--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -1138.99 -1150.11 2 -1138.92 -1151.65 -------------------------------------- TOTAL -1138.96 -1151.15 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.049991 0.000094 0.032747 0.070314 0.049216 1169.66 1237.95 1.000 r(A<->C){all} 0.057955 0.001639 0.000054 0.135203 0.048454 367.55 595.99 1.000 r(A<->G){all} 0.308498 0.006472 0.159727 0.472576 0.304598 481.72 484.15 1.000 r(A<->T){all} 0.059258 0.001149 0.007039 0.127443 0.052757 604.63 824.56 1.000 r(C<->G){all} 0.068379 0.002223 0.000070 0.162695 0.059199 366.84 470.88 1.000 r(C<->T){all} 0.430546 0.007266 0.273412 0.598583 0.427641 459.20 488.09 1.000 r(G<->T){all} 0.075364 0.001676 0.008881 0.156847 0.068968 503.68 594.86 1.000 pi(A){all} 0.249189 0.000264 0.218814 0.280954 0.249079 1105.15 1231.64 1.000 pi(C){all} 0.206031 0.000212 0.179091 0.235370 0.205697 1038.33 1079.15 1.000 pi(G){all} 0.210622 0.000231 0.181055 0.239878 0.210508 1238.34 1273.56 1.000 pi(T){all} 0.334158 0.000311 0.300140 0.368910 0.333791 1349.19 1383.28 1.000 alpha{1,2} 0.957541 0.986826 0.000033 2.874150 0.652734 1266.28 1274.06 1.000 alpha{3} 1.334892 1.154283 0.000990 3.348768 1.078726 1082.63 1153.10 1.000 pinvar{all} 0.299916 0.040714 0.000055 0.667298 0.271195 751.07 871.97 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Fri Oct 21 22:38:19 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.08 sec, SCORE=1000, Nseq=8, Len=229 C1 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C2 MSTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C3 MSSNSTESVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C4 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C5 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C6 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C7 MMTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C8 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV :.*.::****************************************** C1 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C3 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C4 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C5 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C6 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C7 EVLIMWLLWLLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C8 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII ::******* **************************************** C1 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C3 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C4 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C5 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C6 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C7 NSFKLYRRTRTFWAFNPETDAIAVISVFGRFYSIPMPVAPTGITLTILSG C8 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG ****************************** ******************* C1 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C3 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C4 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C5 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C6 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C7 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C8 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF ************************************************** C1 YVRSKHGDYSALSNSSDNLTENDRLLHLV C2 YVRSKHGDYSALSNSSDNLTENDRLLHLV C3 YVRSKHGDYSALSNSSDNLTENDRLLHLV C4 YVRSKHGDYSALSNSSDNLTENDRLLHLV C5 YVRSKHGDYSALSNSSDNLTENDRLLHLV C6 YVRSKHGDYSALSNSSDNLTENDRLLHLV C7 YVRSKHGDYSALSNSSDNLTENDRLLHLV C8 YVRSKHGDYSALSNSSDNLTENDRLLHLV ***************************** -- Starting log on Fri Oct 21 22:38:45 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.08 sec, SCORE=996, Nseq=8, Len=229 C1 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C2 MSTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C3 MSSNSTESVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C4 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C5 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C6 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C7 MMTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C8 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV :.*.::****************************************** C1 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C3 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C4 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C5 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C6 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C7 EVLIMWLLWLLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C8 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII ::******* **************************************** C1 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C3 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C4 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C5 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C6 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C7 NSFKLYRRTRTFWAFNPETDAIAVISVFGRFYSIPMPVAPTGITLTILSG C8 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG ****************************** ******************* C1 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C3 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C4 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C5 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C6 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C7 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C8 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF ************************************************** C1 YVRSKHGDYSALSNSSDNLTENDRLLHLV C2 YVRSKHGDYSALSNSSDNLTENDRLLHLV C3 YVRSKHGDYSALSNSSDNLTENDRLLHLV C4 YVRSKHGDYSALSNSSDNLTENDRLLHLV C5 YVRSKHGDYSALSNSSDNLTENDRLLHLV C6 YVRSKHGDYSALSNSSDNLTENDRLLHLV C7 YVRSKHGDYSALSNSSDNLTENDRLLHLV C8 YVRSKHGDYSALSNSSDNLTENDRLLHLV ***************************** -- Starting log on Fri Oct 21 22:38:19 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.08 sec, SCORE=1000, Nseq=8, Len=229 C1 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C2 MSTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C3 MSSNSTESVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C4 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C5 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C6 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C7 MMTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV C8 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV :.*.::****************************************** C1 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C3 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C4 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C5 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C6 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C7 EVLIMWLLWLLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII C8 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII ::******* **************************************** C1 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C3 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C4 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C5 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C6 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG C7 NSFKLYRRTRTFWAFNPETDAIAVISVFGRFYSIPMPVAPTGITLTILSG C8 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG ****************************** ******************* C1 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C3 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C4 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C5 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C6 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C7 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF C8 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF ************************************************** C1 YVRSKHGDYSALSNSSDNLTENDRLLHLV C2 YVRSKHGDYSALSNSSDNLTENDRLLHLV C3 YVRSKHGDYSALSNSSDNLTENDRLLHLV C4 YVRSKHGDYSALSNSSDNLTENDRLLHLV C5 YVRSKHGDYSALSNSSDNLTENDRLLHLV C6 YVRSKHGDYSALSNSSDNLTENDRLLHLV C7 YVRSKHGDYSALSNSSDNLTENDRLLHLV C8 YVRSKHGDYSALSNSSDNLTENDRLLHLV ***************************** -- Starting log on Fri Oct 21 22:47:53 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/gapped_alignment/fubar,HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 8 taxa and 687 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C2 Taxon 3 -> C3 Taxon 4 -> C4 Taxon 5 -> C5 Taxon 6 -> C6 Taxon 7 -> C7 Taxon 8 -> C8 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1666392485 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 809758280 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 8650669575 Seed = 517929275 Swapseed = 1666392485 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 11 unique site patterns Division 2 has 9 unique site patterns Division 3 has 16 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -1420.498211 -- 29.153684 Chain 2 -- -1398.178430 -- 29.153684 Chain 3 -- -1402.866443 -- 29.153684 Chain 4 -- -1450.634970 -- 29.153684 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -1454.585342 -- 29.153684 Chain 2 -- -1398.139155 -- 29.153684 Chain 3 -- -1405.137774 -- 29.153684 Chain 4 -- -1457.719132 -- 29.153684 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-1420.498] (-1398.178) (-1402.866) (-1450.635) * [-1454.585] (-1398.139) (-1405.138) (-1457.719) 1000 -- (-1150.740) [-1157.189] (-1151.656) (-1159.324) * (-1160.539) [-1149.871] (-1152.796) (-1149.416) -- 0:00:00 2000 -- (-1156.436) (-1155.586) [-1151.884] (-1151.169) * [-1140.794] (-1147.813) (-1167.827) (-1154.977) -- 0:00:00 3000 -- (-1154.455) (-1155.177) [-1147.806] (-1152.273) * [-1141.188] (-1151.149) (-1154.265) (-1158.464) -- 0:05:32 4000 -- [-1149.496] (-1160.726) (-1145.444) (-1152.102) * [-1140.078] (-1148.928) (-1153.647) (-1155.058) -- 0:04:09 5000 -- (-1152.981) (-1150.936) (-1146.658) [-1145.297] * [-1141.232] (-1146.591) (-1152.899) (-1148.925) -- 0:03:19 Average standard deviation of split frequencies: 0.069838 6000 -- (-1143.370) (-1140.914) [-1138.674] (-1140.262) * [-1143.186] (-1146.017) (-1145.540) (-1151.632) -- 0:02:45 7000 -- (-1139.019) (-1141.175) (-1143.207) [-1141.083] * (-1140.175) (-1140.791) (-1150.074) [-1141.769] -- 0:02:21 8000 -- [-1138.781] (-1146.374) (-1144.061) (-1144.176) * [-1144.482] (-1147.566) (-1142.737) (-1147.228) -- 0:04:08 9000 -- (-1145.049) (-1143.986) [-1139.772] (-1147.186) * (-1142.388) (-1136.020) (-1147.074) [-1146.981] -- 0:03:40 10000 -- (-1143.524) (-1143.333) (-1144.138) [-1136.896] * (-1143.031) (-1147.010) [-1144.317] (-1146.084) -- 0:03:18 Average standard deviation of split frequencies: 0.058926 11000 -- (-1144.670) (-1141.134) (-1143.506) [-1140.470] * [-1140.199] (-1147.752) (-1144.637) (-1145.211) -- 0:02:59 12000 -- (-1148.015) (-1143.016) (-1147.947) [-1141.741] * (-1143.512) (-1149.008) [-1146.637] (-1149.374) -- 0:04:07 13000 -- [-1141.193] (-1139.297) (-1142.254) (-1149.000) * (-1149.504) (-1140.954) (-1141.691) [-1140.021] -- 0:03:47 14000 -- (-1141.273) (-1140.975) [-1141.049] (-1146.126) * (-1145.933) [-1143.499] (-1149.211) (-1142.817) -- 0:03:31 15000 -- (-1144.869) [-1142.313] (-1146.853) (-1143.618) * (-1146.186) [-1139.179] (-1146.107) (-1147.199) -- 0:03:17 Average standard deviation of split frequencies: 0.045831 16000 -- (-1146.245) (-1142.188) (-1144.193) [-1150.401] * (-1143.938) (-1138.704) (-1140.552) [-1144.186] -- 0:03:04 17000 -- (-1141.062) (-1144.029) (-1142.130) [-1142.428] * (-1137.463) (-1139.609) [-1142.313] (-1148.658) -- 0:03:51 18000 -- (-1140.599) (-1141.403) [-1142.524] (-1142.564) * (-1149.254) (-1144.020) [-1147.264] (-1151.182) -- 0:03:38 19000 -- (-1141.750) [-1143.434] (-1142.806) (-1145.042) * (-1150.698) [-1144.697] (-1144.289) (-1139.909) -- 0:03:26 20000 -- (-1149.212) [-1142.164] (-1139.134) (-1142.698) * (-1150.790) [-1144.939] (-1143.961) (-1138.265) -- 0:03:16 Average standard deviation of split frequencies: 0.060826 21000 -- (-1148.220) (-1148.816) [-1135.134] (-1144.657) * (-1151.097) [-1140.766] (-1155.632) (-1148.139) -- 0:03:06 22000 -- (-1147.177) (-1148.575) [-1138.893] (-1145.323) * (-1147.559) (-1140.281) [-1148.207] (-1140.426) -- 0:03:42 23000 -- [-1147.333] (-1143.623) (-1147.579) (-1141.197) * (-1146.236) [-1139.939] (-1148.269) (-1138.874) -- 0:03:32 24000 -- (-1152.777) (-1149.805) (-1142.311) [-1143.585] * (-1146.746) (-1149.313) (-1149.467) [-1146.633] -- 0:03:23 25000 -- [-1138.795] (-1148.243) (-1144.775) (-1141.260) * (-1143.719) (-1144.720) [-1142.236] (-1146.110) -- 0:03:15 Average standard deviation of split frequencies: 0.036262 26000 -- (-1144.398) (-1147.640) [-1139.198] (-1141.706) * (-1142.722) (-1146.025) [-1140.137] (-1139.719) -- 0:03:44 27000 -- (-1141.905) (-1145.335) (-1150.493) [-1149.030] * (-1151.848) (-1143.548) [-1140.412] (-1138.710) -- 0:03:36 28000 -- (-1146.375) (-1147.070) [-1142.912] (-1144.435) * (-1140.092) [-1143.754] (-1143.987) (-1141.018) -- 0:03:28 29000 -- (-1137.983) [-1142.398] (-1146.465) (-1148.432) * [-1141.087] (-1148.095) (-1146.226) (-1143.086) -- 0:03:20 30000 -- (-1147.189) (-1146.210) (-1141.565) [-1146.739] * (-1143.472) (-1148.185) [-1143.291] (-1146.074) -- 0:03:46 Average standard deviation of split frequencies: 0.032452 31000 -- [-1143.375] (-1146.245) (-1149.206) (-1146.139) * [-1143.445] (-1144.951) (-1139.292) (-1142.766) -- 0:03:38 32000 -- (-1140.644) [-1142.966] (-1145.336) (-1144.934) * [-1144.915] (-1149.770) (-1146.044) (-1138.514) -- 0:03:31 33000 -- (-1144.437) [-1145.510] (-1146.271) (-1147.798) * (-1140.607) (-1142.315) [-1144.355] (-1141.357) -- 0:03:25 34000 -- (-1145.594) [-1146.534] (-1141.982) (-1149.152) * (-1142.682) (-1150.943) [-1136.818] (-1144.990) -- 0:03:18 35000 -- (-1149.869) (-1145.516) [-1152.795] (-1143.225) * (-1143.496) (-1140.339) [-1149.475] (-1143.386) -- 0:03:40 Average standard deviation of split frequencies: 0.036374 36000 -- (-1144.599) (-1151.941) [-1143.830] (-1138.518) * (-1142.600) (-1148.431) (-1148.827) [-1140.106] -- 0:03:34 37000 -- [-1139.216] (-1148.266) (-1141.277) (-1155.260) * (-1147.390) (-1139.872) (-1149.950) [-1139.764] -- 0:03:28 38000 -- [-1145.542] (-1149.195) (-1142.308) (-1147.691) * (-1138.674) (-1137.222) [-1144.927] (-1151.767) -- 0:03:22 39000 -- (-1142.752) (-1147.423) (-1139.879) [-1140.628] * [-1147.376] (-1141.629) (-1147.996) (-1145.220) -- 0:03:17 40000 -- (-1139.700) [-1142.578] (-1144.651) (-1143.905) * (-1138.800) (-1141.745) (-1146.093) [-1140.570] -- 0:03:36 Average standard deviation of split frequencies: 0.029624 41000 -- [-1138.383] (-1144.767) (-1137.233) (-1142.463) * (-1147.576) (-1140.840) [-1144.396] (-1146.075) -- 0:03:30 42000 -- [-1141.569] (-1138.526) (-1138.537) (-1143.956) * [-1144.955] (-1133.714) (-1143.151) (-1141.764) -- 0:03:25 43000 -- (-1145.779) [-1139.753] (-1147.546) (-1143.214) * (-1140.348) (-1138.946) [-1142.569] (-1146.157) -- 0:03:20 44000 -- [-1143.246] (-1144.508) (-1143.028) (-1143.001) * (-1141.613) [-1145.205] (-1141.647) (-1141.467) -- 0:03:15 45000 -- (-1145.663) (-1141.300) (-1141.420) [-1145.407] * (-1145.372) (-1140.328) (-1148.510) [-1145.514] -- 0:03:32 Average standard deviation of split frequencies: 0.023912 46000 -- [-1140.049] (-1140.873) (-1144.990) (-1144.826) * (-1139.818) (-1139.409) (-1148.228) [-1145.286] -- 0:03:27 47000 -- (-1140.779) (-1155.109) (-1140.277) [-1146.277] * (-1141.958) (-1136.865) (-1144.102) [-1144.447] -- 0:03:22 48000 -- (-1144.998) (-1153.822) [-1140.758] (-1147.167) * (-1150.212) (-1144.282) (-1145.775) [-1140.140] -- 0:03:18 49000 -- (-1139.919) (-1146.084) [-1140.691] (-1153.898) * [-1139.750] (-1142.313) (-1145.814) (-1145.173) -- 0:03:14 50000 -- (-1137.112) [-1141.513] (-1146.727) (-1144.757) * (-1141.370) (-1144.599) [-1139.639] (-1142.556) -- 0:03:29 Average standard deviation of split frequencies: 0.029980 51000 -- [-1142.553] (-1145.335) (-1147.269) (-1141.549) * (-1145.711) (-1142.057) (-1147.103) [-1141.087] -- 0:03:24 52000 -- [-1143.422] (-1140.651) (-1146.150) (-1145.153) * (-1143.922) (-1142.324) [-1141.394] (-1137.450) -- 0:03:20 53000 -- [-1141.063] (-1141.272) (-1147.297) (-1147.488) * (-1144.445) (-1153.003) (-1135.860) [-1139.488] -- 0:03:16 54000 -- (-1136.651) (-1142.821) [-1141.286] (-1145.033) * (-1145.962) (-1145.160) [-1145.339] (-1145.202) -- 0:03:12 55000 -- (-1145.670) [-1136.645] (-1141.101) (-1147.583) * [-1139.820] (-1149.623) (-1142.110) (-1142.834) -- 0:03:26 Average standard deviation of split frequencies: 0.040219 56000 -- (-1150.430) (-1145.092) [-1145.582] (-1143.907) * [-1150.603] (-1139.188) (-1143.871) (-1138.208) -- 0:03:22 57000 -- (-1141.094) [-1145.012] (-1139.018) (-1144.561) * [-1144.769] (-1147.900) (-1142.697) (-1138.704) -- 0:03:18 58000 -- (-1141.655) (-1137.416) [-1143.935] (-1145.460) * (-1143.540) (-1160.476) [-1142.072] (-1142.092) -- 0:03:14 59000 -- (-1153.093) (-1144.988) [-1141.203] (-1141.326) * (-1139.964) (-1157.517) [-1140.415] (-1144.700) -- 0:03:11 60000 -- (-1144.409) (-1148.051) (-1137.992) [-1136.735] * (-1148.718) (-1154.991) [-1143.478] (-1150.577) -- 0:03:23 Average standard deviation of split frequencies: 0.038852 61000 -- (-1141.384) (-1146.770) (-1138.656) [-1137.439] * (-1143.227) (-1146.393) (-1144.903) [-1139.023] -- 0:03:20 62000 -- (-1148.841) (-1148.280) [-1143.780] (-1139.645) * [-1145.385] (-1142.982) (-1142.558) (-1145.041) -- 0:03:16 63000 -- (-1142.654) (-1154.939) (-1145.176) [-1151.289] * (-1141.430) [-1137.979] (-1146.380) (-1148.087) -- 0:03:13 64000 -- (-1154.015) (-1141.688) [-1140.186] (-1148.614) * [-1146.275] (-1143.980) (-1145.063) (-1147.866) -- 0:03:10 65000 -- (-1141.358) (-1145.496) [-1139.021] (-1142.281) * (-1137.496) [-1144.206] (-1159.581) (-1145.968) -- 0:03:21 Average standard deviation of split frequencies: 0.032538 66000 -- (-1143.544) (-1149.582) [-1139.524] (-1149.000) * [-1141.216] (-1148.824) (-1155.028) (-1145.925) -- 0:03:18 67000 -- [-1139.256] (-1145.093) (-1141.921) (-1146.116) * [-1141.590] (-1153.187) (-1143.046) (-1141.672) -- 0:03:14 68000 -- [-1145.266] (-1150.430) (-1152.667) (-1141.604) * (-1147.685) [-1145.278] (-1151.176) (-1146.362) -- 0:03:11 69000 -- [-1142.126] (-1146.964) (-1146.514) (-1149.436) * [-1149.902] (-1147.183) (-1139.627) (-1145.897) -- 0:03:08 70000 -- (-1140.375) (-1147.935) (-1145.594) [-1146.463] * (-1142.212) (-1151.332) (-1143.908) [-1139.328] -- 0:03:19 Average standard deviation of split frequencies: 0.032613 71000 -- [-1144.947] (-1150.599) (-1150.341) (-1151.012) * (-1149.339) (-1141.032) [-1142.159] (-1140.505) -- 0:03:16 72000 -- (-1145.796) (-1143.798) [-1143.807] (-1150.479) * (-1148.973) (-1143.784) [-1141.628] (-1137.877) -- 0:03:13 73000 -- [-1145.741] (-1148.881) (-1145.033) (-1138.016) * (-1145.165) (-1153.744) [-1145.221] (-1146.803) -- 0:03:10 74000 -- (-1138.557) (-1145.210) [-1143.321] (-1147.480) * [-1148.308] (-1153.089) (-1144.903) (-1150.472) -- 0:03:07 75000 -- [-1138.247] (-1152.622) (-1149.910) (-1141.380) * (-1153.356) [-1149.270] (-1142.804) (-1140.788) -- 0:03:05 Average standard deviation of split frequencies: 0.030324 76000 -- (-1146.442) (-1145.897) [-1135.368] (-1146.369) * (-1148.643) (-1144.775) [-1146.603] (-1145.220) -- 0:03:14 77000 -- (-1144.144) [-1144.284] (-1148.954) (-1149.853) * (-1147.689) (-1143.256) [-1141.072] (-1142.371) -- 0:03:11 78000 -- [-1146.024] (-1147.696) (-1138.181) (-1145.821) * (-1142.411) [-1143.094] (-1144.660) (-1146.772) -- 0:03:09 79000 -- (-1148.953) (-1150.461) [-1139.589] (-1147.147) * (-1146.693) [-1140.660] (-1144.990) (-1143.437) -- 0:03:06 80000 -- [-1148.475] (-1144.044) (-1144.390) (-1145.290) * (-1143.413) (-1144.599) (-1141.842) [-1142.742] -- 0:03:04 Average standard deviation of split frequencies: 0.031167 81000 -- (-1144.036) (-1145.993) (-1144.322) [-1141.366] * (-1142.886) (-1146.106) [-1147.567] (-1136.697) -- 0:03:12 82000 -- [-1142.766] (-1153.237) (-1141.044) (-1146.696) * (-1140.766) (-1146.656) [-1143.474] (-1142.792) -- 0:03:10 83000 -- (-1147.756) (-1143.718) [-1142.031] (-1143.539) * (-1145.543) (-1140.263) [-1139.425] (-1150.884) -- 0:03:07 84000 -- (-1142.003) (-1143.061) [-1149.344] (-1142.883) * (-1141.979) (-1143.555) (-1146.139) [-1145.012] -- 0:03:05 85000 -- [-1148.740] (-1150.862) (-1148.628) (-1144.749) * (-1145.915) (-1145.708) [-1149.924] (-1144.346) -- 0:03:13 Average standard deviation of split frequencies: 0.032280 86000 -- (-1144.997) (-1142.199) (-1144.561) [-1144.710] * (-1145.211) (-1145.910) (-1139.106) [-1138.733] -- 0:03:11 87000 -- [-1139.781] (-1150.490) (-1150.946) (-1138.786) * (-1147.223) (-1141.993) [-1143.315] (-1144.067) -- 0:03:08 88000 -- (-1152.373) [-1151.479] (-1142.829) (-1144.278) * [-1144.391] (-1145.144) (-1140.903) (-1144.917) -- 0:03:06 89000 -- [-1143.118] (-1141.823) (-1147.070) (-1145.019) * [-1139.996] (-1153.195) (-1145.871) (-1146.303) -- 0:03:04 90000 -- (-1143.615) [-1145.013] (-1142.857) (-1141.945) * (-1140.089) (-1147.511) [-1143.639] (-1144.469) -- 0:03:12 Average standard deviation of split frequencies: 0.026574 91000 -- [-1145.927] (-1146.279) (-1150.950) (-1138.959) * (-1136.393) [-1142.336] (-1141.203) (-1144.346) -- 0:03:09 92000 -- [-1143.404] (-1147.640) (-1154.987) (-1145.821) * (-1146.680) (-1143.492) [-1145.807] (-1141.769) -- 0:03:07 93000 -- [-1141.981] (-1140.571) (-1143.814) (-1141.827) * (-1142.924) (-1143.952) [-1139.062] (-1144.127) -- 0:03:05 94000 -- (-1139.308) (-1139.589) (-1146.003) [-1140.993] * (-1146.165) (-1139.010) (-1139.724) [-1141.218] -- 0:03:03 95000 -- [-1145.909] (-1140.489) (-1142.312) (-1140.695) * (-1140.009) (-1148.453) [-1142.310] (-1143.732) -- 0:03:10 Average standard deviation of split frequencies: 0.027826 96000 -- (-1141.636) [-1142.846] (-1141.519) (-1142.347) * (-1141.966) (-1141.272) (-1148.131) [-1141.210] -- 0:03:08 97000 -- [-1141.522] (-1136.522) (-1143.856) (-1146.033) * [-1136.829] (-1149.925) (-1163.525) (-1144.449) -- 0:03:06 98000 -- (-1151.186) (-1148.290) [-1143.468] (-1149.585) * (-1137.494) (-1142.842) (-1148.658) [-1142.977] -- 0:03:04 99000 -- [-1141.780] (-1142.227) (-1150.991) (-1148.016) * (-1143.022) [-1139.903] (-1140.877) (-1144.839) -- 0:03:02 100000 -- (-1141.224) [-1141.436] (-1145.559) (-1147.672) * (-1146.548) (-1144.778) [-1143.080] (-1151.176) -- 0:03:09 Average standard deviation of split frequencies: 0.024455 101000 -- [-1141.378] (-1144.143) (-1145.076) (-1139.520) * (-1138.923) (-1143.670) [-1144.895] (-1144.575) -- 0:03:06 102000 -- (-1143.240) (-1141.877) [-1144.852] (-1149.089) * (-1142.757) (-1146.605) [-1143.344] (-1146.675) -- 0:03:04 103000 -- (-1146.200) (-1149.281) (-1144.516) [-1145.181] * (-1141.212) (-1149.305) (-1142.175) [-1141.097] -- 0:03:02 104000 -- [-1143.202] (-1147.654) (-1143.916) (-1142.260) * (-1143.904) (-1147.170) [-1137.714] (-1147.877) -- 0:03:00 105000 -- (-1145.327) (-1148.157) [-1137.492] (-1152.247) * (-1143.281) [-1146.055] (-1142.935) (-1146.426) -- 0:03:07 Average standard deviation of split frequencies: 0.026189 106000 -- (-1147.186) (-1150.201) (-1144.298) [-1151.127] * (-1141.554) (-1141.330) [-1142.628] (-1141.713) -- 0:03:05 107000 -- [-1141.336] (-1150.573) (-1140.225) (-1156.317) * (-1149.047) (-1141.174) [-1141.791] (-1142.132) -- 0:03:03 108000 -- [-1150.210] (-1150.828) (-1139.524) (-1143.760) * (-1140.625) (-1144.590) [-1139.298] (-1150.558) -- 0:03:01 109000 -- (-1145.885) (-1142.277) (-1152.923) [-1148.754] * [-1141.261] (-1143.649) (-1141.162) (-1147.829) -- 0:02:59 110000 -- (-1136.799) [-1150.745] (-1138.295) (-1147.174) * [-1143.989] (-1141.651) (-1139.344) (-1144.710) -- 0:03:06 Average standard deviation of split frequencies: 0.027925 111000 -- [-1138.581] (-1141.519) (-1146.986) (-1137.882) * [-1142.070] (-1148.790) (-1142.578) (-1144.573) -- 0:03:04 112000 -- (-1144.904) (-1143.115) (-1142.105) [-1140.967] * (-1141.119) (-1143.779) [-1140.255] (-1142.508) -- 0:03:02 113000 -- [-1143.957] (-1142.723) (-1145.375) (-1144.157) * (-1140.562) (-1145.769) (-1149.553) [-1144.824] -- 0:03:00 114000 -- (-1140.179) [-1143.650] (-1143.936) (-1147.925) * (-1142.918) (-1146.759) [-1142.237] (-1148.487) -- 0:02:58 115000 -- [-1140.922] (-1148.662) (-1142.838) (-1148.312) * (-1146.186) [-1141.504] (-1146.728) (-1142.232) -- 0:03:04 Average standard deviation of split frequencies: 0.027544 116000 -- (-1142.334) [-1145.805] (-1144.076) (-1143.114) * (-1147.318) (-1143.229) [-1143.518] (-1139.997) -- 0:03:02 117000 -- [-1140.822] (-1146.659) (-1150.936) (-1151.769) * [-1143.217] (-1143.610) (-1144.663) (-1151.004) -- 0:03:01 118000 -- (-1148.092) (-1150.858) (-1147.335) [-1143.833] * (-1151.152) (-1145.288) [-1137.443] (-1150.987) -- 0:02:59 119000 -- (-1147.743) (-1145.127) [-1147.851] (-1142.185) * (-1143.315) [-1138.729] (-1145.168) (-1142.964) -- 0:02:57 120000 -- (-1151.854) (-1141.507) [-1144.163] (-1146.037) * [-1146.469] (-1143.523) (-1142.819) (-1142.493) -- 0:03:03 Average standard deviation of split frequencies: 0.023874 121000 -- (-1145.132) (-1148.823) [-1139.664] (-1144.201) * (-1148.004) (-1140.155) [-1137.692] (-1139.627) -- 0:03:01 122000 -- (-1146.422) (-1146.936) (-1148.363) [-1140.708] * [-1149.282] (-1141.335) (-1142.783) (-1150.727) -- 0:02:59 123000 -- (-1147.752) (-1148.135) [-1143.838] (-1138.266) * (-1146.079) [-1144.829] (-1145.572) (-1143.299) -- 0:02:58 124000 -- (-1142.811) (-1144.248) (-1144.854) [-1141.264] * (-1144.790) [-1142.542] (-1141.565) (-1145.577) -- 0:02:56 125000 -- [-1144.675] (-1147.650) (-1147.596) (-1136.992) * (-1143.657) [-1137.679] (-1144.158) (-1141.665) -- 0:03:02 Average standard deviation of split frequencies: 0.021201 126000 -- (-1145.514) (-1141.479) (-1139.113) [-1143.691] * (-1142.374) (-1144.379) (-1146.217) [-1139.617] -- 0:03:00 127000 -- (-1146.135) (-1142.556) (-1138.904) [-1144.042] * (-1138.133) (-1149.521) (-1144.453) [-1148.935] -- 0:02:58 128000 -- [-1142.476] (-1142.754) (-1145.052) (-1138.643) * (-1147.042) [-1146.697] (-1144.278) (-1150.415) -- 0:02:57 129000 -- (-1150.192) (-1149.663) (-1143.015) [-1139.136] * [-1143.793] (-1143.400) (-1146.161) (-1141.230) -- 0:02:55 130000 -- (-1145.452) (-1147.439) (-1145.890) [-1141.965] * (-1139.875) [-1148.793] (-1149.816) (-1144.884) -- 0:03:00 Average standard deviation of split frequencies: 0.022849 131000 -- (-1145.820) (-1143.119) (-1143.221) [-1144.542] * [-1142.318] (-1145.354) (-1144.163) (-1145.098) -- 0:02:59 132000 -- [-1146.019] (-1148.656) (-1150.594) (-1139.978) * (-1143.973) (-1144.367) [-1144.379] (-1139.263) -- 0:02:57 133000 -- (-1149.425) [-1142.402] (-1143.585) (-1155.082) * (-1143.284) (-1144.523) (-1153.113) [-1141.286] -- 0:02:56 134000 -- (-1149.954) [-1142.423] (-1147.356) (-1143.587) * (-1151.659) (-1152.058) (-1150.733) [-1137.823] -- 0:02:54 135000 -- (-1152.086) (-1144.544) (-1137.249) [-1143.992] * (-1149.105) [-1147.233] (-1145.820) (-1139.694) -- 0:02:59 Average standard deviation of split frequencies: 0.023493 136000 -- [-1141.334] (-1145.253) (-1139.880) (-1148.754) * [-1142.562] (-1147.512) (-1143.721) (-1153.101) -- 0:02:57 137000 -- (-1138.856) (-1144.911) (-1143.726) [-1143.502] * (-1142.272) (-1145.338) [-1141.477] (-1140.184) -- 0:02:56 138000 -- (-1145.868) [-1145.497] (-1144.804) (-1139.587) * (-1141.291) (-1142.006) (-1143.463) [-1144.618] -- 0:02:54 139000 -- [-1138.612] (-1145.355) (-1146.281) (-1136.357) * (-1143.150) (-1141.433) [-1143.972] (-1150.850) -- 0:02:53 140000 -- [-1142.357] (-1152.478) (-1155.485) (-1142.607) * (-1141.065) (-1145.738) [-1146.754] (-1147.582) -- 0:02:58 Average standard deviation of split frequencies: 0.024203 141000 -- [-1146.101] (-1143.581) (-1146.693) (-1137.384) * (-1138.232) [-1143.825] (-1141.497) (-1149.741) -- 0:02:56 142000 -- (-1142.584) (-1149.184) [-1140.855] (-1143.215) * [-1145.370] (-1146.804) (-1141.178) (-1147.725) -- 0:02:55 143000 -- [-1144.582] (-1145.261) (-1143.529) (-1146.489) * [-1139.465] (-1142.538) (-1152.223) (-1145.044) -- 0:02:53 144000 -- (-1140.484) (-1144.797) [-1142.220] (-1139.813) * (-1145.421) (-1144.682) [-1148.645] (-1145.149) -- 0:02:52 145000 -- (-1143.785) (-1140.894) [-1142.297] (-1155.611) * (-1142.733) (-1143.649) (-1148.184) [-1145.991] -- 0:02:56 Average standard deviation of split frequencies: 0.024037 146000 -- [-1139.327] (-1144.305) (-1149.769) (-1144.155) * (-1143.355) (-1139.131) [-1144.572] (-1148.845) -- 0:02:55 147000 -- [-1143.979] (-1145.277) (-1142.244) (-1137.233) * (-1147.694) [-1144.073] (-1141.948) (-1141.228) -- 0:02:54 148000 -- (-1136.955) (-1150.493) [-1141.130] (-1144.368) * (-1144.041) (-1149.362) (-1143.613) [-1137.543] -- 0:02:52 149000 -- (-1141.637) [-1146.307] (-1148.791) (-1142.348) * [-1144.476] (-1151.755) (-1148.992) (-1139.391) -- 0:02:51 150000 -- [-1138.427] (-1144.687) (-1136.269) (-1142.819) * (-1152.523) (-1146.553) (-1144.421) [-1141.390] -- 0:02:55 Average standard deviation of split frequencies: 0.023292 151000 -- (-1138.778) (-1141.694) (-1143.299) [-1137.500] * (-1145.474) (-1151.052) (-1154.270) [-1146.430] -- 0:02:54 152000 -- (-1142.742) (-1141.228) (-1139.255) [-1139.782] * (-1154.917) (-1146.018) (-1158.165) [-1148.350] -- 0:02:52 153000 -- (-1145.177) (-1146.077) [-1138.974] (-1139.611) * (-1150.827) (-1142.456) (-1138.946) [-1144.641] -- 0:02:51 154000 -- [-1140.302] (-1145.487) (-1152.392) (-1143.053) * (-1150.951) (-1143.766) [-1143.217] (-1140.226) -- 0:02:50 155000 -- (-1143.396) (-1142.411) [-1141.154] (-1146.239) * [-1144.718] (-1140.916) (-1143.485) (-1141.994) -- 0:02:54 Average standard deviation of split frequencies: 0.021153 156000 -- (-1148.002) (-1148.762) [-1138.526] (-1148.872) * (-1147.589) (-1139.819) [-1143.943] (-1145.550) -- 0:02:53 157000 -- (-1140.482) (-1143.943) [-1140.995] (-1147.489) * [-1142.921] (-1140.010) (-1150.583) (-1144.715) -- 0:02:51 158000 -- (-1144.193) [-1138.490] (-1142.520) (-1147.323) * (-1141.793) (-1141.792) (-1144.177) [-1145.980] -- 0:02:50 159000 -- [-1150.931] (-1142.574) (-1139.876) (-1147.551) * (-1147.780) [-1143.460] (-1146.962) (-1140.160) -- 0:02:49 160000 -- (-1141.463) (-1141.346) [-1144.323] (-1145.614) * (-1146.221) (-1147.901) [-1142.131] (-1136.800) -- 0:02:53 Average standard deviation of split frequencies: 0.021190 161000 -- (-1140.766) (-1142.917) (-1141.359) [-1148.241] * (-1148.209) [-1142.210] (-1153.809) (-1148.221) -- 0:02:51 162000 -- (-1146.770) [-1145.457] (-1144.726) (-1138.563) * (-1142.450) [-1136.277] (-1147.487) (-1144.250) -- 0:02:50 163000 -- (-1149.246) (-1145.365) [-1138.699] (-1140.011) * [-1144.494] (-1138.994) (-1146.764) (-1148.471) -- 0:02:49 164000 -- (-1149.670) (-1154.092) [-1140.143] (-1141.074) * (-1141.987) (-1150.819) [-1144.104] (-1147.122) -- 0:02:48 165000 -- (-1141.514) (-1142.797) [-1144.189] (-1147.144) * (-1149.324) (-1150.324) (-1149.373) [-1141.593] -- 0:02:52 Average standard deviation of split frequencies: 0.023349 166000 -- (-1142.679) (-1148.263) (-1144.275) [-1142.536] * (-1141.691) [-1139.855] (-1142.572) (-1138.651) -- 0:02:50 167000 -- [-1143.778] (-1145.626) (-1149.454) (-1137.314) * (-1139.641) (-1145.093) [-1143.275] (-1144.149) -- 0:02:49 168000 -- (-1146.479) (-1147.158) [-1142.495] (-1141.094) * (-1143.523) (-1143.145) (-1147.472) [-1146.933] -- 0:02:48 169000 -- (-1154.202) (-1141.604) [-1141.307] (-1150.340) * (-1140.864) [-1139.812] (-1144.753) (-1141.367) -- 0:02:47 170000 -- [-1149.715] (-1149.594) (-1143.665) (-1143.900) * (-1143.837) (-1145.204) (-1144.447) [-1136.976] -- 0:02:50 Average standard deviation of split frequencies: 0.025473 171000 -- (-1141.134) [-1144.168] (-1144.081) (-1137.652) * (-1143.219) (-1143.027) (-1153.375) [-1142.148] -- 0:02:49 172000 -- (-1142.114) (-1142.295) (-1154.417) [-1140.405] * [-1140.723] (-1142.645) (-1142.125) (-1150.558) -- 0:02:48 173000 -- (-1146.041) (-1144.982) [-1139.617] (-1143.803) * (-1149.427) (-1143.936) (-1140.902) [-1142.328] -- 0:02:47 174000 -- (-1141.184) [-1143.922] (-1143.376) (-1145.283) * (-1145.134) (-1141.426) (-1145.394) [-1144.064] -- 0:02:46 175000 -- (-1156.919) (-1139.429) (-1144.129) [-1138.949] * (-1137.879) [-1146.271] (-1145.574) (-1144.394) -- 0:02:49 Average standard deviation of split frequencies: 0.022915 176000 -- (-1146.942) [-1142.704] (-1143.049) (-1144.725) * (-1145.340) (-1146.009) (-1144.570) [-1144.814] -- 0:02:48 177000 -- (-1146.545) (-1141.231) (-1144.176) [-1138.607] * [-1140.154] (-1141.364) (-1156.682) (-1141.809) -- 0:02:47 178000 -- (-1146.720) (-1145.015) [-1139.841] (-1147.577) * (-1144.911) (-1146.636) [-1151.046] (-1147.083) -- 0:02:46 179000 -- [-1139.541] (-1141.435) (-1140.551) (-1145.692) * [-1143.037] (-1145.517) (-1142.427) (-1141.919) -- 0:02:49 180000 -- (-1145.392) (-1148.100) [-1140.004] (-1150.763) * (-1142.806) (-1145.569) (-1145.751) [-1141.606] -- 0:02:48 Average standard deviation of split frequencies: 0.019135 181000 -- (-1142.062) [-1140.458] (-1140.447) (-1147.470) * (-1142.404) (-1147.262) (-1139.798) [-1144.558] -- 0:02:47 182000 -- (-1152.420) [-1139.466] (-1144.995) (-1145.910) * (-1147.331) (-1139.772) [-1141.833] (-1153.455) -- 0:02:46 183000 -- (-1139.883) (-1143.555) [-1144.296] (-1143.865) * (-1141.504) [-1140.512] (-1142.890) (-1152.076) -- 0:02:45 184000 -- (-1147.823) [-1140.145] (-1144.891) (-1144.955) * (-1142.762) [-1141.755] (-1146.215) (-1150.446) -- 0:02:48 185000 -- (-1151.969) [-1143.262] (-1138.086) (-1144.005) * [-1142.729] (-1138.021) (-1146.881) (-1151.967) -- 0:02:47 Average standard deviation of split frequencies: 0.018304 186000 -- (-1144.053) (-1141.035) [-1146.943] (-1152.958) * (-1140.821) [-1141.667] (-1143.801) (-1157.779) -- 0:02:46 187000 -- [-1140.148] (-1150.146) (-1142.039) (-1140.418) * (-1142.717) (-1141.757) [-1143.381] (-1156.238) -- 0:02:45 188000 -- (-1144.412) (-1145.369) (-1137.988) [-1145.893] * (-1148.741) [-1140.983] (-1142.553) (-1146.476) -- 0:02:44 189000 -- (-1142.884) [-1143.259] (-1140.974) (-1143.592) * (-1141.432) (-1143.572) [-1140.648] (-1149.547) -- 0:02:43 190000 -- [-1139.471] (-1152.048) (-1140.618) (-1141.899) * (-1144.259) [-1149.038] (-1143.206) (-1148.977) -- 0:02:46 Average standard deviation of split frequencies: 0.016483 191000 -- [-1149.339] (-1144.578) (-1145.659) (-1140.596) * (-1149.166) [-1141.829] (-1142.653) (-1144.389) -- 0:02:45 192000 -- (-1141.585) [-1141.569] (-1137.670) (-1152.612) * (-1143.656) (-1140.371) (-1139.707) [-1141.911] -- 0:02:44 193000 -- (-1140.838) (-1140.940) [-1143.488] (-1146.148) * (-1149.918) (-1139.535) [-1137.393] (-1152.416) -- 0:02:43 194000 -- (-1140.157) [-1139.086] (-1143.413) (-1141.102) * (-1142.264) (-1140.637) (-1158.162) [-1145.177] -- 0:02:42 195000 -- [-1137.040] (-1139.031) (-1145.808) (-1139.930) * (-1139.612) [-1141.622] (-1143.070) (-1152.107) -- 0:02:45 Average standard deviation of split frequencies: 0.013896 196000 -- (-1141.300) [-1147.360] (-1145.117) (-1153.543) * (-1145.553) (-1145.332) (-1150.802) [-1140.772] -- 0:02:44 197000 -- (-1144.172) (-1143.462) [-1143.419] (-1147.483) * [-1143.202] (-1147.556) (-1141.650) (-1146.005) -- 0:02:43 198000 -- (-1147.047) [-1148.847] (-1143.800) (-1142.685) * (-1148.980) (-1140.938) (-1148.036) [-1137.495] -- 0:02:42 199000 -- (-1139.228) (-1140.408) (-1147.941) [-1145.173] * (-1149.231) (-1141.926) (-1144.864) [-1141.629] -- 0:02:41 200000 -- [-1138.753] (-1140.338) (-1148.587) (-1144.994) * (-1145.815) [-1150.288] (-1152.841) (-1141.023) -- 0:02:44 Average standard deviation of split frequencies: 0.015139 201000 -- (-1143.800) (-1143.361) (-1146.454) [-1141.998] * (-1147.468) (-1145.018) (-1141.487) [-1140.799] -- 0:02:42 202000 -- (-1140.747) (-1138.691) [-1141.513] (-1142.471) * [-1144.613] (-1142.316) (-1141.857) (-1150.630) -- 0:02:41 203000 -- (-1146.649) (-1145.672) (-1153.391) [-1143.864] * (-1140.847) (-1142.307) [-1145.282] (-1148.103) -- 0:02:40 204000 -- (-1144.264) [-1137.999] (-1141.632) (-1140.105) * (-1144.421) (-1150.267) [-1138.732] (-1152.427) -- 0:02:39 205000 -- (-1145.773) (-1138.243) [-1149.286] (-1145.971) * (-1146.560) (-1147.372) [-1141.699] (-1142.208) -- 0:02:42 Average standard deviation of split frequencies: 0.016273 206000 -- (-1155.512) (-1142.765) (-1147.292) [-1146.536] * (-1142.880) (-1149.452) [-1145.811] (-1143.809) -- 0:02:41 207000 -- (-1146.870) [-1141.516] (-1144.764) (-1143.114) * (-1141.799) [-1143.835] (-1145.031) (-1143.269) -- 0:02:40 208000 -- [-1141.367] (-1140.270) (-1141.145) (-1145.878) * (-1147.013) (-1143.647) [-1145.385] (-1144.370) -- 0:02:39 209000 -- [-1143.853] (-1148.711) (-1148.596) (-1141.870) * (-1145.766) [-1141.795] (-1151.705) (-1150.518) -- 0:02:38 210000 -- [-1139.171] (-1141.018) (-1146.873) (-1139.984) * (-1153.885) [-1148.813] (-1145.109) (-1146.089) -- 0:02:41 Average standard deviation of split frequencies: 0.018896 211000 -- (-1144.221) (-1137.942) (-1147.198) [-1138.622] * (-1151.690) [-1146.711] (-1140.252) (-1153.605) -- 0:02:40 212000 -- (-1144.636) (-1149.448) (-1150.403) [-1145.597] * (-1137.201) [-1137.629] (-1156.030) (-1151.213) -- 0:02:39 213000 -- [-1140.035] (-1151.724) (-1149.294) (-1139.973) * (-1150.521) (-1140.070) [-1145.914] (-1146.418) -- 0:02:38 214000 -- (-1143.327) (-1146.797) (-1143.860) [-1146.143] * [-1143.894] (-1144.345) (-1143.750) (-1150.135) -- 0:02:37 215000 -- (-1151.775) (-1146.766) (-1151.345) [-1142.765] * (-1141.365) (-1136.087) [-1143.224] (-1145.895) -- 0:02:40 Average standard deviation of split frequencies: 0.021097 216000 -- (-1149.889) (-1153.215) (-1142.819) [-1139.619] * (-1147.788) [-1140.584] (-1142.899) (-1143.772) -- 0:02:39 217000 -- (-1143.673) (-1152.232) [-1136.741] (-1140.350) * (-1141.392) (-1142.302) (-1150.015) [-1141.081] -- 0:02:38 218000 -- (-1145.740) [-1145.948] (-1143.525) (-1151.724) * [-1149.861] (-1139.389) (-1149.662) (-1143.393) -- 0:02:37 219000 -- [-1143.006] (-1141.208) (-1144.175) (-1149.246) * (-1143.701) (-1145.740) (-1147.275) [-1135.886] -- 0:02:36 220000 -- (-1149.924) (-1148.228) (-1136.443) [-1149.999] * [-1137.248] (-1146.607) (-1148.200) (-1143.589) -- 0:02:39 Average standard deviation of split frequencies: 0.022312 221000 -- (-1143.437) [-1140.267] (-1139.343) (-1141.957) * (-1140.083) [-1143.682] (-1145.328) (-1146.830) -- 0:02:38 222000 -- (-1141.965) [-1147.442] (-1149.984) (-1146.949) * (-1140.279) [-1137.910] (-1149.054) (-1144.321) -- 0:02:37 223000 -- (-1147.350) [-1137.866] (-1149.501) (-1145.892) * [-1144.887] (-1142.001) (-1152.177) (-1137.389) -- 0:02:36 224000 -- (-1142.153) [-1140.881] (-1143.400) (-1144.940) * (-1147.428) [-1136.167] (-1144.704) (-1139.471) -- 0:02:35 225000 -- (-1142.220) [-1141.008] (-1141.925) (-1143.109) * (-1135.298) (-1141.909) (-1144.530) [-1140.103] -- 0:02:38 Average standard deviation of split frequencies: 0.021554 226000 -- [-1139.947] (-1138.504) (-1149.242) (-1141.538) * (-1144.611) (-1142.453) [-1141.762] (-1145.575) -- 0:02:37 227000 -- (-1148.028) (-1144.700) (-1147.466) [-1148.599] * (-1144.258) [-1141.563] (-1141.215) (-1149.232) -- 0:02:36 228000 -- (-1146.774) [-1141.682] (-1147.111) (-1142.364) * (-1143.373) (-1146.461) [-1142.953] (-1143.360) -- 0:02:35 229000 -- (-1153.012) (-1143.168) [-1139.714] (-1150.860) * (-1146.618) [-1139.206] (-1138.839) (-1141.391) -- 0:02:34 230000 -- (-1145.241) [-1145.559] (-1143.499) (-1147.368) * (-1143.783) [-1141.409] (-1143.668) (-1145.014) -- 0:02:37 Average standard deviation of split frequencies: 0.022480 231000 -- (-1147.981) (-1146.351) [-1144.298] (-1139.253) * (-1146.970) [-1139.527] (-1137.360) (-1138.589) -- 0:02:36 232000 -- (-1141.288) (-1142.405) [-1138.331] (-1150.870) * (-1140.020) [-1141.380] (-1140.297) (-1139.471) -- 0:02:35 233000 -- (-1151.479) (-1142.874) [-1140.228] (-1140.685) * (-1143.074) (-1147.958) [-1142.396] (-1142.166) -- 0:02:34 234000 -- [-1143.525] (-1146.403) (-1143.110) (-1142.074) * [-1143.314] (-1147.438) (-1146.966) (-1148.792) -- 0:02:33 235000 -- [-1136.700] (-1144.605) (-1143.281) (-1139.366) * (-1144.717) (-1140.547) (-1144.119) [-1143.912] -- 0:02:36 Average standard deviation of split frequencies: 0.023970 236000 -- [-1144.737] (-1137.164) (-1140.389) (-1148.938) * [-1141.959] (-1141.743) (-1141.986) (-1149.132) -- 0:02:35 237000 -- (-1143.934) (-1141.796) [-1142.582] (-1144.765) * (-1145.307) (-1141.915) (-1143.941) [-1149.011] -- 0:02:34 238000 -- [-1145.239] (-1147.992) (-1146.648) (-1142.724) * (-1150.436) [-1140.467] (-1151.171) (-1152.208) -- 0:02:33 239000 -- (-1145.099) [-1143.789] (-1150.910) (-1144.191) * [-1143.654] (-1146.695) (-1142.081) (-1147.418) -- 0:02:32 240000 -- [-1143.137] (-1142.722) (-1148.110) (-1144.234) * [-1145.590] (-1145.707) (-1143.887) (-1148.855) -- 0:02:35 Average standard deviation of split frequencies: 0.020676 241000 -- (-1145.701) (-1147.717) (-1141.559) [-1139.937] * (-1149.777) (-1137.229) [-1140.956] (-1145.290) -- 0:02:34 242000 -- (-1146.704) (-1148.042) (-1143.754) [-1140.776] * [-1142.495] (-1138.789) (-1141.695) (-1142.129) -- 0:02:33 243000 -- (-1146.460) (-1147.053) (-1148.709) [-1140.721] * (-1140.195) [-1140.106] (-1141.788) (-1141.714) -- 0:02:32 244000 -- [-1148.377] (-1152.556) (-1145.255) (-1144.631) * (-1152.328) (-1145.889) [-1143.356] (-1141.351) -- 0:02:31 245000 -- [-1143.386] (-1150.521) (-1140.072) (-1137.158) * (-1140.266) (-1143.727) (-1154.156) [-1144.662] -- 0:02:31 Average standard deviation of split frequencies: 0.022144 246000 -- (-1143.952) (-1152.482) [-1143.443] (-1141.185) * (-1147.934) (-1145.071) (-1141.552) [-1143.398] -- 0:02:33 247000 -- (-1140.110) (-1146.002) [-1140.567] (-1139.036) * [-1142.564] (-1142.672) (-1140.171) (-1139.063) -- 0:02:32 248000 -- (-1150.862) [-1140.124] (-1141.552) (-1140.058) * [-1141.708] (-1148.414) (-1141.396) (-1147.498) -- 0:02:31 249000 -- [-1143.228] (-1143.921) (-1137.097) (-1146.268) * (-1146.982) (-1157.178) (-1142.907) [-1139.314] -- 0:02:30 250000 -- (-1148.203) (-1139.377) (-1141.286) [-1144.823] * (-1149.242) (-1144.682) [-1142.178] (-1147.971) -- 0:02:30 Average standard deviation of split frequencies: 0.022149 251000 -- (-1145.616) [-1136.209] (-1149.420) (-1143.796) * (-1145.448) (-1142.043) [-1142.355] (-1147.163) -- 0:02:32 252000 -- (-1141.925) (-1146.689) (-1147.589) [-1151.868] * (-1142.913) (-1148.548) [-1137.720] (-1142.427) -- 0:02:31 253000 -- [-1141.782] (-1147.869) (-1143.058) (-1149.363) * [-1145.032] (-1149.201) (-1142.410) (-1145.654) -- 0:02:30 254000 -- (-1144.518) (-1143.808) [-1143.115] (-1154.348) * [-1145.621] (-1145.796) (-1140.677) (-1157.921) -- 0:02:29 255000 -- (-1141.060) (-1136.831) [-1140.613] (-1146.698) * (-1149.670) [-1144.594] (-1139.112) (-1143.605) -- 0:02:29 Average standard deviation of split frequencies: 0.021688 256000 -- [-1138.475] (-1148.852) (-1142.802) (-1139.652) * [-1144.736] (-1142.093) (-1137.113) (-1155.786) -- 0:02:31 257000 -- (-1144.465) (-1148.114) [-1147.605] (-1139.869) * (-1152.176) (-1147.914) (-1143.086) [-1137.105] -- 0:02:30 258000 -- (-1141.040) (-1139.731) [-1136.425] (-1147.142) * (-1143.119) (-1140.708) [-1137.027] (-1141.682) -- 0:02:29 259000 -- (-1144.308) (-1141.442) (-1146.229) [-1138.458] * (-1145.789) [-1144.152] (-1143.916) (-1145.117) -- 0:02:28 260000 -- (-1148.211) (-1143.144) [-1141.628] (-1138.892) * (-1144.976) [-1146.646] (-1136.009) (-1143.429) -- 0:02:28 Average standard deviation of split frequencies: 0.020496 261000 -- (-1149.112) (-1153.181) (-1140.869) [-1140.760] * [-1146.106] (-1144.025) (-1146.067) (-1144.210) -- 0:02:30 262000 -- [-1143.474] (-1147.222) (-1146.978) (-1143.264) * (-1146.016) [-1149.601] (-1145.457) (-1140.097) -- 0:02:29 263000 -- [-1142.701] (-1157.542) (-1145.159) (-1143.381) * (-1150.150) [-1142.934] (-1142.804) (-1147.965) -- 0:02:28 264000 -- [-1146.873] (-1152.445) (-1142.445) (-1137.036) * (-1152.059) (-1142.145) (-1144.380) [-1137.073] -- 0:02:27 265000 -- (-1140.542) (-1152.468) (-1152.545) [-1141.473] * [-1141.817] (-1145.312) (-1146.533) (-1143.043) -- 0:02:27 Average standard deviation of split frequencies: 0.018313 266000 -- (-1141.270) (-1143.427) (-1146.482) [-1151.599] * [-1144.532] (-1148.989) (-1143.498) (-1144.148) -- 0:02:29 267000 -- (-1140.152) (-1146.792) (-1142.999) [-1142.519] * (-1141.441) [-1139.266] (-1138.176) (-1143.223) -- 0:02:28 268000 -- (-1146.477) (-1147.870) (-1146.245) [-1145.416] * (-1146.543) [-1144.292] (-1150.689) (-1143.860) -- 0:02:27 269000 -- (-1146.113) [-1145.494] (-1140.432) (-1146.961) * (-1148.859) (-1155.674) (-1144.778) [-1141.269] -- 0:02:26 270000 -- (-1146.248) (-1151.905) (-1142.730) [-1137.968] * (-1142.711) (-1143.084) (-1144.367) [-1143.715] -- 0:02:26 Average standard deviation of split frequencies: 0.017610 271000 -- (-1141.729) (-1143.322) [-1151.366] (-1142.949) * (-1141.879) (-1143.395) (-1153.933) [-1141.455] -- 0:02:27 272000 -- (-1140.520) (-1143.250) (-1145.006) [-1142.231] * (-1147.369) (-1151.210) [-1142.289] (-1140.738) -- 0:02:27 273000 -- [-1138.993] (-1142.996) (-1139.169) (-1148.490) * [-1142.716] (-1155.391) (-1143.034) (-1146.687) -- 0:02:26 274000 -- (-1148.037) (-1146.094) (-1146.601) [-1138.930] * (-1143.040) (-1152.791) [-1140.082] (-1139.454) -- 0:02:25 275000 -- (-1141.053) (-1145.979) [-1147.229] (-1148.616) * [-1141.494] (-1147.994) (-1135.620) (-1144.913) -- 0:02:25 Average standard deviation of split frequencies: 0.017649 276000 -- (-1146.000) (-1143.830) [-1141.040] (-1139.260) * [-1139.413] (-1150.997) (-1151.432) (-1144.588) -- 0:02:26 277000 -- (-1145.784) [-1145.870] (-1150.186) (-1145.691) * (-1142.350) (-1145.642) [-1141.262] (-1144.544) -- 0:02:26 278000 -- (-1140.169) [-1148.441] (-1148.384) (-1139.360) * (-1139.848) (-1138.614) (-1146.735) [-1146.246] -- 0:02:25 279000 -- (-1147.340) (-1145.588) (-1144.107) [-1143.489] * (-1140.608) [-1142.075] (-1143.920) (-1148.259) -- 0:02:24 280000 -- (-1151.949) (-1138.366) (-1145.751) [-1144.199] * [-1143.157] (-1147.062) (-1142.218) (-1141.321) -- 0:02:24 Average standard deviation of split frequencies: 0.016983 281000 -- (-1155.615) (-1149.869) [-1140.339] (-1143.525) * (-1142.766) [-1138.093] (-1143.594) (-1153.143) -- 0:02:25 282000 -- (-1144.494) [-1151.630] (-1146.362) (-1146.540) * [-1144.483] (-1143.622) (-1150.622) (-1150.587) -- 0:02:25 283000 -- (-1141.840) (-1141.009) [-1143.981] (-1140.192) * (-1140.928) [-1139.661] (-1151.323) (-1143.824) -- 0:02:24 284000 -- (-1143.345) (-1144.626) [-1152.398] (-1142.761) * (-1150.045) (-1141.881) [-1150.294] (-1143.586) -- 0:02:23 285000 -- (-1144.674) (-1141.684) (-1154.834) [-1146.574] * (-1146.799) [-1143.232] (-1156.556) (-1140.150) -- 0:02:23 Average standard deviation of split frequencies: 0.017582 286000 -- (-1141.170) [-1143.151] (-1149.602) (-1140.167) * (-1144.363) (-1140.809) (-1147.848) [-1147.318] -- 0:02:24 287000 -- (-1146.651) (-1145.396) (-1153.023) [-1141.522] * (-1150.189) (-1139.683) (-1146.872) [-1140.655] -- 0:02:24 288000 -- (-1135.128) (-1145.282) (-1145.820) [-1146.677] * [-1143.873] (-1147.467) (-1145.688) (-1147.383) -- 0:02:23 289000 -- [-1142.904] (-1151.760) (-1146.626) (-1145.213) * (-1143.728) (-1150.749) (-1148.540) [-1138.756] -- 0:02:22 290000 -- (-1146.966) (-1147.271) [-1143.270] (-1150.350) * [-1141.783] (-1147.310) (-1140.870) (-1150.907) -- 0:02:22 Average standard deviation of split frequencies: 0.015858 291000 -- [-1141.255] (-1157.376) (-1148.109) (-1140.632) * (-1144.151) (-1154.546) (-1146.165) [-1144.511] -- 0:02:23 292000 -- (-1145.315) (-1151.229) (-1150.352) [-1144.632] * [-1141.479] (-1153.823) (-1146.802) (-1143.674) -- 0:02:23 293000 -- [-1139.574] (-1149.925) (-1147.128) (-1145.140) * [-1137.851] (-1147.155) (-1146.087) (-1144.826) -- 0:02:22 294000 -- (-1150.858) (-1148.715) (-1146.830) [-1138.105] * (-1146.507) (-1144.595) (-1155.094) [-1147.215] -- 0:02:21 295000 -- (-1138.039) [-1144.264] (-1146.280) (-1144.232) * (-1145.259) (-1146.709) (-1140.146) [-1142.337] -- 0:02:21 Average standard deviation of split frequencies: 0.016457 296000 -- [-1141.385] (-1147.175) (-1146.479) (-1144.549) * (-1146.670) (-1144.291) (-1137.908) [-1145.735] -- 0:02:22 297000 -- (-1141.631) [-1148.023] (-1144.656) (-1141.532) * (-1145.526) (-1140.927) (-1142.898) [-1139.845] -- 0:02:22 298000 -- (-1139.761) [-1141.490] (-1149.436) (-1144.202) * [-1146.363] (-1148.311) (-1150.653) (-1148.476) -- 0:02:21 299000 -- (-1150.292) (-1144.288) (-1143.447) [-1149.482] * (-1146.701) (-1142.703) [-1143.400] (-1144.716) -- 0:02:20 300000 -- [-1142.282] (-1138.131) (-1145.700) (-1146.723) * (-1147.972) [-1143.975] (-1143.063) (-1142.299) -- 0:02:20 Average standard deviation of split frequencies: 0.015504 301000 -- (-1144.096) [-1145.232] (-1146.927) (-1143.528) * [-1146.144] (-1149.745) (-1139.025) (-1141.093) -- 0:02:21 302000 -- (-1142.355) (-1151.069) [-1141.091] (-1142.614) * (-1144.932) [-1150.067] (-1147.552) (-1139.877) -- 0:02:20 303000 -- (-1142.983) (-1153.556) (-1142.079) [-1144.743] * [-1141.538] (-1145.429) (-1140.536) (-1149.101) -- 0:02:20 304000 -- (-1142.941) (-1147.277) (-1152.460) [-1145.150] * [-1146.370] (-1138.587) (-1141.291) (-1143.729) -- 0:02:19 305000 -- [-1136.577] (-1145.632) (-1142.620) (-1144.386) * (-1137.706) (-1150.503) (-1142.547) [-1136.857] -- 0:02:19 Average standard deviation of split frequencies: 0.014207 306000 -- (-1145.112) (-1153.443) [-1142.338] (-1140.730) * (-1141.869) [-1151.472] (-1148.252) (-1146.106) -- 0:02:20 307000 -- (-1141.884) (-1152.150) (-1142.025) [-1142.983] * [-1146.738] (-1144.509) (-1149.592) (-1143.749) -- 0:02:19 308000 -- [-1139.846] (-1148.552) (-1139.707) (-1139.212) * (-1143.016) [-1142.566] (-1151.442) (-1146.843) -- 0:02:19 309000 -- [-1148.159] (-1146.778) (-1145.888) (-1145.899) * (-1144.577) (-1142.386) [-1147.664] (-1149.714) -- 0:02:18 310000 -- [-1141.393] (-1142.145) (-1142.741) (-1140.689) * (-1137.230) (-1145.540) (-1148.469) [-1147.202] -- 0:02:18 Average standard deviation of split frequencies: 0.013825 311000 -- (-1149.739) (-1141.713) [-1140.721] (-1142.728) * [-1137.880] (-1140.564) (-1149.193) (-1145.521) -- 0:02:17 312000 -- [-1140.827] (-1154.869) (-1148.080) (-1144.163) * (-1144.359) (-1138.962) [-1140.623] (-1141.046) -- 0:02:18 313000 -- (-1142.621) [-1142.076] (-1144.248) (-1140.995) * (-1146.849) (-1142.744) [-1141.786] (-1149.150) -- 0:02:18 314000 -- [-1138.441] (-1144.478) (-1144.789) (-1146.462) * (-1144.329) [-1139.718] (-1150.280) (-1140.676) -- 0:02:17 315000 -- (-1152.474) [-1146.402] (-1137.898) (-1139.226) * (-1145.123) (-1141.367) [-1146.524] (-1148.388) -- 0:02:17 Average standard deviation of split frequencies: 0.013923 316000 -- [-1143.096] (-1147.898) (-1142.199) (-1140.639) * [-1143.581] (-1139.907) (-1145.289) (-1152.356) -- 0:02:18 317000 -- [-1141.872] (-1144.445) (-1144.168) (-1145.145) * (-1142.436) [-1145.534] (-1145.269) (-1144.445) -- 0:02:17 318000 -- (-1147.288) (-1140.168) (-1145.005) [-1143.061] * (-1140.801) [-1143.473] (-1141.567) (-1145.236) -- 0:02:17 319000 -- [-1143.335] (-1144.084) (-1143.819) (-1149.042) * (-1142.654) (-1142.315) (-1138.912) [-1145.836] -- 0:02:16 320000 -- (-1154.825) (-1140.788) (-1144.466) [-1139.181] * (-1141.792) [-1145.122] (-1144.769) (-1142.641) -- 0:02:16 Average standard deviation of split frequencies: 0.012904 321000 -- (-1145.624) (-1141.283) (-1143.354) [-1140.294] * [-1142.729] (-1145.605) (-1141.378) (-1142.425) -- 0:02:15 322000 -- (-1152.750) [-1140.245] (-1148.264) (-1144.129) * (-1155.190) (-1143.886) (-1147.405) [-1144.640] -- 0:02:16 323000 -- (-1148.187) (-1144.287) (-1148.056) [-1144.746] * (-1145.062) (-1142.762) (-1140.161) [-1142.067] -- 0:02:16 324000 -- (-1153.795) (-1139.110) [-1148.819] (-1141.641) * [-1141.954] (-1145.445) (-1137.332) (-1142.194) -- 0:02:15 325000 -- (-1149.677) [-1148.622] (-1151.546) (-1142.545) * [-1148.821] (-1144.822) (-1152.089) (-1138.880) -- 0:02:15 Average standard deviation of split frequencies: 0.013336 326000 -- (-1149.069) [-1142.934] (-1146.321) (-1143.893) * [-1141.223] (-1140.482) (-1141.785) (-1141.722) -- 0:02:16 327000 -- (-1140.481) (-1142.805) (-1153.541) [-1140.049] * (-1154.513) [-1146.025] (-1146.733) (-1143.327) -- 0:02:15 328000 -- (-1144.280) (-1143.146) [-1138.465] (-1141.675) * [-1137.588] (-1144.531) (-1142.105) (-1146.386) -- 0:02:15 329000 -- (-1147.656) (-1147.817) [-1146.248] (-1145.281) * (-1135.492) (-1142.734) (-1145.125) [-1140.965] -- 0:02:14 330000 -- (-1144.474) (-1153.006) [-1145.892] (-1144.037) * [-1138.529] (-1149.536) (-1139.983) (-1141.780) -- 0:02:14 Average standard deviation of split frequencies: 0.012197 331000 -- (-1148.519) (-1158.316) (-1146.195) [-1138.993] * (-1142.685) (-1150.393) (-1143.621) [-1144.195] -- 0:02:15 332000 -- (-1144.732) (-1146.138) [-1136.458] (-1144.210) * (-1144.015) (-1144.583) [-1140.107] (-1145.248) -- 0:02:14 333000 -- (-1139.431) (-1146.613) (-1144.820) [-1140.320] * (-1139.928) [-1141.945] (-1141.637) (-1146.865) -- 0:02:14 334000 -- [-1141.421] (-1140.977) (-1147.790) (-1141.466) * (-1151.018) [-1145.469] (-1151.662) (-1148.765) -- 0:02:13 335000 -- (-1144.313) (-1147.312) [-1144.509] (-1138.450) * (-1139.350) (-1141.930) (-1144.934) [-1141.502] -- 0:02:13 Average standard deviation of split frequencies: 0.011068 336000 -- (-1144.903) [-1145.594] (-1138.432) (-1144.838) * (-1138.704) [-1145.488] (-1141.474) (-1153.345) -- 0:02:12 337000 -- (-1143.211) [-1139.176] (-1142.861) (-1155.350) * (-1145.379) (-1144.586) [-1140.824] (-1145.191) -- 0:02:13 338000 -- (-1148.130) (-1144.172) [-1140.640] (-1150.506) * (-1144.583) (-1137.927) (-1142.268) [-1139.727] -- 0:02:13 339000 -- (-1138.610) (-1146.335) [-1139.263] (-1150.044) * (-1142.375) (-1141.122) [-1142.727] (-1152.055) -- 0:02:12 340000 -- (-1146.718) [-1155.432] (-1139.460) (-1142.972) * (-1143.507) (-1149.745) (-1141.775) [-1139.153] -- 0:02:12 Average standard deviation of split frequencies: 0.011685 341000 -- (-1143.008) (-1148.506) [-1138.877] (-1149.744) * (-1141.536) (-1145.148) [-1139.152] (-1147.509) -- 0:02:11 342000 -- [-1140.722] (-1143.114) (-1137.661) (-1148.725) * (-1144.948) (-1140.899) (-1151.821) [-1143.199] -- 0:02:12 343000 -- [-1140.690] (-1153.317) (-1142.416) (-1141.752) * (-1141.903) (-1146.656) [-1141.004] (-1139.130) -- 0:02:12 344000 -- (-1156.499) [-1141.647] (-1148.792) (-1139.180) * (-1138.823) (-1147.960) (-1150.636) [-1138.718] -- 0:02:11 345000 -- (-1141.716) (-1147.172) [-1141.098] (-1147.368) * [-1142.832] (-1141.076) (-1142.417) (-1150.743) -- 0:02:11 Average standard deviation of split frequencies: 0.010900 346000 -- (-1143.919) [-1144.502] (-1146.460) (-1143.957) * (-1135.687) (-1150.476) (-1143.427) [-1141.868] -- 0:02:12 347000 -- [-1141.057] (-1147.068) (-1144.039) (-1141.188) * (-1146.864) [-1141.764] (-1144.857) (-1144.064) -- 0:02:11 348000 -- [-1146.457] (-1143.187) (-1147.909) (-1142.637) * (-1142.771) (-1145.309) (-1144.727) [-1137.431] -- 0:02:11 349000 -- (-1141.785) (-1145.113) [-1144.261] (-1151.766) * (-1145.799) (-1142.675) [-1138.050] (-1150.670) -- 0:02:10 350000 -- [-1141.367] (-1140.605) (-1150.782) (-1145.816) * [-1140.608] (-1145.017) (-1140.867) (-1140.077) -- 0:02:10 Average standard deviation of split frequencies: 0.009410 351000 -- (-1141.453) [-1142.167] (-1142.907) (-1143.623) * (-1141.838) [-1143.625] (-1145.716) (-1150.078) -- 0:02:11 352000 -- [-1144.893] (-1151.571) (-1148.742) (-1158.931) * (-1143.603) (-1150.219) (-1145.038) [-1146.240] -- 0:02:10 353000 -- [-1138.709] (-1149.069) (-1141.500) (-1142.584) * [-1143.114] (-1142.851) (-1147.825) (-1140.762) -- 0:02:10 354000 -- (-1142.214) (-1143.621) [-1146.401] (-1142.873) * (-1141.936) [-1141.319] (-1145.464) (-1140.705) -- 0:02:09 355000 -- (-1162.009) [-1141.935] (-1143.744) (-1144.047) * (-1142.382) (-1145.470) (-1142.933) [-1138.180] -- 0:02:09 Average standard deviation of split frequencies: 0.012212 356000 -- (-1149.381) (-1143.809) [-1136.999] (-1141.333) * [-1138.448] (-1143.294) (-1148.045) (-1144.778) -- 0:02:10 357000 -- [-1141.500] (-1141.728) (-1142.136) (-1139.680) * (-1140.602) (-1138.434) (-1145.837) [-1139.680] -- 0:02:09 358000 -- (-1145.105) (-1149.704) [-1145.891] (-1137.422) * (-1142.256) (-1136.958) [-1142.123] (-1146.488) -- 0:02:09 359000 -- (-1143.156) (-1142.148) (-1140.181) [-1141.767] * (-1139.200) (-1140.500) [-1141.727] (-1142.816) -- 0:02:08 360000 -- [-1142.096] (-1149.509) (-1143.905) (-1137.453) * (-1141.501) (-1146.045) [-1147.390] (-1143.550) -- 0:02:08 Average standard deviation of split frequencies: 0.010892 361000 -- (-1148.979) [-1138.632] (-1147.549) (-1138.305) * (-1145.361) (-1147.379) (-1147.105) [-1139.207] -- 0:02:09 362000 -- (-1150.795) (-1140.483) (-1155.108) [-1140.459] * (-1142.263) [-1146.010] (-1147.986) (-1144.286) -- 0:02:08 363000 -- (-1146.839) (-1142.522) (-1140.794) [-1142.886] * (-1141.836) (-1141.988) [-1144.754] (-1141.639) -- 0:02:08 364000 -- (-1143.117) (-1153.098) [-1147.048] (-1142.666) * [-1144.551] (-1143.191) (-1149.324) (-1144.343) -- 0:02:07 365000 -- (-1142.659) (-1144.970) [-1137.999] (-1143.766) * (-1145.621) (-1141.370) (-1145.256) [-1139.565] -- 0:02:07 Average standard deviation of split frequencies: 0.010447 366000 -- (-1146.329) [-1143.964] (-1147.781) (-1153.848) * (-1143.028) (-1144.500) (-1146.602) [-1142.120] -- 0:02:06 367000 -- (-1146.282) [-1138.237] (-1144.226) (-1139.373) * (-1147.228) (-1141.936) [-1143.491] (-1150.877) -- 0:02:07 368000 -- (-1146.955) [-1138.191] (-1151.409) (-1139.073) * (-1146.746) (-1139.927) [-1140.370] (-1143.176) -- 0:02:07 369000 -- (-1146.846) (-1141.147) [-1135.330] (-1144.352) * (-1141.785) (-1147.975) (-1149.907) [-1145.201] -- 0:02:06 370000 -- [-1139.485] (-1152.108) (-1145.234) (-1147.401) * (-1144.541) [-1141.873] (-1147.910) (-1142.743) -- 0:02:06 Average standard deviation of split frequencies: 0.012294 371000 -- [-1144.177] (-1149.795) (-1137.341) (-1138.031) * [-1139.947] (-1140.341) (-1152.584) (-1140.867) -- 0:02:05 372000 -- (-1147.390) [-1145.221] (-1148.590) (-1141.133) * (-1151.136) (-1137.859) (-1144.094) [-1136.567] -- 0:02:06 373000 -- (-1140.232) [-1138.873] (-1147.876) (-1148.132) * (-1144.625) (-1148.988) (-1155.729) [-1145.163] -- 0:02:06 374000 -- [-1146.538] (-1140.247) (-1153.468) (-1147.509) * (-1146.289) (-1142.923) (-1148.847) [-1139.432] -- 0:02:05 375000 -- (-1148.361) (-1144.841) (-1149.613) [-1147.765] * (-1144.687) (-1141.445) [-1141.977] (-1146.254) -- 0:02:05 Average standard deviation of split frequencies: 0.012677 376000 -- (-1144.093) [-1139.196] (-1141.653) (-1146.259) * (-1145.213) [-1139.284] (-1145.776) (-1146.840) -- 0:02:04 377000 -- (-1143.041) (-1143.854) (-1147.248) [-1141.115] * (-1146.903) [-1142.582] (-1142.658) (-1144.484) -- 0:02:05 378000 -- [-1144.289] (-1139.378) (-1141.396) (-1141.312) * (-1149.210) (-1143.348) (-1146.972) [-1142.514] -- 0:02:05 379000 -- [-1138.438] (-1143.539) (-1149.068) (-1147.967) * (-1142.912) (-1145.380) (-1140.545) [-1142.641] -- 0:02:04 380000 -- (-1144.934) [-1140.684] (-1146.321) (-1149.515) * (-1144.215) (-1154.603) (-1144.913) [-1142.266] -- 0:02:04 Average standard deviation of split frequencies: 0.014035 381000 -- (-1153.843) (-1144.139) (-1147.712) [-1148.550] * (-1148.546) (-1146.600) [-1144.297] (-1144.328) -- 0:02:03 382000 -- (-1144.293) (-1146.405) (-1149.216) [-1142.773] * (-1148.713) [-1143.819] (-1144.882) (-1146.578) -- 0:02:04 383000 -- (-1148.995) [-1143.813] (-1147.902) (-1139.442) * [-1143.043] (-1141.243) (-1150.335) (-1150.339) -- 0:02:04 384000 -- (-1141.057) [-1140.200] (-1146.329) (-1137.317) * [-1140.943] (-1139.267) (-1152.929) (-1143.121) -- 0:02:03 385000 -- (-1146.221) [-1142.501] (-1147.029) (-1142.670) * (-1144.895) [-1140.004] (-1151.565) (-1149.753) -- 0:02:03 Average standard deviation of split frequencies: 0.014112 386000 -- (-1144.917) [-1138.939] (-1150.622) (-1151.527) * (-1140.861) (-1146.552) [-1141.845] (-1147.048) -- 0:02:02 387000 -- (-1145.747) (-1142.369) [-1153.122] (-1142.166) * (-1143.465) (-1143.910) [-1140.240] (-1145.824) -- 0:02:03 388000 -- (-1153.695) (-1154.856) (-1148.203) [-1140.455] * (-1142.893) (-1147.085) (-1150.012) [-1147.425] -- 0:02:03 389000 -- (-1142.212) (-1145.312) (-1152.914) [-1138.128] * [-1139.846] (-1145.888) (-1149.957) (-1143.839) -- 0:02:02 390000 -- [-1142.031] (-1141.790) (-1153.366) (-1141.247) * (-1137.810) [-1144.712] (-1146.533) (-1142.716) -- 0:02:02 Average standard deviation of split frequencies: 0.014078 391000 -- [-1148.648] (-1146.886) (-1150.933) (-1142.887) * [-1142.183] (-1145.918) (-1148.347) (-1141.335) -- 0:02:01 392000 -- [-1145.625] (-1145.377) (-1161.182) (-1147.751) * (-1138.389) [-1143.266] (-1146.082) (-1143.589) -- 0:02:02 393000 -- (-1140.908) [-1143.639] (-1156.037) (-1145.324) * (-1140.987) (-1144.713) (-1146.489) [-1146.515] -- 0:02:02 394000 -- [-1144.493] (-1143.268) (-1151.069) (-1143.878) * (-1145.908) [-1144.335] (-1140.898) (-1143.087) -- 0:02:01 395000 -- (-1150.538) (-1146.102) (-1148.154) [-1149.974] * (-1144.292) (-1143.254) (-1145.563) [-1145.954] -- 0:02:01 Average standard deviation of split frequencies: 0.014682 396000 -- [-1141.817] (-1141.843) (-1148.047) (-1139.902) * (-1147.439) (-1151.441) (-1145.067) [-1141.534] -- 0:02:00 397000 -- (-1155.813) [-1145.887] (-1154.178) (-1141.661) * (-1141.507) (-1141.954) (-1147.908) [-1141.123] -- 0:02:01 398000 -- (-1150.405) [-1140.770] (-1147.353) (-1139.578) * [-1140.580] (-1148.756) (-1140.937) (-1140.979) -- 0:02:01 399000 -- (-1143.513) [-1142.135] (-1143.244) (-1141.295) * [-1144.199] (-1156.687) (-1143.135) (-1145.681) -- 0:02:00 400000 -- [-1136.887] (-1141.777) (-1145.610) (-1147.116) * [-1141.949] (-1143.751) (-1154.000) (-1136.686) -- 0:02:00 Average standard deviation of split frequencies: 0.015295 401000 -- (-1142.088) [-1146.717] (-1146.440) (-1141.197) * (-1149.184) (-1145.515) (-1142.660) [-1151.030] -- 0:01:59 402000 -- (-1141.902) [-1141.527] (-1145.524) (-1142.276) * [-1137.073] (-1145.755) (-1143.348) (-1149.586) -- 0:02:00 403000 -- (-1140.861) [-1141.727] (-1146.304) (-1149.279) * (-1145.968) (-1144.829) [-1146.249] (-1142.356) -- 0:01:59 404000 -- [-1140.192] (-1149.573) (-1140.269) (-1151.025) * (-1139.147) (-1141.305) (-1149.721) [-1144.692] -- 0:01:59 405000 -- (-1144.426) (-1148.569) [-1134.279] (-1147.014) * (-1147.442) [-1148.400] (-1137.111) (-1146.514) -- 0:01:59 Average standard deviation of split frequencies: 0.015997 406000 -- (-1142.310) [-1141.771] (-1154.497) (-1145.958) * [-1142.064] (-1144.386) (-1139.571) (-1148.339) -- 0:01:58 407000 -- (-1144.004) [-1140.839] (-1146.201) (-1147.957) * (-1147.131) (-1143.639) (-1142.453) [-1145.043] -- 0:01:58 408000 -- (-1146.567) [-1138.264] (-1138.555) (-1149.584) * (-1146.661) (-1149.576) (-1141.400) [-1141.050] -- 0:01:58 409000 -- [-1143.145] (-1146.752) (-1146.186) (-1143.086) * (-1142.643) (-1145.862) [-1138.188] (-1146.873) -- 0:01:58 410000 -- (-1138.965) [-1140.807] (-1145.564) (-1147.619) * (-1142.376) [-1143.832] (-1151.322) (-1148.632) -- 0:01:58 Average standard deviation of split frequencies: 0.017091 411000 -- [-1144.084] (-1147.139) (-1143.314) (-1147.218) * (-1142.412) (-1144.779) [-1140.256] (-1150.431) -- 0:01:57 412000 -- (-1147.896) (-1148.256) (-1142.185) [-1145.999] * (-1144.748) [-1143.771] (-1145.073) (-1144.076) -- 0:01:57 413000 -- (-1148.159) (-1140.141) (-1139.043) [-1144.607] * (-1141.523) [-1145.229] (-1143.528) (-1143.036) -- 0:01:57 414000 -- (-1143.913) (-1142.456) (-1139.110) [-1155.471] * (-1152.914) [-1143.910] (-1150.262) (-1140.049) -- 0:01:57 415000 -- (-1146.914) (-1143.157) [-1141.466] (-1143.003) * (-1145.413) [-1139.613] (-1157.590) (-1147.871) -- 0:01:57 Average standard deviation of split frequencies: 0.015235 416000 -- (-1142.238) (-1143.580) [-1139.240] (-1146.730) * [-1141.157] (-1143.963) (-1148.228) (-1144.395) -- 0:01:56 417000 -- (-1142.534) (-1138.077) (-1142.290) [-1146.331] * (-1140.554) [-1142.279] (-1144.667) (-1146.033) -- 0:01:56 418000 -- (-1139.251) (-1143.170) (-1154.609) [-1141.654] * (-1137.573) [-1143.094] (-1145.346) (-1145.492) -- 0:01:56 419000 -- (-1140.670) [-1142.698] (-1145.991) (-1143.805) * [-1143.687] (-1141.591) (-1150.065) (-1146.188) -- 0:01:56 420000 -- [-1137.044] (-1138.080) (-1145.576) (-1141.895) * (-1149.125) (-1147.218) (-1140.809) [-1153.648] -- 0:01:56 Average standard deviation of split frequencies: 0.017058 421000 -- [-1136.404] (-1146.313) (-1144.995) (-1141.866) * (-1143.390) [-1139.082] (-1139.718) (-1139.754) -- 0:01:55 422000 -- (-1148.783) (-1149.008) (-1146.087) [-1138.449] * (-1145.097) [-1142.103] (-1149.946) (-1142.639) -- 0:01:55 423000 -- (-1145.879) (-1143.517) [-1138.334] (-1139.310) * (-1139.988) (-1144.495) (-1149.343) [-1139.436] -- 0:01:55 424000 -- (-1150.137) (-1151.064) (-1144.525) [-1145.851] * (-1146.108) (-1145.735) (-1139.223) [-1136.673] -- 0:01:55 425000 -- (-1141.911) [-1147.356] (-1144.597) (-1150.375) * (-1143.839) [-1142.754] (-1140.488) (-1140.497) -- 0:01:55 Average standard deviation of split frequencies: 0.017828 426000 -- [-1144.683] (-1152.169) (-1149.463) (-1143.562) * (-1142.130) (-1141.369) (-1140.310) [-1151.694] -- 0:01:54 427000 -- (-1147.128) (-1146.785) [-1136.606] (-1137.291) * (-1147.302) (-1145.165) [-1137.734] (-1155.320) -- 0:01:54 428000 -- [-1151.434] (-1146.203) (-1136.646) (-1141.575) * (-1138.029) [-1137.678] (-1147.561) (-1151.525) -- 0:01:54 429000 -- (-1145.520) [-1143.345] (-1148.112) (-1140.422) * (-1138.579) [-1144.784] (-1139.024) (-1146.927) -- 0:01:54 430000 -- [-1142.620] (-1150.780) (-1145.147) (-1141.294) * (-1144.001) [-1139.789] (-1148.067) (-1141.741) -- 0:01:54 Average standard deviation of split frequencies: 0.019216 431000 -- (-1149.281) (-1142.677) (-1142.494) [-1146.118] * (-1140.357) (-1145.691) [-1145.066] (-1143.330) -- 0:01:53 432000 -- [-1145.982] (-1142.135) (-1140.422) (-1158.609) * (-1146.734) (-1146.414) (-1153.132) [-1143.387] -- 0:01:53 433000 -- (-1142.842) (-1142.655) [-1137.572] (-1146.537) * (-1147.379) (-1143.846) (-1146.737) [-1140.500] -- 0:01:53 434000 -- (-1142.228) (-1143.039) (-1144.818) [-1141.139] * (-1143.267) (-1139.158) [-1144.739] (-1142.750) -- 0:01:53 435000 -- (-1139.241) (-1138.405) (-1150.213) [-1142.656] * (-1143.166) [-1144.446] (-1145.362) (-1135.269) -- 0:01:53 Average standard deviation of split frequencies: 0.019462 436000 -- (-1139.611) (-1150.521) (-1152.816) [-1147.474] * (-1142.831) (-1142.210) (-1143.433) [-1145.161] -- 0:01:52 437000 -- (-1146.257) [-1147.482] (-1147.933) (-1147.540) * (-1143.249) (-1141.450) [-1139.039] (-1150.864) -- 0:01:52 438000 -- (-1140.183) (-1148.834) (-1147.436) [-1145.073] * (-1145.443) (-1151.530) [-1143.147] (-1140.642) -- 0:01:52 439000 -- (-1144.593) [-1145.806] (-1141.954) (-1144.062) * (-1143.284) (-1151.398) [-1143.631] (-1154.422) -- 0:01:52 440000 -- [-1143.436] (-1148.936) (-1142.004) (-1156.064) * (-1145.798) (-1146.198) (-1145.793) [-1145.343] -- 0:01:52 Average standard deviation of split frequencies: 0.019850 441000 -- [-1142.789] (-1140.098) (-1149.073) (-1146.690) * [-1142.404] (-1146.633) (-1146.662) (-1146.575) -- 0:01:51 442000 -- (-1144.083) (-1153.281) (-1144.475) [-1142.517] * [-1140.002] (-1149.252) (-1144.030) (-1140.478) -- 0:01:51 443000 -- [-1142.397] (-1141.850) (-1138.780) (-1142.453) * [-1144.397] (-1149.274) (-1145.005) (-1142.298) -- 0:01:51 444000 -- [-1141.082] (-1148.162) (-1147.930) (-1144.376) * (-1144.827) [-1145.253] (-1153.145) (-1140.911) -- 0:01:51 445000 -- (-1142.514) (-1139.702) [-1145.795] (-1140.567) * (-1147.081) [-1140.676] (-1138.601) (-1143.172) -- 0:01:51 Average standard deviation of split frequencies: 0.020669 446000 -- (-1147.257) (-1143.726) [-1146.426] (-1140.340) * [-1148.742] (-1148.025) (-1145.635) (-1143.751) -- 0:01:50 447000 -- (-1140.321) [-1144.106] (-1153.713) (-1143.751) * (-1151.363) [-1145.363] (-1144.641) (-1145.017) -- 0:01:50 448000 -- [-1148.541] (-1143.094) (-1150.874) (-1152.415) * (-1148.020) [-1141.097] (-1149.852) (-1140.486) -- 0:01:50 449000 -- (-1146.498) (-1150.812) [-1145.469] (-1142.941) * [-1141.117] (-1147.430) (-1148.660) (-1144.971) -- 0:01:50 450000 -- (-1139.739) (-1149.173) (-1145.241) [-1142.942] * (-1143.798) [-1147.907] (-1149.207) (-1142.711) -- 0:01:50 Average standard deviation of split frequencies: 0.018828 451000 -- (-1152.820) (-1142.749) (-1158.217) [-1146.013] * (-1142.254) [-1143.699] (-1143.565) (-1144.606) -- 0:01:49 452000 -- (-1142.487) (-1145.369) (-1150.875) [-1139.910] * (-1149.203) (-1141.366) [-1139.517] (-1146.909) -- 0:01:49 453000 -- (-1142.020) [-1141.058] (-1146.204) (-1148.167) * (-1142.856) (-1144.096) (-1138.605) [-1139.218] -- 0:01:49 454000 -- (-1142.975) [-1148.629] (-1149.590) (-1145.915) * (-1148.063) (-1146.699) [-1142.000] (-1138.293) -- 0:01:49 455000 -- (-1143.823) [-1152.667] (-1149.279) (-1144.196) * (-1144.984) (-1138.409) (-1146.816) [-1141.257] -- 0:01:49 Average standard deviation of split frequencies: 0.020216 456000 -- [-1137.681] (-1150.049) (-1149.299) (-1139.938) * [-1140.150] (-1148.051) (-1150.064) (-1147.719) -- 0:01:48 457000 -- (-1149.088) [-1145.542] (-1147.440) (-1148.534) * (-1143.977) (-1145.864) (-1147.376) [-1145.865] -- 0:01:48 458000 -- (-1145.109) (-1143.629) [-1143.157] (-1138.119) * [-1144.120] (-1146.730) (-1144.077) (-1137.912) -- 0:01:48 459000 -- (-1156.677) (-1139.230) (-1151.225) [-1141.123] * (-1143.300) (-1140.223) (-1145.515) [-1141.794] -- 0:01:48 460000 -- (-1145.493) (-1146.523) [-1140.256] (-1140.025) * (-1144.963) (-1141.136) (-1140.959) [-1145.429] -- 0:01:48 Average standard deviation of split frequencies: 0.019557 461000 -- (-1145.218) [-1143.635] (-1142.951) (-1145.279) * (-1136.135) (-1143.986) [-1148.118] (-1149.445) -- 0:01:47 462000 -- (-1142.706) [-1141.662] (-1138.962) (-1140.840) * (-1144.087) [-1144.519] (-1148.092) (-1153.179) -- 0:01:47 463000 -- (-1146.200) (-1141.225) (-1149.377) [-1137.853] * (-1145.687) [-1145.192] (-1138.983) (-1147.122) -- 0:01:46 464000 -- (-1143.657) (-1145.355) (-1145.989) [-1144.739] * [-1141.046] (-1142.872) (-1142.549) (-1145.200) -- 0:01:47 465000 -- (-1139.690) [-1139.837] (-1149.933) (-1144.630) * (-1138.273) [-1141.491] (-1145.094) (-1145.594) -- 0:01:47 Average standard deviation of split frequencies: 0.018883 466000 -- [-1148.457] (-1138.074) (-1149.485) (-1143.412) * (-1142.521) [-1145.441] (-1141.786) (-1146.502) -- 0:01:46 467000 -- (-1144.637) (-1147.386) [-1142.382] (-1148.982) * (-1139.017) (-1149.835) [-1141.788] (-1142.536) -- 0:01:46 468000 -- (-1141.802) (-1141.656) [-1147.803] (-1156.337) * (-1137.006) (-1143.833) (-1143.337) [-1141.908] -- 0:01:45 469000 -- [-1142.601] (-1142.315) (-1148.129) (-1145.462) * (-1140.051) [-1144.737] (-1139.533) (-1149.953) -- 0:01:46 470000 -- (-1141.518) (-1139.464) [-1142.604] (-1142.480) * (-1141.841) [-1144.735] (-1144.344) (-1143.032) -- 0:01:46 Average standard deviation of split frequencies: 0.019141 471000 -- (-1145.113) (-1144.857) [-1142.240] (-1141.848) * (-1144.846) (-1145.065) [-1143.113] (-1153.031) -- 0:01:45 472000 -- (-1151.502) (-1147.660) (-1143.823) [-1145.149] * (-1142.259) (-1147.397) [-1147.579] (-1145.895) -- 0:01:45 473000 -- (-1148.181) [-1139.156] (-1143.766) (-1150.418) * (-1146.695) (-1147.941) [-1141.629] (-1149.027) -- 0:01:44 474000 -- (-1142.080) [-1138.623] (-1143.341) (-1145.491) * (-1143.210) (-1145.386) [-1136.642] (-1151.021) -- 0:01:45 475000 -- [-1142.783] (-1147.144) (-1144.306) (-1143.394) * (-1144.494) (-1146.122) (-1145.867) [-1143.110] -- 0:01:45 Average standard deviation of split frequencies: 0.019807 476000 -- (-1147.291) [-1136.945] (-1143.458) (-1148.193) * (-1140.575) [-1137.535] (-1146.256) (-1145.375) -- 0:01:44 477000 -- (-1151.047) (-1143.115) (-1139.280) [-1142.778] * [-1140.513] (-1142.756) (-1142.522) (-1144.959) -- 0:01:44 478000 -- (-1145.713) (-1138.373) (-1142.831) [-1136.712] * (-1137.865) [-1139.071] (-1143.333) (-1145.940) -- 0:01:43 479000 -- (-1141.942) [-1141.821] (-1142.788) (-1138.102) * (-1142.391) [-1141.870] (-1142.477) (-1137.538) -- 0:01:44 480000 -- (-1144.949) (-1139.839) (-1143.496) [-1141.724] * [-1142.201] (-1140.845) (-1147.404) (-1141.906) -- 0:01:44 Average standard deviation of split frequencies: 0.018961 481000 -- (-1145.464) (-1148.559) [-1140.063] (-1140.597) * [-1137.944] (-1146.812) (-1151.425) (-1139.950) -- 0:01:43 482000 -- (-1151.086) (-1146.663) [-1141.664] (-1144.383) * [-1146.802] (-1145.928) (-1153.107) (-1145.586) -- 0:01:43 483000 -- [-1143.108] (-1146.008) (-1141.417) (-1147.837) * [-1142.638] (-1141.148) (-1145.956) (-1147.655) -- 0:01:42 484000 -- (-1142.250) (-1142.000) [-1150.832] (-1140.196) * [-1142.645] (-1147.627) (-1144.538) (-1154.512) -- 0:01:43 485000 -- (-1141.946) (-1141.349) [-1142.086] (-1142.361) * (-1148.799) (-1139.554) [-1138.138] (-1148.222) -- 0:01:43 Average standard deviation of split frequencies: 0.019076 486000 -- (-1146.463) [-1142.807] (-1144.099) (-1149.546) * [-1141.309] (-1146.096) (-1142.768) (-1161.188) -- 0:01:42 487000 -- [-1141.122] (-1139.342) (-1151.550) (-1147.263) * (-1147.784) (-1145.031) (-1145.255) [-1142.629] -- 0:01:42 488000 -- (-1144.007) [-1143.586] (-1143.266) (-1141.759) * (-1139.748) (-1148.663) (-1140.806) [-1141.645] -- 0:01:41 489000 -- (-1139.374) (-1147.613) (-1145.529) [-1137.432] * (-1139.860) [-1140.550] (-1139.674) (-1146.128) -- 0:01:42 490000 -- (-1140.231) (-1145.473) [-1140.906] (-1141.791) * [-1145.075] (-1144.029) (-1143.623) (-1147.630) -- 0:01:42 Average standard deviation of split frequencies: 0.018254 491000 -- (-1142.246) (-1150.328) [-1140.207] (-1143.006) * [-1141.538] (-1145.132) (-1138.208) (-1143.378) -- 0:01:41 492000 -- (-1146.059) (-1142.915) (-1144.403) [-1142.371] * (-1143.561) [-1141.897] (-1141.436) (-1142.047) -- 0:01:41 493000 -- (-1141.472) (-1150.807) [-1138.274] (-1143.652) * [-1143.435] (-1146.678) (-1149.220) (-1141.835) -- 0:01:40 494000 -- (-1147.343) (-1141.767) (-1146.647) [-1140.907] * [-1146.483] (-1146.398) (-1142.338) (-1138.755) -- 0:01:41 495000 -- (-1145.051) [-1146.180] (-1142.696) (-1140.290) * (-1150.747) [-1142.049] (-1142.246) (-1142.105) -- 0:01:41 Average standard deviation of split frequencies: 0.018480 496000 -- (-1147.001) [-1138.290] (-1140.855) (-1144.111) * (-1150.263) (-1142.865) (-1140.341) [-1145.718] -- 0:01:40 497000 -- (-1139.392) (-1150.963) (-1149.908) [-1140.469] * (-1145.047) (-1147.863) [-1142.409] (-1140.520) -- 0:01:40 498000 -- (-1138.547) [-1142.930] (-1139.553) (-1145.941) * [-1145.860] (-1147.907) (-1139.407) (-1145.363) -- 0:01:39 499000 -- (-1145.959) (-1148.135) [-1145.575] (-1144.423) * [-1140.914] (-1143.987) (-1141.893) (-1150.060) -- 0:01:39 500000 -- (-1150.820) (-1142.070) [-1139.747] (-1151.122) * (-1140.827) (-1144.359) [-1143.421] (-1141.374) -- 0:01:40 Average standard deviation of split frequencies: 0.018308 501000 -- [-1145.724] (-1152.422) (-1145.975) (-1141.150) * [-1147.739] (-1137.945) (-1140.598) (-1143.393) -- 0:01:39 502000 -- (-1150.020) (-1144.787) (-1147.579) [-1144.346] * (-1138.646) [-1145.374] (-1144.636) (-1152.453) -- 0:01:39 503000 -- (-1149.278) (-1141.441) (-1145.644) [-1139.812] * (-1141.675) (-1146.111) [-1142.088] (-1148.350) -- 0:01:38 504000 -- [-1139.292] (-1145.660) (-1149.402) (-1147.203) * (-1148.637) (-1141.118) [-1140.184] (-1145.232) -- 0:01:38 505000 -- [-1137.939] (-1144.811) (-1142.575) (-1145.439) * (-1148.079) (-1144.192) (-1142.164) [-1144.860] -- 0:01:39 Average standard deviation of split frequencies: 0.017494 506000 -- (-1143.922) (-1140.119) (-1144.744) [-1138.281] * (-1148.673) [-1140.983] (-1142.148) (-1147.397) -- 0:01:38 507000 -- (-1141.816) (-1139.795) (-1143.500) [-1143.286] * (-1148.811) (-1148.387) (-1146.691) [-1145.024] -- 0:01:38 508000 -- (-1146.943) (-1143.171) [-1141.761] (-1141.895) * (-1146.640) (-1149.900) (-1150.868) [-1141.418] -- 0:01:37 509000 -- (-1147.210) (-1142.377) [-1142.604] (-1141.981) * (-1143.256) (-1146.456) (-1146.673) [-1140.870] -- 0:01:37 510000 -- (-1137.377) (-1157.415) [-1147.093] (-1144.429) * (-1148.631) (-1147.362) [-1143.025] (-1143.656) -- 0:01:38 Average standard deviation of split frequencies: 0.018257 511000 -- (-1148.266) (-1150.172) [-1147.213] (-1151.312) * (-1159.084) [-1148.689] (-1145.870) (-1140.209) -- 0:01:37 512000 -- (-1144.459) (-1154.335) [-1139.776] (-1141.984) * (-1144.170) [-1146.595] (-1144.591) (-1142.835) -- 0:01:37 513000 -- (-1146.762) (-1144.776) [-1140.104] (-1151.440) * (-1145.872) [-1145.671] (-1144.050) (-1139.793) -- 0:01:36 514000 -- (-1149.194) [-1149.976] (-1143.815) (-1146.036) * (-1140.630) (-1144.479) [-1142.688] (-1142.867) -- 0:01:36 515000 -- (-1140.618) (-1145.067) [-1140.948] (-1143.959) * [-1140.495] (-1139.992) (-1146.820) (-1144.457) -- 0:01:37 Average standard deviation of split frequencies: 0.018982 516000 -- (-1142.302) [-1142.438] (-1140.870) (-1140.406) * (-1139.169) (-1146.890) (-1153.256) [-1140.772] -- 0:01:36 517000 -- [-1145.242] (-1152.800) (-1153.936) (-1138.698) * [-1141.238] (-1145.269) (-1148.368) (-1149.929) -- 0:01:36 518000 -- [-1139.977] (-1150.249) (-1143.471) (-1141.082) * (-1143.128) [-1142.437] (-1139.946) (-1147.718) -- 0:01:35 519000 -- [-1147.646] (-1145.777) (-1139.914) (-1148.691) * (-1142.237) [-1141.688] (-1147.540) (-1145.405) -- 0:01:36 520000 -- (-1145.699) (-1144.616) [-1139.953] (-1140.907) * [-1140.022] (-1142.555) (-1158.954) (-1143.941) -- 0:01:36 Average standard deviation of split frequencies: 0.019214 521000 -- (-1143.852) [-1148.900] (-1145.467) (-1142.590) * [-1144.167] (-1151.813) (-1142.980) (-1140.515) -- 0:01:35 522000 -- (-1143.295) (-1146.158) [-1148.370] (-1144.673) * [-1145.440] (-1148.348) (-1141.670) (-1139.068) -- 0:01:35 523000 -- (-1141.078) (-1140.775) (-1151.682) [-1135.456] * (-1155.401) (-1149.281) [-1138.338] (-1146.778) -- 0:01:34 524000 -- (-1150.547) [-1154.870] (-1144.226) (-1144.661) * (-1150.890) [-1143.529] (-1148.384) (-1145.827) -- 0:01:35 525000 -- (-1146.563) (-1142.251) (-1147.908) [-1151.083] * (-1140.686) [-1143.352] (-1147.957) (-1141.375) -- 0:01:35 Average standard deviation of split frequencies: 0.019418 526000 -- (-1151.440) [-1138.762] (-1139.948) (-1137.522) * (-1149.705) (-1144.708) [-1143.956] (-1147.138) -- 0:01:34 527000 -- (-1148.217) [-1142.380] (-1141.592) (-1147.321) * [-1141.617] (-1144.775) (-1146.780) (-1142.239) -- 0:01:34 528000 -- (-1158.918) (-1143.752) (-1139.797) [-1142.264] * (-1141.616) (-1146.935) [-1141.989] (-1141.297) -- 0:01:33 529000 -- (-1152.831) (-1145.041) (-1140.390) [-1143.343] * (-1145.525) [-1141.605] (-1144.868) (-1146.166) -- 0:01:34 530000 -- (-1140.042) (-1149.883) (-1140.697) [-1142.656] * [-1142.362] (-1141.948) (-1142.863) (-1138.734) -- 0:01:34 Average standard deviation of split frequencies: 0.019050 531000 -- (-1148.997) [-1146.164] (-1142.300) (-1142.466) * [-1142.487] (-1145.995) (-1151.034) (-1147.344) -- 0:01:33 532000 -- [-1144.377] (-1139.953) (-1148.380) (-1143.056) * (-1142.036) [-1140.384] (-1146.539) (-1144.684) -- 0:01:33 533000 -- (-1143.081) (-1154.050) (-1149.220) [-1142.978] * (-1142.573) (-1149.243) [-1140.685] (-1146.807) -- 0:01:32 534000 -- [-1141.401] (-1147.881) (-1138.735) (-1141.954) * (-1149.172) (-1144.175) (-1146.536) [-1141.871] -- 0:01:33 535000 -- [-1139.372] (-1138.961) (-1147.498) (-1142.316) * (-1143.114) (-1145.285) (-1143.107) [-1142.739] -- 0:01:33 Average standard deviation of split frequencies: 0.020619 536000 -- (-1139.699) (-1139.026) [-1138.133] (-1140.627) * (-1139.796) [-1140.727] (-1148.907) (-1151.461) -- 0:01:32 537000 -- [-1142.517] (-1139.669) (-1141.996) (-1138.159) * (-1144.290) [-1147.320] (-1147.527) (-1143.247) -- 0:01:32 538000 -- (-1147.307) (-1144.916) [-1142.047] (-1144.755) * (-1145.017) (-1149.667) [-1153.629] (-1141.640) -- 0:01:31 539000 -- (-1144.834) [-1140.905] (-1138.627) (-1145.734) * (-1147.204) (-1144.480) (-1144.424) [-1137.420] -- 0:01:32 540000 -- [-1142.208] (-1141.877) (-1141.352) (-1145.458) * (-1141.773) (-1151.955) (-1139.834) [-1140.821] -- 0:01:32 Average standard deviation of split frequencies: 0.020150 541000 -- (-1144.619) (-1152.705) [-1149.131] (-1146.502) * (-1143.809) (-1155.955) (-1148.798) [-1139.492] -- 0:01:31 542000 -- (-1145.716) (-1153.364) [-1142.439] (-1146.183) * (-1150.592) (-1152.997) [-1139.913] (-1149.111) -- 0:01:31 543000 -- (-1146.639) (-1143.166) (-1146.510) [-1147.203] * (-1149.056) (-1148.497) [-1143.093] (-1143.105) -- 0:01:30 544000 -- (-1148.478) [-1147.259] (-1140.606) (-1146.064) * [-1142.375] (-1143.940) (-1144.771) (-1145.857) -- 0:01:31 545000 -- (-1144.972) [-1142.648] (-1140.592) (-1141.481) * (-1139.731) (-1146.151) (-1144.464) [-1145.843] -- 0:01:31 Average standard deviation of split frequencies: 0.020433 546000 -- (-1154.873) [-1146.036] (-1140.286) (-1147.713) * (-1146.945) (-1144.164) [-1144.537] (-1145.460) -- 0:01:30 547000 -- (-1148.056) [-1140.816] (-1147.359) (-1142.942) * (-1144.459) (-1138.051) (-1141.747) [-1146.288] -- 0:01:30 548000 -- (-1154.410) (-1138.742) [-1140.249] (-1150.661) * (-1149.913) [-1138.978] (-1144.625) (-1147.519) -- 0:01:29 549000 -- (-1139.837) (-1144.687) [-1139.160] (-1139.291) * (-1142.821) (-1150.068) [-1142.331] (-1148.325) -- 0:01:29 550000 -- (-1149.684) (-1138.802) (-1136.977) [-1145.158] * (-1146.520) (-1142.991) [-1140.480] (-1151.511) -- 0:01:30 Average standard deviation of split frequencies: 0.020736 551000 -- (-1143.923) (-1147.967) (-1146.321) [-1145.888] * (-1143.209) [-1143.394] (-1137.955) (-1154.295) -- 0:01:29 552000 -- [-1144.871] (-1145.005) (-1143.314) (-1148.649) * (-1145.005) (-1141.280) (-1147.743) [-1142.074] -- 0:01:29 553000 -- (-1148.418) (-1142.701) (-1141.489) [-1144.376] * (-1146.906) (-1140.250) [-1153.757] (-1142.841) -- 0:01:28 554000 -- (-1143.752) (-1138.504) (-1139.819) [-1141.224] * (-1142.299) [-1142.972] (-1147.049) (-1143.157) -- 0:01:28 555000 -- (-1140.914) (-1146.385) [-1144.024] (-1143.550) * (-1157.922) (-1150.630) [-1141.018] (-1145.122) -- 0:01:29 Average standard deviation of split frequencies: 0.020819 556000 -- [-1141.724] (-1147.885) (-1149.517) (-1149.920) * (-1144.249) (-1145.833) [-1149.267] (-1150.952) -- 0:01:28 557000 -- (-1146.588) (-1153.078) (-1146.349) [-1137.524] * (-1145.033) (-1141.612) [-1145.586] (-1145.388) -- 0:01:28 558000 -- (-1141.819) [-1142.516] (-1141.997) (-1142.351) * (-1145.339) (-1141.565) [-1145.023] (-1141.828) -- 0:01:27 559000 -- (-1138.274) (-1147.534) (-1143.873) [-1143.912] * (-1139.092) (-1143.569) (-1146.877) [-1139.931] -- 0:01:27 560000 -- (-1147.517) (-1150.013) (-1142.273) [-1143.530] * (-1139.322) (-1144.276) [-1150.482] (-1146.676) -- 0:01:28 Average standard deviation of split frequencies: 0.019712 561000 -- (-1140.300) (-1145.888) (-1139.755) [-1139.241] * (-1146.670) (-1142.385) [-1150.015] (-1140.772) -- 0:01:27 562000 -- (-1148.081) (-1151.298) (-1150.203) [-1136.948] * (-1147.731) (-1148.427) (-1149.455) [-1139.622] -- 0:01:27 563000 -- (-1141.889) (-1147.177) (-1148.781) [-1143.865] * (-1139.553) (-1146.010) [-1149.910] (-1141.062) -- 0:01:26 564000 -- (-1140.640) [-1144.076] (-1147.693) (-1138.830) * [-1146.892] (-1151.146) (-1141.724) (-1147.565) -- 0:01:27 565000 -- (-1142.685) [-1143.676] (-1144.499) (-1139.736) * (-1149.579) (-1140.759) [-1139.210] (-1146.960) -- 0:01:27 Average standard deviation of split frequencies: 0.020452 566000 -- (-1146.014) (-1140.218) (-1144.177) [-1146.806] * (-1146.925) [-1142.830] (-1151.601) (-1146.163) -- 0:01:26 567000 -- (-1147.332) (-1149.499) [-1143.485] (-1142.516) * (-1148.062) [-1138.704] (-1144.679) (-1147.770) -- 0:01:26 568000 -- (-1148.682) [-1149.136] (-1148.176) (-1143.827) * [-1145.810] (-1152.452) (-1145.561) (-1141.231) -- 0:01:25 569000 -- (-1145.485) [-1143.752] (-1149.720) (-1153.992) * [-1144.083] (-1154.048) (-1143.574) (-1140.157) -- 0:01:25 570000 -- (-1143.249) (-1140.595) (-1141.153) [-1147.792] * (-1148.290) (-1146.212) (-1144.607) [-1142.672] -- 0:01:26 Average standard deviation of split frequencies: 0.021019 571000 -- [-1139.650] (-1138.636) (-1140.333) (-1138.136) * (-1150.402) [-1146.619] (-1141.962) (-1148.223) -- 0:01:25 572000 -- (-1143.376) [-1141.848] (-1137.434) (-1138.915) * (-1149.356) (-1143.885) (-1145.957) [-1141.049] -- 0:01:25 573000 -- [-1140.827] (-1145.460) (-1151.147) (-1142.073) * (-1160.168) (-1144.199) [-1142.387] (-1144.052) -- 0:01:24 574000 -- (-1143.362) (-1141.387) [-1144.053] (-1149.274) * (-1149.557) [-1140.767] (-1147.971) (-1146.910) -- 0:01:24 575000 -- (-1149.867) (-1137.032) (-1144.646) [-1144.621] * (-1146.932) (-1142.151) (-1153.373) [-1145.803] -- 0:01:25 Average standard deviation of split frequencies: 0.021551 576000 -- (-1144.923) [-1136.644] (-1141.681) (-1141.138) * (-1148.346) [-1140.860] (-1145.729) (-1143.621) -- 0:01:24 577000 -- [-1150.815] (-1148.037) (-1144.813) (-1142.802) * (-1144.936) [-1142.844] (-1144.257) (-1145.436) -- 0:01:24 578000 -- (-1145.100) [-1138.373] (-1144.924) (-1143.410) * (-1147.188) [-1144.710] (-1147.877) (-1145.547) -- 0:01:23 579000 -- (-1146.283) [-1142.834] (-1149.543) (-1145.444) * (-1141.636) (-1141.565) (-1143.067) [-1139.367] -- 0:01:23 580000 -- (-1152.537) (-1142.264) (-1136.277) [-1149.838] * (-1145.858) (-1142.291) [-1138.530] (-1141.897) -- 0:01:24 Average standard deviation of split frequencies: 0.020657 581000 -- (-1148.350) [-1140.171] (-1147.139) (-1149.180) * [-1139.571] (-1144.350) (-1139.334) (-1142.983) -- 0:01:23 582000 -- (-1140.141) [-1137.112] (-1138.357) (-1145.310) * (-1140.299) (-1145.643) (-1146.424) [-1141.558] -- 0:01:23 583000 -- (-1139.831) (-1150.896) [-1146.225] (-1147.214) * [-1143.148] (-1141.024) (-1142.035) (-1147.707) -- 0:01:22 584000 -- [-1143.626] (-1155.701) (-1144.398) (-1144.049) * [-1142.375] (-1144.983) (-1150.515) (-1139.862) -- 0:01:22 585000 -- (-1141.314) (-1146.365) (-1151.643) [-1140.996] * (-1142.814) (-1145.483) (-1144.171) [-1148.107] -- 0:01:23 Average standard deviation of split frequencies: 0.020558 586000 -- [-1146.502] (-1144.323) (-1139.815) (-1144.179) * (-1141.799) [-1144.884] (-1145.674) (-1149.756) -- 0:01:22 587000 -- (-1140.172) [-1137.234] (-1140.816) (-1143.755) * (-1137.248) (-1146.903) (-1143.683) [-1146.887] -- 0:01:22 588000 -- (-1139.034) (-1147.434) (-1143.198) [-1141.459] * (-1144.750) (-1149.332) (-1141.404) [-1149.715] -- 0:01:21 589000 -- (-1147.930) (-1144.059) (-1137.867) [-1141.241] * (-1140.087) (-1151.617) (-1142.075) [-1143.922] -- 0:01:21 590000 -- [-1144.867] (-1142.770) (-1144.772) (-1143.952) * (-1143.118) (-1145.407) (-1146.711) [-1139.528] -- 0:01:22 Average standard deviation of split frequencies: 0.021016 591000 -- (-1139.449) (-1145.036) (-1140.841) [-1147.196] * (-1142.301) [-1146.857] (-1145.121) (-1150.852) -- 0:01:21 592000 -- [-1143.205] (-1149.494) (-1141.299) (-1140.408) * (-1148.317) (-1142.995) (-1137.550) [-1140.359] -- 0:01:21 593000 -- (-1138.352) (-1140.869) (-1148.647) [-1145.961] * (-1140.281) [-1142.898] (-1152.117) (-1142.767) -- 0:01:20 594000 -- (-1141.423) (-1148.672) [-1137.878] (-1148.465) * (-1140.274) (-1137.080) (-1149.049) [-1141.088] -- 0:01:20 595000 -- (-1145.578) (-1145.467) (-1145.838) [-1147.815] * (-1139.251) (-1140.315) [-1141.982] (-1143.737) -- 0:01:21 Average standard deviation of split frequencies: 0.021004 596000 -- (-1141.344) (-1147.253) (-1155.169) [-1147.747] * [-1144.385] (-1142.186) (-1138.716) (-1141.070) -- 0:01:20 597000 -- [-1137.205] (-1144.676) (-1146.227) (-1145.375) * (-1152.886) [-1147.230] (-1144.435) (-1144.664) -- 0:01:20 598000 -- [-1140.872] (-1143.443) (-1144.467) (-1145.057) * (-1143.383) [-1147.089] (-1140.459) (-1140.025) -- 0:01:19 599000 -- [-1138.309] (-1142.348) (-1146.464) (-1155.133) * (-1144.123) (-1141.792) (-1138.298) [-1139.921] -- 0:01:19 600000 -- [-1140.418] (-1146.733) (-1140.712) (-1150.398) * (-1147.474) [-1143.202] (-1147.028) (-1142.745) -- 0:01:20 Average standard deviation of split frequencies: 0.020492 601000 -- (-1149.114) [-1139.595] (-1146.623) (-1149.777) * (-1144.706) (-1148.903) [-1144.174] (-1150.858) -- 0:01:19 602000 -- (-1140.302) (-1138.153) [-1136.741] (-1152.054) * (-1151.833) (-1142.422) (-1141.441) [-1135.046] -- 0:01:19 603000 -- (-1144.086) (-1141.080) [-1139.621] (-1149.528) * (-1146.436) (-1138.419) [-1141.181] (-1150.100) -- 0:01:19 604000 -- (-1143.882) (-1137.847) (-1145.251) [-1138.330] * (-1146.653) (-1145.627) (-1150.072) [-1143.102] -- 0:01:18 605000 -- [-1152.284] (-1144.550) (-1143.858) (-1151.139) * [-1146.370] (-1138.641) (-1141.577) (-1150.378) -- 0:01:19 Average standard deviation of split frequencies: 0.019966 606000 -- (-1142.579) (-1138.353) [-1139.229] (-1150.964) * (-1139.109) [-1138.239] (-1146.235) (-1151.274) -- 0:01:18 607000 -- (-1147.838) [-1139.457] (-1142.946) (-1147.349) * [-1140.294] (-1151.974) (-1147.387) (-1152.813) -- 0:01:18 608000 -- (-1138.145) (-1144.640) (-1155.885) [-1146.151] * [-1140.083] (-1140.356) (-1156.095) (-1142.912) -- 0:01:18 609000 -- (-1144.775) (-1143.824) [-1139.152] (-1147.029) * [-1139.795] (-1141.340) (-1141.149) (-1138.170) -- 0:01:17 610000 -- (-1146.810) (-1138.836) (-1144.752) [-1141.025] * (-1142.691) (-1141.877) (-1145.748) [-1142.545] -- 0:01:18 Average standard deviation of split frequencies: 0.019556 611000 -- (-1146.102) [-1141.377] (-1139.515) (-1147.929) * (-1145.813) [-1137.719] (-1143.158) (-1150.930) -- 0:01:17 612000 -- (-1155.704) (-1144.835) [-1141.339] (-1140.393) * [-1144.526] (-1151.482) (-1140.552) (-1140.659) -- 0:01:17 613000 -- [-1142.193] (-1139.736) (-1140.678) (-1145.483) * (-1147.162) (-1140.047) (-1147.386) [-1143.704] -- 0:01:17 614000 -- (-1142.693) (-1145.037) (-1141.685) [-1139.400] * (-1144.889) (-1144.055) [-1141.758] (-1142.606) -- 0:01:16 615000 -- [-1144.480] (-1145.016) (-1149.252) (-1144.686) * (-1143.187) (-1145.440) (-1145.931) [-1136.624] -- 0:01:17 Average standard deviation of split frequencies: 0.019472 616000 -- (-1150.064) (-1150.938) (-1145.520) [-1144.316] * (-1138.591) (-1147.562) (-1148.367) [-1139.868] -- 0:01:16 617000 -- [-1141.189] (-1155.593) (-1145.512) (-1152.308) * (-1139.746) (-1148.747) (-1142.368) [-1139.000] -- 0:01:16 618000 -- [-1144.802] (-1144.658) (-1147.066) (-1140.292) * (-1150.422) (-1141.306) (-1146.500) [-1142.705] -- 0:01:16 619000 -- (-1150.814) (-1141.676) (-1147.724) [-1141.302] * (-1146.571) (-1138.860) (-1146.706) [-1143.634] -- 0:01:15 620000 -- [-1145.105] (-1141.228) (-1143.225) (-1146.428) * (-1147.494) [-1144.011] (-1142.046) (-1144.329) -- 0:01:16 Average standard deviation of split frequencies: 0.018988 621000 -- (-1155.207) (-1149.682) [-1139.344] (-1151.026) * [-1144.572] (-1144.083) (-1144.294) (-1148.030) -- 0:01:15 622000 -- (-1142.320) (-1149.258) (-1144.986) [-1140.966] * [-1140.564] (-1145.777) (-1144.188) (-1152.791) -- 0:01:15 623000 -- [-1145.717] (-1149.129) (-1138.121) (-1151.585) * (-1139.115) (-1147.815) (-1145.052) [-1142.236] -- 0:01:15 624000 -- [-1142.821] (-1141.581) (-1145.826) (-1143.088) * (-1146.861) (-1137.734) (-1146.322) [-1138.467] -- 0:01:14 625000 -- [-1142.834] (-1144.164) (-1152.810) (-1139.859) * (-1141.589) (-1149.271) [-1145.073] (-1145.314) -- 0:01:15 Average standard deviation of split frequencies: 0.018491 626000 -- (-1142.427) (-1143.225) [-1145.989] (-1146.379) * [-1139.373] (-1142.748) (-1143.400) (-1136.977) -- 0:01:14 627000 -- (-1143.496) (-1137.998) (-1151.226) [-1144.490] * (-1152.558) (-1141.351) (-1145.941) [-1140.091] -- 0:01:14 628000 -- (-1140.113) (-1146.103) [-1141.640] (-1152.406) * [-1139.769] (-1143.196) (-1140.203) (-1145.263) -- 0:01:14 629000 -- (-1141.841) (-1146.935) (-1149.049) [-1142.885] * (-1141.413) [-1137.394] (-1139.542) (-1146.231) -- 0:01:13 630000 -- (-1142.410) (-1147.682) [-1140.592] (-1141.683) * (-1142.905) [-1140.166] (-1144.165) (-1145.317) -- 0:01:14 Average standard deviation of split frequencies: 0.018521 631000 -- (-1143.526) (-1142.327) [-1140.431] (-1143.205) * (-1142.434) (-1144.816) [-1142.736] (-1142.465) -- 0:01:13 632000 -- (-1138.985) (-1150.742) [-1137.614] (-1151.272) * (-1142.697) (-1145.588) (-1146.290) [-1146.689] -- 0:01:13 633000 -- (-1145.318) (-1142.767) [-1142.728] (-1143.293) * (-1139.225) [-1145.839] (-1141.999) (-1145.866) -- 0:01:13 634000 -- [-1148.910] (-1144.184) (-1150.253) (-1139.969) * [-1139.047] (-1141.164) (-1149.867) (-1144.199) -- 0:01:12 635000 -- (-1141.725) [-1148.903] (-1143.785) (-1145.395) * (-1146.622) [-1144.531] (-1140.648) (-1141.817) -- 0:01:13 Average standard deviation of split frequencies: 0.018201 636000 -- (-1144.579) [-1144.418] (-1146.078) (-1141.120) * [-1139.281] (-1143.905) (-1152.910) (-1148.177) -- 0:01:12 637000 -- (-1140.374) (-1142.753) (-1149.417) [-1151.186] * (-1145.370) (-1148.626) [-1147.252] (-1146.292) -- 0:01:12 638000 -- (-1138.221) [-1138.405] (-1139.358) (-1147.727) * (-1145.223) [-1141.176] (-1145.992) (-1141.953) -- 0:01:12 639000 -- (-1139.798) [-1145.620] (-1140.969) (-1140.688) * [-1144.272] (-1147.732) (-1153.922) (-1142.775) -- 0:01:11 640000 -- [-1139.327] (-1143.700) (-1139.440) (-1144.085) * (-1149.637) (-1151.973) (-1139.244) [-1140.242] -- 0:01:12 Average standard deviation of split frequencies: 0.018640 641000 -- (-1142.861) [-1138.114] (-1146.993) (-1153.111) * (-1145.326) (-1147.400) [-1144.491] (-1139.095) -- 0:01:11 642000 -- (-1148.178) (-1145.115) [-1146.008] (-1140.284) * (-1142.502) (-1140.897) [-1150.570] (-1141.057) -- 0:01:11 643000 -- (-1150.473) [-1141.410] (-1143.177) (-1145.800) * (-1150.088) (-1139.420) [-1147.989] (-1141.611) -- 0:01:11 644000 -- (-1142.100) (-1142.349) [-1135.719] (-1145.501) * (-1152.822) [-1138.418] (-1143.648) (-1147.740) -- 0:01:10 645000 -- (-1148.822) (-1143.798) [-1140.805] (-1141.441) * [-1144.555] (-1144.352) (-1140.281) (-1145.928) -- 0:01:11 Average standard deviation of split frequencies: 0.018324 646000 -- (-1152.403) [-1143.552] (-1143.851) (-1143.811) * (-1150.781) (-1140.057) (-1144.016) [-1142.032] -- 0:01:10 647000 -- (-1156.478) [-1147.904] (-1142.611) (-1141.534) * [-1149.275] (-1144.587) (-1149.702) (-1141.437) -- 0:01:10 648000 -- (-1150.654) (-1145.009) [-1143.131] (-1144.331) * [-1145.373] (-1148.208) (-1137.381) (-1148.536) -- 0:01:10 649000 -- [-1147.182] (-1150.622) (-1136.551) (-1146.990) * (-1140.742) (-1145.657) (-1145.436) [-1142.462] -- 0:01:09 650000 -- (-1150.966) [-1148.440] (-1138.311) (-1140.235) * (-1146.986) (-1146.232) [-1142.037] (-1141.194) -- 0:01:10 Average standard deviation of split frequencies: 0.018112 651000 -- (-1146.751) (-1143.531) (-1141.347) [-1142.189] * (-1143.282) (-1151.108) (-1143.671) [-1143.124] -- 0:01:09 652000 -- (-1142.830) [-1148.388] (-1144.946) (-1143.987) * (-1141.843) (-1156.226) [-1139.982] (-1142.750) -- 0:01:09 653000 -- (-1151.406) (-1146.580) [-1145.255] (-1146.140) * (-1143.918) (-1149.750) [-1140.153] (-1144.396) -- 0:01:09 654000 -- (-1147.794) (-1143.474) [-1145.623] (-1144.939) * (-1143.832) [-1141.045] (-1147.207) (-1157.315) -- 0:01:08 655000 -- [-1143.654] (-1148.127) (-1141.070) (-1147.285) * [-1142.274] (-1147.190) (-1140.029) (-1143.654) -- 0:01:09 Average standard deviation of split frequencies: 0.016608 656000 -- (-1143.776) (-1148.754) (-1145.644) [-1141.889] * [-1139.467] (-1144.501) (-1150.728) (-1142.698) -- 0:01:08 657000 -- (-1152.001) (-1143.337) (-1152.511) [-1146.445] * (-1142.201) (-1147.708) (-1142.922) [-1142.844] -- 0:01:08 658000 -- (-1149.012) (-1142.577) (-1140.508) [-1146.706] * (-1141.973) [-1146.907] (-1138.997) (-1145.104) -- 0:01:08 659000 -- (-1146.947) (-1145.872) [-1141.698] (-1144.237) * (-1142.943) (-1148.408) [-1141.179] (-1146.627) -- 0:01:07 660000 -- (-1141.459) [-1140.338] (-1142.103) (-1151.836) * (-1150.439) (-1156.571) (-1143.194) [-1139.243] -- 0:01:08 Average standard deviation of split frequencies: 0.016015 661000 -- (-1138.810) (-1142.064) [-1141.542] (-1145.731) * [-1145.343] (-1150.096) (-1147.377) (-1147.879) -- 0:01:07 662000 -- [-1142.096] (-1149.054) (-1150.798) (-1145.499) * (-1143.535) [-1143.702] (-1142.677) (-1149.610) -- 0:01:07 663000 -- (-1144.825) (-1139.841) (-1139.705) [-1145.789] * (-1145.463) (-1148.125) [-1136.347] (-1141.575) -- 0:01:07 664000 -- (-1137.978) (-1149.037) [-1146.285] (-1153.727) * (-1146.613) (-1144.590) [-1144.548] (-1143.937) -- 0:01:06 665000 -- (-1143.883) (-1149.482) [-1141.556] (-1139.717) * (-1151.429) (-1146.838) (-1139.363) [-1140.788] -- 0:01:07 Average standard deviation of split frequencies: 0.015965 666000 -- (-1151.248) [-1142.489] (-1139.210) (-1152.560) * (-1143.841) (-1144.246) [-1139.903] (-1145.244) -- 0:01:06 667000 -- [-1141.892] (-1146.873) (-1154.601) (-1140.483) * (-1147.160) (-1145.422) [-1140.363] (-1141.864) -- 0:01:06 668000 -- (-1146.976) [-1144.590] (-1150.615) (-1138.597) * (-1142.655) (-1146.686) [-1141.533] (-1151.696) -- 0:01:06 669000 -- (-1144.306) [-1148.563] (-1147.101) (-1147.345) * (-1142.917) (-1140.850) (-1149.706) [-1141.902] -- 0:01:05 670000 -- [-1143.916] (-1143.166) (-1148.957) (-1151.333) * (-1145.371) [-1139.911] (-1139.797) (-1141.238) -- 0:01:06 Average standard deviation of split frequencies: 0.016869 671000 -- (-1144.498) (-1145.725) (-1145.222) [-1151.992] * (-1138.744) (-1140.731) [-1136.989] (-1153.264) -- 0:01:05 672000 -- [-1140.441] (-1152.773) (-1142.562) (-1145.800) * (-1146.884) (-1147.322) (-1148.942) [-1145.162] -- 0:01:05 673000 -- [-1139.104] (-1144.447) (-1152.296) (-1142.143) * (-1151.257) (-1146.708) [-1141.830] (-1149.833) -- 0:01:05 674000 -- [-1142.334] (-1141.421) (-1138.788) (-1148.088) * [-1149.759] (-1144.170) (-1139.025) (-1140.539) -- 0:01:04 675000 -- (-1145.824) (-1137.833) [-1140.375] (-1151.026) * (-1147.898) [-1141.218] (-1139.861) (-1150.120) -- 0:01:05 Average standard deviation of split frequencies: 0.015961 676000 -- (-1139.926) [-1144.053] (-1140.363) (-1146.608) * (-1144.689) (-1143.071) [-1145.680] (-1143.589) -- 0:01:04 677000 -- (-1148.454) [-1140.439] (-1139.485) (-1149.596) * (-1143.787) (-1141.358) [-1144.413] (-1141.616) -- 0:01:04 678000 -- (-1143.002) (-1143.113) [-1137.842] (-1148.119) * (-1140.714) (-1144.849) (-1150.990) [-1145.491] -- 0:01:04 679000 -- (-1143.367) (-1142.776) (-1148.494) [-1146.259] * (-1143.166) (-1140.859) [-1141.653] (-1145.839) -- 0:01:03 680000 -- [-1139.416] (-1146.267) (-1149.559) (-1147.636) * (-1149.923) [-1145.828] (-1149.140) (-1141.202) -- 0:01:04 Average standard deviation of split frequencies: 0.016160 681000 -- [-1139.894] (-1147.851) (-1137.903) (-1146.401) * (-1144.948) (-1147.727) (-1145.676) [-1146.669] -- 0:01:03 682000 -- (-1138.540) (-1139.283) [-1146.491] (-1160.530) * (-1139.125) (-1140.466) [-1139.633] (-1146.028) -- 0:01:03 683000 -- (-1142.686) (-1144.422) (-1146.369) [-1145.010] * (-1150.959) [-1140.188] (-1143.992) (-1145.759) -- 0:01:03 684000 -- (-1145.049) (-1143.339) (-1147.421) [-1147.538] * (-1144.123) (-1147.844) (-1146.049) [-1138.536] -- 0:01:02 685000 -- [-1141.953] (-1140.447) (-1142.960) (-1144.956) * (-1143.009) (-1142.269) [-1146.737] (-1145.167) -- 0:01:02 Average standard deviation of split frequencies: 0.015805 686000 -- (-1141.394) (-1147.724) [-1143.726] (-1148.825) * (-1139.122) (-1144.524) (-1142.536) [-1144.789] -- 0:01:02 687000 -- (-1145.307) [-1142.482] (-1145.613) (-1141.235) * [-1139.629] (-1140.085) (-1146.272) (-1144.389) -- 0:01:02 688000 -- [-1145.580] (-1143.548) (-1144.193) (-1149.277) * [-1143.235] (-1142.618) (-1142.969) (-1138.575) -- 0:01:02 689000 -- (-1140.744) (-1151.871) (-1143.017) [-1140.186] * [-1138.641] (-1144.758) (-1141.809) (-1143.003) -- 0:01:01 690000 -- (-1144.155) (-1144.355) [-1141.103] (-1149.154) * (-1145.759) (-1141.424) (-1142.344) [-1144.232] -- 0:01:01 Average standard deviation of split frequencies: 0.015698 691000 -- (-1141.589) (-1143.251) (-1146.030) [-1144.765] * [-1145.279] (-1144.978) (-1143.808) (-1143.145) -- 0:01:01 692000 -- (-1148.851) (-1141.401) [-1140.342] (-1145.046) * (-1143.258) (-1144.021) (-1142.625) [-1148.012] -- 0:01:01 693000 -- (-1142.458) [-1141.366] (-1150.545) (-1141.194) * [-1146.672] (-1142.262) (-1140.133) (-1142.440) -- 0:01:01 694000 -- (-1148.211) (-1150.356) [-1139.594] (-1144.729) * (-1142.317) (-1147.205) (-1143.117) [-1142.381] -- 0:01:00 695000 -- (-1142.197) [-1142.484] (-1136.246) (-1144.066) * [-1145.638] (-1156.376) (-1146.189) (-1142.097) -- 0:01:00 Average standard deviation of split frequencies: 0.015879 696000 -- (-1142.273) (-1146.261) (-1144.549) [-1140.097] * [-1140.712] (-1141.020) (-1145.622) (-1143.526) -- 0:01:00 697000 -- (-1150.360) (-1148.232) (-1145.951) [-1145.077] * [-1143.530] (-1146.472) (-1145.259) (-1143.401) -- 0:01:00 698000 -- (-1142.074) (-1139.728) (-1138.843) [-1142.961] * [-1141.674] (-1142.755) (-1140.194) (-1149.161) -- 0:01:00 699000 -- (-1139.829) [-1138.503] (-1140.565) (-1144.829) * (-1153.637) [-1143.620] (-1140.927) (-1151.196) -- 0:00:59 700000 -- (-1151.044) (-1139.799) (-1137.045) [-1141.360] * (-1146.552) [-1142.875] (-1144.573) (-1148.262) -- 0:01:00 Average standard deviation of split frequencies: 0.015175 701000 -- [-1142.408] (-1140.880) (-1142.239) (-1140.412) * [-1144.688] (-1147.888) (-1144.653) (-1144.448) -- 0:00:59 702000 -- (-1147.456) (-1144.854) (-1144.112) [-1145.863] * (-1144.925) [-1146.503] (-1139.964) (-1147.873) -- 0:00:59 703000 -- (-1150.929) (-1144.383) (-1147.303) [-1140.131] * [-1143.984] (-1142.696) (-1148.799) (-1142.280) -- 0:00:59 704000 -- [-1140.428] (-1153.483) (-1142.504) (-1144.143) * (-1138.488) (-1145.328) (-1140.795) [-1145.256] -- 0:00:58 705000 -- (-1143.123) (-1151.680) (-1142.787) [-1136.369] * (-1143.110) [-1139.257] (-1145.653) (-1143.144) -- 0:00:59 Average standard deviation of split frequencies: 0.014912 706000 -- (-1140.692) (-1140.890) [-1139.503] (-1146.638) * (-1138.625) (-1153.766) (-1147.831) [-1141.610] -- 0:00:58 707000 -- (-1140.753) (-1139.672) (-1145.296) [-1136.799] * (-1138.736) (-1139.913) [-1140.469] (-1146.006) -- 0:00:58 708000 -- (-1144.477) [-1145.417] (-1147.276) (-1149.995) * (-1145.017) (-1141.244) [-1139.461] (-1150.973) -- 0:00:58 709000 -- (-1144.433) [-1137.570] (-1143.525) (-1143.641) * (-1140.588) [-1146.587] (-1141.434) (-1149.616) -- 0:00:57 710000 -- [-1143.812] (-1145.831) (-1152.609) (-1145.616) * (-1147.439) (-1150.004) (-1140.841) [-1146.678] -- 0:00:57 Average standard deviation of split frequencies: 0.014667 711000 -- (-1147.718) [-1145.476] (-1139.014) (-1144.120) * (-1143.409) (-1143.002) [-1146.084] (-1151.217) -- 0:00:57 712000 -- (-1148.861) (-1144.523) [-1138.968] (-1150.737) * [-1141.315] (-1139.285) (-1148.030) (-1148.452) -- 0:00:57 713000 -- (-1143.960) (-1151.767) [-1141.014] (-1140.979) * (-1144.216) (-1144.352) (-1139.181) [-1142.921] -- 0:00:57 714000 -- (-1141.846) (-1143.079) [-1139.683] (-1149.186) * (-1145.071) (-1139.493) (-1138.722) [-1138.619] -- 0:00:56 715000 -- (-1147.855) (-1143.716) [-1148.921] (-1148.811) * (-1140.163) (-1141.945) [-1144.570] (-1147.556) -- 0:00:57 Average standard deviation of split frequencies: 0.014923 716000 -- (-1144.510) [-1136.926] (-1140.625) (-1139.344) * [-1137.373] (-1141.877) (-1154.400) (-1139.852) -- 0:00:56 717000 -- (-1143.507) (-1141.533) (-1143.585) [-1142.068] * [-1142.953] (-1138.726) (-1139.085) (-1153.067) -- 0:00:56 718000 -- (-1140.715) [-1145.786] (-1148.192) (-1143.041) * (-1145.402) (-1148.100) [-1144.093] (-1144.775) -- 0:00:56 719000 -- (-1139.374) [-1140.441] (-1148.605) (-1140.571) * [-1145.363] (-1143.113) (-1144.292) (-1144.908) -- 0:00:55 720000 -- (-1145.303) (-1143.498) (-1144.477) [-1143.531] * (-1148.161) [-1141.066] (-1140.457) (-1147.972) -- 0:00:56 Average standard deviation of split frequencies: 0.014609 721000 -- (-1142.846) (-1142.086) (-1144.122) [-1142.941] * (-1152.585) (-1148.269) (-1146.085) [-1142.142] -- 0:00:55 722000 -- (-1148.827) [-1140.286] (-1148.830) (-1148.103) * [-1140.182] (-1139.505) (-1150.364) (-1145.522) -- 0:00:55 723000 -- (-1147.762) (-1149.070) (-1152.486) [-1141.559] * (-1140.908) (-1143.058) [-1138.055] (-1143.018) -- 0:00:55 724000 -- (-1148.683) (-1145.195) (-1147.548) [-1148.504] * (-1140.076) [-1142.430] (-1139.729) (-1150.880) -- 0:00:54 725000 -- (-1151.190) [-1146.740] (-1142.378) (-1143.091) * [-1142.195] (-1142.998) (-1141.765) (-1144.656) -- 0:00:55 Average standard deviation of split frequencies: 0.014429 726000 -- [-1141.744] (-1145.210) (-1144.524) (-1145.654) * [-1141.580] (-1147.364) (-1148.235) (-1150.597) -- 0:00:54 727000 -- (-1140.111) (-1142.327) (-1146.813) [-1143.597] * [-1140.246] (-1149.489) (-1143.930) (-1149.965) -- 0:00:54 728000 -- [-1144.584] (-1141.168) (-1139.162) (-1142.404) * (-1146.092) (-1146.273) [-1142.412] (-1138.063) -- 0:00:54 729000 -- [-1141.994] (-1143.950) (-1140.036) (-1141.639) * (-1146.872) (-1141.603) [-1141.814] (-1150.437) -- 0:00:53 730000 -- (-1134.164) [-1144.542] (-1143.870) (-1142.623) * (-1141.600) [-1142.574] (-1138.216) (-1146.013) -- 0:00:54 Average standard deviation of split frequencies: 0.015126 731000 -- (-1140.399) (-1142.435) [-1144.698] (-1146.065) * (-1141.861) [-1143.345] (-1142.480) (-1144.393) -- 0:00:53 732000 -- [-1146.582] (-1146.762) (-1146.190) (-1148.182) * (-1147.170) (-1148.834) [-1142.022] (-1142.911) -- 0:00:53 733000 -- (-1148.693) (-1141.696) (-1149.532) [-1142.632] * (-1145.547) (-1147.817) [-1141.020] (-1141.728) -- 0:00:53 734000 -- (-1146.974) (-1145.132) [-1141.840] (-1154.309) * (-1153.901) (-1147.693) [-1137.121] (-1145.259) -- 0:00:52 735000 -- (-1139.257) [-1143.812] (-1149.882) (-1144.092) * (-1154.891) [-1143.772] (-1145.797) (-1144.627) -- 0:00:53 Average standard deviation of split frequencies: 0.014731 736000 -- (-1146.963) [-1143.240] (-1144.730) (-1141.374) * (-1137.964) (-1142.795) [-1140.836] (-1140.055) -- 0:00:52 737000 -- (-1143.690) (-1153.193) [-1141.911] (-1147.437) * [-1142.857] (-1149.366) (-1150.127) (-1144.985) -- 0:00:52 738000 -- [-1144.930] (-1143.105) (-1142.391) (-1147.476) * (-1142.634) (-1147.780) (-1148.028) [-1149.113] -- 0:00:52 739000 -- (-1155.623) (-1143.155) (-1137.677) [-1141.768] * (-1138.922) [-1142.488] (-1143.916) (-1143.272) -- 0:00:51 740000 -- [-1144.564] (-1138.959) (-1152.123) (-1145.844) * (-1144.647) (-1150.646) [-1139.015] (-1147.421) -- 0:00:52 Average standard deviation of split frequencies: 0.014144 741000 -- (-1145.394) [-1141.200] (-1139.299) (-1142.230) * (-1143.060) (-1143.352) [-1146.385] (-1148.692) -- 0:00:51 742000 -- (-1148.403) (-1139.390) [-1141.850] (-1144.632) * [-1141.289] (-1145.980) (-1146.657) (-1145.817) -- 0:00:51 743000 -- (-1145.247) (-1146.932) (-1142.235) [-1144.333] * [-1142.732] (-1136.512) (-1147.517) (-1146.470) -- 0:00:51 744000 -- (-1144.209) [-1138.363] (-1141.977) (-1147.843) * (-1148.674) (-1142.706) (-1138.363) [-1145.518] -- 0:00:50 745000 -- (-1143.935) (-1142.508) (-1142.246) [-1144.791] * (-1140.026) (-1145.260) (-1139.922) [-1145.238] -- 0:00:51 Average standard deviation of split frequencies: 0.014604 746000 -- (-1147.734) (-1147.685) (-1143.628) [-1144.201] * (-1146.173) (-1145.375) (-1144.575) [-1139.508] -- 0:00:50 747000 -- (-1141.300) (-1143.517) [-1143.675] (-1145.758) * [-1146.844] (-1140.714) (-1148.962) (-1139.686) -- 0:00:50 748000 -- (-1143.769) [-1141.721] (-1144.671) (-1142.610) * (-1145.724) (-1140.627) [-1140.507] (-1147.689) -- 0:00:50 749000 -- (-1140.755) [-1142.560] (-1148.839) (-1140.009) * (-1147.094) (-1145.412) [-1143.048] (-1144.400) -- 0:00:49 750000 -- (-1150.752) (-1142.204) [-1143.058] (-1144.717) * (-1140.096) [-1146.190] (-1145.994) (-1141.365) -- 0:00:50 Average standard deviation of split frequencies: 0.014583 751000 -- (-1136.925) (-1141.583) [-1140.467] (-1142.933) * (-1140.448) (-1144.993) [-1144.804] (-1147.683) -- 0:00:49 752000 -- (-1150.970) (-1144.011) [-1143.116] (-1150.317) * (-1144.117) [-1138.674] (-1150.438) (-1143.190) -- 0:00:49 753000 -- (-1146.047) (-1138.438) (-1152.224) [-1149.817] * (-1145.345) [-1146.457] (-1141.304) (-1144.486) -- 0:00:49 754000 -- (-1150.003) (-1142.367) (-1142.099) [-1142.789] * (-1152.821) [-1144.900] (-1137.198) (-1143.942) -- 0:00:48 755000 -- [-1141.425] (-1140.649) (-1145.943) (-1144.562) * (-1145.290) [-1140.506] (-1148.317) (-1144.067) -- 0:00:49 Average standard deviation of split frequencies: 0.014134 756000 -- (-1147.195) (-1146.965) [-1143.678] (-1144.318) * (-1150.217) (-1143.119) (-1146.530) [-1145.056] -- 0:00:48 757000 -- (-1142.236) (-1142.069) [-1139.448] (-1142.270) * (-1145.067) (-1139.096) (-1152.720) [-1144.747] -- 0:00:48 758000 -- (-1139.835) [-1140.303] (-1152.008) (-1142.488) * (-1150.296) [-1147.357] (-1147.725) (-1141.482) -- 0:00:48 759000 -- (-1144.208) (-1139.993) (-1144.831) [-1142.919] * (-1143.753) [-1140.975] (-1149.361) (-1148.099) -- 0:00:47 760000 -- (-1146.229) (-1142.056) [-1138.367] (-1141.749) * [-1139.141] (-1144.704) (-1147.673) (-1138.079) -- 0:00:48 Average standard deviation of split frequencies: 0.013978 761000 -- (-1141.271) (-1139.931) (-1145.791) [-1141.340] * [-1141.084] (-1142.447) (-1146.587) (-1151.616) -- 0:00:47 762000 -- [-1153.379] (-1156.611) (-1145.085) (-1146.459) * (-1139.620) [-1139.139] (-1138.911) (-1138.905) -- 0:00:47 763000 -- (-1143.034) [-1140.003] (-1144.749) (-1142.271) * (-1146.590) [-1143.708] (-1147.317) (-1142.554) -- 0:00:47 764000 -- [-1143.821] (-1146.124) (-1147.215) (-1144.858) * (-1144.371) (-1139.466) [-1140.948] (-1145.962) -- 0:00:46 765000 -- (-1143.547) (-1155.985) (-1146.108) [-1140.296] * (-1147.988) (-1145.174) [-1146.178] (-1140.132) -- 0:00:47 Average standard deviation of split frequencies: 0.013471 766000 -- (-1143.646) (-1141.326) (-1158.401) [-1147.285] * (-1157.379) (-1145.778) (-1151.138) [-1141.761] -- 0:00:46 767000 -- (-1141.209) (-1144.837) (-1142.038) [-1144.002] * (-1142.204) (-1145.320) (-1149.906) [-1143.483] -- 0:00:46 768000 -- (-1145.020) [-1152.219] (-1149.353) (-1146.495) * [-1141.947] (-1140.196) (-1145.864) (-1141.920) -- 0:00:46 769000 -- (-1146.173) (-1144.037) [-1134.712] (-1158.343) * (-1141.172) [-1141.953] (-1147.372) (-1141.050) -- 0:00:45 770000 -- [-1138.499] (-1138.979) (-1140.306) (-1146.079) * (-1148.148) (-1142.785) (-1150.015) [-1140.178] -- 0:00:46 Average standard deviation of split frequencies: 0.013253 771000 -- (-1145.442) [-1138.908] (-1149.061) (-1146.094) * (-1145.422) (-1145.114) [-1140.701] (-1137.915) -- 0:00:45 772000 -- (-1139.947) [-1136.533] (-1144.780) (-1141.098) * (-1143.573) [-1136.048] (-1144.506) (-1143.295) -- 0:00:45 773000 -- (-1140.756) [-1144.257] (-1142.611) (-1141.212) * (-1143.692) (-1142.096) [-1141.641] (-1140.609) -- 0:00:45 774000 -- (-1149.584) (-1154.553) [-1147.299] (-1148.902) * (-1146.432) [-1144.224] (-1145.951) (-1143.112) -- 0:00:44 775000 -- [-1138.898] (-1141.093) (-1150.417) (-1143.382) * (-1147.370) (-1137.642) [-1145.358] (-1147.087) -- 0:00:45 Average standard deviation of split frequencies: 0.012622 776000 -- (-1143.305) [-1141.575] (-1148.526) (-1142.392) * (-1138.295) (-1148.406) (-1144.612) [-1143.148] -- 0:00:44 777000 -- (-1147.029) (-1138.673) (-1159.027) [-1143.014] * [-1138.533] (-1147.064) (-1147.817) (-1148.255) -- 0:00:44 778000 -- (-1146.311) (-1141.255) (-1147.228) [-1142.356] * (-1139.974) (-1154.302) (-1145.644) [-1143.252] -- 0:00:44 779000 -- [-1146.742] (-1145.863) (-1141.796) (-1141.264) * (-1146.236) [-1144.295] (-1149.344) (-1145.767) -- 0:00:43 780000 -- (-1141.656) (-1142.868) [-1142.158] (-1145.864) * (-1150.651) [-1150.098] (-1148.684) (-1150.326) -- 0:00:44 Average standard deviation of split frequencies: 0.012077 781000 -- (-1145.724) [-1145.282] (-1143.734) (-1147.734) * [-1138.895] (-1144.897) (-1149.946) (-1152.361) -- 0:00:43 782000 -- (-1142.680) (-1146.818) [-1143.057] (-1144.252) * (-1144.076) [-1141.696] (-1139.151) (-1146.034) -- 0:00:43 783000 -- [-1144.585] (-1139.697) (-1145.211) (-1153.046) * (-1139.422) (-1141.069) [-1142.946] (-1155.122) -- 0:00:43 784000 -- (-1145.767) [-1139.754] (-1147.445) (-1142.515) * [-1143.139] (-1147.685) (-1147.512) (-1156.722) -- 0:00:42 785000 -- (-1143.959) [-1145.181] (-1152.363) (-1148.004) * [-1146.333] (-1148.844) (-1143.551) (-1149.529) -- 0:00:43 Average standard deviation of split frequencies: 0.012861 786000 -- (-1147.027) (-1151.296) (-1148.233) [-1141.907] * (-1149.880) [-1140.205] (-1140.763) (-1147.512) -- 0:00:42 787000 -- (-1144.174) [-1143.283] (-1156.809) (-1138.874) * (-1142.617) (-1146.143) [-1141.742] (-1147.871) -- 0:00:42 788000 -- (-1141.645) (-1145.525) [-1142.774] (-1145.643) * (-1141.715) [-1141.117] (-1142.629) (-1141.480) -- 0:00:42 789000 -- (-1151.752) (-1141.645) (-1144.665) [-1146.050] * (-1144.373) [-1146.983] (-1138.709) (-1140.284) -- 0:00:41 790000 -- (-1146.762) [-1141.466] (-1145.648) (-1138.365) * (-1143.938) (-1140.426) (-1140.091) [-1138.623] -- 0:00:42 Average standard deviation of split frequencies: 0.012719 791000 -- (-1141.643) (-1146.545) (-1140.888) [-1138.928] * [-1139.055] (-1138.906) (-1149.171) (-1142.929) -- 0:00:41 792000 -- (-1137.201) (-1145.550) (-1140.809) [-1145.263] * [-1148.651] (-1154.526) (-1143.558) (-1143.315) -- 0:00:41 793000 -- (-1146.309) [-1147.575] (-1143.893) (-1141.376) * (-1148.808) [-1143.359] (-1144.199) (-1141.739) -- 0:00:41 794000 -- (-1147.828) (-1145.607) (-1142.035) [-1145.778] * (-1143.090) (-1142.575) (-1145.092) [-1143.742] -- 0:00:40 795000 -- (-1147.612) (-1151.922) [-1151.459] (-1136.950) * (-1140.167) (-1137.399) [-1141.389] (-1144.044) -- 0:00:40 Average standard deviation of split frequencies: 0.011318 796000 -- (-1147.194) (-1141.168) [-1140.497] (-1142.269) * (-1145.872) (-1149.758) (-1146.123) [-1138.465] -- 0:00:40 797000 -- [-1147.726] (-1141.515) (-1146.151) (-1148.005) * (-1145.062) (-1148.162) (-1145.603) [-1138.679] -- 0:00:40 798000 -- (-1138.053) (-1152.581) [-1139.930] (-1148.487) * (-1140.903) (-1151.173) [-1144.913] (-1144.351) -- 0:00:40 799000 -- (-1143.946) (-1135.826) [-1143.696] (-1154.870) * [-1144.869] (-1145.299) (-1150.297) (-1144.346) -- 0:00:39 800000 -- (-1143.740) [-1140.298] (-1148.500) (-1143.232) * [-1151.098] (-1148.321) (-1144.432) (-1149.235) -- 0:00:40 Average standard deviation of split frequencies: 0.010794 801000 -- (-1144.542) [-1145.753] (-1145.318) (-1141.491) * (-1145.764) (-1144.443) [-1139.626] (-1138.896) -- 0:00:39 802000 -- (-1153.375) (-1145.207) (-1146.968) [-1141.061] * [-1141.559] (-1147.382) (-1140.677) (-1137.974) -- 0:00:39 803000 -- [-1139.871] (-1140.373) (-1149.079) (-1141.758) * (-1140.148) [-1142.767] (-1149.868) (-1140.839) -- 0:00:39 804000 -- [-1145.793] (-1145.631) (-1144.015) (-1145.599) * (-1145.383) (-1146.046) (-1144.471) [-1140.234] -- 0:00:39 805000 -- (-1142.618) (-1142.830) [-1142.369] (-1144.414) * (-1146.482) [-1140.098] (-1147.417) (-1136.679) -- 0:00:39 Average standard deviation of split frequencies: 0.010073 806000 -- (-1144.526) [-1144.297] (-1143.760) (-1146.158) * (-1145.596) (-1149.604) [-1146.673] (-1142.819) -- 0:00:38 807000 -- (-1142.892) (-1137.227) [-1144.015] (-1146.810) * (-1144.156) (-1141.381) [-1153.318] (-1155.950) -- 0:00:38 808000 -- [-1143.899] (-1144.085) (-1147.470) (-1145.504) * (-1142.635) (-1142.329) [-1139.794] (-1139.011) -- 0:00:38 809000 -- (-1147.184) [-1142.721] (-1147.784) (-1144.894) * (-1143.229) (-1136.659) (-1145.621) [-1135.865] -- 0:00:38 810000 -- (-1143.661) (-1145.250) (-1143.809) [-1140.507] * (-1152.607) [-1145.482] (-1144.406) (-1146.094) -- 0:00:38 Average standard deviation of split frequencies: 0.010725 811000 -- (-1146.244) (-1144.889) (-1143.604) [-1139.806] * (-1142.213) [-1139.290] (-1147.886) (-1148.308) -- 0:00:37 812000 -- [-1139.133] (-1140.951) (-1148.120) (-1151.630) * (-1140.497) (-1146.785) [-1141.466] (-1140.447) -- 0:00:37 813000 -- (-1140.779) (-1145.014) (-1141.791) [-1141.380] * [-1143.108] (-1146.750) (-1142.867) (-1145.189) -- 0:00:37 814000 -- [-1142.098] (-1146.760) (-1148.856) (-1141.816) * (-1145.261) (-1154.209) (-1139.746) [-1145.995] -- 0:00:37 815000 -- [-1144.388] (-1147.871) (-1150.035) (-1147.728) * (-1145.851) (-1143.363) [-1143.445] (-1151.542) -- 0:00:36 Average standard deviation of split frequencies: 0.010334 816000 -- (-1141.833) (-1162.802) [-1146.069] (-1141.818) * [-1141.417] (-1147.251) (-1142.796) (-1167.168) -- 0:00:36 817000 -- [-1137.659] (-1142.977) (-1141.812) (-1144.822) * [-1135.551] (-1145.187) (-1152.471) (-1144.090) -- 0:00:36 818000 -- [-1141.192] (-1144.683) (-1158.652) (-1142.392) * (-1144.470) [-1141.699] (-1147.109) (-1149.550) -- 0:00:36 819000 -- [-1142.213] (-1146.466) (-1144.121) (-1148.724) * (-1149.029) (-1148.442) (-1148.274) [-1143.669] -- 0:00:36 820000 -- (-1142.239) [-1139.215] (-1149.564) (-1145.618) * [-1140.490] (-1141.542) (-1137.078) (-1142.802) -- 0:00:35 Average standard deviation of split frequencies: 0.010020 821000 -- [-1141.029] (-1157.357) (-1147.706) (-1145.705) * [-1142.623] (-1144.881) (-1148.031) (-1146.248) -- 0:00:35 822000 -- (-1140.053) [-1145.740] (-1144.132) (-1139.351) * (-1146.129) [-1146.426] (-1152.521) (-1141.118) -- 0:00:35 823000 -- (-1140.319) (-1156.142) (-1145.152) [-1137.829] * [-1144.924] (-1143.196) (-1143.701) (-1138.934) -- 0:00:35 824000 -- (-1142.972) (-1140.319) (-1151.898) [-1147.994] * [-1142.833] (-1144.344) (-1138.127) (-1145.676) -- 0:00:35 825000 -- (-1141.897) (-1146.058) (-1142.033) [-1139.152] * (-1140.490) (-1142.982) (-1144.786) [-1141.370] -- 0:00:34 Average standard deviation of split frequencies: 0.010336 826000 -- (-1138.602) (-1142.669) (-1145.859) [-1147.794] * (-1142.719) [-1139.270] (-1149.343) (-1139.056) -- 0:00:34 827000 -- [-1141.034] (-1147.724) (-1142.814) (-1146.602) * (-1147.021) (-1141.154) (-1149.226) [-1140.401] -- 0:00:34 828000 -- (-1141.928) (-1153.278) [-1140.971] (-1155.840) * (-1143.875) (-1145.300) (-1143.434) [-1139.934] -- 0:00:34 829000 -- (-1143.907) (-1142.397) [-1140.122] (-1153.067) * [-1139.349] (-1139.727) (-1143.732) (-1141.872) -- 0:00:34 830000 -- (-1146.548) [-1140.162] (-1144.248) (-1149.935) * [-1146.099] (-1140.608) (-1145.460) (-1145.294) -- 0:00:33 Average standard deviation of split frequencies: 0.010215 831000 -- (-1141.288) [-1144.259] (-1146.040) (-1141.905) * (-1150.545) (-1146.501) (-1146.252) [-1139.927] -- 0:00:33 832000 -- [-1142.273] (-1148.414) (-1141.408) (-1144.982) * (-1142.749) (-1140.594) (-1142.397) [-1140.005] -- 0:00:33 833000 -- (-1139.650) (-1142.249) (-1150.023) [-1141.769] * [-1140.213] (-1144.366) (-1147.450) (-1139.576) -- 0:00:33 834000 -- (-1141.801) (-1144.337) (-1137.923) [-1136.673] * [-1144.205] (-1149.607) (-1142.253) (-1146.228) -- 0:00:33 835000 -- (-1140.580) (-1142.804) (-1142.169) [-1140.197] * (-1144.241) [-1138.222] (-1138.310) (-1145.032) -- 0:00:32 Average standard deviation of split frequencies: 0.010275 836000 -- (-1146.089) [-1146.098] (-1156.596) (-1151.353) * (-1146.686) (-1144.898) [-1137.045] (-1141.359) -- 0:00:32 837000 -- (-1152.167) (-1149.276) [-1143.366] (-1141.678) * [-1153.699] (-1147.315) (-1144.358) (-1145.925) -- 0:00:32 838000 -- (-1144.904) (-1142.974) (-1145.639) [-1140.424] * (-1141.466) (-1148.919) (-1146.970) [-1138.386] -- 0:00:32 839000 -- (-1154.773) [-1144.000] (-1140.582) (-1142.204) * [-1138.735] (-1139.960) (-1140.935) (-1138.155) -- 0:00:32 840000 -- (-1146.885) (-1153.326) (-1142.917) [-1149.025] * [-1137.876] (-1140.894) (-1142.386) (-1151.790) -- 0:00:31 Average standard deviation of split frequencies: 0.010280 841000 -- (-1147.716) (-1151.322) [-1141.846] (-1144.923) * (-1142.906) (-1145.387) (-1144.793) [-1137.972] -- 0:00:31 842000 -- (-1148.129) [-1146.111] (-1141.804) (-1146.822) * (-1145.611) (-1140.078) [-1146.327] (-1143.550) -- 0:00:31 843000 -- (-1148.605) [-1147.659] (-1144.257) (-1142.989) * [-1142.119] (-1136.803) (-1143.220) (-1148.021) -- 0:00:31 844000 -- (-1141.676) [-1145.172] (-1146.105) (-1144.268) * (-1147.600) [-1141.354] (-1150.286) (-1142.993) -- 0:00:31 845000 -- (-1147.303) [-1139.056] (-1142.651) (-1142.450) * (-1148.987) [-1136.468] (-1144.890) (-1140.974) -- 0:00:30 Average standard deviation of split frequencies: 0.009844 846000 -- [-1150.267] (-1144.647) (-1142.658) (-1138.389) * (-1157.450) (-1142.423) (-1146.100) [-1142.732] -- 0:00:30 847000 -- (-1147.396) [-1139.600] (-1140.878) (-1148.200) * [-1137.742] (-1141.402) (-1143.489) (-1141.905) -- 0:00:30 848000 -- [-1141.206] (-1141.281) (-1148.434) (-1145.656) * (-1137.100) (-1140.412) (-1145.339) [-1143.206] -- 0:00:30 849000 -- (-1141.757) (-1143.306) [-1146.109] (-1144.667) * [-1146.148] (-1145.941) (-1141.333) (-1141.719) -- 0:00:30 850000 -- (-1141.370) (-1143.348) [-1148.846] (-1141.615) * (-1138.060) (-1141.665) (-1147.630) [-1142.192] -- 0:00:29 Average standard deviation of split frequencies: 0.009852 851000 -- (-1140.707) (-1142.600) (-1141.015) [-1138.505] * [-1146.050] (-1144.331) (-1146.792) (-1145.331) -- 0:00:29 852000 -- (-1141.455) (-1143.017) (-1144.994) [-1143.804] * (-1143.513) [-1144.777] (-1146.396) (-1140.537) -- 0:00:29 853000 -- [-1142.285] (-1139.399) (-1141.562) (-1142.108) * (-1142.313) (-1140.155) [-1141.698] (-1140.578) -- 0:00:29 854000 -- (-1141.462) [-1143.241] (-1139.095) (-1142.917) * (-1152.204) [-1138.330] (-1138.910) (-1139.403) -- 0:00:29 855000 -- (-1141.685) (-1151.943) [-1137.920] (-1137.005) * (-1141.837) (-1138.722) [-1139.858] (-1142.234) -- 0:00:28 Average standard deviation of split frequencies: 0.009668 856000 -- [-1142.506] (-1140.688) (-1148.180) (-1145.513) * [-1140.527] (-1142.631) (-1138.769) (-1141.773) -- 0:00:28 857000 -- (-1142.247) (-1138.557) [-1150.081] (-1146.658) * [-1147.169] (-1143.772) (-1148.465) (-1137.385) -- 0:00:28 858000 -- [-1138.179] (-1149.425) (-1151.308) (-1142.482) * (-1153.585) [-1143.618] (-1141.933) (-1148.143) -- 0:00:28 859000 -- (-1142.400) (-1142.793) (-1149.860) [-1151.476] * (-1147.862) [-1152.757] (-1143.154) (-1143.499) -- 0:00:28 860000 -- (-1142.530) (-1141.632) (-1145.202) [-1144.746] * (-1149.244) (-1148.399) (-1146.394) [-1145.912] -- 0:00:27 Average standard deviation of split frequencies: 0.009859 861000 -- (-1154.198) (-1151.321) (-1150.859) [-1140.194] * (-1148.103) [-1145.606] (-1150.434) (-1136.192) -- 0:00:27 862000 -- (-1147.920) [-1144.353] (-1143.230) (-1143.941) * [-1144.042] (-1140.095) (-1150.637) (-1140.534) -- 0:00:27 863000 -- (-1150.268) [-1149.417] (-1149.219) (-1145.230) * (-1144.957) (-1146.275) (-1142.102) [-1145.070] -- 0:00:27 864000 -- (-1142.960) [-1144.877] (-1149.234) (-1151.018) * (-1148.386) (-1145.070) [-1143.357] (-1137.541) -- 0:00:27 865000 -- (-1148.920) (-1144.932) [-1148.740] (-1150.977) * (-1154.666) (-1142.727) [-1147.926] (-1143.225) -- 0:00:26 Average standard deviation of split frequencies: 0.009738 866000 -- (-1141.122) (-1148.831) (-1148.726) [-1145.770] * (-1145.319) (-1154.718) (-1139.342) [-1144.428] -- 0:00:26 867000 -- [-1140.095] (-1144.272) (-1143.568) (-1142.372) * (-1147.602) (-1144.967) [-1140.651] (-1143.020) -- 0:00:26 868000 -- (-1140.153) [-1148.125] (-1149.028) (-1147.438) * [-1140.804] (-1145.689) (-1146.265) (-1140.628) -- 0:00:26 869000 -- (-1146.101) (-1141.747) [-1140.921] (-1145.209) * (-1151.413) [-1140.746] (-1145.458) (-1147.688) -- 0:00:26 870000 -- (-1150.402) (-1141.312) [-1143.557] (-1143.068) * (-1151.057) (-1143.492) [-1148.240] (-1143.035) -- 0:00:25 Average standard deviation of split frequencies: 0.009686 871000 -- (-1146.932) [-1140.910] (-1144.139) (-1141.934) * (-1140.635) [-1144.875] (-1151.092) (-1144.297) -- 0:00:25 872000 -- [-1140.272] (-1144.919) (-1147.804) (-1143.549) * (-1144.832) [-1139.338] (-1141.128) (-1143.342) -- 0:00:25 873000 -- (-1140.737) [-1138.488] (-1141.900) (-1154.699) * (-1145.140) [-1142.272] (-1149.300) (-1148.809) -- 0:00:25 874000 -- (-1138.588) (-1140.692) [-1141.288] (-1144.512) * (-1147.074) [-1149.906] (-1152.731) (-1145.752) -- 0:00:25 875000 -- (-1147.864) (-1143.796) (-1143.681) [-1139.536] * [-1142.111] (-1143.463) (-1145.929) (-1141.357) -- 0:00:24 Average standard deviation of split frequencies: 0.009866 876000 -- (-1145.152) (-1147.264) (-1147.595) [-1149.315] * (-1145.298) [-1138.155] (-1142.903) (-1159.111) -- 0:00:24 877000 -- [-1143.927] (-1138.935) (-1134.478) (-1153.525) * (-1140.395) (-1147.140) [-1143.650] (-1147.227) -- 0:00:24 878000 -- (-1149.445) [-1145.027] (-1149.505) (-1142.988) * (-1144.100) [-1140.916] (-1141.145) (-1147.655) -- 0:00:24 879000 -- [-1142.324] (-1141.508) (-1148.949) (-1140.705) * (-1143.113) (-1154.570) (-1141.991) [-1137.444] -- 0:00:24 880000 -- (-1140.849) [-1139.160] (-1143.952) (-1145.062) * [-1139.533] (-1143.495) (-1139.366) (-1141.915) -- 0:00:23 Average standard deviation of split frequencies: 0.009754 881000 -- (-1141.207) (-1153.623) [-1141.259] (-1145.445) * (-1149.134) (-1153.814) [-1150.755] (-1150.644) -- 0:00:23 882000 -- (-1139.520) [-1140.755] (-1142.026) (-1148.295) * (-1144.868) (-1144.303) (-1147.813) [-1139.360] -- 0:00:23 883000 -- (-1141.953) (-1143.987) (-1143.961) [-1148.680] * (-1139.551) [-1141.369] (-1143.011) (-1141.173) -- 0:00:23 884000 -- [-1140.277] (-1149.210) (-1149.363) (-1141.821) * (-1145.832) [-1140.874] (-1154.799) (-1141.226) -- 0:00:23 885000 -- (-1144.636) (-1143.498) (-1143.609) [-1143.312] * (-1141.582) [-1138.345] (-1143.349) (-1146.884) -- 0:00:22 Average standard deviation of split frequencies: 0.010346 886000 -- (-1145.606) [-1146.420] (-1140.526) (-1151.895) * [-1139.984] (-1142.667) (-1145.480) (-1141.607) -- 0:00:22 887000 -- (-1142.585) [-1142.638] (-1144.851) (-1149.548) * (-1138.021) (-1141.737) [-1145.499] (-1148.232) -- 0:00:22 888000 -- (-1140.321) [-1140.936] (-1153.062) (-1139.493) * (-1140.594) [-1141.405] (-1143.838) (-1145.342) -- 0:00:22 889000 -- [-1139.161] (-1145.820) (-1145.597) (-1141.780) * (-1136.646) [-1143.433] (-1147.912) (-1143.465) -- 0:00:22 890000 -- [-1139.819] (-1142.715) (-1144.720) (-1146.131) * (-1143.451) (-1143.864) (-1148.992) [-1141.267] -- 0:00:21 Average standard deviation of split frequencies: 0.009762 891000 -- (-1139.159) [-1149.123] (-1144.876) (-1139.849) * (-1147.003) (-1147.452) (-1153.896) [-1139.512] -- 0:00:21 892000 -- (-1144.456) (-1138.371) (-1144.667) [-1140.377] * (-1152.934) (-1148.654) (-1148.611) [-1138.536] -- 0:00:21 893000 -- (-1152.864) [-1143.237] (-1142.587) (-1148.575) * (-1146.744) (-1139.499) (-1147.130) [-1138.559] -- 0:00:21 894000 -- (-1143.054) (-1143.698) [-1141.142] (-1135.599) * (-1139.528) (-1154.324) (-1138.444) [-1144.341] -- 0:00:21 895000 -- [-1147.645] (-1147.264) (-1155.406) (-1142.748) * (-1146.427) [-1148.717] (-1143.917) (-1139.437) -- 0:00:20 Average standard deviation of split frequencies: 0.010172 896000 -- [-1136.651] (-1144.075) (-1140.751) (-1149.148) * (-1146.787) (-1157.947) (-1147.516) [-1140.010] -- 0:00:20 897000 -- (-1143.467) (-1144.573) (-1147.808) [-1140.369] * (-1143.206) (-1145.880) [-1144.275] (-1147.740) -- 0:00:20 898000 -- (-1142.544) [-1140.453] (-1146.991) (-1141.337) * (-1144.059) (-1141.962) (-1150.239) [-1137.443] -- 0:00:20 899000 -- (-1141.751) [-1141.884] (-1146.205) (-1139.089) * (-1144.766) (-1146.305) (-1142.999) [-1143.685] -- 0:00:20 900000 -- [-1143.557] (-1142.617) (-1142.751) (-1140.427) * (-1143.115) [-1139.756] (-1141.084) (-1147.600) -- 0:00:19 Average standard deviation of split frequencies: 0.010177 901000 -- (-1137.535) (-1138.710) [-1145.816] (-1140.359) * [-1137.934] (-1140.276) (-1145.004) (-1145.258) -- 0:00:19 902000 -- (-1149.167) [-1138.007] (-1141.925) (-1144.546) * (-1139.319) (-1142.634) [-1137.942] (-1141.046) -- 0:00:19 903000 -- (-1150.909) (-1139.664) [-1140.682] (-1145.060) * (-1140.397) [-1146.388] (-1147.650) (-1143.703) -- 0:00:19 904000 -- [-1140.326] (-1141.539) (-1144.969) (-1142.647) * (-1155.243) (-1143.490) [-1142.106] (-1138.786) -- 0:00:19 905000 -- [-1138.976] (-1143.916) (-1140.810) (-1149.609) * (-1143.012) [-1152.915] (-1145.059) (-1140.771) -- 0:00:18 Average standard deviation of split frequencies: 0.009423 906000 -- (-1146.201) (-1140.866) (-1142.161) [-1141.992] * [-1143.761] (-1144.122) (-1140.784) (-1144.892) -- 0:00:18 907000 -- (-1149.062) [-1143.212] (-1142.727) (-1140.691) * [-1146.465] (-1146.157) (-1149.771) (-1141.299) -- 0:00:18 908000 -- (-1139.810) (-1141.206) [-1136.055] (-1148.237) * [-1143.694] (-1143.214) (-1156.550) (-1150.090) -- 0:00:18 909000 -- [-1139.628] (-1144.184) (-1142.438) (-1150.361) * (-1145.497) (-1143.362) [-1140.759] (-1149.991) -- 0:00:18 910000 -- (-1147.006) (-1146.783) [-1143.156] (-1143.093) * [-1144.112] (-1139.355) (-1145.479) (-1143.895) -- 0:00:17 Average standard deviation of split frequencies: 0.009145 911000 -- (-1145.367) (-1145.374) (-1142.237) [-1142.490] * (-1147.979) (-1157.002) (-1146.851) [-1144.100] -- 0:00:17 912000 -- (-1140.960) (-1145.301) [-1142.134] (-1141.947) * (-1147.515) (-1150.229) [-1142.730] (-1149.696) -- 0:00:17 913000 -- [-1140.544] (-1146.619) (-1144.818) (-1145.184) * (-1144.403) (-1141.901) (-1150.351) [-1140.833] -- 0:00:17 914000 -- (-1154.975) (-1142.682) [-1148.964] (-1148.587) * [-1144.563] (-1143.104) (-1145.535) (-1136.345) -- 0:00:17 915000 -- [-1142.616] (-1140.441) (-1149.661) (-1144.063) * (-1141.076) (-1147.082) (-1147.833) [-1137.210] -- 0:00:16 Average standard deviation of split frequencies: 0.009092 916000 -- (-1147.008) [-1143.340] (-1143.408) (-1145.639) * [-1141.039] (-1143.192) (-1138.707) (-1136.689) -- 0:00:16 917000 -- (-1146.837) [-1142.451] (-1146.752) (-1145.888) * (-1149.874) (-1143.616) (-1157.516) [-1139.980] -- 0:00:16 918000 -- (-1146.736) (-1145.819) (-1149.731) [-1139.884] * (-1144.735) (-1139.169) [-1139.720] (-1144.486) -- 0:00:16 919000 -- (-1141.454) (-1139.215) [-1138.919] (-1141.908) * (-1148.384) (-1137.380) [-1143.917] (-1151.841) -- 0:00:16 920000 -- (-1137.073) (-1147.106) (-1146.641) [-1145.666] * (-1145.066) [-1141.571] (-1147.741) (-1139.384) -- 0:00:15 Average standard deviation of split frequencies: 0.008249 921000 -- [-1143.224] (-1138.532) (-1141.039) (-1147.167) * (-1143.926) (-1143.759) (-1151.720) [-1145.879] -- 0:00:15 922000 -- [-1139.292] (-1144.658) (-1145.636) (-1144.599) * (-1144.994) [-1142.454] (-1145.870) (-1145.066) -- 0:00:15 923000 -- (-1142.462) (-1148.115) [-1143.597] (-1139.988) * (-1143.247) (-1145.060) [-1143.656] (-1137.203) -- 0:00:15 924000 -- (-1145.205) [-1140.292] (-1139.580) (-1145.596) * (-1145.656) [-1149.105] (-1148.002) (-1141.407) -- 0:00:15 925000 -- (-1139.345) (-1141.053) [-1145.813] (-1144.302) * (-1144.596) [-1141.541] (-1142.704) (-1141.568) -- 0:00:14 Average standard deviation of split frequencies: 0.007976 926000 -- (-1143.666) (-1149.533) (-1141.514) [-1140.355] * (-1147.038) [-1143.629] (-1144.683) (-1142.042) -- 0:00:14 927000 -- (-1143.809) (-1149.085) [-1143.532] (-1138.418) * (-1143.259) [-1142.187] (-1156.400) (-1146.936) -- 0:00:14 928000 -- (-1142.373) (-1145.165) (-1142.171) [-1141.958] * (-1142.206) (-1138.563) (-1146.359) [-1145.492] -- 0:00:14 929000 -- (-1145.620) (-1152.232) [-1142.454] (-1142.516) * (-1157.207) (-1142.203) (-1142.680) [-1141.286] -- 0:00:14 930000 -- [-1145.723] (-1145.099) (-1148.078) (-1142.607) * (-1148.785) (-1145.362) [-1140.535] (-1140.430) -- 0:00:13 Average standard deviation of split frequencies: 0.007936 931000 -- [-1143.741] (-1146.852) (-1141.565) (-1148.196) * (-1145.348) (-1142.816) (-1143.149) [-1145.802] -- 0:00:13 932000 -- (-1143.700) [-1140.081] (-1144.801) (-1138.059) * (-1145.090) (-1149.058) (-1150.843) [-1141.754] -- 0:00:13 933000 -- (-1144.348) (-1149.232) (-1141.033) [-1142.420] * (-1149.249) (-1144.008) (-1146.729) [-1143.303] -- 0:00:13 934000 -- (-1142.526) (-1149.565) [-1143.261] (-1153.973) * (-1142.268) (-1146.895) (-1144.246) [-1140.252] -- 0:00:13 935000 -- (-1145.282) (-1147.299) (-1144.333) [-1141.765] * (-1142.293) (-1147.603) [-1140.332] (-1142.167) -- 0:00:12 Average standard deviation of split frequencies: 0.007778 936000 -- (-1143.626) [-1144.125] (-1141.624) (-1141.784) * (-1142.340) [-1144.518] (-1142.552) (-1142.076) -- 0:00:12 937000 -- (-1148.235) (-1145.967) [-1145.764] (-1146.881) * (-1148.782) [-1142.252] (-1146.867) (-1146.511) -- 0:00:12 938000 -- [-1143.305] (-1142.416) (-1144.858) (-1138.820) * (-1139.236) [-1143.183] (-1145.666) (-1146.011) -- 0:00:12 939000 -- (-1148.931) (-1150.119) [-1150.943] (-1143.380) * [-1146.437] (-1143.189) (-1143.717) (-1140.892) -- 0:00:12 940000 -- [-1144.496] (-1142.309) (-1146.972) (-1140.141) * [-1141.936] (-1145.191) (-1148.083) (-1150.115) -- 0:00:11 Average standard deviation of split frequencies: 0.007573 941000 -- (-1140.981) [-1139.363] (-1142.762) (-1144.433) * (-1138.705) (-1143.629) (-1147.954) [-1141.774] -- 0:00:11 942000 -- [-1140.761] (-1141.362) (-1144.912) (-1143.934) * [-1137.755] (-1143.719) (-1150.222) (-1140.810) -- 0:00:11 943000 -- (-1146.404) (-1140.440) (-1149.193) [-1144.542] * [-1147.903] (-1147.747) (-1149.126) (-1139.307) -- 0:00:11 944000 -- (-1144.059) [-1143.126] (-1145.376) (-1140.853) * [-1138.032] (-1147.795) (-1146.387) (-1146.350) -- 0:00:11 945000 -- (-1144.661) [-1144.958] (-1144.535) (-1149.937) * [-1146.118] (-1148.236) (-1138.701) (-1139.772) -- 0:00:10 Average standard deviation of split frequencies: 0.007419 946000 -- (-1142.753) (-1147.510) (-1145.896) [-1145.719] * (-1146.085) [-1146.002] (-1141.705) (-1140.949) -- 0:00:10 947000 -- [-1142.508] (-1144.245) (-1147.765) (-1147.516) * (-1145.633) (-1150.638) [-1144.388] (-1150.884) -- 0:00:10 948000 -- (-1139.638) (-1142.678) [-1144.407] (-1145.033) * (-1144.130) (-1142.815) [-1142.371] (-1142.087) -- 0:00:10 949000 -- [-1140.645] (-1149.474) (-1141.808) (-1141.899) * [-1142.157] (-1145.576) (-1141.064) (-1149.401) -- 0:00:10 950000 -- (-1142.633) [-1140.858] (-1141.247) (-1144.474) * (-1145.104) (-1151.907) (-1138.565) [-1144.483] -- 0:00:09 Average standard deviation of split frequencies: 0.007658 951000 -- (-1142.530) [-1137.336] (-1148.126) (-1143.250) * (-1145.027) (-1146.036) [-1141.637] (-1147.772) -- 0:00:09 952000 -- (-1143.025) (-1154.838) (-1137.899) [-1147.174] * (-1141.288) (-1156.338) (-1149.115) [-1140.725] -- 0:00:09 953000 -- (-1145.764) [-1137.621] (-1144.772) (-1142.273) * (-1140.317) [-1138.299] (-1146.319) (-1150.395) -- 0:00:09 954000 -- (-1141.842) (-1142.587) [-1147.897] (-1142.750) * (-1144.117) (-1145.692) [-1142.833] (-1152.600) -- 0:00:09 955000 -- (-1144.079) [-1140.840] (-1148.538) (-1145.989) * (-1143.646) [-1140.207] (-1139.883) (-1148.684) -- 0:00:08 Average standard deviation of split frequencies: 0.007835 956000 -- (-1142.913) [-1144.509] (-1140.857) (-1143.851) * (-1146.621) (-1142.431) [-1139.920] (-1141.806) -- 0:00:08 957000 -- [-1141.228] (-1149.385) (-1147.540) (-1146.007) * (-1145.467) (-1138.322) (-1152.131) [-1145.549] -- 0:00:08 958000 -- (-1141.129) [-1144.278] (-1141.679) (-1141.573) * (-1141.002) (-1145.315) (-1145.253) [-1139.173] -- 0:00:08 959000 -- (-1143.194) [-1142.669] (-1142.802) (-1147.807) * (-1147.399) (-1148.271) (-1141.858) [-1152.134] -- 0:00:08 960000 -- (-1141.160) [-1143.036] (-1141.666) (-1141.899) * (-1146.140) (-1150.643) [-1139.259] (-1144.886) -- 0:00:07 Average standard deviation of split frequencies: 0.008015 961000 -- (-1144.739) [-1140.369] (-1147.889) (-1144.676) * (-1146.341) (-1149.145) [-1145.356] (-1147.838) -- 0:00:07 962000 -- (-1151.634) (-1143.523) [-1141.697] (-1139.148) * (-1149.383) (-1142.509) [-1141.968] (-1139.544) -- 0:00:07 963000 -- (-1143.242) (-1142.238) (-1145.946) [-1140.354] * (-1140.580) (-1151.719) (-1140.900) [-1140.101] -- 0:00:07 964000 -- [-1139.280] (-1153.406) (-1142.097) (-1149.404) * (-1148.370) [-1142.424] (-1147.218) (-1146.618) -- 0:00:07 965000 -- [-1139.331] (-1143.487) (-1138.312) (-1143.103) * (-1140.016) (-1143.064) [-1140.450] (-1142.657) -- 0:00:06 Average standard deviation of split frequencies: 0.007483 966000 -- (-1145.724) (-1152.304) (-1140.923) [-1143.914] * [-1139.395] (-1146.342) (-1142.936) (-1141.517) -- 0:00:06 967000 -- (-1143.021) (-1153.466) [-1137.081] (-1145.155) * (-1147.453) (-1141.400) (-1150.203) [-1135.932] -- 0:00:06 968000 -- (-1145.751) (-1146.089) [-1141.079] (-1146.413) * [-1144.096] (-1141.785) (-1145.730) (-1138.126) -- 0:00:06 969000 -- (-1155.005) (-1138.231) (-1142.940) [-1146.846] * [-1141.658] (-1147.238) (-1147.765) (-1139.215) -- 0:00:06 970000 -- (-1146.982) (-1141.282) [-1138.301] (-1140.914) * (-1139.798) [-1143.299] (-1145.017) (-1144.115) -- 0:00:05 Average standard deviation of split frequencies: 0.007285 971000 -- (-1144.469) [-1139.517] (-1141.242) (-1136.455) * (-1143.565) (-1147.778) (-1144.944) [-1144.351] -- 0:00:05 972000 -- (-1153.309) (-1144.011) [-1138.969] (-1140.021) * (-1139.915) (-1143.102) [-1139.134] (-1138.301) -- 0:00:05 973000 -- [-1138.798] (-1146.952) (-1147.687) (-1143.943) * (-1144.106) (-1139.648) [-1142.400] (-1149.857) -- 0:00:05 974000 -- (-1149.447) (-1139.369) (-1148.941) [-1143.740] * (-1148.775) [-1139.662] (-1149.496) (-1150.860) -- 0:00:05 975000 -- (-1135.950) (-1148.798) [-1138.234] (-1137.923) * [-1146.436] (-1144.938) (-1140.962) (-1147.648) -- 0:00:04 Average standard deviation of split frequencies: 0.007030 976000 -- (-1145.757) (-1142.413) [-1140.518] (-1151.071) * (-1144.510) (-1140.099) (-1155.195) [-1143.208] -- 0:00:04 977000 -- (-1145.335) (-1141.170) [-1138.500] (-1145.023) * [-1137.737] (-1142.849) (-1150.922) (-1143.965) -- 0:00:04 978000 -- (-1146.978) [-1137.821] (-1142.070) (-1144.670) * (-1156.125) (-1145.593) (-1138.717) [-1148.258] -- 0:00:04 979000 -- (-1144.978) (-1149.149) [-1146.585] (-1144.776) * (-1145.210) (-1150.101) [-1146.376] (-1140.517) -- 0:00:04 980000 -- [-1146.549] (-1144.905) (-1144.482) (-1141.248) * (-1148.159) (-1145.047) [-1142.647] (-1146.032) -- 0:00:03 Average standard deviation of split frequencies: 0.006997 981000 -- (-1153.058) [-1134.945] (-1142.234) (-1145.167) * (-1143.605) (-1149.314) (-1143.570) [-1140.259] -- 0:00:03 982000 -- (-1150.214) (-1141.648) [-1148.340] (-1143.334) * (-1145.903) (-1148.859) (-1147.947) [-1136.097] -- 0:00:03 983000 -- (-1144.243) [-1138.511] (-1147.029) (-1144.153) * (-1141.857) (-1149.110) [-1141.909] (-1138.189) -- 0:00:03 984000 -- (-1141.666) (-1144.833) (-1144.588) [-1141.630] * (-1147.726) [-1146.662] (-1142.256) (-1143.785) -- 0:00:03 985000 -- (-1140.415) (-1137.362) [-1147.428] (-1153.564) * (-1149.714) (-1147.448) [-1142.644] (-1147.503) -- 0:00:02 Average standard deviation of split frequencies: 0.006534 986000 -- (-1143.133) (-1144.274) [-1143.281] (-1147.867) * (-1143.496) (-1146.093) (-1146.316) [-1144.526] -- 0:00:02 987000 -- (-1141.650) (-1144.922) [-1142.121] (-1151.928) * (-1147.787) (-1146.504) (-1149.903) [-1143.085] -- 0:00:02 988000 -- (-1140.625) (-1143.562) (-1143.488) [-1143.583] * (-1146.908) [-1147.902] (-1141.222) (-1144.851) -- 0:00:02 989000 -- [-1146.972] (-1144.926) (-1141.999) (-1147.968) * (-1142.438) (-1145.212) (-1140.459) [-1143.339] -- 0:00:02 990000 -- (-1147.270) (-1148.391) [-1147.183] (-1142.884) * (-1149.253) [-1142.252] (-1143.839) (-1151.843) -- 0:00:01 Average standard deviation of split frequencies: 0.006609 991000 -- (-1142.366) (-1152.062) (-1154.833) [-1138.834] * (-1146.999) (-1148.414) [-1139.130] (-1147.575) -- 0:00:01 992000 -- [-1143.688] (-1141.344) (-1146.522) (-1138.132) * (-1146.134) (-1148.782) [-1143.033] (-1148.403) -- 0:00:01 993000 -- (-1147.969) [-1139.566] (-1142.642) (-1146.680) * [-1145.649] (-1149.295) (-1145.389) (-1149.827) -- 0:00:01 994000 -- (-1145.491) (-1151.358) (-1147.205) [-1141.098] * [-1139.616] (-1144.166) (-1143.358) (-1150.253) -- 0:00:01 995000 -- (-1141.259) (-1155.694) [-1138.040] (-1146.400) * (-1144.687) (-1140.518) (-1147.480) [-1144.277] -- 0:00:00 Average standard deviation of split frequencies: 0.006731 996000 -- (-1147.806) [-1136.233] (-1135.693) (-1144.097) * [-1142.735] (-1140.115) (-1147.213) (-1147.704) -- 0:00:00 997000 -- (-1139.319) [-1143.430] (-1141.127) (-1148.088) * (-1139.804) (-1144.733) (-1136.980) [-1145.354] -- 0:00:00 998000 -- (-1146.736) [-1143.970] (-1143.247) (-1149.638) * (-1140.417) [-1149.175] (-1144.243) (-1145.420) -- 0:00:00 999000 -- (-1143.254) [-1143.793] (-1146.441) (-1137.892) * (-1143.911) (-1144.950) (-1139.115) [-1140.422] -- 0:00:00 1000000 -- [-1142.338] (-1152.537) (-1143.406) (-1138.713) * (-1147.840) (-1142.231) (-1147.584) [-1143.177] -- 0:00:00 Average standard deviation of split frequencies: 0.006386 Analysis completed in 3 mins 19 seconds Analysis used 197.13 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -1132.56 Likelihood of best state for "cold" chain of run 2 was -1132.70 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 68.1 % ( 70 %) Dirichlet(Revmat{all}) 82.6 % ( 71 %) Slider(Revmat{all}) 28.1 % ( 23 %) Dirichlet(Pi{all}) 30.7 % ( 29 %) Slider(Pi{all}) 79.7 % ( 60 %) Multiplier(Alpha{1,2}) 74.2 % ( 57 %) Multiplier(Alpha{3}) 92.4 % ( 80 %) Slider(Pinvar{all}) 38.8 % ( 34 %) ExtSPR(Tau{all},V{all}) 10.5 % ( 11 %) ExtTBR(Tau{all},V{all}) 40.7 % ( 34 %) NNI(Tau{all},V{all}) 40.6 % ( 57 %) ParsSPR(Tau{all},V{all}) 27.3 % ( 27 %) Multiplier(V{all}) 55.5 % ( 59 %) Nodeslider(V{all}) 26.7 % ( 28 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 68.1 % ( 66 %) Dirichlet(Revmat{all}) 82.7 % ( 73 %) Slider(Revmat{all}) 28.0 % ( 29 %) Dirichlet(Pi{all}) 30.7 % ( 27 %) Slider(Pi{all}) 79.6 % ( 64 %) Multiplier(Alpha{1,2}) 74.3 % ( 56 %) Multiplier(Alpha{3}) 92.0 % ( 83 %) Slider(Pinvar{all}) 38.7 % ( 34 %) ExtSPR(Tau{all},V{all}) 10.5 % ( 10 %) ExtTBR(Tau{all},V{all}) 41.1 % ( 46 %) NNI(Tau{all},V{all}) 40.6 % ( 41 %) ParsSPR(Tau{all},V{all}) 27.3 % ( 23 %) Multiplier(V{all}) 55.1 % ( 57 %) Nodeslider(V{all}) 26.1 % ( 15 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.77 0.57 0.41 2 | 166961 0.79 0.60 3 | 166872 166587 0.80 4 | 166696 166777 166107 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.77 0.57 0.42 2 | 166687 0.79 0.60 3 | 166630 166670 0.80 4 | 166549 167154 166310 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -1141.03 | 1 2 | | 2 1 | | 2 1 2 2 2 | | 1 1 2 1 | | 1 2 12 22 21 | | 1* 2 1 2 212 1 1 2 1| |1 21 2 2 1 2 2 1 1 | | 1 12 2 1 2 2 12 1 2 | | 1 1 * 2 2 122 1 1 2 | | 21 * 11 2122221 2 2 1 1 1 | | 22 2 1 1 1 1 1 2 2 2 2 | | 2 2 2 1 1 1 1 1 1 | | * 1 * 1 2 2| |2 12 | | 1 1 1 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -1144.23 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -1139.12 -1150.42 2 -1139.29 -1149.83 -------------------------------------- TOTAL -1139.20 -1150.16 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.049578 0.000102 0.030295 0.068721 0.048715 1414.79 1457.89 1.000 r(A<->C){all} 0.061237 0.001914 0.000028 0.142159 0.052211 496.61 499.39 1.000 r(A<->G){all} 0.306110 0.006913 0.134887 0.457028 0.301729 375.43 572.28 1.001 r(A<->T){all} 0.059644 0.001250 0.006662 0.133816 0.052875 645.68 701.47 1.000 r(C<->G){all} 0.069321 0.002295 0.000017 0.165810 0.060207 457.72 517.98 1.000 r(C<->T){all} 0.430848 0.007388 0.272293 0.607699 0.428324 505.73 552.14 1.000 r(G<->T){all} 0.072841 0.001740 0.007487 0.156303 0.065528 709.41 770.37 1.000 pi(A){all} 0.248685 0.000264 0.217335 0.279707 0.248352 1313.46 1371.38 1.000 pi(C){all} 0.206265 0.000224 0.174630 0.233635 0.206093 942.43 1086.87 1.000 pi(G){all} 0.210617 0.000239 0.180093 0.241056 0.210350 874.14 1020.64 1.000 pi(T){all} 0.334432 0.000316 0.298087 0.367646 0.334272 1084.71 1197.33 1.000 alpha{1,2} 0.953913 0.998593 0.000152 2.904780 0.649124 1223.53 1233.29 1.000 alpha{3} 1.395424 1.218042 0.000103 3.552084 1.115954 1379.38 1400.87 1.000 pinvar{all} 0.306850 0.041346 0.000125 0.674594 0.284014 687.06 732.45 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C2 3 -- C3 4 -- C4 5 -- C5 6 -- C6 7 -- C7 8 -- C8 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition -------------- 1 -- .******* 2 -- .*...... 3 -- ..*..... 4 -- ...*.... 5 -- ....*... 6 -- .....*.. 7 -- ......*. 8 -- .......* 9 -- ..****.. 10 -- .*****.. 11 -- ...***.. 12 -- ......** 13 -- ....**.. 14 -- ...**... 15 -- .*****.* 16 -- ...*.*.. 17 -- .******. -------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 9 3002 1.000000 0.000000 1.000000 1.000000 2 10 2998 0.998668 0.000000 0.998668 0.998668 2 11 2887 0.961692 0.000471 0.961359 0.962025 2 12 1051 0.350100 0.010835 0.342438 0.357761 2 13 986 0.328448 0.000942 0.327781 0.329114 2 14 982 0.327115 0.020728 0.312458 0.341772 2 15 982 0.327115 0.010364 0.319787 0.334444 2 16 970 0.323118 0.013191 0.313791 0.332445 2 17 968 0.322452 0.000942 0.321785 0.323118 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.001112 0.000001 0.000000 0.003434 0.000751 1.000 2 length{all}[2] 0.001126 0.000001 0.000000 0.003398 0.000763 1.000 2 length{all}[3] 0.011776 0.000019 0.004546 0.020201 0.011082 1.000 2 length{all}[4] 0.001126 0.000001 0.000000 0.003499 0.000756 1.000 2 length{all}[5] 0.001074 0.000001 0.000000 0.003260 0.000720 1.000 2 length{all}[6] 0.001102 0.000001 0.000000 0.003348 0.000759 1.000 2 length{all}[7] 0.005554 0.000007 0.001501 0.010863 0.005117 1.000 2 length{all}[8] 0.002189 0.000002 0.000088 0.005317 0.001809 1.000 2 length{all}[9] 0.014929 0.000023 0.006528 0.024601 0.014385 1.000 2 length{all}[10] 0.003263 0.000004 0.000393 0.007209 0.002871 1.000 2 length{all}[11] 0.004170 0.000005 0.000205 0.008800 0.003738 1.000 2 length{all}[12] 0.001125 0.000001 0.000000 0.003259 0.000767 1.000 2 length{all}[13] 0.001141 0.000001 0.000002 0.003457 0.000783 0.999 2 length{all}[14] 0.001160 0.000001 0.000001 0.003346 0.000803 0.999 2 length{all}[15] 0.001187 0.000001 0.000002 0.003636 0.000806 0.999 2 length{all}[16] 0.001101 0.000001 0.000003 0.003266 0.000743 1.000 2 length{all}[17] 0.001104 0.000001 0.000000 0.003451 0.000775 0.999 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.006386 Maximum standard deviation of split frequencies = 0.020728 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.000 Clade credibility values: /------------------------------------------------------------------------ C1 (1) | |------------------------------------------------------------------------ C7 (7) | +------------------------------------------------------------------------ C8 (8) | | /------------------------------------------------------ C2 (2) | | \-------100-------+ /------------------------------------ C3 (3) | | \-------100-------+ /------------------ C4 (4) | | \--------96-------+------------------ C5 (5) | \------------------ C6 (6) Phylogram (based on average branch lengths): /-- C1 (1) | |------------- C7 (7) | +----- C8 (8) | | /-- C2 (2) | | \------+ /---------------------------- C3 (3) | | \------------------------------------+ /-- C4 (4) | | \--------+-- C5 (5) | \-- C6 (6) |-----------| 0.005 expected changes per site Calculating tree probabilities... Credible sets of trees (48 trees sampled): 50 % credible set contains 5 trees 90 % credible set contains 9 trees 95 % credible set contains 9 trees 99 % credible set contains 29 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C7,C8,(C2,(C3,(C4,C5,C6))))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **8** sequences, **229** codons, and **1** partitions from `/data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/results/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/results/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/results/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -1129.77, AIC-c = 2297.68 (19 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.046 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 0.643 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 2 | 1 | 3.550 | 27.257 | Pos. posterior = 0.9221 | | 4 | 1 | 3.051 | 31.224 | Pos. posterior = 0.9460 | | 7 | 1 | 3.564 | 27.662 | Pos. posterior = 0.9232 | ---- ## FUBAR inferred 3 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.21 are expected to be false positives (95% confidence interval of 0-1 )
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.08 sec, SCORE=1000, Nseq=8, Len=229 CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 MSTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSSNSTESVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 MMTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV :.*.::****************************************** CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 EVLIMWLLWLLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII ::******* **************************************** CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRFYSIPMPVAPTGITLTILSG Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG ****************************** ******************* CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF ************************************************** CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 YVRSKHGDYSALSNSSDNLTENDRLLHLV *****************************
>CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 ---ATGACTGATTCTAACAACACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGCTGGAATATCATACTGACAGTATTTATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTATGGAGTTAAGATGTTAATCATGTGGCTCCTATGGCCCTTAGTCATTGCTCTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTTAATTGGTGGTTCTTCTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGATCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATTTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 ATGAGTACTGATTCTAACAACACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGCTGGAATATCATACTGACAGTATTTATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTATGGAGTTAAGATGTTAATCATGTGGCTCCTATGGCCCTTAGTCATTGCTCTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTTAATTGGTGGTTCTTCTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGATCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATTTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 ATGAGTTCTAATTCTACTGAGTCGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGGAACTGGAACTTCAGTTGGAATATTATACTGACAGTATTCATTGCAGTGCTTCAATATGGTAATATGAAGTACAGTTTCTTCCTCTATGGAGTTAAGATGTTGATCATGTGGCTTTTATGGCCCTTAGTCATTGCTTTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTCAATTGGTGGTTCTTCTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGGTCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATCTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 ATGAGTACTAGTTCTAATGAGACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGTTGGAATATCATACTGACAGTATTCATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTACGGAGTTAAGATGTTAATCATGTGGCTTTTATGGCCCTTAGTCATTGCTTTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTCAATTGGTGGTTCTTTTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGGTCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATCTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 ATGAGTACTAGTTCTAATGAGACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGTTGGAATATCATACTGACAGTATTCATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTACGGAGTTAAGATGTTAATCATGTGGCTTTTATGGCCCTTAGTCATTGCTTTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTCAATTGGTGGTTCTTTTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGGTCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATCTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 ATGAGTACTAGTTCTAATGAGACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGTTGGAATATCATACTGACAGTATTCATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTACGGAGTTAAGATGTTAATCATGTGGCTTTTATGGCCCTTAGTCATTGCTTTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTCAATTGGTGGTTCTTTTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGGTCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATCTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 ATGATGACTGATTCTAACAACACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGCTGGAATATCATACTGACAGTATTTATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTATGGAGTTGAGGTGTTAATCATGTGGCTCCTATGGCTCTTAGTCATTGCTCTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTTAATTGGTGGTTCTTCTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGATTCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATTTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC >Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 ---ATGACTGATTCTAACAACACGGTCCCTGTGACCGAAGTTTTAGAACATCTTAGAAACTGGAACTTCAGCTGGAATATCATACTGACAGTATTTATTGCAGTGCTTCAGTATGGTAACATGAAGTACAGTTTCTTTCTCTATGGAGTTAAGATGTTAATCATGTGGCTCCTATGGCCCCTAGTCATTGCTCTGAGCATTTTTAATGCCTATGCTGACTTTGGTGTTAATTGGTGGTTCTTCTCCTTCAGCATTCTGATGCTTGTCATAACGCTAGTTCTCTGGCTTATGTACATAATTAATAGTTTTAAGCTTTATCGCAGAACGCGAACGTTTTGGGCATTTAACCCCGAAACAGACGCCATTGCTGTCATTTCAGTCTTTGGTAGATCCTACTCGATACCTATGCCAGTGGCACCAACAGGTATTACTCTGACGATTTTAAGTGGAACACTCTTTTTCGATGGCATCAGAATTGCTACTGGTGTGCAGCCTGCACACTTACCGCAATTTGTGACTGTCGCCAAACCTGGCACGACTATAATTTATACTCGTGCGGGGCGTTCCCTTAATGCAAGCACCAACACTGGTTGGGCTTTTTATGTCAGAAGTAAGCATGGTGACTATTCTGCTTTATCAAATAGTAGTGATAACTTGACAGAAAATGACAGATTGCTGCATTTAGTC
>CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 MSTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSSNSTESVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 MSTSSNETVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 MMTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV EVLIMWLLWLLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRFYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV >Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 -MTDSNNTVPVTEVLEHLRNWNFSWNIILTVFIAVLQYGNMKYSFFLYGV KMLIMWLLWPLVIALSIFNAYADFGVNWWFFSFSILMLVITLVLWLMYII NSFKLYRRTRTFWAFNPETDAIAVISVFGRSYSIPMPVAPTGITLTILSG TLFFDGIRIATGVQPAHLPQFVTVAKPGTTIIYTRAGRSLNASTNTGWAF YVRSKHGDYSALSNSSDNLTENDRLLHLV
Reading sequence file /data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/fasta/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1 Found 8 sequences of length 687 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 1.9% Found 17 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 2 Calculating analytical mean and variance Doing permutation test for PHI Doing permutation test for NSS Doing Permutation test for MAXCHI Writing alignment of polymorphic unambig sites to: Phi.poly.sites Window size is 20 polymorphic sites **p-Value(s)** ---------- NSS: 1.00e+00 (1000 permutations) Max Chi^2: 3.56e-01 (1000 permutations) PHI (Permutation): 1.00e+00 (1000 permutations) PHI (Normal): 1.00e+00
#NEXUS [ID: 8650669575] begin taxa; dimensions ntax=8; taxlabels CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2 GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2 HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2 PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2 Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 ; end; begin trees; translate 1 CHN_GD_2017_M_QAS69031_1_2017_07_China_Swine_Rhinolophus_bat_coronavirus_HKU2, 2 GDS04_M_ASK51720_1_2017_03_06_China_Swine_Rhinolophus_bat_coronavirus_HKU2, 3 HKU2_GD_430_2006_M_ABQ57211_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2, 4 HKU2_HK_298_2006_M_ABQ57227_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2, 5 HKU2_HK_33_2006_M_ABQ57235_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2, 6 HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2, 7 PEAV_GDS04_P12_M_QBP43303_1_2017_02_China_Swine_Rhinolophus_bat_coronavirus_HKU2, 8 Swine_enteric_alphacoronavirus_CH_GD_01_2017_P2_M_ATN23892_1_2017_China_Swine_Rhinolophus_bat_coronavirus_HKU2 ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:7.512050e-04,7:5.116930e-03,8:1.808583e-03,(2:7.628855e-04,(3:1.108162e-02,(4:7.555266e-04,5:7.197729e-04,6:7.594587e-04)0.962:3.737757e-03)1.000:1.438539e-02)0.999:2.870832e-03); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:7.512050e-04,7:5.116930e-03,8:1.808583e-03,(2:7.628855e-04,(3:1.108162e-02,(4:7.555266e-04,5:7.197729e-04,6:7.594587e-04):3.737757e-03):1.438539e-02):2.870832e-03); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -1138.99 -1150.11 2 -1138.92 -1151.65 -------------------------------------- TOTAL -1138.96 -1151.15 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.049991 0.000094 0.032747 0.070314 0.049216 1169.66 1237.95 1.000 r(A<->C){all} 0.057955 0.001639 0.000054 0.135203 0.048454 367.55 595.99 1.000 r(A<->G){all} 0.308498 0.006472 0.159727 0.472576 0.304598 481.72 484.15 1.000 r(A<->T){all} 0.059258 0.001149 0.007039 0.127443 0.052757 604.63 824.56 1.000 r(C<->G){all} 0.068379 0.002223 0.000070 0.162695 0.059199 366.84 470.88 1.000 r(C<->T){all} 0.430546 0.007266 0.273412 0.598583 0.427641 459.20 488.09 1.000 r(G<->T){all} 0.075364 0.001676 0.008881 0.156847 0.068968 503.68 594.86 1.000 pi(A){all} 0.249189 0.000264 0.218814 0.280954 0.249079 1105.15 1231.64 1.000 pi(C){all} 0.206031 0.000212 0.179091 0.235370 0.205697 1038.33 1079.15 1.000 pi(G){all} 0.210622 0.000231 0.181055 0.239878 0.210508 1238.34 1273.56 1.000 pi(T){all} 0.334158 0.000311 0.300140 0.368910 0.333791 1349.19 1383.28 1.000 alpha{1,2} 0.957541 0.986826 0.000033 2.874150 0.652734 1266.28 1274.06 1.000 alpha{3} 1.334892 1.154283 0.000990 3.348768 1.078726 1082.63 1153.10 1.000 pinvar{all} 0.299916 0.040714 0.000055 0.667298 0.271195 751.07 871.97 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C7,C8,(C2,(C3,(C4,C5,C6))))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **8** sequences, **229** codons, and **1** partitions from `/data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/results/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/results/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result/original_alignment/fubar/results/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1/HKU2_HK_46_2006_M_ABQ57219_1_NA_NA_Unknown_Rhinolophus_bat_coronavirus_HKU2.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -1129.77, AIC-c = 2297.68 (19 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.046 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 0.643 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 2 | 1 | 3.550 | 27.257 | Pos. posterior = 0.9221 | | 4 | 1 | 3.051 | 31.224 | Pos. posterior = 0.9460 | | 7 | 1 | 3.564 | 27.662 | Pos. posterior = 0.9232 | ---- ## FUBAR inferred 3 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.21 are expected to be false positives (95% confidence interval of 0-1 )
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500