--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -617.48 -634.77 2 -617.23 -636.92 -------------------------------------- TOTAL -617.34 -636.34 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.178313 0.001050 0.116801 0.239773 0.175271 1303.08 1402.04 1.000 r(A<->C){all} 0.079048 0.002041 0.009457 0.170477 0.071840 467.36 537.39 1.000 r(A<->G){all} 0.225054 0.004421 0.103278 0.355008 0.219789 438.36 505.03 1.001 r(A<->T){all} 0.064725 0.000963 0.013930 0.127309 0.059625 648.09 709.40 1.000 r(C<->G){all} 0.065099 0.002079 0.000015 0.154581 0.055015 502.87 512.51 1.000 r(C<->T){all} 0.439084 0.006526 0.281725 0.595534 0.437984 455.10 550.56 1.001 r(G<->T){all} 0.126991 0.002040 0.042310 0.209926 0.121780 811.66 890.80 1.000 pi(A){all} 0.261889 0.000657 0.210931 0.310406 0.261649 1003.58 1032.23 1.002 pi(C){all} 0.155242 0.000433 0.116419 0.196806 0.154836 1096.47 1124.74 1.000 pi(G){all} 0.209123 0.000563 0.163449 0.255496 0.209300 1063.42 1092.50 1.000 pi(T){all} 0.373746 0.000812 0.319034 0.431979 0.373304 1090.53 1183.59 1.001 alpha{1,2} 1.185373 0.943523 0.003009 3.161392 0.895914 1261.56 1381.28 1.001 alpha{3} 1.318890 0.943571 0.001626 3.242017 1.073839 1117.90 1195.80 1.000 pinvar{all} 0.236886 0.024629 0.000053 0.515873 0.222638 724.47 828.14 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Wed Nov 02 20:10:57 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=1000, Nseq=17, Len=88 C1 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C2 MFNLFLTDTVWYVGQIIFIFAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C3 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C4 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C5 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C6 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C7 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C8 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C9 MFNVLSTDTVWYVGQIIFIVAVCLMVTIVVVAFLASIKLCIQLCGLCNTL C10 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C11 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C12 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C13 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C14 MFNLFLTDTVWYVGQIIFIVAVCLMVTITVVAFLASIKLCIQLCGLCNTL C15 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C16 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C17 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL ***:: ************.******** ********* *********** C1 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C2 VLSPSIYLYDRSKQLYKYYNEEMRMPLLEVDDI----- C3 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C4 LLSPSIYVYNGSKQLYKYYNEEVRPPPLEVDDKIIQTL C5 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C6 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C7 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C8 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C9 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C10 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C11 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C12 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C13 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C14 VLSPSIYLYDRSKQLYKYYNEEVRPPPLEVDDIIIQTL C15 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C16 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C17 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL :***** :*: ***********:* * ***** -- Starting log on Wed Nov 02 20:13:36 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.08 sec, SCORE=999, Nseq=17, Len=88 C1 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C2 MFNLFLTDTVWYVGQIIFIFAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C3 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C4 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C5 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C6 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C7 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C8 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C9 MFNVLSTDTVWYVGQIIFIVAVCLMVTIVVVAFLASIKLCIQLCGLCNTL C10 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C11 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C12 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C13 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C14 MFNLFLTDTVWYVGQIIFIVAVCLMVTITVVAFLASIKLCIQLCGLCNTL C15 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C16 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C17 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL ***:: ************.******** ********* *********** C1 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C2 VLSPSIYLYDRSKQLYKYYNEEMRMPLLEVDDI----- C3 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C4 LLSPSIYVYNGSKQLYKYYNEEVRPPPLEVDDKIIQTL C5 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C6 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C7 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C8 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C9 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C10 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C11 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C12 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C13 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C14 VLSPSIYLYDRSKQLYKYYNEEVRPPPLEVDDIIIQTL C15 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C16 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C17 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL :***** :*: ***********:* * ***** -- Starting log on Wed Nov 02 20:10:57 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=1000, Nseq=17, Len=88 C1 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C2 MFNLFLTDTVWYVGQIIFIFAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C3 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C4 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C5 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C6 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C7 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C8 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C9 MFNVLSTDTVWYVGQIIFIVAVCLMVTIVVVAFLASIKLCIQLCGLCNTL C10 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C11 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C12 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL C13 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C14 MFNLFLTDTVWYVGQIIFIVAVCLMVTITVVAFLASIKLCIQLCGLCNTL C15 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C16 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL C17 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL ***:: ************.******** ********* *********** C1 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C2 VLSPSIYLYDRSKQLYKYYNEEMRMPLLEVDDI----- C3 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C4 LLSPSIYVYNGSKQLYKYYNEEVRPPPLEVDDKIIQTL C5 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C6 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C7 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C8 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C9 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C10 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C11 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C12 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL C13 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C14 VLSPSIYLYDRSKQLYKYYNEEVRPPPLEVDDIIIQTL C15 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C16 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL C17 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL :***** :*: ***********:* * ***** -- Starting log on Wed Nov 02 21:23:17 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/gapped_alignment/fubar,A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 17 taxa and 264 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C10 Taxon 3 -> C11 Taxon 4 -> C12 Taxon 5 -> C13 Taxon 6 -> C14 Taxon 7 -> C15 Taxon 8 -> C16 Taxon 9 -> C17 Taxon 10 -> C2 Taxon 11 -> C3 Taxon 12 -> C4 Taxon 13 -> C5 Taxon 14 -> C6 Taxon 15 -> C7 Taxon 16 -> C8 Taxon 17 -> C9 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1667424199 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 132237691 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 8018085733 Seed = 1849877312 Swapseed = 1667424199 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 18 unique site patterns Division 2 has 14 unique site patterns Division 3 has 23 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -887.305235 -- 54.852219 Chain 2 -- -949.027175 -- 54.852219 Chain 3 -- -948.885224 -- 54.852219 Chain 4 -- -893.239859 -- 54.852219 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -887.447185 -- 54.852219 Chain 2 -- -898.111055 -- 54.852219 Chain 3 -- -902.944346 -- 54.852219 Chain 4 -- -836.294307 -- 54.852219 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-887.305] (-949.027) (-948.885) (-893.240) * [-887.447] (-898.111) (-902.944) (-836.294) 1000 -- [-643.436] (-629.948) (-634.349) (-676.490) * [-629.773] (-632.653) (-652.564) (-653.777) -- 0:00:00 2000 -- (-629.104) (-634.180) [-630.519] (-627.120) * (-623.939) [-626.199] (-633.263) (-639.244) -- 0:00:00 3000 -- (-646.317) (-618.440) [-631.959] (-623.714) * (-643.228) [-624.283] (-631.858) (-637.101) -- 0:05:32 4000 -- (-632.495) [-622.663] (-624.094) (-634.035) * (-636.982) (-622.383) [-622.528] (-634.772) -- 0:04:09 5000 -- (-626.174) (-627.353) [-619.068] (-636.790) * (-632.699) (-631.591) (-622.861) [-623.086] -- 0:03:19 Average standard deviation of split frequencies: 0.106446 6000 -- (-624.365) (-633.243) [-617.978] (-638.123) * (-634.532) [-627.327] (-638.541) (-636.187) -- 0:05:31 7000 -- (-627.627) (-634.417) (-627.908) [-625.221] * (-633.388) [-624.525] (-645.806) (-633.189) -- 0:04:43 8000 -- [-626.283] (-630.438) (-625.913) (-620.848) * (-635.198) [-623.748] (-638.104) (-629.528) -- 0:06:12 9000 -- (-637.505) (-622.958) [-629.198] (-630.671) * (-636.038) [-621.915] (-638.111) (-638.647) -- 0:05:30 10000 -- [-625.379] (-629.977) (-626.764) (-623.521) * (-644.203) (-632.732) (-633.892) [-616.528] -- 0:04:57 Average standard deviation of split frequencies: 0.075224 11000 -- (-631.436) (-634.732) [-625.201] (-634.626) * [-623.770] (-626.164) (-638.277) (-624.556) -- 0:05:59 12000 -- (-625.317) (-634.963) [-625.616] (-625.454) * [-624.210] (-627.054) (-634.690) (-620.648) -- 0:05:29 13000 -- (-619.349) (-632.516) (-628.424) [-617.965] * [-629.866] (-628.514) (-631.809) (-632.551) -- 0:06:19 14000 -- (-626.181) [-627.229] (-615.910) (-629.043) * (-623.136) [-619.189] (-624.025) (-626.824) -- 0:05:52 15000 -- (-628.998) (-624.286) (-633.985) [-621.840] * (-627.056) [-624.777] (-631.166) (-626.111) -- 0:05:28 Average standard deviation of split frequencies: 0.061604 16000 -- [-619.628] (-633.446) (-635.759) (-628.698) * (-622.316) [-628.988] (-626.148) (-634.145) -- 0:06:09 17000 -- (-631.464) (-634.046) (-620.393) [-616.830] * [-627.626] (-642.591) (-628.060) (-622.969) -- 0:05:46 18000 -- (-621.261) [-624.614] (-635.679) (-623.468) * (-625.429) [-628.937] (-624.205) (-626.256) -- 0:06:21 19000 -- (-623.309) (-624.126) (-630.591) [-619.266] * (-618.950) (-626.575) (-627.662) [-626.442] -- 0:06:01 20000 -- (-631.385) [-622.730] (-623.972) (-627.083) * (-626.123) [-628.168] (-629.908) (-624.574) -- 0:05:43 Average standard deviation of split frequencies: 0.061227 21000 -- (-633.989) (-629.581) [-621.505] (-622.048) * (-618.912) (-630.111) (-629.314) [-620.183] -- 0:06:12 22000 -- (-627.517) (-629.359) (-622.431) [-617.323] * (-625.268) (-647.111) [-618.088] (-619.272) -- 0:05:55 23000 -- [-629.566] (-626.844) (-622.747) (-623.886) * (-622.504) (-632.202) [-620.380] (-631.267) -- 0:05:39 24000 -- [-625.701] (-630.247) (-627.591) (-614.925) * (-640.047) (-623.283) [-619.234] (-631.871) -- 0:06:06 25000 -- (-643.945) (-628.787) (-629.064) [-624.039] * [-622.652] (-631.562) (-625.226) (-630.643) -- 0:05:51 Average standard deviation of split frequencies: 0.049105 26000 -- (-639.643) (-624.555) [-627.854] (-616.965) * (-619.906) [-621.722] (-620.847) (-619.316) -- 0:06:14 27000 -- [-614.128] (-640.949) (-625.038) (-626.811) * [-618.704] (-633.304) (-628.667) (-625.847) -- 0:06:00 28000 -- (-633.306) (-626.307) (-628.599) [-628.463] * (-620.330) (-630.579) [-620.619] (-626.335) -- 0:05:47 29000 -- [-616.867] (-616.199) (-616.713) (-626.991) * (-623.826) (-632.887) (-618.179) [-627.693] -- 0:06:08 30000 -- (-625.058) (-634.671) (-618.996) [-621.246] * (-626.126) [-617.115] (-619.129) (-628.458) -- 0:05:55 Average standard deviation of split frequencies: 0.043689 31000 -- (-640.757) (-630.198) (-615.937) [-624.196] * (-627.519) (-620.766) (-623.336) [-624.841] -- 0:06:15 32000 -- (-640.678) (-640.429) [-622.936] (-631.477) * (-623.847) (-630.526) [-618.572] (-614.994) -- 0:06:03 33000 -- (-636.928) (-626.067) [-624.510] (-629.302) * (-640.010) [-614.709] (-627.276) (-620.298) -- 0:05:51 34000 -- (-620.936) (-634.058) (-631.433) [-626.730] * (-626.790) (-628.971) (-631.965) [-617.299] -- 0:06:09 35000 -- (-623.757) [-623.419] (-630.472) (-628.945) * (-630.588) (-630.247) [-620.959] (-617.915) -- 0:05:58 Average standard deviation of split frequencies: 0.040824 36000 -- (-640.584) (-624.158) (-631.503) [-624.410] * [-632.342] (-631.226) (-620.001) (-619.924) -- 0:06:14 37000 -- [-627.855] (-621.939) (-621.469) (-639.771) * (-632.854) (-621.327) (-633.796) [-620.160] -- 0:06:04 38000 -- (-623.840) (-624.909) [-633.823] (-638.305) * (-636.852) (-627.462) [-615.252] (-635.318) -- 0:05:54 39000 -- [-626.133] (-634.401) (-621.793) (-624.577) * (-634.842) (-623.521) [-619.073] (-631.605) -- 0:06:09 40000 -- (-634.139) (-629.003) [-619.084] (-629.629) * (-640.071) [-622.682] (-631.443) (-646.492) -- 0:06:00 Average standard deviation of split frequencies: 0.034776 41000 -- [-620.424] (-625.379) (-624.202) (-649.990) * (-624.588) (-628.309) (-622.663) [-618.631] -- 0:06:14 42000 -- (-622.982) [-625.705] (-626.847) (-631.912) * (-628.571) (-626.263) (-623.597) [-628.321] -- 0:06:04 43000 -- [-619.371] (-630.618) (-627.280) (-640.037) * (-628.107) [-624.061] (-619.105) (-622.958) -- 0:05:56 44000 -- (-621.870) [-619.231] (-630.235) (-637.633) * (-631.946) (-624.554) (-632.045) [-625.663] -- 0:06:09 45000 -- (-627.326) (-620.841) [-622.180] (-636.007) * (-624.360) [-623.008] (-635.350) (-636.658) -- 0:06:00 Average standard deviation of split frequencies: 0.029076 46000 -- (-621.968) [-631.946] (-631.178) (-629.428) * (-629.328) (-631.968) (-636.689) [-618.794] -- 0:06:13 47000 -- (-625.464) (-630.258) (-627.705) [-636.899] * (-633.866) (-624.376) (-626.422) [-627.551] -- 0:06:04 48000 -- [-616.808] (-630.817) (-626.069) (-627.038) * (-635.686) (-626.696) (-626.685) [-617.830] -- 0:05:57 49000 -- (-616.989) (-627.978) [-618.805] (-623.317) * (-632.030) (-628.640) (-633.399) [-630.220] -- 0:06:08 50000 -- (-627.740) (-629.996) [-626.765] (-631.836) * (-634.493) (-627.619) [-622.047] (-633.566) -- 0:06:01 Average standard deviation of split frequencies: 0.030006 51000 -- (-625.469) [-621.746] (-634.773) (-618.894) * (-640.546) (-632.669) [-615.688] (-625.904) -- 0:05:53 52000 -- (-623.648) (-634.986) (-627.613) [-627.186] * (-627.521) (-636.287) [-621.608] (-623.732) -- 0:06:04 53000 -- (-629.219) (-623.247) (-642.439) [-622.030] * (-630.219) (-627.061) [-630.676] (-648.535) -- 0:05:57 54000 -- (-637.391) [-625.923] (-621.431) (-620.433) * (-633.463) (-633.233) [-628.539] (-631.822) -- 0:06:07 55000 -- (-643.559) (-628.329) (-617.631) [-618.385] * (-636.603) [-619.056] (-622.515) (-621.447) -- 0:06:00 Average standard deviation of split frequencies: 0.028798 56000 -- (-637.302) (-632.756) [-622.118] (-624.976) * (-631.473) [-618.999] (-625.612) (-636.978) -- 0:05:54 57000 -- (-633.262) (-629.969) [-625.908] (-626.867) * [-616.361] (-633.416) (-624.851) (-626.784) -- 0:06:03 58000 -- (-624.506) (-620.285) (-621.413) [-620.372] * (-627.961) (-622.036) (-629.540) [-622.302] -- 0:05:57 59000 -- (-631.863) (-625.678) [-625.832] (-627.480) * (-633.053) (-632.941) (-624.599) [-623.567] -- 0:06:06 60000 -- [-620.746] (-628.168) (-630.016) (-631.301) * (-625.178) (-627.501) [-633.151] (-626.722) -- 0:06:00 Average standard deviation of split frequencies: 0.027196 61000 -- (-619.267) (-643.750) (-621.625) [-625.568] * (-628.871) [-627.106] (-627.343) (-637.740) -- 0:05:54 62000 -- (-621.530) (-638.018) (-623.937) [-635.416] * (-624.611) [-623.754] (-640.200) (-628.107) -- 0:06:03 63000 -- (-631.175) (-634.414) [-619.078] (-630.387) * (-643.266) [-615.545] (-622.833) (-631.089) -- 0:05:56 64000 -- (-624.134) (-634.080) (-624.455) [-613.935] * (-631.642) (-620.331) [-623.177] (-626.096) -- 0:06:05 65000 -- (-627.162) (-649.332) [-626.390] (-623.954) * (-619.548) (-628.436) [-626.748] (-633.027) -- 0:05:59 Average standard deviation of split frequencies: 0.026253 66000 -- [-628.534] (-645.109) (-634.405) (-626.212) * (-622.914) [-621.663] (-624.509) (-633.977) -- 0:05:53 67000 -- (-623.719) (-632.660) (-618.818) [-622.551] * (-620.631) (-620.793) [-627.676] (-627.117) -- 0:06:02 68000 -- [-622.729] (-627.142) (-624.598) (-633.375) * (-624.459) (-631.882) (-624.996) [-622.033] -- 0:05:56 69000 -- (-625.953) [-628.651] (-626.413) (-622.216) * (-623.771) (-625.169) (-622.385) [-627.273] -- 0:05:50 70000 -- [-618.291] (-623.943) (-618.600) (-629.560) * (-643.556) (-628.626) (-622.162) [-618.568] -- 0:05:58 Average standard deviation of split frequencies: 0.022300 71000 -- (-629.910) [-625.762] (-617.781) (-621.129) * (-631.941) [-618.896] (-618.578) (-631.304) -- 0:05:53 72000 -- [-624.654] (-628.752) (-621.992) (-627.481) * (-642.436) (-617.987) [-622.284] (-627.393) -- 0:06:00 73000 -- (-645.090) (-623.591) [-619.782] (-628.583) * [-621.300] (-621.138) (-621.165) (-628.411) -- 0:05:55 74000 -- [-630.653] (-626.423) (-632.035) (-627.870) * (-633.199) (-620.830) [-620.321] (-638.597) -- 0:06:02 75000 -- (-622.315) (-634.757) [-618.037] (-631.026) * (-626.033) (-627.863) (-624.333) [-630.029] -- 0:05:57 Average standard deviation of split frequencies: 0.020159 76000 -- [-636.769] (-621.460) (-630.227) (-623.845) * [-624.599] (-628.154) (-629.472) (-637.526) -- 0:05:52 77000 -- (-648.465) [-620.150] (-633.519) (-626.953) * (-628.950) (-623.172) [-624.764] (-631.129) -- 0:05:59 78000 -- (-628.406) [-616.361] (-632.610) (-626.531) * (-630.616) [-620.469] (-624.515) (-625.051) -- 0:05:54 79000 -- (-630.319) (-634.728) (-622.307) [-616.647] * [-631.504] (-631.052) (-622.490) (-617.963) -- 0:05:49 80000 -- (-643.146) (-618.282) [-620.550] (-621.692) * [-621.139] (-621.846) (-631.723) (-623.814) -- 0:05:56 Average standard deviation of split frequencies: 0.016984 81000 -- (-639.241) (-644.465) (-631.926) [-621.724] * (-629.384) (-624.955) (-633.936) [-628.591] -- 0:05:51 82000 -- (-624.724) [-619.460] (-627.814) (-635.903) * (-638.325) (-626.484) [-625.675] (-624.109) -- 0:05:58 83000 -- [-626.864] (-615.858) (-631.347) (-627.277) * (-634.125) (-622.823) (-625.512) [-622.751] -- 0:05:53 84000 -- [-614.530] (-630.561) (-632.189) (-626.000) * (-627.936) [-621.076] (-635.714) (-629.969) -- 0:05:48 85000 -- (-622.637) (-628.738) [-625.636] (-622.801) * [-618.849] (-621.000) (-632.913) (-636.581) -- 0:05:55 Average standard deviation of split frequencies: 0.015531 86000 -- (-628.461) (-622.704) [-627.605] (-630.038) * (-624.385) (-638.741) [-624.370] (-638.269) -- 0:05:50 87000 -- (-637.291) (-624.640) [-617.211] (-624.548) * (-623.360) [-629.005] (-636.159) (-627.804) -- 0:05:56 88000 -- (-631.241) (-628.959) [-621.490] (-623.642) * (-644.014) (-623.325) [-624.729] (-630.678) -- 0:05:52 89000 -- (-630.833) (-627.503) (-625.068) [-624.645] * (-635.711) (-626.265) (-616.983) [-625.723] -- 0:05:48 90000 -- [-622.004] (-641.257) (-626.889) (-627.769) * (-624.073) (-623.198) [-625.795] (-630.261) -- 0:05:53 Average standard deviation of split frequencies: 0.016609 91000 -- (-625.940) (-629.092) (-622.610) [-624.198] * (-617.927) (-627.044) [-622.927] (-626.092) -- 0:05:49 92000 -- (-627.603) (-627.872) [-620.628] (-634.420) * (-621.136) [-623.547] (-623.807) (-625.934) -- 0:05:55 93000 -- (-627.361) (-629.907) [-619.429] (-625.399) * (-635.081) (-627.038) [-631.629] (-626.072) -- 0:05:51 94000 -- (-629.110) (-641.520) [-622.979] (-633.175) * [-625.292] (-633.112) (-633.163) (-623.625) -- 0:05:46 95000 -- (-627.666) (-640.499) [-620.955] (-637.638) * [-617.835] (-625.085) (-634.325) (-622.845) -- 0:05:52 Average standard deviation of split frequencies: 0.015327 96000 -- [-623.628] (-624.954) (-621.125) (-628.233) * (-624.568) (-624.107) [-623.352] (-630.420) -- 0:05:48 97000 -- (-625.343) (-634.545) (-630.091) [-617.941] * (-635.084) [-619.045] (-632.656) (-627.310) -- 0:05:44 98000 -- (-625.888) (-622.694) (-635.251) [-620.998] * (-617.938) [-620.016] (-620.876) (-633.655) -- 0:05:49 99000 -- [-623.306] (-627.565) (-645.473) (-625.699) * (-622.219) [-613.963] (-628.188) (-633.604) -- 0:05:45 100000 -- (-628.617) (-630.708) [-631.157] (-625.424) * (-625.674) [-630.965] (-627.716) (-641.827) -- 0:05:51 Average standard deviation of split frequencies: 0.015219 101000 -- (-618.776) (-622.990) [-625.557] (-620.025) * [-619.303] (-621.370) (-630.868) (-629.558) -- 0:05:47 102000 -- (-619.368) (-631.383) [-620.266] (-622.488) * [-626.097] (-628.866) (-628.515) (-625.419) -- 0:05:43 103000 -- (-620.018) (-628.553) (-632.714) [-617.915] * (-640.749) (-630.898) (-627.032) [-621.698] -- 0:05:48 104000 -- (-615.796) [-629.488] (-637.677) (-617.901) * (-632.949) [-625.579] (-628.162) (-630.133) -- 0:05:44 105000 -- [-614.610] (-630.051) (-636.498) (-635.339) * (-618.945) (-629.083) [-626.973] (-623.291) -- 0:05:49 Average standard deviation of split frequencies: 0.016183 106000 -- (-625.571) (-633.482) (-622.935) [-623.082] * (-621.828) (-637.015) [-622.775] (-628.128) -- 0:05:45 107000 -- (-624.177) (-642.637) (-624.302) [-620.857] * (-623.090) [-621.968] (-627.495) (-623.501) -- 0:05:42 108000 -- (-623.511) (-631.271) [-620.004] (-621.525) * (-632.699) (-633.872) (-630.319) [-624.996] -- 0:05:46 109000 -- (-625.605) (-622.985) (-621.602) [-626.133] * (-634.703) (-627.180) [-622.305] (-619.777) -- 0:05:43 110000 -- [-621.533] (-620.432) (-619.435) (-635.121) * (-626.857) (-625.013) [-620.402] (-621.852) -- 0:05:47 Average standard deviation of split frequencies: 0.015737 111000 -- [-623.288] (-617.781) (-634.203) (-623.291) * [-619.463] (-631.871) (-629.744) (-630.514) -- 0:05:44 112000 -- [-629.938] (-628.963) (-622.773) (-632.169) * [-619.296] (-631.203) (-627.523) (-625.439) -- 0:05:40 113000 -- [-629.465] (-629.898) (-629.022) (-623.258) * (-624.821) [-623.753] (-632.882) (-625.158) -- 0:05:45 114000 -- (-625.500) [-632.004] (-630.140) (-640.402) * (-626.323) (-627.172) (-631.960) [-623.052] -- 0:05:41 115000 -- (-631.722) (-640.276) (-633.919) [-624.475] * (-623.079) [-619.938] (-633.510) (-640.347) -- 0:05:46 Average standard deviation of split frequencies: 0.013585 116000 -- (-624.938) (-629.324) (-633.556) [-631.679] * [-623.638] (-628.981) (-626.388) (-642.037) -- 0:05:42 117000 -- [-624.301] (-637.697) (-631.498) (-624.441) * (-621.332) (-633.982) (-628.099) [-617.520] -- 0:05:39 118000 -- (-623.802) [-617.656] (-634.453) (-621.095) * [-617.411] (-631.195) (-636.202) (-628.012) -- 0:05:43 119000 -- (-629.700) (-621.451) (-623.222) [-629.287] * (-619.742) [-625.144] (-625.444) (-631.202) -- 0:05:40 120000 -- [-622.133] (-633.202) (-619.726) (-633.778) * (-628.298) (-629.644) (-633.658) [-622.210] -- 0:05:44 Average standard deviation of split frequencies: 0.014622 121000 -- [-623.835] (-624.473) (-628.449) (-627.632) * (-626.162) [-635.490] (-621.935) (-634.371) -- 0:05:41 122000 -- (-631.882) (-636.955) [-627.209] (-638.857) * (-626.092) (-646.249) (-622.499) [-629.648] -- 0:05:38 123000 -- (-625.305) (-629.106) [-623.837] (-623.147) * (-627.009) (-629.420) (-628.681) [-621.027] -- 0:05:42 124000 -- (-637.057) (-623.947) [-623.198] (-624.535) * [-621.308] (-631.626) (-638.337) (-633.851) -- 0:05:39 125000 -- (-633.440) (-630.697) [-620.132] (-628.253) * (-621.242) [-625.511] (-633.822) (-629.132) -- 0:05:36 Average standard deviation of split frequencies: 0.015774 126000 -- (-631.347) (-625.294) [-622.927] (-622.120) * [-625.996] (-631.857) (-629.033) (-621.506) -- 0:05:39 127000 -- (-618.306) (-625.627) [-624.388] (-633.140) * (-625.400) [-620.134] (-633.778) (-642.458) -- 0:05:36 128000 -- (-630.494) [-618.660] (-628.262) (-628.918) * (-630.385) (-628.324) (-632.498) [-618.916] -- 0:05:40 129000 -- [-623.253] (-639.354) (-628.999) (-624.867) * (-624.127) (-631.096) (-633.427) [-623.995] -- 0:05:37 130000 -- [-624.103] (-628.960) (-624.883) (-627.592) * [-618.557] (-629.932) (-627.242) (-627.717) -- 0:05:41 Average standard deviation of split frequencies: 0.014130 131000 -- (-623.655) (-641.414) (-627.214) [-624.287] * (-619.739) (-639.737) [-625.426] (-619.297) -- 0:05:38 132000 -- (-618.994) (-634.969) (-629.984) [-626.140] * [-629.914] (-625.225) (-617.172) (-637.717) -- 0:05:35 133000 -- (-624.913) [-635.945] (-631.446) (-622.678) * (-628.690) [-617.002] (-627.693) (-639.128) -- 0:05:38 134000 -- (-618.802) (-617.910) (-622.383) [-626.408] * (-626.727) (-632.350) (-628.466) [-623.009] -- 0:05:36 135000 -- (-635.754) (-619.523) [-631.900] (-623.255) * (-628.476) (-620.893) (-635.792) [-615.148] -- 0:05:33 Average standard deviation of split frequencies: 0.013961 136000 -- (-645.312) (-622.307) (-629.779) [-626.387] * (-633.994) [-621.481] (-640.874) (-621.056) -- 0:05:36 137000 -- (-631.385) (-627.960) (-632.212) [-624.179] * [-623.049] (-621.041) (-632.009) (-621.635) -- 0:05:33 138000 -- (-636.466) [-616.795] (-626.290) (-625.049) * [-623.653] (-627.511) (-627.496) (-624.119) -- 0:05:37 139000 -- (-626.001) [-630.095] (-629.957) (-635.187) * (-620.223) [-625.079] (-630.294) (-631.202) -- 0:05:34 140000 -- (-626.805) [-617.467] (-627.284) (-637.636) * [-619.572] (-629.950) (-634.080) (-618.327) -- 0:05:31 Average standard deviation of split frequencies: 0.012912 141000 -- (-632.324) [-622.324] (-629.347) (-632.874) * [-630.043] (-626.387) (-628.868) (-617.668) -- 0:05:35 142000 -- (-636.767) (-632.756) [-621.953] (-628.008) * (-631.110) (-637.353) (-624.741) [-616.524] -- 0:05:32 143000 -- [-616.530] (-629.755) (-621.522) (-634.517) * (-620.371) [-618.894] (-633.895) (-627.870) -- 0:05:35 144000 -- [-628.062] (-618.490) (-622.014) (-631.387) * (-624.002) (-626.545) (-628.715) [-624.101] -- 0:05:32 145000 -- (-634.214) (-628.332) (-620.588) [-627.126] * (-620.406) [-623.419] (-621.254) (-620.056) -- 0:05:30 Average standard deviation of split frequencies: 0.011966 146000 -- (-631.564) (-635.140) [-627.862] (-630.565) * (-620.590) [-618.056] (-626.306) (-630.937) -- 0:05:33 147000 -- [-616.486] (-625.041) (-634.599) (-625.225) * (-634.475) (-628.633) [-619.646] (-624.997) -- 0:05:30 148000 -- (-624.404) (-622.691) [-628.931] (-628.790) * (-621.917) (-635.224) [-619.399] (-622.666) -- 0:05:28 149000 -- (-633.071) (-641.544) [-623.302] (-627.759) * (-630.411) (-616.144) [-624.685] (-625.215) -- 0:05:31 150000 -- (-629.918) (-635.684) [-615.308] (-634.731) * (-623.676) (-623.874) [-627.992] (-630.297) -- 0:05:28 Average standard deviation of split frequencies: 0.012791 151000 -- (-634.908) (-626.730) (-629.120) [-620.466] * (-627.537) (-635.331) [-625.807] (-637.578) -- 0:05:31 152000 -- (-633.906) (-628.491) (-619.895) [-625.622] * (-629.366) (-621.357) [-624.290] (-621.557) -- 0:05:29 153000 -- (-634.777) (-627.261) [-617.648] (-626.663) * (-633.821) (-620.929) [-625.529] (-634.220) -- 0:05:26 154000 -- (-630.207) (-620.532) (-617.022) [-623.312] * (-629.704) (-621.606) (-621.212) [-626.113] -- 0:05:29 155000 -- (-630.015) (-630.211) [-625.075] (-629.388) * (-617.249) [-621.109] (-623.799) (-629.756) -- 0:05:27 Average standard deviation of split frequencies: 0.011538 156000 -- (-625.580) (-648.517) [-624.147] (-626.340) * (-639.261) [-619.168] (-636.218) (-628.964) -- 0:05:30 157000 -- (-619.682) (-625.451) (-618.801) [-621.130] * (-634.656) (-628.026) (-638.010) [-620.387] -- 0:05:27 158000 -- (-630.563) (-628.098) [-629.690] (-624.187) * (-630.089) (-627.216) (-643.619) [-627.777] -- 0:05:25 159000 -- (-624.258) [-621.304] (-623.331) (-625.373) * (-629.974) (-621.694) (-624.586) [-628.630] -- 0:05:27 160000 -- [-626.623] (-623.050) (-624.404) (-630.972) * (-636.971) (-635.486) [-617.062] (-626.243) -- 0:05:25 Average standard deviation of split frequencies: 0.011828 161000 -- (-619.827) (-625.518) [-631.643] (-624.033) * [-635.197] (-631.780) (-624.263) (-617.831) -- 0:05:23 162000 -- (-629.510) [-624.966] (-632.186) (-623.831) * (-627.407) (-625.894) [-622.294] (-624.791) -- 0:05:25 163000 -- (-622.233) (-632.654) [-625.270] (-623.088) * (-627.091) [-630.410] (-627.748) (-630.680) -- 0:05:23 164000 -- (-626.505) [-621.558] (-622.585) (-629.333) * (-623.473) (-625.936) (-628.007) [-633.855] -- 0:05:26 165000 -- (-618.327) [-623.087] (-628.038) (-626.986) * (-624.584) (-629.986) (-620.809) [-626.948] -- 0:05:23 Average standard deviation of split frequencies: 0.013781 166000 -- (-623.332) (-634.695) (-631.101) [-617.983] * (-620.525) [-627.863] (-628.520) (-639.174) -- 0:05:21 167000 -- [-618.123] (-626.032) (-626.532) (-621.510) * [-625.986] (-626.440) (-626.549) (-626.671) -- 0:05:24 168000 -- (-649.083) (-629.115) (-637.899) [-621.261] * (-625.727) (-623.616) (-622.906) [-613.356] -- 0:05:21 169000 -- (-626.767) [-620.536] (-633.535) (-622.525) * [-623.420] (-631.602) (-640.836) (-623.196) -- 0:05:24 170000 -- (-627.028) [-617.170] (-629.640) (-616.828) * (-620.255) [-619.029] (-626.768) (-620.278) -- 0:05:22 Average standard deviation of split frequencies: 0.013643 171000 -- (-640.613) (-623.919) (-627.436) [-614.837] * (-623.166) (-625.642) (-637.294) [-620.322] -- 0:05:19 172000 -- (-629.333) (-625.538) [-621.983] (-634.266) * [-626.932] (-630.456) (-643.838) (-636.051) -- 0:05:22 173000 -- (-629.392) [-624.757] (-624.147) (-625.054) * (-631.498) [-620.556] (-634.532) (-616.766) -- 0:05:20 174000 -- (-633.023) (-635.879) [-617.521] (-633.372) * (-622.361) (-622.782) (-630.617) [-628.784] -- 0:05:18 175000 -- [-623.289] (-634.860) (-634.501) (-626.405) * (-628.881) (-631.875) [-617.549] (-635.515) -- 0:05:20 Average standard deviation of split frequencies: 0.011738 176000 -- (-625.037) [-628.434] (-632.689) (-619.779) * (-624.292) [-630.415] (-628.081) (-625.144) -- 0:05:18 177000 -- [-617.712] (-620.417) (-635.170) (-619.896) * (-622.951) (-638.702) [-623.050] (-625.565) -- 0:05:20 178000 -- (-623.391) (-627.488) (-622.232) [-620.709] * [-623.667] (-632.659) (-630.732) (-620.090) -- 0:05:18 179000 -- (-627.844) (-638.086) (-631.516) [-622.584] * [-617.389] (-628.687) (-630.935) (-624.161) -- 0:05:16 180000 -- [-619.764] (-638.571) (-627.491) (-621.161) * (-622.298) [-620.419] (-627.712) (-625.226) -- 0:05:18 Average standard deviation of split frequencies: 0.012205 181000 -- [-623.658] (-623.897) (-618.346) (-640.545) * (-626.167) [-623.561] (-625.577) (-636.304) -- 0:05:16 182000 -- (-626.301) (-627.880) [-624.075] (-619.219) * (-626.858) (-625.773) [-621.932] (-615.094) -- 0:05:19 183000 -- [-635.647] (-636.180) (-644.954) (-633.031) * (-634.131) [-617.921] (-633.117) (-622.311) -- 0:05:16 184000 -- (-625.202) (-626.512) [-630.945] (-638.880) * (-629.875) [-624.543] (-626.049) (-621.414) -- 0:05:14 185000 -- (-623.347) [-618.490] (-627.651) (-614.794) * (-630.194) [-626.605] (-635.431) (-614.336) -- 0:05:17 Average standard deviation of split frequencies: 0.012449 186000 -- (-619.226) (-635.601) (-622.606) [-628.776] * (-622.992) [-615.902] (-632.905) (-627.174) -- 0:05:15 187000 -- (-633.138) [-617.876] (-627.216) (-642.737) * [-628.971] (-633.992) (-628.470) (-628.413) -- 0:05:13 188000 -- (-617.806) [-616.869] (-621.167) (-629.788) * (-626.554) [-623.415] (-634.124) (-629.364) -- 0:05:15 189000 -- (-630.682) (-621.955) (-634.637) [-625.725] * (-637.858) [-620.260] (-633.654) (-626.644) -- 0:05:13 190000 -- (-624.887) [-620.261] (-629.765) (-639.428) * (-629.599) (-631.758) [-622.603] (-621.450) -- 0:05:15 Average standard deviation of split frequencies: 0.013036 191000 -- [-622.726] (-632.006) (-625.002) (-623.955) * (-623.041) (-619.377) [-619.369] (-624.856) -- 0:05:13 192000 -- (-626.893) [-624.765] (-629.858) (-621.593) * (-634.036) (-619.297) [-622.801] (-625.720) -- 0:05:11 193000 -- (-626.714) (-628.939) (-632.853) [-623.300] * (-624.135) (-627.496) [-627.541] (-630.398) -- 0:05:13 194000 -- [-622.930] (-640.816) (-624.711) (-625.385) * (-624.459) (-622.749) [-616.485] (-632.477) -- 0:05:11 195000 -- (-624.572) [-627.560] (-629.654) (-626.708) * (-626.021) [-624.691] (-634.763) (-619.499) -- 0:05:13 Average standard deviation of split frequencies: 0.013469 196000 -- [-630.839] (-621.135) (-625.289) (-631.901) * [-623.496] (-633.777) (-629.132) (-633.222) -- 0:05:11 197000 -- [-620.330] (-637.859) (-638.652) (-634.317) * (-626.809) (-638.008) [-621.233] (-628.302) -- 0:05:09 198000 -- (-628.896) (-631.015) [-628.096] (-624.736) * (-623.374) (-624.900) (-621.920) [-621.080] -- 0:05:11 199000 -- [-630.136] (-624.663) (-627.627) (-636.299) * [-620.332] (-620.450) (-624.669) (-621.176) -- 0:05:09 200000 -- [-621.958] (-626.232) (-632.550) (-628.647) * (-627.058) (-626.463) [-621.060] (-626.835) -- 0:05:12 Average standard deviation of split frequencies: 0.012956 201000 -- [-619.108] (-623.618) (-628.234) (-629.512) * (-626.073) (-626.439) (-631.839) [-624.133] -- 0:05:10 202000 -- (-625.151) [-620.544] (-627.773) (-633.607) * (-622.607) (-634.296) [-626.920] (-629.118) -- 0:05:08 203000 -- (-627.967) (-624.741) [-626.761] (-625.156) * (-625.306) (-629.902) (-625.034) [-626.561] -- 0:05:10 204000 -- (-622.482) [-620.466] (-635.936) (-625.463) * [-626.275] (-619.840) (-626.054) (-625.493) -- 0:05:08 205000 -- (-624.949) (-626.635) [-620.915] (-635.539) * (-627.481) [-619.512] (-627.544) (-631.706) -- 0:05:10 Average standard deviation of split frequencies: 0.013476 206000 -- (-636.023) [-627.477] (-627.522) (-635.601) * (-617.718) (-633.021) [-625.634] (-625.206) -- 0:05:08 207000 -- (-621.547) (-626.598) [-624.906] (-634.195) * (-630.077) (-629.309) (-626.704) [-620.747] -- 0:05:06 208000 -- (-625.131) (-634.323) [-633.148] (-637.107) * [-618.735] (-629.580) (-620.390) (-626.413) -- 0:05:08 209000 -- (-629.399) (-629.327) [-625.785] (-626.499) * (-637.977) (-635.643) (-620.263) [-622.743] -- 0:05:06 210000 -- (-634.216) [-614.591] (-629.038) (-623.341) * (-633.057) (-627.482) (-625.965) [-617.707] -- 0:05:08 Average standard deviation of split frequencies: 0.013426 211000 -- (-629.221) (-630.243) (-627.602) [-617.625] * [-623.488] (-637.279) (-619.970) (-624.306) -- 0:05:06 212000 -- (-627.920) (-627.439) (-631.547) [-628.320] * [-626.119] (-619.574) (-624.833) (-628.857) -- 0:05:04 213000 -- (-627.241) (-631.853) [-624.626] (-632.472) * (-625.416) (-618.134) [-619.461] (-629.294) -- 0:05:06 214000 -- (-628.781) [-623.703] (-627.504) (-630.971) * [-630.382] (-634.352) (-622.786) (-624.040) -- 0:05:04 215000 -- [-618.225] (-637.181) (-627.180) (-637.051) * (-624.580) [-621.482] (-622.937) (-622.155) -- 0:05:06 Average standard deviation of split frequencies: 0.013640 216000 -- (-630.348) (-642.418) [-615.515] (-626.451) * (-629.551) (-629.285) (-618.992) [-619.915] -- 0:05:04 217000 -- (-626.696) [-622.175] (-624.786) (-640.250) * (-625.931) (-625.109) [-623.100] (-623.347) -- 0:05:03 218000 -- [-618.339] (-627.386) (-624.651) (-634.895) * (-628.305) [-618.936] (-623.914) (-623.917) -- 0:05:04 219000 -- (-639.117) (-628.969) (-631.588) [-626.660] * (-632.281) (-618.094) (-628.885) [-622.338] -- 0:05:03 220000 -- [-629.718] (-623.515) (-635.371) (-635.128) * (-641.884) (-622.319) (-620.940) [-622.851] -- 0:05:04 Average standard deviation of split frequencies: 0.013489 221000 -- (-631.650) [-619.300] (-629.977) (-646.205) * (-637.167) (-624.375) [-622.093] (-625.433) -- 0:05:03 222000 -- (-638.919) (-637.956) [-629.161] (-634.273) * (-637.400) [-626.051] (-626.859) (-632.191) -- 0:05:01 223000 -- (-620.884) (-625.906) (-628.411) [-635.539] * (-635.937) (-621.513) [-619.406] (-624.289) -- 0:05:03 224000 -- (-631.763) [-623.625] (-628.114) (-630.252) * (-640.535) [-622.420] (-623.742) (-624.929) -- 0:05:01 225000 -- [-623.314] (-626.669) (-633.033) (-639.046) * [-629.053] (-625.555) (-628.224) (-631.500) -- 0:05:03 Average standard deviation of split frequencies: 0.012863 226000 -- [-615.629] (-631.479) (-631.310) (-631.752) * [-633.957] (-634.280) (-620.056) (-628.500) -- 0:05:01 227000 -- [-616.656] (-629.189) (-626.893) (-628.105) * (-630.960) (-632.046) (-621.965) [-619.582] -- 0:04:59 228000 -- (-640.764) (-636.673) (-618.788) [-632.467] * (-630.647) (-625.561) (-625.055) [-624.588] -- 0:05:01 229000 -- (-633.626) (-644.590) [-614.713] (-625.936) * (-635.699) (-626.138) [-626.090] (-624.525) -- 0:04:59 230000 -- (-637.422) (-627.961) (-622.778) [-628.477] * (-627.873) (-615.849) (-637.326) [-622.707] -- 0:04:57 Average standard deviation of split frequencies: 0.013000 231000 -- (-618.645) (-624.144) [-630.275] (-626.531) * (-626.806) (-622.559) [-622.547] (-621.909) -- 0:04:59 232000 -- [-623.547] (-634.106) (-628.767) (-629.719) * (-632.243) (-619.671) (-646.214) [-620.643] -- 0:04:57 233000 -- (-624.120) (-619.537) [-616.823] (-622.090) * (-641.072) (-619.802) [-633.037] (-626.229) -- 0:04:59 234000 -- [-621.608] (-628.150) (-619.270) (-617.878) * (-622.184) [-621.063] (-634.353) (-631.990) -- 0:04:57 235000 -- [-622.143] (-623.462) (-625.579) (-628.805) * (-626.922) (-622.647) (-636.808) [-622.534] -- 0:04:56 Average standard deviation of split frequencies: 0.014553 236000 -- (-619.816) (-633.637) [-618.407] (-626.822) * (-627.168) [-620.089] (-621.192) (-625.221) -- 0:04:57 237000 -- (-626.887) [-620.763] (-632.296) (-635.728) * (-624.846) (-625.373) [-623.696] (-632.717) -- 0:04:56 238000 -- (-626.585) [-624.439] (-626.104) (-627.890) * (-629.730) (-625.812) (-627.907) [-618.329] -- 0:04:57 239000 -- [-629.479] (-641.937) (-624.074) (-624.929) * (-636.352) [-626.069] (-619.012) (-615.259) -- 0:04:56 240000 -- (-632.188) (-632.601) [-618.773] (-627.414) * (-633.571) (-623.416) (-627.115) [-617.687] -- 0:04:54 Average standard deviation of split frequencies: 0.014691 241000 -- (-639.323) [-625.027] (-625.739) (-630.829) * [-623.383] (-626.731) (-630.596) (-627.167) -- 0:04:56 242000 -- (-629.503) [-625.845] (-622.382) (-622.299) * (-620.885) [-627.117] (-637.852) (-620.895) -- 0:04:54 243000 -- (-623.003) [-624.139] (-624.502) (-630.049) * (-625.175) (-632.622) [-625.676] (-628.436) -- 0:04:52 244000 -- [-618.235] (-629.809) (-625.015) (-624.216) * (-646.893) [-636.936] (-628.628) (-628.281) -- 0:04:54 245000 -- (-625.506) (-621.037) (-622.920) [-618.968] * [-624.077] (-633.561) (-618.237) (-634.533) -- 0:04:52 Average standard deviation of split frequencies: 0.014798 246000 -- (-635.620) (-624.368) [-622.163] (-623.146) * (-621.826) (-641.657) (-627.836) [-624.520] -- 0:04:54 247000 -- (-621.416) (-628.121) [-621.523] (-635.171) * [-622.603] (-639.910) (-630.270) (-622.392) -- 0:04:52 248000 -- [-617.156] (-627.689) (-619.952) (-630.015) * [-616.725] (-628.652) (-614.045) (-638.349) -- 0:04:51 249000 -- [-623.005] (-629.395) (-624.274) (-633.809) * (-631.340) (-636.800) [-627.459] (-627.189) -- 0:04:52 250000 -- (-622.900) [-617.685] (-633.435) (-626.733) * (-628.707) [-628.011] (-629.094) (-626.864) -- 0:04:51 Average standard deviation of split frequencies: 0.014784 251000 -- (-620.730) [-622.857] (-630.714) (-633.975) * (-622.982) [-625.401] (-633.453) (-628.910) -- 0:04:52 252000 -- [-618.756] (-633.360) (-626.845) (-616.500) * (-636.647) (-627.303) [-634.365] (-622.297) -- 0:04:50 253000 -- (-623.801) (-630.113) [-620.701] (-628.532) * (-640.223) (-634.604) (-623.364) [-624.531] -- 0:04:49 254000 -- (-623.824) (-623.966) (-626.060) [-625.739] * (-630.753) [-623.016] (-634.001) (-632.392) -- 0:04:50 255000 -- [-623.699] (-625.032) (-628.210) (-627.125) * (-625.828) [-633.335] (-621.306) (-640.248) -- 0:04:49 Average standard deviation of split frequencies: 0.014169 256000 -- (-625.723) (-624.622) (-630.069) [-614.472] * (-639.197) (-635.186) [-628.724] (-623.621) -- 0:04:50 257000 -- (-623.619) (-638.398) [-623.414] (-623.026) * (-633.378) (-620.335) (-614.874) [-619.184] -- 0:04:49 258000 -- (-619.803) [-626.540] (-625.376) (-624.279) * (-621.143) [-625.094] (-626.602) (-625.126) -- 0:04:47 259000 -- [-623.436] (-634.315) (-623.233) (-615.882) * [-612.533] (-631.418) (-624.157) (-622.448) -- 0:04:48 260000 -- (-628.638) (-618.582) [-624.705] (-622.254) * (-623.819) (-620.666) (-630.565) [-628.753] -- 0:04:47 Average standard deviation of split frequencies: 0.013614 261000 -- (-623.222) (-630.162) [-624.807] (-630.242) * (-618.917) (-624.384) (-626.609) [-626.282] -- 0:04:48 262000 -- (-624.150) [-621.921] (-622.880) (-631.033) * (-636.531) [-624.864] (-633.556) (-618.607) -- 0:04:47 263000 -- (-625.673) (-621.638) [-632.023] (-633.712) * [-623.814] (-620.396) (-630.292) (-622.174) -- 0:04:45 264000 -- (-640.307) [-627.879] (-633.381) (-636.316) * [-626.604] (-640.641) (-633.478) (-636.516) -- 0:04:47 265000 -- (-618.704) (-620.066) (-626.794) [-624.584] * (-622.217) (-621.177) [-629.925] (-631.943) -- 0:04:45 Average standard deviation of split frequencies: 0.013685 266000 -- (-619.364) (-618.130) [-625.616] (-626.836) * [-617.839] (-636.696) (-637.575) (-626.433) -- 0:04:46 267000 -- (-622.530) (-633.671) (-624.787) [-623.435] * (-625.556) (-635.588) (-632.088) [-622.010] -- 0:04:45 268000 -- [-624.761] (-620.176) (-628.814) (-619.121) * (-622.594) [-624.562] (-629.588) (-629.505) -- 0:04:44 269000 -- (-627.849) (-623.076) (-632.056) [-626.749] * (-627.577) (-620.394) [-630.546] (-634.426) -- 0:04:45 270000 -- (-624.318) (-646.163) (-618.344) [-622.475] * (-621.144) (-627.204) (-626.985) [-627.983] -- 0:04:43 Average standard deviation of split frequencies: 0.013207 271000 -- [-622.597] (-625.427) (-615.961) (-632.966) * (-630.653) (-631.014) (-628.009) [-623.186] -- 0:04:45 272000 -- (-626.611) (-631.362) (-634.369) [-625.296] * (-637.766) (-627.092) (-625.541) [-623.262] -- 0:04:43 273000 -- (-617.787) [-618.507] (-622.402) (-635.107) * (-638.016) (-627.089) (-632.379) [-619.634] -- 0:04:42 274000 -- [-629.808] (-633.978) (-627.497) (-621.456) * (-638.011) (-644.260) [-619.162] (-620.147) -- 0:04:43 275000 -- (-633.410) (-627.717) [-627.602] (-629.950) * [-625.274] (-629.061) (-631.360) (-620.702) -- 0:04:42 Average standard deviation of split frequencies: 0.013759 276000 -- (-636.714) (-634.817) (-631.034) [-620.151] * (-629.807) (-623.020) (-637.986) [-619.850] -- 0:04:40 277000 -- (-625.905) [-622.658] (-621.543) (-620.545) * (-633.112) (-630.894) (-627.265) [-625.007] -- 0:04:41 278000 -- (-632.844) (-619.312) (-636.573) [-626.060] * (-623.855) [-630.493] (-627.340) (-631.496) -- 0:04:40 279000 -- (-627.572) [-623.014] (-626.897) (-637.791) * [-618.974] (-630.214) (-640.885) (-622.205) -- 0:04:41 280000 -- (-628.466) [-622.797] (-620.482) (-623.095) * [-622.177] (-622.724) (-628.133) (-627.672) -- 0:04:40 Average standard deviation of split frequencies: 0.013343 281000 -- (-627.157) (-620.755) (-632.209) [-618.300] * [-620.575] (-625.851) (-637.676) (-621.191) -- 0:04:41 282000 -- (-633.256) (-622.606) (-625.667) [-616.592] * (-627.749) (-626.303) (-628.268) [-622.025] -- 0:04:40 283000 -- (-628.690) (-632.193) (-621.797) [-628.367] * (-621.949) (-623.422) (-635.976) [-618.680] -- 0:04:41 284000 -- [-629.576] (-619.441) (-621.571) (-622.661) * (-643.514) (-626.812) (-636.610) [-617.630] -- 0:04:39 285000 -- [-619.480] (-626.490) (-628.099) (-627.443) * (-626.861) (-632.880) (-630.590) [-618.974] -- 0:04:38 Average standard deviation of split frequencies: 0.014056 286000 -- [-621.955] (-638.405) (-629.074) (-635.813) * [-624.105] (-633.084) (-622.654) (-625.386) -- 0:04:39 287000 -- (-624.963) (-632.989) (-622.545) [-619.145] * [-625.025] (-632.132) (-623.440) (-642.430) -- 0:04:38 288000 -- (-620.380) (-629.060) (-625.803) [-628.566] * [-620.215] (-626.068) (-626.177) (-629.045) -- 0:04:39 289000 -- [-621.848] (-626.606) (-632.101) (-627.538) * (-629.871) [-623.467] (-635.709) (-623.385) -- 0:04:38 290000 -- (-624.437) (-624.090) [-624.097] (-615.402) * (-628.623) (-635.667) [-621.197] (-629.673) -- 0:04:36 Average standard deviation of split frequencies: 0.014411 291000 -- (-629.819) (-622.073) (-628.420) [-624.859] * (-614.485) (-630.849) [-621.148] (-633.137) -- 0:04:37 292000 -- (-632.784) [-622.422] (-623.950) (-621.006) * (-626.341) (-622.926) (-625.561) [-628.554] -- 0:04:36 293000 -- [-625.491] (-625.531) (-627.519) (-625.753) * [-614.524] (-625.059) (-630.078) (-627.742) -- 0:04:35 294000 -- [-624.115] (-630.183) (-637.015) (-632.801) * [-621.532] (-625.038) (-619.436) (-624.637) -- 0:04:36 295000 -- [-624.984] (-633.407) (-638.326) (-643.315) * [-620.583] (-623.243) (-631.833) (-622.100) -- 0:04:34 Average standard deviation of split frequencies: 0.013316 296000 -- [-614.971] (-634.050) (-623.294) (-627.387) * (-619.792) (-631.082) (-633.316) [-619.809] -- 0:04:35 297000 -- [-625.490] (-626.047) (-624.982) (-624.517) * [-618.920] (-637.945) (-623.429) (-631.030) -- 0:04:34 298000 -- (-625.415) (-630.828) (-626.409) [-626.386] * [-621.226] (-633.406) (-624.687) (-622.982) -- 0:04:33 299000 -- (-628.438) (-631.087) (-628.330) [-619.343] * (-626.674) (-618.826) [-623.784] (-629.186) -- 0:04:34 300000 -- (-632.654) (-621.966) [-624.972] (-625.792) * [-619.604] (-622.787) (-630.450) (-629.458) -- 0:04:33 Average standard deviation of split frequencies: 0.013327 301000 -- [-630.625] (-635.307) (-623.595) (-634.527) * (-626.715) (-626.251) [-627.000] (-622.796) -- 0:04:34 302000 -- [-628.954] (-627.692) (-630.148) (-637.664) * [-623.851] (-635.260) (-621.950) (-631.997) -- 0:04:32 303000 -- [-624.893] (-631.857) (-637.685) (-644.634) * (-629.599) (-635.716) [-627.231] (-619.489) -- 0:04:31 304000 -- (-622.414) [-626.624] (-625.402) (-646.067) * (-623.909) (-627.509) [-615.971] (-639.767) -- 0:04:32 305000 -- (-624.366) (-639.243) (-627.915) [-619.042] * (-632.708) [-622.965] (-626.843) (-635.486) -- 0:04:31 Average standard deviation of split frequencies: 0.013779 306000 -- (-624.691) [-622.574] (-631.548) (-624.060) * (-630.620) (-629.700) (-636.158) [-626.862] -- 0:04:32 307000 -- [-621.921] (-630.878) (-637.835) (-630.077) * (-637.366) (-627.608) (-636.995) [-625.646] -- 0:04:30 308000 -- (-620.224) [-614.669] (-625.243) (-620.739) * (-632.852) (-628.903) (-630.367) [-620.419] -- 0:04:29 309000 -- (-627.741) [-626.828] (-633.078) (-623.923) * (-627.812) [-624.842] (-637.126) (-632.299) -- 0:04:30 310000 -- (-628.683) [-626.159] (-625.973) (-632.831) * [-626.753] (-637.619) (-633.215) (-619.180) -- 0:04:29 Average standard deviation of split frequencies: 0.013614 311000 -- (-632.105) (-627.647) [-630.722] (-628.664) * (-619.015) (-634.158) [-617.341] (-621.001) -- 0:04:30 312000 -- (-634.543) [-624.351] (-630.938) (-636.096) * [-626.282] (-633.751) (-621.515) (-623.534) -- 0:04:29 313000 -- [-621.381] (-625.914) (-643.310) (-625.594) * (-632.038) (-644.513) (-630.622) [-623.116] -- 0:04:27 314000 -- (-618.373) (-625.682) [-617.178] (-631.011) * (-629.476) [-627.687] (-627.035) (-620.932) -- 0:04:28 315000 -- [-616.326] (-622.590) (-630.879) (-640.476) * (-621.831) (-628.727) [-625.775] (-626.276) -- 0:04:27 Average standard deviation of split frequencies: 0.013053 316000 -- [-618.240] (-621.279) (-621.593) (-638.987) * (-631.788) (-651.784) [-622.534] (-620.092) -- 0:04:26 317000 -- [-620.369] (-628.837) (-626.163) (-640.440) * (-623.295) (-623.727) (-641.747) [-618.234] -- 0:04:27 318000 -- (-621.565) [-623.195] (-631.431) (-628.392) * (-629.642) (-632.366) [-627.271] (-626.547) -- 0:04:25 319000 -- [-620.697] (-633.900) (-624.217) (-630.606) * (-620.982) (-629.992) [-615.835] (-640.226) -- 0:04:26 320000 -- (-622.918) (-624.970) [-625.752] (-632.500) * [-618.256] (-628.507) (-627.732) (-635.761) -- 0:04:25 Average standard deviation of split frequencies: 0.013231 321000 -- (-628.955) (-628.119) (-625.068) [-635.717] * (-631.801) (-627.764) (-631.527) [-620.475] -- 0:04:24 322000 -- [-622.220] (-632.476) (-623.138) (-630.188) * (-622.014) (-632.333) (-632.742) [-622.066] -- 0:04:25 323000 -- [-622.436] (-625.113) (-627.947) (-637.066) * (-621.045) [-626.405] (-619.196) (-622.789) -- 0:04:24 324000 -- [-621.316] (-636.880) (-624.782) (-632.644) * (-635.618) (-623.691) (-629.088) [-618.646] -- 0:04:24 325000 -- (-631.825) [-623.801] (-628.588) (-622.161) * (-636.541) [-625.631] (-624.601) (-619.047) -- 0:04:23 Average standard deviation of split frequencies: 0.013336 326000 -- (-630.819) (-634.198) [-626.133] (-628.265) * (-626.947) [-627.121] (-620.967) (-631.804) -- 0:04:22 327000 -- (-620.268) (-637.310) (-626.593) [-629.953] * [-621.375] (-623.834) (-618.452) (-628.780) -- 0:04:23 328000 -- (-619.279) [-623.752] (-632.959) (-633.938) * (-621.727) (-618.608) (-618.426) [-623.804] -- 0:04:22 329000 -- (-621.056) (-633.121) [-621.662] (-642.258) * (-620.404) (-625.461) (-635.793) [-621.277] -- 0:04:23 330000 -- (-635.733) (-639.128) [-619.040] (-619.285) * [-612.105] (-619.402) (-623.872) (-614.540) -- 0:04:21 Average standard deviation of split frequencies: 0.013425 331000 -- (-616.924) (-628.950) (-631.356) [-622.903] * (-623.299) (-644.820) (-628.203) [-623.338] -- 0:04:20 332000 -- (-620.691) (-625.739) (-624.847) [-618.693] * (-630.383) [-619.954] (-629.305) (-623.157) -- 0:04:21 333000 -- (-629.241) (-628.996) [-626.906] (-628.330) * (-623.533) (-623.958) (-618.036) [-624.729] -- 0:04:20 334000 -- (-626.182) [-614.064] (-620.162) (-637.581) * [-628.836] (-623.590) (-625.871) (-629.393) -- 0:04:21 335000 -- [-618.139] (-621.082) (-629.068) (-629.372) * (-614.843) (-631.784) (-626.057) [-615.858] -- 0:04:20 Average standard deviation of split frequencies: 0.013173 336000 -- (-642.401) (-631.631) [-621.567] (-632.367) * (-631.713) (-631.089) [-625.114] (-627.463) -- 0:04:18 337000 -- [-628.247] (-618.832) (-622.140) (-632.137) * [-626.273] (-627.404) (-628.964) (-629.564) -- 0:04:19 338000 -- (-616.795) [-631.604] (-627.238) (-624.917) * [-613.631] (-628.413) (-629.667) (-628.935) -- 0:04:18 339000 -- (-630.238) (-622.077) [-627.458] (-635.853) * (-626.929) (-632.456) [-625.173] (-631.769) -- 0:04:19 340000 -- (-619.037) [-625.025] (-630.508) (-620.133) * (-619.751) (-624.500) (-635.463) [-620.991] -- 0:04:18 Average standard deviation of split frequencies: 0.013300 341000 -- (-618.001) (-629.528) [-629.367] (-625.456) * (-628.348) [-623.088] (-632.164) (-625.607) -- 0:04:17 342000 -- (-633.524) (-627.819) (-620.871) [-622.811] * (-623.373) (-623.550) [-621.231] (-626.614) -- 0:04:17 343000 -- (-639.688) [-639.486] (-630.895) (-624.532) * (-626.854) [-625.197] (-627.798) (-629.396) -- 0:04:16 344000 -- [-622.756] (-626.835) (-629.794) (-629.510) * [-628.122] (-626.193) (-639.581) (-635.155) -- 0:04:17 345000 -- (-626.479) [-616.563] (-620.873) (-628.288) * [-628.560] (-623.416) (-633.071) (-635.869) -- 0:04:16 Average standard deviation of split frequencies: 0.013473 346000 -- (-628.162) [-619.249] (-627.596) (-620.942) * (-625.607) [-619.514] (-628.270) (-630.601) -- 0:04:15 347000 -- [-619.857] (-624.496) (-628.386) (-627.219) * (-625.790) [-632.966] (-629.422) (-631.852) -- 0:04:15 348000 -- (-625.665) (-627.001) [-626.234] (-621.207) * (-639.263) (-629.898) [-618.978] (-630.684) -- 0:04:14 349000 -- (-635.816) (-628.389) (-622.997) [-618.991] * (-634.913) (-631.145) [-616.676] (-623.417) -- 0:04:15 350000 -- [-622.304] (-623.188) (-627.638) (-623.821) * (-624.383) (-627.127) (-622.825) [-625.361] -- 0:04:14 Average standard deviation of split frequencies: 0.013256 351000 -- (-630.207) (-635.306) (-616.489) [-619.976] * (-630.299) [-626.654] (-644.405) (-628.190) -- 0:04:13 352000 -- (-621.425) [-628.513] (-625.610) (-629.528) * (-629.574) [-617.463] (-635.348) (-629.067) -- 0:04:14 353000 -- (-629.969) [-629.509] (-630.689) (-632.379) * (-642.338) (-624.869) [-633.114] (-635.503) -- 0:04:12 354000 -- (-621.612) (-626.020) (-630.022) [-619.967] * (-626.541) [-621.473] (-619.427) (-636.230) -- 0:04:13 355000 -- (-630.541) [-619.674] (-622.630) (-626.585) * (-620.144) [-621.063] (-628.153) (-626.746) -- 0:04:12 Average standard deviation of split frequencies: 0.013278 356000 -- [-628.146] (-623.497) (-622.959) (-635.036) * (-624.517) [-620.104] (-625.821) (-624.954) -- 0:04:11 357000 -- (-631.167) [-629.203] (-628.269) (-617.522) * (-637.328) (-649.982) [-629.554] (-628.906) -- 0:04:12 358000 -- (-617.132) (-625.563) [-618.693] (-622.288) * (-620.525) [-621.365] (-632.031) (-628.783) -- 0:04:11 359000 -- (-623.971) [-626.157] (-634.596) (-629.988) * [-620.445] (-625.188) (-628.657) (-636.653) -- 0:04:09 360000 -- (-633.212) (-627.913) (-630.139) [-623.223] * (-627.626) (-633.174) [-622.115] (-633.131) -- 0:04:10 Average standard deviation of split frequencies: 0.012780 361000 -- [-625.594] (-619.569) (-629.937) (-630.371) * [-617.659] (-636.220) (-633.595) (-623.420) -- 0:04:09 362000 -- (-626.666) [-619.535] (-621.415) (-634.484) * (-637.091) (-625.996) [-624.502] (-631.903) -- 0:04:10 363000 -- (-627.394) (-632.515) [-630.058] (-619.310) * [-624.891] (-637.696) (-627.193) (-610.740) -- 0:04:09 364000 -- (-626.128) (-643.008) (-619.145) [-624.175] * (-635.960) (-623.738) (-623.519) [-621.582] -- 0:04:08 365000 -- [-621.338] (-627.859) (-622.016) (-624.082) * [-617.009] (-637.124) (-627.590) (-629.541) -- 0:04:08 Average standard deviation of split frequencies: 0.012451 366000 -- (-628.162) (-629.589) [-626.619] (-635.120) * (-636.941) (-626.748) (-619.356) [-616.592] -- 0:04:07 367000 -- (-623.045) (-624.829) (-629.017) [-624.645] * (-621.920) (-623.567) [-614.372] (-622.715) -- 0:04:08 368000 -- (-626.308) (-636.089) (-627.260) [-625.310] * (-618.884) [-624.028] (-631.548) (-621.526) -- 0:04:07 369000 -- (-622.635) [-622.376] (-620.821) (-633.898) * [-621.839] (-636.916) (-624.470) (-620.912) -- 0:04:06 370000 -- (-626.023) (-619.055) (-623.526) [-627.763] * (-623.497) [-616.980] (-632.444) (-620.982) -- 0:04:06 Average standard deviation of split frequencies: 0.013354 371000 -- (-625.029) (-630.366) (-628.968) [-619.023] * (-628.976) [-623.355] (-623.514) (-631.461) -- 0:04:05 372000 -- (-626.549) [-629.742] (-631.003) (-628.206) * (-623.934) (-621.511) (-618.365) [-619.457] -- 0:04:04 373000 -- (-627.770) (-628.413) [-618.927] (-622.542) * (-625.536) [-624.447] (-641.038) (-624.901) -- 0:04:05 374000 -- (-623.628) (-633.623) [-619.985] (-626.888) * (-628.349) (-621.536) (-631.195) [-628.103] -- 0:04:04 375000 -- [-618.395] (-630.243) (-632.782) (-626.946) * [-622.015] (-620.675) (-625.796) (-618.967) -- 0:04:05 Average standard deviation of split frequencies: 0.012955 376000 -- (-624.045) [-624.465] (-630.164) (-626.299) * (-617.949) (-632.397) (-626.653) [-623.546] -- 0:04:03 377000 -- (-627.302) (-636.910) (-637.887) [-629.112] * (-630.773) [-623.716] (-625.302) (-624.660) -- 0:04:02 378000 -- (-623.092) (-625.552) (-622.270) [-621.514] * [-626.133] (-615.682) (-626.620) (-623.902) -- 0:04:03 379000 -- [-630.850] (-636.287) (-631.130) (-632.666) * [-623.022] (-643.578) (-632.479) (-624.209) -- 0:04:02 380000 -- (-618.745) (-626.902) (-632.258) [-621.157] * [-614.217] (-627.863) (-629.788) (-629.854) -- 0:04:03 Average standard deviation of split frequencies: 0.013003 381000 -- (-632.626) [-624.739] (-631.418) (-632.151) * (-627.536) (-634.953) [-628.540] (-619.305) -- 0:04:02 382000 -- (-618.605) [-627.411] (-620.039) (-624.671) * (-633.891) (-629.490) (-630.614) [-621.133] -- 0:04:01 383000 -- (-650.080) [-625.200] (-647.143) (-622.600) * (-631.628) (-630.671) [-622.510] (-634.822) -- 0:04:01 384000 -- (-622.095) [-616.840] (-630.755) (-636.594) * (-623.502) (-620.612) (-621.874) [-620.021] -- 0:04:00 385000 -- (-632.383) (-617.304) [-615.202] (-621.701) * (-618.648) (-632.087) (-626.048) [-625.668] -- 0:04:01 Average standard deviation of split frequencies: 0.012586 386000 -- (-629.532) (-622.006) (-631.699) [-623.773] * (-623.051) [-628.652] (-637.944) (-633.794) -- 0:04:00 387000 -- [-633.789] (-623.979) (-623.823) (-628.217) * (-628.733) (-625.461) (-629.007) [-623.918] -- 0:03:59 388000 -- (-629.071) (-624.472) [-624.247] (-624.653) * [-620.276] (-626.689) (-622.147) (-630.590) -- 0:03:59 389000 -- (-633.807) (-633.934) [-625.037] (-626.941) * (-624.613) [-621.910] (-637.165) (-626.754) -- 0:03:58 390000 -- [-618.882] (-639.932) (-633.123) (-635.220) * (-632.092) (-622.033) (-623.322) [-625.191] -- 0:03:59 Average standard deviation of split frequencies: 0.012134 391000 -- (-623.376) (-633.365) (-623.428) [-622.050] * (-614.727) [-618.902] (-623.061) (-633.592) -- 0:03:58 392000 -- (-635.318) (-626.409) (-630.547) [-624.655] * (-625.685) (-628.689) [-627.213] (-626.630) -- 0:03:57 393000 -- (-624.495) [-618.880] (-628.759) (-641.827) * [-623.796] (-631.246) (-626.857) (-618.103) -- 0:03:57 394000 -- [-619.933] (-625.740) (-629.836) (-625.412) * (-633.772) [-629.745] (-635.332) (-628.051) -- 0:03:56 395000 -- (-633.468) [-621.378] (-639.287) (-631.136) * [-620.026] (-621.789) (-627.719) (-625.935) -- 0:03:55 Average standard deviation of split frequencies: 0.012176 396000 -- [-620.102] (-628.033) (-628.922) (-634.976) * (-623.425) (-624.493) (-634.798) [-619.693] -- 0:03:56 397000 -- (-627.055) (-628.096) (-626.147) [-623.030] * (-620.427) (-625.227) (-623.408) [-627.078] -- 0:03:55 398000 -- (-633.429) (-639.569) [-622.090] (-618.838) * [-634.729] (-614.839) (-630.185) (-642.787) -- 0:03:55 399000 -- [-626.341] (-630.198) (-629.251) (-617.923) * (-631.933) [-620.490] (-625.663) (-624.198) -- 0:03:54 400000 -- (-630.771) [-619.088] (-627.423) (-624.191) * (-623.704) (-643.877) [-631.731] (-625.405) -- 0:03:54 Average standard deviation of split frequencies: 0.012236 401000 -- (-642.376) (-621.524) [-617.233] (-626.010) * (-623.309) (-626.833) [-622.495] (-620.098) -- 0:03:54 402000 -- (-632.539) (-629.781) (-637.868) [-620.317] * (-627.990) (-628.047) (-631.065) [-630.693] -- 0:03:53 403000 -- [-625.099] (-627.919) (-624.909) (-627.288) * (-621.627) (-631.383) (-619.118) [-625.439] -- 0:03:54 404000 -- (-623.432) [-624.977] (-620.522) (-635.588) * (-616.593) (-631.355) [-618.376] (-626.747) -- 0:03:53 405000 -- [-623.909] (-621.918) (-627.290) (-623.644) * [-621.683] (-621.550) (-637.050) (-634.604) -- 0:03:52 Average standard deviation of split frequencies: 0.011876 406000 -- (-623.656) (-637.424) (-633.262) [-621.185] * (-622.191) [-627.185] (-633.610) (-617.565) -- 0:03:52 407000 -- [-618.723] (-625.365) (-637.757) (-622.498) * (-640.419) [-635.177] (-626.961) (-615.191) -- 0:03:51 408000 -- [-629.567] (-630.527) (-627.666) (-630.741) * (-628.668) (-628.412) (-627.098) [-622.286] -- 0:03:50 409000 -- (-625.958) (-635.261) [-617.020] (-631.465) * [-621.423] (-624.085) (-642.605) (-628.810) -- 0:03:51 410000 -- (-627.136) (-640.105) (-637.579) [-623.522] * (-627.641) [-617.156] (-633.461) (-623.176) -- 0:03:50 Average standard deviation of split frequencies: 0.011643 411000 -- [-618.015] (-637.166) (-622.418) (-621.707) * (-626.272) (-639.542) (-621.711) [-620.052] -- 0:03:50 412000 -- [-634.224] (-644.515) (-630.289) (-624.864) * [-616.188] (-634.716) (-629.371) (-635.549) -- 0:03:49 413000 -- (-629.662) (-629.485) [-623.975] (-618.632) * (-633.042) (-626.480) [-620.353] (-629.519) -- 0:03:48 414000 -- (-628.093) [-625.886] (-623.132) (-632.476) * (-632.351) (-640.284) (-630.634) [-635.365] -- 0:03:49 415000 -- (-622.308) (-622.555) [-615.466] (-621.437) * (-637.105) [-620.124] (-624.711) (-627.222) -- 0:03:48 Average standard deviation of split frequencies: 0.011656 416000 -- (-624.033) [-618.636] (-637.068) (-626.141) * (-634.699) (-630.454) (-626.765) [-623.104] -- 0:03:48 417000 -- (-624.161) (-632.041) (-633.841) [-621.082] * (-636.355) (-622.507) [-622.122] (-620.265) -- 0:03:47 418000 -- (-624.652) (-624.416) [-620.599] (-628.003) * (-634.459) [-622.916] (-622.753) (-623.621) -- 0:03:46 419000 -- (-630.180) [-631.599] (-628.265) (-631.648) * [-615.371] (-629.781) (-644.602) (-630.954) -- 0:03:47 420000 -- (-626.383) (-629.998) (-621.836) [-623.970] * [-621.848] (-618.012) (-629.458) (-626.746) -- 0:03:46 Average standard deviation of split frequencies: 0.011686 421000 -- [-625.501] (-622.733) (-629.846) (-617.813) * (-629.765) (-632.251) (-628.238) [-617.677] -- 0:03:46 422000 -- (-626.550) [-621.474] (-622.943) (-620.322) * (-636.737) (-624.584) [-619.059] (-630.567) -- 0:03:45 423000 -- [-618.970] (-629.450) (-628.717) (-626.746) * [-622.689] (-643.949) (-620.837) (-620.351) -- 0:03:45 424000 -- [-622.584] (-637.640) (-627.551) (-630.959) * (-646.990) (-627.353) (-635.820) [-623.433] -- 0:03:45 425000 -- (-629.015) (-630.439) (-643.345) [-626.406] * (-620.395) [-623.069] (-626.322) (-628.347) -- 0:03:44 Average standard deviation of split frequencies: 0.012141 426000 -- (-617.503) [-629.078] (-634.174) (-624.189) * (-621.396) (-631.818) [-628.899] (-621.184) -- 0:03:45 427000 -- (-637.800) (-626.115) (-626.975) [-628.916] * (-617.800) (-628.233) [-622.673] (-625.646) -- 0:03:44 428000 -- (-620.167) [-620.935] (-631.722) (-628.681) * (-630.154) (-627.685) (-626.577) [-631.727] -- 0:03:43 429000 -- (-625.336) [-633.090] (-629.664) (-628.266) * (-623.328) [-618.458] (-631.251) (-616.336) -- 0:03:43 430000 -- (-628.226) (-642.222) (-629.874) [-617.898] * (-628.648) (-626.589) [-623.973] (-638.612) -- 0:03:42 Average standard deviation of split frequencies: 0.011415 431000 -- (-635.805) [-617.686] (-616.920) (-624.892) * (-622.708) [-619.160] (-631.059) (-639.587) -- 0:03:41 432000 -- (-624.143) (-623.132) (-627.680) [-625.910] * [-629.706] (-635.249) (-630.570) (-630.923) -- 0:03:42 433000 -- (-634.674) [-619.950] (-625.913) (-621.614) * (-626.165) (-635.012) (-631.151) [-616.494] -- 0:03:41 434000 -- [-618.885] (-635.883) (-618.090) (-631.693) * [-622.281] (-623.693) (-622.114) (-620.114) -- 0:03:41 435000 -- (-627.778) [-625.816] (-641.297) (-628.784) * (-632.176) [-616.394] (-628.984) (-629.221) -- 0:03:40 Average standard deviation of split frequencies: 0.011677 436000 -- (-624.986) (-632.579) [-622.905] (-634.728) * (-625.903) (-627.079) (-627.026) [-619.859] -- 0:03:39 437000 -- (-635.457) (-628.621) [-620.043] (-619.399) * [-623.652] (-631.917) (-627.854) (-625.650) -- 0:03:40 438000 -- (-632.510) (-622.968) [-618.395] (-632.039) * (-627.471) (-630.710) [-629.102] (-620.178) -- 0:03:39 439000 -- (-644.638) [-613.351] (-622.039) (-628.671) * [-619.740] (-627.918) (-633.128) (-621.259) -- 0:03:39 440000 -- (-618.702) [-621.315] (-632.180) (-619.083) * (-631.830) (-629.049) (-626.592) [-621.758] -- 0:03:38 Average standard deviation of split frequencies: 0.011187 441000 -- [-620.107] (-619.425) (-622.397) (-628.350) * (-630.087) (-644.890) [-614.212] (-628.363) -- 0:03:38 442000 -- (-630.814) (-646.857) (-627.295) [-627.193] * [-625.435] (-621.418) (-621.996) (-625.691) -- 0:03:38 443000 -- (-633.075) [-628.140] (-619.611) (-628.259) * (-628.788) (-620.803) (-620.622) [-624.753] -- 0:03:37 444000 -- (-617.390) [-628.528] (-625.449) (-626.318) * (-622.978) [-629.613] (-640.545) (-623.203) -- 0:03:36 445000 -- (-625.573) (-628.334) [-622.908] (-621.781) * (-634.633) (-623.331) [-623.340] (-621.857) -- 0:03:37 Average standard deviation of split frequencies: 0.010962 446000 -- (-636.075) (-628.947) [-612.560] (-622.592) * [-621.070] (-619.033) (-632.760) (-630.543) -- 0:03:36 447000 -- [-627.196] (-617.238) (-630.932) (-624.499) * [-625.731] (-630.243) (-626.227) (-633.392) -- 0:03:36 448000 -- (-622.654) (-640.641) [-617.350] (-621.153) * (-619.036) (-622.267) [-616.267] (-619.336) -- 0:03:35 449000 -- (-621.819) (-629.010) [-618.728] (-628.314) * (-632.721) [-623.945] (-616.564) (-623.124) -- 0:03:34 450000 -- (-615.897) [-629.399] (-619.036) (-622.954) * (-629.980) (-623.390) [-615.916] (-633.971) -- 0:03:35 Average standard deviation of split frequencies: 0.011387 451000 -- (-618.037) (-635.204) (-629.650) [-618.809] * [-622.870] (-639.774) (-624.038) (-625.897) -- 0:03:34 452000 -- (-630.274) (-630.445) [-633.754] (-622.289) * [-616.936] (-628.348) (-636.135) (-626.146) -- 0:03:34 453000 -- (-625.348) (-630.915) [-621.271] (-630.242) * (-628.782) (-626.164) (-627.252) [-636.568] -- 0:03:33 454000 -- [-619.994] (-631.826) (-635.574) (-634.466) * (-636.760) (-619.259) (-633.665) [-624.138] -- 0:03:32 455000 -- (-633.707) (-632.042) (-639.028) [-627.909] * (-644.529) (-623.102) [-621.020] (-632.976) -- 0:03:33 Average standard deviation of split frequencies: 0.011017 456000 -- (-624.081) (-630.660) (-633.452) [-622.265] * [-619.954] (-618.451) (-629.192) (-621.923) -- 0:03:32 457000 -- (-632.627) [-619.883] (-624.853) (-621.693) * [-628.288] (-628.613) (-623.848) (-624.955) -- 0:03:32 458000 -- (-613.354) [-621.478] (-642.821) (-616.818) * [-627.257] (-635.717) (-621.883) (-633.477) -- 0:03:31 459000 -- (-628.785) (-627.677) (-621.783) [-621.836] * [-621.807] (-625.760) (-618.529) (-628.873) -- 0:03:30 460000 -- (-633.787) (-619.188) [-619.682] (-624.451) * (-620.685) (-617.471) (-630.661) [-620.737] -- 0:03:31 Average standard deviation of split frequencies: 0.011497 461000 -- (-626.448) (-619.142) (-623.023) [-615.011] * (-634.145) (-636.181) [-620.663] (-621.166) -- 0:03:30 462000 -- [-631.981] (-626.477) (-624.005) (-624.652) * (-625.640) [-619.966] (-622.533) (-633.734) -- 0:03:30 463000 -- (-621.373) (-635.261) (-641.908) [-614.242] * (-627.956) [-615.602] (-627.173) (-624.399) -- 0:03:29 464000 -- (-631.689) [-624.171] (-634.338) (-625.271) * (-633.144) [-620.891] (-624.632) (-632.621) -- 0:03:29 465000 -- (-618.038) (-633.196) (-626.367) [-622.627] * [-618.612] (-622.592) (-621.498) (-623.466) -- 0:03:29 Average standard deviation of split frequencies: 0.011247 466000 -- (-628.566) [-618.912] (-631.037) (-622.069) * (-625.949) [-625.497] (-622.435) (-627.974) -- 0:03:28 467000 -- (-621.245) [-617.607] (-640.157) (-626.361) * [-615.649] (-627.774) (-622.529) (-639.054) -- 0:03:28 468000 -- [-622.783] (-624.865) (-625.119) (-630.709) * (-622.225) [-619.010] (-621.340) (-626.253) -- 0:03:28 469000 -- (-615.395) (-631.997) (-628.527) [-627.766] * (-640.949) (-634.661) [-633.445] (-622.402) -- 0:03:27 470000 -- (-633.384) (-625.851) (-618.210) [-626.156] * (-624.325) (-627.980) (-624.690) [-630.691] -- 0:03:27 Average standard deviation of split frequencies: 0.011577 471000 -- (-625.855) (-626.238) [-613.884] (-624.605) * [-620.784] (-638.233) (-618.263) (-624.578) -- 0:03:26 472000 -- [-624.617] (-625.903) (-627.263) (-631.065) * (-629.099) (-627.783) (-626.729) [-618.021] -- 0:03:26 473000 -- (-626.810) (-633.613) [-623.399] (-630.880) * [-630.064] (-633.296) (-626.912) (-626.547) -- 0:03:26 474000 -- (-618.874) (-631.999) [-623.034] (-623.606) * (-626.639) (-644.104) (-626.546) [-627.086] -- 0:03:25 475000 -- (-621.495) (-627.881) [-621.102] (-620.206) * (-646.624) (-622.520) [-616.506] (-628.688) -- 0:03:25 Average standard deviation of split frequencies: 0.011709 476000 -- (-617.222) (-625.186) [-626.359] (-625.651) * (-635.489) (-625.308) [-621.953] (-633.047) -- 0:03:24 477000 -- [-615.528] (-633.117) (-633.083) (-629.206) * (-632.105) (-626.319) [-626.461] (-623.100) -- 0:03:23 478000 -- [-618.612] (-633.833) (-624.996) (-626.504) * [-616.655] (-627.834) (-633.425) (-629.478) -- 0:03:24 479000 -- (-627.354) (-637.652) (-623.951) [-623.016] * (-631.171) (-624.268) [-615.152] (-626.974) -- 0:03:23 480000 -- (-628.889) (-628.755) [-624.559] (-619.669) * (-632.243) (-630.038) (-632.124) [-635.996] -- 0:03:23 Average standard deviation of split frequencies: 0.011040 481000 -- [-622.358] (-623.001) (-625.246) (-648.938) * [-623.768] (-619.501) (-625.122) (-628.237) -- 0:03:22 482000 -- [-619.236] (-622.129) (-634.099) (-622.476) * (-642.023) [-631.668] (-618.905) (-620.309) -- 0:03:22 483000 -- (-626.018) (-627.368) (-633.874) [-616.948] * [-624.893] (-616.602) (-630.682) (-630.368) -- 0:03:22 484000 -- (-628.069) (-634.639) (-642.979) [-622.617] * (-624.432) (-633.054) (-627.356) [-622.331] -- 0:03:21 485000 -- (-620.919) (-625.011) (-629.271) [-625.064] * (-631.151) [-619.693] (-630.241) (-627.485) -- 0:03:20 Average standard deviation of split frequencies: 0.010836 486000 -- [-623.777] (-630.118) (-628.535) (-623.618) * (-628.465) (-643.344) (-630.763) [-622.445] -- 0:03:20 487000 -- (-623.135) (-626.361) [-620.157] (-629.836) * (-635.968) (-621.393) (-629.267) [-635.326] -- 0:03:20 488000 -- [-624.844] (-625.108) (-634.308) (-617.990) * (-639.284) (-619.481) [-623.691] (-631.748) -- 0:03:20 489000 -- (-625.567) [-619.464] (-627.927) (-623.627) * (-624.989) [-623.512] (-629.692) (-632.061) -- 0:03:19 490000 -- (-632.628) (-623.520) [-626.365] (-633.709) * (-630.406) (-629.579) [-631.635] (-626.905) -- 0:03:18 Average standard deviation of split frequencies: 0.010733 491000 -- (-625.345) (-628.590) [-615.582] (-627.168) * (-624.375) (-618.152) (-634.546) [-626.882] -- 0:03:19 492000 -- (-620.581) [-630.849] (-624.803) (-637.682) * (-641.691) (-630.560) (-632.706) [-624.481] -- 0:03:18 493000 -- (-625.971) (-628.131) [-625.987] (-633.514) * (-633.193) (-622.386) (-631.790) [-621.123] -- 0:03:18 494000 -- (-636.218) [-622.400] (-622.250) (-639.337) * [-619.041] (-625.018) (-630.870) (-640.407) -- 0:03:17 495000 -- (-628.449) (-635.391) [-618.758] (-621.546) * [-623.536] (-618.770) (-631.357) (-625.699) -- 0:03:16 Average standard deviation of split frequencies: 0.011242 496000 -- [-628.800] (-627.556) (-640.120) (-633.858) * (-625.738) [-624.881] (-620.499) (-630.305) -- 0:03:17 497000 -- (-623.490) (-630.772) [-627.219] (-627.925) * (-633.344) [-633.079] (-622.785) (-630.911) -- 0:03:16 498000 -- (-641.138) [-622.694] (-632.371) (-625.245) * [-616.713] (-633.191) (-627.303) (-627.865) -- 0:03:15 499000 -- [-621.629] (-625.548) (-640.515) (-630.976) * (-623.920) (-620.315) [-620.734] (-630.721) -- 0:03:15 500000 -- [-623.766] (-627.935) (-630.239) (-641.621) * (-634.024) [-621.165] (-625.292) (-622.066) -- 0:03:15 Average standard deviation of split frequencies: 0.011354 501000 -- [-633.759] (-640.230) (-632.072) (-626.902) * [-620.830] (-631.475) (-633.255) (-621.689) -- 0:03:15 502000 -- [-625.011] (-640.667) (-624.791) (-629.583) * [-616.809] (-637.864) (-627.719) (-623.692) -- 0:03:14 503000 -- [-623.695] (-632.705) (-628.682) (-639.395) * (-635.674) (-638.557) (-631.724) [-624.536] -- 0:03:13 504000 -- (-623.231) (-642.764) (-622.862) [-619.322] * (-624.145) (-630.035) [-629.189] (-628.253) -- 0:03:13 505000 -- (-629.124) (-625.007) [-622.613] (-621.092) * (-629.894) (-626.751) [-628.361] (-631.602) -- 0:03:13 Average standard deviation of split frequencies: 0.011073 506000 -- (-634.783) (-625.538) (-628.284) [-620.516] * (-635.813) (-619.380) [-619.954] (-634.257) -- 0:03:13 507000 -- (-634.920) [-620.380] (-633.882) (-617.921) * (-620.277) (-635.332) [-623.968] (-625.845) -- 0:03:12 508000 -- (-622.656) (-637.147) [-627.891] (-625.450) * (-631.350) [-627.380] (-623.385) (-631.496) -- 0:03:11 509000 -- [-623.704] (-626.463) (-616.826) (-623.632) * (-627.293) (-633.489) [-619.747] (-621.265) -- 0:03:11 510000 -- (-629.979) [-619.606] (-623.675) (-624.269) * [-630.259] (-629.651) (-621.619) (-626.092) -- 0:03:11 Average standard deviation of split frequencies: 0.011157 511000 -- (-622.822) [-624.762] (-629.257) (-629.709) * (-625.375) (-639.141) (-619.343) [-622.047] -- 0:03:10 512000 -- (-619.612) (-619.932) [-621.780] (-627.136) * (-624.155) (-625.799) [-629.968] (-629.441) -- 0:03:10 513000 -- (-626.373) (-626.117) (-621.785) [-632.902] * (-625.997) (-631.001) (-626.320) [-630.902] -- 0:03:09 514000 -- (-619.615) (-623.367) [-622.281] (-628.882) * (-627.997) (-623.303) [-624.836] (-629.642) -- 0:03:10 515000 -- [-620.263] (-627.584) (-617.850) (-635.831) * [-623.721] (-625.857) (-623.617) (-622.562) -- 0:03:09 Average standard deviation of split frequencies: 0.011608 516000 -- (-625.450) [-618.297] (-628.201) (-628.556) * (-621.575) (-630.331) [-631.997] (-627.117) -- 0:03:08 517000 -- (-623.931) (-627.168) [-620.788] (-624.804) * [-628.914] (-627.480) (-619.134) (-627.445) -- 0:03:08 518000 -- (-651.315) (-637.330) (-626.677) [-619.362] * [-619.004] (-635.150) (-625.331) (-633.208) -- 0:03:07 519000 -- (-635.749) (-623.339) [-615.660] (-629.325) * [-620.700] (-622.825) (-632.047) (-635.126) -- 0:03:08 520000 -- (-628.815) (-625.387) (-621.300) [-627.162] * [-621.065] (-631.365) (-622.036) (-633.632) -- 0:03:07 Average standard deviation of split frequencies: 0.011797 521000 -- (-632.046) (-631.725) (-620.453) [-636.629] * (-641.859) [-618.912] (-624.608) (-627.759) -- 0:03:06 522000 -- (-642.413) (-619.733) [-626.761] (-627.482) * (-632.104) (-623.444) (-634.571) [-623.322] -- 0:03:06 523000 -- [-625.958] (-617.127) (-632.513) (-623.001) * [-620.181] (-625.511) (-627.894) (-621.815) -- 0:03:06 524000 -- (-620.631) (-616.280) (-624.054) [-622.617] * [-617.871] (-632.037) (-630.644) (-629.088) -- 0:03:06 525000 -- (-622.738) [-617.453] (-629.392) (-625.069) * (-626.892) [-624.243] (-621.285) (-623.979) -- 0:03:05 Average standard deviation of split frequencies: 0.011598 526000 -- (-621.145) (-628.795) (-637.320) [-618.722] * (-620.858) [-630.601] (-626.725) (-637.883) -- 0:03:04 527000 -- (-622.634) [-627.499] (-642.970) (-620.165) * [-621.029] (-624.043) (-643.098) (-623.221) -- 0:03:04 528000 -- [-620.243] (-632.687) (-639.399) (-622.273) * (-640.750) (-627.136) [-619.502] (-629.137) -- 0:03:04 529000 -- (-625.394) [-621.911] (-618.994) (-628.643) * (-627.815) [-622.606] (-628.660) (-622.059) -- 0:03:03 530000 -- (-628.898) (-642.495) [-618.265] (-622.893) * (-622.351) (-640.300) (-624.328) [-618.627] -- 0:03:03 Average standard deviation of split frequencies: 0.011313 531000 -- (-625.711) (-630.409) (-625.079) [-628.090] * (-626.793) (-630.179) [-619.144] (-624.005) -- 0:03:02 532000 -- (-625.489) [-627.267] (-619.362) (-620.099) * (-628.942) (-636.337) [-618.663] (-635.882) -- 0:03:02 533000 -- [-621.356] (-626.055) (-621.960) (-621.761) * (-636.413) (-640.170) [-626.291] (-629.560) -- 0:03:02 534000 -- (-627.288) (-636.237) (-622.199) [-620.198] * [-622.226] (-639.312) (-628.506) (-624.012) -- 0:03:01 535000 -- [-620.944] (-622.390) (-627.791) (-626.798) * (-624.555) [-628.596] (-622.326) (-623.664) -- 0:03:01 Average standard deviation of split frequencies: 0.011257 536000 -- (-626.729) (-620.962) (-619.459) [-619.475] * (-623.155) (-629.538) (-625.229) [-626.932] -- 0:03:00 537000 -- (-621.448) [-621.076] (-640.061) (-637.408) * (-627.935) (-629.191) [-627.582] (-626.876) -- 0:03:01 538000 -- [-620.664] (-635.388) (-621.121) (-626.551) * (-623.425) (-623.637) [-621.885] (-629.072) -- 0:03:00 539000 -- [-626.240] (-628.970) (-628.138) (-637.139) * (-629.225) [-619.549] (-628.673) (-628.986) -- 0:02:59 540000 -- (-626.001) (-631.471) (-631.299) [-630.289] * (-623.102) (-624.549) (-624.802) [-622.588] -- 0:02:59 Average standard deviation of split frequencies: 0.011617 541000 -- (-625.839) (-628.248) (-623.473) [-622.646] * (-643.998) (-634.217) (-631.644) [-626.830] -- 0:02:59 542000 -- [-618.664] (-623.997) (-621.371) (-627.447) * (-621.996) [-620.181] (-625.219) (-632.747) -- 0:02:59 543000 -- (-628.267) [-623.509] (-633.447) (-635.717) * (-617.297) [-638.665] (-619.220) (-625.976) -- 0:02:58 544000 -- (-618.272) (-630.205) [-627.089] (-630.389) * (-624.605) (-627.896) (-615.763) [-621.630] -- 0:02:57 545000 -- (-632.164) (-625.484) (-625.792) [-620.801] * (-623.138) (-620.976) [-618.626] (-627.328) -- 0:02:57 Average standard deviation of split frequencies: 0.011273 546000 -- (-622.301) (-633.127) (-618.574) [-621.243] * [-625.355] (-632.051) (-621.455) (-633.401) -- 0:02:57 547000 -- (-630.116) (-626.084) [-625.235] (-629.461) * (-626.876) (-628.204) (-626.120) [-630.820] -- 0:02:57 548000 -- [-631.397] (-629.351) (-634.418) (-625.270) * [-620.072] (-634.630) (-628.384) (-623.674) -- 0:02:56 549000 -- (-639.153) (-625.020) [-618.771] (-627.228) * (-638.157) [-629.112] (-632.388) (-630.421) -- 0:02:55 550000 -- (-627.385) (-631.838) (-627.166) [-620.533] * [-625.420] (-619.372) (-625.402) (-635.051) -- 0:02:55 Average standard deviation of split frequencies: 0.011202 551000 -- (-631.984) (-634.642) [-615.471] (-630.113) * (-619.217) (-631.765) [-623.096] (-624.067) -- 0:02:55 552000 -- (-636.392) [-628.866] (-625.385) (-622.520) * [-616.865] (-628.092) (-624.689) (-635.772) -- 0:02:55 553000 -- [-620.720] (-624.553) (-622.101) (-630.156) * (-630.788) (-635.281) (-628.941) [-626.740] -- 0:02:54 554000 -- [-621.641] (-619.298) (-626.225) (-626.933) * (-631.575) (-634.386) [-621.185] (-627.253) -- 0:02:53 555000 -- (-625.994) (-618.609) [-628.804] (-632.150) * (-620.120) (-636.653) (-628.047) [-621.703] -- 0:02:53 Average standard deviation of split frequencies: 0.011531 556000 -- (-627.275) [-624.360] (-640.171) (-625.152) * (-622.197) (-619.143) [-620.064] (-631.520) -- 0:02:53 557000 -- (-630.033) (-624.586) (-635.259) [-623.776] * (-634.469) [-619.466] (-633.120) (-625.115) -- 0:02:52 558000 -- [-615.924] (-630.681) (-620.498) (-624.473) * [-619.703] (-619.695) (-631.782) (-635.523) -- 0:02:52 559000 -- [-622.074] (-628.308) (-624.605) (-624.373) * [-617.353] (-635.142) (-629.606) (-626.466) -- 0:02:51 560000 -- [-622.029] (-624.437) (-637.597) (-621.320) * (-631.983) [-625.268] (-622.195) (-647.363) -- 0:02:52 Average standard deviation of split frequencies: 0.011459 561000 -- (-623.620) (-628.839) (-635.719) [-627.145] * [-619.762] (-631.948) (-623.463) (-625.158) -- 0:02:51 562000 -- (-633.516) [-623.993] (-627.858) (-632.189) * (-622.756) (-628.233) (-621.384) [-620.440] -- 0:02:50 563000 -- (-625.677) [-622.694] (-619.789) (-630.726) * (-627.109) [-623.587] (-624.142) (-623.642) -- 0:02:50 564000 -- (-636.560) (-622.033) [-619.802] (-632.028) * (-626.848) (-642.860) [-619.465] (-633.030) -- 0:02:50 565000 -- (-641.262) (-626.735) (-625.897) [-619.388] * (-633.310) (-628.818) (-639.964) [-622.181] -- 0:02:50 Average standard deviation of split frequencies: 0.011303 566000 -- (-630.851) [-626.643] (-633.868) (-622.851) * (-626.913) [-625.949] (-627.521) (-630.022) -- 0:02:49 567000 -- [-621.753] (-630.671) (-626.910) (-629.435) * (-633.307) (-619.497) (-624.131) [-622.821] -- 0:02:48 568000 -- [-622.114] (-620.605) (-626.468) (-623.938) * (-634.570) [-623.648] (-620.100) (-628.351) -- 0:02:48 569000 -- (-626.192) (-631.909) (-623.261) [-616.875] * (-634.972) [-631.447] (-626.729) (-621.224) -- 0:02:48 570000 -- (-623.049) (-622.376) (-628.298) [-625.981] * (-619.525) [-618.641] (-617.541) (-631.070) -- 0:02:47 Average standard deviation of split frequencies: 0.011140 571000 -- [-626.647] (-623.313) (-633.511) (-624.678) * (-630.333) (-634.644) [-617.911] (-620.334) -- 0:02:47 572000 -- (-620.125) [-629.636] (-617.544) (-623.924) * [-624.653] (-619.188) (-626.745) (-636.069) -- 0:02:46 573000 -- (-638.421) [-620.798] (-626.208) (-629.452) * (-623.809) (-619.882) [-626.392] (-624.470) -- 0:02:46 574000 -- (-623.095) (-622.167) [-620.068] (-631.476) * (-623.002) (-624.174) (-627.543) [-617.359] -- 0:02:46 575000 -- (-627.181) [-616.691] (-625.918) (-631.624) * [-621.206] (-625.121) (-628.033) (-636.399) -- 0:02:45 Average standard deviation of split frequencies: 0.010920 576000 -- (-625.649) (-626.814) (-613.480) [-626.778] * [-629.528] (-631.845) (-624.711) (-625.228) -- 0:02:45 577000 -- (-627.269) (-624.022) (-627.115) [-628.672] * (-632.951) [-623.029] (-637.059) (-630.972) -- 0:02:44 578000 -- [-617.039] (-623.892) (-625.844) (-633.005) * [-628.044] (-632.017) (-627.500) (-624.688) -- 0:02:45 579000 -- (-625.217) [-631.374] (-644.638) (-631.482) * (-635.037) (-628.900) [-626.117] (-627.125) -- 0:02:44 580000 -- (-621.309) (-630.492) [-621.992] (-629.483) * (-628.687) [-617.042] (-625.110) (-624.200) -- 0:02:43 Average standard deviation of split frequencies: 0.010345 581000 -- [-617.815] (-621.460) (-632.237) (-639.579) * (-638.444) [-623.015] (-619.619) (-624.911) -- 0:02:43 582000 -- [-622.040] (-626.243) (-629.083) (-629.918) * (-627.501) [-620.303] (-618.906) (-632.369) -- 0:02:43 583000 -- (-627.225) (-632.789) [-634.549] (-632.479) * [-623.744] (-627.832) (-636.995) (-629.477) -- 0:02:43 584000 -- (-632.041) (-625.038) [-619.896] (-635.108) * [-624.510] (-628.193) (-627.227) (-632.658) -- 0:02:42 585000 -- (-619.835) (-632.188) [-622.871] (-627.043) * (-627.941) (-628.819) (-628.447) [-626.793] -- 0:02:41 Average standard deviation of split frequencies: 0.010412 586000 -- (-626.117) [-623.368] (-618.550) (-637.318) * (-632.020) (-629.757) (-624.436) [-621.230] -- 0:02:41 587000 -- [-623.223] (-630.869) (-623.242) (-632.578) * (-624.229) (-626.717) [-616.899] (-625.991) -- 0:02:41 588000 -- (-631.179) (-636.040) [-621.491] (-630.051) * [-622.613] (-627.652) (-617.904) (-626.007) -- 0:02:40 589000 -- (-630.393) (-625.844) [-618.817] (-623.548) * [-618.284] (-613.647) (-630.555) (-630.355) -- 0:02:40 590000 -- [-622.501] (-636.396) (-629.719) (-626.283) * (-624.873) (-615.644) [-623.141] (-625.467) -- 0:02:39 Average standard deviation of split frequencies: 0.010580 591000 -- (-630.124) (-639.101) [-627.436] (-627.570) * (-618.215) (-625.859) (-629.030) [-621.159] -- 0:02:39 592000 -- [-618.060] (-632.574) (-621.021) (-622.315) * (-622.318) [-626.556] (-629.611) (-626.566) -- 0:02:39 593000 -- (-626.156) [-624.379] (-622.118) (-624.660) * (-617.438) (-633.582) [-616.511] (-638.202) -- 0:02:38 594000 -- (-638.089) (-616.767) [-622.820] (-626.878) * [-622.047] (-637.812) (-628.020) (-629.353) -- 0:02:38 595000 -- (-622.313) [-617.343] (-619.800) (-628.694) * [-621.051] (-634.769) (-628.508) (-628.066) -- 0:02:37 Average standard deviation of split frequencies: 0.010701 596000 -- (-635.199) (-627.068) [-624.364] (-633.995) * (-617.577) (-627.394) (-626.958) [-615.927] -- 0:02:37 597000 -- (-621.220) (-623.267) [-628.221] (-637.381) * (-626.958) [-623.275] (-633.154) (-632.417) -- 0:02:37 598000 -- [-618.189] (-616.587) (-623.484) (-631.527) * [-619.619] (-629.862) (-650.772) (-624.711) -- 0:02:36 599000 -- (-627.339) [-624.098] (-629.699) (-633.315) * (-634.855) (-628.268) (-631.374) [-631.249] -- 0:02:36 600000 -- (-636.566) [-621.714] (-629.871) (-630.501) * (-620.159) (-633.387) (-634.972) [-614.742] -- 0:02:36 Average standard deviation of split frequencies: 0.010875 601000 -- (-629.469) (-621.608) [-625.378] (-623.503) * (-627.569) [-625.443] (-624.240) (-619.768) -- 0:02:35 602000 -- [-626.268] (-631.404) (-627.720) (-621.299) * (-624.352) (-630.032) (-622.460) [-620.417] -- 0:02:35 603000 -- (-631.409) (-625.926) [-621.444] (-629.295) * (-622.790) (-635.519) [-632.300] (-618.197) -- 0:02:34 604000 -- (-622.592) [-632.955] (-629.003) (-638.894) * (-639.573) (-632.867) [-622.255] (-627.094) -- 0:02:34 605000 -- (-622.236) (-629.605) (-634.187) [-630.174] * (-632.012) (-644.205) (-623.577) [-621.877] -- 0:02:34 Average standard deviation of split frequencies: 0.010668 606000 -- [-620.873] (-623.984) (-621.433) (-624.413) * (-629.358) [-626.265] (-623.792) (-632.647) -- 0:02:33 607000 -- [-628.787] (-629.996) (-629.823) (-625.607) * (-639.651) [-618.847] (-626.216) (-636.185) -- 0:02:33 608000 -- [-618.612] (-639.913) (-623.566) (-628.329) * (-625.062) (-627.589) (-632.210) [-625.344] -- 0:02:32 609000 -- [-625.333] (-621.879) (-619.917) (-627.618) * (-629.399) (-629.960) [-620.256] (-629.580) -- 0:02:32 610000 -- [-619.706] (-635.197) (-628.822) (-626.345) * (-626.866) (-625.486) (-636.166) [-624.423] -- 0:02:32 Average standard deviation of split frequencies: 0.009925 611000 -- [-617.571] (-623.715) (-628.604) (-640.544) * (-631.251) (-623.317) [-623.718] (-626.211) -- 0:02:31 612000 -- (-629.663) (-620.766) (-626.110) [-629.475] * (-627.486) [-618.745] (-619.911) (-621.723) -- 0:02:31 613000 -- [-621.631] (-631.257) (-632.933) (-629.377) * (-623.813) [-618.807] (-628.617) (-635.326) -- 0:02:30 614000 -- (-627.466) [-622.967] (-619.327) (-626.886) * (-632.887) (-631.251) (-634.641) [-617.467] -- 0:02:30 615000 -- (-628.342) (-624.551) [-623.329] (-637.712) * (-622.235) (-624.571) (-631.019) [-624.488] -- 0:02:30 Average standard deviation of split frequencies: 0.009599 616000 -- (-622.401) (-634.971) [-627.655] (-629.588) * [-624.783] (-627.882) (-645.379) (-627.648) -- 0:02:29 617000 -- [-627.878] (-616.182) (-628.913) (-638.930) * (-628.972) [-624.768] (-641.511) (-626.579) -- 0:02:29 618000 -- (-629.057) [-617.050] (-623.895) (-632.374) * (-639.318) (-626.872) [-621.743] (-622.025) -- 0:02:28 619000 -- [-621.983] (-625.364) (-629.595) (-641.946) * [-629.997] (-617.454) (-625.839) (-627.909) -- 0:02:28 620000 -- (-639.142) [-621.521] (-628.741) (-628.864) * (-626.522) [-617.171] (-630.584) (-622.239) -- 0:02:28 Average standard deviation of split frequencies: 0.010097 621000 -- (-621.045) [-623.811] (-632.531) (-623.585) * (-629.506) (-623.045) (-629.138) [-619.016] -- 0:02:27 622000 -- (-619.995) [-618.350] (-627.647) (-629.295) * (-638.428) [-627.140] (-623.227) (-625.222) -- 0:02:27 623000 -- (-632.645) (-632.370) (-630.654) [-620.162] * (-631.610) [-618.174] (-622.157) (-633.982) -- 0:02:27 624000 -- (-629.730) (-627.739) [-627.531] (-626.254) * (-621.138) (-618.611) (-618.279) [-619.143] -- 0:02:26 625000 -- (-633.447) (-632.757) (-625.728) [-621.440] * (-640.360) (-620.135) [-623.118] (-619.519) -- 0:02:26 Average standard deviation of split frequencies: 0.009834 626000 -- (-646.499) (-633.193) [-633.690] (-623.855) * (-622.349) [-633.580] (-640.302) (-623.197) -- 0:02:25 627000 -- [-632.154] (-626.921) (-623.899) (-626.810) * (-625.340) [-626.692] (-627.248) (-627.505) -- 0:02:25 628000 -- (-628.706) (-623.611) [-631.355] (-639.924) * (-635.939) (-626.960) (-639.034) [-624.662] -- 0:02:25 629000 -- (-618.827) (-631.788) (-621.539) [-620.477] * (-626.801) [-620.828] (-626.634) (-633.463) -- 0:02:24 630000 -- (-622.819) (-637.497) [-618.254] (-623.747) * (-636.334) (-633.587) [-625.762] (-629.503) -- 0:02:24 Average standard deviation of split frequencies: 0.009783 631000 -- (-623.728) (-632.718) (-633.014) [-621.458] * (-632.552) [-633.642] (-632.116) (-619.968) -- 0:02:23 632000 -- (-622.401) (-625.955) [-623.420] (-632.830) * (-623.900) (-622.666) [-621.424] (-626.663) -- 0:02:23 633000 -- [-621.158] (-630.502) (-631.082) (-621.376) * (-626.322) [-621.369] (-619.793) (-630.344) -- 0:02:23 634000 -- (-634.285) [-623.635] (-629.659) (-636.535) * [-627.571] (-628.651) (-626.558) (-629.887) -- 0:02:22 635000 -- (-626.976) (-621.297) (-621.397) [-624.728] * (-620.051) [-624.091] (-623.751) (-621.459) -- 0:02:21 Average standard deviation of split frequencies: 0.009679 636000 -- (-627.529) (-631.167) (-624.633) [-621.809] * (-623.114) (-629.467) (-631.631) [-614.700] -- 0:02:21 637000 -- (-640.573) (-619.889) (-623.696) [-626.293] * (-640.905) [-626.824] (-623.454) (-620.673) -- 0:02:21 638000 -- [-618.986] (-635.909) (-633.447) (-631.251) * [-623.575] (-618.954) (-626.008) (-631.889) -- 0:02:21 639000 -- [-620.594] (-628.179) (-623.648) (-635.487) * [-620.566] (-620.454) (-622.670) (-633.642) -- 0:02:20 640000 -- (-628.690) [-623.635] (-627.777) (-626.561) * (-618.173) [-624.235] (-632.032) (-629.269) -- 0:02:20 Average standard deviation of split frequencies: 0.009609 641000 -- (-634.292) (-632.282) [-618.917] (-634.935) * (-625.548) (-614.853) [-618.745] (-630.338) -- 0:02:20 642000 -- (-628.892) (-627.191) [-626.116] (-643.449) * [-618.347] (-629.141) (-618.892) (-628.396) -- 0:02:19 643000 -- (-623.841) [-618.862] (-630.328) (-635.061) * (-624.563) (-619.880) (-636.192) [-620.656] -- 0:02:19 644000 -- (-635.271) (-626.146) (-627.498) [-622.871] * (-624.940) (-623.012) (-624.957) [-623.475] -- 0:02:18 645000 -- (-623.404) (-622.439) (-618.387) [-618.085] * [-631.271] (-635.017) (-626.718) (-636.842) -- 0:02:18 Average standard deviation of split frequencies: 0.009507 646000 -- (-637.907) (-636.379) (-619.289) [-627.133] * (-629.994) [-620.318] (-622.935) (-632.661) -- 0:02:18 647000 -- (-626.083) (-625.870) [-623.169] (-622.795) * [-627.209] (-623.266) (-629.198) (-634.823) -- 0:02:17 648000 -- (-629.804) (-631.675) (-628.330) [-624.507] * [-623.499] (-620.431) (-619.291) (-631.846) -- 0:02:16 649000 -- (-629.706) [-623.016] (-632.710) (-631.101) * [-620.833] (-630.253) (-645.579) (-631.180) -- 0:02:16 650000 -- [-621.801] (-622.543) (-620.569) (-625.886) * (-620.155) (-623.016) (-629.897) [-617.781] -- 0:02:16 Average standard deviation of split frequencies: 0.009481 651000 -- (-627.944) [-632.417] (-617.734) (-629.806) * (-620.674) [-621.347] (-633.397) (-622.174) -- 0:02:16 652000 -- [-619.757] (-631.314) (-629.357) (-633.841) * (-626.518) [-622.645] (-624.829) (-633.287) -- 0:02:15 653000 -- (-626.062) [-617.677] (-622.246) (-626.454) * (-631.742) (-627.884) (-629.661) [-626.233] -- 0:02:14 654000 -- (-627.118) [-621.361] (-626.496) (-635.861) * (-630.377) [-617.998] (-633.925) (-623.992) -- 0:02:14 655000 -- [-622.698] (-618.215) (-618.707) (-625.808) * (-636.785) (-625.031) (-629.313) [-624.119] -- 0:02:14 Average standard deviation of split frequencies: 0.009568 656000 -- (-622.081) [-626.247] (-623.277) (-621.341) * (-633.995) (-631.479) [-627.664] (-622.721) -- 0:02:14 657000 -- (-626.610) (-632.760) [-625.739] (-630.263) * (-630.029) (-619.764) [-625.586] (-622.677) -- 0:02:13 658000 -- (-625.635) (-636.952) [-622.891] (-641.353) * (-638.044) (-628.331) [-621.113] (-629.755) -- 0:02:13 659000 -- (-637.571) (-626.378) (-631.224) [-634.516] * [-625.622] (-623.109) (-626.986) (-625.908) -- 0:02:12 660000 -- [-622.605] (-625.613) (-628.853) (-629.978) * (-621.520) (-634.307) (-619.469) [-622.517] -- 0:02:12 Average standard deviation of split frequencies: 0.009255 661000 -- [-622.465] (-638.979) (-627.171) (-619.684) * (-619.748) [-615.872] (-643.264) (-629.934) -- 0:02:12 662000 -- [-633.512] (-627.844) (-628.567) (-630.362) * (-629.012) (-634.578) [-618.835] (-620.478) -- 0:02:11 663000 -- (-630.008) (-646.863) (-631.238) [-612.737] * (-620.523) [-620.670] (-628.808) (-625.879) -- 0:02:11 664000 -- (-635.425) (-626.140) (-622.936) [-621.504] * (-634.005) (-633.870) [-619.192] (-627.259) -- 0:02:11 665000 -- (-633.355) (-623.718) (-627.440) [-621.296] * (-627.516) (-635.433) (-641.174) [-621.946] -- 0:02:10 Average standard deviation of split frequencies: 0.008918 666000 -- (-625.466) [-620.593] (-620.563) (-627.392) * (-634.556) (-626.052) (-622.942) [-622.703] -- 0:02:10 667000 -- (-630.429) [-621.981] (-620.966) (-628.598) * (-623.072) (-633.897) [-626.729] (-629.153) -- 0:02:09 668000 -- (-632.538) [-618.307] (-627.251) (-622.025) * (-623.385) (-635.978) [-629.412] (-624.676) -- 0:02:09 669000 -- (-629.378) (-625.473) (-643.416) [-624.073] * (-615.943) [-627.816] (-626.571) (-623.605) -- 0:02:09 670000 -- (-631.987) (-632.847) (-628.734) [-617.865] * (-631.688) (-627.700) [-621.179] (-629.226) -- 0:02:08 Average standard deviation of split frequencies: 0.008736 671000 -- (-626.930) (-630.991) [-629.400] (-628.686) * [-626.085] (-626.291) (-638.889) (-626.064) -- 0:02:07 672000 -- [-626.032] (-628.333) (-633.425) (-627.455) * (-628.228) (-628.388) [-626.839] (-628.757) -- 0:02:07 673000 -- (-630.893) (-624.694) (-631.666) [-625.595] * [-623.831] (-623.155) (-620.304) (-626.492) -- 0:02:07 674000 -- [-620.759] (-622.761) (-628.493) (-632.890) * [-620.899] (-631.982) (-626.237) (-618.626) -- 0:02:07 675000 -- (-632.951) (-629.800) [-628.221] (-624.399) * (-626.825) [-620.846] (-625.821) (-626.325) -- 0:02:06 Average standard deviation of split frequencies: 0.009185 676000 -- (-632.475) (-621.863) (-623.103) [-618.575] * (-636.289) (-621.179) [-632.980] (-619.001) -- 0:02:06 677000 -- [-623.281] (-620.566) (-618.150) (-623.934) * (-628.979) [-617.247] (-632.786) (-623.113) -- 0:02:05 678000 -- [-622.652] (-620.628) (-620.085) (-624.547) * (-621.187) [-627.278] (-626.610) (-629.466) -- 0:02:05 679000 -- [-620.873] (-622.732) (-628.829) (-622.354) * (-641.751) (-639.550) (-628.523) [-615.881] -- 0:02:05 680000 -- (-625.283) [-624.233] (-621.465) (-630.034) * (-634.883) [-619.362] (-630.646) (-620.650) -- 0:02:04 Average standard deviation of split frequencies: 0.009142 681000 -- (-627.374) (-631.013) (-619.557) [-623.436] * (-622.222) (-622.295) (-630.338) [-632.041] -- 0:02:04 682000 -- (-624.143) (-624.639) (-627.546) [-628.113] * [-625.740] (-625.197) (-637.146) (-620.607) -- 0:02:04 683000 -- [-623.323] (-625.500) (-622.806) (-637.552) * (-624.570) [-615.113] (-620.652) (-629.830) -- 0:02:03 684000 -- [-625.512] (-625.418) (-627.672) (-635.555) * (-625.449) [-619.238] (-626.845) (-627.984) -- 0:02:03 685000 -- [-621.493] (-627.167) (-627.783) (-630.671) * (-624.160) (-627.644) [-619.135] (-630.825) -- 0:02:02 Average standard deviation of split frequencies: 0.009090 686000 -- (-625.062) (-623.339) [-626.227] (-636.487) * [-631.885] (-623.856) (-626.376) (-620.811) -- 0:02:02 687000 -- (-621.667) [-627.014] (-629.496) (-633.192) * (-627.486) (-628.135) [-619.415] (-626.241) -- 0:02:02 688000 -- [-623.513] (-630.652) (-629.622) (-628.160) * (-647.819) (-632.854) (-623.210) [-628.774] -- 0:02:01 689000 -- (-625.520) (-628.689) [-622.958] (-633.922) * (-627.307) (-628.830) [-620.466] (-630.676) -- 0:02:01 690000 -- (-625.548) [-616.389] (-619.456) (-632.947) * (-619.528) (-639.738) (-627.725) [-626.969] -- 0:02:00 Average standard deviation of split frequencies: 0.009126 691000 -- [-633.186] (-629.865) (-635.225) (-620.772) * (-622.035) (-630.513) [-617.578] (-622.324) -- 0:02:00 692000 -- (-627.907) (-642.916) (-624.914) [-623.311] * (-623.662) (-631.336) (-625.259) [-624.784] -- 0:02:00 693000 -- [-617.948] (-636.019) (-628.781) (-629.825) * [-624.746] (-631.162) (-624.028) (-632.374) -- 0:01:59 694000 -- [-617.331] (-622.689) (-626.707) (-623.864) * (-633.810) (-630.819) (-623.219) [-624.898] -- 0:01:59 695000 -- (-627.707) [-619.542] (-628.088) (-624.719) * (-631.402) [-628.009] (-627.074) (-627.965) -- 0:01:58 Average standard deviation of split frequencies: 0.008592 696000 -- [-621.988] (-629.226) (-645.159) (-624.446) * (-633.110) (-627.186) [-627.914] (-624.063) -- 0:01:58 697000 -- (-635.213) (-624.332) [-628.943] (-629.284) * (-629.067) (-634.320) [-620.841] (-641.618) -- 0:01:58 698000 -- (-631.983) [-626.338] (-634.109) (-623.583) * (-622.433) [-630.684] (-626.201) (-636.372) -- 0:01:57 699000 -- (-625.392) (-631.213) [-622.513] (-623.334) * (-622.825) (-641.508) (-619.370) [-616.995] -- 0:01:57 700000 -- [-622.040] (-653.736) (-622.401) (-628.752) * (-632.087) (-617.750) [-624.251] (-636.647) -- 0:01:57 Average standard deviation of split frequencies: 0.008593 701000 -- [-618.845] (-648.991) (-632.228) (-623.013) * (-630.104) (-623.685) [-621.802] (-626.243) -- 0:01:56 702000 -- [-619.332] (-642.877) (-629.537) (-635.443) * (-617.049) (-620.083) [-623.070] (-625.202) -- 0:01:56 703000 -- (-626.798) (-623.272) (-650.714) [-621.341] * (-620.545) (-636.262) [-621.634] (-625.567) -- 0:01:55 704000 -- (-631.621) (-623.230) [-627.785] (-637.673) * (-630.178) [-625.653] (-628.765) (-639.229) -- 0:01:55 705000 -- (-627.467) [-621.461] (-636.959) (-629.982) * (-632.969) (-625.008) [-620.251] (-627.118) -- 0:01:55 Average standard deviation of split frequencies: 0.008261 706000 -- (-630.195) [-620.854] (-620.152) (-628.303) * (-641.398) (-644.345) [-627.387] (-621.039) -- 0:01:54 707000 -- (-633.279) (-622.098) [-624.034] (-627.182) * [-614.660] (-640.963) (-630.309) (-645.937) -- 0:01:54 708000 -- (-622.451) [-628.631] (-624.888) (-618.684) * [-622.687] (-621.358) (-622.140) (-629.828) -- 0:01:53 709000 -- [-624.236] (-626.409) (-626.144) (-634.972) * (-628.937) (-625.818) [-612.339] (-634.402) -- 0:01:53 710000 -- (-637.694) (-625.942) (-631.195) [-629.453] * (-634.047) [-623.450] (-626.164) (-617.794) -- 0:01:53 Average standard deviation of split frequencies: 0.008074 711000 -- [-630.361] (-625.706) (-618.804) (-620.004) * (-624.565) (-628.367) (-621.097) [-628.858] -- 0:01:52 712000 -- [-620.002] (-629.133) (-622.976) (-627.472) * (-625.581) (-618.985) [-623.814] (-631.033) -- 0:01:52 713000 -- (-638.558) (-639.734) [-619.428] (-620.724) * (-629.291) [-624.485] (-629.116) (-622.992) -- 0:01:51 714000 -- (-633.382) (-626.020) [-620.733] (-626.265) * (-621.843) [-620.702] (-635.696) (-630.512) -- 0:01:51 715000 -- [-623.522] (-634.646) (-631.791) (-629.291) * (-621.463) [-621.606] (-625.105) (-632.741) -- 0:01:51 Average standard deviation of split frequencies: 0.007844 716000 -- (-619.920) [-629.948] (-618.234) (-625.966) * (-629.651) [-621.235] (-630.554) (-628.122) -- 0:01:50 717000 -- (-620.197) (-629.313) [-623.859] (-627.187) * [-622.647] (-635.965) (-621.072) (-629.478) -- 0:01:50 718000 -- [-619.767] (-623.564) (-620.058) (-624.615) * (-631.422) (-622.228) (-625.203) [-625.580] -- 0:01:49 719000 -- (-627.420) (-622.040) (-621.658) [-616.494] * (-631.158) (-620.716) [-617.157] (-623.600) -- 0:01:49 720000 -- (-629.982) [-623.324] (-630.945) (-635.220) * (-623.126) [-620.481] (-621.037) (-647.854) -- 0:01:49 Average standard deviation of split frequencies: 0.007607 721000 -- (-623.165) [-612.669] (-640.150) (-634.030) * (-623.791) [-620.593] (-635.904) (-622.977) -- 0:01:48 722000 -- (-625.617) (-627.994) (-622.496) [-625.722] * [-624.910] (-627.312) (-629.789) (-627.835) -- 0:01:48 723000 -- (-631.710) (-625.193) (-627.341) [-620.075] * [-625.045] (-629.236) (-631.259) (-622.469) -- 0:01:48 724000 -- [-626.095] (-629.696) (-636.644) (-617.907) * [-631.055] (-620.566) (-619.100) (-624.493) -- 0:01:47 725000 -- (-657.051) [-622.480] (-626.843) (-622.205) * [-621.072] (-626.655) (-633.040) (-625.495) -- 0:01:47 Average standard deviation of split frequencies: 0.007588 726000 -- (-632.404) (-635.438) (-632.648) [-618.980] * [-626.794] (-638.252) (-621.805) (-624.880) -- 0:01:46 727000 -- [-624.432] (-632.151) (-634.857) (-620.088) * (-622.783) [-624.583] (-621.293) (-619.970) -- 0:01:46 728000 -- (-629.949) (-622.899) [-622.055] (-628.433) * [-615.687] (-632.861) (-641.262) (-626.943) -- 0:01:46 729000 -- (-630.172) [-623.782] (-629.865) (-622.804) * (-637.857) (-623.220) (-628.844) [-619.183] -- 0:01:45 730000 -- [-627.965] (-622.546) (-630.257) (-619.862) * (-633.881) [-623.317] (-629.289) (-627.219) -- 0:01:45 Average standard deviation of split frequencies: 0.007502 731000 -- (-621.119) (-624.157) [-617.999] (-630.942) * (-630.295) (-624.963) (-617.279) [-617.075] -- 0:01:44 732000 -- (-623.393) (-629.395) (-624.300) [-620.382] * (-626.932) (-638.435) [-621.105] (-624.161) -- 0:01:44 733000 -- [-627.068] (-628.539) (-628.916) (-630.748) * (-630.065) [-625.982] (-628.970) (-626.029) -- 0:01:44 734000 -- (-631.397) [-627.298] (-627.657) (-623.501) * [-624.458] (-633.408) (-628.221) (-627.523) -- 0:01:43 735000 -- (-635.619) [-631.637] (-633.615) (-636.282) * (-624.468) (-630.715) (-633.696) [-618.996] -- 0:01:43 Average standard deviation of split frequencies: 0.007393 736000 -- (-626.889) (-629.106) (-623.169) [-620.213] * (-624.413) [-628.199] (-625.621) (-626.244) -- 0:01:42 737000 -- (-631.504) [-621.254] (-627.649) (-631.357) * (-624.591) [-620.147] (-625.100) (-624.090) -- 0:01:42 738000 -- (-636.442) [-629.840] (-644.842) (-621.301) * (-620.390) (-616.777) [-622.853] (-627.238) -- 0:01:42 739000 -- (-636.676) [-622.904] (-636.491) (-620.400) * (-617.952) [-628.931] (-627.851) (-628.256) -- 0:01:41 740000 -- (-630.414) [-615.448] (-630.973) (-623.369) * (-627.401) (-628.377) [-614.770] (-625.987) -- 0:01:41 Average standard deviation of split frequencies: 0.007110 741000 -- [-638.390] (-631.285) (-638.081) (-632.964) * (-625.041) [-623.086] (-621.466) (-629.520) -- 0:01:41 742000 -- [-634.532] (-622.131) (-623.067) (-632.950) * (-622.011) [-614.272] (-620.472) (-629.141) -- 0:01:40 743000 -- (-638.695) (-629.171) [-628.566] (-626.910) * (-620.241) (-631.599) (-615.911) [-621.485] -- 0:01:40 744000 -- (-619.207) (-633.194) (-635.612) [-621.990] * [-628.168] (-628.337) (-634.211) (-627.662) -- 0:01:39 745000 -- [-623.437] (-625.755) (-633.298) (-623.238) * [-625.064] (-627.490) (-627.096) (-634.310) -- 0:01:39 Average standard deviation of split frequencies: 0.006969 746000 -- (-619.077) (-631.325) [-618.242] (-622.282) * [-622.952] (-622.312) (-634.187) (-644.947) -- 0:01:39 747000 -- (-630.313) [-622.879] (-623.820) (-627.617) * (-619.028) (-632.582) (-631.600) [-626.417] -- 0:01:38 748000 -- (-625.791) (-627.149) (-631.749) [-622.194] * [-622.175] (-632.448) (-634.867) (-625.025) -- 0:01:38 749000 -- [-615.509] (-623.851) (-626.150) (-619.265) * [-631.957] (-629.730) (-638.543) (-623.155) -- 0:01:37 750000 -- (-634.007) [-622.091] (-631.316) (-640.188) * (-633.919) [-626.740] (-631.387) (-634.827) -- 0:01:37 Average standard deviation of split frequencies: 0.007105 751000 -- (-626.095) (-634.043) [-619.653] (-627.005) * (-630.407) (-640.819) (-616.802) [-621.209] -- 0:01:37 752000 -- (-623.367) (-617.788) [-625.058] (-622.658) * (-633.218) (-624.913) [-623.101] (-624.269) -- 0:01:36 753000 -- (-621.010) [-631.381] (-630.204) (-630.458) * (-639.764) (-633.617) (-634.927) [-612.954] -- 0:01:36 754000 -- (-628.366) [-624.226] (-633.761) (-621.771) * (-630.350) [-625.104] (-630.309) (-624.226) -- 0:01:35 755000 -- (-642.146) [-621.459] (-623.985) (-640.451) * (-626.305) [-622.378] (-634.373) (-629.640) -- 0:01:35 Average standard deviation of split frequencies: 0.007108 756000 -- (-625.592) [-619.129] (-629.095) (-620.619) * (-646.395) [-625.768] (-632.572) (-626.315) -- 0:01:35 757000 -- (-634.075) [-623.879] (-625.977) (-646.403) * (-629.663) (-634.805) [-621.887] (-631.510) -- 0:01:34 758000 -- (-642.469) (-631.002) [-620.995] (-626.607) * [-626.101] (-634.093) (-621.082) (-633.365) -- 0:01:34 759000 -- (-625.613) (-629.833) [-619.268] (-629.263) * [-623.518] (-626.588) (-624.826) (-635.729) -- 0:01:33 760000 -- (-628.500) (-626.733) (-626.840) [-620.204] * [-620.429] (-630.965) (-625.508) (-626.988) -- 0:01:33 Average standard deviation of split frequencies: 0.007313 761000 -- (-622.375) (-627.093) [-623.641] (-621.092) * (-621.176) [-625.656] (-631.237) (-627.779) -- 0:01:33 762000 -- [-620.353] (-634.825) (-625.147) (-628.523) * (-629.826) (-626.176) (-631.952) [-619.211] -- 0:01:32 763000 -- [-625.467] (-631.646) (-625.282) (-639.759) * (-617.661) (-631.203) (-629.462) [-618.571] -- 0:01:32 764000 -- [-627.427] (-629.563) (-627.567) (-638.514) * [-621.310] (-632.910) (-624.395) (-630.840) -- 0:01:32 765000 -- (-628.374) (-627.923) (-622.238) [-619.702] * (-631.520) (-638.731) (-623.576) [-627.423] -- 0:01:31 Average standard deviation of split frequencies: 0.007244 766000 -- (-627.867) [-623.259] (-638.682) (-634.395) * [-621.031] (-643.988) (-622.711) (-632.137) -- 0:01:31 767000 -- (-626.742) [-623.918] (-614.638) (-637.278) * (-619.939) [-622.343] (-621.581) (-630.644) -- 0:01:30 768000 -- (-621.012) [-628.398] (-633.211) (-620.128) * (-628.983) (-619.776) [-616.254] (-622.877) -- 0:01:30 769000 -- (-626.203) (-619.853) [-626.066] (-646.982) * (-626.776) (-635.531) (-636.751) [-620.678] -- 0:01:30 770000 -- (-628.499) (-626.033) (-624.123) [-618.418] * (-621.312) [-628.737] (-624.477) (-629.763) -- 0:01:29 Average standard deviation of split frequencies: 0.007270 771000 -- (-622.964) (-626.029) [-627.387] (-635.375) * (-619.021) (-622.600) (-626.460) [-635.516] -- 0:01:29 772000 -- (-625.416) (-626.300) (-638.867) [-618.572] * (-620.462) (-628.730) [-626.123] (-625.145) -- 0:01:28 773000 -- (-629.711) [-618.769] (-629.243) (-632.986) * (-621.607) (-624.297) [-624.765] (-639.388) -- 0:01:28 774000 -- (-621.515) [-618.042] (-639.867) (-630.164) * [-626.767] (-625.181) (-630.538) (-631.865) -- 0:01:28 775000 -- (-626.524) [-622.998] (-626.431) (-632.935) * (-625.385) (-625.460) (-620.865) [-618.555] -- 0:01:27 Average standard deviation of split frequencies: 0.007134 776000 -- (-630.013) [-626.378] (-615.835) (-619.978) * (-621.158) (-626.472) (-628.807) [-617.258] -- 0:01:27 777000 -- [-618.294] (-631.921) (-621.697) (-636.073) * (-623.617) [-620.625] (-628.064) (-629.518) -- 0:01:26 778000 -- (-630.076) (-622.768) [-622.347] (-623.749) * [-626.471] (-629.713) (-622.768) (-619.327) -- 0:01:26 779000 -- (-616.598) (-632.542) [-622.631] (-634.858) * (-629.719) [-623.369] (-622.644) (-622.698) -- 0:01:26 780000 -- (-621.211) (-626.024) (-632.288) [-626.321] * (-617.376) [-618.721] (-625.212) (-627.978) -- 0:01:25 Average standard deviation of split frequencies: 0.006591 781000 -- (-628.936) (-635.891) (-621.459) [-625.938] * (-624.813) [-616.111] (-629.690) (-629.389) -- 0:01:25 782000 -- (-638.708) (-628.732) (-625.893) [-625.193] * (-628.683) [-634.961] (-614.869) (-628.343) -- 0:01:25 783000 -- (-624.354) [-630.159] (-625.823) (-631.921) * (-623.785) [-624.189] (-621.629) (-626.180) -- 0:01:24 784000 -- [-620.721] (-627.275) (-629.970) (-632.054) * (-629.302) (-640.342) (-622.110) [-630.041] -- 0:01:24 785000 -- [-620.605] (-642.698) (-617.976) (-613.019) * [-627.029] (-633.974) (-616.272) (-634.600) -- 0:01:23 Average standard deviation of split frequencies: 0.006632 786000 -- (-619.617) [-629.741] (-623.571) (-637.700) * (-621.312) [-624.847] (-624.569) (-628.800) -- 0:01:23 787000 -- (-626.210) (-639.246) [-626.213] (-629.128) * (-619.878) (-623.541) [-624.053] (-628.709) -- 0:01:23 788000 -- (-626.488) [-625.638] (-622.920) (-629.920) * [-618.268] (-621.172) (-630.745) (-634.897) -- 0:01:22 789000 -- (-629.972) (-633.764) (-626.640) [-626.847] * [-625.053] (-632.125) (-624.836) (-623.027) -- 0:01:22 790000 -- (-617.194) (-640.190) [-624.186] (-628.531) * [-625.474] (-626.261) (-620.107) (-621.562) -- 0:01:21 Average standard deviation of split frequencies: 0.006609 791000 -- (-617.054) [-628.462] (-630.032) (-633.370) * (-639.882) (-628.396) (-625.278) [-613.228] -- 0:01:21 792000 -- [-616.165] (-624.101) (-616.276) (-635.907) * [-631.116] (-623.759) (-623.423) (-629.722) -- 0:01:21 793000 -- (-621.343) [-623.671] (-626.647) (-625.637) * (-624.152) (-625.735) [-630.096] (-620.212) -- 0:01:20 794000 -- (-627.090) [-625.936] (-627.354) (-631.908) * (-625.451) (-622.299) [-613.766] (-631.715) -- 0:01:20 795000 -- (-634.721) [-627.675] (-620.964) (-618.744) * (-627.595) (-625.385) [-626.034] (-640.273) -- 0:01:19 Average standard deviation of split frequencies: 0.006650 796000 -- [-623.537] (-652.026) (-625.754) (-619.993) * [-629.710] (-639.074) (-629.623) (-632.203) -- 0:01:19 797000 -- [-617.863] (-636.029) (-626.370) (-622.326) * (-622.474) (-618.183) [-619.057] (-630.650) -- 0:01:19 798000 -- [-621.715] (-634.373) (-620.667) (-621.349) * (-630.498) [-628.001] (-628.102) (-622.108) -- 0:01:18 799000 -- (-627.947) (-625.735) [-616.948] (-623.810) * [-625.968] (-625.676) (-623.711) (-619.151) -- 0:01:18 800000 -- (-631.121) [-618.826] (-628.896) (-624.223) * (-627.289) [-622.383] (-618.251) (-623.986) -- 0:01:18 Average standard deviation of split frequencies: 0.006544 801000 -- (-630.993) [-623.086] (-625.296) (-624.321) * (-626.505) [-622.083] (-634.064) (-627.626) -- 0:01:17 802000 -- [-615.024] (-627.220) (-618.798) (-631.930) * [-631.303] (-631.606) (-614.329) (-625.687) -- 0:01:17 803000 -- (-623.476) [-628.233] (-636.278) (-620.222) * (-635.729) [-620.337] (-635.364) (-617.477) -- 0:01:16 804000 -- (-630.509) [-626.210] (-627.946) (-622.268) * (-630.454) (-626.958) [-624.733] (-638.963) -- 0:01:16 805000 -- (-627.561) [-619.427] (-627.799) (-619.854) * (-627.678) (-620.291) (-636.523) [-625.842] -- 0:01:16 Average standard deviation of split frequencies: 0.006584 806000 -- (-635.977) (-627.067) [-631.564] (-622.612) * (-626.729) (-629.041) [-625.605] (-632.641) -- 0:01:15 807000 -- (-621.550) (-618.733) [-622.291] (-626.413) * (-633.996) (-625.070) [-624.901] (-626.484) -- 0:01:15 808000 -- (-623.867) [-618.332] (-634.105) (-629.666) * (-631.396) (-634.690) (-632.359) [-618.066] -- 0:01:14 809000 -- (-622.904) (-627.584) (-621.733) [-637.217] * [-615.129] (-622.289) (-624.956) (-625.544) -- 0:01:14 810000 -- (-632.589) (-628.788) (-621.201) [-626.833] * (-627.891) (-616.680) [-619.797] (-626.703) -- 0:01:14 Average standard deviation of split frequencies: 0.006779 811000 -- (-629.455) (-625.562) (-633.357) [-619.666] * (-633.056) [-620.480] (-630.039) (-623.440) -- 0:01:13 812000 -- [-627.332] (-620.683) (-629.533) (-635.700) * [-623.761] (-627.963) (-628.116) (-636.116) -- 0:01:13 813000 -- (-632.617) [-618.248] (-622.340) (-620.520) * [-617.243] (-624.759) (-629.965) (-640.000) -- 0:01:12 814000 -- [-617.677] (-628.829) (-629.919) (-624.376) * (-621.001) [-622.151] (-629.052) (-628.981) -- 0:01:12 815000 -- (-635.089) [-623.441] (-635.701) (-624.659) * (-619.359) (-619.069) (-632.473) [-620.668] -- 0:01:12 Average standard deviation of split frequencies: 0.006916 816000 -- (-637.464) [-621.713] (-621.959) (-630.019) * (-617.186) [-625.318] (-633.512) (-628.253) -- 0:01:11 817000 -- (-626.268) [-622.388] (-642.217) (-626.199) * (-627.912) (-636.510) (-621.435) [-625.145] -- 0:01:11 818000 -- (-638.066) [-631.946] (-623.028) (-627.999) * (-622.061) [-623.065] (-637.144) (-626.139) -- 0:01:10 819000 -- (-622.703) (-625.300) (-631.692) [-625.751] * (-632.257) [-617.141] (-625.995) (-619.692) -- 0:01:10 820000 -- (-621.937) [-626.581] (-628.770) (-623.660) * (-632.487) (-639.785) (-632.413) [-613.746] -- 0:01:10 Average standard deviation of split frequencies: 0.007106 821000 -- [-633.749] (-629.864) (-625.071) (-621.807) * (-622.347) (-634.481) [-623.499] (-625.719) -- 0:01:09 822000 -- (-635.828) (-630.566) (-623.819) [-630.222] * [-624.549] (-627.561) (-635.924) (-623.725) -- 0:01:09 823000 -- [-623.148] (-627.870) (-633.985) (-629.479) * (-626.711) (-627.406) [-613.600] (-618.854) -- 0:01:09 824000 -- (-623.099) [-624.801] (-633.080) (-632.674) * (-631.872) [-627.796] (-625.534) (-626.488) -- 0:01:08 825000 -- (-619.963) (-622.967) [-632.441] (-631.051) * [-634.182] (-631.551) (-633.951) (-632.652) -- 0:01:08 Average standard deviation of split frequencies: 0.006881 826000 -- [-617.218] (-624.162) (-625.718) (-626.702) * [-623.166] (-622.536) (-635.517) (-626.789) -- 0:01:07 827000 -- (-623.097) (-631.854) (-631.045) [-623.399] * [-624.684] (-638.634) (-623.468) (-626.405) -- 0:01:07 828000 -- (-624.419) (-634.956) (-626.698) [-620.693] * (-620.139) (-630.337) [-616.385] (-624.272) -- 0:01:07 829000 -- [-623.849] (-628.266) (-621.998) (-633.047) * (-621.242) (-626.715) [-617.254] (-618.995) -- 0:01:06 830000 -- (-621.770) (-630.838) (-625.402) [-621.506] * (-623.741) (-635.354) [-622.229] (-617.785) -- 0:01:06 Average standard deviation of split frequencies: 0.006891 831000 -- (-630.813) (-621.678) [-620.236] (-639.171) * (-629.988) (-618.142) (-620.199) [-619.989] -- 0:01:05 832000 -- (-643.984) (-626.671) [-626.843] (-620.467) * (-630.921) [-615.728] (-621.780) (-636.224) -- 0:01:05 833000 -- (-617.767) (-629.545) (-645.205) [-622.402] * (-634.661) (-622.560) (-639.767) [-628.069] -- 0:01:05 834000 -- (-625.041) [-623.837] (-629.307) (-638.293) * (-625.138) (-622.530) (-630.077) [-623.976] -- 0:01:04 835000 -- [-620.439] (-623.796) (-629.061) (-629.059) * (-631.828) (-635.464) [-623.634] (-624.348) -- 0:01:04 Average standard deviation of split frequencies: 0.006912 836000 -- (-624.882) (-635.125) (-620.568) [-625.934] * [-622.373] (-624.367) (-623.792) (-628.957) -- 0:01:03 837000 -- (-631.846) [-630.323] (-620.707) (-628.824) * (-636.354) (-619.856) (-614.972) [-620.396] -- 0:01:03 838000 -- [-631.617] (-623.945) (-622.590) (-625.886) * [-619.341] (-635.344) (-626.459) (-634.008) -- 0:01:03 839000 -- (-624.523) (-621.323) [-614.015] (-623.441) * (-630.810) (-628.408) (-621.685) [-624.438] -- 0:01:02 840000 -- (-632.606) (-626.577) (-623.172) [-627.655] * [-622.650] (-623.982) (-627.822) (-626.327) -- 0:01:02 Average standard deviation of split frequencies: 0.006761 841000 -- (-630.147) (-623.538) (-623.080) [-621.116] * [-621.964] (-625.567) (-626.895) (-627.101) -- 0:01:02 842000 -- (-625.745) (-628.494) [-622.448] (-615.460) * (-627.140) (-627.099) [-624.666] (-627.879) -- 0:01:01 843000 -- (-626.588) [-620.856] (-624.723) (-621.026) * (-627.604) (-625.180) (-616.932) [-618.825] -- 0:01:01 844000 -- (-628.244) (-629.081) (-626.382) [-621.332] * (-628.436) (-632.571) [-626.938] (-630.267) -- 0:01:00 845000 -- (-627.408) (-635.650) (-620.227) [-624.582] * (-619.728) [-623.528] (-625.077) (-635.201) -- 0:01:00 Average standard deviation of split frequencies: 0.006671 846000 -- [-628.796] (-635.514) (-627.091) (-618.633) * (-629.723) [-619.577] (-623.835) (-632.226) -- 0:01:00 847000 -- (-625.906) (-624.070) [-629.075] (-631.497) * (-623.684) (-631.567) [-621.410] (-625.686) -- 0:00:59 848000 -- (-620.470) [-630.710] (-629.484) (-624.840) * [-618.554] (-636.279) (-619.497) (-626.506) -- 0:00:59 849000 -- (-634.368) [-619.531] (-624.424) (-623.133) * (-617.478) [-626.552] (-622.144) (-627.279) -- 0:00:58 850000 -- (-620.425) (-641.597) (-629.669) [-623.514] * (-634.475) [-620.586] (-616.702) (-639.847) -- 0:00:58 Average standard deviation of split frequencies: 0.006523 851000 -- (-625.404) (-632.652) (-622.536) [-617.317] * (-629.575) (-626.518) [-630.293] (-632.448) -- 0:00:58 852000 -- (-618.273) (-620.832) [-625.976] (-628.145) * (-631.067) (-623.196) (-617.871) [-624.670] -- 0:00:57 853000 -- (-620.620) (-632.699) [-617.700] (-644.532) * (-637.157) [-619.597] (-618.264) (-629.675) -- 0:00:57 854000 -- (-626.301) (-627.278) [-628.528] (-638.280) * (-626.290) [-620.900] (-633.102) (-637.412) -- 0:00:56 855000 -- (-633.728) (-631.352) (-620.110) [-623.989] * (-624.373) (-627.159) [-632.043] (-626.984) -- 0:00:56 Average standard deviation of split frequencies: 0.006546 856000 -- (-625.744) [-621.020] (-622.833) (-631.962) * (-625.556) [-625.336] (-626.850) (-629.395) -- 0:00:56 857000 -- (-642.555) [-619.986] (-638.901) (-634.279) * (-624.599) (-630.802) (-618.958) [-625.147] -- 0:00:55 858000 -- (-628.619) (-623.656) [-625.018] (-634.856) * [-618.473] (-640.867) (-622.058) (-625.737) -- 0:00:55 859000 -- (-626.564) (-633.796) (-633.055) [-627.845] * (-629.060) (-635.673) (-624.196) [-623.104] -- 0:00:54 860000 -- (-619.408) (-625.820) (-636.990) [-621.823] * (-631.327) (-626.533) (-627.165) [-619.496] -- 0:00:54 Average standard deviation of split frequencies: 0.006588 861000 -- [-620.367] (-640.801) (-631.278) (-640.402) * (-631.328) [-625.132] (-621.831) (-636.203) -- 0:00:54 862000 -- (-622.119) (-628.784) (-633.168) [-621.610] * (-623.924) (-636.705) [-616.002] (-629.966) -- 0:00:53 863000 -- [-621.199] (-627.438) (-624.441) (-622.359) * [-616.947] (-624.252) (-624.032) (-632.481) -- 0:00:53 864000 -- (-628.544) [-618.503] (-637.960) (-630.562) * [-626.653] (-632.832) (-627.519) (-625.402) -- 0:00:53 865000 -- (-632.462) (-624.471) [-617.858] (-628.636) * (-616.963) (-640.152) (-626.527) [-623.991] -- 0:00:52 Average standard deviation of split frequencies: 0.006610 866000 -- (-622.595) (-633.084) [-630.137] (-640.039) * (-625.189) [-620.996] (-619.566) (-619.484) -- 0:00:52 867000 -- (-628.402) (-636.226) (-619.692) [-618.784] * (-620.220) [-621.285] (-634.079) (-626.590) -- 0:00:51 868000 -- (-624.957) (-629.274) (-643.318) [-621.784] * [-620.704] (-619.727) (-634.105) (-640.539) -- 0:00:51 869000 -- (-636.965) (-628.445) (-618.177) [-621.867] * (-627.755) (-626.678) [-626.465] (-635.791) -- 0:00:51 870000 -- (-629.685) (-641.844) (-626.695) [-619.230] * (-627.354) [-624.059] (-620.904) (-630.332) -- 0:00:50 Average standard deviation of split frequencies: 0.006760 871000 -- (-618.218) [-630.165] (-638.649) (-618.278) * (-631.145) (-630.490) (-629.264) [-626.043] -- 0:00:50 872000 -- (-622.824) [-618.165] (-628.739) (-623.070) * (-623.488) (-619.989) (-619.381) [-618.763] -- 0:00:49 873000 -- [-620.228] (-621.956) (-629.607) (-630.767) * [-623.316] (-629.553) (-624.214) (-628.685) -- 0:00:49 874000 -- [-628.006] (-625.611) (-629.335) (-626.347) * [-630.895] (-631.449) (-622.253) (-630.166) -- 0:00:49 875000 -- (-623.793) [-619.566] (-624.289) (-628.057) * [-630.779] (-615.268) (-627.128) (-628.021) -- 0:00:48 Average standard deviation of split frequencies: 0.006381 876000 -- (-622.040) (-635.735) (-622.647) [-628.570] * [-625.127] (-620.195) (-634.220) (-640.683) -- 0:00:48 877000 -- (-630.189) (-617.619) [-624.442] (-630.497) * (-628.357) [-621.584] (-632.608) (-620.109) -- 0:00:47 878000 -- (-635.375) [-624.499] (-619.418) (-628.566) * (-626.761) (-624.003) (-626.996) [-618.808] -- 0:00:47 879000 -- [-618.998] (-627.879) (-638.532) (-621.092) * (-620.270) (-627.629) (-625.756) [-626.500] -- 0:00:47 880000 -- (-620.561) [-626.353] (-620.932) (-626.387) * [-620.633] (-619.264) (-624.922) (-624.305) -- 0:00:46 Average standard deviation of split frequencies: 0.006316 881000 -- [-625.491] (-638.382) (-621.267) (-625.068) * (-628.117) (-628.469) (-621.231) [-621.381] -- 0:00:46 882000 -- [-621.423] (-620.343) (-633.632) (-628.770) * (-626.915) [-620.572] (-630.856) (-646.229) -- 0:00:46 883000 -- (-627.091) (-616.832) (-622.616) [-617.853] * [-627.565] (-623.928) (-622.602) (-636.584) -- 0:00:45 884000 -- (-629.534) [-624.505] (-625.915) (-615.228) * (-627.635) [-623.507] (-623.150) (-628.858) -- 0:00:45 885000 -- [-616.968] (-627.444) (-621.473) (-631.611) * [-622.274] (-631.052) (-637.850) (-630.980) -- 0:00:44 Average standard deviation of split frequencies: 0.006461 886000 -- (-624.261) (-634.844) [-627.412] (-634.005) * (-627.200) (-629.636) [-616.676] (-626.915) -- 0:00:44 887000 -- [-619.331] (-640.143) (-631.664) (-626.688) * (-629.248) (-628.726) [-622.945] (-623.141) -- 0:00:44 888000 -- (-628.360) (-635.947) (-626.898) [-620.719] * (-629.066) [-627.463] (-621.649) (-643.784) -- 0:00:43 889000 -- [-628.490] (-630.250) (-617.914) (-628.445) * [-617.105] (-624.326) (-619.835) (-618.886) -- 0:00:43 890000 -- (-619.602) [-621.647] (-618.689) (-636.343) * (-622.533) (-623.166) [-621.290] (-619.809) -- 0:00:42 Average standard deviation of split frequencies: 0.006518 891000 -- [-619.088] (-625.190) (-623.694) (-632.596) * [-625.478] (-617.914) (-634.617) (-628.091) -- 0:00:42 892000 -- (-630.018) (-634.506) [-636.431] (-626.421) * (-630.379) [-616.105] (-622.087) (-627.007) -- 0:00:42 893000 -- (-627.009) (-624.166) [-625.682] (-626.979) * (-626.889) (-618.690) [-622.294] (-628.261) -- 0:00:41 894000 -- (-628.318) [-620.350] (-632.613) (-628.601) * (-620.145) [-617.352] (-630.296) (-631.145) -- 0:00:41 895000 -- (-625.990) (-624.580) (-630.912) [-627.983] * (-620.650) (-630.808) (-630.072) [-625.248] -- 0:00:40 Average standard deviation of split frequencies: 0.006494 896000 -- (-623.161) (-626.208) [-619.724] (-630.090) * (-634.516) (-626.492) (-619.720) [-622.843] -- 0:00:40 897000 -- (-620.559) (-624.065) [-627.815] (-625.146) * (-626.354) (-631.939) [-626.684] (-630.008) -- 0:00:40 898000 -- (-620.422) (-628.690) (-625.587) [-631.546] * (-637.853) (-632.159) [-636.324] (-624.618) -- 0:00:39 899000 -- [-616.671] (-636.195) (-624.979) (-629.446) * (-631.651) (-626.514) [-623.952] (-625.956) -- 0:00:39 900000 -- [-626.824] (-628.055) (-625.878) (-625.347) * (-623.233) (-633.289) [-625.214] (-631.948) -- 0:00:39 Average standard deviation of split frequencies: 0.006445 901000 -- (-630.489) (-624.211) [-621.016] (-635.456) * (-624.469) (-624.626) [-630.054] (-623.928) -- 0:00:38 902000 -- [-621.533] (-626.780) (-627.406) (-634.878) * (-620.741) (-633.653) (-624.232) [-626.949] -- 0:00:38 903000 -- [-614.697] (-631.328) (-631.759) (-625.887) * (-633.635) (-623.992) [-618.106] (-623.196) -- 0:00:37 904000 -- (-627.445) (-627.562) (-626.428) [-623.443] * (-628.928) (-628.739) (-629.635) [-622.557] -- 0:00:37 905000 -- (-618.957) (-634.642) (-629.769) [-620.917] * (-634.242) (-627.328) (-619.817) [-626.973] -- 0:00:37 Average standard deviation of split frequencies: 0.006938 906000 -- (-620.427) (-625.508) (-617.661) [-630.223] * (-621.347) (-634.812) (-627.712) [-629.301] -- 0:00:36 907000 -- (-633.519) (-626.331) [-620.588] (-634.793) * (-623.898) (-628.175) (-625.305) [-617.473] -- 0:00:36 908000 -- (-625.863) (-621.529) [-624.691] (-630.385) * (-620.633) (-633.195) (-619.586) [-621.059] -- 0:00:35 909000 -- (-624.283) (-631.456) (-620.359) [-624.946] * (-631.031) [-618.895] (-628.386) (-615.283) -- 0:00:35 910000 -- (-623.645) (-635.194) (-617.509) [-619.989] * (-635.024) (-622.597) [-634.842] (-624.197) -- 0:00:35 Average standard deviation of split frequencies: 0.006537 911000 -- (-621.352) (-624.315) [-616.980] (-630.307) * (-624.064) (-624.847) (-625.675) [-614.612] -- 0:00:34 912000 -- (-630.451) [-621.129] (-628.079) (-628.211) * (-637.795) (-626.914) [-622.372] (-627.260) -- 0:00:34 913000 -- (-631.620) [-630.273] (-619.671) (-622.112) * (-648.559) [-618.093] (-625.484) (-636.786) -- 0:00:33 914000 -- (-629.561) [-626.993] (-638.634) (-634.106) * (-633.830) (-615.797) [-629.913] (-625.205) -- 0:00:33 915000 -- [-624.249] (-625.826) (-638.784) (-626.600) * (-627.059) (-623.454) [-624.338] (-630.318) -- 0:00:33 Average standard deviation of split frequencies: 0.006293 916000 -- (-637.699) (-628.784) [-625.217] (-632.472) * (-631.376) (-621.992) [-620.125] (-630.436) -- 0:00:32 917000 -- (-623.855) [-624.785] (-626.324) (-627.161) * (-624.321) [-624.337] (-627.533) (-629.845) -- 0:00:32 918000 -- (-636.872) (-639.877) (-632.944) [-625.777] * (-628.569) (-628.654) (-631.372) [-625.377] -- 0:00:31 919000 -- (-638.346) [-619.740] (-633.307) (-626.585) * (-622.340) (-639.722) [-616.962] (-626.085) -- 0:00:31 920000 -- (-630.375) [-625.910] (-628.958) (-639.726) * [-623.242] (-636.550) (-630.461) (-620.818) -- 0:00:31 Average standard deviation of split frequencies: 0.006481 921000 -- (-628.474) (-637.063) [-628.138] (-624.731) * (-619.241) (-648.174) (-627.959) [-620.809] -- 0:00:30 922000 -- (-631.013) [-624.337] (-625.568) (-626.939) * (-632.729) (-640.284) (-629.515) [-629.696] -- 0:00:30 923000 -- (-626.436) (-651.086) (-619.632) [-626.953] * [-623.401] (-630.371) (-642.659) (-625.860) -- 0:00:30 924000 -- (-621.829) (-633.915) (-625.615) [-619.866] * [-621.354] (-639.615) (-627.295) (-625.196) -- 0:00:29 925000 -- [-629.329] (-634.026) (-626.870) (-628.675) * (-634.761) (-631.001) [-621.166] (-629.353) -- 0:00:29 Average standard deviation of split frequencies: 0.006531 926000 -- [-622.151] (-621.381) (-625.566) (-639.717) * (-636.646) (-634.311) (-627.521) [-617.044] -- 0:00:28 927000 -- (-626.507) [-623.965] (-630.344) (-629.427) * (-619.676) (-631.922) (-618.156) [-622.277] -- 0:00:28 928000 -- (-619.268) (-636.649) [-618.327] (-624.458) * [-621.581] (-623.516) (-615.408) (-631.106) -- 0:00:28 929000 -- [-626.146] (-619.691) (-627.247) (-624.523) * (-629.141) [-620.319] (-629.095) (-622.052) -- 0:00:27 930000 -- (-634.340) (-622.320) (-629.553) [-620.931] * [-623.035] (-618.136) (-625.442) (-620.458) -- 0:00:27 Average standard deviation of split frequencies: 0.006585 931000 -- (-638.456) (-624.642) (-629.913) [-621.615] * [-620.602] (-623.953) (-631.981) (-627.969) -- 0:00:26 932000 -- (-629.217) [-620.273] (-643.162) (-624.288) * (-619.781) (-624.393) [-623.684] (-639.168) -- 0:00:26 933000 -- (-635.403) [-627.388] (-629.512) (-630.686) * (-634.265) (-634.423) [-624.293] (-628.346) -- 0:00:26 934000 -- (-622.807) (-637.139) [-622.748] (-635.953) * (-616.722) (-642.232) [-626.029] (-625.970) -- 0:00:25 935000 -- [-626.231] (-624.890) (-635.660) (-629.343) * (-623.203) (-625.990) [-616.194] (-630.669) -- 0:00:25 Average standard deviation of split frequencies: 0.007037 936000 -- (-635.072) [-628.308] (-630.763) (-639.885) * (-633.355) (-628.234) (-624.013) [-622.903] -- 0:00:24 937000 -- (-623.447) (-637.260) (-622.648) [-622.341] * (-634.819) [-620.211] (-626.788) (-630.027) -- 0:00:24 938000 -- (-630.043) [-629.840] (-626.854) (-634.187) * (-628.918) [-617.632] (-631.691) (-619.432) -- 0:00:24 939000 -- (-628.090) (-640.615) (-620.504) [-625.656] * (-622.418) (-636.416) (-624.832) [-621.580] -- 0:00:23 940000 -- (-629.101) (-643.695) (-625.880) [-616.778] * (-625.113) [-617.211] (-623.312) (-623.231) -- 0:00:23 Average standard deviation of split frequencies: 0.006329 941000 -- (-620.878) (-630.517) [-629.337] (-622.854) * (-630.059) (-629.930) (-624.241) [-621.729] -- 0:00:23 942000 -- [-630.313] (-626.853) (-641.864) (-619.617) * (-624.820) [-620.602] (-626.743) (-621.221) -- 0:00:22 943000 -- (-629.587) (-620.706) (-628.077) [-620.150] * [-617.809] (-642.141) (-632.130) (-630.220) -- 0:00:22 944000 -- (-627.610) (-623.354) (-630.475) [-627.408] * (-631.629) (-628.924) [-619.514] (-625.350) -- 0:00:21 945000 -- (-625.631) (-624.088) (-623.043) [-626.252] * [-624.587] (-620.650) (-631.399) (-628.249) -- 0:00:21 Average standard deviation of split frequencies: 0.006393 946000 -- (-633.843) [-622.325] (-624.499) (-621.649) * (-626.050) (-630.064) [-619.654] (-626.641) -- 0:00:21 947000 -- (-638.127) [-636.803] (-625.252) (-627.430) * (-634.427) (-631.817) [-618.679] (-621.894) -- 0:00:20 948000 -- (-643.078) (-621.351) [-628.053] (-631.889) * (-624.458) (-624.241) (-625.036) [-626.084] -- 0:00:20 949000 -- (-629.598) (-630.687) (-621.286) [-622.789] * (-632.803) [-621.977] (-629.256) (-630.376) -- 0:00:19 950000 -- [-616.831] (-620.670) (-626.675) (-625.066) * (-636.815) [-616.894] (-627.946) (-618.271) -- 0:00:19 Average standard deviation of split frequencies: 0.006361 951000 -- (-632.913) (-630.949) (-625.753) [-621.085] * (-625.890) [-619.376] (-630.415) (-621.826) -- 0:00:19 952000 -- (-632.619) [-629.182] (-628.818) (-629.674) * (-631.024) (-647.475) (-624.445) [-618.236] -- 0:00:18 953000 -- (-629.838) [-621.303] (-627.594) (-624.037) * (-632.092) [-619.112] (-632.936) (-614.094) -- 0:00:18 954000 -- (-620.289) (-645.157) [-621.139] (-625.051) * [-617.041] (-623.669) (-627.573) (-630.411) -- 0:00:17 955000 -- [-628.249] (-614.917) (-622.700) (-625.280) * (-621.994) (-622.613) [-629.866] (-622.670) -- 0:00:17 Average standard deviation of split frequencies: 0.006467 956000 -- (-627.377) [-618.161] (-624.029) (-628.936) * (-624.435) [-631.018] (-630.416) (-619.338) -- 0:00:17 957000 -- [-630.045] (-622.758) (-624.547) (-621.901) * (-632.308) [-624.611] (-621.916) (-628.172) -- 0:00:16 958000 -- (-630.194) (-624.874) (-617.228) [-616.912] * (-634.608) [-625.503] (-619.021) (-622.735) -- 0:00:16 959000 -- (-618.153) (-629.226) [-621.192] (-626.759) * (-629.346) (-628.128) (-635.867) [-621.688] -- 0:00:15 960000 -- (-629.264) (-627.061) [-623.730] (-628.639) * (-614.288) [-629.403] (-626.878) (-618.034) -- 0:00:15 Average standard deviation of split frequencies: 0.006463 961000 -- (-631.572) [-625.998] (-622.771) (-623.565) * (-627.038) [-621.819] (-617.038) (-621.720) -- 0:00:15 962000 -- (-628.523) [-614.327] (-621.333) (-621.876) * (-623.224) (-622.159) [-616.491] (-627.403) -- 0:00:14 963000 -- (-628.563) (-633.265) [-623.158] (-628.504) * (-620.168) (-627.627) (-621.624) [-617.320] -- 0:00:14 964000 -- (-622.236) [-618.115] (-625.760) (-632.524) * (-623.337) (-630.591) [-623.033] (-633.130) -- 0:00:14 965000 -- (-627.608) (-634.154) [-624.419] (-627.434) * (-631.140) [-626.811] (-619.340) (-621.943) -- 0:00:13 Average standard deviation of split frequencies: 0.006539 966000 -- (-636.166) [-633.437] (-627.372) (-625.905) * (-627.754) (-622.506) [-619.414] (-629.043) -- 0:00:13 967000 -- (-634.673) (-627.693) [-632.625] (-624.850) * (-635.197) (-628.393) (-626.925) [-620.408] -- 0:00:12 968000 -- (-624.437) (-626.898) [-621.114] (-619.242) * (-635.683) (-628.678) (-621.734) [-622.120] -- 0:00:12 969000 -- (-638.780) [-627.757] (-631.375) (-622.576) * (-626.160) (-639.425) [-628.332] (-635.084) -- 0:00:12 970000 -- (-634.789) (-634.265) (-630.839) [-623.693] * (-621.507) (-628.069) [-620.434] (-621.029) -- 0:00:11 Average standard deviation of split frequencies: 0.006535 971000 -- (-630.729) (-622.796) (-616.087) [-624.122] * [-627.273] (-636.165) (-638.383) (-636.159) -- 0:00:11 972000 -- (-638.861) (-626.948) [-628.589] (-633.491) * (-631.393) [-625.596] (-630.130) (-618.968) -- 0:00:10 973000 -- (-632.887) (-627.062) (-623.502) [-625.879] * (-635.553) (-635.278) [-627.601] (-623.667) -- 0:00:10 974000 -- (-619.337) [-621.101] (-624.944) (-626.205) * [-617.829] (-614.864) (-631.068) (-623.950) -- 0:00:10 975000 -- (-637.390) (-631.294) (-647.311) [-613.400] * (-623.072) [-619.516] (-628.421) (-630.311) -- 0:00:09 Average standard deviation of split frequencies: 0.006431 976000 -- [-615.500] (-624.145) (-628.138) (-624.259) * [-627.528] (-620.491) (-632.093) (-626.704) -- 0:00:09 977000 -- [-613.478] (-631.212) (-630.219) (-622.804) * [-632.547] (-631.241) (-630.731) (-625.485) -- 0:00:08 978000 -- [-617.156] (-627.117) (-631.788) (-625.118) * [-634.341] (-633.428) (-624.476) (-625.473) -- 0:00:08 979000 -- (-634.156) (-620.796) (-631.803) [-623.579] * (-613.032) [-619.055] (-622.442) (-629.334) -- 0:00:08 980000 -- (-628.097) [-620.339] (-623.690) (-624.757) * (-627.896) (-630.691) (-629.040) [-617.730] -- 0:00:07 Average standard deviation of split frequencies: 0.006414 981000 -- (-632.572) (-626.618) (-627.149) [-617.683] * (-626.023) [-621.739] (-622.786) (-624.483) -- 0:00:07 982000 -- (-631.906) (-626.560) (-629.734) [-631.903] * (-631.250) [-622.410] (-628.807) (-635.528) -- 0:00:07 983000 -- (-626.873) (-627.483) [-626.809] (-638.881) * (-624.861) (-626.134) (-625.931) [-621.026] -- 0:00:06 984000 -- [-620.132] (-624.812) (-641.776) (-624.196) * (-633.403) (-622.533) [-623.377] (-626.238) -- 0:00:06 985000 -- (-627.779) (-627.608) [-614.699] (-633.483) * (-616.410) (-625.662) (-631.504) [-620.627] -- 0:00:05 Average standard deviation of split frequencies: 0.006325 986000 -- (-631.091) (-619.883) [-619.882] (-627.790) * (-616.941) (-628.225) (-622.501) [-623.519] -- 0:00:05 987000 -- (-631.033) [-629.969] (-619.696) (-621.728) * (-634.399) [-622.589] (-618.552) (-619.082) -- 0:00:05 988000 -- (-616.228) [-626.679] (-625.789) (-629.678) * [-626.485] (-627.554) (-626.091) (-626.142) -- 0:00:04 989000 -- (-629.412) (-633.571) (-635.420) [-628.789] * [-618.436] (-628.997) (-633.008) (-627.915) -- 0:00:04 990000 -- (-632.794) (-624.565) [-621.986] (-624.197) * [-620.967] (-632.757) (-629.945) (-619.986) -- 0:00:03 Average standard deviation of split frequencies: 0.006458 991000 -- (-624.586) (-624.825) [-626.330] (-629.685) * [-619.186] (-627.229) (-627.395) (-632.250) -- 0:00:03 992000 -- (-622.523) (-625.566) (-635.359) [-627.821] * [-625.274] (-633.307) (-630.734) (-620.484) -- 0:00:03 993000 -- (-621.860) [-623.225] (-625.811) (-631.228) * (-630.645) (-629.133) [-622.466] (-630.301) -- 0:00:02 994000 -- [-623.193] (-636.348) (-623.295) (-641.905) * (-624.611) (-636.101) [-622.084] (-620.856) -- 0:00:02 995000 -- (-622.654) (-624.376) [-629.383] (-630.252) * (-624.693) [-621.780] (-635.168) (-626.290) -- 0:00:01 Average standard deviation of split frequencies: 0.006518 996000 -- (-618.857) (-633.465) [-617.939] (-642.295) * [-628.359] (-629.655) (-633.473) (-624.934) -- 0:00:01 997000 -- (-626.856) (-629.693) [-626.821] (-626.151) * [-622.607] (-619.789) (-632.520) (-626.546) -- 0:00:01 998000 -- (-622.775) (-622.175) [-622.317] (-630.069) * [-616.560] (-630.356) (-625.928) (-639.398) -- 0:00:00 999000 -- (-627.122) [-621.357] (-628.811) (-626.439) * (-622.526) (-622.610) (-635.039) [-622.261] -- 0:00:00 1000000 -- (-617.953) [-626.209] (-629.519) (-636.161) * (-625.082) [-628.998] (-624.841) (-631.637) -- 0:00:00 Average standard deviation of split frequencies: 0.006138 Analysis completed in 6 mins 31 seconds Analysis used 390.89 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -609.67 Likelihood of best state for "cold" chain of run 2 was -609.82 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 66.2 % ( 55 %) Dirichlet(Revmat{all}) 79.6 % ( 68 %) Slider(Revmat{all}) 40.6 % ( 33 %) Dirichlet(Pi{all}) 39.7 % ( 27 %) Slider(Pi{all}) 73.9 % ( 57 %) Multiplier(Alpha{1,2}) 71.5 % ( 51 %) Multiplier(Alpha{3}) 89.2 % ( 78 %) Slider(Pinvar{all}) 43.7 % ( 41 %) ExtSPR(Tau{all},V{all}) 31.2 % ( 31 %) ExtTBR(Tau{all},V{all}) 47.0 % ( 40 %) NNI(Tau{all},V{all}) 37.9 % ( 32 %) ParsSPR(Tau{all},V{all}) 27.4 % ( 29 %) Multiplier(V{all}) 67.5 % ( 68 %) Nodeslider(V{all}) 26.3 % ( 30 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 66.4 % ( 56 %) Dirichlet(Revmat{all}) 80.3 % ( 79 %) Slider(Revmat{all}) 40.3 % ( 25 %) Dirichlet(Pi{all}) 38.7 % ( 31 %) Slider(Pi{all}) 73.1 % ( 56 %) Multiplier(Alpha{1,2}) 71.1 % ( 48 %) Multiplier(Alpha{3}) 88.7 % ( 78 %) Slider(Pinvar{all}) 43.5 % ( 39 %) ExtSPR(Tau{all},V{all}) 31.4 % ( 37 %) ExtTBR(Tau{all},V{all}) 46.9 % ( 43 %) NNI(Tau{all},V{all}) 38.0 % ( 34 %) ParsSPR(Tau{all},V{all}) 27.4 % ( 29 %) Multiplier(V{all}) 67.6 % ( 64 %) Nodeslider(V{all}) 26.2 % ( 27 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.62 0.34 0.17 2 | 166858 0.65 0.37 3 | 166533 166669 0.66 4 | 166314 167112 166514 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.63 0.35 0.17 2 | 166858 0.65 0.37 3 | 166430 167039 0.66 4 | 167086 166179 166408 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -621.31 | 1 1 2 222 1 | | 1 | | 2 1 22 21 12 1 2 1 2 | | 2 221 2 2 2 * 2 1 2 2 1 2| |1 2 2 1 2 1 2 2 2 1 1 1 | | 1 1 2 *1 12 1 21 122 1 | | 1 11 2 112 12 1 2 2*2 22 | | 2 1 2 1 1 1 1 1 2 | | 2 1 2 1 2 2 1 * 1 1| | 1 2 1 2 1 2 12 2 2 | |2 1 1 2 1 1 | | 1 1 1 | | 2 | | | | 1 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -625.86 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -617.23 -634.03 2 -617.10 -634.94 -------------------------------------- TOTAL -617.16 -634.58 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.178525 0.001083 0.118449 0.245673 0.175180 1069.38 1285.19 1.000 r(A<->C){all} 0.079549 0.002244 0.005966 0.172616 0.071294 445.81 460.78 1.000 r(A<->G){all} 0.227788 0.004510 0.100509 0.357308 0.222740 523.58 560.73 1.000 r(A<->T){all} 0.063576 0.000874 0.017655 0.122787 0.058889 779.14 808.69 1.000 r(C<->G){all} 0.062269 0.001921 0.000218 0.151444 0.052603 627.76 634.85 1.003 r(C<->T){all} 0.441494 0.006141 0.296266 0.598963 0.442575 433.96 625.34 1.001 r(G<->T){all} 0.125323 0.001945 0.044063 0.214347 0.121603 783.05 819.62 1.001 pi(A){all} 0.261108 0.000659 0.211589 0.310465 0.260066 1085.09 1125.04 1.000 pi(C){all} 0.155686 0.000421 0.118434 0.198902 0.155454 1025.41 1141.73 1.000 pi(G){all} 0.209133 0.000525 0.164448 0.253978 0.208606 1070.75 1168.28 1.000 pi(T){all} 0.374072 0.000751 0.320422 0.427242 0.373577 1176.78 1209.52 1.000 alpha{1,2} 1.183508 0.925405 0.001747 3.072595 0.912898 1243.53 1254.03 1.000 alpha{3} 1.353413 1.133575 0.011997 3.437711 1.069774 906.21 1203.60 1.000 pinvar{all} 0.244839 0.025675 0.000026 0.531946 0.227621 1123.78 1127.93 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C10 3 -- C11 4 -- C12 5 -- C13 6 -- C14 7 -- C15 8 -- C16 9 -- C17 10 -- C2 11 -- C3 12 -- C4 13 -- C5 14 -- C6 15 -- C7 16 -- C8 17 -- C9 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition ----------------------- 1 -- .**************** 2 -- .*............... 3 -- ..*.............. 4 -- ...*............. 5 -- ....*............ 6 -- .....*........... 7 -- ......*.......... 8 -- .......*......... 9 -- ........*........ 10 -- .........*....... 11 -- ..........*...... 12 -- ...........*..... 13 -- ............*.... 14 -- .............*... 15 -- ..............*.. 16 -- ...............*. 17 -- ................* 18 -- ....*.***.*.*..*. 19 -- .*.*.........*... 20 -- .*.************** 21 -- ....*.***.*.*.... 22 -- .....*...*....... 23 -- ....*.***.***..*. 24 -- ....*********..*. 25 -- ....*********.*** 26 -- ...*.........*... 27 -- .*.*............. 28 -- .*...........*... 29 -- ....*********.**. 30 -- ..............*.* 31 -- ....*********..** 32 -- ....*.....*...... 33 -- ......**......... 34 -- ......*.....*.... 35 -- .......*..*...... 36 -- ....*.***.*...... 37 -- ....*.**..*.*.... 38 -- ....*..**.*.*.... 39 -- ......***.*.*.... 40 -- ......*.*........ 41 -- .......**........ 42 -- .......*....*.... 43 -- ....*..*......... 44 -- ........*.*...... 45 -- ..........*.*.... 46 -- ....*.*.*.*.*.... 47 -- ....*.......*.... 48 -- ......*...*...... 49 -- ....*.*.......... 50 -- ....*...*........ 51 -- ....*.***...*.... 52 -- ........*...*.... ----------------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 18 3002 1.000000 0.000000 1.000000 1.000000 2 19 3002 1.000000 0.000000 1.000000 1.000000 2 20 2997 0.998334 0.001413 0.997335 0.999334 2 21 2997 0.998334 0.001413 0.997335 0.999334 2 22 2995 0.997668 0.000471 0.997335 0.998001 2 23 2838 0.945370 0.016959 0.933378 0.957362 2 24 2633 0.877082 0.001413 0.876083 0.878081 2 25 1985 0.661226 0.008009 0.655563 0.666889 2 26 1033 0.344104 0.018373 0.331113 0.357095 2 27 987 0.328781 0.004240 0.325783 0.331779 2 28 982 0.327115 0.014133 0.317122 0.337109 2 29 823 0.274151 0.001413 0.273151 0.275150 2 30 794 0.264490 0.018844 0.251166 0.277815 2 31 782 0.260493 0.003769 0.257828 0.263158 2 32 358 0.119254 0.006595 0.114590 0.123917 2 33 355 0.118254 0.004240 0.115256 0.121252 2 34 353 0.117588 0.000471 0.117255 0.117921 2 35 350 0.116589 0.009422 0.109927 0.123251 2 36 350 0.116589 0.015075 0.105929 0.127249 2 37 347 0.115590 0.001413 0.114590 0.116589 2 38 343 0.114257 0.005182 0.110593 0.117921 2 39 341 0.113591 0.007066 0.108594 0.118588 2 40 336 0.111925 0.004711 0.108594 0.115256 2 41 335 0.111592 0.012719 0.102598 0.120586 2 42 334 0.111259 0.010364 0.103931 0.118588 2 43 333 0.110926 0.006124 0.106596 0.115256 2 44 333 0.110926 0.002355 0.109260 0.112592 2 45 327 0.108927 0.002355 0.107262 0.110593 2 46 326 0.108594 0.003769 0.105929 0.111259 2 47 324 0.107928 0.001884 0.106596 0.109260 2 48 323 0.107595 0.004240 0.104597 0.110593 2 49 321 0.106929 0.005182 0.103264 0.110593 2 50 319 0.106262 0.014604 0.095936 0.116589 2 51 317 0.105596 0.006124 0.101266 0.109927 2 52 315 0.104930 0.000471 0.104597 0.105263 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.002270 0.000005 0.000003 0.006859 0.001547 1.000 2 length{all}[2] 0.002302 0.000006 0.000002 0.007394 0.001522 1.001 2 length{all}[3] 0.002324 0.000006 0.000000 0.006979 0.001592 1.001 2 length{all}[4] 0.002326 0.000006 0.000002 0.007321 0.001524 1.000 2 length{all}[5] 0.002299 0.000006 0.000000 0.006926 0.001600 1.000 2 length{all}[6] 0.011518 0.000033 0.002099 0.023027 0.010666 1.000 2 length{all}[7] 0.002343 0.000006 0.000000 0.007054 0.001629 1.000 2 length{all}[8] 0.002324 0.000006 0.000000 0.007166 0.001561 1.000 2 length{all}[9] 0.002270 0.000005 0.000000 0.006999 0.001566 1.000 2 length{all}[10] 0.023514 0.000077 0.008539 0.040602 0.022462 1.000 2 length{all}[11] 0.002217 0.000005 0.000001 0.006685 0.001531 1.000 2 length{all}[12] 0.004888 0.000013 0.000076 0.011814 0.004065 1.000 2 length{all}[13] 0.002282 0.000005 0.000000 0.006920 0.001567 1.000 2 length{all}[14] 0.002289 0.000006 0.000001 0.006806 0.001500 1.000 2 length{all}[15] 0.007064 0.000018 0.000551 0.015183 0.006172 1.000 2 length{all}[16] 0.002344 0.000006 0.000001 0.007292 0.001552 1.000 2 length{all}[17] 0.026729 0.000082 0.009795 0.043864 0.025616 1.000 2 length{all}[18] 0.011752 0.000032 0.002776 0.022797 0.010844 1.000 2 length{all}[19] 0.012840 0.000037 0.002969 0.025000 0.011839 1.002 2 length{all}[20] 0.007752 0.000022 0.000533 0.016836 0.006815 1.000 2 length{all}[21] 0.007113 0.000019 0.000575 0.015323 0.006357 1.000 2 length{all}[22] 0.010030 0.000029 0.001413 0.020847 0.009084 1.000 2 length{all}[23] 0.005131 0.000015 0.000198 0.012363 0.004198 1.000 2 length{all}[24] 0.004787 0.000012 0.000013 0.011395 0.003979 1.001 2 length{all}[25] 0.004601 0.000013 0.000025 0.011399 0.003676 1.000 2 length{all}[26] 0.002422 0.000007 0.000002 0.007590 0.001578 1.002 2 length{all}[27] 0.002356 0.000006 0.000002 0.006995 0.001592 0.999 2 length{all}[28] 0.002365 0.000006 0.000000 0.007248 0.001574 0.999 2 length{all}[29] 0.002659 0.000007 0.000004 0.007814 0.001812 1.000 2 length{all}[30] 0.002541 0.000007 0.000001 0.007350 0.001772 1.000 2 length{all}[31] 0.002449 0.000007 0.000001 0.007725 0.001603 1.000 2 length{all}[32] 0.002456 0.000006 0.000003 0.006882 0.001706 1.006 2 length{all}[33] 0.002413 0.000006 0.000011 0.007277 0.001718 0.998 2 length{all}[34] 0.002203 0.000005 0.000008 0.006276 0.001530 0.997 2 length{all}[35] 0.002294 0.000005 0.000003 0.006795 0.001666 0.997 2 length{all}[36] 0.002426 0.000006 0.000037 0.007355 0.001760 0.998 2 length{all}[37] 0.002477 0.000006 0.000006 0.007483 0.001710 0.997 2 length{all}[38] 0.002335 0.000004 0.000007 0.006948 0.001716 1.000 2 length{all}[39] 0.002289 0.000006 0.000008 0.006442 0.001490 0.998 2 length{all}[40] 0.002288 0.000005 0.000000 0.006568 0.001600 1.003 2 length{all}[41] 0.002470 0.000006 0.000025 0.007979 0.001693 1.004 2 length{all}[42] 0.002470 0.000006 0.000001 0.007366 0.001826 1.002 2 length{all}[43] 0.002528 0.000006 0.000012 0.007152 0.001933 0.997 2 length{all}[44] 0.002401 0.000006 0.000004 0.007057 0.001748 0.997 2 length{all}[45] 0.002480 0.000005 0.000008 0.006783 0.001808 0.999 2 length{all}[46] 0.002478 0.000006 0.000001 0.006440 0.001799 1.001 2 length{all}[47] 0.002083 0.000005 0.000001 0.007215 0.001445 1.007 2 length{all}[48] 0.002651 0.000009 0.000009 0.008022 0.001765 1.005 2 length{all}[49] 0.002327 0.000006 0.000005 0.007132 0.001570 1.014 2 length{all}[50] 0.002335 0.000006 0.000000 0.007069 0.001583 0.998 2 length{all}[51] 0.002211 0.000005 0.000003 0.006760 0.001573 1.007 2 length{all}[52] 0.002254 0.000006 0.000005 0.006155 0.001513 0.998 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.006138 Maximum standard deviation of split frequencies = 0.018844 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.014 Clade credibility values: /----------------------------------------------------------------------- C1 (1) | |----------------------------------------------------------------------- C11 (3) | | /---------- C10 (2) | | | /------------------------100-----------------------+---------- C12 (4) | | | | | \---------- C6 (14) + | | | /---------- C13 (5) | | | | | |---------- C15 (7) | | | | | |---------- C16 (8) | | /---100---+ | | | |---------- C17 (9) | | | | \---100---+ | |---------- C3 (11) | /---100---+ | | | | \---------- C5 (13) | | | | /----95----+ \-------------------- C8 (16) | | | | | \------------------------------ C4 (12) | /----88---+ | | | /---------- C14 (6) | | \--------------100-------------+ | | \---------- C2 (10) \----66---+ |--------------------------------------------------- C7 (15) | \--------------------------------------------------- C9 (17) Phylogram (based on average branch lengths): /-- C1 (1) | |-- C11 (3) | | /-- C10 (2) | | | /-----------------+-- C12 (4) | | | | | \-- C6 (14) + | | | /--- C13 (5) | | | | | |--- C15 (7) | | | | | |--- C16 (8) | | /--------+ | | | |--- C17 (9) | | | | \----------+ | |--- C3 (11) | /----------------+ | | | | \--- C5 (13) | | | | /------+ \-- C8 (16) | | | | | \------ C4 (12) | /-----+ | | | /----------------- C14 (6) | | \-------------+ | | \----------------------------------- C2 (10) \----+ |---------- C7 (15) | \---------------------------------------- C9 (17) |--------------| 0.010 expected changes per site Calculating tree probabilities... Credible sets of trees (2816 trees sampled): 50 % credible set contains 1315 trees 90 % credible set contains 2516 trees 95 % credible set contains 2666 trees 99 % credible set contains 2786 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C11,((C10,C12,C6),(((((C13,C15,C16,C17,C3,C5),C8),C4),(C14,C2)),C7,C9)))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **17** sequences, **88** codons, and **1** partitions from `/data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/results/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/results/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/results/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -601.28, AIC-c = 1269.07 (33 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.169 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 0.714 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 58 | 1 | 1.049 | 9.055 | Pos. posterior = 0.9055 | ---- ## FUBAR inferred 1 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.09 are expected to be false positives (95% confidence interval of 0-1 )
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.07 sec, SCORE=1000, Nseq=17, Len=88 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIFAVCLMVTIIVVAFLASIKLCIQLCGLCNTL JHM_WU_E_AFO11520_1_1947_08_14_USA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL MHV_1_NA_ACN89738_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL JHM_WU_Dns2_E_AFO11510_1_1947_08_14_USA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL MHV_2_NA_AAF19391_1_NA_NA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL MHV_3_NA_ACN89744_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL MHV_JHM_IA_NA_ACN89766_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL MHV_MI_E_BAJ04699_1_1994_Australia_Mouse_Murine_coronavirus MFNVLSTDTVWYVGQIIFIVAVCLMVTIVVVAFLASIKLCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus0 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus1 MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus2 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus3 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus4 MFNLFLTDTVWYVGQIIFIVAVCLMVTITVVAFLASIKLCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus5 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus6 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus7 MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL ***:: ************.******** ********* *********** 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus VLSPSIYLYDRSKQLYKYYNEEMRMPLLEVDDI----- JHM_WU_E_AFO11520_1_1947_08_14_USA_Unknown_Murine_coronavirus LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL MHV_1_NA_ACN89738_1_NA_USA_Mouse_Murine_coronavirus LLSPSIYVYNGSKQLYKYYNEEVRPPPLEVDDKIIQTL JHM_WU_Dns2_E_AFO11510_1_1947_08_14_USA_Unknown_Murine_coronavirus LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL MHV_2_NA_AAF19391_1_NA_NA_Unknown_Murine_coronavirus LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL MHV_3_NA_ACN89744_1_NA_USA_Mouse_Murine_coronavirus LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL MHV_JHM_IA_NA_ACN89766_1_NA_USA_Mouse_Murine_coronavirus LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL MHV_MI_E_BAJ04699_1_1994_Australia_Mouse_Murine_coronavirus LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus0 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus1 LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus2 LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus3 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus4 VLSPSIYLYDRSKQLYKYYNEEVRPPPLEVDDIIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus5 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus6 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus7 LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL :***** :*: ***********:* * *****
>8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus ATGTTTAATTTATTCCTTATAGACACAGTATGGTACGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACTTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCTCCTTCTATTTATGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATTTTTATATTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATCAAACTTTGTATTCAACTTTGCGGTTTATGTAATACTTTGGTGCTGTCCCCTTCTATTTATTTGTATGATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAATGAGAATGCCCCTATTAGAGGTGGATGATATC--------------- >JHM_WU_E_AFO11520_1_1947_08_14_USA_Unknown_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATCTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA >MHV_1_NA_ACN89738_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACTTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCCCCTTCTATTTATGTGTATAATGGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAAAATAATCCAAACATTA >JHM_WU_Dns2_E_AFO11510_1_1947_08_14_USA_Unknown_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATCTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA >MHV_2_NA_AAF19391_1_NA_NA_Unknown_Murine_coronavirus ATGTTTAATTTATTCCTTACAGATACAGTATGGTATGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATAGTGGTTGCCTTCCTTGCGTCTATCAAACTTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCTCCTTCTATTTGTGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >MHV_3_NA_ACN89744_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCCCCTTCTATTTATGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGGCCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >MHV_JHM_IA_NA_ACN89766_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACTTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA >MHV_MI_E_BAJ04699_1_1994_Australia_Mouse_Murine_coronavirus ATGTTTAATGTACTCTCTACAGACACAGTATGGTATGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTAATGGTCACTATAGTTGTGGTCGCTTTCCTTGCGTCTATTAAACTTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCCCCTTCTATTTATGTGTATAATAGGAGTAAGCAGCTATATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >ML_11_orf5B_AAF68928_1_NA_NA_Unknown_Murine_coronavirus ATGTTTAATTTATTCCTTACAGATACAGTATGGTATGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATAGTGGTTGCCTTCCTTGCGTCTATCAAACTTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCTCCTTCTATTTGTGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >Parker_E_YP_003029850_1_NA_NA_Rat_Murine_coronavirus ATGTTTAATTTATTCCTTATAGACACAGTATGGTACGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACTTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCTCCTTCTATTTATGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >Penn_97_1_ORF5B_AAF69336_1_NA_NA_Unknown_Murine_coronavirus ATGTTTAATTTATTCCTTACAGATACAGTATGGTATGTGGGGCAGATTATTTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATAGTGGTTGCCTTCCTTGCGTCTATCAAACTTTGTATTCAACTTTGCGGTTTGTGTAATACTTTGTTGCTGTCTCCTTCTATTTGTGTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >RJHM_A_NA_ACN89698_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATCTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA >S_E_ADI59791_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTTACCATAACTGTGGTTGCCTTCCTTGCGTCTATTAAACTTTGTATTCAACTTTGCGGTTTATGTAATACTTTGGTGCTGTCCCCTTCTATTTATTTGTATGATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTTAGACCGCCCCCGTTAGAGGTGGATGATATAATAATCCAAACATTA >SA59_RJHM_NA_ACN89726_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATCTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA >UNKNOWN_AC_000192_E_YP_209236_1_NA_NA_Unknown_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATCTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA >repA59_RJHM_NA_ACN89719_1_NA_USA_Mouse_Murine_coronavirus ATGTTTAATTTATTCCTTACAGACACAGTATGGTATGTGGGGCAGATTATCTTTATAGTCGCAGTGTGTTTGATGGTCACCATAATTGTGGTTGCCTTCCTTGCGTCTATTAAACGTTGTATTCAACTTTGCGGTTTATGTAATACTTTGTTGCTGTCTCCCTCTATTTATCTGTATAATAGGAGTAAGCAGCTTTATAAGTATTATAATGAAGAAGTGAGACCGCCCCCGTTAGAGGTGGATGATAATATAATCCAAACATTA
>8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIFAVCLMVTIIVVAFLASIKLCIQLCGLCNTL VLSPSIYLYDRSKQLYKYYNEEMRMPLLEVDDI----- >JHM_WU_E_AFO11520_1_1947_08_14_USA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL >MHV_1_NA_ACN89738_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSIYVYNGSKQLYKYYNEEVRPPPLEVDDKIIQTL >JHM_WU_Dns2_E_AFO11510_1_1947_08_14_USA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL >MHV_2_NA_AAF19391_1_NA_NA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >MHV_3_NA_ACN89744_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >MHV_JHM_IA_NA_ACN89766_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL >MHV_MI_E_BAJ04699_1_1994_Australia_Mouse_Murine_coronavirus MFNVLSTDTVWYVGQIIFIVAVCLMVTIVVVAFLASIKLCIQLCGLCNTL LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >ML_11_orf5B_AAF68928_1_NA_NA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >Parker_E_YP_003029850_1_NA_NA_Rat_Murine_coronavirus MFNLFLIDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSIYVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >Penn_97_1_ORF5B_AAF69336_1_NA_NA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKLCIQLCGLCNTL LLSPSICVYNRSKQLYKYYNEEVRPPPLEVDDIIIQTL >RJHM_A_NA_ACN89698_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL >S_E_ADI59791_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTITVVAFLASIKLCIQLCGLCNTL VLSPSIYLYDRSKQLYKYYNEEVRPPPLEVDDIIIQTL >SA59_RJHM_NA_ACN89726_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL >UNKNOWN_AC_000192_E_YP_209236_1_NA_NA_Unknown_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL >repA59_RJHM_NA_ACN89719_1_NA_USA_Mouse_Murine_coronavirus MFNLFLTDTVWYVGQIIFIVAVCLMVTIIVVAFLASIKRCIQLCGLCNTL LLSPSIYLYNRSKQLYKYYNEEVRPPPLEVDDNIIQTL
Reading sequence file /data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/fasta/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1 Found 17 sequences of length 264 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 3.4% Found 16 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 5 Calculating analytical mean and variance Doing permutation test for PHI Doing permutation test for NSS Doing Permutation test for MAXCHI Writing alignment of polymorphic unambig sites to: Phi.poly.sites Window size is 24 polymorphic sites **p-Value(s)** ---------- NSS: 2.28e-01 (1000 permutations) Max Chi^2: 4.20e-01 (1000 permutations) PHI (Permutation): 6.58e-01 (1000 permutations) PHI (Normal): 5.51e-01
#NEXUS [ID: 8018085733] begin taxa; dimensions ntax=17; taxlabels 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus ML_11_orf5B_AAF68928_1_NA_NA_Unknown_Murine_coronavirus Parker_E_YP_003029850_1_NA_NA_Rat_Murine_coronavirus Penn_97_1_ORF5B_AAF69336_1_NA_NA_Unknown_Murine_coronavirus RJHM_A_NA_ACN89698_1_NA_USA_Mouse_Murine_coronavirus S_E_ADI59791_1_NA_USA_Mouse_Murine_coronavirus SA59_RJHM_NA_ACN89726_1_NA_USA_Mouse_Murine_coronavirus UNKNOWN_AC_000192_E_YP_209236_1_NA_NA_Unknown_Murine_coronavirus repA59_RJHM_NA_ACN89719_1_NA_USA_Mouse_Murine_coronavirus A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus JHM_WU_E_AFO11520_1_1947_08_14_USA_Unknown_Murine_coronavirus MHV_1_NA_ACN89738_1_NA_USA_Mouse_Murine_coronavirus JHM_WU_Dns2_E_AFO11510_1_1947_08_14_USA_Unknown_Murine_coronavirus MHV_2_NA_AAF19391_1_NA_NA_Unknown_Murine_coronavirus MHV_3_NA_ACN89744_1_NA_USA_Mouse_Murine_coronavirus MHV_JHM_IA_NA_ACN89766_1_NA_USA_Mouse_Murine_coronavirus MHV_MI_E_BAJ04699_1_1994_Australia_Mouse_Murine_coronavirus ; end; begin trees; translate 1 8190_NA_AFG25767_1_NA_NA_Rat_Murine_coronavirus, 2 ML_11_orf5B_AAF68928_1_NA_NA_Unknown_Murine_coronavirus, 3 Parker_E_YP_003029850_1_NA_NA_Rat_Murine_coronavirus, 4 Penn_97_1_ORF5B_AAF69336_1_NA_NA_Unknown_Murine_coronavirus, 5 RJHM_A_NA_ACN89698_1_NA_USA_Mouse_Murine_coronavirus, 6 S_E_ADI59791_1_NA_USA_Mouse_Murine_coronavirus, 7 SA59_RJHM_NA_ACN89726_1_NA_USA_Mouse_Murine_coronavirus, 8 UNKNOWN_AC_000192_E_YP_209236_1_NA_NA_Unknown_Murine_coronavirus, 9 repA59_RJHM_NA_ACN89719_1_NA_USA_Mouse_Murine_coronavirus, 10 A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus, 11 JHM_WU_E_AFO11520_1_1947_08_14_USA_Unknown_Murine_coronavirus, 12 MHV_1_NA_ACN89738_1_NA_USA_Mouse_Murine_coronavirus, 13 JHM_WU_Dns2_E_AFO11510_1_1947_08_14_USA_Unknown_Murine_coronavirus, 14 MHV_2_NA_AAF19391_1_NA_NA_Unknown_Murine_coronavirus, 15 MHV_3_NA_ACN89744_1_NA_USA_Mouse_Murine_coronavirus, 16 MHV_JHM_IA_NA_ACN89766_1_NA_USA_Mouse_Murine_coronavirus, 17 MHV_MI_E_BAJ04699_1_1994_Australia_Mouse_Murine_coronavirus ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:1.546888e-03,3:1.591628e-03,((2:1.521750e-03,4:1.524188e-03,14:1.500320e-03)1.000:1.183904e-02,(((((5:1.599755e-03,7:1.629410e-03,8:1.561086e-03,9:1.565986e-03,11:1.531027e-03,13:1.566756e-03)0.998:6.357044e-03,16:1.552424e-03)1.000:1.084438e-02,12:4.065261e-03)0.945:4.197988e-03,(6:1.066602e-02,10:2.246192e-02)0.998:9.083714e-03)0.877:3.979489e-03,15:6.171795e-03,17:2.561557e-02)0.661:3.676336e-03)0.998:6.814817e-03); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:1.546888e-03,3:1.591628e-03,((2:1.521750e-03,4:1.524188e-03,14:1.500320e-03):1.183904e-02,(((((5:1.599755e-03,7:1.629410e-03,8:1.561086e-03,9:1.565986e-03,11:1.531027e-03,13:1.566756e-03):6.357044e-03,16:1.552424e-03):1.084438e-02,12:4.065261e-03):4.197988e-03,(6:1.066602e-02,10:2.246192e-02):9.083714e-03):3.979489e-03,15:6.171795e-03,17:2.561557e-02):3.676336e-03):6.814817e-03); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -617.48 -634.77 2 -617.23 -636.92 -------------------------------------- TOTAL -617.34 -636.34 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.178313 0.001050 0.116801 0.239773 0.175271 1303.08 1402.04 1.000 r(A<->C){all} 0.079048 0.002041 0.009457 0.170477 0.071840 467.36 537.39 1.000 r(A<->G){all} 0.225054 0.004421 0.103278 0.355008 0.219789 438.36 505.03 1.001 r(A<->T){all} 0.064725 0.000963 0.013930 0.127309 0.059625 648.09 709.40 1.000 r(C<->G){all} 0.065099 0.002079 0.000015 0.154581 0.055015 502.87 512.51 1.000 r(C<->T){all} 0.439084 0.006526 0.281725 0.595534 0.437984 455.10 550.56 1.001 r(G<->T){all} 0.126991 0.002040 0.042310 0.209926 0.121780 811.66 890.80 1.000 pi(A){all} 0.261889 0.000657 0.210931 0.310406 0.261649 1003.58 1032.23 1.002 pi(C){all} 0.155242 0.000433 0.116419 0.196806 0.154836 1096.47 1124.74 1.000 pi(G){all} 0.209123 0.000563 0.163449 0.255496 0.209300 1063.42 1092.50 1.000 pi(T){all} 0.373746 0.000812 0.319034 0.431979 0.373304 1090.53 1183.59 1.001 alpha{1,2} 1.185373 0.943523 0.003009 3.161392 0.895914 1261.56 1381.28 1.001 alpha{3} 1.318890 0.943571 0.001626 3.242017 1.073839 1117.90 1195.80 1.000 pinvar{all} 0.236886 0.024629 0.000053 0.515873 0.222638 724.47 828.14 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C11,((C10,C12,C6),(((((C13,C15,C16,C17,C3,C5),C8),C4),(C14,C2)),C7,C9)))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **17** sequences, **88** codons, and **1** partitions from `/data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/results/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/results/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result/original_alignment/fubar/results/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1/A59_NA_ATN37890_1_2014_01_16_USA_Mouse_Murine_coronavirus.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -601.28, AIC-c = 1269.07 (33 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.169 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 0.714 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 58 | 1 | 1.049 | 9.055 | Pos. posterior = 0.9055 | ---- ## FUBAR inferred 1 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.09 are expected to be false positives (95% confidence interval of 0-1 )
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500