--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -854.97 -866.31 2 -854.96 -866.16 -------------------------------------- TOTAL -854.97 -866.24 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.093203 0.000358 0.058858 0.131155 0.090837 1347.16 1424.08 1.000 r(A<->C){all} 0.082698 0.001753 0.015341 0.167786 0.076230 677.63 754.93 1.002 r(A<->G){all} 0.253154 0.005514 0.122308 0.407673 0.247810 340.57 371.16 1.000 r(A<->T){all} 0.082169 0.001618 0.014997 0.161858 0.075938 595.54 642.39 1.001 r(C<->G){all} 0.043326 0.001472 0.000018 0.118620 0.032519 467.44 515.63 1.003 r(C<->T){all} 0.390746 0.006680 0.231735 0.543070 0.388783 454.16 539.93 1.000 r(G<->T){all} 0.147908 0.003503 0.037087 0.260888 0.141226 590.27 620.87 1.007 pi(A){all} 0.295606 0.000421 0.256404 0.335086 0.294951 1199.82 1325.62 1.000 pi(C){all} 0.225250 0.000345 0.189409 0.262090 0.224890 1248.34 1254.17 1.000 pi(G){all} 0.193895 0.000330 0.157420 0.228435 0.193361 980.79 1184.40 1.000 pi(T){all} 0.285249 0.000404 0.248145 0.325299 0.284923 1177.44 1200.22 1.000 alpha{1,2} 0.703206 0.615702 0.000198 2.241400 0.441514 1152.55 1212.30 1.000 alpha{3} 1.324228 1.223212 0.000646 3.485764 1.019498 1202.04 1314.42 1.000 pinvar{all} 0.371197 0.041543 0.004650 0.708667 0.368601 727.96 808.22 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Thu Oct 20 00:46:21 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.05 sec, SCORE=1000, Nseq=8, Len=153 C1 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C2 MKLLLLLSILSFSSAAPTTYRASQAAKVLIHTTEKVTLNNQRTHSYTKWS C3 MKLLLLLSILSFSSAAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C4 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C5 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C6 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C7 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C8 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG *********:*** :***************:************:*****. C1 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C2 VCSTGWNTYTNTMVVVNGRWVETAKPPKPTAVAIPTFPYEPRSEKPGFGS C3 VCSTGWNTYTNTMVVVNGRWVETTKPPRPTAIAIPTFPYEPRSEKPGFGS C4 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C5 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C6 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS C7 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS C8 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS ***********************:***.***.****.*** ********* C1 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C2 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWST C3 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHQTFAVTTFRWST C4 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C5 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C6 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS C7 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS C8 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS ***********:***.**** *****************:*******:**: C1 PLN C2 PSN C3 PSN C4 PSN C5 PSN C6 PSN C7 PSN C8 PSN * * -- Starting log on Thu Oct 20 00:46:44 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=8, Len=153 C1 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C2 MKLLLLLSILSFSSAAPTTYRASQAAKVLIHTTEKVTLNNQRTHSYTKWS C3 MKLLLLLSILSFSSAAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C4 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C5 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C6 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C7 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C8 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG *********:*** :***************:************:*****. C1 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C2 VCSTGWNTYTNTMVVVNGRWVETAKPPKPTAVAIPTFPYEPRSEKPGFGS C3 VCSTGWNTYTNTMVVVNGRWVETTKPPRPTAIAIPTFPYEPRSEKPGFGS C4 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C5 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C6 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS C7 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS C8 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS ***********************:***.***.****.*** ********* C1 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C2 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWST C3 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHQTFAVTTFRWST C4 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C5 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C6 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS C7 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS C8 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS ***********:***.**** *****************:*******:**: C1 PLN C2 PSN C3 PSN C4 PSN C5 PSN C6 PSN C7 PSN C8 PSN * * -- Starting log on Thu Oct 20 00:46:21 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.05 sec, SCORE=1000, Nseq=8, Len=153 C1 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C2 MKLLLLLSILSFSSAAPTTYRASQAAKVLIHTTEKVTLNNQRTHSYTKWS C3 MKLLLLLSILSFSSAAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C4 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C5 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG C6 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C7 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG C8 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG *********:*** :***************:************:*****. C1 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C2 VCSTGWNTYTNTMVVVNGRWVETAKPPKPTAVAIPTFPYEPRSEKPGFGS C3 VCSTGWNTYTNTMVVVNGRWVETTKPPRPTAIAIPTFPYEPRSEKPGFGS C4 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C5 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS C6 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS C7 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS C8 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS ***********************:***.***.****.*** ********* C1 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C2 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWST C3 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHQTFAVTTFRWST C4 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C5 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS C6 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS C7 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS C8 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS ***********:***.**** *****************:*******:**: C1 PLN C2 PSN C3 PSN C4 PSN C5 PSN C6 PSN C7 PSN C8 PSN * * -- Starting log on Thu Oct 20 00:57:32 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/gapped_alignment/fubar,175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 8 taxa and 459 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C2 Taxon 3 -> C3 Taxon 4 -> C4 Taxon 5 -> C5 Taxon 6 -> C6 Taxon 7 -> C7 Taxon 8 -> C8 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1666227456 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 1936132395 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 5175601111 Seed = 1949362178 Swapseed = 1666227456 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 15 unique site patterns Division 2 has 10 unique site patterns Division 3 has 15 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -1055.039611 -- 29.153684 Chain 2 -- -1047.307883 -- 29.153684 Chain 3 -- -1054.507992 -- 29.153684 Chain 4 -- -1065.898740 -- 29.153684 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -1060.882604 -- 29.153684 Chain 2 -- -1048.174008 -- 29.153684 Chain 3 -- -1053.851002 -- 29.153684 Chain 4 -- -1007.168667 -- 29.153684 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-1055.040] (-1047.308) (-1054.508) (-1065.899) * [-1060.883] (-1048.174) (-1053.851) (-1007.169) 1000 -- (-867.629) (-870.951) (-867.480) [-869.131] * (-867.916) (-869.300) (-862.673) [-862.092] -- 0:00:00 2000 -- (-865.877) [-861.825] (-868.362) (-867.726) * (-861.554) (-869.962) [-856.094] (-860.227) -- 0:00:00 3000 -- (-863.668) [-857.907] (-864.108) (-863.422) * (-863.541) [-869.057] (-865.440) (-862.991) -- 0:05:32 4000 -- (-863.641) (-867.869) (-867.970) [-860.875] * (-866.085) (-877.182) [-855.558] (-857.483) -- 0:04:09 5000 -- (-862.163) [-860.720] (-866.715) (-859.426) * (-857.654) (-867.263) [-856.388] (-866.875) -- 0:03:19 Average standard deviation of split frequencies: 0.132960 6000 -- [-859.314] (-863.184) (-872.656) (-862.158) * [-861.449] (-869.637) (-856.891) (-866.396) -- 0:02:45 7000 -- [-856.712] (-860.742) (-862.205) (-864.715) * (-863.488) [-866.736] (-859.747) (-862.118) -- 0:02:21 8000 -- (-856.480) (-855.865) (-866.509) [-861.190] * (-859.462) (-861.283) [-858.369] (-875.916) -- 0:04:08 9000 -- (-861.116) (-855.565) [-864.238] (-860.761) * (-862.701) (-864.115) [-857.867] (-866.098) -- 0:03:40 10000 -- (-857.604) (-858.346) (-858.810) [-862.818] * (-864.111) (-860.958) [-858.120] (-867.447) -- 0:03:18 Average standard deviation of split frequencies: 0.081589 11000 -- (-859.583) (-858.622) [-857.306] (-859.648) * (-863.976) (-862.218) (-864.730) [-860.009] -- 0:02:59 12000 -- [-856.238] (-861.118) (-855.584) (-860.167) * [-862.907] (-858.340) (-859.938) (-864.336) -- 0:02:44 13000 -- [-859.262] (-854.607) (-865.766) (-864.506) * (-859.963) [-857.017] (-859.524) (-864.386) -- 0:03:47 14000 -- (-858.114) [-856.837] (-864.324) (-868.884) * (-858.899) (-864.654) (-853.267) [-853.718] -- 0:03:31 15000 -- (-864.954) (-856.840) [-861.382] (-858.364) * (-858.885) (-857.903) (-862.350) [-855.514] -- 0:03:17 Average standard deviation of split frequencies: 0.045327 16000 -- [-859.090] (-876.564) (-857.751) (-856.972) * [-854.714] (-857.935) (-853.147) (-861.908) -- 0:03:04 17000 -- (-866.586) (-859.752) (-864.319) [-853.785] * (-859.910) (-860.568) (-854.367) [-856.714] -- 0:02:53 18000 -- (-865.461) (-859.572) (-858.005) [-860.978] * (-856.513) (-859.234) [-859.879] (-858.500) -- 0:03:38 19000 -- (-855.017) (-861.373) [-857.206] (-863.005) * (-861.126) [-856.130] (-862.022) (-858.026) -- 0:03:26 20000 -- (-861.553) (-861.823) (-859.387) [-861.002] * [-858.490] (-856.147) (-870.709) (-857.091) -- 0:03:16 Average standard deviation of split frequencies: 0.038601 21000 -- [-858.980] (-868.482) (-863.921) (-860.877) * (-858.903) (-858.668) (-863.506) [-859.497] -- 0:03:06 22000 -- (-864.080) (-870.862) [-859.569] (-865.773) * (-863.556) [-858.109] (-864.182) (-862.862) -- 0:02:57 23000 -- [-858.408] (-868.607) (-864.755) (-861.405) * [-862.180] (-864.044) (-862.936) (-864.666) -- 0:03:32 24000 -- (-864.231) (-857.434) (-861.006) [-855.719] * (-863.719) (-860.474) (-858.121) [-859.974] -- 0:03:23 25000 -- (-859.405) (-860.531) [-851.822] (-860.410) * [-862.626] (-868.636) (-860.134) (-859.238) -- 0:03:15 Average standard deviation of split frequencies: 0.039051 26000 -- (-858.605) [-857.743] (-862.586) (-856.533) * (-865.933) (-861.286) (-873.290) [-857.554] -- 0:03:07 27000 -- [-865.779] (-862.857) (-866.467) (-858.164) * (-862.348) (-869.523) (-864.110) [-855.179] -- 0:03:00 28000 -- [-853.272] (-859.306) (-866.230) (-860.468) * [-860.310] (-866.233) (-871.860) (-857.581) -- 0:03:28 29000 -- [-854.461] (-864.314) (-854.966) (-861.491) * (-866.331) (-859.199) (-869.485) [-860.129] -- 0:03:20 30000 -- (-856.822) (-856.544) [-857.573] (-864.012) * (-855.088) (-856.188) (-866.864) [-855.699] -- 0:03:14 Average standard deviation of split frequencies: 0.037838 31000 -- (-865.550) (-854.185) [-855.257] (-859.044) * (-859.912) (-859.374) (-863.216) [-855.661] -- 0:03:07 32000 -- (-864.285) [-860.609] (-862.008) (-863.369) * [-857.900] (-862.526) (-859.300) (-856.590) -- 0:03:01 33000 -- (-865.148) (-868.209) [-858.735] (-857.661) * [-858.203] (-860.739) (-863.775) (-859.914) -- 0:03:25 34000 -- (-859.180) (-861.259) (-857.821) [-856.541] * [-860.630] (-863.406) (-863.933) (-862.971) -- 0:03:18 35000 -- [-855.154] (-863.288) (-868.756) (-860.953) * [-854.891] (-861.013) (-866.705) (-866.487) -- 0:03:13 Average standard deviation of split frequencies: 0.040291 36000 -- (-856.773) [-867.743] (-864.990) (-854.959) * [-858.787] (-874.721) (-861.334) (-863.676) -- 0:03:07 37000 -- (-856.025) (-867.583) [-857.036] (-856.428) * [-856.055] (-859.049) (-868.520) (-865.967) -- 0:03:02 38000 -- [-855.282] (-863.854) (-864.410) (-859.277) * [-858.574] (-861.087) (-871.576) (-854.865) -- 0:03:22 39000 -- (-864.475) (-871.415) (-857.888) [-858.452] * [-859.319] (-861.383) (-864.898) (-861.299) -- 0:03:17 40000 -- (-858.238) [-857.716] (-858.540) (-860.205) * (-857.023) [-857.530] (-861.051) (-867.406) -- 0:03:12 Average standard deviation of split frequencies: 0.021400 41000 -- (-857.977) (-862.778) [-862.957] (-862.663) * (-858.701) [-854.198] (-857.141) (-861.781) -- 0:03:07 42000 -- (-862.049) (-863.362) (-862.572) [-859.275] * [-865.294] (-864.657) (-862.134) (-869.955) -- 0:03:02 43000 -- (-860.797) [-857.614] (-860.403) (-865.589) * [-868.240] (-860.728) (-862.066) (-855.140) -- 0:03:20 44000 -- (-862.399) [-858.589] (-863.516) (-860.632) * (-862.245) (-858.248) [-858.336] (-862.290) -- 0:03:15 45000 -- (-857.994) (-863.919) [-860.958] (-862.155) * (-868.442) (-858.913) [-859.435] (-857.714) -- 0:03:11 Average standard deviation of split frequencies: 0.013401 46000 -- [-864.412] (-855.289) (-866.464) (-863.253) * [-854.463] (-864.280) (-860.430) (-858.021) -- 0:03:06 47000 -- (-867.010) (-860.059) [-868.559] (-856.697) * (-856.743) (-865.801) (-861.266) [-860.523] -- 0:03:02 48000 -- (-866.022) [-856.794] (-862.393) (-858.132) * [-856.187] (-874.150) (-858.562) (-861.966) -- 0:03:18 49000 -- (-855.565) (-860.680) (-862.240) [-857.546] * (-860.943) (-866.627) (-860.774) [-858.037] -- 0:03:14 50000 -- (-854.281) (-859.785) (-865.806) [-860.340] * (-854.081) [-866.108] (-861.175) (-868.058) -- 0:03:10 Average standard deviation of split frequencies: 0.014314 51000 -- [-855.177] (-860.026) (-867.349) (-863.512) * [-856.620] (-862.297) (-856.629) (-863.244) -- 0:03:06 52000 -- (-863.441) [-855.724] (-863.139) (-856.608) * (-859.667) (-865.152) [-852.322] (-860.456) -- 0:03:02 53000 -- (-861.626) [-863.780] (-872.181) (-868.214) * [-860.755] (-865.207) (-867.791) (-859.163) -- 0:03:16 54000 -- (-868.656) [-863.871] (-869.119) (-859.846) * (-862.259) (-864.053) (-860.816) [-854.996] -- 0:03:12 55000 -- (-858.775) [-860.966] (-862.895) (-871.226) * (-859.067) (-861.106) (-852.717) [-859.900] -- 0:03:09 Average standard deviation of split frequencies: 0.016836 56000 -- [-861.177] (-863.609) (-869.605) (-866.440) * (-856.883) (-858.461) [-864.316] (-858.612) -- 0:03:05 57000 -- (-866.082) (-856.029) (-862.051) [-864.422] * (-860.477) [-857.883] (-859.072) (-863.837) -- 0:03:01 58000 -- (-851.909) (-854.769) [-857.401] (-866.263) * (-858.962) [-859.283] (-863.325) (-855.194) -- 0:03:14 59000 -- (-858.272) (-850.658) [-861.937] (-854.878) * (-864.037) (-857.649) (-860.118) [-853.852] -- 0:03:11 60000 -- (-856.292) [-854.531] (-858.859) (-858.611) * (-861.468) (-855.418) [-862.192] (-860.156) -- 0:03:08 Average standard deviation of split frequencies: 0.016736 61000 -- (-855.881) (-860.638) (-858.939) [-861.338] * (-862.404) (-860.439) (-855.957) [-865.111] -- 0:03:04 62000 -- [-858.393] (-862.890) (-856.106) (-860.973) * (-857.694) [-863.375] (-868.684) (-856.700) -- 0:03:01 63000 -- [-856.435] (-854.007) (-863.045) (-866.552) * [-857.947] (-860.228) (-863.437) (-863.222) -- 0:03:13 64000 -- (-862.511) (-861.379) [-860.805] (-855.575) * (-858.954) (-860.063) (-853.213) [-857.882] -- 0:03:10 65000 -- (-857.411) [-863.954] (-866.995) (-864.043) * (-866.003) (-864.788) [-857.958] (-862.743) -- 0:03:07 Average standard deviation of split frequencies: 0.016483 66000 -- (-862.437) (-860.341) (-861.592) [-861.312] * [-863.490] (-858.782) (-858.803) (-859.007) -- 0:03:03 67000 -- [-864.226] (-858.545) (-858.051) (-859.303) * (-860.836) (-867.113) (-860.370) [-855.722] -- 0:03:01 68000 -- [-856.657] (-856.503) (-867.360) (-859.873) * [-860.715] (-862.054) (-860.894) (-858.379) -- 0:03:11 69000 -- (-864.477) [-857.137] (-855.360) (-855.807) * (-857.329) (-864.590) [-860.726] (-860.745) -- 0:03:08 70000 -- (-868.835) (-862.769) (-861.495) [-856.407] * [-858.707] (-863.860) (-857.341) (-868.232) -- 0:03:06 Average standard deviation of split frequencies: 0.017447 71000 -- [-862.673] (-860.031) (-863.792) (-858.340) * [-854.058] (-858.844) (-863.346) (-859.998) -- 0:03:03 72000 -- [-866.330] (-853.534) (-856.790) (-859.480) * (-865.892) [-861.649] (-856.448) (-860.194) -- 0:03:00 73000 -- (-862.530) (-858.337) (-858.802) [-857.100] * (-857.942) [-855.188] (-861.098) (-858.915) -- 0:03:10 74000 -- [-856.461] (-866.230) (-858.080) (-856.899) * (-860.996) [-857.632] (-861.373) (-858.248) -- 0:03:07 75000 -- (-863.259) [-858.864] (-861.172) (-866.455) * [-865.804] (-868.842) (-855.864) (-865.836) -- 0:03:05 Average standard deviation of split frequencies: 0.020994 76000 -- (-861.112) (-857.839) [-862.492] (-859.745) * (-870.321) (-866.641) [-854.832] (-859.095) -- 0:03:02 77000 -- (-863.345) (-859.276) [-858.556] (-867.567) * (-864.725) (-870.560) [-857.909] (-864.866) -- 0:02:59 78000 -- [-857.774] (-865.729) (-859.927) (-860.488) * (-878.581) (-866.988) (-862.259) [-858.246] -- 0:03:09 79000 -- (-862.578) (-854.719) [-853.509] (-867.118) * (-862.631) (-860.971) [-855.444] (-857.845) -- 0:03:06 80000 -- [-857.448] (-862.787) (-868.162) (-866.575) * (-861.310) (-870.893) (-865.435) [-864.221] -- 0:03:04 Average standard deviation of split frequencies: 0.023375 81000 -- (-862.491) (-864.343) [-855.062] (-867.494) * [-856.454] (-866.532) (-859.076) (-862.434) -- 0:03:01 82000 -- (-863.257) (-861.077) [-861.828] (-858.782) * (-862.546) (-858.229) (-860.964) [-854.674] -- 0:02:59 83000 -- (-855.438) [-857.082] (-860.745) (-857.536) * [-857.849] (-858.419) (-863.235) (-858.165) -- 0:03:07 84000 -- (-869.006) (-860.563) [-860.038] (-861.239) * (-859.063) (-856.184) (-861.159) [-855.805] -- 0:03:05 85000 -- [-859.101] (-858.312) (-860.017) (-866.139) * (-858.522) [-860.762] (-868.537) (-864.281) -- 0:03:03 Average standard deviation of split frequencies: 0.024456 86000 -- (-864.344) (-857.016) [-862.516] (-864.161) * (-857.953) (-856.092) (-867.765) [-868.843] -- 0:03:00 87000 -- (-858.209) (-859.395) [-858.288] (-858.633) * (-861.868) [-851.427] (-864.821) (-862.517) -- 0:02:58 88000 -- (-857.874) (-864.188) [-857.284] (-857.139) * (-863.645) (-862.789) (-858.959) [-854.699] -- 0:03:06 89000 -- (-856.664) (-860.528) [-856.875] (-857.446) * (-866.101) (-862.638) (-862.345) [-863.379] -- 0:03:04 90000 -- (-863.577) (-856.693) [-859.614] (-861.566) * (-862.458) (-861.832) (-862.224) [-858.376] -- 0:03:02 Average standard deviation of split frequencies: 0.023197 91000 -- (-860.455) [-855.487] (-866.389) (-866.497) * (-861.536) (-858.835) [-867.915] (-859.261) -- 0:02:59 92000 -- (-863.234) (-856.122) [-863.985] (-856.668) * [-855.081] (-852.889) (-857.258) (-861.250) -- 0:02:57 93000 -- (-862.942) (-858.027) [-868.604] (-857.388) * (-866.654) (-853.234) [-856.302] (-857.206) -- 0:03:05 94000 -- (-857.132) [-864.061] (-867.719) (-861.748) * (-859.673) [-856.765] (-859.292) (-865.716) -- 0:03:03 95000 -- (-855.904) [-856.048] (-867.277) (-866.173) * [-863.957] (-862.532) (-865.012) (-856.032) -- 0:03:01 Average standard deviation of split frequencies: 0.019642 96000 -- (-865.589) (-858.386) (-864.641) [-858.192] * [-863.427] (-870.518) (-862.858) (-857.819) -- 0:02:58 97000 -- (-861.486) [-859.971] (-856.677) (-863.212) * (-857.092) [-857.127] (-866.334) (-857.067) -- 0:02:56 98000 -- (-856.744) (-870.152) (-858.190) [-858.184] * [-853.885] (-871.621) (-859.425) (-861.413) -- 0:03:04 99000 -- (-856.897) [-857.559] (-859.352) (-863.125) * (-867.573) (-858.937) (-862.493) [-855.305] -- 0:03:02 100000 -- (-855.404) [-866.141] (-861.222) (-872.553) * (-857.623) (-865.579) (-864.175) [-855.001] -- 0:03:00 Average standard deviation of split frequencies: 0.015850 101000 -- (-857.469) (-861.179) [-855.518] (-866.693) * (-869.655) (-860.588) (-859.364) [-855.673] -- 0:02:58 102000 -- (-866.025) (-862.403) (-860.989) [-861.957] * [-856.876] (-865.666) (-860.490) (-860.226) -- 0:02:56 103000 -- (-864.445) [-855.121] (-855.843) (-861.686) * (-866.113) (-862.793) (-863.773) [-850.471] -- 0:03:02 104000 -- (-860.589) [-866.342] (-859.139) (-860.692) * (-853.730) (-862.275) [-858.790] (-861.168) -- 0:03:00 105000 -- (-862.848) [-857.727] (-860.284) (-857.828) * (-858.110) (-863.528) [-870.122] (-866.468) -- 0:02:59 Average standard deviation of split frequencies: 0.014368 106000 -- (-861.459) (-869.409) [-861.232] (-864.680) * [-858.240] (-863.364) (-861.880) (-860.142) -- 0:02:57 107000 -- (-863.077) (-859.291) (-855.950) [-860.143] * (-860.630) [-858.691] (-858.323) (-863.605) -- 0:02:55 108000 -- (-862.270) (-856.644) [-868.557] (-858.393) * [-858.786] (-868.549) (-860.472) (-860.082) -- 0:03:01 109000 -- [-856.557] (-859.580) (-855.332) (-861.640) * (-859.850) (-858.892) [-855.260] (-859.891) -- 0:02:59 110000 -- (-862.168) (-858.166) [-861.445] (-857.212) * (-857.169) [-853.714] (-860.903) (-865.190) -- 0:02:58 Average standard deviation of split frequencies: 0.012451 111000 -- [-863.638] (-858.448) (-861.206) (-859.654) * (-861.666) (-860.020) [-855.693] (-857.791) -- 0:02:56 112000 -- (-866.587) [-857.268] (-865.021) (-855.674) * (-862.351) [-860.699] (-858.334) (-860.651) -- 0:02:54 113000 -- (-867.446) (-857.200) (-857.401) [-858.419] * [-855.265] (-856.238) (-869.463) (-864.443) -- 0:03:00 114000 -- (-856.748) [-857.904] (-859.354) (-856.487) * (-857.319) (-860.761) (-860.516) [-862.323] -- 0:02:58 115000 -- (-862.103) [-857.018] (-858.049) (-855.217) * (-854.727) [-860.157] (-863.899) (-867.296) -- 0:02:57 Average standard deviation of split frequencies: 0.015630 116000 -- (-873.432) (-855.664) (-856.813) [-864.202] * (-857.661) (-857.710) [-858.673] (-863.253) -- 0:02:55 117000 -- (-864.307) (-857.811) [-858.252] (-862.308) * [-856.418] (-859.985) (-861.330) (-858.220) -- 0:02:53 118000 -- (-859.514) [-854.416] (-854.815) (-862.099) * (-867.778) [-857.279] (-857.388) (-864.089) -- 0:02:59 119000 -- (-855.656) [-854.965] (-859.364) (-863.878) * [-859.065] (-857.078) (-863.491) (-873.520) -- 0:02:57 120000 -- (-857.858) (-870.533) [-859.866] (-862.729) * (-862.303) (-860.462) (-856.341) [-854.220] -- 0:02:56 Average standard deviation of split frequencies: 0.014425 121000 -- (-856.385) (-857.232) [-859.112] (-864.974) * [-859.306] (-862.692) (-860.635) (-860.555) -- 0:02:54 122000 -- [-856.819] (-869.231) (-855.964) (-864.448) * (-861.327) [-857.746] (-860.576) (-858.601) -- 0:02:52 123000 -- (-852.616) (-858.729) [-856.015] (-863.577) * (-861.320) (-854.183) [-859.605] (-856.028) -- 0:02:58 124000 -- [-857.397] (-860.806) (-864.218) (-864.760) * (-860.784) (-867.630) (-861.684) [-856.426] -- 0:02:56 125000 -- [-851.149] (-867.804) (-859.657) (-853.956) * (-863.124) (-866.090) (-867.076) [-858.197] -- 0:02:55 Average standard deviation of split frequencies: 0.014965 126000 -- (-863.527) [-861.003] (-857.859) (-873.104) * (-859.296) (-864.937) [-854.696] (-865.217) -- 0:02:53 127000 -- (-859.775) (-863.753) (-859.431) [-859.522] * (-855.272) (-869.718) [-863.631] (-863.717) -- 0:02:51 128000 -- (-857.369) [-862.469] (-858.447) (-865.269) * [-864.650] (-858.403) (-867.165) (-857.753) -- 0:02:57 129000 -- (-859.345) (-866.115) [-857.702] (-856.050) * (-864.959) (-857.482) (-860.426) [-859.161] -- 0:02:55 130000 -- [-857.923] (-871.802) (-860.710) (-867.127) * (-862.488) (-863.519) [-860.080] (-870.551) -- 0:02:54 Average standard deviation of split frequencies: 0.015818 131000 -- (-868.777) (-863.391) (-862.710) [-862.414] * [-864.011] (-865.882) (-856.386) (-870.192) -- 0:02:52 132000 -- [-862.595] (-858.179) (-859.878) (-865.020) * (-858.890) [-858.687] (-858.069) (-859.704) -- 0:02:50 133000 -- (-859.445) [-864.950] (-860.677) (-857.626) * (-859.699) [-858.756] (-857.095) (-857.061) -- 0:02:56 134000 -- (-860.379) (-859.007) [-856.255] (-863.099) * (-869.033) [-858.079] (-860.517) (-860.349) -- 0:02:54 135000 -- (-856.448) [-859.071] (-860.279) (-857.137) * [-857.659] (-863.074) (-864.807) (-859.519) -- 0:02:53 Average standard deviation of split frequencies: 0.016265 136000 -- (-871.243) (-859.791) [-853.581] (-863.740) * (-859.413) (-866.742) [-859.536] (-863.417) -- 0:02:51 137000 -- (-870.012) (-854.736) (-857.551) [-856.025] * (-859.203) [-861.159] (-854.239) (-866.192) -- 0:02:50 138000 -- (-859.741) [-859.423] (-863.410) (-867.435) * [-855.847] (-865.484) (-855.144) (-864.422) -- 0:02:54 139000 -- (-860.719) [-862.270] (-862.370) (-864.443) * (-856.205) [-853.132] (-862.869) (-863.933) -- 0:02:53 140000 -- (-856.749) (-857.290) (-865.251) [-861.613] * (-855.505) [-855.741] (-859.279) (-862.510) -- 0:02:52 Average standard deviation of split frequencies: 0.014178 141000 -- (-860.543) (-865.739) [-858.661] (-858.211) * (-856.660) [-857.046] (-857.947) (-858.373) -- 0:02:50 142000 -- (-863.449) (-856.341) (-863.286) [-854.853] * (-859.330) (-853.064) [-865.321] (-857.323) -- 0:02:49 143000 -- (-857.393) [-865.945] (-864.776) (-861.869) * (-863.563) (-862.187) [-864.669] (-866.247) -- 0:02:53 144000 -- (-861.531) [-860.742] (-856.735) (-863.290) * [-860.234] (-858.427) (-863.732) (-859.824) -- 0:02:52 145000 -- (-864.236) (-859.575) [-859.039] (-863.038) * (-858.054) [-859.396] (-861.958) (-863.312) -- 0:02:51 Average standard deviation of split frequencies: 0.015647 146000 -- (-863.194) (-855.394) [-856.735] (-859.492) * (-863.266) (-856.894) (-860.890) [-857.813] -- 0:02:49 147000 -- (-863.968) [-854.556] (-858.433) (-858.928) * (-855.842) (-865.929) [-851.782] (-856.314) -- 0:02:48 148000 -- [-859.887] (-859.729) (-857.299) (-858.896) * [-856.731] (-861.428) (-860.138) (-867.690) -- 0:02:52 149000 -- (-854.014) (-861.778) (-864.408) [-859.846] * (-861.338) (-861.093) (-870.742) [-856.142] -- 0:02:51 150000 -- [-867.039] (-858.282) (-860.597) (-862.747) * (-861.633) (-860.795) (-860.832) [-859.354] -- 0:02:50 Average standard deviation of split frequencies: 0.017569 151000 -- (-857.634) (-859.899) [-862.854] (-859.556) * (-859.558) (-864.876) (-863.721) [-859.184] -- 0:02:48 152000 -- [-861.839] (-860.680) (-862.481) (-862.055) * (-859.553) (-863.545) (-859.839) [-857.963] -- 0:02:47 153000 -- (-869.371) (-863.392) (-859.331) [-862.179] * [-864.625] (-860.762) (-863.159) (-854.261) -- 0:02:51 154000 -- (-868.273) (-863.149) (-856.304) [-862.501] * (-860.879) (-865.288) (-870.559) [-853.914] -- 0:02:50 155000 -- (-861.057) [-856.708] (-862.708) (-861.186) * (-859.615) (-866.961) (-859.831) [-858.543] -- 0:02:49 Average standard deviation of split frequencies: 0.017666 156000 -- [-861.472] (-856.406) (-858.939) (-855.011) * (-877.613) (-864.705) (-858.253) [-856.847] -- 0:02:47 157000 -- (-861.166) (-871.508) [-860.524] (-856.096) * (-873.908) (-864.690) (-855.802) [-862.426] -- 0:02:46 158000 -- (-861.483) [-855.232] (-861.845) (-868.159) * (-854.387) (-862.598) [-860.879] (-868.752) -- 0:02:50 159000 -- (-871.720) [-858.666] (-861.028) (-856.057) * (-858.206) (-856.060) (-857.174) [-860.147] -- 0:02:49 160000 -- [-864.309] (-867.912) (-864.782) (-859.685) * [-855.769] (-858.035) (-857.368) (-862.947) -- 0:02:48 Average standard deviation of split frequencies: 0.018733 161000 -- (-860.832) (-858.885) (-855.653) [-857.622] * [-860.595] (-857.252) (-856.429) (-862.995) -- 0:02:46 162000 -- (-861.031) (-857.482) (-863.847) [-862.507] * (-866.299) (-859.293) (-856.339) [-854.462] -- 0:02:45 163000 -- [-858.688] (-857.671) (-860.766) (-857.523) * (-863.751) (-857.970) (-858.654) [-858.648] -- 0:02:49 164000 -- (-863.744) [-855.571] (-862.594) (-864.536) * (-855.551) [-857.053] (-857.337) (-859.421) -- 0:02:48 165000 -- (-858.116) (-855.406) [-857.453] (-875.377) * (-866.366) (-859.919) (-854.283) [-857.005] -- 0:02:47 Average standard deviation of split frequencies: 0.016383 166000 -- [-859.512] (-856.845) (-860.365) (-864.955) * (-862.449) (-861.676) (-856.838) [-858.462] -- 0:02:45 167000 -- (-857.866) (-853.389) [-857.613] (-866.529) * (-855.489) [-857.698] (-861.375) (-861.444) -- 0:02:44 168000 -- [-859.302] (-855.831) (-855.359) (-866.365) * (-869.028) (-859.744) (-861.407) [-860.353] -- 0:02:43 169000 -- (-863.495) (-862.215) [-856.282] (-864.013) * (-860.280) [-856.451] (-860.914) (-861.130) -- 0:02:47 170000 -- (-855.958) (-861.175) [-861.639] (-856.736) * (-866.357) [-856.564] (-857.111) (-863.383) -- 0:02:46 Average standard deviation of split frequencies: 0.013173 171000 -- (-857.450) (-868.035) [-860.143] (-866.980) * (-859.843) (-865.044) [-858.193] (-857.226) -- 0:02:44 172000 -- (-866.888) (-872.037) [-852.151] (-863.366) * (-861.673) (-865.236) (-858.727) [-862.091] -- 0:02:43 173000 -- (-861.271) (-858.886) [-853.833] (-853.685) * (-869.624) (-856.824) [-857.262] (-854.759) -- 0:02:42 174000 -- (-859.613) (-856.622) (-859.044) [-862.756] * (-860.595) [-862.884] (-862.118) (-856.935) -- 0:02:46 175000 -- [-860.618] (-862.826) (-857.522) (-857.094) * (-862.403) (-853.199) (-861.668) [-854.561] -- 0:02:45 Average standard deviation of split frequencies: 0.013186 176000 -- (-866.016) [-856.741] (-856.307) (-860.782) * [-852.508] (-860.307) (-855.669) (-863.260) -- 0:02:43 177000 -- (-854.828) [-860.420] (-867.172) (-855.531) * (-858.017) [-855.686] (-867.062) (-859.833) -- 0:02:42 178000 -- [-861.808] (-866.500) (-853.670) (-863.521) * (-855.846) (-856.596) (-862.407) [-859.185] -- 0:02:41 179000 -- (-871.494) (-860.112) [-857.980] (-859.737) * (-861.624) (-860.860) [-862.415] (-857.856) -- 0:02:45 180000 -- [-862.951] (-857.477) (-860.050) (-854.777) * [-852.393] (-858.665) (-857.911) (-857.705) -- 0:02:44 Average standard deviation of split frequencies: 0.012043 181000 -- (-862.858) (-866.662) [-857.650] (-857.940) * (-857.027) (-858.875) (-856.134) [-861.632] -- 0:02:42 182000 -- (-860.063) [-856.171] (-863.803) (-856.803) * (-853.666) (-859.681) (-855.460) [-852.712] -- 0:02:41 183000 -- (-862.046) (-859.908) (-865.002) [-857.225] * (-862.753) (-860.178) (-858.267) [-856.061] -- 0:02:40 184000 -- (-861.538) [-863.226] (-860.784) (-860.092) * (-862.579) (-863.401) [-859.334] (-860.770) -- 0:02:44 185000 -- (-854.796) [-856.303] (-860.103) (-861.376) * (-861.747) (-860.460) [-865.440] (-854.848) -- 0:02:43 Average standard deviation of split frequencies: 0.013647 186000 -- [-856.524] (-857.660) (-855.663) (-860.966) * (-861.513) (-856.946) (-866.009) [-855.461] -- 0:02:41 187000 -- [-853.739] (-861.737) (-867.180) (-855.924) * (-858.926) (-858.230) (-858.046) [-859.830] -- 0:02:40 188000 -- [-854.301] (-860.950) (-864.555) (-866.546) * (-860.828) [-857.076] (-858.202) (-863.303) -- 0:02:39 189000 -- (-860.858) [-850.889] (-866.675) (-865.311) * (-856.520) (-857.899) [-858.675] (-877.546) -- 0:02:43 190000 -- (-861.790) (-852.303) [-859.269] (-860.654) * (-859.628) [-855.132] (-863.612) (-859.561) -- 0:02:42 Average standard deviation of split frequencies: 0.016166 191000 -- (-863.677) [-859.912] (-858.933) (-856.409) * (-864.542) [-860.363] (-857.777) (-852.663) -- 0:02:40 192000 -- (-864.562) (-855.558) (-862.001) [-859.758] * (-854.126) [-854.419] (-854.997) (-862.142) -- 0:02:39 193000 -- (-868.540) (-857.801) (-860.137) [-855.736] * (-856.525) [-856.529] (-862.011) (-861.065) -- 0:02:38 194000 -- (-856.425) (-860.184) [-864.508] (-855.209) * [-854.449] (-859.064) (-872.037) (-863.030) -- 0:02:42 195000 -- [-858.227] (-863.812) (-859.214) (-860.828) * [-856.782] (-860.628) (-852.399) (-862.461) -- 0:02:41 Average standard deviation of split frequencies: 0.015911 196000 -- [-857.754] (-865.001) (-866.286) (-863.707) * (-863.100) [-857.398] (-860.766) (-863.381) -- 0:02:39 197000 -- (-866.077) (-855.326) [-860.116] (-863.240) * (-857.874) [-854.833] (-866.261) (-863.895) -- 0:02:38 198000 -- (-869.016) (-854.230) (-857.373) [-856.638] * (-865.976) [-860.312] (-856.516) (-858.507) -- 0:02:37 199000 -- (-856.898) [-858.362] (-858.852) (-862.189) * (-861.620) [-852.782] (-857.528) (-861.727) -- 0:02:41 200000 -- (-859.535) [-857.616] (-856.635) (-861.659) * (-868.623) (-856.674) [-857.801] (-859.367) -- 0:02:40 Average standard deviation of split frequencies: 0.015541 201000 -- (-859.089) (-864.786) [-859.522] (-860.647) * (-865.939) (-859.549) (-865.514) [-863.094] -- 0:02:39 202000 -- [-859.216] (-858.802) (-858.099) (-854.833) * (-854.986) [-861.758] (-870.164) (-856.050) -- 0:02:38 203000 -- (-861.850) (-862.411) [-857.905] (-861.194) * (-854.869) [-861.952] (-856.587) (-856.427) -- 0:02:37 204000 -- (-863.086) [-853.363] (-868.851) (-858.420) * (-856.889) [-860.448] (-860.981) (-862.846) -- 0:02:39 205000 -- (-857.151) [-858.209] (-870.238) (-856.412) * (-857.866) (-858.005) [-856.251] (-857.977) -- 0:02:39 Average standard deviation of split frequencies: 0.015138 206000 -- (-860.941) (-861.002) (-864.651) [-855.455] * (-857.099) [-862.310] (-857.372) (-862.026) -- 0:02:38 207000 -- (-872.265) [-854.337] (-864.052) (-860.099) * (-861.001) [-860.440] (-852.009) (-863.686) -- 0:02:37 208000 -- (-876.638) (-856.494) [-853.053] (-859.550) * [-863.209] (-865.696) (-856.246) (-861.953) -- 0:02:36 209000 -- (-867.714) [-854.841] (-863.468) (-856.778) * (-861.444) [-864.350] (-856.532) (-862.520) -- 0:02:38 210000 -- (-864.344) (-858.082) (-862.518) [-862.661] * (-866.827) [-858.211] (-861.911) (-865.448) -- 0:02:38 Average standard deviation of split frequencies: 0.012393 211000 -- [-855.456] (-855.526) (-855.740) (-863.267) * (-867.714) (-866.395) (-856.369) [-859.173] -- 0:02:37 212000 -- (-859.379) (-863.545) [-858.481] (-866.867) * (-857.226) (-873.311) [-856.817] (-855.298) -- 0:02:36 213000 -- [-853.461] (-860.520) (-859.228) (-854.616) * (-861.719) (-860.408) (-855.497) [-854.039] -- 0:02:35 214000 -- (-856.175) [-858.033] (-865.823) (-857.446) * [-861.646] (-861.635) (-856.493) (-859.742) -- 0:02:37 215000 -- (-856.564) (-857.548) (-860.503) [-861.252] * [-858.394] (-867.157) (-861.026) (-855.676) -- 0:02:37 Average standard deviation of split frequencies: 0.012255 216000 -- (-857.232) [-856.398] (-859.296) (-855.941) * (-856.036) (-863.042) [-862.265] (-860.406) -- 0:02:36 217000 -- (-858.169) (-862.211) [-857.593] (-862.914) * [-858.506] (-857.676) (-855.911) (-856.902) -- 0:02:35 218000 -- [-863.656] (-860.108) (-857.145) (-861.491) * [-858.616] (-862.775) (-862.186) (-855.159) -- 0:02:34 219000 -- [-860.670] (-861.009) (-861.371) (-864.719) * (-864.407) (-862.421) (-863.451) [-857.624] -- 0:02:36 220000 -- (-863.312) [-867.899] (-862.413) (-869.187) * (-857.930) [-859.849] (-856.138) (-861.000) -- 0:02:36 Average standard deviation of split frequencies: 0.010353 221000 -- (-858.993) [-857.988] (-864.676) (-868.134) * (-857.240) (-860.529) (-862.133) [-863.617] -- 0:02:35 222000 -- (-860.834) (-855.210) (-864.453) [-862.470] * (-854.200) (-861.534) (-858.102) [-858.783] -- 0:02:34 223000 -- (-863.079) [-858.675] (-875.859) (-858.816) * (-856.844) [-857.485] (-853.460) (-858.007) -- 0:02:33 224000 -- (-861.312) [-861.556] (-862.994) (-859.989) * (-857.015) [-855.621] (-859.924) (-862.562) -- 0:02:35 225000 -- (-857.299) (-863.351) [-861.437] (-863.440) * (-859.712) (-858.698) (-865.275) [-856.065] -- 0:02:35 Average standard deviation of split frequencies: 0.008825 226000 -- (-858.805) [-860.190] (-859.584) (-858.424) * (-859.042) (-862.866) (-862.925) [-856.848] -- 0:02:34 227000 -- (-863.214) (-861.500) [-860.472] (-870.103) * [-860.020] (-858.952) (-867.556) (-865.909) -- 0:02:33 228000 -- (-867.409) (-858.559) (-858.066) [-857.455] * (-859.518) [-859.148] (-865.491) (-865.571) -- 0:02:32 229000 -- (-859.014) [-856.094] (-867.822) (-862.604) * [-857.098] (-871.249) (-863.403) (-858.730) -- 0:02:34 230000 -- (-861.346) [-856.037] (-858.628) (-857.884) * [-855.409] (-859.774) (-858.503) (-864.589) -- 0:02:34 Average standard deviation of split frequencies: 0.008646 231000 -- (-864.180) (-856.718) (-863.819) [-865.970] * [-862.524] (-862.934) (-860.207) (-860.895) -- 0:02:33 232000 -- (-865.096) [-857.714] (-856.799) (-858.251) * (-857.789) (-854.918) [-857.366] (-865.829) -- 0:02:32 233000 -- (-863.782) (-859.056) (-862.258) [-855.406] * (-858.467) (-861.729) [-861.416] (-863.527) -- 0:02:31 234000 -- [-858.889] (-854.225) (-859.078) (-861.799) * [-858.576] (-862.465) (-859.564) (-862.648) -- 0:02:33 235000 -- (-861.923) (-861.148) (-866.151) [-864.388] * (-856.917) [-861.082] (-865.862) (-859.908) -- 0:02:33 Average standard deviation of split frequencies: 0.008144 236000 -- (-864.014) [-857.388] (-862.023) (-863.010) * [-853.981] (-855.079) (-864.176) (-864.282) -- 0:02:32 237000 -- (-859.766) (-855.827) (-865.525) [-860.872] * (-862.937) [-861.941] (-866.970) (-856.557) -- 0:02:31 238000 -- [-867.584] (-859.415) (-862.209) (-863.815) * (-858.989) [-858.373] (-860.036) (-862.053) -- 0:02:30 239000 -- (-866.506) (-860.406) [-861.409] (-860.572) * [-854.942] (-858.009) (-859.422) (-867.162) -- 0:02:32 240000 -- (-875.995) [-858.209] (-862.547) (-860.149) * (-860.780) (-860.342) (-856.517) [-855.423] -- 0:02:32 Average standard deviation of split frequencies: 0.007684 241000 -- (-868.126) (-863.795) [-857.248] (-862.249) * (-862.170) (-859.673) (-858.630) [-854.255] -- 0:02:31 242000 -- (-866.868) (-862.608) [-860.775] (-862.802) * (-861.667) (-854.583) (-856.344) [-860.636] -- 0:02:30 243000 -- (-859.022) (-860.499) [-862.960] (-863.131) * (-865.149) (-861.889) [-856.172] (-861.102) -- 0:02:29 244000 -- (-862.608) (-862.470) [-857.595] (-858.724) * (-870.410) (-860.754) (-857.390) [-857.954] -- 0:02:31 245000 -- (-860.990) [-856.161] (-865.438) (-858.182) * (-862.818) (-860.712) [-856.854] (-856.532) -- 0:02:31 Average standard deviation of split frequencies: 0.007223 246000 -- (-866.933) [-854.458] (-860.208) (-865.309) * (-856.110) [-859.167] (-857.737) (-860.995) -- 0:02:30 247000 -- (-863.899) [-855.715] (-858.475) (-856.300) * (-862.031) [-852.570] (-858.431) (-858.348) -- 0:02:29 248000 -- (-870.936) [-857.251] (-858.038) (-863.454) * (-861.180) (-864.453) (-865.699) [-856.804] -- 0:02:28 249000 -- (-865.407) [-855.534] (-858.371) (-866.338) * (-861.339) [-863.190] (-860.341) (-858.744) -- 0:02:30 250000 -- (-860.485) (-860.139) (-862.085) [-858.136] * (-857.431) (-862.504) [-859.512] (-864.325) -- 0:02:30 Average standard deviation of split frequencies: 0.006944 251000 -- [-860.123] (-857.915) (-859.509) (-873.050) * (-865.235) (-861.405) (-859.082) [-862.046] -- 0:02:29 252000 -- [-862.651] (-850.184) (-863.198) (-864.525) * (-859.560) (-865.568) (-856.342) [-856.062] -- 0:02:28 253000 -- [-863.583] (-862.817) (-857.039) (-863.937) * (-853.992) [-862.851] (-859.193) (-867.203) -- 0:02:27 254000 -- (-856.931) (-861.500) [-857.041] (-863.263) * (-863.790) (-858.350) [-852.671] (-861.395) -- 0:02:29 255000 -- (-862.128) (-864.461) (-865.019) [-858.320] * (-869.925) (-856.758) [-853.759] (-856.953) -- 0:02:29 Average standard deviation of split frequencies: 0.008216 256000 -- (-858.600) (-869.586) [-857.677] (-870.068) * (-855.829) (-852.402) (-856.486) [-860.746] -- 0:02:28 257000 -- [-861.159] (-866.750) (-859.152) (-866.371) * [-857.951] (-854.107) (-860.916) (-860.424) -- 0:02:27 258000 -- (-859.078) (-854.990) (-856.793) [-857.921] * (-864.514) [-855.935] (-859.977) (-868.239) -- 0:02:26 259000 -- (-864.898) (-864.902) [-853.080] (-862.091) * (-860.182) [-855.329] (-858.612) (-858.558) -- 0:02:28 260000 -- (-863.748) (-853.610) (-858.534) [-858.548] * (-860.740) [-854.564] (-861.608) (-863.572) -- 0:02:28 Average standard deviation of split frequencies: 0.009181 261000 -- (-864.598) [-854.811] (-863.088) (-862.068) * [-858.521] (-861.817) (-863.612) (-863.462) -- 0:02:27 262000 -- [-857.100] (-858.429) (-862.878) (-870.036) * (-863.936) (-865.971) [-855.447] (-855.655) -- 0:02:26 263000 -- [-857.099] (-870.203) (-863.391) (-855.487) * [-861.090] (-856.207) (-854.919) (-862.457) -- 0:02:25 264000 -- [-860.022] (-856.820) (-864.015) (-872.222) * (-856.481) (-859.226) [-863.573] (-862.437) -- 0:02:27 265000 -- [-853.780] (-857.432) (-856.921) (-864.441) * [-858.571] (-864.154) (-863.969) (-861.400) -- 0:02:27 Average standard deviation of split frequencies: 0.009270 266000 -- (-864.986) (-861.815) [-857.976] (-860.610) * (-857.706) (-870.147) [-854.526] (-859.591) -- 0:02:26 267000 -- (-855.374) (-859.701) (-856.710) [-858.356] * (-854.897) (-865.581) (-873.387) [-855.012] -- 0:02:25 268000 -- [-853.696] (-864.257) (-859.134) (-859.800) * (-856.891) (-870.398) (-856.136) [-861.051] -- 0:02:24 269000 -- (-865.497) (-857.738) [-855.605] (-856.730) * (-857.760) [-860.324] (-861.111) (-855.141) -- 0:02:26 270000 -- (-858.033) [-860.714] (-859.464) (-864.443) * (-859.360) (-859.476) (-862.046) [-862.082] -- 0:02:26 Average standard deviation of split frequencies: 0.009914 271000 -- [-855.107] (-864.131) (-855.751) (-866.777) * [-860.201] (-858.320) (-863.858) (-861.909) -- 0:02:25 272000 -- (-858.970) [-853.610] (-859.893) (-856.651) * (-856.318) (-855.363) [-855.198] (-853.863) -- 0:02:24 273000 -- (-860.266) (-861.591) (-854.185) [-858.805] * (-863.355) (-856.456) (-858.515) [-852.659] -- 0:02:23 274000 -- (-857.450) (-857.887) (-859.574) [-853.026] * (-858.479) (-859.854) [-853.907] (-862.372) -- 0:02:25 275000 -- [-858.498] (-861.774) (-862.715) (-863.066) * (-860.130) (-860.935) [-861.038] (-862.350) -- 0:02:25 Average standard deviation of split frequencies: 0.008671 276000 -- (-862.676) (-859.740) (-867.060) [-858.893] * (-865.954) (-861.347) (-860.289) [-865.865] -- 0:02:24 277000 -- [-855.018] (-853.303) (-857.607) (-857.849) * (-861.464) (-869.801) (-857.977) [-855.917] -- 0:02:23 278000 -- [-856.241] (-861.217) (-867.582) (-866.064) * (-856.622) (-859.802) (-857.834) [-862.967] -- 0:02:22 279000 -- (-860.481) (-860.119) (-860.620) [-862.510] * (-859.598) (-856.860) (-855.142) [-858.128] -- 0:02:24 280000 -- (-856.234) [-853.156] (-859.593) (-857.884) * (-858.803) (-863.885) [-859.286] (-862.272) -- 0:02:24 Average standard deviation of split frequencies: 0.010078 281000 -- (-861.687) (-857.455) (-860.126) [-856.895] * (-862.129) [-863.323] (-855.856) (-854.012) -- 0:02:23 282000 -- (-859.469) [-857.643] (-858.571) (-854.555) * (-863.987) [-859.675] (-857.924) (-865.249) -- 0:02:22 283000 -- (-864.662) (-856.242) [-862.695] (-861.665) * (-856.844) (-859.947) (-861.868) [-859.360] -- 0:02:21 284000 -- [-863.050] (-854.922) (-859.149) (-858.166) * [-861.435] (-860.806) (-860.935) (-858.917) -- 0:02:23 285000 -- [-856.049] (-863.650) (-863.319) (-860.197) * (-866.507) (-855.165) (-862.507) [-854.222] -- 0:02:23 Average standard deviation of split frequencies: 0.011284 286000 -- [-857.872] (-862.044) (-856.764) (-859.996) * (-863.242) [-862.025] (-862.154) (-857.475) -- 0:02:22 287000 -- [-853.173] (-854.418) (-859.847) (-863.926) * [-857.657] (-858.335) (-858.588) (-858.555) -- 0:02:21 288000 -- [-859.117] (-868.480) (-859.941) (-864.352) * (-857.562) (-863.003) [-855.079] (-865.443) -- 0:02:20 289000 -- (-862.633) [-860.245] (-860.281) (-857.914) * [-855.117] (-861.643) (-856.719) (-856.886) -- 0:02:22 290000 -- (-860.392) [-854.173] (-866.825) (-863.193) * (-861.284) (-862.524) (-865.160) [-863.861] -- 0:02:22 Average standard deviation of split frequencies: 0.010105 291000 -- (-857.762) [-856.292] (-855.811) (-862.257) * (-862.647) (-863.984) (-856.693) [-861.075] -- 0:02:21 292000 -- (-859.152) [-858.651] (-864.282) (-866.703) * (-858.499) [-858.660] (-862.159) (-857.260) -- 0:02:20 293000 -- (-861.728) (-858.725) [-854.508] (-857.558) * (-861.491) (-860.793) (-860.503) [-854.410] -- 0:02:19 294000 -- (-855.557) [-857.946] (-859.703) (-864.309) * [-856.110] (-854.721) (-861.743) (-870.454) -- 0:02:21 295000 -- (-856.693) (-856.610) [-855.129] (-870.576) * (-858.789) (-854.985) [-861.021] (-857.432) -- 0:02:21 Average standard deviation of split frequencies: 0.008943 296000 -- (-856.436) (-856.284) [-863.082] (-862.382) * (-863.302) [-857.886] (-860.519) (-854.752) -- 0:02:20 297000 -- (-859.950) [-856.933] (-862.098) (-865.951) * (-865.000) (-854.909) (-863.986) [-857.884] -- 0:02:19 298000 -- (-862.293) [-856.742] (-859.598) (-856.041) * (-862.495) [-857.666] (-861.622) (-869.542) -- 0:02:18 299000 -- (-859.521) [-856.905] (-860.068) (-863.863) * (-863.448) (-860.182) [-860.456] (-855.569) -- 0:02:20 300000 -- [-859.962] (-860.440) (-873.239) (-859.339) * (-860.284) (-860.213) [-857.938] (-854.263) -- 0:02:20 Average standard deviation of split frequencies: 0.009769 301000 -- [-858.986] (-856.380) (-881.786) (-862.756) * (-859.632) (-857.970) [-857.971] (-858.711) -- 0:02:19 302000 -- (-858.962) (-863.508) [-860.926] (-872.422) * (-854.039) (-860.107) (-857.093) [-858.756] -- 0:02:18 303000 -- (-861.719) (-856.192) [-855.307] (-866.613) * (-863.343) [-862.319] (-861.238) (-861.099) -- 0:02:18 304000 -- (-872.284) (-865.403) [-863.926] (-868.709) * (-858.904) [-856.579] (-859.326) (-865.667) -- 0:02:19 305000 -- (-859.598) [-854.039] (-869.646) (-869.216) * [-858.888] (-862.526) (-855.976) (-864.794) -- 0:02:19 Average standard deviation of split frequencies: 0.008414 306000 -- (-861.498) [-862.494] (-866.630) (-861.989) * (-864.753) (-862.577) (-855.490) [-857.485] -- 0:02:18 307000 -- [-859.221] (-856.672) (-854.446) (-863.092) * (-857.548) [-862.339] (-857.258) (-858.965) -- 0:02:17 308000 -- (-858.267) [-857.837] (-858.804) (-858.357) * (-862.671) (-862.254) [-861.077] (-860.511) -- 0:02:17 309000 -- [-853.484] (-861.135) (-859.217) (-859.632) * (-866.014) (-858.955) [-860.987] (-855.251) -- 0:02:18 310000 -- (-859.668) (-855.139) [-861.129] (-859.712) * (-857.310) (-856.771) (-858.730) [-855.825] -- 0:02:18 Average standard deviation of split frequencies: 0.008287 311000 -- (-859.553) (-867.657) [-860.092] (-858.421) * [-855.812] (-862.234) (-857.137) (-864.937) -- 0:02:17 312000 -- [-857.310] (-856.486) (-858.839) (-863.276) * [-853.758] (-863.259) (-859.182) (-860.496) -- 0:02:16 313000 -- (-854.840) (-858.499) [-856.085] (-865.290) * [-853.289] (-858.152) (-854.165) (-865.997) -- 0:02:16 314000 -- [-860.535] (-862.553) (-860.357) (-869.277) * [-859.381] (-858.703) (-864.729) (-858.401) -- 0:02:17 315000 -- (-862.522) (-855.978) [-861.787] (-859.461) * (-861.896) (-858.411) (-857.569) [-860.519] -- 0:02:17 Average standard deviation of split frequencies: 0.007688 316000 -- (-859.765) (-858.233) (-862.603) [-859.736] * (-856.029) (-867.979) [-863.491] (-855.898) -- 0:02:16 317000 -- (-862.977) [-859.349] (-860.467) (-861.376) * (-863.908) (-858.006) [-854.569] (-861.979) -- 0:02:15 318000 -- (-862.820) (-855.686) (-859.330) [-862.953] * (-864.952) (-865.867) (-856.693) [-857.379] -- 0:02:15 319000 -- (-857.673) (-862.579) [-859.816] (-859.280) * [-858.622] (-854.622) (-857.485) (-857.685) -- 0:02:16 320000 -- [-857.947] (-862.125) (-859.066) (-860.852) * (-853.787) (-861.162) [-853.404] (-861.166) -- 0:02:16 Average standard deviation of split frequencies: 0.007350 321000 -- (-859.443) (-861.176) (-856.183) [-858.608] * (-853.302) (-869.336) [-861.789] (-863.187) -- 0:02:15 322000 -- (-859.573) (-857.770) (-861.193) [-860.674] * (-861.092) [-859.061] (-855.356) (-864.384) -- 0:02:14 323000 -- (-861.475) (-869.443) [-857.149] (-860.950) * [-856.016] (-860.450) (-856.506) (-863.413) -- 0:02:14 324000 -- (-857.714) (-862.037) [-859.790] (-858.728) * [-860.473] (-861.284) (-856.989) (-864.294) -- 0:02:15 325000 -- (-857.806) (-861.263) (-857.801) [-853.946] * (-870.068) (-854.713) (-866.111) [-860.342] -- 0:02:15 Average standard deviation of split frequencies: 0.007230 326000 -- (-867.564) [-862.838] (-856.799) (-856.511) * [-872.136] (-857.087) (-863.280) (-858.891) -- 0:02:14 327000 -- (-853.394) [-857.557] (-859.326) (-855.526) * (-862.287) [-860.690] (-860.911) (-858.635) -- 0:02:13 328000 -- (-862.968) [-856.735] (-857.047) (-857.577) * (-862.334) (-867.594) (-860.909) [-858.077] -- 0:02:13 329000 -- (-866.027) [-855.971] (-863.862) (-857.734) * [-858.837] (-859.997) (-864.259) (-853.782) -- 0:02:14 330000 -- (-864.319) [-858.779] (-865.544) (-857.399) * (-861.397) [-864.252] (-864.255) (-856.313) -- 0:02:14 Average standard deviation of split frequencies: 0.006909 331000 -- (-862.170) [-855.803] (-862.925) (-860.916) * (-853.776) (-859.043) (-862.680) [-857.496] -- 0:02:13 332000 -- (-859.546) (-859.909) (-861.828) [-856.780] * [-862.891] (-859.831) (-860.437) (-865.399) -- 0:02:12 333000 -- (-867.709) [-858.235] (-862.489) (-860.503) * (-862.528) (-861.657) (-862.621) [-859.418] -- 0:02:12 334000 -- [-860.681] (-865.695) (-864.324) (-857.060) * [-861.419] (-862.357) (-867.897) (-858.077) -- 0:02:13 335000 -- (-862.941) [-856.242] (-864.545) (-857.981) * [-856.034] (-858.028) (-863.494) (-855.906) -- 0:02:13 Average standard deviation of split frequencies: 0.006583 336000 -- (-862.204) [-857.601] (-857.484) (-857.679) * [-861.802] (-859.078) (-862.495) (-856.367) -- 0:02:12 337000 -- [-859.504] (-856.507) (-861.832) (-859.695) * (-859.178) (-864.206) [-862.444] (-857.829) -- 0:02:11 338000 -- [-854.681] (-859.377) (-868.617) (-858.051) * (-856.962) [-861.238] (-863.437) (-861.219) -- 0:02:11 339000 -- (-859.320) (-864.837) (-864.560) [-854.165] * (-854.462) (-865.926) [-863.252] (-857.345) -- 0:02:12 340000 -- [-859.429] (-859.272) (-864.880) (-853.706) * (-863.506) (-858.816) (-858.213) [-855.878] -- 0:02:12 Average standard deviation of split frequencies: 0.006493 341000 -- (-865.686) (-855.704) (-864.592) [-857.790] * (-857.851) (-864.981) (-864.163) [-857.690] -- 0:02:11 342000 -- (-855.439) (-865.595) (-863.739) [-857.108] * (-858.508) [-859.831] (-862.790) (-853.678) -- 0:02:10 343000 -- (-859.383) (-863.842) (-855.515) [-857.296] * [-859.841] (-858.137) (-855.076) (-857.683) -- 0:02:10 344000 -- (-862.417) (-860.069) [-856.374] (-858.523) * (-854.616) (-861.975) (-862.590) [-853.936] -- 0:02:11 345000 -- (-865.923) (-864.317) [-852.886] (-862.397) * (-861.011) (-859.631) (-864.732) [-855.286] -- 0:02:11 Average standard deviation of split frequencies: 0.006393 346000 -- (-863.433) (-862.510) (-856.083) [-860.558] * [-857.507] (-859.971) (-855.552) (-861.970) -- 0:02:10 347000 -- (-857.897) (-862.617) [-860.226] (-861.397) * (-860.484) (-859.183) [-858.983] (-856.773) -- 0:02:09 348000 -- (-864.570) [-855.582] (-863.007) (-857.805) * (-860.429) (-867.876) [-855.175] (-860.712) -- 0:02:09 349000 -- (-867.267) (-864.599) (-856.168) [-857.511] * (-862.859) [-855.206] (-864.187) (-861.106) -- 0:02:10 350000 -- (-855.799) (-860.934) (-855.721) [-860.495] * (-866.810) (-860.914) [-854.464] (-852.411) -- 0:02:10 Average standard deviation of split frequencies: 0.006618 351000 -- [-855.740] (-856.558) (-867.606) (-861.494) * (-867.187) [-853.452] (-858.817) (-858.324) -- 0:02:09 352000 -- (-858.284) (-864.533) (-854.630) [-861.021] * (-859.123) [-860.027] (-859.982) (-856.504) -- 0:02:08 353000 -- (-860.211) [-857.756] (-856.844) (-861.717) * (-857.004) [-862.178] (-858.767) (-859.886) -- 0:02:08 354000 -- (-864.044) (-867.252) (-855.288) [-858.570] * (-859.753) [-864.545] (-865.763) (-856.773) -- 0:02:09 355000 -- (-855.197) [-860.997] (-859.865) (-860.472) * (-857.215) (-864.727) (-861.383) [-855.187] -- 0:02:09 Average standard deviation of split frequencies: 0.007028 356000 -- [-859.578] (-861.448) (-864.325) (-858.000) * (-861.652) (-862.479) [-857.227] (-862.554) -- 0:02:08 357000 -- (-859.856) (-862.312) [-857.889] (-862.860) * (-860.025) [-864.634] (-856.325) (-869.969) -- 0:02:07 358000 -- (-856.483) [-860.894] (-857.470) (-857.580) * (-863.875) [-856.118] (-866.538) (-863.383) -- 0:02:07 359000 -- [-860.286] (-858.555) (-862.199) (-869.037) * [-864.597] (-862.424) (-857.466) (-863.792) -- 0:02:08 360000 -- (-856.967) [-860.250] (-860.190) (-864.849) * (-858.690) [-855.373] (-859.664) (-863.979) -- 0:02:08 Average standard deviation of split frequencies: 0.007541 361000 -- (-854.030) (-857.569) (-861.435) [-865.399] * (-865.523) (-859.895) [-859.280] (-861.091) -- 0:02:07 362000 -- (-857.706) (-861.937) (-860.188) [-859.403] * (-859.184) [-857.580] (-855.576) (-881.449) -- 0:02:06 363000 -- (-866.307) (-862.034) (-859.003) [-859.336] * (-853.493) (-859.940) [-860.745] (-860.380) -- 0:02:06 364000 -- (-861.036) (-864.749) [-867.672] (-853.320) * (-852.399) [-854.054] (-859.762) (-859.921) -- 0:02:07 365000 -- (-862.663) (-857.293) (-862.682) [-851.636] * (-858.368) (-865.608) [-857.983] (-862.089) -- 0:02:07 Average standard deviation of split frequencies: 0.008421 366000 -- (-867.576) (-861.476) (-857.414) [-855.631] * (-861.974) (-865.413) (-866.953) [-855.506] -- 0:02:06 367000 -- [-860.541] (-863.998) (-857.050) (-865.482) * (-855.299) [-857.816] (-857.683) (-859.031) -- 0:02:05 368000 -- (-861.211) (-861.244) (-864.645) [-858.312] * (-864.482) (-856.571) [-857.378] (-853.869) -- 0:02:05 369000 -- [-856.850] (-866.305) (-858.529) (-856.825) * (-859.482) (-856.600) (-857.944) [-856.855] -- 0:02:06 370000 -- (-859.698) [-863.558] (-861.959) (-860.790) * (-860.371) (-858.697) (-867.376) [-857.170] -- 0:02:06 Average standard deviation of split frequencies: 0.007337 371000 -- (-854.849) (-863.828) [-855.221] (-858.207) * (-863.049) [-854.370] (-861.519) (-857.672) -- 0:02:05 372000 -- [-858.891] (-859.205) (-860.993) (-861.868) * [-855.474] (-859.195) (-859.011) (-858.834) -- 0:02:04 373000 -- (-857.147) (-860.447) (-864.685) [-862.231] * (-857.344) (-860.653) (-865.645) [-858.811] -- 0:02:04 374000 -- (-863.114) (-862.580) [-858.110] (-859.035) * [-856.626] (-861.505) (-860.461) (-858.558) -- 0:02:05 375000 -- (-856.526) (-857.925) (-863.372) [-853.714] * [-860.766] (-860.725) (-856.298) (-858.994) -- 0:02:05 Average standard deviation of split frequencies: 0.007619 376000 -- (-859.616) (-862.521) (-861.453) [-856.456] * [-857.752] (-858.188) (-859.867) (-861.674) -- 0:02:04 377000 -- (-864.543) [-852.599] (-865.107) (-861.628) * (-868.783) (-866.643) [-859.079] (-863.284) -- 0:02:03 378000 -- (-863.641) (-859.453) [-856.878] (-859.404) * (-855.300) (-858.679) [-859.592] (-863.193) -- 0:02:03 379000 -- (-863.825) (-866.140) (-862.031) [-856.924] * [-857.433] (-856.775) (-858.842) (-856.885) -- 0:02:04 380000 -- (-858.382) (-857.252) (-866.943) [-859.218] * (-855.756) (-861.074) (-855.868) [-858.096] -- 0:02:04 Average standard deviation of split frequencies: 0.007906 381000 -- (-857.772) (-861.939) (-873.427) [-854.095] * [-859.965] (-859.898) (-860.435) (-855.866) -- 0:02:03 382000 -- (-863.935) (-859.982) (-865.397) [-861.734] * [-860.881] (-850.703) (-856.166) (-855.195) -- 0:02:02 383000 -- (-857.982) (-859.575) [-856.282] (-865.899) * (-861.150) [-863.007] (-857.438) (-858.472) -- 0:02:04 384000 -- (-860.225) (-866.714) (-870.332) [-857.433] * [-859.481] (-859.365) (-861.406) (-855.857) -- 0:02:03 385000 -- [-856.777] (-858.590) (-864.791) (-865.935) * [-855.561] (-858.645) (-862.589) (-860.394) -- 0:02:03 Average standard deviation of split frequencies: 0.008925 386000 -- (-857.311) (-861.195) [-858.329] (-858.111) * (-853.316) [-857.030] (-873.368) (-862.383) -- 0:02:02 387000 -- (-856.277) (-856.820) [-866.104] (-858.028) * (-863.366) [-865.282] (-859.652) (-864.458) -- 0:02:01 388000 -- (-859.295) [-859.403] (-867.473) (-853.560) * (-868.121) (-862.766) [-861.795] (-861.660) -- 0:02:03 389000 -- [-855.631] (-854.765) (-858.521) (-856.387) * (-859.413) (-857.659) (-870.066) [-858.454] -- 0:02:02 390000 -- (-857.329) (-857.761) [-862.315] (-864.758) * (-860.059) (-863.224) [-866.217] (-859.487) -- 0:02:02 Average standard deviation of split frequencies: 0.009189 391000 -- (-864.055) [-859.405] (-856.180) (-857.176) * (-856.063) (-871.864) (-856.889) [-859.256] -- 0:02:01 392000 -- (-864.956) (-860.499) [-857.603] (-860.167) * [-858.663] (-867.126) (-859.984) (-863.534) -- 0:02:00 393000 -- (-859.337) (-864.741) [-859.059] (-863.350) * (-859.201) (-861.847) (-866.565) [-857.851] -- 0:02:02 394000 -- (-866.328) (-864.264) (-862.425) [-857.094] * (-864.902) (-861.305) (-861.624) [-857.706] -- 0:02:01 395000 -- (-859.742) (-859.512) (-862.315) [-860.209] * (-862.062) (-868.981) (-860.628) [-859.470] -- 0:02:01 Average standard deviation of split frequencies: 0.009432 396000 -- (-861.132) [-856.737] (-855.702) (-859.519) * (-855.494) (-860.115) (-859.850) [-859.035] -- 0:02:00 397000 -- (-864.740) (-863.409) (-856.394) [-860.236] * (-857.954) (-860.445) [-860.441] (-865.981) -- 0:01:59 398000 -- (-866.464) (-871.636) [-859.737] (-861.326) * [-853.135] (-854.855) (-871.065) (-860.973) -- 0:01:59 399000 -- [-858.991] (-870.627) (-856.591) (-866.774) * (-855.752) (-860.993) [-859.311] (-870.911) -- 0:02:00 400000 -- (-867.879) (-856.446) [-853.218] (-861.812) * (-859.278) (-858.500) (-862.629) [-865.191] -- 0:02:00 Average standard deviation of split frequencies: 0.007874 401000 -- (-865.008) (-855.520) (-864.831) [-864.500] * [-860.021] (-859.247) (-860.727) (-868.474) -- 0:01:59 402000 -- (-865.224) (-858.725) (-862.822) [-857.656] * (-858.172) [-856.127] (-861.058) (-864.135) -- 0:01:59 403000 -- (-864.624) (-856.769) [-862.010] (-855.235) * (-857.039) [-855.240] (-858.507) (-858.341) -- 0:01:58 404000 -- [-856.456] (-854.714) (-865.142) (-855.564) * [-863.036] (-871.519) (-854.617) (-864.548) -- 0:01:59 405000 -- (-866.209) (-865.282) [-854.558] (-858.854) * [-856.947] (-859.816) (-859.611) (-860.876) -- 0:01:59 Average standard deviation of split frequencies: 0.007770 406000 -- [-853.859] (-855.017) (-861.809) (-856.978) * [-860.207] (-872.526) (-857.218) (-853.110) -- 0:01:58 407000 -- (-859.708) (-859.606) [-861.222] (-859.978) * (-861.562) (-864.397) [-862.456] (-864.749) -- 0:01:58 408000 -- (-858.048) (-861.254) (-861.869) [-863.573] * (-863.253) [-854.193] (-855.462) (-864.735) -- 0:01:58 409000 -- [-861.855] (-864.088) (-861.589) (-857.985) * [-864.833] (-859.053) (-859.124) (-864.966) -- 0:01:58 410000 -- [-860.813] (-854.889) (-859.084) (-867.873) * (-859.888) (-856.883) [-859.486] (-864.957) -- 0:01:58 Average standard deviation of split frequencies: 0.007594 411000 -- (-862.306) (-859.552) (-857.771) [-855.222] * (-858.245) (-854.332) [-855.391] (-862.737) -- 0:01:57 412000 -- (-855.318) (-861.514) (-867.239) [-857.960] * [-861.143] (-863.968) (-854.607) (-859.779) -- 0:01:57 413000 -- (-856.759) (-862.033) (-866.118) [-851.467] * (-860.794) [-858.578] (-856.096) (-860.016) -- 0:01:57 414000 -- (-866.010) [-861.549] (-865.597) (-861.492) * (-865.364) [-854.833] (-874.721) (-862.252) -- 0:01:57 415000 -- [-868.501] (-858.163) (-856.362) (-864.698) * (-863.574) (-857.139) [-868.327] (-857.690) -- 0:01:57 Average standard deviation of split frequencies: 0.007148 416000 -- [-852.935] (-863.270) (-862.004) (-862.106) * [-858.321] (-854.277) (-862.332) (-859.989) -- 0:01:56 417000 -- (-858.231) [-862.102] (-856.674) (-861.594) * (-858.209) [-856.016] (-858.705) (-856.032) -- 0:01:56 418000 -- (-857.229) [-860.672] (-867.585) (-854.455) * (-858.698) (-854.394) [-858.228] (-861.753) -- 0:01:56 419000 -- [-854.219] (-857.403) (-862.195) (-864.613) * [-856.053] (-857.289) (-870.375) (-863.887) -- 0:01:56 420000 -- (-863.105) (-860.540) (-869.133) [-853.061] * (-860.001) (-863.259) (-858.587) [-855.341] -- 0:01:56 Average standard deviation of split frequencies: 0.007241 421000 -- [-859.458] (-865.283) (-862.794) (-857.508) * (-859.713) (-864.317) (-852.996) [-856.169] -- 0:01:55 422000 -- [-860.326] (-864.121) (-872.384) (-860.854) * (-861.116) [-857.850] (-865.510) (-858.011) -- 0:01:55 423000 -- (-857.156) [-859.222] (-865.589) (-857.063) * (-863.202) [-855.520] (-862.148) (-863.189) -- 0:01:55 424000 -- (-856.888) (-859.114) (-866.371) [-860.037] * (-865.900) [-856.459] (-858.470) (-861.618) -- 0:01:55 425000 -- (-861.642) [-855.112] (-862.826) (-858.568) * (-864.620) (-858.970) [-853.296] (-863.316) -- 0:01:55 Average standard deviation of split frequencies: 0.006469 426000 -- (-868.322) [-857.154] (-864.754) (-859.713) * (-867.161) (-864.480) (-857.457) [-858.761] -- 0:01:54 427000 -- (-862.548) (-860.240) (-859.507) [-852.255] * [-859.576] (-866.732) (-859.100) (-856.696) -- 0:01:54 428000 -- (-855.932) [-862.789] (-860.263) (-858.099) * (-863.220) [-857.903] (-856.689) (-861.761) -- 0:01:54 429000 -- (-857.325) (-868.528) [-857.322] (-857.087) * (-860.422) (-856.727) (-861.837) [-866.281] -- 0:01:54 430000 -- (-856.098) [-854.702] (-866.995) (-863.145) * (-864.012) (-858.488) [-858.101] (-862.409) -- 0:01:54 Average standard deviation of split frequencies: 0.006062 431000 -- (-863.446) (-863.309) (-857.749) [-865.023] * (-860.902) (-857.432) (-863.435) [-857.127] -- 0:01:53 432000 -- (-860.456) [-856.082] (-860.744) (-854.828) * (-856.973) (-861.136) (-861.949) [-864.430] -- 0:01:53 433000 -- (-858.588) (-866.746) [-855.767] (-860.339) * [-862.896] (-858.084) (-869.051) (-854.897) -- 0:01:53 434000 -- (-867.603) (-859.555) (-859.596) [-852.403] * (-861.895) [-856.642] (-869.205) (-859.281) -- 0:01:53 435000 -- (-861.319) [-859.390] (-857.780) (-867.974) * (-865.959) (-860.064) (-865.068) [-858.742] -- 0:01:53 Average standard deviation of split frequencies: 0.005822 436000 -- (-867.135) [-854.562] (-863.105) (-862.385) * [-857.235] (-858.162) (-856.189) (-872.213) -- 0:01:52 437000 -- (-861.065) (-876.258) [-855.891] (-866.078) * (-864.062) [-853.642] (-861.482) (-855.169) -- 0:01:52 438000 -- (-856.349) (-860.507) [-855.052] (-860.202) * (-865.122) (-857.893) (-866.250) [-855.739] -- 0:01:52 439000 -- (-857.140) [-856.994] (-860.219) (-860.970) * (-869.982) [-855.080] (-862.873) (-857.836) -- 0:01:52 440000 -- (-862.824) [-862.807] (-869.059) (-855.035) * (-855.367) [-861.976] (-869.830) (-862.405) -- 0:01:52 Average standard deviation of split frequencies: 0.005431 441000 -- [-860.410] (-860.930) (-860.455) (-856.087) * [-855.629] (-863.358) (-862.047) (-857.956) -- 0:01:51 442000 -- (-869.313) (-860.443) [-860.651] (-858.064) * (-857.926) [-865.345] (-859.629) (-859.102) -- 0:01:51 443000 -- [-861.326] (-862.377) (-853.903) (-859.235) * (-855.028) (-857.718) (-858.935) [-859.946] -- 0:01:51 444000 -- (-860.633) [-859.244] (-863.516) (-855.849) * (-862.717) (-860.024) [-858.994] (-862.510) -- 0:01:51 445000 -- [-865.554] (-862.164) (-859.679) (-856.579) * [-855.409] (-858.534) (-863.097) (-856.900) -- 0:01:51 Average standard deviation of split frequencies: 0.005854 446000 -- (-857.624) (-860.999) (-867.220) [-858.252] * (-863.911) [-861.002] (-869.041) (-863.422) -- 0:01:50 447000 -- (-859.861) (-863.211) (-855.753) [-863.826] * (-858.046) (-861.789) (-859.667) [-861.780] -- 0:01:50 448000 -- (-859.326) [-853.682] (-863.891) (-853.655) * (-862.460) (-857.003) [-857.739] (-859.853) -- 0:01:50 449000 -- [-851.634] (-859.275) (-858.429) (-857.693) * (-858.028) (-863.692) (-862.750) [-863.603] -- 0:01:50 450000 -- (-857.474) (-865.337) [-862.169] (-856.007) * [-854.507] (-860.840) (-859.739) (-857.022) -- 0:01:50 Average standard deviation of split frequencies: 0.005471 451000 -- (-860.845) (-854.733) (-856.857) [-860.370] * (-856.188) (-859.432) [-866.310] (-862.004) -- 0:01:49 452000 -- (-858.045) [-859.343] (-863.047) (-858.971) * (-854.436) (-857.584) (-854.954) [-859.386] -- 0:01:49 453000 -- (-856.090) [-865.160] (-858.105) (-862.978) * [-856.358] (-855.348) (-859.101) (-863.802) -- 0:01:49 454000 -- (-858.873) (-855.411) (-852.270) [-854.207] * (-865.874) (-854.845) (-857.131) [-858.924] -- 0:01:49 455000 -- (-855.890) (-864.561) (-871.566) [-861.062] * [-855.249] (-855.321) (-859.302) (-858.072) -- 0:01:49 Average standard deviation of split frequencies: 0.006203 456000 -- (-856.820) [-855.393] (-860.566) (-861.852) * (-857.489) [-860.470] (-858.973) (-852.323) -- 0:01:48 457000 -- (-864.650) [-858.035] (-859.725) (-862.056) * (-857.845) [-855.607] (-863.587) (-859.978) -- 0:01:48 458000 -- (-858.422) (-855.333) [-866.039] (-864.007) * (-855.398) (-857.448) [-856.653] (-860.720) -- 0:01:48 459000 -- (-861.267) (-855.518) (-855.564) [-861.893] * (-853.262) (-860.709) [-858.904] (-861.489) -- 0:01:48 460000 -- (-857.979) (-864.850) (-858.429) [-857.096] * [-855.149] (-857.530) (-857.142) (-860.604) -- 0:01:48 Average standard deviation of split frequencies: 0.005510 461000 -- [-855.687] (-853.627) (-856.040) (-864.914) * (-857.998) (-857.749) [-857.976] (-866.864) -- 0:01:47 462000 -- (-859.237) (-860.094) [-871.075] (-862.094) * (-859.913) [-856.122] (-861.625) (-859.392) -- 0:01:47 463000 -- (-859.736) (-860.830) [-855.484] (-855.398) * (-859.495) [-861.473] (-862.356) (-853.191) -- 0:01:47 464000 -- (-857.188) (-855.809) [-858.679] (-855.511) * (-860.579) (-854.362) (-859.125) [-856.478] -- 0:01:47 465000 -- (-863.504) (-863.205) [-865.550] (-854.643) * [-853.327] (-867.320) (-862.734) (-853.193) -- 0:01:47 Average standard deviation of split frequencies: 0.006459 466000 -- [-860.568] (-853.350) (-858.379) (-864.094) * [-863.314] (-858.924) (-856.995) (-861.700) -- 0:01:46 467000 -- (-860.362) (-854.082) (-861.526) [-861.885] * (-855.048) (-870.769) (-862.683) [-856.250] -- 0:01:46 468000 -- (-858.331) [-858.837] (-859.015) (-862.487) * (-863.020) (-859.133) [-855.848] (-857.659) -- 0:01:46 469000 -- (-860.633) (-859.747) (-858.821) [-855.788] * [-858.909] (-861.542) (-861.031) (-859.212) -- 0:01:46 470000 -- (-855.412) (-862.263) (-860.098) [-851.799] * (-862.166) (-868.515) (-857.196) [-859.238] -- 0:01:46 Average standard deviation of split frequencies: 0.005624 471000 -- (-858.800) (-858.673) (-864.247) [-853.779] * [-854.782] (-862.674) (-860.407) (-854.774) -- 0:01:45 472000 -- [-855.241] (-868.281) (-859.431) (-869.421) * [-857.862] (-862.143) (-867.467) (-857.123) -- 0:01:45 473000 -- (-857.702) (-863.189) [-857.857] (-862.635) * (-860.967) (-858.048) [-860.975] (-857.818) -- 0:01:45 474000 -- (-852.794) (-858.334) (-858.518) [-857.454] * [-856.452] (-865.006) (-862.983) (-857.396) -- 0:01:45 475000 -- (-862.911) (-865.231) [-858.529] (-858.308) * (-860.878) [-856.111] (-867.086) (-857.918) -- 0:01:45 Average standard deviation of split frequencies: 0.006018 476000 -- (-865.463) [-858.645] (-866.504) (-862.486) * (-857.590) (-852.601) [-860.221] (-870.942) -- 0:01:44 477000 -- [-863.067] (-862.090) (-867.903) (-859.704) * (-863.199) (-859.743) (-857.521) [-862.803] -- 0:01:44 478000 -- (-857.008) (-866.344) [-863.335] (-862.837) * (-858.709) (-862.635) (-857.000) [-854.413] -- 0:01:44 479000 -- (-860.134) (-869.225) (-864.063) [-861.098] * (-861.082) (-866.211) [-858.551] (-860.744) -- 0:01:44 480000 -- (-855.263) (-867.155) [-860.501] (-859.898) * (-860.305) [-859.766] (-858.144) (-861.892) -- 0:01:44 Average standard deviation of split frequencies: 0.005507 481000 -- (-858.860) (-862.226) (-861.851) [-862.206] * (-860.153) (-864.716) [-856.234] (-856.802) -- 0:01:43 482000 -- (-854.981) (-865.116) [-855.996] (-856.225) * [-858.660] (-866.398) (-867.201) (-857.207) -- 0:01:43 483000 -- (-865.585) (-856.605) [-852.523] (-867.648) * [-855.343] (-863.170) (-862.577) (-857.524) -- 0:01:43 484000 -- (-857.301) (-859.273) [-860.003] (-857.011) * [-859.592] (-869.867) (-862.364) (-857.911) -- 0:01:43 485000 -- [-857.179] (-864.138) (-861.160) (-870.644) * (-862.777) [-862.458] (-859.084) (-859.339) -- 0:01:43 Average standard deviation of split frequencies: 0.005596 486000 -- [-860.683] (-861.926) (-863.171) (-858.332) * (-853.843) (-857.736) [-867.703] (-872.974) -- 0:01:42 487000 -- (-871.860) (-865.046) [-860.990] (-855.869) * (-863.495) (-854.559) (-855.998) [-857.220] -- 0:01:42 488000 -- (-867.457) (-864.019) (-857.911) [-862.603] * (-855.450) (-860.426) (-858.283) [-854.903] -- 0:01:42 489000 -- [-855.110] (-863.848) (-860.094) (-861.302) * (-856.565) (-863.402) (-861.209) [-861.889] -- 0:01:42 490000 -- (-861.619) (-858.303) (-860.626) [-856.514] * [-863.531] (-858.164) (-866.691) (-857.702) -- 0:01:42 Average standard deviation of split frequencies: 0.005247 491000 -- (-863.703) (-858.578) (-860.040) [-855.391] * [-862.678] (-856.846) (-862.232) (-860.488) -- 0:01:41 492000 -- (-858.370) [-860.984] (-857.523) (-856.002) * (-859.092) [-855.812] (-863.346) (-860.642) -- 0:01:41 493000 -- (-856.136) [-855.474] (-859.103) (-863.066) * (-859.568) [-855.341] (-862.691) (-859.573) -- 0:01:41 494000 -- (-855.800) [-857.328] (-867.527) (-859.726) * (-856.945) (-865.100) (-857.047) [-855.534] -- 0:01:41 495000 -- (-867.435) (-860.776) (-872.505) [-859.862] * [-853.369] (-858.253) (-856.497) (-858.413) -- 0:01:41 Average standard deviation of split frequencies: 0.006214 496000 -- (-870.049) [-860.074] (-860.446) (-863.200) * (-861.760) [-856.405] (-859.919) (-857.183) -- 0:01:40 497000 -- (-858.095) [-861.427] (-860.681) (-863.877) * (-860.234) [-866.351] (-858.086) (-862.277) -- 0:01:40 498000 -- (-862.409) [-858.118] (-859.406) (-856.747) * (-861.357) (-865.842) [-857.005] (-860.035) -- 0:01:40 499000 -- [-862.140] (-871.083) (-858.599) (-866.186) * (-862.154) (-861.319) (-864.673) [-863.553] -- 0:01:40 500000 -- (-854.433) (-861.632) (-859.262) [-859.004] * [-857.628] (-856.940) (-859.060) (-856.120) -- 0:01:40 Average standard deviation of split frequencies: 0.006736 501000 -- [-853.983] (-865.390) (-860.047) (-868.626) * (-865.009) (-862.677) (-855.845) [-857.674] -- 0:01:39 502000 -- (-855.954) (-852.834) [-866.786] (-858.916) * (-859.797) (-862.066) [-857.476] (-857.797) -- 0:01:39 503000 -- [-862.407] (-858.926) (-866.952) (-853.731) * (-857.900) [-861.992] (-860.861) (-863.057) -- 0:01:38 504000 -- (-862.253) (-863.300) (-857.958) [-860.579] * (-858.570) (-858.202) [-863.684] (-855.003) -- 0:01:39 505000 -- [-866.203] (-868.943) (-861.523) (-859.975) * (-863.318) [-854.213] (-864.039) (-858.418) -- 0:01:39 Average standard deviation of split frequencies: 0.006808 506000 -- [-856.843] (-857.868) (-862.513) (-855.501) * [-856.524] (-860.186) (-862.883) (-857.672) -- 0:01:38 507000 -- (-858.307) [-856.904] (-860.119) (-856.984) * [-862.787] (-855.043) (-866.422) (-860.908) -- 0:01:38 508000 -- (-860.864) (-855.639) (-857.533) [-860.137] * (-860.516) [-856.968] (-863.102) (-859.291) -- 0:01:37 509000 -- (-859.993) [-853.599] (-856.831) (-864.358) * (-858.074) (-862.244) (-861.716) [-862.979] -- 0:01:38 510000 -- (-859.518) [-857.080] (-861.479) (-860.411) * (-861.918) (-860.204) [-858.181] (-868.922) -- 0:01:38 Average standard deviation of split frequencies: 0.007456 511000 -- (-859.020) (-854.463) (-861.981) [-854.460] * (-864.794) (-864.995) [-859.666] (-864.967) -- 0:01:37 512000 -- (-867.715) (-862.523) [-858.927] (-860.073) * (-863.375) [-859.993] (-858.296) (-858.776) -- 0:01:37 513000 -- [-862.226] (-861.328) (-866.873) (-855.216) * (-874.921) (-865.796) (-870.451) [-854.842] -- 0:01:36 514000 -- [-860.426] (-865.108) (-859.872) (-863.202) * (-866.662) [-855.554] (-871.954) (-862.275) -- 0:01:37 515000 -- [-855.626] (-861.402) (-859.000) (-862.950) * (-862.808) (-857.772) (-867.404) [-863.467] -- 0:01:37 Average standard deviation of split frequencies: 0.007801 516000 -- (-860.884) (-866.866) [-858.654] (-861.313) * [-860.202] (-861.212) (-859.381) (-870.327) -- 0:01:36 517000 -- (-858.636) (-864.645) [-862.363] (-871.053) * [-861.545] (-856.595) (-858.937) (-862.647) -- 0:01:36 518000 -- (-860.053) [-860.164] (-858.633) (-861.964) * (-861.116) (-867.831) [-868.205] (-865.312) -- 0:01:36 519000 -- [-855.357] (-859.632) (-863.687) (-867.776) * (-856.086) [-857.985] (-852.083) (-853.652) -- 0:01:36 520000 -- (-861.753) (-853.225) (-858.214) [-859.851] * (-854.264) (-858.259) [-859.821] (-861.812) -- 0:01:36 Average standard deviation of split frequencies: 0.006686 521000 -- [-861.356] (-860.564) (-864.376) (-864.745) * (-855.897) (-861.121) [-857.740] (-865.317) -- 0:01:35 522000 -- (-868.396) (-859.326) (-858.633) [-856.856] * (-860.858) [-861.268] (-858.029) (-863.454) -- 0:01:35 523000 -- (-864.178) (-859.501) (-861.814) [-855.983] * (-854.511) (-861.741) [-857.075] (-854.128) -- 0:01:35 524000 -- (-871.504) (-864.196) (-869.902) [-856.310] * (-862.202) (-859.110) (-857.269) [-857.827] -- 0:01:35 525000 -- (-862.566) (-856.361) [-856.189] (-858.497) * (-871.057) (-859.612) (-856.352) [-857.192] -- 0:01:35 Average standard deviation of split frequencies: 0.006480 526000 -- [-856.383] (-860.104) (-862.753) (-858.950) * [-861.542] (-858.181) (-859.171) (-855.478) -- 0:01:34 527000 -- [-863.156] (-864.821) (-853.801) (-871.203) * (-860.670) [-860.381] (-873.131) (-852.422) -- 0:01:34 528000 -- (-861.096) (-870.483) (-861.188) [-857.374] * [-854.576] (-859.932) (-859.584) (-861.756) -- 0:01:34 529000 -- (-861.116) (-867.536) [-861.439] (-860.792) * [-856.748] (-861.238) (-855.104) (-861.169) -- 0:01:34 530000 -- (-863.805) (-862.200) [-857.665] (-860.009) * (-861.666) [-859.541] (-856.132) (-860.464) -- 0:01:34 Average standard deviation of split frequencies: 0.006013 531000 -- [-860.723] (-854.567) (-854.137) (-862.868) * (-856.803) (-865.357) (-854.245) [-866.542] -- 0:01:33 532000 -- (-860.591) (-861.555) (-864.312) [-857.830] * [-855.915] (-862.810) (-854.196) (-862.331) -- 0:01:33 533000 -- [-857.528] (-878.223) (-859.677) (-859.722) * (-858.246) (-856.310) [-854.049] (-860.211) -- 0:01:33 534000 -- (-857.482) [-865.653] (-855.842) (-858.515) * [-861.613] (-864.070) (-854.999) (-857.868) -- 0:01:33 535000 -- (-864.501) (-874.224) (-859.167) [-857.926] * (-867.038) (-858.275) [-859.163] (-856.148) -- 0:01:33 Average standard deviation of split frequencies: 0.006224 536000 -- [-858.197] (-875.599) (-867.440) (-857.047) * (-861.202) (-858.160) (-861.113) [-862.004] -- 0:01:32 537000 -- [-854.616] (-870.471) (-863.015) (-862.880) * [-859.434] (-859.157) (-856.487) (-858.844) -- 0:01:32 538000 -- [-855.853] (-863.762) (-864.522) (-852.683) * (-862.034) (-854.105) (-864.609) [-856.099] -- 0:01:32 539000 -- [-856.337] (-862.396) (-860.487) (-850.673) * (-864.832) (-853.007) (-868.979) [-858.962] -- 0:01:32 540000 -- [-856.532] (-860.077) (-860.464) (-858.867) * (-859.705) (-868.389) (-870.506) [-855.905] -- 0:01:32 Average standard deviation of split frequencies: 0.005902 541000 -- (-861.550) (-858.198) [-855.356] (-855.365) * [-856.364] (-862.919) (-863.706) (-864.348) -- 0:01:31 542000 -- (-865.282) (-863.358) (-857.194) [-857.433] * [-858.663] (-862.172) (-861.267) (-863.990) -- 0:01:31 543000 -- (-862.317) [-858.443] (-854.284) (-862.573) * [-860.345] (-857.250) (-857.427) (-856.752) -- 0:01:31 544000 -- (-862.834) (-858.456) [-863.018] (-857.682) * (-858.291) (-859.321) [-851.469] (-857.807) -- 0:01:31 545000 -- [-859.277] (-860.756) (-863.115) (-856.397) * (-858.764) (-855.989) (-861.039) [-856.794] -- 0:01:31 Average standard deviation of split frequencies: 0.005712 546000 -- (-858.023) (-864.141) (-863.064) [-860.247] * (-853.296) (-856.744) [-857.190] (-854.187) -- 0:01:30 547000 -- (-864.968) [-852.135] (-868.948) (-863.580) * (-866.660) (-860.190) [-856.890] (-859.526) -- 0:01:30 548000 -- (-856.451) (-862.015) (-864.868) [-860.540] * (-866.134) (-859.242) (-865.209) [-862.611] -- 0:01:30 549000 -- [-857.068] (-856.722) (-866.460) (-862.168) * (-860.929) (-867.486) [-854.454] (-857.578) -- 0:01:30 550000 -- (-867.137) [-862.243] (-868.935) (-855.337) * [-856.811] (-865.971) (-856.286) (-862.984) -- 0:01:30 Average standard deviation of split frequencies: 0.005202 551000 -- (-860.611) (-853.910) (-861.954) [-853.885] * (-859.681) [-860.037] (-856.414) (-857.321) -- 0:01:29 552000 -- [-855.034] (-859.894) (-857.705) (-854.654) * [-857.108] (-859.474) (-862.071) (-865.695) -- 0:01:29 553000 -- (-867.211) [-856.217] (-858.553) (-862.851) * [-860.288] (-854.365) (-869.371) (-856.512) -- 0:01:29 554000 -- (-865.210) (-855.806) [-853.567] (-858.556) * [-861.250] (-855.626) (-863.532) (-857.947) -- 0:01:29 555000 -- (-858.995) [-860.057] (-859.633) (-856.561) * (-858.470) (-855.674) (-855.858) [-857.244] -- 0:01:29 Average standard deviation of split frequencies: 0.004891 556000 -- (-860.986) (-861.287) [-859.154] (-865.749) * (-856.354) [-858.822] (-858.322) (-855.924) -- 0:01:28 557000 -- (-860.910) (-857.041) [-861.516] (-864.398) * [-856.736] (-863.723) (-861.975) (-856.934) -- 0:01:28 558000 -- (-854.746) [-850.856] (-859.466) (-858.301) * (-867.542) [-860.803] (-868.537) (-854.658) -- 0:01:28 559000 -- (-863.696) (-861.953) [-856.105] (-863.832) * (-868.741) (-862.707) [-857.204] (-855.706) -- 0:01:28 560000 -- (-869.883) [-854.030] (-864.414) (-862.403) * (-859.689) [-860.008] (-862.453) (-862.476) -- 0:01:28 Average standard deviation of split frequencies: 0.004721 561000 -- [-865.928] (-858.260) (-857.192) (-859.474) * (-868.737) (-858.387) [-864.473] (-857.596) -- 0:01:27 562000 -- (-863.305) (-855.257) (-857.352) [-857.869] * [-858.748] (-860.241) (-863.307) (-854.397) -- 0:01:27 563000 -- (-865.128) (-867.446) (-866.784) [-855.738] * [-857.057] (-860.615) (-864.468) (-858.686) -- 0:01:27 564000 -- (-860.838) (-865.959) (-867.749) [-854.590] * (-860.197) (-864.990) (-867.115) [-857.525] -- 0:01:27 565000 -- (-862.226) [-864.042] (-860.084) (-858.545) * (-867.450) [-859.344] (-871.083) (-870.184) -- 0:01:27 Average standard deviation of split frequencies: 0.005446 566000 -- (-861.000) (-863.219) [-855.663] (-863.529) * (-862.550) (-856.774) [-863.504] (-867.454) -- 0:01:26 567000 -- (-859.240) [-858.577] (-855.766) (-857.036) * (-856.761) (-862.815) (-864.374) [-853.390] -- 0:01:26 568000 -- [-866.219] (-855.497) (-857.974) (-864.189) * (-861.102) [-858.062] (-866.060) (-858.998) -- 0:01:26 569000 -- (-857.461) (-865.071) [-856.990] (-870.046) * (-864.362) (-862.758) [-860.615] (-857.075) -- 0:01:26 570000 -- (-858.880) [-852.972] (-856.721) (-866.663) * [-864.794] (-860.498) (-857.106) (-860.520) -- 0:01:26 Average standard deviation of split frequencies: 0.004384 571000 -- [-854.062] (-857.349) (-862.175) (-868.464) * (-859.156) [-860.134] (-857.538) (-867.024) -- 0:01:25 572000 -- (-860.959) (-869.373) [-858.040] (-857.990) * (-863.709) (-861.518) [-859.955] (-864.933) -- 0:01:25 573000 -- (-861.650) (-860.659) (-863.972) [-860.540] * (-860.261) (-867.595) [-859.117] (-860.656) -- 0:01:25 574000 -- (-858.439) (-869.159) (-863.784) [-856.826] * (-854.919) [-858.017] (-865.290) (-865.766) -- 0:01:25 575000 -- (-861.394) (-858.830) (-858.828) [-855.316] * (-860.683) (-861.095) (-856.602) [-860.522] -- 0:01:25 Average standard deviation of split frequencies: 0.004092 576000 -- (-858.353) [-858.719] (-850.331) (-855.655) * (-867.287) (-862.494) [-853.120] (-864.652) -- 0:01:24 577000 -- (-856.476) [-856.514] (-863.169) (-854.320) * [-861.523] (-856.453) (-854.629) (-864.871) -- 0:01:24 578000 -- (-865.279) (-860.606) (-863.621) [-862.806] * (-861.111) [-859.301] (-856.174) (-859.991) -- 0:01:24 579000 -- (-858.888) (-864.613) (-866.214) [-857.378] * (-858.847) [-857.426] (-867.877) (-868.839) -- 0:01:24 580000 -- (-864.263) [-856.185] (-866.586) (-860.428) * (-860.732) (-864.588) (-864.097) [-855.286] -- 0:01:24 Average standard deviation of split frequencies: 0.003560 581000 -- [-861.732] (-856.742) (-861.024) (-856.166) * (-860.021) [-857.894] (-858.921) (-857.778) -- 0:01:23 582000 -- (-858.566) [-863.681] (-860.008) (-865.588) * (-865.254) [-860.158] (-858.184) (-857.672) -- 0:01:23 583000 -- (-856.529) (-864.697) [-860.026] (-858.065) * (-860.130) (-861.352) (-863.420) [-857.385] -- 0:01:23 584000 -- (-855.782) (-854.181) [-861.322] (-861.665) * (-861.657) (-861.023) [-856.896] (-859.979) -- 0:01:23 585000 -- (-873.751) (-859.913) [-856.694] (-861.724) * (-853.619) [-853.923] (-857.982) (-859.391) -- 0:01:23 Average standard deviation of split frequencies: 0.004208 586000 -- [-864.653] (-862.193) (-855.035) (-869.837) * (-867.674) (-866.078) (-859.399) [-858.640] -- 0:01:22 587000 -- (-868.747) (-865.330) [-857.574] (-860.920) * (-860.638) (-859.517) [-856.388] (-856.220) -- 0:01:22 588000 -- [-860.950] (-868.237) (-857.067) (-856.878) * (-863.169) (-858.228) (-867.033) [-860.846] -- 0:01:22 589000 -- (-868.176) [-854.465] (-857.575) (-863.014) * (-860.592) (-855.187) [-862.701] (-861.425) -- 0:01:22 590000 -- (-859.010) [-858.508] (-859.302) (-860.799) * (-859.732) (-868.041) (-856.317) [-855.308] -- 0:01:22 Average standard deviation of split frequencies: 0.003929 591000 -- (-861.784) (-857.828) [-855.289] (-865.378) * [-860.719] (-861.406) (-855.453) (-858.067) -- 0:01:21 592000 -- [-861.879] (-859.922) (-855.112) (-867.679) * (-861.598) [-857.544] (-860.822) (-862.170) -- 0:01:21 593000 -- (-868.475) (-863.097) [-854.026] (-862.885) * (-861.325) [-863.198] (-855.401) (-863.434) -- 0:01:21 594000 -- (-867.837) (-856.213) [-858.700] (-860.362) * (-865.842) (-861.310) (-859.204) [-856.883] -- 0:01:21 595000 -- (-852.803) [-862.484] (-858.332) (-858.351) * [-854.948] (-861.454) (-860.222) (-854.084) -- 0:01:21 Average standard deviation of split frequencies: 0.004867 596000 -- (-861.189) (-857.536) (-854.336) [-857.945] * [-857.663] (-862.554) (-860.469) (-858.266) -- 0:01:20 597000 -- (-860.264) (-864.146) [-858.544] (-857.848) * (-859.604) (-858.774) (-852.953) [-856.996] -- 0:01:20 598000 -- (-860.623) (-865.195) (-858.699) [-854.565] * (-862.887) (-857.375) [-862.150] (-859.248) -- 0:01:20 599000 -- (-864.175) (-852.638) (-857.027) [-857.789] * [-858.074] (-860.915) (-857.205) (-858.312) -- 0:01:20 600000 -- (-860.272) (-857.447) [-854.166] (-855.668) * (-857.119) (-864.200) (-865.924) [-858.571] -- 0:01:20 Average standard deviation of split frequencies: 0.005192 601000 -- [-854.971] (-865.007) (-861.000) (-859.628) * (-861.260) (-863.052) [-855.287] (-859.781) -- 0:01:19 602000 -- [-853.773] (-863.501) (-861.074) (-859.214) * (-863.167) (-860.028) (-862.047) [-856.361] -- 0:01:19 603000 -- [-861.301] (-861.021) (-854.684) (-863.705) * (-853.372) (-857.985) [-860.871] (-862.952) -- 0:01:19 604000 -- [-859.675] (-857.591) (-864.897) (-862.700) * [-855.363] (-856.997) (-856.974) (-861.411) -- 0:01:19 605000 -- (-863.419) [-855.433] (-856.630) (-860.585) * (-855.858) [-855.882] (-871.358) (-861.628) -- 0:01:19 Average standard deviation of split frequencies: 0.004787 606000 -- (-858.540) (-856.225) [-857.259] (-858.326) * [-854.399] (-861.752) (-864.504) (-865.209) -- 0:01:18 607000 -- (-855.379) (-858.102) [-858.406] (-862.113) * [-858.860] (-864.420) (-857.318) (-858.916) -- 0:01:18 608000 -- (-866.570) [-857.358] (-854.715) (-857.315) * [-856.601] (-856.643) (-862.872) (-865.182) -- 0:01:18 609000 -- [-858.828] (-857.405) (-862.777) (-857.495) * (-861.875) (-857.089) [-860.018] (-864.341) -- 0:01:18 610000 -- [-854.524] (-858.307) (-860.408) (-855.928) * (-861.309) [-862.297] (-858.593) (-858.668) -- 0:01:18 Average standard deviation of split frequencies: 0.004394 611000 -- (-860.765) (-867.216) [-861.475] (-861.281) * [-857.419] (-858.715) (-860.731) (-858.288) -- 0:01:17 612000 -- (-857.620) (-863.572) (-863.275) [-859.654] * (-854.932) (-864.388) [-859.886] (-863.112) -- 0:01:17 613000 -- [-859.374] (-858.623) (-864.108) (-856.585) * [-856.728] (-857.810) (-858.877) (-856.878) -- 0:01:17 614000 -- (-861.802) (-852.748) [-858.442] (-862.054) * (-861.712) (-854.948) (-856.151) [-853.201] -- 0:01:17 615000 -- (-863.997) [-854.784] (-862.841) (-854.838) * (-860.578) [-853.742] (-861.916) (-858.330) -- 0:01:17 Average standard deviation of split frequencies: 0.004180 616000 -- (-863.854) (-863.412) (-862.665) [-858.851] * (-863.638) [-858.529] (-855.938) (-859.808) -- 0:01:16 617000 -- [-858.491] (-862.831) (-871.171) (-854.985) * (-862.322) [-857.212] (-858.086) (-866.824) -- 0:01:16 618000 -- (-863.563) (-861.600) (-865.093) [-858.169] * (-856.816) [-859.383] (-857.993) (-862.260) -- 0:01:16 619000 -- (-861.495) (-861.879) (-854.086) [-856.603] * [-858.095] (-858.711) (-858.338) (-865.451) -- 0:01:16 620000 -- (-865.183) [-858.054] (-864.029) (-860.136) * (-859.929) [-857.496] (-869.027) (-863.815) -- 0:01:16 Average standard deviation of split frequencies: 0.004849 621000 -- (-861.950) (-857.982) [-868.877] (-862.080) * (-858.946) (-855.411) [-856.016] (-861.639) -- 0:01:15 622000 -- (-863.823) (-865.405) [-853.619] (-857.057) * (-865.173) (-861.348) [-856.047] (-858.190) -- 0:01:15 623000 -- (-857.983) (-853.615) [-859.411] (-861.873) * (-866.694) [-853.419] (-857.407) (-858.269) -- 0:01:15 624000 -- (-860.971) (-855.420) [-858.417] (-866.415) * [-856.617] (-863.696) (-859.786) (-853.444) -- 0:01:15 625000 -- (-857.948) [-857.876] (-858.753) (-867.517) * (-861.309) (-861.513) [-854.006] (-863.416) -- 0:01:15 Average standard deviation of split frequencies: 0.005271 626000 -- (-861.766) [-855.565] (-865.585) (-870.652) * (-868.136) (-858.580) [-860.266] (-860.329) -- 0:01:14 627000 -- (-855.996) (-859.580) (-855.141) [-860.692] * [-860.644] (-862.401) (-864.665) (-859.255) -- 0:01:14 628000 -- (-857.579) (-862.343) (-854.195) [-856.154] * (-869.876) (-862.220) (-866.222) [-855.687] -- 0:01:14 629000 -- (-863.114) (-867.227) [-858.215] (-864.350) * [-863.419] (-860.558) (-861.096) (-864.767) -- 0:01:14 630000 -- (-860.813) (-858.428) [-859.822] (-872.115) * (-863.225) [-857.398] (-867.331) (-860.170) -- 0:01:14 Average standard deviation of split frequencies: 0.005865 631000 -- [-860.197] (-867.390) (-860.995) (-862.545) * (-858.222) [-855.707] (-867.147) (-864.077) -- 0:01:13 632000 -- (-858.973) [-859.392] (-865.020) (-864.402) * (-859.566) (-855.179) (-872.588) [-860.989] -- 0:01:13 633000 -- (-864.383) (-859.254) [-853.774] (-864.592) * [-859.270] (-859.134) (-857.535) (-865.064) -- 0:01:13 634000 -- (-859.631) (-860.379) [-855.955] (-860.083) * [-861.311] (-855.316) (-856.093) (-863.188) -- 0:01:13 635000 -- [-858.200] (-865.509) (-859.177) (-866.818) * [-857.248] (-855.786) (-864.998) (-859.997) -- 0:01:13 Average standard deviation of split frequencies: 0.005759 636000 -- (-855.271) (-856.380) [-855.087] (-860.608) * [-864.543] (-861.359) (-862.906) (-860.959) -- 0:01:12 637000 -- (-862.778) (-861.849) [-855.377] (-855.489) * [-861.540] (-866.847) (-859.053) (-857.448) -- 0:01:12 638000 -- [-856.158] (-866.218) (-862.744) (-859.431) * (-859.442) (-854.860) [-854.032] (-860.462) -- 0:01:12 639000 -- (-860.002) (-861.691) [-865.733] (-859.775) * (-858.643) [-860.868] (-856.410) (-856.401) -- 0:01:12 640000 -- (-868.415) [-855.789] (-862.445) (-858.748) * [-862.160] (-857.719) (-856.834) (-865.753) -- 0:01:12 Average standard deviation of split frequencies: 0.005830 641000 -- (-857.533) (-862.084) [-854.630] (-867.713) * (-857.320) (-857.519) [-854.842] (-858.757) -- 0:01:11 642000 -- (-855.243) (-859.886) [-856.950] (-859.735) * (-858.942) (-859.296) (-861.285) [-862.233] -- 0:01:11 643000 -- (-870.953) (-858.754) [-853.915] (-871.411) * [-855.546] (-866.535) (-858.098) (-860.640) -- 0:01:11 644000 -- (-863.769) (-861.548) (-855.981) [-859.683] * (-858.822) (-859.022) (-874.902) [-867.625] -- 0:01:11 645000 -- [-859.580] (-865.829) (-865.416) (-859.853) * (-866.026) [-854.355] (-868.639) (-868.746) -- 0:01:11 Average standard deviation of split frequencies: 0.005501 646000 -- (-855.609) (-861.577) [-862.596] (-857.987) * [-856.107] (-855.936) (-864.850) (-861.392) -- 0:01:10 647000 -- (-859.555) (-860.003) [-858.106] (-860.294) * [-855.198] (-861.369) (-862.976) (-859.554) -- 0:01:10 648000 -- (-857.809) (-869.529) (-856.591) [-857.343] * (-859.256) (-868.592) [-858.158] (-860.324) -- 0:01:10 649000 -- (-858.791) (-865.161) (-861.966) [-857.676] * (-857.978) (-864.359) [-856.043] (-864.540) -- 0:01:10 650000 -- (-858.280) (-863.650) (-865.224) [-859.154] * [-858.264] (-857.634) (-856.391) (-860.886) -- 0:01:10 Average standard deviation of split frequencies: 0.005573 651000 -- (-861.101) (-872.480) (-859.522) [-858.092] * (-865.374) (-854.429) (-860.389) [-859.776] -- 0:01:09 652000 -- (-859.417) (-858.992) (-856.591) [-859.541] * [-864.236] (-856.516) (-858.150) (-857.722) -- 0:01:09 653000 -- (-855.682) [-853.776] (-860.999) (-868.448) * (-863.701) [-855.359] (-866.730) (-857.287) -- 0:01:09 654000 -- [-858.358] (-864.634) (-860.496) (-854.507) * [-859.999] (-866.664) (-860.069) (-855.637) -- 0:01:09 655000 -- (-858.639) (-859.585) (-859.027) [-858.417] * (-871.264) (-856.070) (-857.681) [-856.790] -- 0:01:09 Average standard deviation of split frequencies: 0.005749 656000 -- (-860.318) [-855.381] (-862.274) (-860.245) * (-864.075) [-861.065] (-855.532) (-856.915) -- 0:01:08 657000 -- (-856.488) (-873.428) (-858.325) [-855.719] * (-860.797) [-855.526] (-860.976) (-868.619) -- 0:01:08 658000 -- (-856.961) (-856.208) (-861.136) [-859.160] * [-854.001] (-861.107) (-866.170) (-863.125) -- 0:01:08 659000 -- (-863.726) [-855.332] (-858.561) (-860.167) * (-859.154) (-862.723) (-865.634) [-855.170] -- 0:01:08 660000 -- (-859.707) [-859.099] (-859.984) (-857.951) * (-861.290) (-864.937) [-857.280] (-860.157) -- 0:01:08 Average standard deviation of split frequencies: 0.004940 661000 -- [-856.292] (-861.576) (-875.288) (-856.754) * [-855.854] (-860.423) (-857.578) (-862.310) -- 0:01:07 662000 -- (-863.497) (-864.565) [-859.872] (-861.636) * (-858.017) (-866.087) (-858.245) [-871.883] -- 0:01:07 663000 -- (-857.398) (-863.356) [-861.049] (-853.383) * [-856.885] (-868.533) (-860.827) (-860.800) -- 0:01:07 664000 -- [-864.129] (-856.202) (-864.855) (-856.534) * (-863.461) (-862.767) [-855.758] (-864.548) -- 0:01:07 665000 -- (-861.588) [-858.517] (-862.123) (-858.206) * (-862.639) (-860.387) [-859.192] (-860.995) -- 0:01:07 Average standard deviation of split frequencies: 0.004682 666000 -- (-857.387) (-859.701) [-858.578] (-855.781) * (-860.849) [-853.618] (-863.220) (-855.186) -- 0:01:06 667000 -- (-859.580) [-860.206] (-856.604) (-855.221) * [-860.381] (-862.219) (-870.447) (-863.372) -- 0:01:06 668000 -- (-858.621) (-855.778) (-860.390) [-864.279] * (-855.165) (-858.754) [-862.321] (-866.842) -- 0:01:06 669000 -- (-868.733) (-856.076) [-859.799] (-859.879) * [-861.618] (-854.025) (-860.418) (-859.524) -- 0:01:06 670000 -- (-868.727) (-864.240) (-862.272) [-864.867] * [-857.750] (-864.697) (-865.176) (-860.615) -- 0:01:06 Average standard deviation of split frequencies: 0.004596 671000 -- [-859.534] (-863.839) (-866.906) (-861.200) * (-854.720) (-859.141) [-859.290] (-857.358) -- 0:01:05 672000 -- (-857.068) (-859.142) (-863.636) [-861.173] * (-861.487) (-857.207) [-854.047] (-856.223) -- 0:01:05 673000 -- (-856.579) [-855.549] (-861.679) (-858.413) * (-862.399) (-859.487) (-858.812) [-858.114] -- 0:01:05 674000 -- (-854.432) (-859.204) [-855.761] (-859.849) * (-861.149) (-859.408) (-856.789) [-855.321] -- 0:01:05 675000 -- [-854.727] (-858.705) (-858.341) (-869.299) * (-862.161) (-857.858) (-861.593) [-855.973] -- 0:01:05 Average standard deviation of split frequencies: 0.005096 676000 -- (-857.358) [-865.450] (-858.988) (-857.258) * (-858.472) (-860.985) (-858.790) [-853.347] -- 0:01:04 677000 -- (-858.609) (-861.390) [-863.110] (-865.795) * [-857.988] (-860.670) (-861.905) (-864.876) -- 0:01:04 678000 -- (-865.108) [-863.993] (-855.493) (-862.028) * [-854.273] (-860.652) (-865.646) (-857.528) -- 0:01:04 679000 -- [-857.066] (-859.413) (-857.871) (-860.382) * (-853.094) (-862.472) [-856.971] (-865.119) -- 0:01:04 680000 -- (-856.385) [-855.463] (-856.119) (-862.400) * (-860.833) (-858.103) (-857.735) [-861.844] -- 0:01:04 Average standard deviation of split frequencies: 0.005061 681000 -- [-857.258] (-862.845) (-860.313) (-857.424) * [-855.651] (-858.572) (-864.883) (-855.691) -- 0:01:03 682000 -- (-860.746) (-867.866) [-854.212] (-860.237) * (-865.459) (-859.367) [-860.588] (-857.182) -- 0:01:03 683000 -- (-854.962) [-862.862] (-860.611) (-859.301) * (-858.700) [-860.423] (-858.012) (-856.251) -- 0:01:03 684000 -- (-863.358) (-865.200) [-858.102] (-861.428) * (-860.208) (-858.622) (-856.653) [-853.310] -- 0:01:03 685000 -- (-862.709) (-870.816) [-862.704] (-857.293) * (-854.897) (-855.934) [-861.185] (-859.160) -- 0:01:03 Average standard deviation of split frequencies: 0.004229 686000 -- [-852.116] (-854.057) (-864.321) (-856.002) * (-864.061) [-858.601] (-854.587) (-861.428) -- 0:01:02 687000 -- (-859.377) (-867.661) (-854.040) [-857.892] * (-859.557) (-864.202) (-866.414) [-853.930] -- 0:01:02 688000 -- (-865.488) (-862.174) [-859.353] (-867.901) * (-856.805) (-860.455) (-863.288) [-857.160] -- 0:01:02 689000 -- (-868.688) (-868.581) [-862.402] (-870.954) * (-857.678) [-856.318] (-870.656) (-860.583) -- 0:01:02 690000 -- (-858.825) [-852.753] (-867.359) (-863.179) * (-863.202) (-859.685) (-861.028) [-857.028] -- 0:01:02 Average standard deviation of split frequencies: 0.004725 691000 -- (-860.510) (-864.680) [-863.270] (-858.651) * [-857.683] (-861.045) (-860.498) (-859.556) -- 0:01:01 692000 -- (-857.615) (-857.188) (-863.159) [-857.872] * (-856.635) (-861.473) [-859.403] (-861.249) -- 0:01:01 693000 -- (-858.984) [-862.270] (-865.081) (-856.714) * [-860.199] (-860.308) (-860.212) (-858.826) -- 0:01:01 694000 -- [-858.840] (-865.693) (-865.409) (-853.948) * (-854.338) (-855.801) (-856.641) [-856.925] -- 0:01:01 695000 -- (-859.570) [-858.873] (-871.485) (-857.329) * (-858.752) (-857.377) (-871.684) [-857.361] -- 0:01:01 Average standard deviation of split frequencies: 0.005366 696000 -- [-856.080] (-870.177) (-862.546) (-856.018) * (-857.805) [-858.263] (-857.861) (-861.766) -- 0:01:00 697000 -- (-862.680) (-861.740) [-856.551] (-865.117) * (-858.380) (-856.785) (-873.442) [-856.487] -- 0:01:00 698000 -- (-856.315) [-857.621] (-858.387) (-857.993) * (-853.380) (-863.552) (-862.443) [-861.245] -- 0:01:00 699000 -- (-864.858) [-855.367] (-859.209) (-865.035) * [-855.099] (-854.882) (-866.718) (-854.638) -- 0:01:00 700000 -- [-859.497] (-856.922) (-866.820) (-862.155) * (-857.694) (-859.308) (-866.487) [-853.102] -- 0:01:00 Average standard deviation of split frequencies: 0.005124 701000 -- (-870.009) (-857.519) [-859.641] (-860.793) * [-862.302] (-857.324) (-859.215) (-867.018) -- 0:00:59 702000 -- [-861.972] (-856.764) (-858.696) (-863.414) * (-864.530) [-858.761] (-860.795) (-859.710) -- 0:00:59 703000 -- (-867.507) (-858.002) [-865.870] (-858.872) * (-859.295) (-864.112) [-859.898] (-859.626) -- 0:00:59 704000 -- [-861.772] (-858.646) (-865.001) (-854.500) * [-861.349] (-862.521) (-868.550) (-860.016) -- 0:00:59 705000 -- (-854.749) [-859.785] (-857.080) (-860.349) * (-868.305) (-858.940) [-856.164] (-863.956) -- 0:00:59 Average standard deviation of split frequencies: 0.005085 706000 -- (-858.569) (-860.113) [-859.507] (-861.428) * (-858.107) (-854.978) (-859.721) [-863.417] -- 0:00:58 707000 -- (-856.381) (-859.895) (-869.589) [-853.684] * (-857.451) (-862.605) (-861.492) [-864.100] -- 0:00:58 708000 -- (-862.215) (-866.281) (-857.727) [-862.143] * [-857.489] (-858.953) (-857.630) (-858.760) -- 0:00:58 709000 -- (-857.960) (-860.114) (-861.135) [-856.735] * (-862.097) (-864.629) [-865.693] (-871.257) -- 0:00:58 710000 -- [-858.739] (-865.290) (-864.812) (-858.636) * (-872.289) (-859.035) [-860.436] (-868.223) -- 0:00:58 Average standard deviation of split frequencies: 0.005051 711000 -- (-862.040) [-864.469] (-863.357) (-861.083) * (-858.554) [-860.907] (-857.952) (-865.287) -- 0:00:57 712000 -- [-857.227] (-864.632) (-860.635) (-859.648) * (-857.282) (-865.067) [-864.960] (-857.817) -- 0:00:57 713000 -- (-861.213) (-861.158) (-859.742) [-858.983] * [-854.868] (-858.874) (-857.498) (-857.918) -- 0:00:57 714000 -- (-860.633) (-858.475) (-863.044) [-860.541] * (-863.268) (-865.841) [-860.617] (-863.874) -- 0:00:57 715000 -- (-858.362) (-863.723) [-857.475] (-861.944) * [-854.090] (-860.076) (-865.060) (-861.148) -- 0:00:57 Average standard deviation of split frequencies: 0.004913 716000 -- (-857.678) (-862.683) [-853.227] (-859.621) * (-858.448) (-866.232) [-860.989] (-866.408) -- 0:00:56 717000 -- (-856.248) (-860.412) [-856.957] (-860.564) * (-864.570) [-858.467] (-859.583) (-852.972) -- 0:00:56 718000 -- (-857.633) (-862.720) (-855.719) [-855.208] * (-863.210) [-859.458] (-860.889) (-867.465) -- 0:00:56 719000 -- [-859.266] (-861.540) (-857.964) (-856.380) * (-867.216) (-859.060) [-856.587] (-860.486) -- 0:00:56 720000 -- (-853.539) (-856.013) [-854.384] (-860.558) * (-858.093) (-857.604) (-856.867) [-857.646] -- 0:00:56 Average standard deviation of split frequencies: 0.005082 721000 -- (-864.482) [-861.785] (-855.559) (-857.212) * (-864.157) [-860.770] (-868.485) (-865.254) -- 0:00:55 722000 -- (-857.935) (-861.777) [-855.265] (-853.251) * (-862.549) (-864.150) (-866.726) [-858.277] -- 0:00:55 723000 -- (-864.973) [-859.157] (-855.958) (-853.015) * (-858.072) (-863.426) [-861.737] (-861.869) -- 0:00:55 724000 -- (-860.063) (-857.360) [-856.006] (-862.553) * (-855.631) (-871.744) (-866.295) [-857.906] -- 0:00:55 725000 -- [-860.809] (-861.518) (-862.555) (-861.607) * [-857.312] (-863.229) (-856.885) (-865.110) -- 0:00:55 Average standard deviation of split frequencies: 0.005045 726000 -- (-856.320) [-855.410] (-861.254) (-866.384) * [-860.480] (-858.572) (-857.067) (-863.722) -- 0:00:54 727000 -- (-861.250) [-861.665] (-855.401) (-865.447) * (-865.919) [-860.642] (-856.419) (-865.359) -- 0:00:54 728000 -- (-857.575) (-859.989) (-854.365) [-854.708] * (-869.861) [-856.370] (-856.333) (-863.471) -- 0:00:54 729000 -- [-857.833] (-861.604) (-861.250) (-858.156) * (-859.319) (-865.181) (-853.173) [-858.093] -- 0:00:54 730000 -- (-860.645) (-859.995) [-854.470] (-858.395) * (-866.865) (-857.634) (-859.369) [-866.338] -- 0:00:54 Average standard deviation of split frequencies: 0.004913 731000 -- [-856.283] (-861.682) (-859.826) (-856.608) * (-868.769) (-857.601) [-857.339] (-864.918) -- 0:00:53 732000 -- (-864.434) [-860.934] (-857.705) (-857.188) * (-857.908) [-859.876] (-861.497) (-864.053) -- 0:00:53 733000 -- (-867.388) [-857.516] (-865.655) (-855.384) * (-864.587) (-863.911) [-857.421] (-861.864) -- 0:00:53 734000 -- (-863.771) [-861.360] (-861.145) (-859.567) * (-860.515) [-861.077] (-857.228) (-860.690) -- 0:00:53 735000 -- (-865.911) [-854.974] (-863.886) (-855.193) * (-865.697) (-868.860) (-862.383) [-865.667] -- 0:00:53 Average standard deviation of split frequencies: 0.005025 736000 -- (-866.816) [-859.772] (-851.617) (-863.590) * (-865.659) [-855.153] (-851.681) (-855.958) -- 0:00:52 737000 -- (-869.744) (-857.831) [-864.103] (-856.748) * [-861.104] (-856.599) (-856.784) (-855.147) -- 0:00:52 738000 -- (-871.492) (-860.551) (-854.566) [-859.531] * (-860.004) (-860.627) [-864.572] (-855.065) -- 0:00:52 739000 -- (-865.334) (-859.176) (-868.963) [-858.608] * (-854.706) [-867.879] (-867.572) (-858.205) -- 0:00:52 740000 -- (-857.056) (-864.749) (-860.926) [-856.030] * (-867.976) [-866.358] (-859.461) (-859.846) -- 0:00:52 Average standard deviation of split frequencies: 0.005973 741000 -- (-865.756) (-863.209) (-855.728) [-865.351] * (-858.552) (-865.445) (-862.314) [-861.330] -- 0:00:51 742000 -- (-853.446) [-859.167] (-863.497) (-868.198) * (-858.820) [-855.347] (-861.194) (-867.905) -- 0:00:51 743000 -- (-859.859) (-858.403) (-861.115) [-857.170] * (-859.602) (-856.239) [-863.910] (-867.036) -- 0:00:51 744000 -- (-857.700) (-863.674) (-855.909) [-857.943] * (-863.441) (-863.630) [-855.838] (-862.641) -- 0:00:51 745000 -- (-856.735) (-857.701) [-860.951] (-856.732) * (-856.870) (-860.378) [-856.634] (-867.696) -- 0:00:51 Average standard deviation of split frequencies: 0.006611 746000 -- [-865.534] (-853.218) (-861.764) (-858.270) * (-857.668) [-866.356] (-857.838) (-866.480) -- 0:00:50 747000 -- (-855.985) [-854.290] (-857.437) (-859.492) * (-854.994) (-858.592) [-860.097] (-860.516) -- 0:00:50 748000 -- (-862.125) (-860.040) [-864.497] (-865.796) * (-858.379) [-860.878] (-857.206) (-856.595) -- 0:00:50 749000 -- (-855.683) (-860.852) (-857.109) [-861.128] * (-860.453) [-854.486] (-866.368) (-860.174) -- 0:00:50 750000 -- [-855.044] (-864.123) (-862.113) (-862.183) * (-858.371) [-856.675] (-856.473) (-856.805) -- 0:00:50 Average standard deviation of split frequencies: 0.006715 751000 -- (-869.746) (-861.432) [-862.033] (-857.128) * (-855.636) (-862.354) [-862.556] (-863.112) -- 0:00:49 752000 -- (-858.331) (-857.736) (-851.844) [-862.631] * [-854.489] (-865.951) (-861.464) (-867.419) -- 0:00:49 753000 -- (-863.788) [-855.416] (-860.533) (-859.594) * [-856.810] (-863.578) (-864.737) (-866.028) -- 0:00:49 754000 -- (-869.202) (-868.098) [-857.501] (-857.022) * (-858.797) (-862.935) (-866.760) [-863.132] -- 0:00:49 755000 -- (-866.221) (-859.034) [-855.904] (-863.875) * [-862.521] (-873.079) (-868.653) (-857.502) -- 0:00:49 Average standard deviation of split frequencies: 0.007051 756000 -- (-862.462) [-854.555] (-859.285) (-857.106) * (-865.411) (-868.546) (-873.195) [-854.003] -- 0:00:48 757000 -- [-857.896] (-865.310) (-857.148) (-866.757) * (-863.070) (-861.985) (-866.543) [-857.000] -- 0:00:48 758000 -- (-856.781) [-860.243] (-863.703) (-857.482) * (-871.542) (-865.154) [-862.652] (-855.848) -- 0:00:48 759000 -- (-860.788) [-861.960] (-857.031) (-858.954) * (-863.715) [-866.217] (-861.370) (-859.451) -- 0:00:48 760000 -- (-862.058) [-858.771] (-856.195) (-861.013) * [-868.243] (-854.048) (-867.248) (-856.862) -- 0:00:48 Average standard deviation of split frequencies: 0.007198 761000 -- [-857.546] (-855.444) (-858.150) (-860.876) * (-860.365) (-850.756) [-856.161] (-856.077) -- 0:00:47 762000 -- (-858.986) (-855.642) (-859.862) [-859.775] * (-865.352) (-853.540) [-865.565] (-858.317) -- 0:00:47 763000 -- (-864.684) (-856.943) (-856.989) [-851.751] * (-865.026) (-868.502) [-853.555] (-858.832) -- 0:00:47 764000 -- [-858.767] (-860.273) (-854.773) (-858.536) * (-867.247) (-859.746) [-856.188] (-871.044) -- 0:00:47 765000 -- (-863.164) (-859.948) [-860.446] (-856.113) * [-860.701] (-869.442) (-856.485) (-858.386) -- 0:00:47 Average standard deviation of split frequencies: 0.007432 766000 -- (-868.305) (-858.398) [-857.166] (-862.527) * (-859.076) (-857.967) (-858.978) [-858.868] -- 0:00:46 767000 -- (-859.386) [-855.843] (-858.285) (-861.029) * (-857.825) (-855.158) (-864.321) [-856.013] -- 0:00:46 768000 -- (-861.335) [-859.922] (-860.008) (-860.800) * (-867.320) (-856.658) [-857.660] (-855.680) -- 0:00:46 769000 -- (-870.902) (-854.906) (-858.768) [-857.064] * (-862.187) [-856.478] (-861.435) (-861.711) -- 0:00:46 770000 -- (-860.028) [-854.754] (-861.281) (-860.898) * (-861.207) (-857.379) [-865.297] (-861.317) -- 0:00:46 Average standard deviation of split frequencies: 0.007623 771000 -- (-866.847) (-861.469) [-853.752] (-855.816) * (-871.120) (-864.844) (-858.848) [-861.231] -- 0:00:45 772000 -- (-865.862) [-862.091] (-858.080) (-859.300) * (-870.211) [-855.309] (-857.017) (-861.948) -- 0:00:45 773000 -- (-869.696) [-855.623] (-868.828) (-873.662) * (-863.233) (-856.348) (-860.033) [-860.550] -- 0:00:45 774000 -- [-862.457] (-863.332) (-859.213) (-864.724) * [-869.199] (-863.137) (-852.611) (-865.898) -- 0:00:45 775000 -- (-861.024) [-856.295] (-862.000) (-869.856) * [-860.484] (-852.114) (-863.703) (-859.921) -- 0:00:45 Average standard deviation of split frequencies: 0.007617 776000 -- (-854.487) (-860.393) (-873.792) [-857.777] * (-864.964) (-858.502) (-856.952) [-857.873] -- 0:00:44 777000 -- (-856.781) (-857.686) [-872.477] (-860.222) * (-863.737) [-855.179] (-863.580) (-856.346) -- 0:00:44 778000 -- (-856.802) [-859.676] (-866.596) (-864.546) * (-855.340) (-862.760) (-875.960) [-855.052] -- 0:00:44 779000 -- (-871.765) (-863.334) [-862.948] (-863.279) * (-862.804) (-857.930) [-863.601] (-865.296) -- 0:00:44 780000 -- (-865.984) [-854.140] (-858.007) (-857.880) * (-859.865) [-855.538] (-867.669) (-863.185) -- 0:00:44 Average standard deviation of split frequencies: 0.007339 781000 -- (-860.988) (-862.779) [-859.225] (-857.368) * (-864.330) [-855.477] (-870.067) (-868.621) -- 0:00:43 782000 -- (-860.304) [-855.842] (-859.881) (-866.904) * (-867.979) (-857.323) (-863.518) [-866.864] -- 0:00:43 783000 -- (-855.089) [-862.577] (-864.361) (-868.291) * (-860.461) (-862.546) (-860.686) [-864.633] -- 0:00:43 784000 -- [-852.726] (-859.385) (-855.687) (-858.867) * (-862.933) [-860.223] (-861.573) (-860.715) -- 0:00:43 785000 -- (-855.413) (-866.228) (-856.501) [-854.009] * (-861.892) [-858.633] (-856.437) (-859.405) -- 0:00:43 Average standard deviation of split frequencies: 0.007105 786000 -- (-855.720) [-859.204] (-865.597) (-870.447) * (-857.058) (-860.950) (-862.199) [-856.671] -- 0:00:42 787000 -- (-864.277) (-859.019) [-858.526] (-861.178) * (-862.557) (-857.512) [-856.806] (-860.122) -- 0:00:42 788000 -- (-863.314) [-862.382] (-853.137) (-864.714) * [-867.705] (-863.625) (-857.020) (-863.286) -- 0:00:42 789000 -- (-859.770) [-853.505] (-857.416) (-861.517) * (-866.241) [-860.957] (-859.272) (-866.908) -- 0:00:42 790000 -- (-866.182) (-855.014) [-857.062] (-869.090) * (-871.058) (-864.329) (-864.258) [-859.934] -- 0:00:42 Average standard deviation of split frequencies: 0.007246 791000 -- (-862.084) (-857.340) (-863.267) [-859.187] * [-856.575] (-867.427) (-860.327) (-866.993) -- 0:00:41 792000 -- (-863.283) [-855.184] (-861.146) (-861.049) * [-855.610] (-859.009) (-857.702) (-857.829) -- 0:00:41 793000 -- (-864.203) (-859.700) [-858.665] (-866.712) * (-856.747) (-857.047) [-856.344] (-862.852) -- 0:00:41 794000 -- [-858.977] (-861.789) (-858.648) (-863.164) * [-855.989] (-863.482) (-860.497) (-864.578) -- 0:00:41 795000 -- (-861.289) (-861.814) (-860.271) [-857.414] * (-859.891) (-858.783) (-854.327) [-859.537] -- 0:00:41 Average standard deviation of split frequencies: 0.007015 796000 -- (-858.011) (-855.131) [-856.789] (-863.492) * (-867.178) (-863.276) [-860.028] (-864.921) -- 0:00:40 797000 -- (-858.247) (-866.858) (-863.525) [-860.680] * (-856.733) (-856.925) [-858.047] (-862.230) -- 0:00:40 798000 -- (-860.746) (-860.633) [-850.263] (-860.477) * [-857.587] (-855.161) (-870.465) (-860.970) -- 0:00:40 799000 -- (-854.370) (-862.481) (-857.218) [-863.718] * (-856.586) (-857.247) [-851.972] (-866.226) -- 0:00:40 800000 -- (-860.025) (-862.006) [-856.154] (-868.582) * [-856.637] (-857.159) (-862.626) (-863.517) -- 0:00:40 Average standard deviation of split frequencies: 0.006884 801000 -- (-856.662) [-858.117] (-853.913) (-861.788) * (-860.539) (-858.882) (-861.517) [-856.762] -- 0:00:39 802000 -- (-858.746) [-858.191] (-857.936) (-858.975) * (-857.841) (-856.810) (-858.619) [-860.511] -- 0:00:39 803000 -- (-854.937) (-853.262) [-854.517] (-858.514) * [-860.854] (-854.296) (-867.154) (-854.350) -- 0:00:39 804000 -- (-860.545) [-857.186] (-853.869) (-858.449) * (-860.254) (-855.214) (-862.961) [-857.195] -- 0:00:39 805000 -- (-861.775) (-850.857) [-858.074] (-858.136) * (-857.292) (-863.657) [-856.140] (-860.539) -- 0:00:39 Average standard deviation of split frequencies: 0.006928 806000 -- (-858.011) (-857.982) [-861.470] (-862.401) * (-864.473) [-858.052] (-864.134) (-863.629) -- 0:00:38 807000 -- [-862.079] (-861.684) (-858.168) (-864.810) * (-860.081) (-863.705) (-856.577) [-857.345] -- 0:00:38 808000 -- [-855.330] (-873.365) (-862.080) (-855.448) * (-860.859) [-859.288] (-857.486) (-868.532) -- 0:00:38 809000 -- (-857.879) (-861.025) (-859.108) [-857.436] * (-863.670) (-867.327) (-860.286) [-858.826] -- 0:00:38 810000 -- (-854.997) (-860.139) (-856.746) [-854.251] * (-860.762) (-861.708) [-859.756] (-867.325) -- 0:00:38 Average standard deviation of split frequencies: 0.007157 811000 -- (-855.671) (-860.026) [-858.086] (-861.577) * (-859.824) [-859.154] (-856.806) (-863.142) -- 0:00:37 812000 -- (-857.864) (-862.265) (-860.050) [-861.104] * (-858.846) (-859.926) [-853.749] (-865.107) -- 0:00:37 813000 -- (-857.217) [-855.430] (-859.728) (-859.413) * (-852.852) (-864.456) (-859.169) [-856.244] -- 0:00:37 814000 -- (-861.980) [-858.943] (-858.813) (-859.146) * (-860.961) [-864.820] (-857.745) (-862.632) -- 0:00:37 815000 -- (-861.535) [-851.935] (-869.722) (-858.141) * (-865.594) (-859.726) [-855.104] (-867.017) -- 0:00:37 Average standard deviation of split frequencies: 0.006844 816000 -- (-861.324) (-861.474) [-864.352] (-860.502) * (-859.024) (-858.035) (-857.788) [-862.041] -- 0:00:36 817000 -- [-855.617] (-860.592) (-859.508) (-859.177) * (-862.947) [-853.795] (-864.212) (-862.698) -- 0:00:36 818000 -- [-855.320] (-863.155) (-863.891) (-858.249) * (-859.424) (-862.256) [-859.897] (-860.134) -- 0:00:36 819000 -- [-861.491] (-865.100) (-866.030) (-858.935) * [-853.894] (-868.448) (-858.161) (-859.377) -- 0:00:36 820000 -- (-863.173) (-867.575) (-858.828) [-852.152] * (-856.351) (-859.875) [-859.995] (-856.199) -- 0:00:36 Average standard deviation of split frequencies: 0.006628 821000 -- [-865.610] (-861.403) (-861.187) (-863.789) * [-860.380] (-856.689) (-863.069) (-868.775) -- 0:00:35 822000 -- (-867.774) (-862.455) (-858.569) [-856.809] * (-864.314) [-862.552] (-858.065) (-861.562) -- 0:00:35 823000 -- (-862.075) (-858.410) [-863.637] (-857.947) * (-856.807) [-856.439] (-861.008) (-862.756) -- 0:00:35 824000 -- (-861.686) (-857.898) [-855.970] (-856.896) * [-857.698] (-856.845) (-864.606) (-859.622) -- 0:00:35 825000 -- (-863.002) [-862.464] (-861.707) (-864.991) * [-856.369] (-852.586) (-863.961) (-864.646) -- 0:00:35 Average standard deviation of split frequencies: 0.006234 826000 -- (-858.934) (-867.616) (-858.619) [-859.554] * (-857.040) (-856.680) (-859.245) [-858.355] -- 0:00:34 827000 -- (-857.803) (-860.000) (-869.741) [-856.345] * (-864.415) (-858.519) [-863.825] (-858.277) -- 0:00:34 828000 -- (-862.834) [-852.039] (-854.065) (-868.875) * (-855.765) (-862.628) (-865.865) [-857.065] -- 0:00:34 829000 -- (-855.012) (-870.783) [-861.663] (-863.668) * (-860.082) (-871.387) (-857.420) [-858.173] -- 0:00:34 830000 -- (-851.698) (-855.213) [-860.305] (-868.351) * (-855.088) (-866.708) (-857.694) [-862.546] -- 0:00:34 Average standard deviation of split frequencies: 0.006286 831000 -- (-861.329) (-856.273) (-861.967) [-860.725] * [-859.659] (-856.079) (-859.902) (-865.263) -- 0:00:33 832000 -- (-856.581) (-871.146) [-858.303] (-854.192) * (-862.552) (-868.727) (-856.807) [-861.906] -- 0:00:33 833000 -- [-855.404] (-861.379) (-859.059) (-857.326) * [-858.912] (-864.672) (-860.043) (-861.576) -- 0:00:33 834000 -- (-857.238) [-861.559] (-866.247) (-867.084) * [-858.075] (-856.344) (-863.319) (-863.777) -- 0:00:33 835000 -- (-862.431) (-864.209) [-860.803] (-869.128) * [-859.578] (-865.987) (-861.308) (-861.133) -- 0:00:33 Average standard deviation of split frequencies: 0.006463 836000 -- (-862.703) (-854.353) [-856.997] (-862.821) * [-856.740] (-860.881) (-860.153) (-855.993) -- 0:00:32 837000 -- [-857.777] (-860.656) (-859.082) (-861.498) * (-856.455) [-856.370] (-861.847) (-864.489) -- 0:00:32 838000 -- (-857.990) (-870.780) (-867.681) [-862.392] * (-863.383) (-864.903) [-853.866] (-856.848) -- 0:00:32 839000 -- (-854.112) (-865.933) [-855.792] (-855.226) * [-853.987] (-862.709) (-857.071) (-859.912) -- 0:00:32 840000 -- (-861.281) (-858.196) [-863.291] (-861.688) * [-859.598] (-869.780) (-867.151) (-864.875) -- 0:00:32 Average standard deviation of split frequencies: 0.006858 841000 -- [-858.905] (-864.910) (-869.107) (-858.235) * [-857.872] (-866.753) (-858.006) (-858.516) -- 0:00:31 842000 -- (-857.189) [-851.315] (-861.744) (-861.932) * (-862.727) [-858.005] (-864.834) (-861.130) -- 0:00:31 843000 -- (-864.332) (-871.518) (-857.111) [-860.985] * (-864.996) (-864.980) [-858.100] (-854.902) -- 0:00:31 844000 -- (-864.060) [-859.605] (-863.425) (-856.273) * [-856.288] (-865.035) (-857.737) (-861.734) -- 0:00:31 845000 -- [-858.211] (-858.547) (-861.417) (-859.921) * (-861.837) (-856.660) [-856.127] (-863.129) -- 0:00:31 Average standard deviation of split frequencies: 0.007244 846000 -- (-860.081) (-862.304) (-860.138) [-856.682] * (-854.583) [-856.571] (-860.730) (-856.780) -- 0:00:30 847000 -- (-863.199) (-858.656) (-860.511) [-852.864] * (-866.382) (-857.955) [-859.228] (-855.605) -- 0:00:30 848000 -- [-854.681] (-861.993) (-860.271) (-860.120) * (-858.138) (-866.269) (-857.749) [-856.407] -- 0:00:30 849000 -- (-859.452) [-858.780] (-867.657) (-865.765) * (-863.917) [-860.846] (-860.577) (-856.872) -- 0:00:30 850000 -- (-856.610) (-867.116) (-862.581) [-863.227] * (-856.904) (-863.884) (-856.226) [-855.977] -- 0:00:30 Average standard deviation of split frequencies: 0.007034 851000 -- (-861.152) (-866.093) (-856.906) [-856.675] * (-863.508) [-863.393] (-865.839) (-862.881) -- 0:00:29 852000 -- (-859.306) (-864.321) [-862.152] (-865.899) * (-857.418) [-857.388] (-860.084) (-856.578) -- 0:00:29 853000 -- [-854.880] (-864.684) (-867.003) (-858.034) * (-858.188) [-857.024] (-858.372) (-855.197) -- 0:00:29 854000 -- (-857.255) [-856.339] (-858.212) (-862.823) * (-861.618) (-863.024) (-862.240) [-854.190] -- 0:00:29 855000 -- (-856.621) (-861.311) (-856.553) [-859.502] * (-865.852) (-873.370) (-861.768) [-858.947] -- 0:00:29 Average standard deviation of split frequencies: 0.007074 856000 -- (-859.278) (-861.626) (-854.906) [-858.522] * (-864.469) (-861.640) (-865.090) [-856.779] -- 0:00:28 857000 -- (-864.930) [-856.175] (-864.463) (-860.414) * (-856.550) (-866.463) (-868.708) [-859.484] -- 0:00:28 858000 -- (-857.048) (-857.212) [-856.985] (-858.443) * (-856.906) (-855.512) (-863.879) [-859.749] -- 0:00:28 859000 -- (-872.309) [-872.000] (-861.397) (-860.551) * (-866.820) [-859.303] (-859.650) (-858.275) -- 0:00:28 860000 -- (-859.346) (-862.604) (-861.307) [-853.649] * [-857.337] (-854.692) (-862.881) (-861.868) -- 0:00:28 Average standard deviation of split frequencies: 0.006994 861000 -- (-857.203) (-860.336) (-854.188) [-855.697] * (-857.568) [-857.827] (-860.363) (-861.758) -- 0:00:27 862000 -- [-861.322] (-875.182) (-861.848) (-856.150) * (-866.237) [-854.112] (-860.890) (-859.421) -- 0:00:27 863000 -- [-856.800] (-858.242) (-863.052) (-857.399) * (-873.031) (-857.647) [-855.195] (-857.969) -- 0:00:27 864000 -- [-858.000] (-853.662) (-857.377) (-861.672) * (-859.677) (-861.171) (-862.754) [-860.477] -- 0:00:27 865000 -- (-861.537) (-866.259) (-867.938) [-858.640] * [-855.302] (-868.328) (-861.202) (-867.219) -- 0:00:27 Average standard deviation of split frequencies: 0.007370 866000 -- [-855.023] (-861.927) (-867.614) (-862.995) * (-858.834) (-859.554) (-865.787) [-855.137] -- 0:00:26 867000 -- (-860.183) [-856.853] (-858.879) (-858.250) * (-870.086) (-860.837) [-855.389] (-862.371) -- 0:00:26 868000 -- (-859.300) (-856.073) (-860.396) [-862.265] * (-870.745) (-856.995) (-859.235) [-857.594] -- 0:00:26 869000 -- (-864.240) (-850.387) (-865.421) [-858.179] * (-857.818) (-866.271) [-858.250] (-860.379) -- 0:00:26 870000 -- (-858.599) [-859.682] (-861.782) (-857.326) * [-862.663] (-856.892) (-856.138) (-863.194) -- 0:00:26 Average standard deviation of split frequencies: 0.007080 871000 -- [-858.141] (-862.783) (-865.912) (-862.615) * (-862.562) (-859.673) [-854.007] (-861.472) -- 0:00:25 872000 -- [-865.814] (-861.900) (-859.803) (-856.793) * (-862.022) (-864.731) [-859.122] (-857.893) -- 0:00:25 873000 -- [-860.328] (-866.927) (-858.359) (-858.553) * (-861.563) (-862.627) (-861.845) [-857.316] -- 0:00:25 874000 -- [-860.812] (-861.578) (-866.547) (-855.004) * (-863.397) (-856.724) [-856.058] (-869.115) -- 0:00:25 875000 -- [-857.643] (-870.560) (-860.019) (-855.931) * [-865.614] (-860.907) (-860.071) (-864.263) -- 0:00:25 Average standard deviation of split frequencies: 0.007285 876000 -- (-860.082) [-864.851] (-862.543) (-860.326) * [-857.039] (-858.751) (-862.415) (-867.558) -- 0:00:24 877000 -- (-854.386) [-853.505] (-861.666) (-859.254) * (-855.587) (-865.518) [-854.243] (-862.663) -- 0:00:24 878000 -- (-855.010) (-857.182) (-858.598) [-859.693] * [-858.696] (-860.875) (-866.424) (-852.688) -- 0:00:24 879000 -- [-858.149] (-863.057) (-857.304) (-857.624) * (-864.046) (-856.290) [-859.969] (-856.806) -- 0:00:24 880000 -- (-861.398) (-858.618) [-856.951] (-856.763) * (-862.138) [-855.117] (-856.423) (-857.035) -- 0:00:24 Average standard deviation of split frequencies: 0.007535 881000 -- (-864.501) (-861.539) [-853.822] (-859.808) * (-855.907) (-876.851) [-858.430] (-864.781) -- 0:00:23 882000 -- (-856.289) (-866.381) (-858.614) [-863.569] * (-859.353) (-856.938) (-861.269) [-855.361] -- 0:00:23 883000 -- (-857.743) [-859.883] (-858.239) (-857.303) * (-865.761) (-856.584) [-857.688] (-855.990) -- 0:00:23 884000 -- [-855.490] (-871.672) (-858.368) (-856.660) * [-856.835] (-854.888) (-862.232) (-857.145) -- 0:00:23 885000 -- (-859.071) (-859.159) [-861.654] (-856.062) * [-858.827] (-864.225) (-869.932) (-865.315) -- 0:00:23 Average standard deviation of split frequencies: 0.007490 886000 -- [-853.516] (-863.010) (-854.444) (-860.091) * [-854.026] (-862.323) (-876.772) (-859.548) -- 0:00:22 887000 -- (-855.736) [-855.353] (-857.293) (-858.695) * (-863.904) (-859.208) (-863.892) [-860.230] -- 0:00:22 888000 -- (-860.823) [-855.162] (-857.594) (-861.383) * [-864.395] (-858.678) (-857.940) (-855.754) -- 0:00:22 889000 -- (-859.730) (-860.715) (-855.399) [-859.293] * (-855.001) (-862.683) (-855.402) [-856.743] -- 0:00:22 890000 -- (-858.701) (-862.025) (-861.446) [-857.573] * (-857.379) [-859.786] (-860.351) (-857.739) -- 0:00:22 Average standard deviation of split frequencies: 0.007369 891000 -- [-855.075] (-852.737) (-863.115) (-862.628) * (-851.440) (-865.287) (-868.711) [-854.796] -- 0:00:21 892000 -- [-855.457] (-858.608) (-862.171) (-872.186) * (-858.786) (-863.885) (-861.290) [-854.418] -- 0:00:21 893000 -- (-859.423) (-863.284) (-857.185) [-857.897] * (-858.594) [-856.839] (-861.737) (-867.576) -- 0:00:21 894000 -- [-857.368] (-862.350) (-859.341) (-861.369) * (-854.793) [-855.536] (-865.200) (-862.632) -- 0:00:21 895000 -- (-855.944) [-864.271] (-864.601) (-859.628) * [-858.441] (-863.850) (-861.338) (-859.485) -- 0:00:21 Average standard deviation of split frequencies: 0.007325 896000 -- (-858.312) (-854.826) (-858.993) [-855.357] * (-862.110) [-864.143] (-859.555) (-862.479) -- 0:00:20 897000 -- (-864.582) (-852.935) [-857.159] (-861.107) * (-855.665) [-862.521] (-868.754) (-864.858) -- 0:00:20 898000 -- [-855.390] (-859.968) (-856.947) (-858.765) * [-862.543] (-858.551) (-858.989) (-863.425) -- 0:00:20 899000 -- [-859.996] (-857.524) (-855.805) (-862.951) * (-859.456) (-856.292) [-858.412] (-859.739) -- 0:00:20 900000 -- (-856.156) [-850.911] (-858.383) (-864.451) * (-861.245) [-854.420] (-862.333) (-857.396) -- 0:00:20 Average standard deviation of split frequencies: 0.007368 901000 -- [-857.464] (-858.975) (-860.816) (-856.754) * [-860.622] (-871.469) (-857.383) (-861.900) -- 0:00:19 902000 -- [-855.313] (-862.544) (-860.838) (-857.996) * (-867.274) [-857.397] (-858.533) (-857.471) -- 0:00:19 903000 -- (-854.301) [-859.806] (-859.000) (-862.237) * (-860.762) (-859.479) (-860.970) [-856.353] -- 0:00:19 904000 -- (-860.529) (-857.108) (-858.672) [-860.027] * [-856.147] (-860.510) (-856.525) (-863.241) -- 0:00:19 905000 -- [-858.650] (-861.089) (-854.155) (-861.220) * (-857.924) [-855.225] (-864.084) (-865.254) -- 0:00:19 Average standard deviation of split frequencies: 0.007805 906000 -- (-863.270) [-857.041] (-860.970) (-862.926) * (-860.132) (-862.551) (-859.314) [-857.930] -- 0:00:18 907000 -- (-862.073) (-859.686) [-859.655] (-861.808) * [-855.699] (-867.138) (-862.923) (-867.134) -- 0:00:18 908000 -- (-859.912) (-861.913) [-855.003] (-857.817) * [-859.900] (-862.826) (-860.751) (-856.150) -- 0:00:18 909000 -- (-860.254) (-861.977) (-868.963) [-857.735] * [-855.471] (-861.934) (-862.942) (-860.362) -- 0:00:18 910000 -- (-853.291) (-860.910) [-854.959] (-856.412) * (-852.890) (-865.535) (-865.268) [-857.090] -- 0:00:18 Average standard deviation of split frequencies: 0.007924 911000 -- (-861.910) [-857.278] (-855.425) (-856.600) * [-857.117] (-856.280) (-862.829) (-860.334) -- 0:00:17 912000 -- (-858.844) [-862.017] (-855.902) (-860.306) * (-860.330) (-857.298) (-863.465) [-854.956] -- 0:00:17 913000 -- (-856.971) (-858.019) (-857.994) [-855.642] * (-853.815) (-856.386) (-855.843) [-856.830] -- 0:00:17 914000 -- (-861.730) [-852.066] (-862.573) (-856.080) * (-858.048) (-860.808) (-858.925) [-860.212] -- 0:00:17 915000 -- (-861.228) [-860.111] (-861.520) (-856.754) * (-867.503) (-855.937) [-859.066] (-856.104) -- 0:00:17 Average standard deviation of split frequencies: 0.007878 916000 -- (-857.086) (-860.569) [-861.275] (-860.154) * (-859.119) [-858.641] (-859.085) (-860.723) -- 0:00:16 917000 -- (-860.684) (-860.266) [-864.594] (-854.203) * [-861.721] (-859.168) (-867.513) (-857.429) -- 0:00:16 918000 -- [-866.153] (-866.163) (-859.379) (-858.205) * (-859.212) (-856.204) (-861.028) [-853.518] -- 0:00:16 919000 -- (-860.835) (-863.053) [-857.750] (-860.991) * (-861.722) (-858.333) (-863.485) [-866.826] -- 0:00:16 920000 -- (-859.172) (-863.473) (-860.583) [-852.659] * (-858.284) (-853.887) (-862.385) [-867.061] -- 0:00:16 Average standard deviation of split frequencies: 0.007917 921000 -- (-862.036) (-866.642) (-859.677) [-851.463] * (-868.641) (-859.293) (-862.215) [-863.902] -- 0:00:15 922000 -- (-863.461) (-861.522) (-866.236) [-851.769] * (-865.962) (-855.461) [-858.320] (-865.811) -- 0:00:15 923000 -- (-860.097) [-861.740] (-864.394) (-854.523) * [-861.194] (-853.569) (-856.429) (-858.218) -- 0:00:15 924000 -- (-861.458) (-868.032) [-856.987] (-859.770) * (-857.162) [-857.818] (-870.802) (-860.286) -- 0:00:15 925000 -- (-860.883) (-860.769) [-858.795] (-867.359) * (-861.474) (-860.421) (-868.252) [-857.435] -- 0:00:15 Average standard deviation of split frequencies: 0.008028 926000 -- (-867.360) (-856.404) (-857.506) [-857.388] * (-863.278) [-857.958] (-865.841) (-863.658) -- 0:00:14 927000 -- (-860.795) (-859.240) (-865.593) [-859.328] * [-859.159] (-854.974) (-852.727) (-867.017) -- 0:00:14 928000 -- (-860.453) (-869.833) (-853.807) [-860.761] * (-865.714) (-859.025) (-863.277) [-866.459] -- 0:00:14 929000 -- (-856.225) (-860.169) [-857.944] (-866.287) * [-859.817] (-854.887) (-857.999) (-869.748) -- 0:00:14 930000 -- (-856.083) (-860.632) [-862.340] (-862.421) * [-859.461] (-865.271) (-860.743) (-867.088) -- 0:00:14 Average standard deviation of split frequencies: 0.008143 931000 -- (-852.698) [-862.943] (-861.721) (-863.944) * [-859.526] (-857.875) (-865.309) (-866.814) -- 0:00:13 932000 -- [-853.940] (-861.499) (-872.051) (-856.363) * [-866.647] (-864.065) (-859.801) (-861.243) -- 0:00:13 933000 -- (-857.047) [-858.872] (-856.554) (-864.519) * (-857.843) (-876.081) [-857.423] (-860.594) -- 0:00:13 934000 -- (-860.418) (-863.759) (-856.908) [-859.412] * (-861.124) (-864.827) (-858.500) [-859.313] -- 0:00:13 935000 -- (-860.914) (-855.029) (-857.934) [-856.869] * [-863.594] (-857.535) (-864.359) (-869.058) -- 0:00:13 Average standard deviation of split frequencies: 0.007516 936000 -- (-861.796) (-866.608) [-859.517] (-856.507) * (-861.747) [-860.344] (-858.895) (-859.157) -- 0:00:12 937000 -- (-858.930) (-862.089) (-857.740) [-862.544] * (-863.254) (-865.382) [-861.349] (-854.803) -- 0:00:12 938000 -- (-856.064) (-871.336) [-859.249] (-860.593) * (-869.484) (-859.730) (-871.856) [-864.428] -- 0:00:12 939000 -- (-855.010) (-854.952) [-853.059] (-876.917) * (-861.218) [-856.768] (-862.908) (-860.474) -- 0:00:12 940000 -- [-860.420] (-856.655) (-854.045) (-860.736) * [-860.283] (-864.612) (-864.411) (-864.966) -- 0:00:12 Average standard deviation of split frequencies: 0.007633 941000 -- (-854.859) (-859.120) [-863.119] (-860.900) * (-862.306) (-858.590) (-866.231) [-858.799] -- 0:00:11 942000 -- (-859.053) (-860.274) (-870.242) [-861.274] * [-862.281] (-856.653) (-859.137) (-863.994) -- 0:00:11 943000 -- (-864.999) (-853.141) (-865.902) [-861.267] * (-856.896) (-864.139) [-863.248] (-863.828) -- 0:00:11 944000 -- (-856.436) (-861.946) (-860.507) [-853.224] * [-861.286] (-856.415) (-869.154) (-856.010) -- 0:00:11 945000 -- (-861.644) [-856.319] (-858.315) (-856.719) * [-862.764] (-859.928) (-853.564) (-865.591) -- 0:00:11 Average standard deviation of split frequencies: 0.007551 946000 -- (-861.857) (-858.498) [-853.818] (-857.955) * (-863.932) [-851.161] (-857.798) (-863.444) -- 0:00:10 947000 -- (-856.803) (-858.578) (-856.540) [-862.318] * (-858.953) [-859.195] (-864.184) (-867.945) -- 0:00:10 948000 -- (-858.906) (-858.408) (-862.925) [-855.008] * [-862.963] (-856.311) (-860.359) (-858.223) -- 0:00:10 949000 -- [-869.224] (-861.566) (-862.649) (-861.937) * (-855.264) [-860.104] (-871.272) (-858.105) -- 0:00:10 950000 -- [-857.353] (-865.683) (-864.204) (-858.758) * [-852.833] (-860.824) (-864.179) (-862.470) -- 0:00:10 Average standard deviation of split frequencies: 0.007896 951000 -- (-859.358) (-863.351) [-853.594] (-857.328) * (-859.018) (-856.538) (-866.558) [-860.290] -- 0:00:09 952000 -- [-865.939] (-862.191) (-857.673) (-858.148) * (-857.404) [-855.036] (-863.725) (-855.883) -- 0:00:09 953000 -- [-856.160] (-858.980) (-855.735) (-865.773) * (-861.398) (-858.305) [-854.918] (-862.448) -- 0:00:09 954000 -- (-859.978) (-855.352) (-861.437) [-863.355] * [-860.263] (-862.383) (-854.866) (-856.439) -- 0:00:09 955000 -- (-864.432) (-860.607) (-862.444) [-866.376] * (-858.578) [-864.325] (-855.157) (-860.357) -- 0:00:09 Average standard deviation of split frequencies: 0.007321 956000 -- (-854.962) (-866.026) (-857.915) [-861.317] * [-854.901] (-858.626) (-857.174) (-860.682) -- 0:00:08 957000 -- (-862.346) (-864.989) [-853.918] (-859.143) * (-860.197) (-856.685) [-860.445] (-868.855) -- 0:00:08 958000 -- (-861.427) (-855.410) [-855.736] (-857.605) * (-855.590) [-853.893] (-858.942) (-859.885) -- 0:00:08 959000 -- [-857.724] (-856.782) (-862.156) (-861.376) * (-856.733) (-863.779) [-854.538] (-859.216) -- 0:00:08 960000 -- (-860.984) (-859.392) (-869.528) [-858.126] * [-857.626] (-876.977) (-856.230) (-860.337) -- 0:00:08 Average standard deviation of split frequencies: 0.007663 961000 -- (-863.804) (-859.173) (-863.765) [-858.147] * (-856.494) (-866.950) [-853.589] (-855.795) -- 0:00:07 962000 -- (-859.237) [-856.704] (-865.522) (-869.185) * (-858.437) [-865.563] (-858.004) (-859.254) -- 0:00:07 963000 -- [-859.147] (-857.945) (-861.190) (-858.480) * (-860.376) (-873.360) [-855.887] (-862.901) -- 0:00:07 964000 -- (-864.038) (-856.943) (-870.872) [-852.903] * (-854.569) (-869.023) (-862.382) [-855.806] -- 0:00:07 965000 -- (-864.297) (-859.717) (-862.223) [-854.994] * (-858.165) (-861.668) (-862.181) [-859.061] -- 0:00:07 Average standard deviation of split frequencies: 0.007020 966000 -- [-861.929] (-862.293) (-870.447) (-857.974) * [-863.278] (-871.681) (-872.211) (-853.659) -- 0:00:06 967000 -- (-854.481) (-858.251) (-857.646) [-854.778] * [-861.128] (-859.026) (-858.263) (-863.332) -- 0:00:06 968000 -- (-858.976) [-859.234] (-859.777) (-855.047) * (-862.042) (-861.409) (-862.097) [-856.177] -- 0:00:06 969000 -- (-862.789) [-860.843] (-858.125) (-856.629) * (-861.144) [-852.201] (-861.097) (-863.336) -- 0:00:06 970000 -- [-855.425] (-863.338) (-861.236) (-856.152) * [-854.975] (-863.603) (-855.854) (-859.904) -- 0:00:06 Average standard deviation of split frequencies: 0.007061 971000 -- (-863.457) [-861.245] (-871.015) (-858.980) * (-862.512) (-860.401) (-856.395) [-860.121] -- 0:00:05 972000 -- (-860.823) (-861.151) [-860.551] (-868.324) * (-860.225) (-862.786) (-863.647) [-861.409] -- 0:00:05 973000 -- (-859.450) (-858.229) [-858.140] (-859.147) * [-857.116] (-855.488) (-864.388) (-862.798) -- 0:00:05 974000 -- (-857.373) [-853.827] (-854.499) (-864.337) * [-854.927] (-857.665) (-867.468) (-864.371) -- 0:00:05 975000 -- (-858.619) (-861.713) [-859.205] (-862.665) * (-861.515) (-860.284) [-861.845] (-861.541) -- 0:00:05 Average standard deviation of split frequencies: 0.006799 976000 -- (-855.797) (-861.132) [-853.785] (-861.620) * (-859.080) (-861.722) [-858.069] (-863.961) -- 0:00:04 977000 -- [-857.319] (-859.691) (-864.270) (-860.407) * (-868.291) [-854.850] (-862.566) (-863.315) -- 0:00:04 978000 -- (-859.782) (-861.186) (-862.492) [-856.788] * (-858.391) [-854.037] (-854.367) (-863.177) -- 0:00:04 979000 -- (-860.391) (-858.245) [-858.575] (-860.710) * (-860.200) (-858.407) [-854.452] (-858.353) -- 0:00:04 980000 -- (-861.163) (-866.162) [-857.016] (-860.505) * [-861.028] (-861.912) (-858.407) (-855.387) -- 0:00:04 Average standard deviation of split frequencies: 0.006915 981000 -- (-856.464) [-858.071] (-859.166) (-857.933) * (-862.855) (-863.299) [-864.445] (-859.386) -- 0:00:03 982000 -- (-861.144) (-858.402) [-857.125] (-866.370) * [-858.830] (-855.433) (-861.030) (-861.777) -- 0:00:03 983000 -- (-856.145) [-861.352] (-858.471) (-858.265) * (-854.562) [-855.714] (-858.001) (-856.221) -- 0:00:03 984000 -- (-858.142) (-860.703) [-858.691] (-856.552) * (-866.857) [-855.007] (-865.739) (-858.079) -- 0:00:03 985000 -- (-864.606) (-854.670) [-860.716] (-862.219) * (-863.923) [-858.450] (-855.945) (-865.192) -- 0:00:03 Average standard deviation of split frequencies: 0.007392 986000 -- (-861.354) (-856.877) (-863.293) [-858.780] * (-862.217) (-873.741) [-860.774] (-862.862) -- 0:00:02 987000 -- [-858.927] (-873.301) (-859.675) (-854.894) * (-860.633) [-853.657] (-862.226) (-863.990) -- 0:00:02 988000 -- (-858.271) (-866.999) [-858.516] (-858.699) * (-856.863) (-861.660) (-860.116) [-858.122] -- 0:00:02 989000 -- [-868.735] (-866.351) (-855.802) (-855.727) * (-870.087) [-858.196] (-865.062) (-859.666) -- 0:00:02 990000 -- (-858.636) (-862.552) (-862.062) [-865.220] * (-869.244) [-861.887] (-870.260) (-866.945) -- 0:00:02 Average standard deviation of split frequencies: 0.007650 991000 -- (-857.690) (-863.350) [-858.815] (-867.162) * (-863.264) (-861.547) [-856.749] (-861.691) -- 0:00:01 992000 -- (-859.893) (-859.506) (-860.673) [-862.065] * [-860.506] (-867.221) (-857.378) (-864.530) -- 0:00:01 993000 -- (-856.650) [-853.519] (-865.649) (-864.819) * [-864.307] (-861.723) (-861.316) (-865.267) -- 0:00:01 994000 -- (-861.242) (-862.472) (-858.103) [-855.664] * (-868.174) (-861.178) (-863.835) [-855.748] -- 0:00:01 995000 -- (-858.625) (-862.295) [-858.060] (-863.581) * (-862.341) (-868.038) [-855.602] (-861.602) -- 0:00:01 Average standard deviation of split frequencies: 0.007900 996000 -- (-862.039) [-862.394] (-859.396) (-861.146) * (-858.151) (-857.447) [-857.769] (-865.828) -- 0:00:00 997000 -- [-860.636] (-853.892) (-862.105) (-857.146) * (-862.248) (-868.782) (-864.006) [-858.543] -- 0:00:00 998000 -- [-859.442] (-855.898) (-866.363) (-863.885) * (-866.209) (-858.722) [-863.236] (-857.532) -- 0:00:00 999000 -- (-857.076) [-858.726] (-858.985) (-858.171) * [-858.126] (-858.448) (-858.910) (-867.067) -- 0:00:00 1000000 -- [-865.331] (-864.222) (-855.848) (-858.095) * (-860.822) (-854.345) (-866.359) [-866.494] -- 0:00:00 Average standard deviation of split frequencies: 0.007682 Analysis completed in 3 mins 20 seconds Analysis used 199.43 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -849.06 Likelihood of best state for "cold" chain of run 2 was -849.10 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 67.4 % ( 61 %) Dirichlet(Revmat{all}) 82.3 % ( 73 %) Slider(Revmat{all}) 32.9 % ( 36 %) Dirichlet(Pi{all}) 33.7 % ( 28 %) Slider(Pi{all}) 79.8 % ( 65 %) Multiplier(Alpha{1,2}) 74.9 % ( 53 %) Multiplier(Alpha{3}) 90.4 % ( 92 %) Slider(Pinvar{all}) 37.0 % ( 43 %) ExtSPR(Tau{all},V{all}) 24.9 % ( 33 %) ExtTBR(Tau{all},V{all}) 40.1 % ( 41 %) NNI(Tau{all},V{all}) 40.3 % ( 34 %) ParsSPR(Tau{all},V{all}) 27.0 % ( 33 %) Multiplier(V{all}) 51.7 % ( 54 %) Nodeslider(V{all}) 26.5 % ( 32 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 68.7 % ( 65 %) Dirichlet(Revmat{all}) 82.5 % ( 73 %) Slider(Revmat{all}) 32.5 % ( 31 %) Dirichlet(Pi{all}) 34.1 % ( 30 %) Slider(Pi{all}) 79.8 % ( 70 %) Multiplier(Alpha{1,2}) 74.4 % ( 52 %) Multiplier(Alpha{3}) 89.7 % ( 83 %) Slider(Pinvar{all}) 37.4 % ( 33 %) ExtSPR(Tau{all},V{all}) 25.1 % ( 20 %) ExtTBR(Tau{all},V{all}) 40.1 % ( 41 %) NNI(Tau{all},V{all}) 40.6 % ( 31 %) ParsSPR(Tau{all},V{all}) 27.1 % ( 28 %) Multiplier(V{all}) 51.5 % ( 55 %) Nodeslider(V{all}) 26.3 % ( 27 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.77 0.57 0.41 2 | 166930 0.78 0.60 3 | 165911 166218 0.80 4 | 166835 167228 166878 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.76 0.57 0.41 2 | 166324 0.78 0.60 3 | 167306 166890 0.80 4 | 166350 166044 167086 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -856.74 | 2 | | 1 1 1 1 | | 21 2 2 1 2 *1 | | 2 1 2 11 22 1 1 11 2 | | 2 2 21 1 112 2 1 2 2 1** | |2 1 2 2 11 2 1 21 1 * 2 | | 2 * 1 2 1 *1 1 2 2 1 | |1 * 1 2* 1 1 122 1 1 1 | | 1 * 2 22 2 1 1 2 1 2 2 12 1| | 2 21 1 1 1 2 | | 2 1 2 2 2 | | 1 2 2| | | | 2 | | 2 2 2 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -861.02 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -854.85 -865.95 2 -854.98 -867.89 -------------------------------------- TOTAL -854.92 -867.33 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.092875 0.000357 0.057565 0.129556 0.091363 1158.92 1329.96 1.000 r(A<->C){all} 0.079939 0.001727 0.011718 0.163824 0.073825 606.41 715.96 1.000 r(A<->G){all} 0.255574 0.005736 0.128504 0.424591 0.251281 468.36 501.63 1.000 r(A<->T){all} 0.083112 0.001599 0.014191 0.158056 0.077325 525.11 600.48 1.000 r(C<->G){all} 0.043157 0.001528 0.000024 0.121421 0.032054 497.06 542.15 1.003 r(C<->T){all} 0.391477 0.006492 0.240316 0.555939 0.388057 573.48 616.43 1.000 r(G<->T){all} 0.146741 0.003476 0.042324 0.261820 0.138604 524.41 587.45 1.000 pi(A){all} 0.295688 0.000441 0.255827 0.337544 0.295302 1263.61 1263.85 1.001 pi(C){all} 0.225913 0.000350 0.190637 0.263648 0.225945 1191.64 1250.30 1.001 pi(G){all} 0.194070 0.000316 0.159174 0.228003 0.193503 1287.40 1337.52 1.000 pi(T){all} 0.284328 0.000435 0.246031 0.325568 0.283826 1244.71 1255.44 1.000 alpha{1,2} 0.738954 0.719907 0.000020 2.432164 0.456265 1143.14 1143.46 1.000 alpha{3} 1.323106 1.254616 0.000264 3.480914 1.015594 983.47 1194.93 1.000 pinvar{all} 0.373306 0.043269 0.000543 0.705739 0.370966 563.58 755.07 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C2 3 -- C3 4 -- C4 5 -- C5 6 -- C6 7 -- C7 8 -- C8 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition -------------- 1 -- .******* 2 -- .*...... 3 -- ..*..... 4 -- ...*.... 5 -- ....*... 6 -- .....*.. 7 -- ......*. 8 -- .......* 9 -- .**..... 10 -- .....**. 11 -- .**..**. 12 -- ....*..* 13 -- ...**..* 14 -- ...**... 15 -- .**.**** 16 -- ...*...* 17 -- .**..*** 18 -- .***.**. 19 -- .**.***. 20 -- .******. 21 -- .***.*** -------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 9 3002 1.000000 0.000000 1.000000 1.000000 2 10 3002 1.000000 0.000000 1.000000 1.000000 2 11 2960 0.986009 0.004711 0.982678 0.989340 2 12 623 0.207528 0.002355 0.205863 0.209194 2 13 618 0.205863 0.002827 0.203864 0.207861 2 14 607 0.202199 0.025910 0.183877 0.220520 2 15 605 0.201532 0.005182 0.197868 0.205197 2 16 604 0.201199 0.001884 0.199867 0.202532 2 17 594 0.197868 0.016959 0.185876 0.209860 2 18 594 0.197868 0.015075 0.187209 0.208528 2 19 587 0.195536 0.002355 0.193871 0.197202 2 20 580 0.193205 0.012248 0.184544 0.201865 2 21 556 0.185210 0.010364 0.177881 0.192538 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.005171 0.000010 0.000475 0.011156 0.004617 1.000 2 length{all}[2] 0.010685 0.000027 0.001447 0.020368 0.009953 1.000 2 length{all}[3] 0.011949 0.000030 0.002882 0.022695 0.011094 1.002 2 length{all}[4] 0.001679 0.000003 0.000000 0.005140 0.001158 1.000 2 length{all}[5] 0.001728 0.000003 0.000001 0.005133 0.001206 1.000 2 length{all}[6] 0.001753 0.000003 0.000000 0.005336 0.001198 1.000 2 length{all}[7] 0.001697 0.000003 0.000001 0.005085 0.001180 1.000 2 length{all}[8] 0.001750 0.000003 0.000000 0.005303 0.001142 1.000 2 length{all}[9] 0.023198 0.000070 0.008642 0.039449 0.022201 1.000 2 length{all}[10] 0.020517 0.000056 0.008117 0.036541 0.019448 1.000 2 length{all}[11] 0.009348 0.000024 0.001270 0.019420 0.008537 1.000 2 length{all}[12] 0.001805 0.000003 0.000002 0.005524 0.001245 1.002 2 length{all}[13] 0.001759 0.000003 0.000000 0.005267 0.001209 1.000 2 length{all}[14] 0.001671 0.000003 0.000002 0.005341 0.001158 0.998 2 length{all}[15] 0.001761 0.000004 0.000000 0.005445 0.001194 0.998 2 length{all}[16] 0.001778 0.000003 0.000001 0.005491 0.001219 1.000 2 length{all}[17] 0.001757 0.000003 0.000002 0.005302 0.001149 1.000 2 length{all}[18] 0.001678 0.000003 0.000000 0.004745 0.001228 0.998 2 length{all}[19] 0.001844 0.000003 0.000001 0.005764 0.001314 0.999 2 length{all}[20] 0.001782 0.000004 0.000000 0.005332 0.001198 1.008 2 length{all}[21] 0.001726 0.000003 0.000002 0.004987 0.001209 0.998 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.007682 Maximum standard deviation of split frequencies = 0.025910 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.008 Clade credibility values: /------------------------------------------------------------------------ C1 (1) | |------------------------------------------------------------------------ C4 (4) | |------------------------------------------------------------------------ C5 (5) | +------------------------------------------------------------------------ C8 (8) | | /------------------------ C2 (2) | /----------100----------+ | | \------------------------ C3 (3) \-----------99----------+ | /------------------------ C6 (6) \----------100----------+ \------------------------ C7 (7) Phylogram (based on average branch lengths): /-------- C1 (1) | |-- C4 (4) | |-- C5 (5) | +-- C8 (8) | | /----------------- C2 (2) | /-------------------------------------+ | | \------------------- C3 (3) \--------------+ | /-- C6 (6) \--------------------------------+ \-- C7 (7) |-------| 0.005 expected changes per site Calculating tree probabilities... Credible sets of trees (46 trees sampled): 50 % credible set contains 8 trees 90 % credible set contains 14 trees 95 % credible set contains 15 trees 99 % credible set contains 21 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C4,C5,C8,((C2,C3),(C6,C7)))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **8** sequences, **153** codons, and **1** partitions from `/data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/results/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/results/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/results/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -847.97, AIC-c = 1734.14 (19 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.083 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 0.786 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 78 | 1 | 2.813 | 29.036 | Pos. posterior = 0.9511 | | 82 | 1 | 2.293 | 18.830 | Pos. posterior = 0.9197 | ---- ## FUBAR inferred 2 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.13 are expected to be false positives (95% confidence interval of 0-1 )
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.05 sec, SCORE=1000, Nseq=8, Len=153 LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG 175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSAAPTTYRASQAAKVLIHTTEKVTLNNQRTHSYTKWS 183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSAAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG *********:*** :***************:************:*****. LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS 175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPKPTAVAIPTFPYEPRSEKPGFGS 183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETTKPPRPTAIAIPTFPYEPRSEKPGFGS SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS ***********************:***.***.****.*** ********* LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS 175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWST 183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHQTFAVTTFRWST SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS ***********:***.**** *****************:*******:**: LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 PLN 175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 PSN 183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 PSN SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 PSN TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 PSN TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 PSN TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 PSN TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 PSN * *
>LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTCTTAGCTTTTCCTCTTCTGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTATACCACTGAAAAAGTCACTCTAAATAACCAGAGAACATACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAAGACCAACAGCCTTTGCCATTCCAACATTTCCATACGAGCCTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGATTATGGCCGTATTGAGTATGAGAGTATCTTGTGTGCTGCATTTGAGCATGTCATTAATGACATTGCAAAAATTGCTGCTCAATTGGCTCAAACACAGCGTAGACATCATACTTTTGCTGTGACGACTTTCAAGTGGTCTTCTCCTTTAAAT >175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTCTTAGCTTTTCTTCTGCAGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTCATACCACTGAAAAAGTCACTCTAAATAACCAGAGAACACACTCATACACAAAATGGAGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAAAACCAACAGCCGTTGCTATTCCAACATTCCCATACGAGCCTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGACTACGGCCGTATTGAGTATGAGAGTATCTTGTGTGCTGCATTTGAGCATGTCAGTAATGACATTGCAAAAATTGCTGCTCAATTGGCTCAGACACAGCGACGACATCACACTTTCGCTGTGACGACTTTTAAGTGGTCTACTCCTTCAAAT >183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTCTTAGCTTTTCTTCTGCAGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTATACCACTGAAAAAGTCACTCTAAATAACCAGAGAACACACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAACCAAACCGCCAAGACCCACAGCCATTGCTATTCCAACATTCCCATACGAGCCTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGACTATGGCCGTATTGAGTATGAGAGTATCTTGTGTGCTGCATTTGAGCATGTCAGTAATGACATTGCAAAAATTGCTGCTCAATTGGCTCAGACACAGCGACGACATCAGACTTTCGCTGTGACTACTTTTAGGTGGTCTACTCCTTCAAAT >SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTCTTAGCTTTTCCTCTTCTGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTATACCACTGAAAAAGTCACTCTAAATAACCAGAGAACATACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAAGACCAACAGCCTTTGCTATTCCAACATTTCCATACGAGCCTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGATTATGGCCGTATTGAGTATGAGAGTATCTTGTGTGCTGCATTTGAGCATGTCATTAATGACATTGCAAAAATTGCTGCTCAATTGGCTCAAACACAGCGTAGACATCATACTTTTGCTGTGACGACTTTCAAGTGGTCTTCTCCTTCAAAT >TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTCTTAGCTTTTCCTCTTCTGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTATACCACTGAAAAAGTCACTCTAAATAACCAGAGAACATACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAAGACCAACAGCCTTTGCTATTCCAACATTTCCATACGAGCCTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGATTATGGCCGTATTGAGTATGAGAGTATCTTGTGTGCTGCATTTGAGCATGTCATTAATGACATTGCAAAAATTGCTGCTCAATTGGCTCAAACACAGCGTAGACATCATACTTTTGCTGTGACGACTTTCAAGTGGTCTTCTCCTTCAAAT >TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTTTTAGCTTTTCCTATTCTGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTACACCACTGAAAAAGTCACTCTAAATAACCAGAGAACACACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAGAACCAACAGCCATTGCTATTCCAACAGTCCCATACGAGCTTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGATTATGGCCGTATTGAGTATGAGAGTGTCTTGTGTGCTGTATTTGAGCATGTCAGTAATGACATTGCAAAAATTGCTGCCCAATTGGCTCAAACACAGCGTAGACATCATACTTTTGCTGTGACGACTTTCAAGTGGTCTTCTCCTTCAAAT >TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTTTTAGCTTTTCCTATTCTGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTACACCACTGAAAAAGTCACTCTAAATAACCAGAGAACACACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAGAACCAACAGCCATTGCTATTCCAACAGTCCCATACGAGCTTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGATTATGGCCGTATTGAGTATGAGAGTGTCTTGTGTGCTGTATTTGAGCATGTCAGTAATGACATTGCAAAAATTGCTGCCCAATTGGCTCAAACACAGCGTAGACATCATACTTTTGCTGTGACGACTTTCAAGTGGTCTTCTCCTTCAAAT >TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 ATGAAATTGTTACTGCTTCTTAGTATTCTTAGCTTTTCCTCTTCTGCCCCAACTACCTATCGGGCATCTCAAGCTGCTAAAGTACTAATTTATACCACTGAAAAAGTCACTCTAAATAACCAGAGAACATACTCATACACAAAATGGGGTGTGTGCTCCACAGGATGGAACACATACACGAACACAATGGTTGTGGTTAATGGTAGGTGGGTTGAAACAGCCAAACCGCCAAGACCAACAGCCTTTGCTATTCCAACATTTCCATACGAGCCTAGAAGTGAAAAACCAGGTTTTGGTTCTCACTTTGATTATGGCCGTATTGAGTATGAGAGTATCTTGTGTGCTGCATTTGAGCATGTCATTAATGACATTGCAAAAATTGCTGCTCAATTGGCTCAAACACAGCGTAGACATCATACTTTTGCTGTGACGACTTTCAAGTGGTCTTCTCCTTCAAAT
>LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS PLN >175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSAAPTTYRASQAAKVLIHTTEKVTLNNQRTHSYTKWS VCSTGWNTYTNTMVVVNGRWVETAKPPKPTAVAIPTFPYEPRSEKPGFGS HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWST PSN >183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSAAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG VCSTGWNTYTNTMVVVNGRWVETTKPPRPTAIAIPTFPYEPRSEKPGFGS HFDYGRIEYESILCAAFEHVSNDIAKIAAQLAQTQRRHQTFAVTTFRWST PSN >SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS PSN >TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS PSN >TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS PSN >TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSIFSFSYSAPTTYRASQAAKVLIYTTEKVTLNNQRTHSYTKWG VCSTGWNTYTNTMVVVNGRWVETAKPPEPTAIAIPTVPYELRSEKPGFGS HFDYGRIEYESVLCAVFEHVSNDIAKIAAQLAQTQRRHHTFAVTTFKWSS PSN >TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 MKLLLLLSILSFSSSAPTTYRASQAAKVLIYTTEKVTLNNQRTYSYTKWG VCSTGWNTYTNTMVVVNGRWVETAKPPRPTAFAIPTFPYEPRSEKPGFGS HFDYGRIEYESILCAAFEHVINDIAKIAAQLAQTQRRHHTFAVTTFKWSS PSN
Reading sequence file /data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/fasta/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1 Found 8 sequences of length 459 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 3.0% Found 24 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 4 Calculating analytical mean and variance Doing permutation test for PHI Doing permutation test for NSS Doing Permutation test for MAXCHI Writing alignment of polymorphic unambig sites to: Phi.poly.sites Window size is 22 polymorphic sites **p-Value(s)** ---------- NSS: 1.05e-01 (1000 permutations) Max Chi^2: 3.47e-01 (1000 permutations) PHI (Permutation): 9.20e-02 (1000 permutations) PHI (Normal): 4.66e-02
#NEXUS [ID: 5175601111] begin taxa; dimensions ntax=8; taxlabels LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10 183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10 SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10 TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10 TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 ; end; begin trees; translate 1 LSH5A_NS7b_AFU92101_1_2005_12_Hong_Kong_Bat_Bat_coronavirus_HKU10, 2 175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10, 3 183A_NS7b_AFU92110_1_2005_10_China_Bat_Bat_coronavirus_HKU10, 4 SL12A_NS7b_AFU92083_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10, 5 TLC1310A_NS7b_AFU92092_1_2006_10_Hong_Kong_Bat_Bat_coronavirus_HKU10, 6 TLC1343A_NS7b_AFU92128_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10, 7 TLC1347A_NS7b_AFU92137_1_2010_08_Hong_Kong_Bat_Bat_coronavirus_HKU10, 8 TT3A_NS7b_AFU92075_1_2006_12_Hong_Kong_Bat_Bat_coronavirus_HKU10 ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:4.617026e-03,4:1.158163e-03,5:1.205889e-03,8:1.142244e-03,((2:9.953314e-03,3:1.109382e-02)1.000:2.220128e-02,(6:1.198174e-03,7:1.179639e-03)1.000:1.944816e-02)0.986:8.536948e-03); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:4.617026e-03,4:1.158163e-03,5:1.205889e-03,8:1.142244e-03,((2:9.953314e-03,3:1.109382e-02):2.220128e-02,(6:1.198174e-03,7:1.179639e-03):1.944816e-02):8.536948e-03); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -854.97 -866.31 2 -854.96 -866.16 -------------------------------------- TOTAL -854.97 -866.24 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.093203 0.000358 0.058858 0.131155 0.090837 1347.16 1424.08 1.000 r(A<->C){all} 0.082698 0.001753 0.015341 0.167786 0.076230 677.63 754.93 1.002 r(A<->G){all} 0.253154 0.005514 0.122308 0.407673 0.247810 340.57 371.16 1.000 r(A<->T){all} 0.082169 0.001618 0.014997 0.161858 0.075938 595.54 642.39 1.001 r(C<->G){all} 0.043326 0.001472 0.000018 0.118620 0.032519 467.44 515.63 1.003 r(C<->T){all} 0.390746 0.006680 0.231735 0.543070 0.388783 454.16 539.93 1.000 r(G<->T){all} 0.147908 0.003503 0.037087 0.260888 0.141226 590.27 620.87 1.007 pi(A){all} 0.295606 0.000421 0.256404 0.335086 0.294951 1199.82 1325.62 1.000 pi(C){all} 0.225250 0.000345 0.189409 0.262090 0.224890 1248.34 1254.17 1.000 pi(G){all} 0.193895 0.000330 0.157420 0.228435 0.193361 980.79 1184.40 1.000 pi(T){all} 0.285249 0.000404 0.248145 0.325299 0.284923 1177.44 1200.22 1.000 alpha{1,2} 0.703206 0.615702 0.000198 2.241400 0.441514 1152.55 1212.30 1.000 alpha{3} 1.324228 1.223212 0.000646 3.485764 1.019498 1202.04 1314.42 1.000 pinvar{all} 0.371197 0.041543 0.004650 0.708667 0.368601 727.96 808.22 1.001 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C4,C5,C8,((C2,C3),(C6,C7)))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **8** sequences, **153** codons, and **1** partitions from `/data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/results/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/results/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result/original_alignment/fubar/results/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1/175A_NS7b_AFU92119_1_2005_10_China_Bat_Bat_coronavirus_HKU10.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -847.97, AIC-c = 1734.14 (19 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.083 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 0.786 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results | Codon | Partition | alpha | beta |Posterior prob for positive selection| |:--------------:|:--------------:|:--------------:|:--------------:|:-----------------------------------:| | 78 | 1 | 2.813 | 29.036 | Pos. posterior = 0.9511 | | 82 | 1 | 2.293 | 18.830 | Pos. posterior = 0.9197 | ---- ## FUBAR inferred 2 sites subject to diversifying positive selection at posterior probability >= 0.9 Of these, 0.13 are expected to be false positives (95% confidence interval of 0-1 )
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500