--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -839.16 -851.97 2 -838.68 -851.79 -------------------------------------- TOTAL -838.89 -851.88 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.304420 0.004249 0.192208 0.436590 0.295201 867.53 937.34 1.000 r(A<->C){all} 0.087069 0.001616 0.013860 0.160857 0.082410 553.39 620.23 1.000 r(A<->G){all} 0.157403 0.002704 0.069745 0.263839 0.151584 497.65 528.29 1.001 r(A<->T){all} 0.100707 0.001639 0.026705 0.177595 0.096963 349.86 400.34 1.002 r(C<->G){all} 0.053979 0.001010 0.003957 0.117868 0.048972 525.66 679.42 1.001 r(C<->T){all} 0.542941 0.006004 0.404741 0.705339 0.542053 440.86 467.87 1.001 r(G<->T){all} 0.057900 0.001009 0.005587 0.120305 0.052467 398.34 539.83 1.000 pi(A){all} 0.267532 0.000490 0.223611 0.308719 0.267272 1089.42 1190.71 1.001 pi(C){all} 0.260152 0.000492 0.217138 0.303646 0.259480 1168.94 1334.97 1.001 pi(G){all} 0.227061 0.000475 0.186221 0.271200 0.226350 1137.35 1178.08 1.000 pi(T){all} 0.245256 0.000473 0.201398 0.286221 0.245012 1236.80 1282.96 1.000 alpha{1,2} 0.120502 0.011230 0.000061 0.294930 0.100671 1067.70 1113.04 1.000 alpha{3} 2.309624 1.642257 0.473627 4.929037 2.024246 1259.95 1319.39 1.000 pinvar{all} 0.472338 0.013584 0.237283 0.681523 0.479801 1168.29 1183.77 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Tue Oct 25 21:14:53 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=1000, Nseq=9, Len=119 C1 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNIQYPIKWRCIYT C2 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT C3 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT C4 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT C5 MDYVSLLNQVWQKQVNSAHEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT C6 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C7 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C8 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C9 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGTLQYPIKWRCIYT *****************::******:***:*******.:* ********* C1 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C2 FAGYTGTSTEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C3 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C4 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C5 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C6 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C7 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C8 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C9 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN *******:*****.***************: ****** *********::* C1 RYDASKSYFYTQTSSSSNE C2 RYDASKSYFYTETSSSSNE C3 RYDASKSYFYTQTSSSSHE C4 RYDASKSYFYTQTSSSSHE C5 RYDASKSYFYTKTPSSPEE C6 RYDASKSYFYTETSSSSDE C7 RYDASKSYFYTETSSSSDE C8 RYDASKSYFYTETSSSSDE C9 RYDASKSYFYTETSSSSDE ***********:*.**..* -- Starting log on Tue Oct 25 21:15:34 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=9, Len=119 C1 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNIQYPIKWRCIYT C2 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT C3 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT C4 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT C5 MDYVSLLNQVWQKQVNSAHEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT C6 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C7 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C8 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C9 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGTLQYPIKWRCIYT *****************::******:***:*******.:* ********* C1 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C2 FAGYTGTSTEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C3 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C4 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C5 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C6 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C7 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C8 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C9 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN *******:*****.***************: ****** *********::* C1 RYDASKSYFYTQTSSSSNE C2 RYDASKSYFYTETSSSSNE C3 RYDASKSYFYTQTSSSSHE C4 RYDASKSYFYTQTSSSSHE C5 RYDASKSYFYTKTPSSPEE C6 RYDASKSYFYTETSSSSDE C7 RYDASKSYFYTETSSSSDE C8 RYDASKSYFYTETSSSSDE C9 RYDASKSYFYTETSSSSDE ***********:*.**..* -- Starting log on Tue Oct 25 21:14:53 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=1000, Nseq=9, Len=119 C1 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNIQYPIKWRCIYT C2 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT C3 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT C4 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT C5 MDYVSLLNQVWQKQVNSAHEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT C6 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C7 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C8 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT C9 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGTLQYPIKWRCIYT *****************::******:***:*******.:* ********* C1 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C2 FAGYTGTSTEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C3 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C4 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C5 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN C6 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C7 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C8 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN C9 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN *******:*****.***************: ****** *********::* C1 RYDASKSYFYTQTSSSSNE C2 RYDASKSYFYTETSSSSNE C3 RYDASKSYFYTQTSSSSHE C4 RYDASKSYFYTQTSSSSHE C5 RYDASKSYFYTKTPSSPEE C6 RYDASKSYFYTETSSSSDE C7 RYDASKSYFYTETSSSSDE C8 RYDASKSYFYTETSSSSDE C9 RYDASKSYFYTETSSSSDE ***********:*.**..* -- Starting log on Tue Oct 25 21:27:59 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/gapped_alignment/fubar,TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 9 taxa and 357 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C2 Taxon 3 -> C3 Taxon 4 -> C4 Taxon 5 -> C5 Taxon 6 -> C6 Taxon 7 -> C7 Taxon 8 -> C8 Taxon 9 -> C9 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1666733281 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 288980266 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 4282605921 Seed = 1802050743 Swapseed = 1666733281 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 14 unique site patterns Division 2 has 10 unique site patterns Division 3 has 33 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -1030.159173 -- 32.479477 Chain 2 -- -998.759157 -- 32.479477 Chain 3 -- -1057.347696 -- 32.479477 Chain 4 -- -1058.048168 -- 32.479477 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -1033.371573 -- 32.479477 Chain 2 -- -1021.477098 -- 32.479477 Chain 3 -- -1001.796756 -- 32.479477 Chain 4 -- -1020.197557 -- 32.479477 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-1030.159] (-998.759) (-1057.348) (-1058.048) * [-1033.372] (-1021.477) (-1001.797) (-1020.198) 1000 -- (-860.468) [-859.288] (-872.267) (-868.349) * (-868.587) (-870.384) [-860.544] (-869.107) -- 0:00:00 2000 -- (-862.242) (-857.048) (-865.316) [-847.027] * (-846.312) [-854.615] (-849.141) (-868.632) -- 0:08:19 3000 -- (-853.596) (-853.796) (-867.747) [-844.929] * [-843.300] (-853.321) (-844.731) (-866.270) -- 0:05:32 4000 -- (-844.815) (-852.832) (-871.355) [-847.394] * (-850.776) (-849.916) [-848.427] (-856.378) -- 0:04:09 5000 -- [-845.597] (-850.055) (-866.674) (-852.251) * (-845.891) (-849.969) [-840.232] (-851.412) -- 0:03:19 Average standard deviation of split frequencies: 0.042855 6000 -- [-847.293] (-844.441) (-865.239) (-843.287) * (-851.650) (-851.432) (-844.504) [-844.199] -- 0:05:31 7000 -- (-853.176) [-840.711] (-857.432) (-858.026) * [-841.426] (-855.361) (-848.332) (-843.838) -- 0:04:43 8000 -- (-844.542) [-845.885] (-855.801) (-856.603) * [-841.732] (-851.699) (-845.449) (-840.434) -- 0:04:08 9000 -- (-847.763) (-844.388) (-852.441) [-842.169] * [-842.818] (-848.468) (-852.002) (-849.322) -- 0:03:40 10000 -- (-849.914) (-843.083) [-851.654] (-846.490) * [-844.343] (-848.359) (-849.346) (-845.145) -- 0:04:57 Average standard deviation of split frequencies: 0.056247 11000 -- (-838.953) [-845.930] (-849.856) (-841.085) * (-862.659) (-852.488) (-851.485) [-842.093] -- 0:04:29 12000 -- (-841.116) [-846.186] (-848.232) (-844.106) * (-855.205) [-845.057] (-851.245) (-843.413) -- 0:04:07 13000 -- (-845.594) (-840.115) [-844.684] (-847.448) * (-839.814) (-843.330) (-846.486) [-839.830] -- 0:03:47 14000 -- (-842.388) [-838.083] (-848.577) (-844.040) * (-841.895) [-841.140] (-847.752) (-843.425) -- 0:04:41 15000 -- (-841.964) (-848.515) [-847.767] (-850.973) * (-853.098) (-844.645) [-841.332] (-855.224) -- 0:04:22 Average standard deviation of split frequencies: 0.050890 16000 -- (-848.104) [-852.070] (-844.821) (-846.556) * (-849.180) (-848.912) [-841.585] (-839.226) -- 0:04:06 17000 -- (-847.822) (-844.008) [-847.186] (-845.539) * [-843.881] (-844.134) (-847.507) (-843.068) -- 0:03:51 18000 -- [-834.670] (-842.126) (-846.760) (-855.076) * (-845.483) [-843.288] (-846.537) (-845.792) -- 0:04:32 19000 -- (-846.372) (-841.815) (-853.574) [-838.978] * (-844.591) (-854.059) (-843.272) [-844.102] -- 0:04:18 20000 -- (-840.796) (-842.582) [-839.651] (-843.698) * [-844.577] (-853.163) (-852.071) (-843.129) -- 0:04:05 Average standard deviation of split frequencies: 0.058062 21000 -- (-840.409) (-850.942) [-838.823] (-852.565) * (-839.690) (-846.989) [-843.923] (-846.369) -- 0:03:53 22000 -- (-841.130) [-842.368] (-840.637) (-850.293) * (-857.243) (-841.372) [-837.033] (-848.338) -- 0:04:26 23000 -- [-844.229] (-838.979) (-838.929) (-842.170) * (-848.360) (-845.142) [-849.384] (-848.215) -- 0:04:14 24000 -- [-849.075] (-851.631) (-846.673) (-841.851) * (-844.981) (-844.893) (-844.027) [-841.091] -- 0:04:04 25000 -- (-848.372) (-844.266) (-840.905) [-845.305] * (-845.540) (-853.222) [-843.623] (-845.604) -- 0:03:54 Average standard deviation of split frequencies: 0.046151 26000 -- (-852.080) (-848.186) (-837.926) [-844.587] * (-848.816) (-844.551) (-843.081) [-846.845] -- 0:04:22 27000 -- (-843.185) (-841.625) (-840.433) [-840.100] * [-841.537] (-847.030) (-842.708) (-844.125) -- 0:04:12 28000 -- (-848.698) [-844.017] (-845.275) (-841.899) * (-844.043) [-852.236] (-846.532) (-845.734) -- 0:04:03 29000 -- [-849.566] (-843.379) (-843.438) (-844.756) * (-836.995) [-847.340] (-860.730) (-843.558) -- 0:04:27 30000 -- (-855.562) [-846.983] (-846.695) (-846.545) * [-843.776] (-844.721) (-840.444) (-844.214) -- 0:04:18 Average standard deviation of split frequencies: 0.033539 31000 -- [-846.347] (-842.139) (-842.908) (-845.093) * (-843.098) [-836.504] (-842.595) (-849.217) -- 0:04:10 32000 -- (-850.811) (-837.196) [-839.505] (-845.947) * (-839.001) [-844.792] (-840.754) (-851.675) -- 0:04:02 33000 -- (-843.290) [-843.889] (-848.386) (-853.198) * (-843.015) (-847.074) (-848.241) [-849.862] -- 0:04:23 34000 -- (-843.662) (-845.259) [-843.379] (-841.910) * (-846.085) [-838.034] (-842.135) (-844.574) -- 0:04:15 35000 -- (-847.027) (-845.074) [-844.554] (-851.113) * (-845.116) [-845.586] (-845.821) (-840.961) -- 0:04:08 Average standard deviation of split frequencies: 0.051188 36000 -- (-846.661) (-846.928) [-838.079] (-846.042) * (-850.765) [-843.077] (-841.269) (-845.106) -- 0:04:01 37000 -- [-849.415] (-851.742) (-837.857) (-841.520) * (-841.236) (-845.092) (-847.996) [-843.872] -- 0:04:20 38000 -- (-848.440) (-847.792) [-848.664] (-848.293) * (-844.062) (-850.603) [-844.374] (-843.924) -- 0:04:13 39000 -- [-852.059] (-847.093) (-842.526) (-849.437) * (-843.583) [-848.980] (-850.037) (-855.969) -- 0:04:06 40000 -- (-844.079) (-855.330) [-844.793] (-851.294) * [-846.564] (-850.021) (-846.726) (-843.945) -- 0:04:00 Average standard deviation of split frequencies: 0.041099 41000 -- (-841.159) [-839.458] (-845.512) (-848.650) * [-843.404] (-845.873) (-850.379) (-846.298) -- 0:04:17 42000 -- (-840.217) [-839.959] (-839.635) (-846.093) * (-841.128) (-855.520) [-841.848] (-844.639) -- 0:04:10 43000 -- (-843.211) (-839.103) [-836.563] (-845.329) * (-838.126) (-841.165) (-843.413) [-843.834] -- 0:04:04 44000 -- (-843.146) [-842.879] (-844.638) (-844.743) * [-849.610] (-856.757) (-849.311) (-847.793) -- 0:03:59 45000 -- (-851.960) (-850.776) (-846.789) [-843.295] * (-842.724) [-839.498] (-847.905) (-852.406) -- 0:04:14 Average standard deviation of split frequencies: 0.027949 46000 -- (-845.186) (-848.641) (-846.268) [-848.013] * (-840.864) (-841.675) [-841.509] (-846.832) -- 0:04:08 47000 -- (-853.279) (-841.045) (-846.442) [-844.183] * (-845.438) (-843.605) [-842.868] (-840.386) -- 0:04:03 48000 -- (-840.543) (-847.923) (-853.887) [-842.616] * (-846.848) (-846.596) [-840.318] (-839.288) -- 0:03:58 49000 -- (-846.951) [-837.570] (-849.353) (-843.451) * (-848.294) (-846.898) [-845.465] (-848.362) -- 0:04:12 50000 -- (-854.190) (-843.914) [-844.494] (-839.522) * (-849.995) (-852.164) [-843.158] (-850.331) -- 0:04:06 Average standard deviation of split frequencies: 0.030450 51000 -- (-851.385) (-846.998) (-846.157) [-839.122] * [-839.376] (-848.475) (-847.150) (-847.437) -- 0:04:01 52000 -- (-854.551) [-841.396] (-855.542) (-849.442) * (-842.792) (-843.750) (-840.426) [-844.646] -- 0:03:57 53000 -- [-846.460] (-848.344) (-844.854) (-851.362) * [-840.039] (-845.693) (-844.890) (-847.458) -- 0:04:10 54000 -- (-850.588) (-847.723) (-852.064) [-847.340] * (-841.036) (-846.153) [-840.449] (-842.391) -- 0:04:05 55000 -- [-851.216] (-840.847) (-844.927) (-852.760) * (-848.500) (-850.007) [-844.523] (-854.557) -- 0:04:00 Average standard deviation of split frequencies: 0.029845 56000 -- (-847.316) (-847.444) (-851.354) [-838.160] * (-844.666) (-848.405) (-839.450) [-847.302] -- 0:03:56 57000 -- (-839.477) [-842.109] (-842.502) (-843.492) * (-844.918) [-840.655] (-852.470) (-840.014) -- 0:04:08 58000 -- (-845.770) [-838.010] (-847.072) (-842.738) * (-853.113) (-846.055) (-849.322) [-850.717] -- 0:04:03 59000 -- (-848.540) [-847.581] (-849.827) (-842.242) * (-845.445) [-845.881] (-844.968) (-852.065) -- 0:03:59 60000 -- (-851.439) [-843.099] (-853.736) (-854.649) * (-850.816) (-863.553) (-844.375) [-842.382] -- 0:03:55 Average standard deviation of split frequencies: 0.024018 61000 -- (-842.222) (-845.662) (-843.574) [-841.763] * (-846.720) (-854.319) (-851.819) [-835.006] -- 0:04:06 62000 -- (-848.830) (-845.909) (-846.361) [-840.846] * (-841.537) (-847.462) (-845.809) [-845.336] -- 0:04:02 63000 -- (-843.450) (-851.713) (-861.691) [-840.445] * (-844.771) (-854.628) (-847.957) [-840.290] -- 0:03:57 64000 -- [-843.475] (-851.818) (-848.596) (-836.337) * (-842.892) [-839.551] (-847.504) (-847.267) -- 0:03:54 65000 -- (-847.120) (-849.844) [-844.702] (-841.385) * (-848.128) (-851.763) (-850.274) [-846.192] -- 0:04:04 Average standard deviation of split frequencies: 0.028570 66000 -- [-840.103] (-845.515) (-841.509) (-840.802) * (-857.077) [-848.186] (-843.777) (-837.187) -- 0:04:00 67000 -- [-850.189] (-849.602) (-841.285) (-846.692) * (-854.567) (-842.764) (-842.780) [-837.518] -- 0:03:56 68000 -- (-847.264) [-845.650] (-851.449) (-842.043) * (-844.796) (-850.743) (-851.572) [-837.709] -- 0:03:53 69000 -- (-847.697) [-849.554] (-842.309) (-850.762) * (-849.946) (-843.274) [-839.247] (-840.693) -- 0:04:02 70000 -- (-859.394) (-844.221) (-848.540) [-841.789] * (-852.351) (-846.152) (-842.879) [-840.025] -- 0:03:59 Average standard deviation of split frequencies: 0.032748 71000 -- (-854.119) (-847.547) [-848.578] (-848.334) * (-844.953) [-850.124] (-850.376) (-844.653) -- 0:03:55 72000 -- [-844.627] (-843.418) (-847.400) (-848.208) * (-853.983) (-843.072) (-845.780) [-842.668] -- 0:03:52 73000 -- [-845.869] (-846.218) (-845.209) (-853.465) * (-849.973) (-837.314) [-838.288] (-844.072) -- 0:04:01 74000 -- (-844.782) (-843.750) [-845.548] (-847.100) * (-852.460) (-848.681) (-846.899) [-842.261] -- 0:03:57 75000 -- (-844.353) [-839.836] (-848.974) (-850.495) * (-848.428) [-839.414] (-847.708) (-845.177) -- 0:03:54 Average standard deviation of split frequencies: 0.036088 76000 -- (-844.792) (-845.306) [-845.659] (-842.628) * (-846.924) (-841.973) (-840.282) [-842.700] -- 0:03:51 77000 -- (-844.845) (-846.966) [-842.829] (-844.728) * (-847.795) (-848.573) [-838.865] (-843.617) -- 0:03:59 78000 -- (-842.076) (-845.497) (-848.825) [-848.409] * (-848.925) (-844.009) [-841.858] (-840.004) -- 0:03:56 79000 -- [-835.802] (-843.328) (-846.788) (-847.639) * [-844.531] (-841.748) (-846.094) (-842.696) -- 0:03:53 80000 -- [-842.487] (-848.254) (-849.623) (-843.134) * (-846.013) [-841.776] (-843.120) (-845.487) -- 0:03:50 Average standard deviation of split frequencies: 0.031876 81000 -- (-845.022) (-847.666) (-858.445) [-843.832] * (-845.929) (-845.501) [-841.872] (-839.228) -- 0:03:58 82000 -- (-857.804) [-841.664] (-844.775) (-841.462) * (-843.294) (-848.718) (-842.815) [-848.062] -- 0:03:55 83000 -- (-845.472) [-842.519] (-847.484) (-852.060) * (-848.424) [-849.062] (-844.437) (-853.281) -- 0:03:52 84000 -- [-839.130] (-846.167) (-851.260) (-846.193) * (-844.353) (-862.042) [-851.244] (-838.929) -- 0:03:49 85000 -- (-837.548) (-851.701) (-850.028) [-846.047] * (-842.707) (-849.466) (-844.588) [-839.750] -- 0:03:56 Average standard deviation of split frequencies: 0.027906 86000 -- (-844.319) [-842.065] (-847.497) (-844.183) * (-845.375) [-850.293] (-849.574) (-843.391) -- 0:03:53 87000 -- [-843.192] (-850.872) (-851.652) (-845.685) * (-854.791) (-846.720) [-845.648] (-843.726) -- 0:03:50 88000 -- (-849.820) (-841.923) [-843.255] (-842.962) * (-842.424) (-847.864) [-849.779] (-846.029) -- 0:03:48 89000 -- (-839.957) [-842.419] (-840.808) (-845.261) * (-842.563) [-853.036] (-853.931) (-843.705) -- 0:03:55 90000 -- (-847.009) (-846.376) [-839.506] (-847.780) * [-847.825] (-840.524) (-851.119) (-852.974) -- 0:03:52 Average standard deviation of split frequencies: 0.024579 91000 -- (-844.961) (-846.801) (-840.636) [-844.260] * (-845.121) [-850.817] (-845.349) (-844.977) -- 0:03:49 92000 -- (-847.379) (-844.459) [-843.678] (-847.432) * [-836.397] (-843.567) (-851.731) (-848.935) -- 0:03:47 93000 -- (-845.826) (-840.697) [-846.720] (-849.875) * [-841.565] (-845.250) (-855.360) (-853.037) -- 0:03:54 94000 -- [-841.875] (-846.360) (-850.874) (-844.747) * (-844.295) (-846.595) (-849.361) [-842.691] -- 0:03:51 95000 -- [-841.290] (-842.031) (-835.791) (-846.220) * (-840.761) (-854.482) [-844.553] (-837.187) -- 0:03:48 Average standard deviation of split frequencies: 0.021874 96000 -- (-850.947) (-842.483) [-846.608] (-846.363) * [-846.897] (-849.657) (-844.273) (-846.493) -- 0:03:46 97000 -- [-841.745] (-843.472) (-846.437) (-843.528) * (-837.947) (-844.418) (-846.106) [-839.773] -- 0:03:52 98000 -- (-846.228) (-847.871) (-850.564) [-837.827] * [-836.645] (-849.843) (-845.200) (-843.771) -- 0:03:50 99000 -- (-844.466) (-847.997) [-849.737] (-857.978) * (-842.962) (-859.654) (-841.961) [-844.946] -- 0:03:47 100000 -- [-842.939] (-842.673) (-842.430) (-843.838) * (-842.061) (-850.260) (-840.497) [-836.958] -- 0:03:45 Average standard deviation of split frequencies: 0.023840 101000 -- (-848.447) (-850.827) (-844.346) [-843.216] * [-843.748] (-843.163) (-847.801) (-840.623) -- 0:03:51 102000 -- (-847.107) (-849.239) (-844.969) [-841.631] * [-837.289] (-856.748) (-849.834) (-842.675) -- 0:03:48 103000 -- (-844.400) (-846.838) [-837.991] (-841.903) * [-847.956] (-853.047) (-849.690) (-847.725) -- 0:03:46 104000 -- [-842.876] (-849.145) (-844.488) (-847.197) * (-854.469) (-851.024) [-839.980] (-846.163) -- 0:03:44 105000 -- [-843.023] (-848.733) (-848.956) (-842.504) * (-839.171) [-851.183] (-844.799) (-845.184) -- 0:03:50 Average standard deviation of split frequencies: 0.020215 106000 -- (-841.830) (-839.629) [-840.746] (-846.573) * (-839.119) [-848.948] (-841.156) (-849.372) -- 0:03:47 107000 -- (-850.508) [-840.400] (-843.677) (-843.137) * (-843.811) (-855.553) [-837.629] (-840.073) -- 0:03:45 108000 -- (-846.926) (-844.272) [-842.890] (-847.978) * (-855.580) [-841.240] (-843.624) (-842.548) -- 0:03:51 109000 -- (-843.780) (-843.484) [-836.429] (-845.703) * (-844.854) (-864.201) (-846.165) [-842.105] -- 0:03:48 110000 -- [-844.371] (-851.316) (-844.517) (-838.799) * (-842.640) [-845.759] (-845.211) (-844.358) -- 0:03:46 Average standard deviation of split frequencies: 0.020137 111000 -- [-843.323] (-855.410) (-840.980) (-847.816) * (-844.166) [-846.586] (-845.466) (-844.766) -- 0:03:44 112000 -- (-843.700) (-843.015) [-844.828] (-841.918) * (-844.018) (-849.948) (-842.557) [-841.435] -- 0:03:42 113000 -- (-842.264) (-848.808) (-837.391) [-851.825] * (-845.338) (-847.791) [-838.489] (-851.645) -- 0:03:47 114000 -- (-840.802) (-844.073) [-839.568] (-855.803) * (-852.513) (-847.483) [-836.939] (-846.838) -- 0:03:45 115000 -- [-839.714] (-840.791) (-846.469) (-842.852) * (-843.485) (-843.887) (-843.133) [-846.526] -- 0:03:43 Average standard deviation of split frequencies: 0.017364 116000 -- [-842.753] (-846.967) (-839.279) (-850.039) * [-845.492] (-846.061) (-849.318) (-842.192) -- 0:03:41 117000 -- [-838.113] (-836.919) (-842.718) (-848.109) * [-850.106] (-842.845) (-843.345) (-852.781) -- 0:03:46 118000 -- (-840.466) (-848.120) (-838.724) [-836.641] * (-851.008) (-839.813) [-844.376] (-846.057) -- 0:03:44 119000 -- (-847.255) (-847.075) (-842.468) [-839.274] * (-847.193) [-840.365] (-840.379) (-844.682) -- 0:03:42 120000 -- (-834.586) (-847.847) [-842.367] (-846.471) * (-854.773) [-842.291] (-838.970) (-847.620) -- 0:03:40 Average standard deviation of split frequencies: 0.014561 121000 -- (-847.108) (-849.714) (-849.899) [-849.227] * [-840.705] (-843.785) (-842.793) (-852.551) -- 0:03:45 122000 -- (-836.512) (-845.620) (-844.978) [-849.013] * (-845.142) (-838.276) (-846.027) [-840.066] -- 0:03:43 123000 -- (-839.802) [-844.463] (-847.441) (-848.786) * [-842.299] (-849.055) (-853.025) (-846.234) -- 0:03:41 124000 -- (-843.688) (-846.167) (-852.934) [-848.014] * [-842.935] (-850.146) (-845.028) (-838.388) -- 0:03:46 125000 -- (-847.710) (-855.524) (-852.936) [-840.938] * (-844.476) (-849.387) (-843.071) [-836.835] -- 0:03:44 Average standard deviation of split frequencies: 0.020747 126000 -- [-844.864] (-851.042) (-854.124) (-850.482) * (-845.250) (-844.380) [-841.278] (-847.386) -- 0:03:41 127000 -- [-844.711] (-850.738) (-844.280) (-851.761) * (-845.856) (-844.439) (-852.015) [-846.154] -- 0:03:39 128000 -- (-846.132) (-843.216) [-843.794] (-855.146) * (-844.638) [-843.288] (-846.717) (-855.172) -- 0:03:44 129000 -- (-858.122) (-848.838) (-850.529) [-850.605] * [-840.166] (-851.481) (-845.104) (-842.898) -- 0:03:42 130000 -- (-844.172) (-839.494) [-841.997] (-839.404) * (-849.806) [-842.656] (-844.411) (-854.933) -- 0:03:40 Average standard deviation of split frequencies: 0.019350 131000 -- (-849.571) [-845.444] (-843.372) (-848.199) * (-846.409) [-844.306] (-848.455) (-850.797) -- 0:03:38 132000 -- (-857.543) [-843.365] (-844.144) (-843.748) * (-847.038) (-847.834) [-838.562] (-847.320) -- 0:03:43 133000 -- (-863.979) (-845.855) (-842.385) [-841.902] * (-849.921) [-843.198] (-856.180) (-843.489) -- 0:03:41 134000 -- (-853.149) (-858.653) [-845.109] (-842.661) * (-848.861) [-845.633] (-849.969) (-843.086) -- 0:03:39 135000 -- (-849.085) (-849.896) [-845.183] (-842.573) * (-847.217) (-846.998) [-843.516] (-849.388) -- 0:03:37 Average standard deviation of split frequencies: 0.013550 136000 -- (-846.783) (-851.113) [-837.002] (-847.835) * (-856.269) (-842.521) (-847.227) [-843.072] -- 0:03:42 137000 -- (-846.578) (-843.338) (-843.821) [-840.831] * (-848.452) (-847.489) (-840.844) [-846.210] -- 0:03:40 138000 -- (-854.482) [-847.070] (-842.392) (-848.966) * (-853.314) (-846.960) (-841.653) [-843.095] -- 0:03:38 139000 -- [-848.664] (-849.977) (-841.886) (-846.331) * (-843.418) (-846.659) (-852.689) [-843.278] -- 0:03:42 140000 -- (-847.091) (-841.170) (-846.324) [-847.489] * [-844.950] (-839.341) (-846.465) (-843.159) -- 0:03:41 Average standard deviation of split frequencies: 0.011577 141000 -- [-844.486] (-843.532) (-847.300) (-850.145) * (-849.369) [-846.419] (-845.065) (-842.855) -- 0:03:39 142000 -- [-844.380] (-849.333) (-845.912) (-853.908) * (-842.780) [-846.470] (-847.861) (-848.489) -- 0:03:37 143000 -- (-843.549) (-849.757) [-851.215] (-846.931) * (-839.887) (-840.233) (-843.509) [-842.970] -- 0:03:41 144000 -- (-839.134) (-841.507) (-847.314) [-844.897] * (-845.163) [-836.389] (-848.846) (-842.827) -- 0:03:39 145000 -- (-847.573) (-842.791) (-852.158) [-846.084] * [-843.504] (-850.618) (-843.101) (-846.985) -- 0:03:38 Average standard deviation of split frequencies: 0.007632 146000 -- (-855.983) (-838.550) [-842.583] (-842.795) * [-840.643] (-841.091) (-852.526) (-850.731) -- 0:03:36 147000 -- [-838.406] (-849.875) (-844.183) (-845.800) * (-853.203) (-843.752) (-841.831) [-837.394] -- 0:03:40 148000 -- (-847.629) [-840.659] (-851.796) (-842.874) * (-846.615) (-841.110) (-851.038) [-840.265] -- 0:03:38 149000 -- (-850.073) (-846.170) (-841.914) [-849.229] * (-848.138) [-849.918] (-847.058) (-848.859) -- 0:03:37 150000 -- (-845.103) (-849.186) (-842.189) [-841.286] * (-848.262) [-838.923] (-842.642) (-850.699) -- 0:03:35 Average standard deviation of split frequencies: 0.008533 151000 -- (-848.937) (-842.984) [-841.852] (-847.678) * (-845.313) (-838.258) [-839.121] (-849.533) -- 0:03:39 152000 -- (-843.562) (-847.655) (-843.248) [-842.496] * (-843.005) (-846.601) [-838.608] (-842.277) -- 0:03:37 153000 -- (-848.033) (-847.763) [-844.611] (-853.382) * (-847.220) (-853.006) (-843.602) [-844.912] -- 0:03:35 154000 -- (-842.721) (-840.374) (-851.114) [-842.179] * [-847.148] (-846.822) (-841.477) (-844.781) -- 0:03:34 155000 -- (-843.110) [-835.665] (-851.105) (-844.801) * [-844.597] (-842.245) (-843.448) (-845.725) -- 0:03:38 Average standard deviation of split frequencies: 0.007417 156000 -- (-842.268) (-845.044) (-848.713) [-849.322] * (-845.802) [-842.558] (-845.205) (-851.307) -- 0:03:36 157000 -- (-843.339) [-842.069] (-846.789) (-843.758) * [-849.907] (-852.147) (-840.493) (-843.997) -- 0:03:34 158000 -- (-841.196) [-840.404] (-845.884) (-845.885) * (-846.598) (-848.065) (-845.063) [-846.260] -- 0:03:33 159000 -- (-839.521) (-844.852) (-852.861) [-843.293] * [-850.332] (-846.806) (-843.243) (-862.088) -- 0:03:36 160000 -- (-846.777) (-853.860) (-843.801) [-839.800] * [-837.370] (-837.051) (-843.209) (-843.804) -- 0:03:35 Average standard deviation of split frequencies: 0.008002 161000 -- (-839.508) (-843.937) (-852.452) [-840.572] * (-845.388) [-843.377] (-849.442) (-846.448) -- 0:03:33 162000 -- (-841.271) (-851.836) (-846.805) [-848.515] * (-846.781) (-844.814) (-850.439) [-843.255] -- 0:03:32 163000 -- (-844.106) (-847.509) [-841.874] (-844.223) * (-838.674) [-846.434] (-844.428) (-847.410) -- 0:03:35 164000 -- (-838.789) [-840.968] (-847.161) (-841.345) * (-847.030) [-838.549] (-851.474) (-854.361) -- 0:03:34 165000 -- (-846.341) (-856.530) (-844.557) [-842.184] * (-854.581) [-846.306] (-843.929) (-847.679) -- 0:03:32 Average standard deviation of split frequencies: 0.008261 166000 -- [-851.255] (-840.153) (-842.217) (-846.083) * (-845.833) (-845.676) (-847.841) [-841.526] -- 0:03:31 167000 -- (-845.921) [-840.019] (-842.031) (-843.410) * (-854.027) [-843.343] (-846.785) (-845.056) -- 0:03:34 168000 -- [-841.384] (-839.863) (-848.884) (-848.248) * (-854.174) [-845.980] (-842.912) (-840.164) -- 0:03:32 169000 -- (-840.554) [-839.156] (-848.418) (-848.908) * (-850.600) (-847.187) [-846.672] (-840.802) -- 0:03:31 170000 -- [-844.086] (-864.018) (-847.590) (-854.669) * (-843.508) (-843.139) [-839.153] (-840.493) -- 0:03:29 Average standard deviation of split frequencies: 0.007282 171000 -- (-842.324) (-849.284) (-861.299) [-856.953] * (-847.396) (-846.191) (-849.813) [-845.895] -- 0:03:33 172000 -- (-847.219) (-854.794) (-848.177) [-854.402] * (-845.903) [-845.989] (-853.295) (-842.071) -- 0:03:31 173000 -- (-847.732) [-844.018] (-843.903) (-849.200) * (-848.860) (-839.719) [-845.959] (-845.309) -- 0:03:30 174000 -- (-838.899) [-844.020] (-851.454) (-851.872) * (-857.016) (-844.969) [-841.247] (-851.927) -- 0:03:28 175000 -- (-842.890) (-844.865) [-842.653] (-846.067) * (-855.487) (-840.913) (-846.479) [-841.203] -- 0:03:32 Average standard deviation of split frequencies: 0.011931 176000 -- [-849.634] (-840.477) (-852.177) (-846.119) * (-847.543) [-841.230] (-847.918) (-843.183) -- 0:03:30 177000 -- (-853.247) [-845.374] (-846.468) (-839.914) * (-841.896) (-844.729) [-844.203] (-853.064) -- 0:03:29 178000 -- (-853.450) (-837.473) (-853.573) [-843.688] * (-842.784) [-840.216] (-844.154) (-851.591) -- 0:03:27 179000 -- [-838.390] (-848.168) (-851.340) (-859.368) * [-839.816] (-847.552) (-842.494) (-846.236) -- 0:03:30 180000 -- (-846.052) [-847.512] (-849.526) (-849.845) * [-841.230] (-847.351) (-840.263) (-845.164) -- 0:03:29 Average standard deviation of split frequencies: 0.015181 181000 -- (-837.716) (-848.829) (-842.869) [-841.622] * (-845.727) [-844.004] (-842.362) (-845.192) -- 0:03:28 182000 -- (-851.169) (-853.457) [-844.010] (-843.634) * (-850.305) (-846.626) [-854.700] (-848.270) -- 0:03:26 183000 -- [-838.491] (-840.348) (-845.438) (-840.171) * (-847.355) (-848.760) (-850.865) [-842.539] -- 0:03:29 184000 -- [-838.168] (-845.090) (-845.864) (-845.299) * (-842.414) (-847.184) [-848.846] (-840.642) -- 0:03:28 185000 -- (-845.109) (-852.284) (-844.375) [-837.468] * [-848.062] (-847.013) (-847.053) (-847.476) -- 0:03:27 Average standard deviation of split frequencies: 0.013363 186000 -- (-848.275) [-848.559] (-846.715) (-843.169) * (-843.503) (-832.679) [-845.647] (-844.872) -- 0:03:25 187000 -- [-842.522] (-849.001) (-843.015) (-840.591) * (-843.042) (-842.319) (-844.650) [-844.690] -- 0:03:28 188000 -- (-847.416) [-852.772] (-844.202) (-845.560) * (-847.122) (-840.892) (-841.259) [-840.159] -- 0:03:27 189000 -- (-848.793) (-853.191) (-840.977) [-840.055] * (-839.673) (-846.749) [-851.149] (-838.386) -- 0:03:25 190000 -- (-843.790) (-854.314) (-840.676) [-843.835] * (-843.638) (-843.262) [-841.665] (-843.660) -- 0:03:28 Average standard deviation of split frequencies: 0.013261 191000 -- (-848.101) (-852.489) [-838.832] (-851.453) * (-844.448) (-848.990) [-840.098] (-843.196) -- 0:03:27 192000 -- [-838.656] (-855.187) (-839.238) (-847.903) * (-844.943) (-846.573) (-850.219) [-840.120] -- 0:03:26 193000 -- (-841.334) (-849.701) [-841.013] (-852.988) * (-850.904) (-851.968) (-852.516) [-845.794] -- 0:03:24 194000 -- (-840.285) [-844.845] (-850.147) (-840.109) * (-844.554) (-840.506) [-846.689] (-847.799) -- 0:03:27 195000 -- (-846.883) (-849.732) (-841.603) [-841.968] * (-850.080) [-837.760] (-841.643) (-839.318) -- 0:03:26 Average standard deviation of split frequencies: 0.010932 196000 -- (-838.255) [-843.067] (-845.079) (-845.107) * (-845.297) (-849.624) (-846.716) [-835.858] -- 0:03:25 197000 -- [-841.303] (-846.424) (-840.159) (-845.725) * (-843.376) [-840.287] (-845.224) (-838.630) -- 0:03:23 198000 -- (-841.052) (-842.309) (-841.620) [-841.890] * (-837.905) (-848.379) [-841.719] (-850.507) -- 0:03:26 199000 -- (-846.071) (-843.336) [-841.757] (-842.841) * [-848.952] (-843.952) (-845.279) (-848.277) -- 0:03:25 200000 -- (-854.303) (-844.110) [-841.874] (-841.416) * (-854.005) [-847.493] (-852.330) (-849.506) -- 0:03:24 Average standard deviation of split frequencies: 0.011960 201000 -- (-846.863) [-839.182] (-845.754) (-843.689) * (-843.487) (-834.365) (-841.989) [-842.400] -- 0:03:22 202000 -- (-851.501) (-844.520) (-838.129) [-846.117] * (-852.808) (-840.495) [-847.052] (-843.564) -- 0:03:25 203000 -- [-838.198] (-846.016) (-841.954) (-841.842) * (-846.483) (-847.817) (-849.285) [-836.395] -- 0:03:24 204000 -- (-843.494) (-841.769) (-843.264) [-852.823] * (-843.482) [-841.801] (-842.589) (-841.731) -- 0:03:22 205000 -- [-841.563] (-841.813) (-839.449) (-847.837) * (-845.839) (-845.774) [-851.084] (-841.513) -- 0:03:21 Average standard deviation of split frequencies: 0.012482 206000 -- [-840.560] (-841.646) (-843.127) (-844.497) * (-843.720) (-841.373) (-853.793) [-844.356] -- 0:03:24 207000 -- [-842.567] (-852.263) (-838.860) (-841.233) * (-851.509) [-855.060] (-840.747) (-851.391) -- 0:03:23 208000 -- (-843.941) (-848.473) [-843.077] (-856.777) * (-842.683) (-846.313) [-837.732] (-844.813) -- 0:03:21 209000 -- (-858.496) [-842.987] (-840.453) (-847.684) * (-851.174) [-842.906] (-847.575) (-840.098) -- 0:03:20 210000 -- (-847.522) [-842.459] (-837.179) (-840.369) * (-843.643) (-852.552) [-850.072] (-841.178) -- 0:03:23 Average standard deviation of split frequencies: 0.012206 211000 -- (-855.950) (-847.404) (-844.304) [-838.291] * (-845.105) [-838.772] (-846.775) (-847.305) -- 0:03:21 212000 -- (-842.299) (-846.644) (-839.866) [-854.644] * (-851.936) (-848.826) [-838.599] (-838.714) -- 0:03:20 213000 -- (-844.935) (-844.480) (-844.665) [-842.621] * (-844.238) [-842.969] (-845.634) (-844.746) -- 0:03:19 214000 -- (-849.397) [-840.851] (-843.604) (-843.526) * (-848.596) [-847.718] (-851.986) (-844.843) -- 0:03:22 215000 -- (-841.360) [-839.438] (-850.827) (-844.901) * (-846.302) (-844.513) [-840.784] (-841.772) -- 0:03:20 Average standard deviation of split frequencies: 0.013095 216000 -- (-842.612) (-846.494) [-843.399] (-849.186) * (-851.304) (-842.324) (-852.377) [-840.955] -- 0:03:19 217000 -- (-841.427) (-856.616) (-844.143) [-841.966] * (-848.021) (-842.755) [-838.991] (-853.895) -- 0:03:22 218000 -- (-859.287) (-848.862) [-850.214] (-845.072) * [-843.094] (-851.979) (-847.103) (-843.042) -- 0:03:20 219000 -- [-845.167] (-847.613) (-849.333) (-842.291) * [-846.203] (-846.973) (-850.332) (-842.396) -- 0:03:19 220000 -- [-848.209] (-849.663) (-846.677) (-842.641) * (-849.980) (-857.340) [-841.365] (-841.526) -- 0:03:18 Average standard deviation of split frequencies: 0.013400 221000 -- (-848.637) (-843.693) (-846.055) [-840.206] * [-843.723] (-859.132) (-839.173) (-846.225) -- 0:03:20 222000 -- [-846.530] (-849.171) (-846.791) (-846.921) * [-838.739] (-855.121) (-838.645) (-844.613) -- 0:03:19 223000 -- (-856.526) (-854.894) (-843.410) [-844.212] * [-844.659] (-849.998) (-842.023) (-851.408) -- 0:03:18 224000 -- (-848.290) [-845.057] (-840.662) (-845.700) * [-850.330] (-854.800) (-846.769) (-845.081) -- 0:03:17 225000 -- (-840.485) (-852.248) [-842.390] (-843.116) * (-848.713) [-852.590] (-840.971) (-844.063) -- 0:03:19 Average standard deviation of split frequencies: 0.014411 226000 -- (-847.206) [-846.680] (-845.109) (-845.014) * [-842.849] (-852.577) (-849.093) (-843.403) -- 0:03:18 227000 -- (-844.827) (-846.514) [-842.155] (-845.797) * [-851.707] (-849.885) (-845.128) (-846.674) -- 0:03:17 228000 -- (-853.090) (-840.772) [-849.772] (-839.722) * (-842.009) (-847.912) [-844.458] (-850.106) -- 0:03:16 229000 -- (-855.750) (-843.651) [-840.274] (-845.450) * [-842.738] (-840.639) (-844.004) (-842.531) -- 0:03:18 230000 -- (-849.523) (-845.331) (-846.439) [-840.762] * (-847.404) (-850.181) (-843.160) [-838.918] -- 0:03:17 Average standard deviation of split frequencies: 0.016721 231000 -- (-851.998) (-846.276) [-840.415] (-839.415) * (-851.838) [-841.454] (-850.904) (-843.583) -- 0:03:16 232000 -- (-849.573) [-840.375] (-840.853) (-839.576) * (-845.529) (-851.858) (-842.773) [-840.202] -- 0:03:15 233000 -- (-847.715) (-848.016) (-842.468) [-841.039] * (-837.569) (-843.392) (-839.998) [-846.458] -- 0:03:17 234000 -- (-845.512) (-843.667) (-843.126) [-843.889] * [-841.586] (-841.397) (-849.631) (-853.671) -- 0:03:16 235000 -- (-843.670) (-845.111) (-838.374) [-844.258] * [-840.967] (-835.650) (-846.614) (-843.247) -- 0:03:15 Average standard deviation of split frequencies: 0.016343 236000 -- (-847.592) (-837.727) [-840.314] (-852.293) * (-853.211) [-842.509] (-837.495) (-844.198) -- 0:03:14 237000 -- [-840.027] (-840.157) (-847.937) (-845.410) * [-849.098] (-839.522) (-842.475) (-839.658) -- 0:03:16 238000 -- [-837.401] (-847.815) (-844.019) (-843.690) * [-839.309] (-836.778) (-843.508) (-846.954) -- 0:03:15 239000 -- (-850.766) [-841.105] (-847.738) (-838.609) * (-845.607) [-843.896] (-842.849) (-849.983) -- 0:03:14 240000 -- (-843.338) (-848.710) [-836.035] (-839.485) * [-838.313] (-849.840) (-846.356) (-838.687) -- 0:03:13 Average standard deviation of split frequencies: 0.016204 241000 -- [-845.733] (-837.940) (-839.894) (-852.730) * [-849.815] (-842.064) (-842.035) (-854.682) -- 0:03:15 242000 -- (-839.050) (-843.528) [-839.591] (-840.143) * [-844.489] (-841.503) (-841.477) (-846.327) -- 0:03:14 243000 -- (-845.042) [-838.643] (-851.448) (-840.046) * [-850.174] (-850.892) (-846.110) (-839.977) -- 0:03:13 244000 -- (-845.128) (-846.132) (-848.558) [-841.505] * (-848.276) [-842.510] (-854.825) (-844.195) -- 0:03:12 245000 -- (-854.924) [-841.605] (-856.053) (-843.451) * (-845.065) (-842.508) (-844.723) [-841.450] -- 0:03:14 Average standard deviation of split frequencies: 0.014808 246000 -- (-855.736) [-845.029] (-846.127) (-844.110) * (-845.011) (-841.586) (-848.182) [-843.303] -- 0:03:13 247000 -- (-853.179) (-842.820) (-849.660) [-839.000] * (-841.186) (-847.102) [-837.200] (-841.719) -- 0:03:12 248000 -- [-845.442] (-849.054) (-852.406) (-846.552) * (-847.403) [-839.477] (-846.835) (-847.566) -- 0:03:11 249000 -- (-848.376) (-841.352) (-836.517) [-839.189] * [-843.034] (-853.220) (-845.604) (-849.361) -- 0:03:13 250000 -- (-846.832) [-841.429] (-840.501) (-835.673) * (-843.795) (-838.764) (-846.566) [-844.393] -- 0:03:12 Average standard deviation of split frequencies: 0.013335 251000 -- (-839.627) (-852.578) (-850.027) [-845.344] * (-841.156) (-845.429) (-842.308) [-843.402] -- 0:03:10 252000 -- [-844.276] (-847.574) (-853.578) (-850.097) * (-838.851) (-852.182) [-841.534] (-836.843) -- 0:03:09 253000 -- [-838.123] (-850.128) (-846.080) (-841.422) * (-845.638) (-844.371) (-851.863) [-838.843] -- 0:03:11 254000 -- [-840.678] (-846.433) (-853.148) (-844.858) * (-848.308) [-845.427] (-846.535) (-842.412) -- 0:03:10 255000 -- (-844.651) (-845.832) (-853.462) [-836.899] * [-844.965] (-850.337) (-848.260) (-842.942) -- 0:03:09 Average standard deviation of split frequencies: 0.012220 256000 -- (-846.189) (-840.524) (-842.876) [-843.560] * (-854.802) (-839.719) (-853.661) [-842.581] -- 0:03:08 257000 -- (-849.076) [-849.359] (-843.318) (-844.522) * (-847.489) [-834.991] (-848.025) (-843.013) -- 0:03:10 258000 -- (-841.044) (-847.245) (-848.630) [-842.243] * (-844.451) (-846.500) (-847.338) [-846.495] -- 0:03:09 259000 -- [-836.326] (-844.721) (-848.836) (-847.709) * (-845.801) (-850.503) [-844.519] (-847.935) -- 0:03:08 260000 -- (-843.658) (-847.682) (-846.740) [-840.354] * (-843.872) (-845.548) (-849.712) [-848.944] -- 0:03:07 Average standard deviation of split frequencies: 0.011837 261000 -- (-853.403) [-843.182] (-847.176) (-849.410) * [-845.067] (-853.550) (-841.622) (-844.640) -- 0:03:09 262000 -- (-848.895) (-851.508) [-838.912] (-845.111) * [-841.730] (-855.681) (-849.641) (-853.138) -- 0:03:08 263000 -- [-851.736] (-847.697) (-845.728) (-844.055) * (-850.419) (-842.319) (-849.063) [-841.562] -- 0:03:07 264000 -- (-851.922) [-842.950] (-839.578) (-846.720) * (-852.912) (-843.900) (-850.409) [-843.381] -- 0:03:06 265000 -- (-848.361) [-847.427] (-845.026) (-841.263) * (-846.162) [-841.842] (-845.774) (-850.401) -- 0:03:08 Average standard deviation of split frequencies: 0.012244 266000 -- [-843.054] (-854.528) (-848.350) (-846.557) * (-849.033) [-839.806] (-842.416) (-856.043) -- 0:03:07 267000 -- (-846.160) (-846.894) (-853.165) [-843.227] * (-847.568) [-842.048] (-844.638) (-849.862) -- 0:03:06 268000 -- (-840.653) (-844.449) [-842.419] (-848.169) * [-846.275] (-849.007) (-847.997) (-858.457) -- 0:03:05 269000 -- (-850.450) (-839.241) (-843.107) [-843.550] * (-848.274) [-841.934] (-855.256) (-849.571) -- 0:03:07 270000 -- (-848.684) (-836.997) (-847.307) [-842.614] * [-843.707] (-842.226) (-848.471) (-845.928) -- 0:03:06 Average standard deviation of split frequencies: 0.011717 271000 -- (-853.752) [-846.499] (-847.765) (-849.551) * (-849.964) [-847.532] (-841.450) (-841.981) -- 0:03:05 272000 -- [-851.090] (-846.472) (-849.537) (-848.905) * [-840.194] (-842.574) (-849.851) (-842.873) -- 0:03:07 273000 -- (-853.426) (-849.648) [-840.117] (-841.307) * [-843.400] (-847.559) (-848.660) (-843.097) -- 0:03:06 274000 -- [-851.767] (-849.098) (-845.929) (-851.095) * [-844.010] (-850.262) (-849.628) (-845.747) -- 0:03:05 275000 -- (-844.870) [-847.759] (-848.899) (-845.375) * (-844.122) (-843.467) (-847.867) [-841.177] -- 0:03:04 Average standard deviation of split frequencies: 0.012266 276000 -- (-845.106) [-847.020] (-851.891) (-853.540) * (-845.999) (-846.055) (-852.876) [-842.344] -- 0:03:06 277000 -- (-849.996) (-848.713) [-852.620] (-849.711) * (-842.158) (-841.040) (-841.891) [-848.248] -- 0:03:05 278000 -- (-846.510) [-838.164] (-861.879) (-843.252) * [-845.501] (-848.042) (-844.921) (-839.008) -- 0:03:04 279000 -- [-846.057] (-841.951) (-845.870) (-840.037) * (-844.218) (-850.981) (-842.729) [-838.756] -- 0:03:03 280000 -- (-837.678) (-847.661) (-848.762) [-841.100] * (-841.312) (-845.242) (-849.956) [-840.936] -- 0:03:05 Average standard deviation of split frequencies: 0.009925 281000 -- (-842.944) (-849.496) (-849.428) [-843.054] * (-851.470) (-848.859) [-843.387] (-847.496) -- 0:03:04 282000 -- [-841.556] (-840.486) (-856.957) (-850.722) * (-839.969) (-855.627) [-841.688] (-839.142) -- 0:03:03 283000 -- (-847.934) (-842.684) [-843.586] (-849.810) * [-842.187] (-845.646) (-844.429) (-842.726) -- 0:03:02 284000 -- (-843.571) [-841.616] (-846.718) (-852.428) * (-847.928) [-842.017] (-841.540) (-846.676) -- 0:03:04 285000 -- (-852.438) [-842.808] (-839.077) (-851.598) * (-845.238) [-841.317] (-850.903) (-845.858) -- 0:03:03 Average standard deviation of split frequencies: 0.010939 286000 -- (-847.737) (-847.995) (-844.487) [-837.692] * (-848.503) [-839.978] (-842.831) (-853.834) -- 0:03:02 287000 -- (-842.466) [-851.214] (-843.024) (-847.268) * [-846.382] (-854.130) (-840.111) (-850.315) -- 0:03:01 288000 -- (-841.779) (-840.562) (-844.270) [-849.412] * (-847.886) [-853.303] (-852.653) (-848.853) -- 0:03:02 289000 -- (-849.071) [-844.825] (-851.867) (-846.576) * [-838.999] (-845.437) (-845.672) (-849.075) -- 0:03:02 290000 -- (-846.457) (-842.155) [-840.572] (-857.424) * (-854.097) (-842.080) [-842.188] (-843.040) -- 0:03:01 Average standard deviation of split frequencies: 0.011353 291000 -- (-841.345) (-842.328) [-846.465] (-846.483) * (-849.367) (-846.805) (-846.186) [-845.731] -- 0:03:00 292000 -- (-845.827) (-847.785) (-840.718) [-846.597] * [-843.374] (-845.170) (-843.412) (-843.072) -- 0:03:01 293000 -- [-847.422] (-845.796) (-844.367) (-845.666) * (-842.112) [-840.782] (-845.106) (-845.629) -- 0:03:00 294000 -- (-849.849) (-846.742) [-847.994] (-851.117) * (-842.786) (-839.787) [-846.847] (-853.436) -- 0:03:00 295000 -- (-854.466) (-845.868) (-847.216) [-841.151] * [-845.276] (-837.522) (-848.823) (-861.297) -- 0:02:59 Average standard deviation of split frequencies: 0.011582 296000 -- [-839.399] (-847.758) (-844.902) (-855.337) * (-851.674) (-840.599) [-841.349] (-852.301) -- 0:03:00 297000 -- [-845.264] (-841.263) (-843.335) (-842.354) * (-858.724) (-842.918) [-843.136] (-853.457) -- 0:02:59 298000 -- (-840.611) [-842.982] (-845.261) (-850.605) * (-847.808) [-839.556] (-839.357) (-847.803) -- 0:02:59 299000 -- (-844.759) (-848.060) [-845.438] (-848.703) * (-856.898) [-838.894] (-850.998) (-855.839) -- 0:02:58 300000 -- [-838.385] (-851.296) (-849.817) (-846.539) * (-849.317) (-845.587) [-840.790] (-849.906) -- 0:02:59 Average standard deviation of split frequencies: 0.010833 301000 -- (-851.551) (-843.964) [-841.100] (-837.717) * (-853.912) (-853.010) [-847.480] (-843.495) -- 0:02:58 302000 -- [-847.756] (-842.439) (-841.936) (-852.589) * (-848.637) (-854.398) (-849.935) [-841.002] -- 0:02:57 303000 -- [-844.955] (-856.773) (-841.439) (-845.350) * (-838.789) (-842.402) [-846.656] (-840.502) -- 0:02:57 304000 -- [-844.318] (-847.850) (-857.648) (-846.794) * (-843.264) (-851.564) [-846.277] (-843.776) -- 0:02:58 305000 -- (-852.794) [-848.569] (-842.411) (-841.936) * (-841.908) (-848.578) [-844.661] (-843.389) -- 0:02:57 Average standard deviation of split frequencies: 0.011064 306000 -- (-842.783) (-843.673) (-841.223) [-844.736] * (-852.315) [-840.262] (-838.500) (-842.809) -- 0:02:56 307000 -- (-839.691) [-846.260] (-840.986) (-855.969) * (-861.027) (-849.930) [-840.241] (-838.046) -- 0:02:56 308000 -- [-845.392] (-844.637) (-839.776) (-843.574) * (-857.504) (-845.234) [-842.096] (-838.502) -- 0:02:57 309000 -- [-842.528] (-841.681) (-847.536) (-849.767) * (-848.967) (-840.355) (-847.170) [-843.690] -- 0:02:56 310000 -- (-843.693) [-836.328] (-851.826) (-841.668) * (-850.800) [-841.556] (-841.249) (-839.961) -- 0:02:55 Average standard deviation of split frequencies: 0.011174 311000 -- (-835.674) [-839.762] (-853.041) (-838.057) * [-842.909] (-851.821) (-840.388) (-844.201) -- 0:02:55 312000 -- (-845.484) (-846.337) (-850.402) [-842.693] * (-852.108) (-843.149) [-841.000] (-846.305) -- 0:02:56 313000 -- (-845.770) [-848.732] (-849.044) (-845.875) * (-843.238) (-846.419) [-847.421] (-856.570) -- 0:02:55 314000 -- (-852.525) (-844.301) [-843.875] (-854.987) * [-847.600] (-846.418) (-847.004) (-853.533) -- 0:02:54 315000 -- [-838.726] (-842.241) (-844.737) (-846.234) * (-850.536) (-846.678) (-839.922) [-852.146] -- 0:02:53 Average standard deviation of split frequencies: 0.010307 316000 -- (-846.563) (-851.077) (-845.879) [-845.860] * (-845.153) [-840.670] (-849.172) (-850.578) -- 0:02:55 317000 -- (-850.163) [-839.408] (-847.405) (-847.055) * (-840.875) [-836.049] (-843.069) (-844.111) -- 0:02:54 318000 -- (-848.200) [-838.045] (-839.068) (-844.888) * (-839.249) [-842.738] (-840.514) (-845.730) -- 0:02:53 319000 -- (-852.624) (-839.942) [-843.422] (-850.043) * (-838.636) [-846.702] (-849.842) (-838.667) -- 0:02:52 320000 -- (-843.470) [-840.727] (-843.798) (-840.398) * [-838.191] (-844.327) (-839.943) (-851.686) -- 0:02:54 Average standard deviation of split frequencies: 0.010825 321000 -- [-846.638] (-844.047) (-843.538) (-846.861) * (-853.809) (-845.293) (-839.174) [-838.737] -- 0:02:53 322000 -- (-853.293) [-839.987] (-851.345) (-837.219) * (-845.704) (-861.174) (-841.189) [-839.483] -- 0:02:52 323000 -- (-861.551) (-845.919) (-845.847) [-840.209] * (-845.930) [-849.178] (-847.464) (-844.884) -- 0:02:51 324000 -- (-851.527) (-841.679) [-845.801] (-847.096) * (-858.599) [-839.257] (-841.304) (-846.088) -- 0:02:53 325000 -- (-837.020) [-839.346] (-845.328) (-844.950) * (-847.343) (-845.992) (-839.612) [-844.460] -- 0:02:52 Average standard deviation of split frequencies: 0.010911 326000 -- (-851.306) (-842.247) (-839.601) [-843.400] * (-849.785) [-845.493] (-853.696) (-843.835) -- 0:02:51 327000 -- (-849.268) (-845.024) [-846.605] (-841.049) * (-859.576) (-846.200) (-848.812) [-836.319] -- 0:02:50 328000 -- (-858.736) (-846.902) (-844.314) [-846.795] * (-842.597) (-838.715) (-845.760) [-844.735] -- 0:02:52 329000 -- (-853.334) (-838.085) [-837.069] (-839.579) * (-851.632) (-847.164) [-848.774] (-842.428) -- 0:02:51 330000 -- (-839.594) (-842.156) [-840.983] (-849.392) * (-858.676) (-846.249) (-843.614) [-843.103] -- 0:02:50 Average standard deviation of split frequencies: 0.008295 331000 -- (-849.591) [-842.055] (-852.346) (-841.508) * (-841.488) [-838.943] (-845.744) (-843.506) -- 0:02:49 332000 -- (-844.258) (-843.006) [-843.708] (-847.955) * (-839.591) [-843.620] (-851.020) (-852.104) -- 0:02:51 333000 -- (-848.659) [-845.396] (-844.368) (-846.924) * [-841.997] (-854.584) (-841.375) (-847.885) -- 0:02:50 334000 -- [-854.365] (-840.379) (-846.001) (-842.198) * (-838.876) (-846.136) [-841.366] (-843.566) -- 0:02:49 335000 -- (-840.259) [-842.984] (-848.939) (-842.770) * (-838.542) [-841.157] (-843.189) (-843.635) -- 0:02:50 Average standard deviation of split frequencies: 0.007908 336000 -- (-852.225) (-843.150) (-848.889) [-846.104] * (-842.590) (-846.219) (-840.143) [-847.014] -- 0:02:49 337000 -- (-845.380) (-842.966) (-841.667) [-843.122] * (-842.166) (-845.614) [-844.049] (-856.679) -- 0:02:49 338000 -- [-842.705] (-864.303) (-838.759) (-854.550) * (-842.209) (-849.666) [-848.221] (-848.570) -- 0:02:48 339000 -- (-842.229) (-845.623) [-844.585] (-847.344) * [-840.862] (-845.693) (-839.829) (-851.024) -- 0:02:49 340000 -- (-843.773) [-845.417] (-846.741) (-848.480) * (-846.127) (-842.091) (-849.133) [-845.952] -- 0:02:48 Average standard deviation of split frequencies: 0.007422 341000 -- (-838.359) [-843.277] (-849.596) (-848.176) * [-850.549] (-841.802) (-855.319) (-840.478) -- 0:02:48 342000 -- (-846.023) [-848.514] (-842.420) (-845.841) * (-842.077) [-837.837] (-841.286) (-845.497) -- 0:02:47 343000 -- (-841.200) [-845.180] (-844.752) (-845.791) * (-845.559) [-847.220] (-847.403) (-853.309) -- 0:02:48 344000 -- (-842.398) [-838.472] (-847.031) (-851.405) * [-839.087] (-847.649) (-840.646) (-848.516) -- 0:02:47 345000 -- (-847.875) (-838.465) (-851.759) [-847.586] * (-842.112) [-840.711] (-847.946) (-844.204) -- 0:02:47 Average standard deviation of split frequencies: 0.009042 346000 -- (-846.108) [-847.250] (-843.229) (-850.353) * (-850.225) (-846.490) (-844.645) [-846.108] -- 0:02:46 347000 -- (-848.105) (-858.740) [-840.057] (-840.538) * [-850.362] (-847.354) (-840.938) (-846.823) -- 0:02:47 348000 -- (-848.737) (-849.834) (-842.949) [-843.511] * (-845.566) (-847.075) (-846.016) [-844.710] -- 0:02:46 349000 -- (-852.712) (-841.478) (-842.986) [-836.028] * (-843.377) [-847.452] (-842.458) (-848.703) -- 0:02:46 350000 -- (-860.261) [-840.351] (-849.579) (-844.109) * (-844.484) [-846.962] (-848.514) (-848.117) -- 0:02:45 Average standard deviation of split frequencies: 0.009532 351000 -- (-852.638) (-842.622) (-846.443) [-842.353] * [-840.223] (-845.673) (-844.898) (-840.433) -- 0:02:46 352000 -- (-843.386) (-844.389) (-851.666) [-843.543] * (-845.739) [-840.317] (-848.369) (-845.720) -- 0:02:45 353000 -- [-842.530] (-841.784) (-847.586) (-845.970) * (-846.236) [-843.629] (-841.635) (-853.611) -- 0:02:44 354000 -- (-850.366) [-840.814] (-852.695) (-846.513) * (-844.782) [-844.143] (-847.607) (-845.884) -- 0:02:44 355000 -- (-853.130) (-841.101) [-846.011] (-852.858) * (-847.857) (-838.693) (-845.136) [-842.085] -- 0:02:45 Average standard deviation of split frequencies: 0.009028 356000 -- [-844.419] (-849.755) (-856.347) (-843.030) * (-847.567) (-842.195) (-848.918) [-850.161] -- 0:02:44 357000 -- [-841.081] (-849.129) (-845.812) (-846.470) * [-846.168] (-846.652) (-844.276) (-851.991) -- 0:02:43 358000 -- (-849.766) (-841.244) (-847.796) [-836.896] * [-846.176] (-843.463) (-846.424) (-844.345) -- 0:02:43 359000 -- (-841.447) (-840.411) [-836.205] (-852.400) * [-843.755] (-843.441) (-840.860) (-838.999) -- 0:02:44 360000 -- (-844.338) (-842.510) (-848.618) [-841.187] * (-844.320) (-847.636) [-846.537] (-843.384) -- 0:02:43 Average standard deviation of split frequencies: 0.008793 361000 -- (-836.954) (-839.506) [-840.734] (-851.758) * (-849.879) [-850.440] (-842.823) (-849.402) -- 0:02:42 362000 -- (-851.503) [-843.118] (-847.542) (-845.972) * (-847.767) (-841.405) [-841.850] (-850.584) -- 0:02:42 363000 -- (-840.067) [-845.210] (-847.410) (-841.369) * (-849.543) (-847.101) (-844.564) [-846.089] -- 0:02:43 364000 -- (-848.325) (-849.585) (-840.520) [-844.503] * [-851.732] (-858.844) (-848.164) (-846.650) -- 0:02:42 365000 -- (-851.001) (-848.125) [-849.144] (-851.934) * (-846.757) [-848.359] (-844.150) (-841.849) -- 0:02:41 Average standard deviation of split frequencies: 0.008313 366000 -- (-847.213) [-840.169] (-847.826) (-842.463) * (-852.290) (-854.968) [-847.075] (-841.831) -- 0:02:41 367000 -- (-847.618) (-847.260) [-839.957] (-847.108) * [-849.287] (-842.586) (-847.426) (-842.924) -- 0:02:42 368000 -- (-846.374) (-854.221) [-842.282] (-838.877) * [-847.709] (-845.289) (-845.005) (-842.996) -- 0:02:41 369000 -- (-847.705) (-848.163) (-838.731) [-845.343] * [-840.600] (-845.234) (-848.610) (-857.096) -- 0:02:40 370000 -- (-842.508) (-846.585) [-838.593] (-845.909) * (-841.744) [-842.842] (-849.990) (-847.260) -- 0:02:40 Average standard deviation of split frequencies: 0.008093 371000 -- [-844.550] (-839.796) (-846.692) (-841.776) * (-841.954) [-846.470] (-849.140) (-845.044) -- 0:02:41 372000 -- (-854.225) (-841.616) (-842.289) [-841.457] * [-839.738] (-849.846) (-843.241) (-857.441) -- 0:02:40 373000 -- (-847.828) (-840.604) (-856.513) [-837.980] * [-839.548] (-849.375) (-845.367) (-847.332) -- 0:02:39 374000 -- [-844.328] (-838.937) (-855.627) (-837.914) * [-841.300] (-850.552) (-847.640) (-861.787) -- 0:02:39 375000 -- (-851.425) (-856.485) (-842.287) [-846.865] * (-842.079) (-851.949) (-852.532) [-849.267] -- 0:02:40 Average standard deviation of split frequencies: 0.008662 376000 -- (-849.302) (-842.756) (-840.600) [-843.275] * (-846.451) [-848.286] (-848.028) (-844.421) -- 0:02:39 377000 -- (-845.225) (-846.435) [-848.042] (-848.834) * (-843.692) (-849.006) [-839.352] (-846.471) -- 0:02:38 378000 -- (-846.829) [-842.449] (-844.170) (-847.575) * (-845.721) [-844.072] (-853.226) (-842.980) -- 0:02:37 379000 -- (-846.391) (-840.536) (-844.030) [-839.590] * [-840.983] (-855.215) (-847.799) (-846.365) -- 0:02:38 380000 -- (-840.276) (-845.161) [-845.476] (-843.035) * [-844.601] (-851.136) (-853.532) (-841.447) -- 0:02:38 Average standard deviation of split frequencies: 0.009569 381000 -- [-842.364] (-847.262) (-844.921) (-841.138) * (-850.577) (-843.981) (-848.468) [-842.165] -- 0:02:37 382000 -- (-841.136) [-842.100] (-847.939) (-851.919) * (-846.046) (-843.014) (-840.071) [-844.710] -- 0:02:36 383000 -- (-847.512) (-843.353) [-840.809] (-851.875) * (-847.559) (-839.984) [-844.233] (-852.378) -- 0:02:37 384000 -- (-844.286) (-852.319) (-849.310) [-839.993] * (-852.912) (-847.568) [-843.082] (-848.715) -- 0:02:37 385000 -- (-846.710) (-851.614) [-838.843] (-852.075) * [-847.779] (-850.547) (-837.812) (-843.177) -- 0:02:36 Average standard deviation of split frequencies: 0.009215 386000 -- (-837.618) (-855.704) (-840.434) [-840.389] * [-843.812] (-843.617) (-839.189) (-848.463) -- 0:02:35 387000 -- (-840.311) [-842.938] (-848.560) (-845.229) * [-843.679] (-846.558) (-838.293) (-844.171) -- 0:02:36 388000 -- (-843.021) (-846.086) (-842.258) [-842.948] * (-843.709) [-841.802] (-846.925) (-845.902) -- 0:02:36 389000 -- (-854.141) (-841.532) (-858.460) [-844.929] * [-841.415] (-855.576) (-839.316) (-844.678) -- 0:02:35 390000 -- (-842.556) (-848.015) (-842.149) [-841.450] * (-847.900) (-839.137) [-840.645] (-841.522) -- 0:02:34 Average standard deviation of split frequencies: 0.009434 391000 -- [-842.756] (-844.909) (-851.069) (-847.520) * (-849.731) (-840.790) (-849.222) [-841.747] -- 0:02:35 392000 -- [-845.235] (-852.634) (-839.897) (-849.815) * (-843.581) (-847.415) [-842.285] (-851.997) -- 0:02:35 393000 -- (-848.858) (-846.003) [-845.092] (-847.095) * [-839.447] (-844.288) (-851.637) (-845.029) -- 0:02:34 394000 -- (-849.670) (-847.715) (-841.499) [-843.810] * (-843.320) (-845.890) [-844.093] (-847.820) -- 0:02:33 395000 -- (-842.888) [-840.925] (-843.075) (-847.635) * (-839.136) [-841.888] (-845.171) (-844.289) -- 0:02:34 Average standard deviation of split frequencies: 0.008658 396000 -- (-846.901) [-839.930] (-847.129) (-837.573) * [-844.690] (-847.246) (-848.831) (-844.892) -- 0:02:34 397000 -- (-850.735) [-843.081] (-853.116) (-843.673) * (-851.974) (-841.171) [-839.225] (-845.342) -- 0:02:33 398000 -- (-839.964) [-844.153] (-844.708) (-842.884) * [-841.369] (-847.320) (-840.111) (-835.839) -- 0:02:32 399000 -- (-843.928) (-845.169) [-842.409] (-839.451) * (-853.278) (-847.987) [-844.558] (-843.608) -- 0:02:33 400000 -- (-839.909) (-847.777) [-837.648] (-848.872) * (-844.960) (-846.343) [-838.427] (-850.541) -- 0:02:33 Average standard deviation of split frequencies: 0.008022 401000 -- (-842.537) [-846.630] (-843.011) (-842.942) * [-846.615] (-842.428) (-844.494) (-845.172) -- 0:02:32 402000 -- (-843.773) [-842.030] (-839.383) (-848.567) * (-845.804) (-854.726) [-838.439] (-839.423) -- 0:02:31 403000 -- (-843.582) (-841.823) [-840.546] (-838.291) * (-845.174) (-843.400) (-835.704) [-840.065] -- 0:02:32 404000 -- (-848.125) [-841.957] (-842.489) (-852.440) * (-843.750) (-845.922) [-848.935] (-842.623) -- 0:02:31 405000 -- [-842.838] (-843.265) (-843.414) (-841.543) * (-844.071) (-841.698) (-849.364) [-845.730] -- 0:02:31 Average standard deviation of split frequencies: 0.008867 406000 -- [-849.686] (-848.086) (-846.012) (-845.568) * (-839.945) [-848.884] (-846.545) (-844.636) -- 0:02:30 407000 -- (-845.704) [-841.038] (-845.283) (-848.398) * [-844.355] (-838.855) (-838.820) (-846.850) -- 0:02:31 408000 -- (-840.680) [-842.239] (-843.508) (-845.470) * [-844.297] (-840.237) (-842.706) (-841.596) -- 0:02:30 409000 -- (-850.975) (-845.796) (-843.984) [-849.776] * (-845.424) [-850.871] (-844.744) (-845.203) -- 0:02:30 410000 -- [-843.786] (-839.731) (-842.078) (-841.160) * (-843.866) [-840.009] (-844.261) (-848.238) -- 0:02:29 Average standard deviation of split frequencies: 0.008870 411000 -- (-849.736) (-846.318) [-847.904] (-854.561) * (-845.589) (-855.717) [-841.809] (-845.087) -- 0:02:30 412000 -- (-843.681) (-842.762) [-846.319] (-839.767) * (-844.299) [-834.574] (-850.522) (-845.520) -- 0:02:29 413000 -- [-843.782] (-849.080) (-862.277) (-839.978) * [-841.998] (-842.006) (-847.919) (-845.508) -- 0:02:29 414000 -- (-853.952) (-845.579) [-840.553] (-848.357) * (-845.651) [-840.361] (-846.779) (-844.403) -- 0:02:30 415000 -- (-843.383) (-848.056) [-844.177] (-841.622) * (-841.521) (-840.910) (-842.399) [-839.320] -- 0:02:29 Average standard deviation of split frequencies: 0.008962 416000 -- (-850.526) (-844.024) [-842.354] (-850.527) * (-836.562) [-845.256] (-841.964) (-843.119) -- 0:02:28 417000 -- [-841.468] (-842.089) (-848.127) (-840.777) * (-841.692) (-843.323) (-853.711) [-841.910] -- 0:02:28 418000 -- (-849.613) (-844.268) [-844.224] (-839.324) * (-852.923) (-842.718) (-837.694) [-844.914] -- 0:02:28 419000 -- (-860.881) (-839.665) (-843.443) [-838.465] * (-844.871) (-843.244) [-842.679] (-851.015) -- 0:02:28 420000 -- [-848.651] (-840.527) (-845.768) (-850.901) * [-847.341] (-850.621) (-844.283) (-853.964) -- 0:02:27 Average standard deviation of split frequencies: 0.007437 421000 -- (-863.431) [-843.462] (-859.693) (-843.173) * (-841.725) (-851.917) [-843.054] (-843.654) -- 0:02:27 422000 -- (-850.957) (-847.439) (-840.687) [-842.289] * (-839.576) (-846.326) (-843.817) [-845.867] -- 0:02:27 423000 -- (-848.020) (-845.585) [-840.619] (-844.363) * (-843.356) [-850.093] (-851.294) (-848.323) -- 0:02:27 424000 -- (-843.274) [-840.754] (-845.050) (-846.250) * (-841.812) (-849.700) [-844.608] (-846.772) -- 0:02:26 425000 -- (-840.428) (-845.380) (-858.007) [-842.610] * (-851.112) [-854.492] (-838.582) (-851.438) -- 0:02:26 Average standard deviation of split frequencies: 0.007847 426000 -- [-837.414] (-844.655) (-842.225) (-849.976) * [-839.328] (-845.930) (-844.936) (-844.759) -- 0:02:26 427000 -- (-844.420) (-841.919) [-842.253] (-850.804) * (-845.214) [-845.353] (-841.350) (-842.268) -- 0:02:26 428000 -- (-843.400) [-844.046] (-844.074) (-842.343) * (-853.742) [-843.553] (-842.438) (-844.062) -- 0:02:25 429000 -- (-841.693) (-845.998) (-847.004) [-844.432] * (-850.152) (-845.964) [-838.234] (-843.171) -- 0:02:25 430000 -- [-840.512] (-845.487) (-848.981) (-845.324) * (-847.553) (-853.902) [-842.159] (-837.594) -- 0:02:25 Average standard deviation of split frequencies: 0.008259 431000 -- (-840.372) [-840.768] (-854.344) (-849.418) * [-845.046] (-844.705) (-856.658) (-841.795) -- 0:02:25 432000 -- (-845.513) (-850.453) (-848.208) [-849.012] * (-843.040) [-842.913] (-850.940) (-841.385) -- 0:02:24 433000 -- (-841.990) (-848.307) (-843.680) [-841.234] * (-847.958) (-839.557) (-843.396) [-842.519] -- 0:02:24 434000 -- (-847.601) [-846.461] (-840.432) (-841.136) * (-848.356) (-844.868) [-841.095] (-850.098) -- 0:02:24 435000 -- (-843.685) (-849.091) (-842.893) [-850.986] * (-840.743) (-844.245) (-842.662) [-845.503] -- 0:02:24 Average standard deviation of split frequencies: 0.007372 436000 -- [-839.103] (-842.336) (-845.949) (-847.392) * [-838.938] (-843.936) (-845.440) (-840.997) -- 0:02:23 437000 -- (-839.626) [-859.621] (-842.653) (-840.195) * (-845.195) [-842.854] (-847.021) (-844.053) -- 0:02:23 438000 -- [-841.658] (-846.093) (-845.888) (-849.650) * (-847.751) (-854.334) (-854.191) [-846.813] -- 0:02:23 439000 -- (-855.821) [-848.936] (-840.354) (-847.389) * (-843.080) [-843.178] (-849.140) (-839.911) -- 0:02:23 440000 -- (-846.058) (-846.498) [-841.662] (-837.933) * (-847.983) (-844.326) [-845.193] (-844.229) -- 0:02:22 Average standard deviation of split frequencies: 0.006321 441000 -- (-851.908) (-842.647) (-844.723) [-843.304] * (-838.264) (-851.350) [-845.471] (-847.899) -- 0:02:21 442000 -- [-848.019] (-841.723) (-843.665) (-850.486) * (-850.464) (-846.061) (-851.658) [-839.207] -- 0:02:22 443000 -- (-846.223) (-847.744) (-845.865) [-849.319] * (-846.007) (-838.896) (-852.685) [-850.432] -- 0:02:22 444000 -- (-840.373) [-840.656] (-851.775) (-843.301) * [-843.979] (-839.945) (-847.824) (-845.288) -- 0:02:21 445000 -- (-843.869) [-842.118] (-844.363) (-843.053) * (-851.224) (-845.374) [-844.348] (-845.077) -- 0:02:20 Average standard deviation of split frequencies: 0.006438 446000 -- [-842.395] (-843.923) (-843.966) (-846.846) * (-842.011) (-843.007) (-848.114) [-843.137] -- 0:02:21 447000 -- [-840.167] (-850.808) (-849.804) (-847.693) * (-845.966) (-837.374) (-842.861) [-839.233] -- 0:02:21 448000 -- [-846.865] (-848.039) (-838.875) (-843.978) * (-858.955) (-843.601) (-842.895) [-846.956] -- 0:02:20 449000 -- (-839.629) [-842.238] (-850.551) (-840.940) * (-840.539) (-843.908) (-846.888) [-840.508] -- 0:02:19 450000 -- (-848.460) [-848.833] (-843.497) (-840.351) * [-838.706] (-847.459) (-837.737) (-843.553) -- 0:02:20 Average standard deviation of split frequencies: 0.008273 451000 -- (-847.346) [-842.036] (-843.249) (-841.363) * [-837.835] (-841.726) (-852.970) (-844.857) -- 0:02:19 452000 -- [-848.970] (-844.299) (-852.624) (-843.075) * (-841.033) (-847.611) (-845.558) [-842.419] -- 0:02:19 453000 -- [-837.663] (-839.492) (-848.692) (-843.042) * (-839.533) (-841.994) (-842.777) [-842.627] -- 0:02:18 454000 -- [-844.728] (-843.358) (-844.018) (-853.334) * (-842.140) (-835.879) (-841.082) [-838.866] -- 0:02:19 455000 -- (-844.613) [-843.611] (-850.998) (-851.419) * (-850.563) (-847.991) (-842.233) [-844.982] -- 0:02:18 Average standard deviation of split frequencies: 0.007988 456000 -- (-846.793) [-836.950] (-844.044) (-848.036) * (-844.915) [-853.674] (-846.670) (-841.276) -- 0:02:18 457000 -- (-848.889) [-844.167] (-845.427) (-845.362) * [-845.254] (-845.639) (-841.422) (-850.191) -- 0:02:19 458000 -- [-847.506] (-840.528) (-854.505) (-848.155) * [-841.278] (-845.631) (-842.126) (-850.216) -- 0:02:18 459000 -- (-852.712) (-844.525) (-841.825) [-853.246] * [-840.948] (-854.840) (-844.695) (-842.637) -- 0:02:17 460000 -- (-853.091) (-847.015) [-841.265] (-849.517) * (-849.137) [-840.014] (-845.156) (-843.616) -- 0:02:17 Average standard deviation of split frequencies: 0.008652 461000 -- (-860.283) (-842.501) [-843.805] (-850.727) * (-847.218) [-838.882] (-847.978) (-836.425) -- 0:02:17 462000 -- (-850.602) [-840.046] (-846.803) (-845.455) * [-841.671] (-844.662) (-842.240) (-853.165) -- 0:02:17 463000 -- [-842.805] (-840.703) (-851.132) (-855.003) * (-847.270) (-841.318) [-848.461] (-846.500) -- 0:02:16 464000 -- (-842.256) (-843.666) (-842.177) [-841.430] * (-845.674) [-845.128] (-843.787) (-843.027) -- 0:02:16 465000 -- (-855.210) (-854.190) [-845.514] (-847.014) * (-843.761) (-841.911) (-848.486) [-834.486] -- 0:02:16 Average standard deviation of split frequencies: 0.009472 466000 -- (-837.909) (-845.694) [-840.777] (-847.349) * (-852.036) [-847.754] (-847.978) (-837.739) -- 0:02:16 467000 -- (-845.030) (-842.455) (-838.003) [-841.287] * [-849.630] (-843.173) (-843.006) (-847.203) -- 0:02:15 468000 -- (-842.476) (-842.837) [-837.095] (-846.728) * (-853.066) [-835.804] (-841.628) (-845.865) -- 0:02:15 469000 -- (-845.482) (-844.151) [-847.519] (-847.269) * (-853.609) (-844.997) [-841.045] (-844.331) -- 0:02:15 470000 -- (-841.590) (-846.495) (-847.384) [-838.394] * (-854.568) (-847.295) [-847.103] (-853.838) -- 0:02:15 Average standard deviation of split frequencies: 0.010107 471000 -- (-836.562) (-846.547) (-851.436) [-842.271] * (-844.149) (-848.157) (-850.515) [-842.054] -- 0:02:14 472000 -- (-850.711) (-852.770) [-851.554] (-846.645) * (-849.773) [-846.160] (-845.726) (-848.007) -- 0:02:14 473000 -- (-848.488) (-844.903) (-842.417) [-844.070] * (-842.367) [-843.891] (-847.690) (-845.529) -- 0:02:14 474000 -- [-846.962] (-843.567) (-848.949) (-842.107) * [-840.937] (-846.780) (-842.859) (-848.844) -- 0:02:14 475000 -- (-838.004) [-841.962] (-845.399) (-846.308) * (-846.344) (-847.950) [-849.558] (-845.160) -- 0:02:13 Average standard deviation of split frequencies: 0.009093 476000 -- [-852.948] (-841.419) (-846.892) (-840.266) * (-852.830) [-841.579] (-846.242) (-847.254) -- 0:02:13 477000 -- (-849.232) (-837.268) (-851.327) [-846.158] * (-849.509) (-844.334) [-845.049] (-860.424) -- 0:02:13 478000 -- (-846.883) (-848.085) [-841.224] (-838.206) * (-850.792) (-837.919) [-839.821] (-845.285) -- 0:02:13 479000 -- (-855.966) (-840.131) [-844.695] (-846.560) * (-844.829) (-845.334) (-841.602) [-844.512] -- 0:02:12 480000 -- (-849.300) (-841.260) [-841.344] (-851.904) * (-843.913) (-843.080) [-839.880] (-843.610) -- 0:02:12 Average standard deviation of split frequencies: 0.009272 481000 -- (-846.804) (-841.693) [-844.654] (-845.113) * (-841.454) (-847.487) (-846.532) [-839.591] -- 0:02:12 482000 -- (-847.881) (-851.966) [-856.563] (-844.410) * (-856.969) [-850.323] (-845.804) (-846.668) -- 0:02:12 483000 -- (-845.523) (-852.856) (-867.342) [-837.057] * (-850.796) (-838.630) [-841.609] (-844.303) -- 0:02:11 484000 -- [-839.845] (-837.583) (-850.705) (-843.287) * (-853.716) [-845.277] (-841.833) (-846.614) -- 0:02:11 485000 -- (-846.895) (-844.358) (-844.558) [-842.665] * [-845.233] (-847.643) (-845.694) (-844.454) -- 0:02:11 Average standard deviation of split frequencies: 0.010141 486000 -- (-842.662) [-843.371] (-840.776) (-841.505) * (-846.215) [-840.896] (-837.145) (-844.802) -- 0:02:11 487000 -- [-839.450] (-844.913) (-856.851) (-848.480) * (-847.850) (-846.707) [-840.319] (-839.237) -- 0:02:10 488000 -- (-841.952) [-846.425] (-850.971) (-849.867) * (-846.867) (-845.260) [-843.891] (-841.615) -- 0:02:10 489000 -- (-856.358) [-841.745] (-848.216) (-843.179) * (-849.767) (-845.923) (-842.604) [-847.320] -- 0:02:10 490000 -- (-841.344) (-845.663) (-841.886) [-840.052] * (-849.893) (-848.865) (-852.499) [-841.256] -- 0:02:10 Average standard deviation of split frequencies: 0.010393 491000 -- (-838.969) (-852.723) (-854.050) [-844.801] * [-851.130] (-847.084) (-842.906) (-846.681) -- 0:02:09 492000 -- (-846.131) [-847.077] (-841.066) (-847.670) * [-846.337] (-843.482) (-846.334) (-842.254) -- 0:02:09 493000 -- (-855.511) (-843.204) (-843.598) [-841.975] * (-849.854) (-847.434) [-846.504] (-842.740) -- 0:02:09 494000 -- [-841.918] (-846.321) (-842.226) (-840.731) * (-844.583) [-842.097] (-847.090) (-841.010) -- 0:02:09 495000 -- [-839.411] (-852.998) (-842.506) (-840.763) * (-848.531) [-846.591] (-847.461) (-850.790) -- 0:02:08 Average standard deviation of split frequencies: 0.010455 496000 -- (-846.296) [-843.036] (-837.426) (-847.344) * (-852.733) [-850.700] (-840.393) (-845.619) -- 0:02:08 497000 -- (-839.131) (-845.590) [-838.731] (-849.197) * (-853.498) (-847.387) (-843.613) [-844.328] -- 0:02:08 498000 -- (-844.463) [-837.311] (-842.280) (-853.414) * (-850.164) (-848.176) (-846.664) [-841.538] -- 0:02:08 499000 -- (-859.150) [-838.197] (-853.738) (-849.506) * (-855.701) (-844.494) [-843.156] (-841.692) -- 0:02:07 500000 -- (-846.521) [-843.809] (-841.204) (-842.607) * [-840.197] (-853.106) (-840.803) (-839.697) -- 0:02:07 Average standard deviation of split frequencies: 0.010100 501000 -- [-847.969] (-844.754) (-852.565) (-839.825) * (-849.625) (-851.744) [-841.541] (-855.618) -- 0:02:07 502000 -- (-838.279) [-843.778] (-847.441) (-845.172) * (-863.045) (-844.929) [-849.514] (-846.670) -- 0:02:06 503000 -- (-844.667) (-844.207) (-843.941) [-848.477] * (-846.302) (-844.572) (-843.177) [-845.309] -- 0:02:06 504000 -- [-846.056] (-843.381) (-840.250) (-848.624) * (-850.697) (-850.930) (-852.310) [-843.645] -- 0:02:05 505000 -- (-851.242) (-846.238) (-843.272) [-841.112] * (-849.871) [-843.553] (-855.406) (-841.535) -- 0:02:06 Average standard deviation of split frequencies: 0.009232 506000 -- (-844.872) (-848.467) (-846.338) [-842.138] * (-840.255) (-840.993) [-842.983] (-848.548) -- 0:02:05 507000 -- (-840.762) (-854.540) (-848.001) [-847.708] * (-841.263) (-849.374) (-844.971) [-839.569] -- 0:02:05 508000 -- [-839.451] (-842.709) (-841.528) (-839.447) * [-842.051] (-852.503) (-851.686) (-843.672) -- 0:02:04 509000 -- [-841.934] (-847.224) (-841.930) (-844.681) * (-840.639) (-843.143) [-847.820] (-846.364) -- 0:02:05 510000 -- (-842.812) [-840.570] (-842.482) (-848.728) * (-848.400) (-843.957) (-846.612) [-841.901] -- 0:02:04 Average standard deviation of split frequencies: 0.008308 511000 -- (-842.865) (-848.352) (-844.520) [-843.291] * [-841.526] (-846.510) (-849.034) (-846.212) -- 0:02:04 512000 -- [-847.574] (-840.054) (-850.027) (-843.823) * [-840.413] (-844.274) (-846.877) (-840.212) -- 0:02:03 513000 -- [-843.283] (-843.635) (-841.330) (-852.242) * (-843.641) (-841.585) [-844.121] (-849.441) -- 0:02:04 514000 -- [-838.842] (-846.021) (-852.963) (-844.921) * (-847.022) (-851.891) [-839.330] (-841.612) -- 0:02:03 515000 -- (-843.210) (-848.575) [-843.815] (-838.016) * (-845.463) [-838.984] (-836.280) (-847.525) -- 0:02:03 Average standard deviation of split frequencies: 0.006312 516000 -- (-846.874) (-843.333) [-847.856] (-845.389) * (-851.610) (-844.599) (-845.798) [-840.753] -- 0:02:02 517000 -- (-851.046) [-846.958] (-844.370) (-849.818) * [-841.842] (-856.341) (-837.151) (-838.786) -- 0:02:03 518000 -- (-845.664) (-846.626) [-841.343] (-851.061) * (-849.706) (-861.779) [-847.672] (-847.014) -- 0:02:02 519000 -- [-844.191] (-846.950) (-848.998) (-849.558) * [-846.145] (-848.001) (-844.299) (-840.961) -- 0:02:02 520000 -- (-841.856) [-848.610] (-844.669) (-837.867) * (-852.509) (-840.764) [-844.173] (-842.939) -- 0:02:01 Average standard deviation of split frequencies: 0.005844 521000 -- (-848.679) [-843.979] (-841.997) (-837.235) * (-846.452) (-846.106) (-847.972) [-840.230] -- 0:02:02 522000 -- [-842.713] (-853.105) (-840.767) (-844.289) * (-844.885) [-842.944] (-846.806) (-842.087) -- 0:02:01 523000 -- (-841.861) [-846.283] (-843.070) (-853.134) * [-846.796] (-850.139) (-840.068) (-841.560) -- 0:02:01 524000 -- (-845.830) [-845.088] (-844.116) (-847.383) * [-844.295] (-847.699) (-843.415) (-841.452) -- 0:02:01 525000 -- (-847.091) (-849.724) (-846.520) [-843.061] * (-849.767) [-841.716] (-850.831) (-852.037) -- 0:02:01 Average standard deviation of split frequencies: 0.006273 526000 -- (-845.058) [-840.493] (-842.951) (-840.149) * (-849.742) (-844.647) [-838.961] (-845.006) -- 0:02:00 527000 -- (-843.980) (-842.652) [-844.496] (-847.974) * (-851.826) [-841.660] (-846.638) (-844.449) -- 0:02:00 528000 -- (-859.831) [-850.529] (-847.887) (-845.597) * (-853.435) [-840.390] (-851.446) (-848.218) -- 0:02:00 529000 -- [-840.371] (-856.725) (-842.246) (-845.520) * (-847.770) [-849.002] (-850.012) (-846.059) -- 0:02:00 530000 -- (-846.397) [-843.949] (-845.618) (-846.914) * [-839.669] (-846.364) (-849.705) (-847.246) -- 0:01:59 Average standard deviation of split frequencies: 0.005734 531000 -- (-840.405) [-842.216] (-841.183) (-847.034) * (-848.042) (-842.534) (-847.605) [-842.313] -- 0:01:59 532000 -- [-842.057] (-841.047) (-850.357) (-850.594) * [-841.477] (-843.096) (-841.025) (-841.875) -- 0:01:59 533000 -- (-849.225) (-843.403) [-849.568] (-855.170) * [-844.234] (-845.766) (-845.140) (-842.664) -- 0:01:59 534000 -- (-841.131) (-843.539) [-841.335] (-850.376) * (-849.568) [-844.164] (-846.124) (-854.359) -- 0:01:58 535000 -- (-841.385) [-842.382] (-841.245) (-851.430) * (-847.576) [-845.477] (-844.609) (-841.719) -- 0:01:58 Average standard deviation of split frequencies: 0.005437 536000 -- (-847.696) [-843.386] (-848.091) (-844.543) * (-846.483) [-847.692] (-842.389) (-848.453) -- 0:01:58 537000 -- (-844.638) (-855.764) (-852.250) [-842.233] * (-841.441) [-843.700] (-850.234) (-846.144) -- 0:01:58 538000 -- (-846.067) (-849.127) [-850.150] (-844.034) * (-843.294) (-851.797) (-840.197) [-840.537] -- 0:01:57 539000 -- [-846.956] (-849.657) (-841.202) (-844.576) * (-849.425) (-840.587) [-844.746] (-857.097) -- 0:01:57 540000 -- (-841.231) (-848.115) (-840.083) [-837.941] * [-844.311] (-844.343) (-838.883) (-839.285) -- 0:01:57 Average standard deviation of split frequencies: 0.005865 541000 -- (-850.905) [-848.416] (-841.293) (-853.166) * (-843.543) [-841.508] (-851.847) (-840.697) -- 0:01:57 542000 -- (-849.891) (-846.082) [-841.131] (-853.267) * (-839.168) [-842.089] (-846.026) (-846.039) -- 0:01:56 543000 -- (-842.303) (-844.231) (-850.719) [-839.148] * (-845.551) (-840.804) (-845.233) [-844.535] -- 0:01:56 544000 -- (-842.202) (-846.816) [-841.044] (-845.852) * [-849.503] (-843.216) (-843.510) (-845.130) -- 0:01:56 545000 -- (-840.792) [-843.330] (-838.278) (-849.140) * [-837.257] (-847.002) (-844.164) (-849.645) -- 0:01:56 Average standard deviation of split frequencies: 0.006279 546000 -- (-840.308) [-836.413] (-847.127) (-842.109) * [-840.851] (-844.853) (-842.642) (-857.898) -- 0:01:55 547000 -- [-844.698] (-838.873) (-847.298) (-846.328) * (-843.431) (-850.432) [-846.135] (-847.147) -- 0:01:55 548000 -- [-841.842] (-848.269) (-848.207) (-847.575) * (-844.119) (-846.438) (-848.717) [-847.354] -- 0:01:55 549000 -- (-847.905) (-853.354) (-849.589) [-852.530] * (-851.832) (-847.334) (-865.839) [-837.715] -- 0:01:55 550000 -- (-845.664) (-843.675) (-840.765) [-839.954] * [-847.287] (-841.103) (-846.887) (-841.574) -- 0:01:54 Average standard deviation of split frequencies: 0.005603 551000 -- (-853.391) (-845.223) [-842.764] (-850.402) * (-836.836) [-843.068] (-848.974) (-841.758) -- 0:01:54 552000 -- (-843.360) (-844.692) [-838.302] (-855.393) * (-846.276) (-841.285) (-844.559) [-844.715] -- 0:01:54 553000 -- (-840.545) (-848.180) [-847.255] (-844.001) * (-838.406) (-851.423) [-836.562] (-842.174) -- 0:01:53 554000 -- (-851.552) (-852.197) [-838.707] (-843.055) * [-835.897] (-842.385) (-843.435) (-843.296) -- 0:01:53 555000 -- (-839.554) (-852.052) (-841.867) [-835.193] * [-843.836] (-839.736) (-846.711) (-849.784) -- 0:01:53 Average standard deviation of split frequencies: 0.005087 556000 -- (-842.701) (-855.740) (-841.217) [-844.158] * (-845.795) (-847.731) [-840.861] (-840.849) -- 0:01:53 557000 -- [-845.802] (-848.639) (-844.397) (-846.530) * (-849.894) [-845.028] (-844.673) (-852.302) -- 0:01:52 558000 -- (-842.080) [-846.064] (-848.977) (-842.783) * [-838.236] (-855.377) (-839.638) (-844.583) -- 0:01:52 559000 -- [-840.736] (-842.076) (-844.827) (-845.256) * (-842.213) (-849.008) (-841.563) [-851.860] -- 0:01:52 560000 -- (-840.939) (-840.131) (-838.933) [-845.050] * [-842.917] (-850.663) (-844.048) (-842.335) -- 0:01:52 Average standard deviation of split frequencies: 0.005886 561000 -- (-846.234) (-845.954) (-841.483) [-848.009] * (-850.064) [-843.382] (-842.170) (-837.148) -- 0:01:51 562000 -- (-845.013) (-843.112) (-844.316) [-843.438] * [-843.781] (-839.456) (-842.402) (-845.138) -- 0:01:51 563000 -- [-845.886] (-847.907) (-844.044) (-838.581) * (-852.456) (-836.593) [-841.260] (-842.771) -- 0:01:51 564000 -- [-842.498] (-845.960) (-847.229) (-839.623) * (-841.921) [-848.344] (-851.250) (-844.990) -- 0:01:51 565000 -- (-839.351) (-844.798) [-846.289] (-849.599) * [-842.514] (-847.769) (-851.441) (-844.539) -- 0:01:50 Average standard deviation of split frequencies: 0.006512 566000 -- (-843.082) (-847.516) [-847.792] (-844.683) * (-850.055) (-843.643) [-841.514] (-843.341) -- 0:01:50 567000 -- (-854.796) [-844.485] (-845.460) (-847.249) * (-858.011) (-844.765) [-845.024] (-845.228) -- 0:01:50 568000 -- (-841.865) [-838.795] (-841.566) (-845.958) * (-844.174) [-846.548] (-848.464) (-847.578) -- 0:01:50 569000 -- (-840.694) (-845.324) [-845.465] (-845.993) * (-840.151) (-843.121) (-850.473) [-841.001] -- 0:01:49 570000 -- [-846.940] (-846.913) (-844.005) (-846.132) * (-842.465) (-845.334) (-846.248) [-839.140] -- 0:01:49 Average standard deviation of split frequencies: 0.006233 571000 -- (-843.652) [-844.313] (-849.863) (-845.188) * (-844.113) (-847.386) [-844.653] (-840.649) -- 0:01:49 572000 -- [-840.158] (-840.773) (-846.392) (-850.245) * (-841.326) [-843.305] (-853.236) (-847.270) -- 0:01:49 573000 -- (-840.543) (-840.815) [-838.387] (-841.749) * (-846.986) (-840.855) [-847.998] (-843.059) -- 0:01:48 574000 -- [-842.626] (-850.943) (-846.739) (-848.184) * (-848.353) [-836.193] (-844.781) (-845.453) -- 0:01:48 575000 -- [-840.046] (-845.108) (-848.441) (-853.454) * [-843.622] (-840.491) (-849.135) (-848.679) -- 0:01:48 Average standard deviation of split frequencies: 0.005878 576000 -- (-844.487) [-840.512] (-849.988) (-847.253) * [-842.659] (-845.437) (-847.907) (-851.939) -- 0:01:48 577000 -- (-835.325) [-842.093] (-858.645) (-847.358) * (-845.978) (-843.986) [-838.521] (-840.866) -- 0:01:47 578000 -- (-839.082) (-846.765) (-837.517) [-840.846] * [-840.509] (-860.251) (-842.684) (-842.412) -- 0:01:47 579000 -- (-844.356) (-843.925) [-842.577] (-852.359) * [-843.304] (-843.701) (-841.354) (-852.537) -- 0:01:47 580000 -- (-841.835) (-844.109) (-841.069) [-846.586] * (-839.649) (-842.441) (-844.928) [-848.171] -- 0:01:47 Average standard deviation of split frequencies: 0.005535 581000 -- (-845.746) [-855.023] (-848.528) (-845.972) * (-837.728) (-848.957) (-842.295) [-850.521] -- 0:01:46 582000 -- [-852.168] (-840.742) (-841.164) (-840.800) * [-844.320] (-842.954) (-841.414) (-844.491) -- 0:01:46 583000 -- (-845.593) (-852.055) (-837.598) [-842.807] * (-840.844) (-841.929) [-844.926] (-840.751) -- 0:01:46 584000 -- (-843.700) (-838.210) [-844.870] (-853.281) * [-839.155] (-849.452) (-844.554) (-843.440) -- 0:01:46 585000 -- (-848.876) (-847.137) [-839.909] (-836.970) * (-844.167) (-851.582) [-840.626] (-841.649) -- 0:01:45 Average standard deviation of split frequencies: 0.004754 586000 -- [-840.937] (-841.305) (-847.650) (-842.436) * (-844.168) (-851.245) [-845.954] (-843.967) -- 0:01:45 587000 -- [-834.239] (-840.318) (-843.534) (-847.568) * (-846.366) (-853.048) (-850.351) [-843.688] -- 0:01:45 588000 -- (-853.623) (-847.626) (-854.673) [-836.838] * [-837.253] (-848.083) (-847.590) (-849.074) -- 0:01:45 589000 -- (-842.989) (-840.853) [-848.819] (-840.514) * [-846.756] (-848.756) (-842.681) (-846.494) -- 0:01:44 590000 -- (-851.234) (-837.605) [-845.003] (-851.132) * (-840.960) (-849.871) [-842.741] (-843.094) -- 0:01:44 Average standard deviation of split frequencies: 0.004571 591000 -- (-855.360) (-848.276) [-839.349] (-844.440) * (-849.631) (-847.441) [-845.774] (-843.147) -- 0:01:44 592000 -- (-843.353) [-839.657] (-841.780) (-844.055) * (-846.009) [-847.515] (-843.439) (-847.431) -- 0:01:44 593000 -- (-848.027) [-838.809] (-848.305) (-846.199) * (-841.949) (-843.732) [-846.391] (-842.470) -- 0:01:43 594000 -- (-851.611) (-842.195) (-841.378) [-841.145] * (-841.785) [-839.042] (-844.843) (-842.826) -- 0:01:43 595000 -- (-850.646) (-846.323) (-845.007) [-842.609] * (-844.219) (-853.299) [-843.188] (-842.032) -- 0:01:43 Average standard deviation of split frequencies: 0.004314 596000 -- (-852.364) [-843.555] (-846.300) (-843.514) * (-851.449) (-841.223) (-843.972) [-844.980] -- 0:01:43 597000 -- (-843.780) [-843.366] (-847.216) (-855.317) * (-848.965) (-845.538) (-851.826) [-845.421] -- 0:01:42 598000 -- (-847.042) (-843.437) (-841.935) [-844.539] * [-840.913] (-846.173) (-845.370) (-846.874) -- 0:01:42 599000 -- (-851.471) (-841.609) (-850.423) [-840.635] * (-844.480) (-843.014) (-846.005) [-842.546] -- 0:01:42 600000 -- (-852.043) [-837.442] (-846.575) (-854.758) * (-839.213) (-852.535) [-838.608] (-845.038) -- 0:01:42 Average standard deviation of split frequencies: 0.004923 601000 -- (-845.003) (-853.083) (-838.355) [-842.032] * [-845.675] (-843.026) (-840.263) (-850.223) -- 0:01:41 602000 -- (-846.375) (-842.222) [-848.654] (-840.364) * (-843.735) (-850.659) (-850.408) [-843.088] -- 0:01:41 603000 -- (-840.405) (-840.655) [-841.815] (-852.950) * (-842.769) (-844.103) [-846.859] (-844.889) -- 0:01:41 604000 -- [-842.001] (-842.471) (-846.797) (-847.270) * [-853.501] (-845.190) (-847.777) (-849.688) -- 0:01:40 605000 -- [-846.780] (-843.713) (-841.300) (-843.240) * [-848.632] (-844.775) (-842.921) (-850.425) -- 0:01:40 Average standard deviation of split frequencies: 0.005375 606000 -- (-856.074) (-843.016) [-849.645] (-846.668) * [-850.967] (-847.044) (-849.351) (-849.662) -- 0:01:40 607000 -- (-846.747) (-847.483) (-842.161) [-840.331] * [-851.669] (-853.841) (-846.741) (-851.796) -- 0:01:40 608000 -- (-847.335) [-845.878] (-845.575) (-842.500) * (-843.327) (-843.853) [-846.648] (-856.461) -- 0:01:39 609000 -- (-852.770) (-839.444) (-848.864) [-840.400] * (-846.891) (-848.440) (-851.501) [-842.914] -- 0:01:39 610000 -- (-846.295) [-841.393] (-842.769) (-845.636) * (-845.786) (-853.327) [-839.486] (-850.813) -- 0:01:39 Average standard deviation of split frequencies: 0.005404 611000 -- [-849.267] (-847.469) (-842.565) (-846.625) * [-841.759] (-848.281) (-846.172) (-837.725) -- 0:01:39 612000 -- (-854.129) (-843.851) (-853.100) [-841.348] * [-845.865] (-843.818) (-845.398) (-844.903) -- 0:01:38 613000 -- (-848.275) (-842.824) (-854.260) [-838.979] * (-844.436) [-844.110] (-843.325) (-841.694) -- 0:01:38 614000 -- (-841.237) (-853.848) (-839.064) [-843.086] * (-846.063) [-843.160] (-841.340) (-848.017) -- 0:01:38 615000 -- [-841.312] (-848.731) (-841.260) (-842.880) * (-854.196) [-840.938] (-850.810) (-846.881) -- 0:01:38 Average standard deviation of split frequencies: 0.005496 616000 -- (-848.021) [-840.709] (-841.677) (-842.480) * (-847.456) (-845.178) [-835.293] (-837.781) -- 0:01:37 617000 -- (-837.820) (-849.057) (-846.267) [-855.006] * (-840.305) (-851.351) (-838.649) [-838.760] -- 0:01:37 618000 -- (-842.212) (-841.965) [-841.220] (-838.660) * (-850.429) [-839.399] (-848.893) (-847.796) -- 0:01:37 619000 -- [-838.366] (-855.601) (-846.476) (-846.898) * (-847.609) (-842.830) (-850.772) [-846.494] -- 0:01:37 620000 -- [-847.457] (-851.222) (-853.622) (-838.024) * [-841.402] (-846.679) (-848.749) (-844.200) -- 0:01:36 Average standard deviation of split frequencies: 0.005317 621000 -- [-848.643] (-845.041) (-840.774) (-842.769) * (-851.995) (-834.560) (-843.681) [-846.656] -- 0:01:36 622000 -- (-842.191) [-848.939] (-842.295) (-841.621) * (-853.027) [-844.945] (-846.908) (-850.567) -- 0:01:36 623000 -- (-842.764) (-849.039) (-847.287) [-843.982] * [-845.304] (-842.602) (-848.529) (-848.107) -- 0:01:36 624000 -- (-844.222) [-844.285] (-848.296) (-844.350) * (-842.645) [-838.015] (-842.491) (-848.793) -- 0:01:35 625000 -- (-848.068) (-844.009) (-846.524) [-847.881] * [-846.085] (-839.848) (-841.147) (-850.533) -- 0:01:35 Average standard deviation of split frequencies: 0.005066 626000 -- (-839.498) (-846.769) (-842.109) [-838.185] * (-842.283) [-842.254] (-844.594) (-841.010) -- 0:01:34 627000 -- (-841.462) [-850.962] (-844.738) (-851.897) * (-850.304) (-847.747) [-841.198] (-849.075) -- 0:01:35 628000 -- (-845.012) (-840.833) [-836.954] (-841.496) * [-851.881] (-851.200) (-845.530) (-853.297) -- 0:01:34 629000 -- (-848.215) (-840.353) (-841.641) [-839.506] * (-845.334) [-840.342] (-839.272) (-841.926) -- 0:01:34 630000 -- [-850.905] (-848.695) (-838.268) (-844.439) * (-843.211) (-845.848) (-841.428) [-850.135] -- 0:01:34 Average standard deviation of split frequencies: 0.005164 631000 -- (-848.347) (-853.425) (-843.346) [-840.869] * [-842.809] (-839.716) (-838.261) (-843.918) -- 0:01:34 632000 -- (-853.712) [-848.307] (-845.943) (-843.705) * [-855.219] (-850.799) (-840.881) (-848.707) -- 0:01:33 633000 -- (-841.840) [-845.239] (-839.384) (-841.003) * (-845.709) (-843.772) (-842.930) [-842.723] -- 0:01:33 634000 -- [-844.388] (-843.812) (-854.628) (-854.722) * (-843.124) (-850.310) (-838.570) [-843.150] -- 0:01:33 635000 -- (-847.532) (-850.658) [-845.938] (-843.146) * [-844.892] (-848.442) (-839.559) (-847.524) -- 0:01:33 Average standard deviation of split frequencies: 0.004986 636000 -- [-845.341] (-849.439) (-846.415) (-844.259) * [-836.501] (-855.481) (-844.170) (-845.106) -- 0:01:32 637000 -- [-841.029] (-843.006) (-843.473) (-849.386) * (-851.565) (-837.849) (-851.918) [-843.315] -- 0:01:32 638000 -- [-839.403] (-839.070) (-849.168) (-844.406) * (-845.747) (-849.571) [-840.482] (-842.855) -- 0:01:32 639000 -- (-849.115) [-838.554] (-844.665) (-848.097) * (-842.311) [-840.304] (-845.552) (-854.039) -- 0:01:32 640000 -- [-839.797] (-842.520) (-851.356) (-843.173) * (-855.448) (-840.957) [-844.390] (-849.201) -- 0:01:31 Average standard deviation of split frequencies: 0.005552 641000 -- (-847.563) (-839.993) [-840.182] (-853.584) * (-843.486) (-843.523) [-844.263] (-845.829) -- 0:01:31 642000 -- [-851.147] (-853.901) (-851.478) (-841.744) * (-846.398) [-842.346] (-848.483) (-853.644) -- 0:01:31 643000 -- (-848.236) [-842.974] (-848.990) (-850.031) * (-846.276) (-839.646) (-851.318) [-841.569] -- 0:01:31 644000 -- (-847.337) [-842.240] (-847.257) (-845.980) * (-847.376) [-845.066] (-848.027) (-846.642) -- 0:01:30 645000 -- (-848.166) [-843.078] (-850.388) (-842.215) * [-841.574] (-850.286) (-849.726) (-850.407) -- 0:01:30 Average standard deviation of split frequencies: 0.005042 646000 -- (-843.475) (-838.597) (-854.856) [-839.026] * [-837.594] (-847.079) (-847.305) (-843.112) -- 0:01:30 647000 -- (-854.639) (-842.202) (-841.643) [-843.271] * (-845.564) (-838.157) (-848.024) [-841.246] -- 0:01:30 648000 -- [-842.658] (-846.534) (-848.497) (-841.815) * [-837.176] (-843.719) (-848.330) (-840.810) -- 0:01:29 649000 -- (-847.430) (-844.978) (-847.286) [-840.175] * (-846.288) (-842.327) (-843.508) [-842.520] -- 0:01:29 650000 -- (-841.356) (-840.835) (-845.667) [-839.294] * [-842.123] (-851.944) (-843.335) (-842.169) -- 0:01:29 Average standard deviation of split frequencies: 0.005203 651000 -- (-851.627) [-840.687] (-845.363) (-845.318) * (-849.013) (-839.140) [-840.281] (-855.658) -- 0:01:28 652000 -- (-845.392) (-846.224) (-843.073) [-843.100] * [-847.599] (-848.493) (-847.727) (-839.512) -- 0:01:28 653000 -- [-841.078] (-850.945) (-845.755) (-850.896) * [-839.915] (-844.325) (-840.387) (-844.395) -- 0:01:28 654000 -- (-843.553) (-848.278) (-850.511) [-836.184] * [-849.100] (-846.294) (-844.848) (-843.546) -- 0:01:28 655000 -- (-849.062) (-852.315) (-857.897) [-841.144] * [-846.972] (-849.343) (-845.994) (-840.737) -- 0:01:27 Average standard deviation of split frequencies: 0.005226 656000 -- (-850.651) [-840.045] (-847.118) (-838.609) * [-846.959] (-849.501) (-840.496) (-839.742) -- 0:01:27 657000 -- [-847.336] (-848.101) (-859.096) (-842.936) * [-839.775] (-849.798) (-840.770) (-841.450) -- 0:01:27 658000 -- (-836.877) (-845.534) [-839.190] (-840.792) * (-846.446) (-848.991) (-844.974) [-840.219] -- 0:01:27 659000 -- (-846.220) (-847.962) [-841.591] (-847.584) * (-843.530) (-846.040) [-840.188] (-855.274) -- 0:01:26 660000 -- (-851.732) [-841.464] (-845.742) (-847.153) * (-848.868) (-847.514) [-841.022] (-839.510) -- 0:01:26 Average standard deviation of split frequencies: 0.004541 661000 -- (-844.758) (-845.688) (-856.283) [-841.701] * [-838.021] (-842.033) (-850.471) (-843.169) -- 0:01:26 662000 -- (-847.653) [-846.036] (-852.717) (-842.813) * (-843.032) [-842.258] (-845.152) (-852.747) -- 0:01:26 663000 -- (-844.926) [-849.262] (-849.391) (-844.188) * (-845.180) (-846.380) [-839.666] (-850.945) -- 0:01:25 664000 -- [-837.186] (-841.648) (-843.496) (-849.376) * (-848.693) (-844.047) [-849.833] (-851.028) -- 0:01:25 665000 -- (-841.343) (-849.841) (-844.512) [-843.373] * [-838.302] (-847.817) (-842.879) (-843.872) -- 0:01:25 Average standard deviation of split frequencies: 0.005405 666000 -- (-841.278) [-845.463] (-844.242) (-851.603) * [-842.920] (-844.976) (-836.414) (-847.884) -- 0:01:25 667000 -- (-858.422) (-840.196) [-842.582] (-845.320) * (-848.357) (-844.488) [-848.889] (-849.242) -- 0:01:24 668000 -- (-843.906) (-845.945) [-852.863] (-843.327) * (-855.320) (-844.057) (-850.894) [-849.687] -- 0:01:24 669000 -- [-849.667] (-851.852) (-847.778) (-841.124) * (-844.245) (-851.145) [-848.160] (-849.598) -- 0:01:24 670000 -- (-847.217) (-848.987) (-847.492) [-843.964] * (-840.909) [-846.060] (-846.602) (-842.308) -- 0:01:24 Average standard deviation of split frequencies: 0.005559 671000 -- (-852.290) (-850.103) (-849.992) [-848.315] * [-841.216] (-840.055) (-844.409) (-846.901) -- 0:01:23 672000 -- (-844.802) (-844.872) [-843.451] (-847.651) * [-844.354] (-843.176) (-852.247) (-842.673) -- 0:01:23 673000 -- [-843.641] (-851.993) (-850.126) (-845.297) * [-846.281] (-842.144) (-839.386) (-849.749) -- 0:01:23 674000 -- (-839.984) (-847.966) [-843.587] (-847.733) * (-854.196) [-837.034] (-837.185) (-851.163) -- 0:01:23 675000 -- (-844.520) [-850.498] (-847.646) (-845.730) * (-850.781) [-843.722] (-846.556) (-845.633) -- 0:01:22 Average standard deviation of split frequencies: 0.006149 676000 -- (-844.939) (-846.995) (-847.078) [-850.855] * (-855.223) (-846.857) (-846.438) [-841.942] -- 0:01:22 677000 -- (-847.155) [-849.779] (-839.921) (-849.024) * (-847.283) (-844.097) [-843.239] (-854.377) -- 0:01:22 678000 -- (-845.268) (-839.391) [-843.549] (-853.433) * (-849.534) [-849.305] (-842.660) (-843.870) -- 0:01:22 679000 -- (-849.852) (-855.259) (-841.230) [-845.581] * (-850.246) (-854.116) [-847.426] (-851.629) -- 0:01:21 680000 -- (-854.174) (-842.953) [-841.219] (-848.304) * (-842.469) (-850.622) [-837.897] (-848.819) -- 0:01:21 Average standard deviation of split frequencies: 0.006107 681000 -- [-848.712] (-843.322) (-840.687) (-841.940) * [-843.367] (-851.042) (-849.162) (-852.162) -- 0:01:21 682000 -- [-843.917] (-849.931) (-845.198) (-846.550) * (-843.787) (-853.938) (-841.660) [-845.041] -- 0:01:21 683000 -- (-853.920) (-843.546) (-849.425) [-843.205] * (-844.154) [-839.095] (-846.332) (-845.275) -- 0:01:20 684000 -- (-852.940) [-843.625] (-842.936) (-841.208) * (-841.176) (-846.529) [-848.668] (-847.145) -- 0:01:20 685000 -- (-848.417) [-849.000] (-847.082) (-852.987) * (-838.902) [-838.022] (-847.121) (-847.219) -- 0:01:20 Average standard deviation of split frequencies: 0.006185 686000 -- (-842.693) (-846.993) [-843.170] (-855.614) * (-847.258) (-851.182) (-840.590) [-846.472] -- 0:01:20 687000 -- [-847.025] (-841.798) (-847.014) (-842.696) * (-845.098) (-855.394) [-841.105] (-851.736) -- 0:01:19 688000 -- (-851.029) (-840.489) (-841.702) [-849.519] * (-842.573) [-850.203] (-844.548) (-852.080) -- 0:01:19 689000 -- (-843.925) (-845.543) [-838.511] (-839.427) * (-847.788) (-850.204) (-842.258) [-840.880] -- 0:01:19 690000 -- (-846.391) (-847.562) (-846.711) [-840.283] * [-846.113] (-847.907) (-843.855) (-846.459) -- 0:01:19 Average standard deviation of split frequencies: 0.006391 691000 -- [-839.895] (-851.831) (-847.417) (-860.475) * [-841.503] (-846.868) (-843.496) (-840.069) -- 0:01:18 692000 -- (-839.743) [-841.509] (-843.613) (-839.870) * [-835.596] (-844.830) (-842.414) (-847.520) -- 0:01:18 693000 -- (-843.408) [-843.585] (-843.318) (-845.926) * (-846.675) (-845.175) (-842.186) [-842.486] -- 0:01:18 694000 -- (-851.909) [-836.514] (-843.233) (-847.829) * [-846.572] (-837.685) (-842.725) (-837.588) -- 0:01:18 695000 -- [-838.603] (-842.064) (-850.880) (-849.186) * [-841.794] (-846.986) (-845.221) (-841.775) -- 0:01:17 Average standard deviation of split frequencies: 0.006650 696000 -- [-849.713] (-843.365) (-846.243) (-853.525) * (-840.508) (-851.323) [-842.422] (-840.547) -- 0:01:17 697000 -- (-843.570) (-847.867) (-846.352) [-847.844] * (-847.320) [-840.950] (-844.005) (-842.704) -- 0:01:17 698000 -- (-842.015) (-839.774) [-842.154] (-847.008) * [-844.405] (-841.316) (-844.926) (-841.005) -- 0:01:17 699000 -- (-850.865) (-846.157) (-840.771) [-845.842] * [-842.439] (-846.358) (-851.434) (-843.654) -- 0:01:16 700000 -- [-846.667] (-848.672) (-860.835) (-843.188) * (-841.733) (-844.075) [-840.994] (-849.519) -- 0:01:16 Average standard deviation of split frequencies: 0.006667 701000 -- (-844.658) (-851.195) (-841.170) [-837.049] * (-843.814) (-847.330) [-847.549] (-845.219) -- 0:01:16 702000 -- (-850.584) (-852.652) (-855.924) [-842.288] * (-849.267) (-847.454) (-845.794) [-842.904] -- 0:01:15 703000 -- (-847.098) (-846.321) (-844.912) [-843.071] * [-844.434] (-838.176) (-842.915) (-843.698) -- 0:01:15 704000 -- (-845.448) [-843.813] (-843.786) (-846.212) * (-851.659) (-839.950) (-853.721) [-842.942] -- 0:01:15 705000 -- [-847.763] (-849.670) (-855.126) (-840.509) * [-842.904] (-838.785) (-841.263) (-851.556) -- 0:01:15 Average standard deviation of split frequencies: 0.006981 706000 -- (-848.877) [-840.456] (-841.696) (-843.200) * (-846.459) (-844.410) (-847.021) [-837.766] -- 0:01:14 707000 -- (-853.181) [-844.730] (-847.944) (-846.379) * (-844.152) (-842.320) [-850.678] (-843.356) -- 0:01:14 708000 -- [-843.958] (-846.422) (-841.715) (-847.377) * (-846.684) (-843.889) [-845.420] (-848.347) -- 0:01:14 709000 -- (-844.112) [-847.620] (-845.161) (-839.296) * (-849.427) (-851.470) [-843.662] (-846.680) -- 0:01:14 710000 -- (-844.616) [-843.957] (-848.081) (-848.199) * (-847.281) (-853.223) [-840.294] (-844.050) -- 0:01:13 Average standard deviation of split frequencies: 0.007116 711000 -- (-852.205) (-855.344) [-847.782] (-849.592) * (-838.820) (-847.817) [-842.525] (-842.045) -- 0:01:13 712000 -- (-850.698) (-851.423) (-839.829) [-842.666] * (-845.394) [-849.392] (-847.709) (-842.272) -- 0:01:13 713000 -- (-858.599) (-840.863) (-856.190) [-840.466] * [-840.593] (-845.941) (-845.801) (-844.380) -- 0:01:13 714000 -- (-852.735) (-842.306) (-848.240) [-847.723] * [-844.540] (-850.729) (-853.723) (-844.394) -- 0:01:12 715000 -- (-854.646) (-840.550) [-842.089] (-846.957) * (-845.887) (-850.609) (-851.305) [-843.958] -- 0:01:12 Average standard deviation of split frequencies: 0.006763 716000 -- [-840.746] (-841.934) (-856.428) (-844.192) * (-846.381) (-849.101) (-841.263) [-843.377] -- 0:01:12 717000 -- [-840.158] (-844.345) (-842.566) (-859.627) * (-841.966) (-838.461) [-844.473] (-836.746) -- 0:01:12 718000 -- (-847.881) (-852.048) (-843.011) [-837.413] * (-852.129) (-847.636) [-838.079] (-841.442) -- 0:01:11 719000 -- (-844.385) [-845.642] (-850.419) (-842.357) * (-845.431) (-849.174) (-841.867) [-840.166] -- 0:01:11 720000 -- (-847.794) (-845.896) [-836.706] (-855.440) * (-842.078) (-841.539) (-841.537) [-845.105] -- 0:01:11 Average standard deviation of split frequencies: 0.006660 721000 -- [-838.222] (-845.090) (-852.908) (-856.733) * (-849.881) [-843.129] (-842.091) (-849.151) -- 0:01:11 722000 -- (-845.876) (-841.029) [-838.101] (-844.740) * (-841.995) (-837.882) (-863.069) [-841.069] -- 0:01:10 723000 -- (-837.621) (-851.772) (-842.254) [-840.827] * (-853.327) [-845.947] (-849.175) (-850.188) -- 0:01:10 724000 -- (-859.127) (-848.278) [-845.169] (-845.399) * (-853.252) (-848.879) (-841.853) [-848.051] -- 0:01:10 725000 -- (-846.094) (-839.502) (-845.896) [-846.197] * (-847.194) [-850.576] (-852.364) (-847.629) -- 0:01:10 Average standard deviation of split frequencies: 0.007379 726000 -- (-851.133) (-844.266) (-851.718) [-845.564] * (-849.850) (-849.399) (-842.482) [-842.590] -- 0:01:09 727000 -- (-849.790) (-847.019) [-842.618] (-840.027) * (-847.222) (-850.434) [-840.953] (-845.186) -- 0:01:09 728000 -- [-846.455] (-851.836) (-843.913) (-837.947) * (-851.073) (-841.750) [-846.400] (-845.977) -- 0:01:09 729000 -- (-854.543) (-847.959) (-841.977) [-845.071] * [-844.290] (-847.909) (-843.470) (-846.318) -- 0:01:09 730000 -- (-847.632) (-839.801) [-836.756] (-844.215) * (-840.584) (-845.568) [-852.837] (-844.525) -- 0:01:08 Average standard deviation of split frequencies: 0.006804 731000 -- (-840.602) (-844.101) [-840.727] (-854.529) * (-846.531) [-847.882] (-845.526) (-841.585) -- 0:01:08 732000 -- (-836.631) [-849.408] (-843.319) (-856.831) * (-852.095) (-846.995) [-841.993] (-848.765) -- 0:01:08 733000 -- (-836.005) (-843.385) [-845.080] (-849.041) * (-845.300) [-845.995] (-851.952) (-840.580) -- 0:01:08 734000 -- (-846.406) (-839.115) (-843.143) [-839.164] * (-861.337) (-850.642) (-845.334) [-845.626] -- 0:01:07 735000 -- (-849.464) (-853.994) [-847.139] (-840.802) * (-853.786) (-848.321) (-843.685) [-844.166] -- 0:01:07 Average standard deviation of split frequencies: 0.006754 736000 -- (-846.425) (-855.371) [-846.275] (-845.892) * [-837.211] (-848.451) (-848.644) (-848.771) -- 0:01:07 737000 -- [-839.928] (-841.634) (-841.705) (-847.564) * (-844.723) (-845.346) [-842.469] (-855.336) -- 0:01:07 738000 -- (-843.640) (-851.510) (-839.685) [-836.577] * [-838.495] (-850.654) (-856.013) (-848.144) -- 0:01:06 739000 -- (-842.420) (-851.300) [-845.767] (-847.200) * (-854.844) [-838.286] (-848.350) (-855.067) -- 0:01:06 740000 -- [-839.610] (-851.831) (-850.074) (-840.636) * (-842.079) (-838.997) (-851.586) [-842.628] -- 0:01:06 Average standard deviation of split frequencies: 0.006365 741000 -- (-847.237) (-847.917) [-848.074] (-841.298) * (-848.381) (-843.318) [-846.391] (-844.245) -- 0:01:06 742000 -- (-846.878) (-845.335) [-847.233] (-845.696) * [-846.275] (-841.059) (-853.129) (-852.883) -- 0:01:05 743000 -- (-849.466) (-838.347) (-844.592) [-848.433] * (-850.723) [-843.531] (-846.100) (-848.494) -- 0:01:05 744000 -- (-845.164) [-839.581] (-839.959) (-840.759) * (-844.491) (-851.532) (-841.907) [-843.815] -- 0:01:05 745000 -- (-840.884) (-858.012) (-839.585) [-839.577] * (-842.593) (-841.493) (-846.825) [-842.037] -- 0:01:05 Average standard deviation of split frequencies: 0.007813 746000 -- (-843.599) (-843.901) [-842.982] (-841.321) * [-845.701] (-842.632) (-846.725) (-849.532) -- 0:01:04 747000 -- (-839.451) [-847.868] (-838.740) (-849.046) * [-846.330] (-840.011) (-851.050) (-844.760) -- 0:01:04 748000 -- (-844.827) (-848.263) (-839.801) [-840.602] * (-860.269) (-837.983) [-843.139] (-850.422) -- 0:01:04 749000 -- (-841.902) [-846.191] (-844.788) (-843.999) * (-841.207) [-845.937] (-845.792) (-843.132) -- 0:01:04 750000 -- (-841.537) (-844.132) (-838.733) [-848.000] * (-844.387) [-833.836] (-856.373) (-853.157) -- 0:01:03 Average standard deviation of split frequencies: 0.007650 751000 -- (-842.829) (-845.777) [-847.910] (-842.492) * (-846.044) [-841.059] (-849.089) (-845.400) -- 0:01:03 752000 -- (-852.123) (-846.007) (-842.502) [-839.989] * (-839.049) (-842.357) [-844.601] (-844.722) -- 0:01:02 753000 -- [-837.730] (-847.502) (-851.305) (-839.842) * (-852.909) [-844.577] (-849.241) (-844.051) -- 0:01:02 754000 -- (-843.561) (-846.291) [-842.972] (-846.834) * (-844.821) (-844.005) [-839.430] (-842.192) -- 0:01:02 755000 -- [-841.116] (-846.833) (-845.837) (-845.640) * [-844.734] (-852.787) (-844.535) (-842.358) -- 0:01:02 Average standard deviation of split frequencies: 0.007653 756000 -- [-839.382] (-843.017) (-842.122) (-847.277) * (-842.535) [-844.105] (-844.378) (-844.243) -- 0:01:01 757000 -- (-844.372) (-844.919) [-847.334] (-853.448) * [-841.392] (-840.958) (-841.356) (-841.262) -- 0:01:01 758000 -- (-847.111) [-840.949] (-839.609) (-846.715) * (-838.818) (-847.718) [-838.084] (-852.492) -- 0:01:01 759000 -- (-847.987) (-845.735) [-848.482] (-849.287) * (-848.858) (-839.148) (-850.323) [-839.211] -- 0:01:01 760000 -- (-845.567) (-842.680) (-843.747) [-836.336] * (-850.512) (-853.561) (-847.396) [-841.281] -- 0:01:00 Average standard deviation of split frequencies: 0.008338 761000 -- (-847.476) [-847.762] (-850.106) (-847.362) * (-845.978) (-846.979) [-851.889] (-852.663) -- 0:01:00 762000 -- (-845.668) (-849.109) (-841.133) [-842.587] * [-842.809] (-845.603) (-836.925) (-838.891) -- 0:01:00 763000 -- (-842.395) (-848.203) [-838.742] (-851.627) * [-846.087] (-853.088) (-853.662) (-847.836) -- 0:01:00 764000 -- [-842.309] (-840.437) (-846.672) (-851.406) * [-843.085] (-850.402) (-848.954) (-848.165) -- 0:00:59 765000 -- (-847.845) [-842.797] (-845.741) (-846.505) * [-841.050] (-845.684) (-846.786) (-842.345) -- 0:00:59 Average standard deviation of split frequencies: 0.007609 766000 -- (-841.032) (-850.716) [-842.931] (-843.098) * (-849.203) (-848.984) (-852.818) [-842.139] -- 0:00:59 767000 -- (-847.748) (-847.263) [-838.371] (-840.643) * (-851.620) [-841.132] (-839.729) (-847.956) -- 0:00:59 768000 -- (-843.376) [-839.717] (-845.405) (-842.914) * (-850.953) [-843.014] (-844.670) (-837.824) -- 0:00:58 769000 -- [-839.608] (-842.677) (-836.880) (-848.135) * [-840.134] (-844.821) (-843.144) (-846.224) -- 0:00:58 770000 -- (-848.045) (-842.504) (-845.273) [-840.954] * [-848.499] (-843.174) (-842.839) (-847.737) -- 0:00:58 Average standard deviation of split frequencies: 0.007618 771000 -- (-846.599) (-851.625) [-846.954] (-845.821) * (-838.928) [-854.445] (-852.575) (-846.377) -- 0:00:58 772000 -- (-843.793) (-844.023) [-842.871] (-847.971) * (-842.876) (-842.962) [-843.891] (-844.453) -- 0:00:57 773000 -- (-842.050) (-846.431) (-848.192) [-844.254] * (-843.378) (-849.420) (-840.062) [-850.985] -- 0:00:57 774000 -- (-847.130) [-845.696] (-844.786) (-840.719) * (-847.971) (-851.012) [-842.972] (-843.402) -- 0:00:57 775000 -- (-842.615) [-849.429] (-848.226) (-844.973) * [-845.132] (-844.261) (-840.884) (-839.108) -- 0:00:57 Average standard deviation of split frequencies: 0.008118 776000 -- (-844.299) (-845.244) (-842.818) [-845.636] * (-847.696) (-853.191) [-841.791] (-844.193) -- 0:00:56 777000 -- (-852.328) [-845.595] (-841.374) (-842.482) * [-836.940] (-854.444) (-841.167) (-845.935) -- 0:00:56 778000 -- (-854.029) (-842.151) (-845.979) [-836.759] * (-850.942) (-839.990) [-848.512] (-841.040) -- 0:00:56 779000 -- (-844.707) (-842.203) (-842.467) [-841.457] * (-840.010) (-840.608) [-846.330] (-843.456) -- 0:00:56 780000 -- (-845.221) [-844.495] (-844.585) (-850.719) * [-842.043] (-846.665) (-848.532) (-852.236) -- 0:00:55 Average standard deviation of split frequencies: 0.008015 781000 -- (-846.318) (-847.814) (-853.047) [-848.914] * (-845.163) (-851.834) [-845.672] (-847.766) -- 0:00:55 782000 -- (-844.889) (-837.219) [-848.484] (-850.332) * (-849.723) (-853.403) [-837.931] (-848.497) -- 0:00:55 783000 -- (-844.675) [-844.912] (-861.993) (-842.577) * (-847.258) (-846.063) [-840.208] (-851.697) -- 0:00:55 784000 -- (-839.952) [-840.156] (-846.184) (-840.582) * (-844.481) (-846.640) [-839.638] (-847.953) -- 0:00:54 785000 -- [-843.710] (-847.153) (-841.586) (-845.944) * [-841.095] (-845.558) (-840.954) (-848.427) -- 0:00:54 Average standard deviation of split frequencies: 0.008397 786000 -- (-843.683) (-843.916) [-841.595] (-844.655) * (-843.218) (-843.937) [-838.761] (-851.807) -- 0:00:54 787000 -- [-837.630] (-844.411) (-847.274) (-844.757) * (-850.267) (-844.150) [-844.186] (-847.097) -- 0:00:54 788000 -- [-839.453] (-842.821) (-851.365) (-843.423) * (-843.574) (-840.742) [-842.088] (-842.791) -- 0:00:53 789000 -- (-842.029) (-842.808) [-840.452] (-842.198) * (-845.437) (-848.714) [-843.291] (-843.684) -- 0:00:53 790000 -- [-848.031] (-845.842) (-847.597) (-839.052) * [-849.676] (-841.811) (-843.710) (-843.196) -- 0:00:53 Average standard deviation of split frequencies: 0.008184 791000 -- (-841.487) [-846.087] (-846.327) (-841.757) * (-848.635) (-850.368) (-843.390) [-842.515] -- 0:00:53 792000 -- (-844.531) [-845.029] (-847.076) (-843.782) * (-843.127) (-847.418) [-840.198] (-839.541) -- 0:00:53 793000 -- (-844.131) (-854.678) [-841.118] (-841.230) * [-840.527] (-845.704) (-843.455) (-848.725) -- 0:00:52 794000 -- [-838.952] (-855.382) (-842.974) (-843.166) * [-847.784] (-845.550) (-847.524) (-846.718) -- 0:00:52 795000 -- (-846.301) [-844.378] (-850.652) (-847.858) * (-848.104) [-846.625] (-846.921) (-849.503) -- 0:00:52 Average standard deviation of split frequencies: 0.008722 796000 -- (-847.214) [-842.667] (-848.111) (-861.699) * (-835.324) (-849.053) (-856.188) [-843.687] -- 0:00:52 797000 -- (-850.691) (-848.299) (-854.045) [-838.465] * (-855.994) (-841.893) [-844.382] (-843.370) -- 0:00:51 798000 -- (-848.984) [-841.609] (-843.645) (-845.174) * (-846.202) (-844.800) (-840.413) [-844.960] -- 0:00:51 799000 -- [-847.025] (-843.623) (-845.780) (-844.924) * [-838.195] (-843.233) (-847.459) (-850.007) -- 0:00:51 800000 -- (-847.325) (-840.850) (-851.341) [-851.556] * (-846.871) [-840.812] (-846.720) (-853.877) -- 0:00:50 Average standard deviation of split frequencies: 0.009046 801000 -- (-841.515) (-850.042) (-846.137) [-842.700] * (-841.184) (-850.705) [-841.023] (-860.802) -- 0:00:50 802000 -- (-847.776) (-844.053) [-844.327] (-847.085) * (-845.879) (-843.585) (-847.493) [-841.057] -- 0:00:50 803000 -- (-843.476) [-843.166] (-844.131) (-852.977) * (-841.370) (-840.228) [-845.519] (-847.642) -- 0:00:50 804000 -- (-842.327) (-843.661) (-840.356) [-839.732] * (-850.950) (-840.115) [-844.442] (-842.306) -- 0:00:49 805000 -- (-840.541) (-850.041) (-843.926) [-841.489] * (-842.807) (-847.036) (-840.762) [-843.690] -- 0:00:49 Average standard deviation of split frequencies: 0.009039 806000 -- (-841.531) (-848.752) [-842.452] (-846.285) * (-839.609) [-849.970] (-846.649) (-841.205) -- 0:00:49 807000 -- (-848.000) [-839.877] (-844.571) (-843.449) * (-842.639) [-842.819] (-851.429) (-852.410) -- 0:00:49 808000 -- (-845.328) [-838.850] (-847.263) (-840.456) * (-849.257) (-844.486) (-846.442) [-843.699] -- 0:00:48 809000 -- (-850.566) (-837.957) (-838.713) [-842.163] * (-848.954) (-845.626) [-838.960] (-846.520) -- 0:00:48 810000 -- (-848.025) (-843.556) [-838.505] (-843.907) * (-847.461) (-841.127) [-843.626] (-851.388) -- 0:00:48 Average standard deviation of split frequencies: 0.008987 811000 -- (-840.563) (-848.016) [-840.882] (-854.455) * (-846.130) [-843.670] (-850.125) (-841.621) -- 0:00:48 812000 -- [-842.197] (-848.248) (-846.305) (-841.407) * [-851.309] (-841.748) (-844.434) (-840.193) -- 0:00:47 813000 -- [-839.809] (-854.785) (-852.126) (-849.068) * (-842.178) [-844.517] (-848.051) (-850.945) -- 0:00:47 814000 -- (-848.309) (-847.949) (-846.150) [-841.419] * (-841.309) [-839.933] (-849.938) (-843.251) -- 0:00:47 815000 -- [-845.457] (-840.662) (-844.266) (-851.729) * (-842.114) (-842.116) (-844.126) [-852.936] -- 0:00:46 Average standard deviation of split frequencies: 0.008560 816000 -- [-837.165] (-846.331) (-849.811) (-840.423) * (-848.498) [-846.706] (-848.190) (-844.712) -- 0:00:46 817000 -- (-849.194) (-848.717) [-843.846] (-846.392) * (-838.283) [-841.005] (-843.123) (-855.312) -- 0:00:46 818000 -- [-846.410] (-851.118) (-845.669) (-844.147) * (-846.284) (-844.176) (-842.083) [-842.745] -- 0:00:46 819000 -- (-837.747) (-847.022) [-847.743] (-854.026) * (-841.803) (-852.532) (-848.194) [-841.858] -- 0:00:45 820000 -- (-843.720) (-847.600) [-840.126] (-853.544) * (-843.397) (-865.609) [-848.103] (-846.318) -- 0:00:45 Average standard deviation of split frequencies: 0.007990 821000 -- (-849.061) (-844.827) (-841.594) [-843.453] * (-838.503) (-845.844) [-847.742] (-853.498) -- 0:00:45 822000 -- [-849.046] (-842.843) (-843.559) (-847.624) * (-842.818) (-851.546) (-849.186) [-843.879] -- 0:00:45 823000 -- [-835.206] (-843.082) (-858.942) (-845.127) * (-861.590) (-846.526) [-840.416] (-838.668) -- 0:00:44 824000 -- (-843.151) (-844.280) [-842.232] (-843.327) * (-841.334) (-845.331) (-843.814) [-842.397] -- 0:00:44 825000 -- (-847.658) [-840.231] (-844.165) (-844.868) * (-844.157) (-844.711) [-838.813] (-845.214) -- 0:00:44 Average standard deviation of split frequencies: 0.007938 826000 -- (-847.875) [-838.298] (-848.878) (-842.723) * (-851.076) (-853.689) [-838.295] (-850.136) -- 0:00:44 827000 -- [-847.897] (-845.528) (-849.210) (-843.758) * (-844.833) [-838.575] (-844.112) (-851.619) -- 0:00:43 828000 -- (-840.536) (-840.731) (-842.762) [-842.370] * (-848.742) (-846.121) [-844.113] (-839.582) -- 0:00:43 829000 -- [-840.482] (-844.754) (-842.224) (-845.404) * [-850.043] (-840.176) (-840.768) (-852.242) -- 0:00:43 830000 -- (-846.331) [-844.273] (-839.158) (-839.294) * (-851.851) [-843.968] (-846.553) (-850.934) -- 0:00:43 Average standard deviation of split frequencies: 0.007945 831000 -- (-842.206) [-843.052] (-843.503) (-846.877) * (-849.388) (-842.187) (-853.644) [-853.001] -- 0:00:42 832000 -- [-850.177] (-848.079) (-847.400) (-848.268) * (-844.067) (-848.576) (-846.327) [-843.990] -- 0:00:42 833000 -- (-843.649) (-846.944) (-851.702) [-853.116] * (-842.766) (-842.192) [-841.218] (-850.669) -- 0:00:42 834000 -- (-842.867) (-849.029) [-844.430] (-848.900) * (-846.483) [-840.725] (-843.967) (-844.432) -- 0:00:42 835000 -- [-840.478] (-857.162) (-840.928) (-851.418) * (-847.104) (-851.580) (-845.788) [-841.620] -- 0:00:41 Average standard deviation of split frequencies: 0.007638 836000 -- (-849.069) (-845.619) [-840.217] (-857.281) * [-837.161] (-847.288) (-842.954) (-849.104) -- 0:00:41 837000 -- (-848.022) (-850.461) [-847.357] (-848.646) * (-847.189) (-849.274) (-841.392) [-844.547] -- 0:00:41 838000 -- (-849.533) (-849.228) (-838.880) [-841.762] * (-836.781) [-841.124] (-846.421) (-856.774) -- 0:00:41 839000 -- (-844.122) (-847.065) (-839.421) [-843.631] * [-853.930] (-841.527) (-844.079) (-847.654) -- 0:00:40 840000 -- (-844.658) [-837.446] (-846.162) (-843.061) * [-838.807] (-850.937) (-847.447) (-838.058) -- 0:00:40 Average standard deviation of split frequencies: 0.007647 841000 -- (-840.760) [-844.818] (-849.961) (-838.581) * (-845.140) (-846.871) [-842.482] (-843.523) -- 0:00:40 842000 -- (-849.596) (-847.282) (-849.805) [-842.414] * [-842.414] (-852.771) (-844.541) (-851.035) -- 0:00:40 843000 -- (-852.579) (-858.172) (-854.489) [-844.417] * (-855.097) [-846.461] (-842.598) (-853.810) -- 0:00:39 844000 -- (-847.723) [-846.265] (-859.669) (-845.841) * (-843.335) [-840.453] (-850.042) (-845.247) -- 0:00:39 845000 -- (-848.466) (-840.309) (-845.138) [-841.727] * (-839.779) [-843.681] (-847.632) (-843.726) -- 0:00:39 Average standard deviation of split frequencies: 0.007598 846000 -- [-843.341] (-853.114) (-847.192) (-847.493) * (-854.556) (-844.720) [-840.459] (-849.152) -- 0:00:39 847000 -- [-844.563] (-843.589) (-838.954) (-853.192) * (-846.713) (-849.601) (-847.252) [-848.213] -- 0:00:38 848000 -- [-841.908] (-845.599) (-866.613) (-852.812) * (-842.648) (-835.914) [-842.823] (-853.046) -- 0:00:38 849000 -- (-846.239) (-838.069) (-842.446) [-844.883] * (-848.218) [-840.534] (-844.019) (-843.659) -- 0:00:38 850000 -- (-863.111) [-834.631] (-843.265) (-838.829) * (-844.026) (-846.058) [-843.416] (-840.908) -- 0:00:38 Average standard deviation of split frequencies: 0.007305 851000 -- (-848.568) (-849.489) (-842.490) [-847.562] * (-841.933) (-841.284) (-848.573) [-841.043] -- 0:00:37 852000 -- (-841.725) [-852.181] (-846.605) (-845.092) * (-846.578) (-846.830) [-839.784] (-845.047) -- 0:00:37 853000 -- [-838.877] (-842.448) (-854.683) (-848.122) * (-843.605) (-850.036) [-839.151] (-839.058) -- 0:00:37 854000 -- (-848.852) [-843.480] (-840.640) (-845.656) * (-846.542) (-845.902) (-843.268) [-837.990] -- 0:00:37 855000 -- (-841.638) (-843.853) (-843.119) [-839.143] * [-845.930] (-841.809) (-843.506) (-848.045) -- 0:00:36 Average standard deviation of split frequencies: 0.007209 856000 -- (-852.151) [-844.760] (-844.419) (-848.652) * (-848.430) (-847.825) (-846.885) [-844.603] -- 0:00:36 857000 -- (-851.686) [-837.211] (-844.864) (-848.096) * (-841.582) (-851.561) [-842.828] (-846.443) -- 0:00:36 858000 -- (-854.385) (-841.273) (-848.158) [-840.290] * (-848.793) (-845.205) (-841.847) [-838.169] -- 0:00:36 859000 -- (-847.590) [-837.943] (-850.576) (-855.549) * (-848.657) (-838.491) [-843.163] (-850.969) -- 0:00:35 860000 -- [-840.180] (-834.899) (-842.308) (-848.548) * (-842.476) (-844.646) [-840.068] (-847.764) -- 0:00:35 Average standard deviation of split frequencies: 0.006921 861000 -- (-850.984) [-844.439] (-844.606) (-847.959) * [-845.201] (-848.578) (-854.921) (-844.962) -- 0:00:35 862000 -- [-849.886] (-847.358) (-842.409) (-847.944) * (-854.063) (-843.907) (-842.016) [-846.432] -- 0:00:35 863000 -- (-847.699) (-849.851) (-852.331) [-846.841] * [-852.444] (-846.589) (-842.598) (-842.473) -- 0:00:34 864000 -- (-844.499) (-839.059) [-840.725] (-844.526) * (-843.295) (-842.275) (-838.714) [-844.692] -- 0:00:34 865000 -- (-853.389) (-844.727) (-844.870) [-842.649] * (-847.509) [-836.426] (-846.388) (-842.725) -- 0:00:34 Average standard deviation of split frequencies: 0.006879 866000 -- (-845.356) [-841.494] (-841.885) (-843.272) * (-842.994) (-843.938) (-846.079) [-844.320] -- 0:00:34 867000 -- (-847.149) (-852.181) [-842.237] (-848.749) * (-842.883) (-847.864) (-846.849) [-848.799] -- 0:00:33 868000 -- [-838.034] (-854.946) (-854.214) (-844.559) * (-846.249) (-845.892) (-842.501) [-840.345] -- 0:00:33 869000 -- (-848.336) (-860.612) [-846.788] (-844.875) * (-848.447) [-846.246] (-851.748) (-842.785) -- 0:00:33 870000 -- (-839.987) (-852.869) (-839.083) [-846.845] * (-848.452) (-852.726) (-845.710) [-842.432] -- 0:00:33 Average standard deviation of split frequencies: 0.007039 871000 -- (-849.620) (-847.686) [-843.224] (-843.492) * (-850.953) (-838.888) [-843.380] (-852.671) -- 0:00:32 872000 -- (-842.903) (-858.201) (-839.854) [-852.986] * (-849.089) (-852.436) (-850.939) [-849.474] -- 0:00:32 873000 -- (-845.792) (-846.370) (-842.752) [-845.402] * (-851.918) (-843.362) [-845.174] (-846.438) -- 0:00:32 874000 -- (-850.310) (-842.018) (-851.564) [-841.097] * (-865.093) (-841.693) (-843.808) [-850.095] -- 0:00:32 875000 -- [-845.039] (-841.416) (-851.976) (-847.825) * (-847.207) (-840.959) (-852.990) [-847.836] -- 0:00:31 Average standard deviation of split frequencies: 0.007045 876000 -- (-844.516) [-840.447] (-848.318) (-849.269) * (-843.495) (-850.349) (-858.919) [-849.646] -- 0:00:31 877000 -- [-842.438] (-839.966) (-843.635) (-849.591) * [-838.979] (-838.890) (-845.053) (-850.162) -- 0:00:31 878000 -- [-840.891] (-848.513) (-843.337) (-849.587) * (-849.752) [-841.692] (-839.503) (-842.905) -- 0:00:30 879000 -- (-846.550) [-838.743] (-852.591) (-846.621) * (-848.155) (-846.369) [-839.371] (-849.194) -- 0:00:30 880000 -- (-839.497) (-847.979) (-840.051) [-843.929] * (-844.790) [-843.842] (-844.245) (-848.369) -- 0:00:30 Average standard deviation of split frequencies: 0.006861 881000 -- (-854.462) (-837.982) [-840.216] (-846.423) * [-841.547] (-845.126) (-852.544) (-842.963) -- 0:00:30 882000 -- (-847.967) (-851.594) [-842.145] (-843.921) * (-846.777) (-839.604) (-855.913) [-848.779] -- 0:00:29 883000 -- (-842.691) [-841.174] (-840.637) (-841.553) * (-841.993) [-839.000] (-846.468) (-851.362) -- 0:00:29 884000 -- (-848.701) (-844.992) (-842.573) [-846.360] * (-841.149) (-842.263) [-847.532] (-851.439) -- 0:00:29 885000 -- (-850.632) [-844.104] (-845.753) (-843.552) * (-847.769) (-840.610) [-843.568] (-840.852) -- 0:00:29 Average standard deviation of split frequencies: 0.006820 886000 -- (-843.440) [-843.948] (-842.954) (-844.977) * (-843.191) [-845.124] (-846.196) (-847.333) -- 0:00:28 887000 -- (-843.852) (-841.400) (-861.376) [-845.598] * (-853.118) (-842.440) [-843.643] (-848.994) -- 0:00:28 888000 -- (-841.683) (-853.589) [-841.411] (-851.526) * (-844.577) (-847.028) (-842.913) [-839.442] -- 0:00:28 889000 -- (-849.498) (-849.001) [-843.973] (-839.133) * (-843.579) [-840.212] (-845.461) (-846.022) -- 0:00:28 890000 -- (-849.502) (-846.166) [-841.905] (-849.806) * (-844.131) [-846.363] (-850.826) (-838.112) -- 0:00:27 Average standard deviation of split frequencies: 0.006544 891000 -- (-843.763) (-848.723) [-840.103] (-842.440) * (-847.493) (-845.105) [-842.519] (-836.487) -- 0:00:27 892000 -- (-850.017) (-841.687) [-848.870] (-845.080) * (-844.477) (-849.695) [-838.651] (-850.549) -- 0:00:27 893000 -- (-847.438) [-842.600] (-845.015) (-848.444) * [-838.442] (-847.818) (-845.441) (-841.502) -- 0:00:27 894000 -- (-839.421) (-849.580) [-837.910] (-840.502) * (-846.798) (-849.331) (-838.269) [-840.233] -- 0:00:26 895000 -- (-853.145) (-846.640) (-851.109) [-836.653] * (-839.291) (-841.740) [-842.416] (-850.497) -- 0:00:26 Average standard deviation of split frequencies: 0.006744 896000 -- (-850.175) (-841.666) (-849.428) [-841.421] * (-838.371) (-845.438) [-838.976] (-840.569) -- 0:00:26 897000 -- [-843.586] (-843.466) (-843.433) (-848.143) * (-848.949) (-840.751) [-838.731] (-840.234) -- 0:00:26 898000 -- (-842.080) [-836.143] (-845.783) (-844.555) * (-851.033) [-840.518] (-850.753) (-845.388) -- 0:00:25 899000 -- (-845.573) (-853.475) (-853.034) [-841.969] * (-845.713) (-850.525) (-841.581) [-844.371] -- 0:00:25 900000 -- [-848.177] (-846.713) (-848.729) (-843.184) * (-844.005) (-846.160) (-845.265) [-841.201] -- 0:00:25 Average standard deviation of split frequencies: 0.006614 901000 -- (-843.880) (-845.636) [-845.061] (-844.188) * (-847.382) [-840.870] (-844.860) (-843.546) -- 0:00:25 902000 -- (-838.785) (-844.604) (-844.655) [-843.441] * (-849.041) [-845.960] (-839.696) (-846.604) -- 0:00:24 903000 -- (-843.042) (-836.681) (-844.808) [-849.265] * [-845.797] (-845.506) (-851.229) (-845.095) -- 0:00:24 904000 -- (-839.472) (-856.041) (-844.089) [-841.344] * (-836.149) (-844.662) (-849.376) [-844.191] -- 0:00:24 905000 -- (-846.541) [-838.067] (-849.656) (-846.043) * [-836.964] (-843.605) (-842.149) (-840.996) -- 0:00:24 Average standard deviation of split frequencies: 0.007190 906000 -- (-846.039) [-845.686] (-849.271) (-839.476) * [-837.688] (-841.199) (-852.618) (-851.537) -- 0:00:23 907000 -- (-837.674) [-848.714] (-850.657) (-852.015) * (-847.307) [-847.607] (-858.378) (-841.666) -- 0:00:23 908000 -- (-840.184) [-847.625] (-850.171) (-843.374) * (-846.454) (-847.477) (-850.520) [-844.112] -- 0:00:23 909000 -- [-840.615] (-847.440) (-849.837) (-840.825) * [-837.254] (-842.311) (-852.325) (-843.715) -- 0:00:23 910000 -- (-843.993) (-843.805) [-846.662] (-843.093) * (-842.039) (-844.178) (-844.104) [-849.364] -- 0:00:22 Average standard deviation of split frequencies: 0.006965 911000 -- (-851.361) (-851.337) [-847.798] (-850.455) * [-846.743] (-842.574) (-841.869) (-841.053) -- 0:00:22 912000 -- (-846.107) (-850.927) [-850.012] (-841.555) * (-847.718) (-851.422) (-848.348) [-837.108] -- 0:00:22 913000 -- [-844.181] (-852.135) (-859.534) (-846.532) * (-847.251) [-849.346] (-844.332) (-844.733) -- 0:00:22 914000 -- [-842.404] (-843.840) (-847.707) (-845.572) * (-845.094) [-839.870] (-840.585) (-846.755) -- 0:00:21 915000 -- (-842.108) [-844.720] (-853.075) (-856.391) * (-839.082) (-849.525) [-843.163] (-853.320) -- 0:00:21 Average standard deviation of split frequencies: 0.007018 916000 -- (-845.202) [-850.022] (-842.800) (-846.074) * (-844.836) (-845.953) [-840.629] (-842.603) -- 0:00:21 917000 -- [-843.225] (-849.757) (-849.828) (-845.735) * (-845.240) (-849.776) [-842.080] (-845.491) -- 0:00:21 918000 -- (-852.895) (-852.298) [-841.409] (-848.892) * (-856.891) [-850.292] (-851.037) (-847.177) -- 0:00:20 919000 -- (-846.785) (-841.227) [-842.007] (-842.272) * (-846.937) [-842.712] (-840.511) (-847.306) -- 0:00:20 920000 -- (-844.236) (-844.239) [-844.374] (-853.153) * (-837.340) (-846.624) (-841.698) [-845.757] -- 0:00:20 Average standard deviation of split frequencies: 0.006843 921000 -- (-859.354) (-849.133) (-845.932) [-849.107] * (-841.085) [-845.340] (-848.220) (-842.290) -- 0:00:20 922000 -- (-850.421) (-841.375) (-840.450) [-838.389] * (-844.123) (-843.849) [-842.717] (-842.717) -- 0:00:19 923000 -- (-846.894) (-841.927) [-844.257] (-845.712) * [-844.639] (-846.062) (-840.027) (-853.765) -- 0:00:19 924000 -- (-850.300) (-840.142) (-842.451) [-845.207] * (-840.207) [-840.394] (-848.716) (-848.450) -- 0:00:19 925000 -- (-850.442) [-837.707] (-845.102) (-844.927) * (-844.270) (-851.571) [-841.137] (-855.554) -- 0:00:19 Average standard deviation of split frequencies: 0.006988 926000 -- (-840.844) [-851.333] (-844.759) (-847.601) * (-849.320) (-845.520) [-837.348] (-846.038) -- 0:00:18 927000 -- (-843.274) [-846.491] (-847.998) (-843.369) * [-842.211] (-854.012) (-847.829) (-840.275) -- 0:00:18 928000 -- [-844.956] (-847.392) (-847.697) (-843.348) * [-841.777] (-851.266) (-844.590) (-853.065) -- 0:00:18 929000 -- (-845.607) (-852.254) (-851.636) [-841.274] * (-845.121) [-844.517] (-842.437) (-849.583) -- 0:00:18 930000 -- (-847.842) [-846.823] (-847.316) (-843.417) * (-850.670) (-849.258) (-846.268) [-847.488] -- 0:00:17 Average standard deviation of split frequencies: 0.006631 931000 -- (-850.840) (-845.073) (-847.731) [-850.911] * [-841.634] (-839.296) (-853.915) (-847.810) -- 0:00:17 932000 -- [-843.041] (-861.023) (-843.756) (-839.384) * (-855.020) (-853.047) [-843.303] (-855.380) -- 0:00:17 933000 -- [-836.689] (-848.958) (-844.836) (-846.800) * (-850.427) [-845.384] (-837.946) (-859.524) -- 0:00:17 934000 -- (-844.075) (-842.498) (-843.596) [-846.362] * (-857.662) (-840.905) [-847.905] (-854.389) -- 0:00:16 935000 -- (-851.824) (-847.159) [-845.964] (-839.190) * (-840.996) [-847.382] (-859.981) (-843.497) -- 0:00:16 Average standard deviation of split frequencies: 0.006822 936000 -- (-844.987) [-841.669] (-855.037) (-845.061) * (-841.090) (-846.423) [-840.249] (-841.259) -- 0:00:16 937000 -- (-852.821) [-839.192] (-847.513) (-843.802) * (-839.504) [-838.524] (-840.579) (-856.953) -- 0:00:16 938000 -- (-844.810) (-844.901) [-844.113] (-848.843) * (-838.182) (-847.862) [-842.489] (-847.123) -- 0:00:15 939000 -- (-847.189) (-840.553) [-840.704] (-847.849) * (-845.575) (-861.967) (-847.531) [-842.306] -- 0:00:15 940000 -- (-847.655) (-848.652) [-850.386] (-844.751) * (-848.568) (-845.497) (-840.158) [-840.659] -- 0:00:15 Average standard deviation of split frequencies: 0.006743 941000 -- (-856.213) (-846.696) [-841.684] (-849.911) * [-840.997] (-842.257) (-853.401) (-848.091) -- 0:00:14 942000 -- (-848.796) [-848.094] (-846.738) (-846.415) * (-853.410) (-838.762) [-844.670] (-852.220) -- 0:00:14 943000 -- (-844.294) (-842.601) (-850.620) [-843.037] * (-847.879) [-848.526] (-847.912) (-847.419) -- 0:00:14 944000 -- (-851.129) [-837.385] (-847.609) (-837.418) * (-845.118) (-848.122) [-842.526] (-845.535) -- 0:00:14 945000 -- (-840.106) (-855.293) (-847.311) [-850.743] * (-851.496) [-839.910] (-854.353) (-837.158) -- 0:00:13 Average standard deviation of split frequencies: 0.006705 946000 -- (-847.743) (-845.161) [-837.946] (-842.999) * (-850.586) (-843.098) [-845.414] (-841.699) -- 0:00:13 947000 -- (-856.868) (-849.415) [-842.373] (-841.300) * [-842.118] (-846.420) (-843.378) (-839.322) -- 0:00:13 948000 -- [-849.581] (-844.819) (-845.720) (-842.552) * (-849.914) (-857.741) [-836.764] (-845.082) -- 0:00:13 949000 -- (-850.685) (-853.802) (-850.791) [-837.813] * [-844.210] (-846.464) (-846.457) (-841.971) -- 0:00:12 950000 -- (-851.944) (-840.868) (-843.947) [-841.977] * [-844.319] (-838.046) (-839.196) (-856.822) -- 0:00:12 Average standard deviation of split frequencies: 0.007032 951000 -- (-852.331) (-840.061) [-846.605] (-856.166) * (-846.365) (-848.162) [-839.037] (-844.639) -- 0:00:12 952000 -- (-848.003) [-850.182] (-847.830) (-857.057) * (-854.930) (-846.071) [-845.034] (-857.947) -- 0:00:12 953000 -- (-844.868) (-839.845) (-847.540) [-848.251] * (-844.281) (-851.169) [-846.532] (-841.916) -- 0:00:11 954000 -- (-838.048) (-844.613) (-847.283) [-839.809] * [-835.695] (-847.092) (-845.153) (-845.925) -- 0:00:11 955000 -- (-843.911) (-849.081) [-844.260] (-842.271) * [-840.404] (-851.127) (-841.297) (-848.774) -- 0:00:11 Average standard deviation of split frequencies: 0.006903 956000 -- [-850.186] (-838.102) (-861.788) (-844.872) * (-848.238) (-849.377) (-844.692) [-837.955] -- 0:00:11 957000 -- [-850.876] (-844.735) (-850.178) (-843.856) * (-849.258) [-844.849] (-844.689) (-851.430) -- 0:00:10 958000 -- (-848.783) (-840.174) (-842.560) [-841.197] * (-850.983) [-848.664] (-842.118) (-845.993) -- 0:00:10 959000 -- (-857.395) (-851.368) (-845.414) [-842.608] * [-841.548] (-850.711) (-846.430) (-843.059) -- 0:00:10 960000 -- (-846.268) (-854.569) [-841.350] (-842.676) * (-847.661) [-841.042] (-844.566) (-845.525) -- 0:00:10 Average standard deviation of split frequencies: 0.006245 961000 -- [-842.977] (-846.573) (-849.565) (-845.336) * (-854.740) (-843.105) [-842.663] (-843.527) -- 0:00:09 962000 -- [-839.776] (-840.677) (-847.316) (-848.283) * (-840.617) (-840.916) [-843.172] (-843.552) -- 0:00:09 963000 -- (-847.772) [-841.302] (-839.227) (-845.874) * (-844.041) (-842.952) [-846.161] (-840.554) -- 0:00:09 964000 -- (-842.572) [-844.829] (-849.393) (-858.061) * [-838.498] (-840.353) (-838.198) (-851.616) -- 0:00:09 965000 -- (-842.143) (-848.944) [-839.010] (-841.777) * (-841.418) (-842.827) [-840.354] (-849.282) -- 0:00:08 Average standard deviation of split frequencies: 0.006610 966000 -- [-840.929] (-849.947) (-844.494) (-844.715) * (-844.383) (-845.522) (-843.223) [-848.854] -- 0:00:08 967000 -- [-849.751] (-839.416) (-846.874) (-844.700) * (-842.340) (-840.625) [-841.793] (-850.241) -- 0:00:08 968000 -- (-856.822) (-840.313) (-838.326) [-840.093] * (-855.736) [-843.192] (-838.926) (-845.459) -- 0:00:08 969000 -- (-849.296) (-848.644) [-840.350] (-838.260) * (-853.875) (-859.125) [-843.732] (-838.445) -- 0:00:07 970000 -- (-837.774) [-842.349] (-843.149) (-841.497) * [-839.601] (-846.933) (-849.495) (-845.153) -- 0:00:07 Average standard deviation of split frequencies: 0.006269 971000 -- (-850.829) (-847.104) [-843.664] (-847.381) * (-844.911) [-847.820] (-838.781) (-844.780) -- 0:00:07 972000 -- (-850.723) (-847.262) (-848.757) [-841.102] * (-841.980) (-846.113) (-836.867) [-837.676] -- 0:00:07 973000 -- [-844.115] (-844.847) (-842.071) (-846.707) * [-840.247] (-842.040) (-850.041) (-850.738) -- 0:00:06 974000 -- [-838.684] (-844.792) (-847.198) (-844.161) * (-843.206) (-840.603) (-846.699) [-838.707] -- 0:00:06 975000 -- (-841.517) (-850.310) (-849.607) [-840.781] * (-848.096) [-840.544] (-849.881) (-852.280) -- 0:00:06 Average standard deviation of split frequencies: 0.006586 976000 -- [-847.468] (-846.828) (-852.357) (-841.241) * [-842.082] (-851.079) (-842.442) (-843.850) -- 0:00:06 977000 -- [-848.061] (-849.681) (-844.581) (-845.166) * (-844.011) [-845.613] (-841.162) (-845.701) -- 0:00:05 978000 -- (-848.637) [-846.348] (-842.931) (-847.474) * (-840.199) [-837.984] (-838.352) (-841.219) -- 0:00:05 979000 -- (-839.249) (-847.703) [-847.040] (-842.910) * (-846.366) (-845.296) [-844.416] (-853.968) -- 0:00:05 980000 -- (-850.909) (-840.088) [-843.143] (-841.285) * [-843.934] (-845.122) (-845.702) (-838.726) -- 0:00:05 Average standard deviation of split frequencies: 0.005812 981000 -- (-848.059) (-848.340) [-843.695] (-840.011) * (-844.650) (-843.781) [-841.396] (-842.417) -- 0:00:04 982000 -- [-852.355] (-845.964) (-848.972) (-851.005) * [-850.935] (-846.513) (-848.399) (-842.606) -- 0:00:04 983000 -- [-846.263] (-844.009) (-851.953) (-852.791) * (-848.266) (-839.720) [-842.789] (-846.165) -- 0:00:04 984000 -- [-839.941] (-838.102) (-836.621) (-859.794) * (-862.972) (-841.840) [-843.729] (-839.896) -- 0:00:04 985000 -- (-846.128) (-839.532) (-846.363) [-841.535] * (-855.281) (-840.708) (-844.828) [-843.813] -- 0:00:03 Average standard deviation of split frequencies: 0.006128 986000 -- (-842.275) (-845.226) (-843.730) [-843.000] * [-850.934] (-844.737) (-854.030) (-848.232) -- 0:00:03 987000 -- (-852.281) (-847.499) (-839.151) [-840.245] * (-844.756) [-845.408] (-839.356) (-852.287) -- 0:00:03 988000 -- (-850.859) [-847.239] (-843.576) (-847.065) * [-850.654] (-842.887) (-847.806) (-846.194) -- 0:00:03 989000 -- (-842.019) [-847.415] (-839.740) (-851.093) * (-848.569) (-841.432) [-843.205] (-849.056) -- 0:00:02 990000 -- (-846.622) (-844.389) [-843.477] (-846.258) * (-847.224) (-839.846) (-856.006) [-842.897] -- 0:00:02 Average standard deviation of split frequencies: 0.006662 991000 -- (-849.171) [-842.697] (-843.478) (-844.264) * (-844.627) [-843.338] (-844.664) (-846.405) -- 0:00:02 992000 -- (-849.196) (-850.679) (-838.536) [-844.090] * (-839.112) (-853.687) (-853.016) [-843.104] -- 0:00:02 993000 -- (-859.826) [-839.100] (-841.896) (-843.960) * (-850.961) (-843.958) (-853.927) [-837.012] -- 0:00:01 994000 -- (-848.708) [-853.760] (-847.807) (-840.408) * (-838.616) (-843.518) (-848.013) [-846.126] -- 0:00:01 995000 -- (-849.742) (-842.507) (-846.841) [-844.015] * (-844.554) [-846.266] (-843.245) (-856.198) -- 0:00:01 Average standard deviation of split frequencies: 0.006411 996000 -- (-840.900) (-845.922) (-847.645) [-837.476] * (-842.429) [-842.159] (-855.592) (-847.814) -- 0:00:01 997000 -- [-842.568] (-855.161) (-846.751) (-845.412) * (-844.907) (-848.625) (-846.647) [-841.336] -- 0:00:00 998000 -- (-848.619) (-849.662) (-841.356) [-844.476] * (-843.021) (-845.326) [-846.398] (-848.957) -- 0:00:00 999000 -- (-845.422) [-840.961] (-842.501) (-839.986) * (-841.688) [-838.480] (-846.563) (-837.379) -- 0:00:00 1000000 -- (-843.416) [-843.865] (-846.326) (-851.310) * [-840.771] (-850.854) (-847.311) (-848.665) -- 0:00:00 Average standard deviation of split frequencies: 0.006595 Analysis completed in 4 mins 14 seconds Analysis used 253.75 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -831.65 Likelihood of best state for "cold" chain of run 2 was -831.77 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 63.9 % ( 57 %) Dirichlet(Revmat{all}) 78.5 % ( 77 %) Slider(Revmat{all}) 35.2 % ( 35 %) Dirichlet(Pi{all}) 35.4 % ( 31 %) Slider(Pi{all}) 68.4 % ( 49 %) Multiplier(Alpha{1,2}) 62.5 % ( 38 %) Multiplier(Alpha{3}) 70.5 % ( 43 %) Slider(Pinvar{all}) 26.5 % ( 32 %) ExtSPR(Tau{all},V{all}) 17.5 % ( 20 %) ExtTBR(Tau{all},V{all}) 34.3 % ( 39 %) NNI(Tau{all},V{all}) 37.2 % ( 37 %) ParsSPR(Tau{all},V{all}) 27.0 % ( 23 %) Multiplier(V{all}) 41.8 % ( 34 %) Nodeslider(V{all}) 26.4 % ( 19 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 63.9 % ( 65 %) Dirichlet(Revmat{all}) 77.9 % ( 72 %) Slider(Revmat{all}) 35.1 % ( 33 %) Dirichlet(Pi{all}) 36.4 % ( 32 %) Slider(Pi{all}) 66.9 % ( 44 %) Multiplier(Alpha{1,2}) 62.0 % ( 37 %) Multiplier(Alpha{3}) 70.7 % ( 47 %) Slider(Pinvar{all}) 25.9 % ( 28 %) ExtSPR(Tau{all},V{all}) 17.2 % ( 16 %) ExtTBR(Tau{all},V{all}) 34.1 % ( 30 %) NNI(Tau{all},V{all}) 37.1 % ( 37 %) ParsSPR(Tau{all},V{all}) 27.0 % ( 24 %) Multiplier(V{all}) 41.8 % ( 45 %) Nodeslider(V{all}) 26.5 % ( 24 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.74 0.53 0.37 2 | 166532 0.76 0.56 3 | 167220 166497 0.78 4 | 166626 166745 166380 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.75 0.53 0.37 2 | 166609 0.76 0.56 3 | 167092 166286 0.78 4 | 166071 167274 166668 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -841.63 | 2 1 1 2 | |1 1 2 2 | | 2 2 1 1 2 * | | 1 2 1 1* 2 | | 2 1 121 1 2 22 2 2 2 1 2 2 1 | | 2* 1 222 11 1 2 | | 2 1 1 2 1 11 22 1 * 1 211 22| |2 21 12 1 * 2 121 2 1| | 1 22 1 1 2 1 1 1 | | * 1 2 1 2 1 2 12 | | 1 2 1 2 1 1 | | 2 1 2 | | 2 * 2 2 1 | | 11 1 | | 2 2 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -845.46 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -839.13 -852.69 2 -838.60 -852.39 -------------------------------------- TOTAL -838.83 -852.55 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.307331 0.004585 0.187078 0.438805 0.298793 1154.19 1180.44 1.000 r(A<->C){all} 0.084874 0.001575 0.021467 0.168725 0.079951 371.91 407.28 1.000 r(A<->G){all} 0.156285 0.002580 0.068186 0.260132 0.151139 675.68 729.42 1.002 r(A<->T){all} 0.098169 0.001552 0.027461 0.175485 0.094890 601.38 675.97 1.001 r(C<->G){all} 0.052967 0.001002 0.000809 0.112848 0.048798 405.17 505.00 1.000 r(C<->T){all} 0.547964 0.006146 0.388466 0.691291 0.547392 575.82 625.58 1.000 r(G<->T){all} 0.059741 0.001039 0.005542 0.121671 0.055051 579.79 596.47 1.001 pi(A){all} 0.267685 0.000477 0.224621 0.310232 0.267113 1182.89 1228.95 1.000 pi(C){all} 0.260039 0.000478 0.217422 0.303168 0.259910 1225.25 1262.41 1.000 pi(G){all} 0.226317 0.000462 0.185227 0.269127 0.226020 1311.99 1313.34 1.002 pi(T){all} 0.245959 0.000451 0.203573 0.285478 0.245518 1197.35 1294.89 1.001 alpha{1,2} 0.119799 0.011727 0.000142 0.285446 0.101815 1205.82 1219.59 1.000 alpha{3} 2.339974 1.658014 0.390326 4.916463 2.081782 1312.59 1351.93 1.000 pinvar{all} 0.475264 0.013752 0.244223 0.696565 0.485121 1142.81 1164.09 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C2 3 -- C3 4 -- C4 5 -- C5 6 -- C6 7 -- C7 8 -- C8 9 -- C9 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition --------------- 1 -- .******** 2 -- .*....... 3 -- ..*...... 4 -- ...*..... 5 -- ....*.... 6 -- .....*... 7 -- ......*.. 8 -- .......*. 9 -- ........* 10 -- ..**.**** 11 -- ..**..... 12 -- .....***. 13 -- ..******* 14 -- ..**.***. 15 -- .....**.. 16 -- ..**....* 17 -- ......**. 18 -- .....*.*. 19 -- .....**** 20 -- .*..*.... --------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 10 3002 1.000000 0.000000 1.000000 1.000000 2 11 3002 1.000000 0.000000 1.000000 1.000000 2 12 2986 0.994670 0.000942 0.994004 0.995336 2 13 2450 0.816123 0.001884 0.814790 0.817455 2 14 1228 0.409061 0.005653 0.405063 0.413058 2 15 1048 0.349101 0.016017 0.337775 0.360426 2 16 979 0.326116 0.008951 0.319787 0.332445 2 17 974 0.324450 0.007537 0.319121 0.329780 2 18 972 0.323784 0.007537 0.318454 0.329114 2 19 794 0.264490 0.015075 0.253831 0.275150 2 20 401 0.133578 0.008951 0.127249 0.139907 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.047953 0.000343 0.017494 0.085226 0.045293 1.000 2 length{all}[2] 0.031529 0.000221 0.007388 0.061461 0.029002 1.000 2 length{all}[3] 0.003017 0.000009 0.000001 0.009088 0.002114 1.000 2 length{all}[4] 0.002933 0.000009 0.000001 0.008581 0.002045 1.000 2 length{all}[5] 0.048326 0.000389 0.015827 0.087784 0.044787 1.000 2 length{all}[6] 0.003154 0.000010 0.000000 0.009397 0.002182 1.000 2 length{all}[7] 0.003109 0.000010 0.000002 0.009265 0.002206 1.000 2 length{all}[8] 0.003127 0.000010 0.000000 0.009554 0.002182 1.000 2 length{all}[9] 0.040561 0.000236 0.014767 0.070827 0.038401 1.000 2 length{all}[10] 0.043520 0.000372 0.011782 0.079829 0.040469 1.000 2 length{all}[11] 0.034408 0.000203 0.010503 0.062491 0.031879 1.000 2 length{all}[12] 0.019454 0.000098 0.003296 0.038696 0.017938 1.000 2 length{all}[13] 0.018283 0.000147 0.000193 0.041312 0.015977 1.000 2 length{all}[14] 0.007042 0.000047 0.000004 0.020956 0.004886 0.999 2 length{all}[15] 0.003086 0.000010 0.000008 0.009275 0.002091 0.999 2 length{all}[16] 0.006529 0.000039 0.000003 0.018719 0.004620 0.999 2 length{all}[17] 0.003075 0.000010 0.000001 0.009425 0.002170 0.999 2 length{all}[18] 0.002930 0.000008 0.000004 0.008657 0.002019 0.999 2 length{all}[19] 0.006100 0.000036 0.000005 0.017910 0.004307 1.000 2 length{all}[20] 0.010643 0.000078 0.000024 0.027367 0.008909 0.998 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.006595 Maximum standard deviation of split frequencies = 0.016017 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.000 Clade credibility values: /------------------------------------------------------------------------ C1 (1) | |------------------------------------------------------------------------ C2 (2) | | /------------------ C3 (3) | /-------100-------+ | | \------------------ C4 (4) + | | | /------------------ C6 (6) | | | | /-------100-------+--------99-------+------------------ C7 (7) | | | | | | | \------------------ C8 (8) \--------82-------+ | | \------------------------------------ C9 (9) | \------------------------------------------------------ C5 (5) Phylogram (based on average branch lengths): /---------------------------------- C1 (1) | |---------------------- C2 (2) | | /-- C3 (3) | /-----------------------+ | | \-- C4 (4) + | | | /-- C6 (6) | | | | /------------------------------+------------+-- C7 (7) | | | | | | | \-- C8 (8) \-----------+ | | \----------------------------- C9 (9) | \---------------------------------- C5 (5) |--------------| 0.020 expected changes per site Calculating tree probabilities... Credible sets of trees (39 trees sampled): 50 % credible set contains 5 trees 90 % credible set contains 15 trees 95 % credible set contains 19 trees 99 % credible set contains 26 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,(((C3,C4),(C6,C7,C8),C9),C5))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **9** sequences, **119** codons, and **1** partitions from `/data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -849.66, AIC-c = 1741.61 (21 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.188 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 2.815 * non-synonymous rate = 0.571 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 112 | 1 | 1.521 | 11.711 | Pos. posterior = 0.9321 | | 118 | 1 | 1.418 | 10.740 | Pos. posterior = 0.9419 | ---- ## FUBAR inferred 2 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.13 are expected to be false positives (95% confidence interval of 0-1 )
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=1000, Nseq=9, Len=119 BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNIQYPIKWRCIYT BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAHEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGTLQYPIKWRCIYT *****************::******:***:*******.:* ********* BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTSTEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN *******:*****.***************: ****** *********::* BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTQTSSSSNE BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTETSSSSNE LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTQTSSSSHE HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTQTSSSSHE BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTKTPSSPEE TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTETSSSSDE TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTETSSSSDE TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTETSSSSDE YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 RYDASKSYFYTETSSSSDE ***********:*.**..*
>BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTTAATTCCGCTCAAGAAGGAACCTTGGCTCCTATTCGCCCTACGTACGCCTATCGGCCAGTGCAGGGAAATATTCAGTACCCAATTAAGTGGCGCTGCATCTACACCTTCGCAGGCTACACAGGCACTGCAACCGAGCCAACCAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTCAGGCTTCAAGATGACGGACGACTCGATGGATTTGAGCTTAGACTGCGTTATAGCACAGCCTTCCAGCGAAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGCAAACGTCGTCGTCATCCAATGAA >BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCACTGCTCAACCAGGTTTGGCAGAAACAGGTTAATTCCGCTCAAGAAGGAACCTTGGCTCCTATTCGCCCTACATTTGCCTACAGGCCAGTGCAGGGAAATCTTCAGTGTCCTATTAAGTGGCGCTGCATTTACACATTTGCAGGCTACACAGGCACTTCAACTGAGCCAACCAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGATGACGGACGACTCGATGGATTTGAGCTTAGACTGCGTTATAGCACAGCCTTCCAGCGAAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGGAAACGTCGTCGTCATCCAATGAA >LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTTAATTCCTCTCAAGAAGGAACTTTGGCTCCTGTTCGCCCTACATACGCCTATCGGCCAGTGCAGGGAAATCTTCAGTGTCCTATCAAGTGGCGTTGCATTTACACTTTTGCAGGCTACACAGGTACTGCAACTGAGCCAACTAAAGTATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGAGTATGGACGACTCGATGGATTTGGACTTAGACTGCGTTATAGCACAGCCTTCGAGCACAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGCAAACGTCGTCGTCATCCCATGAA >HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTTAATTCCTCTCAAGAAGGAACTTTGGCTCCTGTTCGCCCTACATACGCCTATCGGCCAGTGCAGGGAAATCTTCAGTGTCCTATCAAGTGGCGTTGCATTTACACTTTTGCAGGCTACACAGGTACTGCAACTGAGCCAACTAAAGTATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGAGTATGGACGACTCGATGGATTTGGACTTAGACTGCGTTATAGCACAGCCTTCGAGCACAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGCAAACGTCGTCGTCATCCCATGAA >BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTTAATTCCGCTCACGAAGGAACTTTGGCTCCTATTCGCCCCACATTTGCCTATCGGCCAGTGCAGGGTAATCTTCAGTGTCCTATCAAGTGGCGCTGCATCTATACTTTTGCAGGCTACACGGGTACTGCAACTGAGCCAACCAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGATGACGGACGACTCGATGGATTTGAACTTAGACTGCGTTATAGCACAGCCTTCCAGCGAAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGAAAACGCCGTCGTCACCCGAAGAA >TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTCAATTCCGCTCAAGAAGGAACTTTGGCTCCTATTCGCCCTACATACGCCTATCGGCCAGTGCAGGGTAACCTTCAGTGTCCTATTAAGTGGCGCTGCATCTACACTTTTGCAGGCTACACAGGCACTGCAACCGAGCCAACTAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGAGTATGGACGACTCGATGGATTTGGACTTAGACTGCGTTATAGCACAGCCTTCGAGCACAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGGAAACGTCGTCGTCATCCGATGAA >TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTCAATTCCGCTCAAGAAGGAACTTTGGCTCCTATTCGCCCTACATACGCCTATCGGCCAGTGCAGGGTAACCTTCAGTGTCCTATTAAGTGGCGCTGCATCTACACTTTTGCAGGCTACACAGGCACTGCAACCGAGCCAACTAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGAGTATGGACGACTCGATGGATTTGGACTTAGACTGCGTTATAGCACAGCCTTCGAGCACAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGGAAACGTCGTCGTCATCCGATGAA >TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTCAATTCCGCTCAAGAAGGAACTTTGGCTCCTATTCGCCCTACATACGCCTATCGGCCAGTGCAGGGTAACCTTCAGTGTCCTATTAAGTGGCGCTGCATCTACACTTTTGCAGGCTACACAGGCACTGCAACCGAGCCAACTAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTTTGCCTTAGGCTTCAAGAGTATGGACGACTCGATGGATTTGGACTTAGACTGCGTTATAGCACAGCCTTCGAGCACAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGGAAACGTCGTCGTCATCCGATGAA >YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ATGGATTACGTGTCGCTGCTCAACCAGGTTTGGCAGAAACAGGTTAATTCCGCTCAAGAAGGGACTTTGGCTCCTATTCGCCCTACATACGCCTACAGGCCAGTGCAGGGAACTCTCCAGTACCCTATTAAGTGGCGCTGCATCTACACTTTTGCAGGCTACACAGGTACTGCTACTGAGCCAACCAAAGCATTAGCAAAACAAGAAGCCGCTAGGAAGGTCTGCCTTAGGCTTCAAGAGTATGGACGACTCGATGGATTTGGACTTAGACTGCGTTATAGCACAGCCTTCGAGCACAATCGTTATGATGCCTCTAAGTCCTATTTCTACACGGAAACGTCGTCGTCATCCGATGAA
>BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNIQYPIKWRCIYT FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN RYDASKSYFYTQTSSSSNE >BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT FAGYTGTSTEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN RYDASKSYFYTETSSSSNE >LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN RYDASKSYFYTQTSSSSHE >HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSSQEGTLAPVRPTYAYRPVQGNLQCPIKWRCIYT FAGYTGTATEPTKVLAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN RYDASKSYFYTQTSSSSHE >BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAHEGTLAPIRPTFAYRPVQGNLQCPIKWRCIYT FAGYTGTATEPTKALAKQEAARKVCLRLQDDGRLDGFELRLRYSTAFQRN RYDASKSYFYTKTPSSPEE >TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN RYDASKSYFYTETSSSSDE >TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN RYDASKSYFYTETSSSSDE >TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGNLQCPIKWRCIYT FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN RYDASKSYFYTETSSSSDE >YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 MDYVSLLNQVWQKQVNSAQEGTLAPIRPTYAYRPVQGTLQYPIKWRCIYT FAGYTGTATEPTKALAKQEAARKVCLRLQEYGRLDGFGLRLRYSTAFEHN RYDASKSYFYTETSSSSDE
Reading sequence file /data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/fasta/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1 Found 9 sequences of length 357 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 5.1% Found 29 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 6 Calculating analytical mean and variance Doing permutation test for PHI Doing permutation test for NSS Doing Permutation test for MAXCHI Writing alignment of polymorphic unambig sites to: Phi.poly.sites Window size is 32 polymorphic sites **p-Value(s)** ---------- NSS: 0.00e+00 (1000 permutations) Max Chi^2: 2.39e-01 (1000 permutations) PHI (Permutation): 1.10e-02 (1000 permutations) PHI (Normal): 6.34e-03
#NEXUS [ID: 4282605921] begin taxa; dimensions ntax=9; taxlabels BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5 TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5 YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ; end; begin trees; translate 1 BY140535_orf4a_AWH65912_1_2014_06_26_China_Unknown_Pipistrellus_bat_coronavirus_HKU5, 2 BY140562_orf4a_AWH65923_1_2014_06_28_China_Unknown_Pipistrellus_bat_coronavirus_HKU5, 3 LMH03f_NS3b_ABN10877_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 4 HKU5_1_LMH03f_NS3b_YP_001039964_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 5 BtPa_GD2013_NA_AIA62345_1_2013_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 6 TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 7 TT07f_NS3b_ABN10904_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 8 TT06f_NS3b_ABN10895_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5, 9 YD13403_orf4a_AWH65934_1_2013_06_03_China_Unknown_Pipistrellus_bat_coronavirus_HKU5 ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:4.529301e-02,2:2.900218e-02,(((3:2.114405e-03,4:2.045229e-03)1.000:3.187905e-02,(6:2.181885e-03,7:2.205907e-03,8:2.181961e-03)0.995:1.793829e-02,9:3.840078e-02)1.000:4.046919e-02,5:4.478693e-02)0.816:1.597721e-02); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:4.529301e-02,2:2.900218e-02,(((3:2.114405e-03,4:2.045229e-03):3.187905e-02,(6:2.181885e-03,7:2.205907e-03,8:2.181961e-03):1.793829e-02,9:3.840078e-02):4.046919e-02,5:4.478693e-02):1.597721e-02); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -839.16 -851.97 2 -838.68 -851.79 -------------------------------------- TOTAL -838.89 -851.88 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.304420 0.004249 0.192208 0.436590 0.295201 867.53 937.34 1.000 r(A<->C){all} 0.087069 0.001616 0.013860 0.160857 0.082410 553.39 620.23 1.000 r(A<->G){all} 0.157403 0.002704 0.069745 0.263839 0.151584 497.65 528.29 1.001 r(A<->T){all} 0.100707 0.001639 0.026705 0.177595 0.096963 349.86 400.34 1.002 r(C<->G){all} 0.053979 0.001010 0.003957 0.117868 0.048972 525.66 679.42 1.001 r(C<->T){all} 0.542941 0.006004 0.404741 0.705339 0.542053 440.86 467.87 1.001 r(G<->T){all} 0.057900 0.001009 0.005587 0.120305 0.052467 398.34 539.83 1.000 pi(A){all} 0.267532 0.000490 0.223611 0.308719 0.267272 1089.42 1190.71 1.001 pi(C){all} 0.260152 0.000492 0.217138 0.303646 0.259480 1168.94 1334.97 1.001 pi(G){all} 0.227061 0.000475 0.186221 0.271200 0.226350 1137.35 1178.08 1.000 pi(T){all} 0.245256 0.000473 0.201398 0.286221 0.245012 1236.80 1282.96 1.000 alpha{1,2} 0.120502 0.011230 0.000061 0.294930 0.100671 1067.70 1113.04 1.000 alpha{3} 2.309624 1.642257 0.473627 4.929037 2.024246 1259.95 1319.39 1.000 pinvar{all} 0.472338 0.013584 0.237283 0.681523 0.479801 1168.29 1183.77 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,(((C3,C4),(C6,C7,C8),C9),C5))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **9** sequences, **119** codons, and **1** partitions from `/data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result/original_alignment/fubar/results/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1/TT03f_NS3b_ABN10886_1_NA_China_Bat_Pipistrellus_bat_coronavirus_HKU5.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -849.66, AIC-c = 1741.61 (21 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.188 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 2.815 * non-synonymous rate = 0.571 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 112 | 1 | 1.521 | 11.711 | Pos. posterior = 0.9321 | | 118 | 1 | 1.418 | 10.740 | Pos. posterior = 0.9419 | ---- ## FUBAR inferred 2 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.13 are expected to be false positives (95% confidence interval of 0-1 )
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500