.133000e-03,((2:1.227391e-02,9:4.332192e-03)1.000:3.288507e-02,(5:3.212290e-03,(6:2.097049e-02,7:2.240621e-03)1.000:3.151663e-02)1.000:5.890539e-02)1.000:3.826451e-02); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:2.186038e-03,3:2.224785e-03,4:2.206385e-03,8:2.083606e-03,10:2.133000e-03,((2:1.227391e-02,9:4.332192e-03):3.288507e-02,(5:3.212290e-03,(6:2.097049e-02,7:2.240621e-03):3.151663e-02):5.890539e-02):3.826451e-02); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -661.20 -675.31 2 -661.42 -674.82 -------------------------------------- TOTAL -661.31 -675.09 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.244417 0.001925 0.166413 0.333500 0.241348 1347.97 1379.67 1.000 r(A<->C){all} 0.130756 0.002910 0.034473 0.232751 0.124507 547.35 611.46 1.001 r(A<->G){all} 0.151664 0.003032 0.051761 0.257343 0.147630 536.08 614.59 1.001 r(A<->T){all} 0.107938 0.001444 0.037894 0.183019 0.103776 501.22 658.85 1.000 r(C<->G){all} 0.194736 0.004460 0.070312 0.322834 0.190441 595.91 620.68 1.000 r(C<->T){all} 0.305273 0.004349 0.185833 0.437422 0.303200 678.59 692.81 1.000 r(G<->T){all} 0.109632 0.001903 0.035757 0.201501 0.104854 426.53 570.62 1.000 pi(A){all} 0.256399 0.000603 0.211168 0.305750 0.255863 1142.95 1208.62 1.000 pi(C){all} 0.196613 0.000483 0.153181 0.240209 0.196081 862.43 1000.99 1.000 pi(G){all} 0.176108 0.000482 0.133979 0.217289 0.175082 1074.12 1168.91 1.000 pi(T){all} 0.370880 0.000760 0.321745 0.426653 0.369967 1141.06 1180.20 1.000 alpha{1,2} 0.660894 0.486095 0.001693 2.010291 0.442134 1107.58 1143.40 1.001 alpha{3} 1.954139 1.360016 0.304380 4.298999 1.691177 1110.77 1305.88 1.000 pinvar{all} 0.187144 0.017825 0.000136 0.440395 0.162519 955.53 1021.50 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,C3,C7,C9,((C10,C8),(C4,(C5,C6))))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **10** sequences, **91** codons, and **1** partitions from `/data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/results/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/results/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result/original_alignment/fubar/results/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1/B05f_NS3a_ABN10849_1_NA_China_Bat_Tylonycteris_bat_coronavirus_HKU4.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -652.05, AIC-c = 1348.47 (22 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.213 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 2.815 * non-synonymous rate = 0.643 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 12 | 1 | 1.252 | 9.247 | Pos. posterior = 0.9262 | ---- ## FUBAR inferred 1 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.07 are expected to be false positives (95% confidence interval of 0-1 )
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500