--- EXPERIMENT NOTES Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500 --- EXPERIMENT PROPERTIES --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -141.73 -152.56 2 -141.72 -154.91 -------------------------------------- TOTAL -141.73 -154.31 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.324707 0.022122 0.110791 0.607763 0.297195 1271.40 1386.20 1.000 r(A<->C){all} 0.063787 0.003933 0.000012 0.192315 0.044259 284.09 289.43 1.005 r(A<->G){all} 0.342964 0.017076 0.093315 0.591816 0.339198 93.45 133.24 1.010 r(A<->T){all} 0.077452 0.004119 0.000008 0.208162 0.062552 204.26 224.26 1.000 r(C<->G){all} 0.064250 0.003871 0.000002 0.184231 0.045722 194.15 214.29 1.000 r(C<->T){all} 0.352910 0.015936 0.117457 0.594664 0.345420 113.65 139.43 1.015 r(G<->T){all} 0.098637 0.004746 0.000317 0.234816 0.083536 183.80 253.12 1.010 pi(A){all} 0.241663 0.002641 0.144656 0.346215 0.239310 669.24 749.83 1.000 pi(C){all} 0.196624 0.002295 0.103435 0.284129 0.194183 645.99 762.79 1.004 pi(G){all} 0.276434 0.002825 0.182206 0.386350 0.274207 872.33 893.15 1.001 pi(T){all} 0.285279 0.003089 0.180605 0.394718 0.283671 821.25 855.12 1.001 alpha{1,2} 0.582281 0.528540 0.000224 1.982054 0.327552 780.91 827.42 1.001 alpha{3} 1.509678 1.261131 0.001708 3.829231 1.216155 1136.70 1168.23 1.000 pinvar{all} 0.325199 0.038307 0.001402 0.665188 0.312099 593.74 659.56 1.002 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. --- CODEML SUMMARY
-- Starting log on Wed Nov 09 22:46:01 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=11, Len=19 C1 GTTIDQSYLNECGVLVQLD C2 GTTIDQSYLNECGVLVQLD C3 SSTVDQSYLNECGVLVQLD C4 GTTIDQSYLNECGVLVQLD C5 STTIDQSYLNECGVLVQLD C6 GTTIDQSYLNECGVLVQLD C7 GATMDQSYLNECGVLVQLD C8 SSTVDQSYLNECGVLVQLD C9 SSTVDQSYLNECGVLVQLD C10 SFTVDQSYLNECGVLVQLD C11 SSTVDQSYLNECGVLVQLD . *:*************** -- Starting log on Wed Nov 09 22:47:03 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=11, Len=19 C1 GTTIDQSYLNECGVLVQLD C2 GTTIDQSYLNECGVLVQLD C3 SSTVDQSYLNECGVLVQLD C4 GTTIDQSYLNECGVLVQLD C5 STTIDQSYLNECGVLVQLD C6 GTTIDQSYLNECGVLVQLD C7 GATMDQSYLNECGVLVQLD C8 SSTVDQSYLNECGVLVQLD C9 SSTVDQSYLNECGVLVQLD C10 SFTVDQSYLNECGVLVQLD C11 SSTVDQSYLNECGVLVQLD . *:*************** -- Starting log on Wed Nov 09 22:46:01 GMT 2022 -- -- Iteration: /working_dir/input/2_modified/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result-- CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=11, Len=19 C1 GTTIDQSYLNECGVLVQLD C2 GTTIDQSYLNECGVLVQLD C3 SSTVDQSYLNECGVLVQLD C4 GTTIDQSYLNECGVLVQLD C5 STTIDQSYLNECGVLVQLD C6 GTTIDQSYLNECGVLVQLD C7 GATMDQSYLNECGVLVQLD C8 SSTVDQSYLNECGVLVQLD C9 SSTVDQSYLNECGVLVQLD C10 SFTVDQSYLNECGVLVQLD C11 SSTVDQSYLNECGVLVQLD . *:*************** -- Starting log on Thu Nov 10 01:14:35 GMT 2022 -- -- Iteration: /working_dir/pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/gapped_alignment/fubar,A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1-- MrBayes v3.2.6 x64 (Bayesian Analysis of Phylogeny) Distributed under the GNU General Public License Type "help" or "help <command>" for information on the commands that are available. Type "about" for authorship and general information about the program. Executing file "/data/mrbayes_input.nex" UNIX line termination Longest line length = 63 Parsing file Expecting NEXUS formatted file Reading data block Allocated taxon set Allocated matrix Defining new matrix with 11 taxa and 57 characters Missing data coded as ? Data matrix is interleaved Data is Dna Gaps coded as - Matching characters coded as . Taxon 1 -> C1 Taxon 2 -> C10 Taxon 3 -> C11 Taxon 4 -> C2 Taxon 5 -> C3 Taxon 6 -> C4 Taxon 7 -> C5 Taxon 8 -> C6 Taxon 9 -> C7 Taxon 10 -> C8 Taxon 11 -> C9 Successfully read matrix Setting default partition (does not divide up characters) Setting model defaults Seed (for generating default start values) = 1668042877 Setting output file names to "/data/mrbayes_input.nex.run<i>.<p|t>" Exiting data block Reading mrbayes block Setting autoclose to yes Setting nowarnings to yes Defining charset called 'first_pos' Defining charset called 'second_pos' Defining charset called 'third_pos' Defining partition called 'by_codon' Setting by_codon as the partition, dividing characters into 3 parts. Setting model defaults Seed (for generating default start values) = 72715673 Setting Nst to 6 for partition 1 Setting Nst to 6 for partition 2 Setting Nst to 6 for partition 3 Setting Rates to Invgamma for partition 1 Setting Rates to Invgamma for partition 2 Setting Rates to Invgamma for partition 3 Successfully set likelihood model parameters to all applicable data partitions Unlinking Setting number of generations to 1000000 Running Markov chain MCMC stamp = 7555419412 Seed = 2083565577 Swapseed = 1668042877 Model settings: Settings for partition 1 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 2 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Settings for partition 3 -- Datatype = DNA Nucmodel = 4by4 Nst = 6 Substitution rates, expressed as proportions of the rate sum, have a Dirichlet prior (1.00,1.00,1.00,1.00,1.00,1.00) Covarion = No # States = 4 State frequencies have a Dirichlet prior (1.00,1.00,1.00,1.00) Rates = Invgamma The distribution is approximated using 4 categories. Likelihood summarized over all rate categories in each generation. Shape parameter is exponentially distributed with parameter (1.00). Proportion of invariable sites is uniformly dist- ributed on the interval (0.00,1.00). Active parameters: Partition(s) Parameters 1 2 3 --------------------------- Revmat 1 1 1 Statefreq 2 2 2 Shape 3 3 4 Pinvar 5 5 5 Ratemultiplier 6 6 6 Topology 7 7 7 Brlens 8 8 8 --------------------------- Parameters can be linked or unlinked across partitions using 'link' and 'unlink' 1 -- Parameter = Revmat{all} Type = Rates of reversible rate matrix Prior = Dirichlet(1.00,1.00,1.00,1.00,1.00,1.00) Partitions = All 2 -- Parameter = Pi{all} Type = Stationary state frequencies Prior = Dirichlet Partitions = All 3 -- Parameter = Alpha{1,2} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partitions = 1 and 2 4 -- Parameter = Alpha{3} Type = Shape of scaled gamma distribution of site rates Prior = Exponential(1.00) Partition = 3 5 -- Parameter = Pinvar{all} Type = Proportion of invariable sites Prior = Uniform(0.00,1.00) Partitions = All 6 -- Parameter = Ratemultiplier{all} Type = Partition-specific rate multiplier Prior = Fixed(1.0) Partitions = All 7 -- Parameter = Tau{all} Type = Topology Prior = All topologies equally probable a priori Partitions = All Subparam. = V{all} 8 -- Parameter = V{all} Type = Branch lengths Prior = Unconstrained:GammaDir(1.0,0.1000,1.0,1.0) Partitions = All The MCMC sampler will use the following moves: With prob. Chain will use move 0.91 % Dirichlet(Revmat{all}) 0.91 % Slider(Revmat{all}) 0.91 % Dirichlet(Pi{all}) 0.91 % Slider(Pi{all}) 1.82 % Multiplier(Alpha{1,2}) 1.82 % Multiplier(Alpha{3}) 1.82 % Slider(Pinvar{all}) 9.09 % ExtSPR(Tau{all},V{all}) 9.09 % ExtTBR(Tau{all},V{all}) 9.09 % NNI(Tau{all},V{all}) 9.09 % ParsSPR(Tau{all},V{all}) 36.36 % Multiplier(V{all}) 12.73 % Nodeslider(V{all}) 5.45 % TLMultiplier(V{all}) Division 1 has 7 unique site patterns Division 2 has 5 unique site patterns Division 3 has 8 unique site patterns Initializing conditional likelihoods Using standard SSE likelihood calculator for division 1 (single-precision) Using standard SSE likelihood calculator for division 2 (single-precision) Using standard SSE likelihood calculator for division 3 (single-precision) Initializing invariable-site conditional likelihoods Initial log likelihoods and log prior probs for run 1: Chain 1 -- -221.274858 -- 38.695331 Chain 2 -- -236.714236 -- 38.695331 Chain 3 -- -238.321204 -- 38.695331 Chain 4 -- -230.065964 -- 38.695331 Initial log likelihoods and log prior probs for run 2: Chain 1 -- -219.731883 -- 38.695331 Chain 2 -- -223.208984 -- 38.695331 Chain 3 -- -208.351320 -- 38.695331 Chain 4 -- -214.867095 -- 38.695331 Using a relative burnin of 25.0 % for diagnostics Chain results (1000000 generations requested): 0 -- [-221.275] (-236.714) (-238.321) (-230.066) * [-219.732] (-223.209) (-208.351) (-214.867) 1000 -- [-149.820] (-151.632) (-155.219) (-149.258) * [-157.249] (-161.940) (-153.069) (-151.082) -- 0:00:00 2000 -- (-150.500) (-152.021) [-151.431] (-152.897) * (-152.234) (-147.676) [-149.220] (-153.881) -- 0:08:19 3000 -- (-154.176) [-148.768] (-157.425) (-144.017) * [-148.797] (-148.727) (-149.831) (-159.560) -- 0:05:32 4000 -- (-150.878) [-146.151] (-152.056) (-154.447) * (-145.455) (-156.536) [-150.724] (-142.932) -- 0:04:09 5000 -- (-151.773) (-157.317) (-149.290) [-150.237] * (-144.372) [-142.643] (-156.328) (-153.110) -- 0:03:19 Average standard deviation of split frequencies: 0.091357 6000 -- [-151.238] (-160.051) (-145.953) (-151.355) * [-144.599] (-152.955) (-159.241) (-148.244) -- 0:02:45 7000 -- [-144.141] (-151.777) (-149.072) (-150.790) * (-143.852) [-149.856] (-161.566) (-149.278) -- 0:02:21 8000 -- (-143.183) (-159.097) [-141.034] (-153.322) * (-147.648) [-143.471] (-159.333) (-152.012) -- 0:04:08 9000 -- (-151.950) (-155.544) [-145.737] (-166.354) * [-146.329] (-146.616) (-165.736) (-162.947) -- 0:03:40 10000 -- (-144.907) (-158.408) [-142.575] (-165.632) * (-150.870) (-143.100) (-159.609) [-151.405] -- 0:03:18 Average standard deviation of split frequencies: 0.082250 11000 -- (-143.187) (-160.521) [-152.129] (-165.220) * [-142.761] (-148.368) (-157.266) (-152.444) -- 0:02:59 12000 -- (-143.325) (-159.540) [-145.802] (-157.762) * [-139.439] (-153.655) (-159.797) (-154.154) -- 0:02:44 13000 -- (-152.453) (-161.282) [-150.696] (-158.829) * (-151.768) (-145.544) (-161.203) [-144.174] -- 0:03:47 14000 -- (-148.758) (-164.476) [-148.026] (-163.619) * (-167.210) [-142.411] (-159.641) (-150.062) -- 0:03:31 15000 -- [-143.019] (-167.681) (-149.851) (-158.037) * (-148.034) (-153.523) (-161.879) [-150.414] -- 0:03:17 Average standard deviation of split frequencies: 0.070331 16000 -- (-143.843) (-160.103) [-149.430] (-155.530) * (-162.177) (-150.950) (-168.043) [-145.360] -- 0:03:04 17000 -- (-148.658) (-157.367) [-143.684] (-164.197) * (-159.413) [-148.027] (-163.457) (-156.853) -- 0:02:53 18000 -- (-145.841) (-154.872) [-146.232] (-160.442) * [-149.032] (-148.909) (-159.338) (-150.977) -- 0:03:38 19000 -- [-144.705] (-159.390) (-148.701) (-163.863) * (-147.983) [-147.836] (-158.534) (-152.457) -- 0:03:26 20000 -- [-145.807] (-164.942) (-156.661) (-150.382) * (-147.430) [-143.493] (-164.345) (-158.665) -- 0:03:16 Average standard deviation of split frequencies: 0.062727 21000 -- (-142.196) (-165.449) [-147.554] (-148.813) * [-144.632] (-154.511) (-157.318) (-149.460) -- 0:03:06 22000 -- [-145.526] (-156.170) (-149.506) (-165.414) * [-143.338] (-155.336) (-160.242) (-149.814) -- 0:02:57 23000 -- (-152.367) (-161.592) [-150.258] (-161.795) * (-140.714) [-148.357] (-168.155) (-146.551) -- 0:03:32 24000 -- (-148.829) (-162.398) [-146.581] (-162.603) * (-149.160) [-149.194] (-162.969) (-149.142) -- 0:03:23 25000 -- (-148.580) (-158.518) [-150.189] (-164.977) * (-160.513) [-143.072] (-160.155) (-148.888) -- 0:03:15 Average standard deviation of split frequencies: 0.055993 26000 -- [-154.326] (-166.332) (-149.650) (-166.127) * [-143.873] (-148.215) (-164.241) (-146.098) -- 0:03:07 27000 -- (-149.465) [-147.237] (-146.651) (-165.449) * (-143.732) [-139.486] (-165.059) (-147.525) -- 0:03:00 28000 -- [-139.549] (-151.796) (-147.052) (-165.939) * (-148.669) (-150.257) (-170.066) [-144.715] -- 0:02:53 29000 -- (-138.461) (-157.086) [-142.897] (-161.524) * (-155.476) (-145.404) (-165.471) [-144.535] -- 0:03:20 30000 -- (-151.655) [-145.903] (-144.146) (-164.703) * (-150.235) [-148.909] (-159.456) (-145.988) -- 0:03:14 Average standard deviation of split frequencies: 0.044252 31000 -- (-161.564) [-142.025] (-147.502) (-161.938) * (-152.822) (-142.724) (-159.031) [-146.348] -- 0:03:07 32000 -- [-144.667] (-148.632) (-148.229) (-160.769) * (-155.389) (-146.364) (-166.021) [-142.686] -- 0:03:01 33000 -- (-154.983) (-145.541) [-146.326] (-168.230) * (-150.248) (-148.352) (-158.532) [-148.054] -- 0:02:55 34000 -- (-144.943) (-144.440) [-141.941] (-164.993) * (-142.601) (-150.275) (-160.517) [-149.155] -- 0:03:18 35000 -- (-148.047) (-161.366) [-139.705] (-168.384) * [-144.498] (-149.818) (-165.203) (-147.872) -- 0:03:13 Average standard deviation of split frequencies: 0.046042 36000 -- [-144.279] (-147.524) (-148.856) (-162.504) * [-149.708] (-146.332) (-166.491) (-150.691) -- 0:03:07 37000 -- (-144.630) (-146.733) [-142.189] (-155.375) * (-142.746) (-150.185) (-171.833) [-144.956] -- 0:03:02 38000 -- [-144.795] (-149.143) (-145.231) (-164.632) * (-147.808) (-149.387) (-174.463) [-144.145] -- 0:02:57 39000 -- (-145.449) (-146.589) [-140.497] (-156.345) * [-142.737] (-140.958) (-159.133) (-149.897) -- 0:03:17 40000 -- (-146.754) [-146.192] (-143.394) (-158.833) * [-142.106] (-144.658) (-161.160) (-150.406) -- 0:03:12 Average standard deviation of split frequencies: 0.042228 41000 -- (-151.028) (-154.805) [-144.157] (-157.057) * (-150.753) [-148.606] (-164.226) (-143.605) -- 0:03:07 42000 -- (-143.095) (-149.920) [-147.999] (-160.701) * (-143.353) [-142.425] (-164.371) (-143.338) -- 0:03:02 43000 -- [-145.497] (-145.500) (-146.754) (-165.730) * [-139.675] (-145.935) (-159.854) (-154.940) -- 0:02:58 44000 -- (-154.420) (-150.106) [-141.000] (-158.201) * (-146.748) [-145.583] (-166.920) (-144.388) -- 0:03:15 45000 -- (-144.264) (-148.924) [-147.812] (-164.045) * [-143.201] (-148.054) (-157.799) (-151.466) -- 0:03:11 Average standard deviation of split frequencies: 0.040309 46000 -- (-142.973) [-142.568] (-149.595) (-170.280) * [-139.633] (-159.965) (-164.217) (-156.448) -- 0:03:06 47000 -- [-146.018] (-146.146) (-147.467) (-168.871) * (-147.345) [-143.677] (-161.796) (-145.900) -- 0:03:02 48000 -- [-142.608] (-147.530) (-150.127) (-173.990) * (-142.009) (-154.066) (-163.445) [-151.226] -- 0:02:58 49000 -- (-142.904) (-149.050) [-145.090] (-163.588) * (-144.380) (-143.649) (-166.573) [-145.469] -- 0:02:54 50000 -- (-148.535) (-154.352) [-142.169] (-169.236) * [-148.319] (-142.428) (-158.896) (-151.886) -- 0:03:10 Average standard deviation of split frequencies: 0.035493 51000 -- (-148.747) [-143.655] (-140.649) (-165.859) * (-143.966) [-144.428] (-161.323) (-146.892) -- 0:03:06 52000 -- [-146.769] (-154.336) (-143.696) (-163.936) * (-144.975) [-143.350] (-162.340) (-164.337) -- 0:03:02 53000 -- [-148.056] (-146.113) (-146.962) (-155.071) * [-141.940] (-149.777) (-162.392) (-166.470) -- 0:02:58 54000 -- (-151.071) [-146.117] (-145.720) (-162.326) * [-145.413] (-144.259) (-159.705) (-170.260) -- 0:02:55 55000 -- [-149.136] (-151.415) (-146.715) (-165.807) * [-144.105] (-142.779) (-159.001) (-164.637) -- 0:03:09 Average standard deviation of split frequencies: 0.035938 56000 -- (-144.732) (-147.158) [-145.181] (-171.850) * (-147.421) [-151.653] (-163.932) (-163.999) -- 0:03:05 57000 -- (-148.280) [-146.372] (-145.806) (-154.035) * (-146.437) [-152.007] (-159.061) (-170.060) -- 0:03:01 58000 -- (-145.595) [-146.223] (-151.529) (-162.557) * [-145.610] (-151.563) (-151.269) (-165.917) -- 0:02:58 59000 -- (-150.672) (-142.539) [-147.189] (-161.605) * (-148.209) [-149.505] (-140.789) (-164.323) -- 0:02:55 60000 -- [-144.797] (-145.181) (-145.129) (-158.912) * [-148.756] (-151.235) (-144.603) (-158.701) -- 0:03:08 Average standard deviation of split frequencies: 0.030249 61000 -- [-140.160] (-154.267) (-147.727) (-153.941) * (-153.854) (-147.034) [-142.502] (-163.165) -- 0:03:04 62000 -- (-156.456) (-147.831) [-146.958] (-157.862) * (-150.419) (-141.467) [-145.276] (-159.907) -- 0:03:01 63000 -- (-142.445) (-149.300) [-140.108] (-166.971) * [-145.508] (-146.957) (-145.196) (-164.478) -- 0:02:58 64000 -- [-144.042] (-145.205) (-143.422) (-160.901) * [-148.597] (-146.661) (-147.598) (-158.251) -- 0:02:55 65000 -- (-144.983) [-149.787] (-145.871) (-155.776) * (-155.005) (-146.491) [-148.352] (-155.792) -- 0:03:07 Average standard deviation of split frequencies: 0.028324 66000 -- (-149.406) (-140.237) [-151.464] (-159.084) * [-145.194] (-151.434) (-150.539) (-155.884) -- 0:03:03 67000 -- [-147.514] (-154.365) (-150.357) (-164.634) * (-146.914) [-144.327] (-164.017) (-158.268) -- 0:03:01 68000 -- (-148.575) [-143.039] (-150.464) (-158.189) * (-145.855) (-149.272) [-149.984] (-164.806) -- 0:02:58 69000 -- [-149.637] (-156.236) (-146.738) (-156.021) * (-150.385) [-150.336] (-157.367) (-156.582) -- 0:02:55 70000 -- [-154.522] (-155.910) (-140.572) (-159.499) * [-151.716] (-139.403) (-150.629) (-157.695) -- 0:03:06 Average standard deviation of split frequencies: 0.027160 71000 -- (-152.396) (-154.397) [-148.863] (-166.199) * (-145.645) (-148.286) [-151.266] (-168.183) -- 0:03:03 72000 -- (-143.500) (-148.999) [-150.141] (-165.300) * (-162.992) [-144.637] (-144.519) (-157.733) -- 0:03:00 73000 -- (-163.445) (-151.482) [-147.259] (-162.215) * [-147.596] (-157.197) (-140.176) (-156.851) -- 0:02:57 74000 -- (-150.211) [-146.602] (-154.517) (-157.819) * (-156.917) [-148.765] (-146.564) (-160.010) -- 0:02:55 75000 -- (-145.043) (-145.686) [-151.073] (-163.147) * [-150.867] (-147.249) (-153.390) (-157.000) -- 0:02:52 Average standard deviation of split frequencies: 0.029302 76000 -- (-145.297) (-153.701) [-145.944] (-158.980) * (-145.914) [-144.729] (-152.535) (-159.266) -- 0:03:02 77000 -- [-145.862] (-159.225) (-147.811) (-157.361) * [-145.376] (-146.532) (-154.988) (-162.888) -- 0:02:59 78000 -- (-161.610) (-156.876) [-144.618] (-158.213) * (-148.893) [-144.236] (-160.047) (-156.413) -- 0:02:57 79000 -- [-146.608] (-163.781) (-143.704) (-156.909) * [-144.894] (-144.273) (-156.309) (-163.354) -- 0:02:54 80000 -- (-139.150) (-154.691) [-148.140] (-163.920) * [-143.750] (-146.290) (-159.110) (-161.133) -- 0:02:52 Average standard deviation of split frequencies: 0.030132 81000 -- (-153.061) (-169.836) [-146.593] (-159.483) * [-144.837] (-143.642) (-161.163) (-154.148) -- 0:03:01 82000 -- (-143.667) (-164.714) [-142.859] (-163.639) * [-142.404] (-150.796) (-169.283) (-164.289) -- 0:02:59 83000 -- (-149.673) (-160.437) [-144.084] (-167.766) * (-143.962) [-142.503] (-160.103) (-165.687) -- 0:02:56 84000 -- [-145.928] (-162.216) (-144.731) (-164.743) * (-148.756) [-147.756] (-163.013) (-164.672) -- 0:02:54 85000 -- [-147.371] (-154.717) (-148.449) (-154.852) * [-138.814] (-146.781) (-169.375) (-162.502) -- 0:02:52 Average standard deviation of split frequencies: 0.026078 86000 -- [-147.632] (-167.986) (-146.249) (-157.663) * (-143.869) [-147.000] (-161.446) (-161.976) -- 0:03:00 87000 -- (-148.600) (-152.522) [-140.551] (-157.077) * (-144.941) [-146.696] (-155.836) (-163.603) -- 0:02:58 88000 -- (-146.951) (-158.992) [-143.148] (-161.262) * [-142.787] (-149.262) (-159.824) (-158.912) -- 0:02:56 89000 -- [-147.755] (-156.260) (-146.617) (-159.026) * (-142.564) [-145.492] (-161.944) (-153.857) -- 0:02:54 90000 -- [-146.108] (-157.969) (-149.894) (-158.303) * [-143.024] (-147.887) (-157.576) (-151.441) -- 0:02:51 Average standard deviation of split frequencies: 0.024823 91000 -- (-143.488) (-159.213) [-141.255] (-152.207) * [-142.766] (-152.296) (-169.464) (-153.723) -- 0:02:59 92000 -- (-142.598) (-161.743) [-141.149] (-162.869) * (-154.709) [-144.676] (-157.492) (-159.260) -- 0:02:57 93000 -- (-143.031) (-156.832) [-140.333] (-157.162) * [-142.111] (-144.613) (-164.816) (-154.147) -- 0:02:55 94000 -- [-141.470] (-163.092) (-141.464) (-157.551) * (-147.828) [-145.750] (-166.686) (-162.240) -- 0:02:53 95000 -- (-151.671) (-157.710) [-144.451] (-154.565) * (-144.564) [-145.898] (-155.667) (-164.085) -- 0:02:51 Average standard deviation of split frequencies: 0.023407 96000 -- (-151.168) (-156.792) [-142.247] (-147.335) * (-148.484) [-149.170] (-162.017) (-163.089) -- 0:02:58 97000 -- [-139.764] (-157.676) (-144.500) (-152.196) * (-153.076) (-144.641) [-142.008] (-166.922) -- 0:02:56 98000 -- (-145.985) (-161.498) (-150.869) [-142.656] * [-140.261] (-158.647) (-150.304) (-156.464) -- 0:02:54 99000 -- (-144.244) (-158.429) (-145.360) [-144.176] * [-143.445] (-148.323) (-151.232) (-160.669) -- 0:02:52 100000 -- (-153.132) (-153.233) [-139.849] (-157.509) * (-153.511) (-143.817) [-142.215] (-156.439) -- 0:02:51 Average standard deviation of split frequencies: 0.027595 101000 -- (-149.638) (-154.452) [-138.492] (-148.621) * (-157.242) [-147.300] (-141.332) (-152.680) -- 0:02:58 102000 -- (-147.255) (-166.243) (-140.085) [-148.375] * (-159.610) (-147.161) [-152.259] (-156.844) -- 0:02:56 103000 -- (-154.098) (-152.857) [-148.767] (-148.170) * (-153.573) (-145.887) [-141.086] (-149.241) -- 0:02:54 104000 -- [-138.249] (-159.894) (-149.111) (-151.730) * (-163.163) [-140.953] (-143.332) (-155.883) -- 0:02:52 105000 -- (-143.905) (-156.526) [-148.594] (-159.913) * (-155.904) [-143.912] (-146.606) (-159.335) -- 0:02:50 Average standard deviation of split frequencies: 0.026832 106000 -- (-146.369) (-161.757) [-148.444] (-156.202) * (-161.310) (-145.443) [-143.303] (-155.229) -- 0:02:48 107000 -- [-145.988] (-160.237) (-148.084) (-163.467) * (-159.120) (-144.608) [-147.893] (-156.799) -- 0:02:55 108000 -- [-140.302] (-155.816) (-141.585) (-161.090) * (-161.657) (-148.937) [-148.449] (-171.633) -- 0:02:53 109000 -- (-144.071) (-162.791) [-143.870] (-161.797) * (-155.772) (-141.597) [-144.826] (-164.323) -- 0:02:51 110000 -- [-143.011] (-158.260) (-146.584) (-170.479) * (-161.518) [-146.747] (-149.747) (-165.003) -- 0:02:49 Average standard deviation of split frequencies: 0.028643 111000 -- (-142.253) (-152.038) [-149.773] (-160.633) * (-158.160) (-146.786) [-145.120] (-160.256) -- 0:02:48 112000 -- [-144.985] (-158.122) (-147.965) (-158.130) * (-165.134) [-145.572] (-150.953) (-165.582) -- 0:02:54 113000 -- (-144.094) (-153.807) [-147.000] (-158.596) * (-161.594) (-144.758) [-147.770] (-166.232) -- 0:02:52 114000 -- (-143.776) (-164.463) [-147.330] (-152.690) * (-162.777) (-148.129) [-144.044] (-151.839) -- 0:02:50 115000 -- [-146.652] (-158.914) (-155.070) (-158.601) * (-167.726) [-139.992] (-156.257) (-151.710) -- 0:02:49 Average standard deviation of split frequencies: 0.028167 116000 -- [-152.095] (-171.287) (-147.282) (-164.051) * (-158.929) [-147.308] (-148.418) (-149.864) -- 0:02:47 117000 -- [-148.513] (-170.040) (-149.877) (-159.845) * (-159.651) [-144.836] (-144.684) (-155.482) -- 0:02:53 118000 -- [-148.162] (-160.876) (-149.207) (-159.454) * (-160.522) (-147.059) [-143.302] (-152.470) -- 0:02:51 119000 -- [-144.501] (-164.661) (-149.464) (-163.064) * (-161.456) (-143.718) [-149.506] (-154.703) -- 0:02:50 120000 -- [-150.773] (-167.208) (-147.531) (-162.317) * (-162.326) [-142.266] (-150.001) (-156.396) -- 0:02:48 Average standard deviation of split frequencies: 0.026404 121000 -- [-146.392] (-166.797) (-153.216) (-166.314) * (-160.265) [-141.764] (-145.880) (-161.857) -- 0:02:47 122000 -- (-152.610) (-159.911) [-147.729] (-164.923) * (-154.238) [-142.859] (-146.001) (-166.419) -- 0:02:52 123000 -- (-142.137) (-149.336) [-141.469] (-163.615) * (-151.696) (-147.665) [-145.220] (-157.279) -- 0:02:51 124000 -- (-154.800) [-151.522] (-143.609) (-165.023) * (-152.349) (-151.026) [-144.408] (-165.932) -- 0:02:49 125000 -- (-157.555) [-140.078] (-145.908) (-160.847) * (-158.711) (-152.156) [-140.888] (-152.911) -- 0:02:48 Average standard deviation of split frequencies: 0.025802 126000 -- (-145.393) (-147.254) [-141.156] (-165.284) * (-160.031) (-147.261) [-143.893] (-160.848) -- 0:02:46 127000 -- (-151.901) (-155.269) [-145.890] (-156.325) * (-155.177) (-144.512) [-148.396] (-152.089) -- 0:02:44 128000 -- (-142.907) [-140.535] (-153.917) (-163.528) * (-165.039) (-146.580) (-146.575) [-146.325] -- 0:02:50 129000 -- [-149.453] (-153.250) (-157.660) (-160.797) * (-156.888) (-151.402) [-147.002] (-146.888) -- 0:02:48 130000 -- [-146.962] (-150.676) (-156.132) (-164.058) * (-157.828) (-152.706) [-150.637] (-143.422) -- 0:02:47 Average standard deviation of split frequencies: 0.023089 131000 -- (-148.350) [-144.850] (-161.316) (-162.928) * (-160.516) (-140.185) (-157.365) [-147.658] -- 0:02:45 132000 -- [-145.150] (-153.060) (-167.183) (-164.579) * (-159.790) [-144.190] (-157.642) (-151.723) -- 0:02:44 133000 -- [-142.315] (-149.886) (-166.359) (-158.798) * (-164.482) [-146.369] (-165.823) (-144.390) -- 0:02:49 134000 -- (-147.687) [-141.400] (-162.504) (-157.002) * (-155.178) (-139.594) (-164.952) [-143.304] -- 0:02:48 135000 -- (-144.704) [-143.525] (-157.770) (-167.328) * (-157.211) [-143.802] (-161.950) (-152.206) -- 0:02:46 Average standard deviation of split frequencies: 0.022829 136000 -- [-140.023] (-147.416) (-163.062) (-157.271) * (-157.151) [-150.645] (-166.502) (-147.499) -- 0:02:45 137000 -- [-142.811] (-145.158) (-157.580) (-163.745) * (-161.852) [-144.364] (-163.185) (-145.106) -- 0:02:43 138000 -- (-151.321) [-148.061] (-146.748) (-168.600) * (-155.734) (-144.802) (-157.180) [-146.044] -- 0:02:48 139000 -- (-145.951) [-150.408] (-143.420) (-152.068) * (-152.904) (-144.006) (-159.287) [-143.263] -- 0:02:47 140000 -- [-138.111] (-143.605) (-151.629) (-153.768) * (-162.364) [-147.588] (-159.720) (-156.649) -- 0:02:45 Average standard deviation of split frequencies: 0.023227 141000 -- (-143.863) [-145.410] (-144.452) (-166.794) * (-156.774) (-142.414) (-163.349) [-144.900] -- 0:02:44 142000 -- [-140.743] (-140.898) (-155.071) (-159.061) * (-161.970) (-148.252) (-159.971) [-140.208] -- 0:02:43 143000 -- [-140.248] (-143.696) (-141.492) (-156.934) * (-154.867) [-144.148] (-151.829) (-149.413) -- 0:02:47 144000 -- (-153.077) [-143.876] (-150.574) (-153.007) * (-156.364) [-148.199] (-162.194) (-150.203) -- 0:02:46 145000 -- [-144.493] (-149.203) (-146.864) (-151.661) * (-164.305) (-142.719) (-159.760) [-145.619] -- 0:02:45 Average standard deviation of split frequencies: 0.023785 146000 -- (-144.876) [-140.964] (-147.293) (-156.635) * (-158.037) (-152.312) (-154.648) [-143.821] -- 0:02:43 147000 -- (-155.348) (-149.987) [-139.744] (-161.256) * (-158.572) (-148.384) (-160.550) [-155.763] -- 0:02:42 148000 -- [-144.044] (-145.457) (-146.681) (-156.246) * (-165.520) [-147.514] (-159.412) (-153.817) -- 0:02:46 149000 -- (-152.885) [-146.752] (-148.004) (-154.454) * (-164.958) [-143.733] (-167.125) (-143.988) -- 0:02:45 150000 -- (-156.811) (-142.624) [-144.352] (-165.374) * (-153.874) (-144.340) (-156.376) [-142.587] -- 0:02:44 Average standard deviation of split frequencies: 0.023088 151000 -- (-150.142) [-138.525] (-149.170) (-157.443) * (-163.454) (-144.391) (-157.598) [-144.749] -- 0:02:43 152000 -- (-152.244) (-141.639) [-145.191] (-156.959) * (-165.346) [-144.970] (-160.342) (-149.834) -- 0:02:41 153000 -- (-145.938) (-144.464) [-141.804] (-161.653) * (-157.262) (-147.050) (-158.849) [-146.193] -- 0:02:40 154000 -- (-151.968) (-149.321) [-141.662] (-163.576) * (-155.221) (-147.469) (-158.618) [-149.126] -- 0:02:44 155000 -- [-153.560] (-144.294) (-146.332) (-160.643) * (-163.327) [-142.155] (-151.391) (-145.003) -- 0:02:43 Average standard deviation of split frequencies: 0.023095 156000 -- (-144.552) (-148.234) [-145.325] (-157.499) * (-160.078) [-148.281] (-147.897) (-152.956) -- 0:02:42 157000 -- (-154.966) [-147.526] (-147.522) (-153.245) * (-162.102) [-147.865] (-149.392) (-150.649) -- 0:02:41 158000 -- (-141.195) (-141.633) [-142.338] (-166.345) * (-159.136) (-158.790) (-154.466) [-148.542] -- 0:02:39 159000 -- (-151.068) (-143.557) [-145.547] (-159.668) * (-163.758) [-142.060] (-154.014) (-153.391) -- 0:02:43 160000 -- (-151.234) (-144.999) [-144.634] (-166.562) * (-156.588) (-149.123) [-147.523] (-146.466) -- 0:02:42 Average standard deviation of split frequencies: 0.024416 161000 -- (-151.297) [-142.516] (-146.773) (-169.331) * (-157.634) [-142.286] (-146.751) (-149.272) -- 0:02:41 162000 -- (-143.995) (-144.546) [-146.452] (-170.694) * (-159.731) [-142.635] (-144.455) (-150.056) -- 0:02:40 163000 -- (-161.480) [-151.694] (-151.580) (-157.673) * (-162.372) (-151.325) (-147.291) [-142.895] -- 0:02:39 164000 -- (-150.148) (-152.034) [-151.378] (-163.309) * (-169.465) (-150.091) [-150.294] (-151.938) -- 0:02:43 165000 -- (-147.799) (-151.431) [-148.981] (-158.869) * (-162.604) [-146.160] (-149.432) (-146.098) -- 0:02:41 Average standard deviation of split frequencies: 0.023110 166000 -- [-140.846] (-155.740) (-148.215) (-161.889) * (-166.117) [-144.244] (-152.098) (-145.904) -- 0:02:40 167000 -- (-146.226) [-153.725] (-143.965) (-163.483) * (-156.427) (-167.387) (-148.852) [-144.108] -- 0:02:39 168000 -- (-152.370) (-147.206) [-149.191] (-158.985) * (-161.240) (-145.180) (-145.629) [-146.217] -- 0:02:38 169000 -- [-148.997] (-153.438) (-142.415) (-162.081) * (-163.157) (-148.448) (-147.776) [-144.504] -- 0:02:37 170000 -- (-144.596) (-152.013) [-142.779] (-159.705) * (-160.002) (-161.153) [-139.210] (-143.254) -- 0:02:41 Average standard deviation of split frequencies: 0.022886 171000 -- [-145.495] (-147.964) (-146.640) (-159.878) * (-165.388) [-143.756] (-150.285) (-150.740) -- 0:02:39 172000 -- (-166.142) (-148.638) [-140.022] (-167.653) * (-162.329) [-146.526] (-145.856) (-153.714) -- 0:02:38 173000 -- (-160.947) (-151.274) [-145.363] (-157.789) * (-163.310) (-148.925) (-145.631) [-152.400] -- 0:02:37 174000 -- (-160.965) (-152.255) [-142.705] (-163.197) * (-159.888) (-144.581) (-145.914) [-144.832] -- 0:02:36 175000 -- (-166.034) (-145.178) [-150.899] (-158.186) * (-163.634) (-161.400) [-145.399] (-147.975) -- 0:02:40 Average standard deviation of split frequencies: 0.022480 176000 -- (-162.332) (-155.696) [-142.798] (-155.484) * (-160.503) (-150.587) [-139.585] (-149.957) -- 0:02:39 177000 -- (-162.816) [-143.026] (-147.499) (-162.000) * (-153.802) (-159.938) [-139.494] (-147.250) -- 0:02:38 178000 -- (-158.273) [-145.871] (-151.356) (-165.979) * (-159.157) (-148.542) (-146.609) [-144.778] -- 0:02:37 179000 -- (-159.970) (-138.732) [-145.192] (-158.792) * (-153.749) [-140.013] (-147.961) (-147.850) -- 0:02:35 180000 -- (-163.853) (-147.667) [-149.329] (-166.565) * (-157.673) [-139.865] (-147.306) (-142.413) -- 0:02:39 Average standard deviation of split frequencies: 0.020584 181000 -- (-155.320) (-149.410) [-146.207] (-160.148) * (-153.081) (-144.988) (-142.899) [-145.906] -- 0:02:38 182000 -- [-141.520] (-141.146) (-145.427) (-170.783) * (-152.886) [-146.623] (-144.744) (-153.570) -- 0:02:37 183000 -- (-145.854) (-147.275) [-146.327] (-169.004) * (-154.029) [-142.075] (-150.693) (-143.128) -- 0:02:36 184000 -- (-146.016) [-147.104] (-149.594) (-163.109) * (-160.599) (-148.585) [-148.283] (-144.155) -- 0:02:35 185000 -- (-149.025) [-152.219] (-147.660) (-156.881) * (-156.313) [-144.039] (-146.432) (-143.903) -- 0:02:38 Average standard deviation of split frequencies: 0.019642 186000 -- (-142.440) (-149.309) [-147.634] (-163.007) * (-161.675) [-148.025] (-147.644) (-156.403) -- 0:02:37 187000 -- [-144.907] (-151.484) (-148.732) (-156.920) * (-151.099) (-161.961) (-154.062) [-149.272] -- 0:02:36 188000 -- (-148.510) (-141.712) [-143.921] (-162.197) * (-158.170) (-150.353) [-146.538] (-158.009) -- 0:02:35 189000 -- (-145.993) [-146.534] (-154.010) (-155.580) * (-163.642) [-154.082] (-151.922) (-153.488) -- 0:02:34 190000 -- (-144.614) [-149.504] (-145.669) (-150.509) * (-157.179) (-158.005) (-145.302) [-143.286] -- 0:02:37 Average standard deviation of split frequencies: 0.020662 191000 -- (-155.662) (-150.199) [-144.118] (-157.618) * (-155.507) [-152.946] (-146.438) (-145.873) -- 0:02:36 192000 -- (-157.812) (-152.950) [-148.530] (-155.136) * (-163.924) (-155.114) [-146.573] (-151.900) -- 0:02:35 193000 -- (-164.379) [-148.652] (-154.858) (-161.341) * (-158.545) (-150.120) (-145.971) [-143.991] -- 0:02:34 194000 -- (-157.736) (-151.068) [-151.701] (-165.617) * (-156.725) [-140.595] (-149.432) (-145.994) -- 0:02:33 195000 -- (-170.317) [-145.877] (-158.422) (-158.639) * (-160.949) (-147.905) (-143.739) [-147.292] -- 0:02:32 Average standard deviation of split frequencies: 0.020568 196000 -- (-156.086) [-145.575] (-158.342) (-161.965) * (-165.239) (-152.406) [-155.595] (-152.003) -- 0:02:35 197000 -- (-163.026) [-142.265] (-147.431) (-156.600) * (-164.635) [-144.116] (-155.122) (-156.873) -- 0:02:34 198000 -- (-155.985) (-154.718) [-144.880] (-172.969) * (-160.647) [-150.781] (-148.663) (-148.428) -- 0:02:33 199000 -- (-148.319) (-148.027) [-148.681] (-164.145) * (-162.372) (-149.757) [-145.854] (-144.375) -- 0:02:32 200000 -- (-153.545) (-144.921) [-151.693] (-168.788) * (-161.622) [-149.418] (-146.410) (-140.570) -- 0:02:32 Average standard deviation of split frequencies: 0.022066 201000 -- (-162.172) [-143.604] (-148.616) (-164.610) * (-164.893) (-151.214) (-143.345) [-149.728] -- 0:02:35 202000 -- (-163.719) [-140.619] (-146.665) (-166.346) * (-152.242) (-151.630) [-142.476] (-145.327) -- 0:02:34 203000 -- (-160.835) [-144.472] (-148.380) (-164.007) * (-156.764) (-153.127) (-147.623) [-144.087] -- 0:02:33 204000 -- (-161.266) (-154.752) [-147.979] (-153.041) * (-158.187) (-150.036) [-143.488] (-146.740) -- 0:02:32 205000 -- (-162.352) [-148.942] (-145.311) (-163.061) * (-157.902) (-149.732) (-152.465) [-139.305] -- 0:02:31 Average standard deviation of split frequencies: 0.022174 206000 -- (-158.796) [-145.703] (-147.181) (-161.986) * (-155.291) (-148.403) (-150.772) [-140.453] -- 0:02:34 207000 -- (-157.238) [-149.968] (-146.497) (-171.264) * (-154.810) (-147.608) (-156.844) [-145.221] -- 0:02:33 208000 -- (-152.908) (-149.013) [-144.894] (-163.828) * (-165.551) [-145.352] (-156.901) (-141.841) -- 0:02:32 209000 -- (-156.501) [-147.542] (-146.434) (-170.484) * (-153.868) [-146.273] (-160.026) (-147.728) -- 0:02:31 210000 -- (-164.457) (-149.604) [-145.048] (-167.904) * (-154.408) [-148.501] (-151.526) (-143.736) -- 0:02:30 Average standard deviation of split frequencies: 0.020219 211000 -- (-153.695) [-147.049] (-152.553) (-164.115) * (-158.870) (-169.603) (-145.082) [-146.727] -- 0:02:33 212000 -- [-145.772] (-146.280) (-141.532) (-158.019) * (-158.128) (-155.561) [-150.815] (-155.227) -- 0:02:32 213000 -- (-144.286) (-153.439) [-139.894] (-162.614) * (-162.784) (-157.537) (-139.994) [-144.302] -- 0:02:31 214000 -- (-143.999) (-146.044) [-140.859] (-158.353) * (-164.280) (-165.641) (-148.997) [-144.556] -- 0:02:30 215000 -- [-145.065] (-153.073) (-148.299) (-156.949) * (-170.328) (-156.696) [-145.793] (-142.173) -- 0:02:29 Average standard deviation of split frequencies: 0.018137 216000 -- [-141.890] (-145.199) (-142.389) (-167.863) * (-157.066) (-160.800) [-145.967] (-147.764) -- 0:02:28 217000 -- (-159.438) [-154.280] (-146.390) (-163.219) * (-163.435) (-163.966) (-144.892) [-147.298] -- 0:02:31 218000 -- (-157.226) (-147.163) [-148.510] (-169.290) * (-159.743) (-159.626) (-146.350) [-146.367] -- 0:02:30 219000 -- (-161.685) (-149.542) [-146.745] (-162.816) * (-158.400) (-162.138) (-145.810) [-144.128] -- 0:02:29 220000 -- (-160.669) (-144.712) [-146.140] (-159.752) * (-155.041) (-163.607) (-147.166) [-149.780] -- 0:02:28 Average standard deviation of split frequencies: 0.018158 221000 -- (-157.004) (-143.149) [-148.488] (-153.612) * (-160.448) (-161.256) (-147.552) [-140.933] -- 0:02:28 222000 -- (-164.934) (-143.212) [-146.516] (-154.634) * (-165.816) (-152.293) (-148.766) [-145.612] -- 0:02:30 223000 -- (-158.221) (-142.315) [-144.674] (-154.598) * (-152.906) (-157.439) (-152.897) [-143.398] -- 0:02:29 224000 -- (-171.643) (-145.112) [-141.295] (-160.699) * (-156.947) (-158.334) (-144.671) [-145.089] -- 0:02:28 225000 -- (-168.808) [-146.699] (-156.483) (-161.869) * (-157.228) (-159.280) (-145.975) [-144.813] -- 0:02:28 Average standard deviation of split frequencies: 0.017357 226000 -- (-169.137) [-145.334] (-147.552) (-165.982) * (-153.893) (-153.313) [-146.711] (-141.999) -- 0:02:27 227000 -- (-154.301) (-142.710) [-147.995] (-154.610) * (-160.731) (-160.869) [-152.854] (-145.387) -- 0:02:29 228000 -- (-164.481) (-149.431) [-151.357] (-160.936) * (-155.238) (-159.738) (-150.173) [-147.061] -- 0:02:28 229000 -- (-160.422) [-147.175] (-140.237) (-165.232) * (-156.549) (-165.504) (-147.714) [-142.762] -- 0:02:28 230000 -- (-155.461) (-151.965) [-141.792] (-158.391) * (-165.532) (-161.725) (-144.018) [-145.596] -- 0:02:27 Average standard deviation of split frequencies: 0.018612 231000 -- (-158.530) [-149.190] (-143.367) (-159.717) * (-169.123) (-164.729) [-143.531] (-150.531) -- 0:02:26 232000 -- (-162.936) [-142.565] (-147.164) (-168.731) * (-155.038) (-154.883) (-146.614) [-145.684] -- 0:02:28 233000 -- (-162.200) [-145.385] (-143.929) (-149.234) * (-157.396) (-157.762) [-145.331] (-150.529) -- 0:02:28 234000 -- (-167.220) [-144.420] (-151.603) (-142.096) * (-155.546) (-155.727) [-145.979] (-150.603) -- 0:02:27 235000 -- (-159.311) (-145.452) (-152.637) [-152.130] * (-157.444) (-163.339) [-144.987] (-146.849) -- 0:02:26 Average standard deviation of split frequencies: 0.018191 236000 -- (-170.941) (-141.066) (-144.935) [-143.312] * (-158.345) (-158.371) (-150.832) [-144.410] -- 0:02:25 237000 -- (-162.043) (-145.110) [-146.517] (-163.780) * (-161.001) (-162.666) [-146.560] (-151.080) -- 0:02:28 238000 -- (-157.081) [-146.752] (-149.120) (-154.081) * (-157.970) (-166.846) (-150.939) [-147.567] -- 0:02:27 239000 -- (-162.113) (-147.555) [-142.662] (-161.746) * (-157.098) (-165.140) [-146.302] (-152.903) -- 0:02:26 240000 -- (-164.290) (-156.552) [-141.565] (-156.048) * (-159.324) (-155.372) (-143.766) [-146.137] -- 0:02:25 Average standard deviation of split frequencies: 0.018258 241000 -- (-169.435) [-145.246] (-142.682) (-162.717) * (-159.921) (-155.929) [-147.907] (-154.589) -- 0:02:24 242000 -- (-160.905) [-148.854] (-145.721) (-155.899) * (-159.834) (-158.476) (-155.968) [-149.436] -- 0:02:24 243000 -- (-156.381) (-144.633) [-141.211] (-159.986) * (-160.003) (-160.926) [-148.116] (-145.280) -- 0:02:26 244000 -- (-157.185) [-146.484] (-148.231) (-165.210) * (-157.254) (-159.449) (-155.225) [-148.477] -- 0:02:25 245000 -- (-163.892) [-144.774] (-147.334) (-157.651) * (-158.414) (-170.449) (-147.490) [-142.120] -- 0:02:24 Average standard deviation of split frequencies: 0.018205 246000 -- (-158.154) (-142.784) [-148.204] (-163.209) * (-161.591) (-158.559) [-149.242] (-153.494) -- 0:02:24 247000 -- (-162.678) [-140.168] (-159.962) (-152.179) * (-167.004) (-160.634) (-145.641) [-145.564] -- 0:02:23 248000 -- (-160.356) [-147.625] (-143.294) (-154.389) * (-160.486) (-161.009) (-150.683) [-148.136] -- 0:02:25 249000 -- (-162.642) (-144.480) [-144.176] (-145.990) * (-157.743) (-170.561) (-151.127) [-144.479] -- 0:02:24 250000 -- (-162.901) (-147.242) (-144.856) [-149.901] * (-164.527) (-165.251) [-154.967] (-146.866) -- 0:02:24 Average standard deviation of split frequencies: 0.017622 251000 -- (-163.046) [-153.910] (-162.516) (-144.041) * (-162.593) (-163.057) (-139.776) [-153.080] -- 0:02:23 252000 -- (-165.810) [-139.094] (-158.479) (-148.207) * (-161.018) (-156.715) [-146.779] (-146.128) -- 0:02:22 253000 -- (-162.788) [-140.339] (-160.030) (-144.362) * (-163.667) (-165.410) [-149.611] (-144.094) -- 0:02:24 254000 -- (-162.238) [-145.441] (-164.741) (-148.454) * (-169.318) (-168.291) (-151.748) [-145.943] -- 0:02:23 255000 -- (-157.310) [-146.755] (-158.512) (-144.021) * (-163.541) (-164.101) (-145.860) [-143.774] -- 0:02:23 Average standard deviation of split frequencies: 0.017050 256000 -- (-170.759) [-150.385] (-154.155) (-144.913) * (-167.181) (-162.419) (-141.147) [-141.252] -- 0:02:22 257000 -- (-160.678) (-151.112) (-153.183) [-142.013] * (-157.391) (-160.083) (-140.995) [-141.175] -- 0:02:21 258000 -- (-160.594) (-152.713) [-146.700] (-141.819) * (-155.137) (-159.419) (-148.098) [-147.095] -- 0:02:23 259000 -- (-161.398) (-150.369) (-143.634) [-143.980] * (-157.881) (-163.413) (-145.186) [-148.632] -- 0:02:23 260000 -- (-162.421) (-148.409) [-142.847] (-151.034) * (-160.782) (-154.232) [-147.799] (-153.634) -- 0:02:22 Average standard deviation of split frequencies: 0.017549 261000 -- (-156.714) (-150.230) [-148.182] (-152.814) * (-156.074) (-162.472) (-147.137) [-141.755] -- 0:02:21 262000 -- (-157.356) [-148.360] (-150.029) (-158.942) * (-159.556) (-168.661) (-161.518) [-149.904] -- 0:02:20 263000 -- (-154.093) (-151.969) [-151.145] (-153.102) * (-162.313) (-158.770) [-143.559] (-150.653) -- 0:02:22 264000 -- (-163.077) (-156.132) [-144.200] (-149.360) * (-157.222) (-157.092) (-143.590) [-152.223] -- 0:02:22 265000 -- (-153.793) [-150.637] (-149.098) (-154.956) * (-159.897) (-143.228) [-148.257] (-155.833) -- 0:02:21 Average standard deviation of split frequencies: 0.016737 266000 -- (-154.356) (-151.634) [-147.931] (-159.423) * (-163.571) [-144.630] (-144.753) (-158.978) -- 0:02:20 267000 -- (-159.962) (-153.728) [-147.300] (-154.190) * (-163.225) [-145.041] (-145.078) (-161.549) -- 0:02:20 268000 -- (-153.567) [-147.822] (-147.803) (-154.579) * (-161.285) [-145.967] (-151.231) (-167.175) -- 0:02:19 269000 -- (-157.881) (-150.011) [-147.157] (-155.269) * (-165.249) (-145.221) [-148.887] (-160.169) -- 0:02:21 270000 -- (-164.029) (-150.873) (-148.658) [-147.922] * (-158.428) (-139.792) [-142.758] (-160.537) -- 0:02:20 Average standard deviation of split frequencies: 0.017158 271000 -- (-162.854) (-147.356) (-144.868) [-147.515] * (-162.227) (-150.279) [-147.094] (-155.354) -- 0:02:19 272000 -- (-164.499) (-154.657) [-143.455] (-158.406) * (-162.225) (-146.197) [-147.050] (-167.168) -- 0:02:19 273000 -- (-158.761) [-149.085] (-140.825) (-148.929) * (-162.839) [-143.088] (-144.037) (-160.963) -- 0:02:18 274000 -- (-159.292) (-144.067) (-151.659) [-145.743] * (-162.294) (-147.643) [-147.221] (-156.090) -- 0:02:20 275000 -- (-156.324) (-145.435) (-157.280) [-149.998] * (-163.802) (-145.660) [-145.084] (-159.214) -- 0:02:19 Average standard deviation of split frequencies: 0.016258 276000 -- (-164.064) (-150.670) (-143.737) [-144.633] * (-161.134) [-149.231] (-151.842) (-159.450) -- 0:02:19 277000 -- (-156.231) (-148.217) [-147.554] (-149.823) * (-159.072) (-149.768) [-144.399] (-163.098) -- 0:02:18 278000 -- (-158.244) [-142.561] (-142.353) (-144.245) * (-163.921) [-148.938] (-145.981) (-158.590) -- 0:02:17 279000 -- (-160.512) [-148.174] (-147.108) (-141.075) * (-171.573) (-144.412) [-144.368] (-155.437) -- 0:02:19 280000 -- (-159.753) (-148.399) (-150.470) [-144.361] * (-164.238) (-154.520) [-143.240] (-155.475) -- 0:02:18 Average standard deviation of split frequencies: 0.017119 281000 -- (-152.474) (-149.219) (-153.781) [-147.208] * (-158.456) (-144.756) [-147.270] (-149.555) -- 0:02:18 282000 -- (-160.031) [-148.532] (-147.911) (-152.598) * (-158.968) (-147.722) (-145.577) [-142.844] -- 0:02:17 283000 -- (-157.976) (-150.578) [-140.295] (-147.572) * (-167.556) (-152.147) [-148.829] (-152.419) -- 0:02:16 284000 -- (-155.736) (-151.883) [-145.823] (-140.472) * (-154.894) (-149.712) (-150.471) [-144.794] -- 0:02:18 285000 -- (-164.936) (-149.477) [-139.923] (-149.821) * (-163.307) (-152.079) (-148.705) [-137.743] -- 0:02:17 Average standard deviation of split frequencies: 0.016544 286000 -- (-155.765) (-160.478) [-142.851] (-162.656) * (-165.537) (-149.341) [-141.784] (-146.532) -- 0:02:17 287000 -- (-156.292) [-153.585] (-143.368) (-162.630) * (-170.845) (-152.024) [-144.612] (-153.713) -- 0:02:16 288000 -- (-151.335) (-145.257) (-145.498) [-152.084] * (-166.201) [-145.562] (-144.036) (-156.604) -- 0:02:15 289000 -- (-156.147) (-145.258) [-145.891] (-150.329) * (-169.587) (-149.516) [-143.501] (-164.069) -- 0:02:15 290000 -- (-167.508) (-150.452) [-144.737] (-148.169) * (-149.047) (-147.090) [-144.323] (-163.737) -- 0:02:17 Average standard deviation of split frequencies: 0.015871 291000 -- (-158.511) [-144.865] (-151.207) (-151.260) * (-147.194) [-150.997] (-151.378) (-158.493) -- 0:02:16 292000 -- (-161.515) (-150.683) (-148.739) [-145.583] * [-142.442] (-145.632) (-157.251) (-156.941) -- 0:02:15 293000 -- (-161.665) [-147.082] (-149.874) (-147.497) * [-145.529] (-144.832) (-158.915) (-161.316) -- 0:02:15 294000 -- (-169.018) [-144.003] (-147.089) (-156.946) * (-144.363) [-148.168] (-156.816) (-164.467) -- 0:02:14 295000 -- (-159.513) (-149.576) (-143.472) [-142.184] * (-145.519) [-145.903] (-149.959) (-156.174) -- 0:02:16 Average standard deviation of split frequencies: 0.016153 296000 -- (-162.690) [-139.320] (-147.413) (-148.099) * (-151.260) (-144.512) [-149.707] (-157.820) -- 0:02:15 297000 -- (-168.450) (-147.781) [-143.711] (-143.294) * (-155.515) [-147.736] (-151.468) (-160.078) -- 0:02:14 298000 -- (-155.091) (-151.542) [-144.198] (-153.224) * (-156.788) (-141.751) [-149.808] (-167.303) -- 0:02:14 299000 -- (-160.449) [-147.584] (-153.332) (-146.704) * [-139.991] (-152.599) (-144.172) (-159.606) -- 0:02:13 300000 -- (-154.550) [-145.845] (-172.070) (-144.306) * (-149.546) [-144.941] (-148.321) (-160.344) -- 0:02:15 Average standard deviation of split frequencies: 0.016375 301000 -- (-157.871) (-141.321) (-170.807) [-150.653] * (-142.775) (-154.550) [-150.642] (-158.516) -- 0:02:14 302000 -- [-143.398] (-154.205) (-169.982) (-151.088) * (-147.299) (-147.609) [-152.533] (-159.423) -- 0:02:14 303000 -- (-143.864) [-147.248] (-156.742) (-146.850) * [-142.437] (-153.421) (-139.748) (-152.904) -- 0:02:13 304000 -- (-151.674) (-146.958) (-170.880) [-147.765] * [-147.262] (-159.073) (-143.380) (-157.236) -- 0:02:12 305000 -- [-146.389] (-147.726) (-170.441) (-146.561) * [-153.601] (-158.319) (-141.344) (-154.879) -- 0:02:14 Average standard deviation of split frequencies: 0.016946 306000 -- (-144.347) (-151.541) (-174.937) [-143.781] * [-144.524] (-156.033) (-150.281) (-151.729) -- 0:02:13 307000 -- [-145.501] (-145.590) (-172.762) (-151.657) * [-141.770] (-160.505) (-147.038) (-156.519) -- 0:02:13 308000 -- (-144.463) (-148.368) (-173.179) [-142.925] * (-146.641) (-159.310) [-149.666] (-160.408) -- 0:02:12 309000 -- (-151.635) (-143.340) (-159.678) [-142.962] * [-142.172] (-145.661) (-150.746) (-163.436) -- 0:02:11 310000 -- (-147.876) [-141.016] (-162.527) (-146.288) * [-144.089] (-162.374) (-153.208) (-165.434) -- 0:02:13 Average standard deviation of split frequencies: 0.016925 311000 -- (-145.039) (-158.637) (-154.973) [-142.283] * (-143.205) (-160.428) [-148.853] (-159.777) -- 0:02:12 312000 -- (-145.903) (-160.759) (-159.435) [-139.135] * (-151.231) [-147.875] (-150.796) (-165.918) -- 0:02:12 313000 -- (-149.596) (-161.745) (-167.302) [-141.609] * (-144.284) (-158.694) [-147.185] (-166.427) -- 0:02:11 314000 -- [-146.816] (-156.367) (-166.945) (-146.486) * [-151.396] (-167.036) (-150.491) (-167.691) -- 0:02:11 315000 -- [-147.762] (-159.195) (-167.557) (-149.123) * (-146.275) (-160.042) [-151.741] (-161.515) -- 0:02:10 Average standard deviation of split frequencies: 0.017557 316000 -- (-144.178) (-157.621) (-169.401) [-146.846] * (-146.960) (-157.250) [-146.741] (-164.144) -- 0:02:12 317000 -- (-145.382) (-160.632) (-169.345) [-153.854] * (-154.021) (-159.940) [-142.258] (-161.548) -- 0:02:11 318000 -- (-146.924) (-158.404) (-173.920) [-142.646] * [-148.651] (-159.500) (-148.272) (-163.394) -- 0:02:10 319000 -- (-151.900) (-158.546) (-178.550) [-144.598] * [-145.853] (-164.873) (-141.893) (-164.685) -- 0:02:10 320000 -- [-148.677] (-157.589) (-163.265) (-142.743) * [-143.958] (-159.199) (-155.102) (-164.383) -- 0:02:09 Average standard deviation of split frequencies: 0.017019 321000 -- (-149.112) [-152.677] (-171.380) (-144.865) * (-148.720) (-158.330) [-150.185] (-159.301) -- 0:02:11 322000 -- (-147.190) [-144.260] (-174.168) (-148.642) * [-138.518] (-165.636) (-149.523) (-157.240) -- 0:02:10 323000 -- (-148.531) [-147.305] (-165.182) (-157.840) * [-147.231] (-164.772) (-157.524) (-166.065) -- 0:02:09 324000 -- (-154.877) (-147.634) (-166.722) [-139.091] * (-139.821) (-166.327) [-144.225] (-155.064) -- 0:02:09 325000 -- (-149.320) [-138.337] (-165.233) (-144.767) * [-144.495] (-158.490) (-153.629) (-153.973) -- 0:02:08 Average standard deviation of split frequencies: 0.016629 326000 -- (-162.345) [-146.540] (-162.332) (-148.866) * (-152.308) (-159.065) [-155.852] (-159.372) -- 0:02:10 327000 -- (-154.770) (-150.733) [-146.872] (-141.526) * (-145.049) (-158.111) [-150.711] (-158.094) -- 0:02:09 328000 -- (-159.732) (-143.264) [-148.118] (-146.136) * (-146.334) (-164.031) [-144.551] (-163.812) -- 0:02:09 329000 -- (-159.789) (-144.338) (-158.206) [-145.069] * (-140.262) (-153.343) [-145.643] (-158.247) -- 0:02:08 330000 -- (-165.661) [-144.115] (-164.619) (-144.320) * [-146.354] (-158.339) (-144.224) (-160.947) -- 0:02:07 Average standard deviation of split frequencies: 0.016474 331000 -- (-163.373) (-150.039) (-157.678) [-142.745] * (-147.074) (-158.332) [-140.996] (-154.207) -- 0:02:09 332000 -- (-168.958) [-140.798] (-168.366) (-142.730) * (-152.309) (-154.227) [-149.618] (-160.375) -- 0:02:08 333000 -- (-162.072) (-151.280) (-160.410) [-151.872] * (-152.173) [-143.420] (-146.610) (-163.859) -- 0:02:08 334000 -- (-161.353) (-144.432) (-165.583) [-146.710] * (-149.373) (-149.147) [-145.790] (-155.289) -- 0:02:07 335000 -- (-161.415) (-141.792) (-155.705) [-144.182] * [-151.394] (-153.247) (-146.525) (-160.089) -- 0:02:07 Average standard deviation of split frequencies: 0.017483 336000 -- (-168.303) [-143.051] (-154.542) (-151.204) * [-144.964] (-147.090) (-147.963) (-152.787) -- 0:02:06 337000 -- (-168.680) [-146.926] (-161.837) (-154.829) * [-150.240] (-156.335) (-146.354) (-164.649) -- 0:02:07 338000 -- [-153.094] (-144.360) (-164.746) (-153.094) * (-147.872) [-147.746] (-152.104) (-156.159) -- 0:02:07 339000 -- (-151.281) (-154.712) (-161.613) [-151.417] * (-152.155) [-147.166] (-160.329) (-159.793) -- 0:02:06 340000 -- [-144.768] (-150.967) (-165.549) (-141.827) * (-142.189) [-144.615] (-145.145) (-171.155) -- 0:02:06 Average standard deviation of split frequencies: 0.017182 341000 -- (-143.960) (-143.904) (-160.452) [-144.603] * (-145.242) [-143.198] (-151.829) (-158.543) -- 0:02:05 342000 -- (-146.301) (-150.070) (-159.254) [-142.348] * [-141.279] (-149.052) (-160.679) (-159.636) -- 0:02:06 343000 -- (-149.004) (-146.601) (-160.804) [-144.003] * [-149.188] (-147.694) (-152.010) (-153.701) -- 0:02:06 344000 -- (-146.335) [-141.286] (-168.262) (-155.210) * (-154.620) (-148.315) [-150.713] (-158.765) -- 0:02:05 345000 -- (-141.726) [-138.937] (-160.361) (-155.246) * [-153.179] (-144.054) (-164.382) (-150.396) -- 0:02:05 Average standard deviation of split frequencies: 0.015946 346000 -- (-149.852) [-141.325] (-158.309) (-153.003) * [-145.146] (-145.837) (-154.500) (-159.149) -- 0:02:04 347000 -- [-145.009] (-145.130) (-165.241) (-148.601) * (-153.312) [-145.698] (-157.331) (-161.561) -- 0:02:06 348000 -- (-146.440) (-147.780) (-165.313) [-151.423] * (-145.491) [-141.768] (-152.753) (-159.347) -- 0:02:05 349000 -- [-146.041] (-145.377) (-163.287) (-145.911) * [-145.159] (-147.117) (-144.545) (-156.530) -- 0:02:04 350000 -- [-150.142] (-145.410) (-164.453) (-143.433) * [-143.393] (-151.723) (-151.529) (-160.907) -- 0:02:04 Average standard deviation of split frequencies: 0.017153 351000 -- (-151.461) (-150.679) (-156.353) [-154.691] * (-145.424) [-136.943] (-178.099) (-157.630) -- 0:02:03 352000 -- (-150.992) (-144.180) (-159.911) [-148.187] * (-146.844) [-140.202] (-165.360) (-153.590) -- 0:02:05 353000 -- [-147.399] (-145.562) (-164.153) (-146.648) * [-147.867] (-146.130) (-146.269) (-167.708) -- 0:02:04 354000 -- [-145.695] (-143.908) (-159.285) (-151.129) * [-147.791] (-143.766) (-167.765) (-163.071) -- 0:02:04 355000 -- (-148.672) [-144.931] (-154.115) (-159.034) * (-143.295) [-141.148] (-151.181) (-178.064) -- 0:02:03 Average standard deviation of split frequencies: 0.016599 356000 -- (-145.271) [-150.016] (-159.063) (-153.061) * (-151.427) (-142.348) [-144.472] (-162.644) -- 0:02:03 357000 -- [-147.742] (-141.050) (-161.414) (-150.404) * (-146.611) (-143.803) [-145.949] (-173.436) -- 0:02:02 358000 -- (-152.540) [-146.432] (-163.921) (-154.424) * (-152.548) (-142.714) [-145.784] (-160.060) -- 0:02:03 359000 -- (-144.480) [-141.893] (-156.978) (-151.369) * (-152.342) (-146.304) [-143.690] (-162.338) -- 0:02:03 360000 -- (-144.965) [-143.204] (-161.888) (-149.253) * (-164.136) (-144.482) [-142.953] (-163.162) -- 0:02:02 Average standard deviation of split frequencies: 0.016851 361000 -- [-147.626] (-143.320) (-156.183) (-148.787) * (-166.537) (-149.439) [-143.253] (-153.813) -- 0:02:02 362000 -- (-154.441) [-143.374] (-160.500) (-141.923) * (-160.073) (-152.256) [-142.623] (-166.201) -- 0:02:01 363000 -- (-150.886) (-150.152) (-160.446) [-142.372] * (-165.887) (-153.014) [-144.462] (-161.181) -- 0:02:02 364000 -- (-146.687) [-145.961] (-159.019) (-145.574) * (-157.067) (-148.855) [-149.118] (-162.976) -- 0:02:02 365000 -- (-152.340) (-152.805) (-160.930) [-142.400] * (-152.388) (-153.219) [-144.338] (-156.534) -- 0:02:01 Average standard deviation of split frequencies: 0.016422 366000 -- [-143.647] (-144.159) (-154.658) (-158.291) * (-159.509) (-143.618) [-147.091] (-162.199) -- 0:02:01 367000 -- [-143.910] (-155.037) (-152.971) (-152.194) * (-160.291) [-140.421] (-147.805) (-157.940) -- 0:02:00 368000 -- (-145.617) [-146.334] (-157.801) (-153.648) * (-153.653) (-145.251) [-148.309] (-160.110) -- 0:02:01 369000 -- [-150.065] (-144.459) (-155.408) (-149.480) * (-162.806) (-145.760) [-148.866] (-156.965) -- 0:02:01 370000 -- (-149.083) [-145.473] (-161.620) (-145.882) * (-163.215) [-144.166] (-147.407) (-158.772) -- 0:02:00 Average standard deviation of split frequencies: 0.016721 371000 -- (-149.945) (-144.790) (-152.415) [-141.800] * (-156.794) (-147.735) (-145.195) [-140.806] -- 0:02:00 372000 -- [-140.733] (-145.354) (-161.341) (-146.670) * (-153.481) (-141.538) (-160.556) [-147.621] -- 0:01:59 373000 -- (-145.319) [-151.890] (-161.746) (-145.718) * (-161.756) [-140.049] (-146.260) (-169.554) -- 0:02:01 374000 -- (-146.257) [-154.227] (-159.782) (-148.874) * (-164.250) (-146.597) [-147.773] (-153.497) -- 0:02:00 375000 -- (-144.461) (-156.553) (-155.907) [-143.675] * (-154.610) [-143.724] (-150.533) (-160.538) -- 0:02:00 Average standard deviation of split frequencies: 0.016829 376000 -- (-140.684) (-162.535) (-154.781) [-151.052] * (-156.631) [-145.414] (-146.572) (-161.336) -- 0:01:59 377000 -- [-142.462] (-164.213) (-160.278) (-147.764) * (-159.168) [-145.310] (-140.447) (-155.048) -- 0:01:58 378000 -- [-142.835] (-161.672) (-162.746) (-148.303) * (-169.744) [-143.317] (-144.077) (-161.128) -- 0:02:00 379000 -- (-146.309) (-157.970) (-165.813) [-140.174] * (-158.698) (-152.910) [-145.907] (-162.963) -- 0:01:59 380000 -- [-144.541] (-157.164) (-164.544) (-147.966) * (-149.905) [-144.842] (-147.740) (-166.081) -- 0:01:59 Average standard deviation of split frequencies: 0.016289 381000 -- (-149.451) (-157.499) (-147.025) [-151.703] * (-155.667) (-143.496) [-140.746] (-167.890) -- 0:01:58 382000 -- (-148.174) (-159.755) (-148.615) [-143.703] * (-152.683) [-145.701] (-147.426) (-159.595) -- 0:01:58 383000 -- (-150.971) (-158.845) (-157.441) [-141.645] * (-156.884) [-144.887] (-154.348) (-163.982) -- 0:01:57 384000 -- (-153.668) (-159.108) (-169.647) [-151.773] * (-160.733) (-145.729) [-147.411] (-159.499) -- 0:01:58 385000 -- [-148.351] (-159.510) (-159.055) (-154.029) * (-162.284) [-142.145] (-151.558) (-159.752) -- 0:01:58 Average standard deviation of split frequencies: 0.017278 386000 -- (-145.475) (-165.344) (-152.113) [-147.329] * (-161.443) [-147.190] (-145.430) (-161.091) -- 0:01:57 387000 -- (-148.618) (-157.433) (-159.537) [-148.673] * (-165.537) [-145.128] (-150.442) (-161.848) -- 0:01:57 388000 -- (-144.897) (-157.162) (-155.042) [-152.097] * (-152.131) [-146.152] (-155.416) (-156.530) -- 0:01:56 389000 -- (-143.623) (-151.185) (-154.460) [-150.629] * (-157.301) [-150.211] (-149.511) (-161.256) -- 0:01:57 390000 -- (-143.814) (-161.672) (-153.042) [-148.882] * (-159.353) [-148.460] (-150.196) (-165.156) -- 0:01:57 Average standard deviation of split frequencies: 0.016940 391000 -- [-144.584] (-160.482) (-160.900) (-148.515) * (-155.437) (-148.480) [-149.228] (-158.945) -- 0:01:56 392000 -- [-145.953] (-153.865) (-167.867) (-146.249) * (-159.443) [-147.149] (-143.768) (-164.819) -- 0:01:56 393000 -- (-149.825) (-152.893) (-161.851) [-145.251] * (-161.343) (-145.399) [-143.378] (-155.971) -- 0:01:55 394000 -- (-144.038) [-148.377] (-168.159) (-145.451) * [-146.034] (-151.513) (-148.752) (-158.543) -- 0:01:56 395000 -- (-150.123) [-143.763] (-151.528) (-151.547) * (-143.550) (-147.599) [-140.035] (-152.910) -- 0:01:56 Average standard deviation of split frequencies: 0.016666 396000 -- (-148.646) [-140.721] (-158.066) (-144.778) * (-141.410) [-145.082] (-151.996) (-150.410) -- 0:01:55 397000 -- (-151.459) [-145.341] (-158.843) (-155.344) * (-149.542) (-170.794) [-144.038] (-163.734) -- 0:01:55 398000 -- [-150.473] (-150.635) (-167.262) (-162.655) * (-149.688) (-169.286) [-152.827] (-159.497) -- 0:01:54 399000 -- (-145.312) [-145.890] (-158.091) (-159.478) * [-143.446] (-167.980) (-149.178) (-158.226) -- 0:01:55 400000 -- (-149.281) [-144.929] (-157.178) (-157.285) * [-144.765] (-164.894) (-147.876) (-157.893) -- 0:01:55 Average standard deviation of split frequencies: 0.016019 401000 -- (-154.104) [-146.954] (-160.429) (-149.476) * (-148.021) (-161.683) [-150.491] (-163.279) -- 0:01:55 402000 -- (-147.001) [-143.915] (-164.120) (-147.608) * (-141.597) (-166.223) (-154.034) [-145.023] -- 0:01:54 403000 -- [-144.616] (-150.674) (-164.187) (-159.828) * (-143.105) (-164.668) [-153.095] (-155.750) -- 0:01:54 404000 -- [-146.499] (-154.076) (-163.819) (-162.149) * [-148.589] (-153.198) (-148.245) (-158.009) -- 0:01:53 405000 -- [-142.599] (-144.009) (-157.648) (-164.523) * (-152.917) (-157.427) [-147.973] (-154.268) -- 0:01:54 Average standard deviation of split frequencies: 0.016747 406000 -- [-142.818] (-150.916) (-159.733) (-166.670) * (-149.322) (-161.678) [-148.197] (-153.156) -- 0:01:54 407000 -- [-143.620] (-142.453) (-158.403) (-166.188) * [-149.338] (-158.309) (-150.725) (-152.717) -- 0:01:53 408000 -- (-145.303) [-141.156] (-159.522) (-166.781) * [-148.444] (-162.056) (-143.568) (-162.318) -- 0:01:53 409000 -- (-143.158) [-146.314] (-153.375) (-164.314) * [-143.727] (-154.379) (-138.828) (-159.644) -- 0:01:52 410000 -- (-152.812) [-145.512] (-158.496) (-159.705) * [-148.019] (-160.279) (-150.544) (-155.415) -- 0:01:53 Average standard deviation of split frequencies: 0.016600 411000 -- [-141.916] (-144.042) (-164.953) (-162.709) * (-142.758) (-160.127) [-145.461] (-155.309) -- 0:01:53 412000 -- [-148.122] (-144.443) (-163.858) (-167.061) * [-141.291] (-165.298) (-145.214) (-167.572) -- 0:01:52 413000 -- [-139.307] (-142.968) (-158.641) (-167.414) * (-153.031) (-156.908) [-139.562] (-165.300) -- 0:01:52 414000 -- (-143.725) (-149.243) [-146.418] (-158.338) * (-148.124) (-161.544) [-142.633] (-157.056) -- 0:01:51 415000 -- (-151.255) [-139.784] (-148.519) (-161.532) * (-145.055) (-153.840) [-138.961] (-154.116) -- 0:01:52 Average standard deviation of split frequencies: 0.016736 416000 -- (-159.897) (-146.958) [-150.675] (-163.501) * (-148.878) (-167.706) [-141.620] (-155.344) -- 0:01:52 417000 -- (-171.374) [-141.885] (-148.142) (-165.317) * [-144.254] (-156.988) (-152.216) (-158.889) -- 0:01:51 418000 -- (-162.115) [-145.763] (-148.389) (-162.649) * [-148.397] (-155.819) (-151.240) (-156.191) -- 0:01:51 419000 -- (-149.597) [-139.545] (-151.531) (-157.429) * [-142.737] (-161.367) (-151.732) (-153.153) -- 0:01:50 420000 -- (-145.394) (-151.137) [-145.199] (-152.955) * [-146.459] (-157.851) (-144.446) (-157.959) -- 0:01:51 Average standard deviation of split frequencies: 0.016723 421000 -- [-148.887] (-145.820) (-145.270) (-158.629) * [-144.949] (-148.644) (-148.639) (-161.265) -- 0:01:51 422000 -- (-146.046) [-142.525] (-146.710) (-165.831) * [-141.476] (-162.081) (-152.370) (-163.610) -- 0:01:50 423000 -- [-149.758] (-160.272) (-146.098) (-159.111) * (-146.410) (-148.663) [-146.952] (-158.975) -- 0:01:50 424000 -- (-146.299) (-161.011) [-145.034] (-163.723) * (-143.712) (-153.372) [-151.544] (-159.961) -- 0:01:50 425000 -- (-150.778) [-149.362] (-152.455) (-162.801) * (-142.894) (-150.563) [-145.921] (-157.953) -- 0:01:50 Average standard deviation of split frequencies: 0.016173 426000 -- [-146.333] (-147.452) (-142.807) (-162.433) * (-144.265) (-166.608) [-143.985] (-153.214) -- 0:01:50 427000 -- [-146.373] (-156.385) (-150.501) (-158.428) * (-147.044) (-160.355) (-161.089) [-143.833] -- 0:01:50 428000 -- [-147.460] (-153.978) (-146.436) (-162.967) * [-142.758] (-161.201) (-161.939) (-152.205) -- 0:01:49 429000 -- (-147.213) [-147.606] (-150.678) (-158.752) * (-157.756) (-153.732) (-168.274) [-147.543] -- 0:01:49 430000 -- [-143.390] (-152.866) (-158.333) (-154.621) * (-149.455) (-163.548) (-156.532) [-144.220] -- 0:01:48 Average standard deviation of split frequencies: 0.015368 431000 -- (-157.357) (-149.107) [-145.479] (-159.688) * [-155.095] (-155.340) (-175.820) (-146.507) -- 0:01:49 432000 -- (-148.121) (-147.491) [-146.254] (-159.599) * (-154.463) [-146.110] (-163.195) (-146.293) -- 0:01:49 433000 -- [-147.849] (-148.445) (-155.735) (-160.672) * [-157.418] (-147.840) (-156.449) (-157.493) -- 0:01:48 434000 -- (-146.068) (-157.429) [-138.663] (-161.202) * (-148.484) [-148.724] (-156.787) (-156.839) -- 0:01:48 435000 -- [-146.866] (-147.488) (-144.658) (-157.250) * (-149.438) [-141.371] (-156.281) (-157.006) -- 0:01:47 Average standard deviation of split frequencies: 0.014887 436000 -- [-148.672] (-148.894) (-151.022) (-163.835) * (-146.100) [-145.587] (-154.181) (-168.208) -- 0:01:48 437000 -- (-153.109) [-148.457] (-145.625) (-160.180) * (-150.670) [-148.210] (-157.996) (-163.583) -- 0:01:48 438000 -- (-155.055) (-148.424) [-146.866] (-153.888) * [-147.984] (-155.134) (-152.644) (-155.761) -- 0:01:47 439000 -- (-155.519) (-149.747) [-152.102] (-155.750) * [-151.121] (-156.581) (-159.888) (-158.557) -- 0:01:47 440000 -- (-161.855) (-144.006) [-147.264] (-160.094) * (-154.902) [-143.974] (-163.317) (-159.270) -- 0:01:46 Average standard deviation of split frequencies: 0.015019 441000 -- (-157.831) [-143.558] (-148.101) (-152.543) * (-152.850) [-147.162] (-156.714) (-164.596) -- 0:01:47 442000 -- (-163.589) [-146.220] (-152.114) (-158.962) * [-152.567] (-148.187) (-155.046) (-158.623) -- 0:01:47 443000 -- (-155.319) (-150.066) [-147.850] (-156.823) * (-148.970) (-153.699) (-163.046) [-149.580] -- 0:01:46 444000 -- [-143.889] (-142.603) (-149.687) (-165.078) * [-149.963] (-147.547) (-157.122) (-159.147) -- 0:01:46 445000 -- [-144.821] (-146.077) (-140.128) (-166.514) * [-148.806] (-151.002) (-160.388) (-152.842) -- 0:01:46 Average standard deviation of split frequencies: 0.014716 446000 -- [-148.309] (-145.625) (-166.962) (-154.482) * (-148.896) [-141.356] (-161.942) (-147.723) -- 0:01:46 447000 -- [-138.528] (-138.405) (-161.417) (-151.243) * (-147.072) [-140.927] (-162.028) (-149.497) -- 0:01:46 448000 -- (-148.092) [-144.984] (-160.696) (-165.144) * (-154.945) [-140.481] (-151.197) (-142.000) -- 0:01:45 449000 -- (-148.079) (-145.431) (-170.297) [-150.131] * (-149.191) [-138.880] (-160.280) (-148.658) -- 0:01:45 450000 -- (-148.327) [-157.856] (-166.216) (-145.448) * (-152.851) [-149.452] (-155.240) (-154.320) -- 0:01:45 Average standard deviation of split frequencies: 0.014100 451000 -- (-160.904) (-145.125) (-161.470) [-149.126] * [-151.371] (-158.833) (-158.187) (-145.380) -- 0:01:44 452000 -- (-166.413) (-141.522) (-162.461) [-149.884] * (-151.465) [-141.861] (-161.950) (-143.377) -- 0:01:45 453000 -- (-166.211) [-145.191] (-160.124) (-156.640) * (-148.538) [-147.586] (-156.732) (-149.489) -- 0:01:45 454000 -- (-160.647) (-148.497) (-167.806) [-154.467] * (-151.523) (-156.625) (-156.223) [-141.447] -- 0:01:44 455000 -- (-160.225) (-149.947) (-162.612) [-150.772] * (-151.884) (-162.806) (-166.857) [-144.610] -- 0:01:44 Average standard deviation of split frequencies: 0.013935 456000 -- (-158.437) (-144.936) (-164.407) [-144.133] * (-147.455) (-151.153) (-162.562) [-141.094] -- 0:01:43 457000 -- (-164.033) [-147.163] (-158.860) (-142.017) * [-144.239] (-156.769) (-157.169) (-158.339) -- 0:01:44 458000 -- (-165.500) [-147.060] (-158.128) (-150.298) * [-147.226] (-156.572) (-162.295) (-149.556) -- 0:01:44 459000 -- (-160.938) [-142.342] (-159.376) (-150.293) * [-145.263] (-160.654) (-146.746) (-153.328) -- 0:01:43 460000 -- (-157.022) [-149.216] (-163.430) (-142.833) * [-148.405] (-169.173) (-146.956) (-148.886) -- 0:01:43 Average standard deviation of split frequencies: 0.013385 461000 -- (-157.476) (-150.540) (-164.783) [-147.195] * [-145.833] (-171.551) (-159.871) (-144.075) -- 0:01:42 462000 -- (-158.617) (-149.307) (-158.467) [-148.103] * [-146.778] (-158.243) (-163.284) (-139.559) -- 0:01:43 463000 -- (-156.307) [-145.171] (-162.368) (-145.746) * (-149.590) [-143.599] (-166.924) (-143.404) -- 0:01:43 464000 -- (-154.137) [-148.431] (-159.104) (-140.315) * (-146.593) (-139.751) (-162.411) [-141.261] -- 0:01:42 465000 -- (-161.381) (-151.905) (-159.320) [-140.318] * (-155.017) (-147.074) (-164.498) [-142.604] -- 0:01:42 Average standard deviation of split frequencies: 0.013345 466000 -- (-164.697) [-150.116] (-162.243) (-143.855) * (-160.357) [-141.982] (-165.652) (-144.205) -- 0:01:41 467000 -- (-169.178) [-146.403] (-150.394) (-151.966) * (-159.372) [-150.175] (-160.911) (-148.906) -- 0:01:42 468000 -- (-160.187) (-146.176) (-165.006) [-143.343] * (-165.179) [-146.797] (-155.693) (-155.436) -- 0:01:42 469000 -- (-163.869) [-148.458] (-158.885) (-149.159) * (-162.532) [-140.366] (-155.017) (-148.408) -- 0:01:41 470000 -- (-162.428) (-149.047) (-155.337) [-150.120] * (-157.266) (-145.590) (-151.970) [-146.106] -- 0:01:41 Average standard deviation of split frequencies: 0.013101 471000 -- (-154.463) (-146.562) (-155.822) [-144.565] * (-164.037) [-144.013] (-154.563) (-143.760) -- 0:01:41 472000 -- (-157.971) [-150.787] (-162.783) (-141.297) * (-161.069) [-149.643] (-165.373) (-146.172) -- 0:01:40 473000 -- (-159.258) [-150.627] (-160.108) (-147.774) * (-158.139) [-143.848] (-163.630) (-149.853) -- 0:01:41 474000 -- (-153.638) [-145.671] (-158.650) (-144.252) * (-174.275) [-146.570] (-156.778) (-141.669) -- 0:01:40 475000 -- (-152.904) (-165.737) (-140.355) [-145.315] * (-167.053) (-146.707) (-156.670) [-150.092] -- 0:01:40 Average standard deviation of split frequencies: 0.012914 476000 -- (-158.924) [-147.023] (-154.950) (-149.837) * (-162.460) [-145.599] (-147.258) (-152.716) -- 0:01:40 477000 -- (-159.993) (-138.269) [-152.130] (-150.678) * (-164.863) (-149.228) (-147.105) [-143.289] -- 0:01:39 478000 -- (-157.831) (-146.213) [-146.321] (-154.504) * [-146.846] (-146.031) (-150.188) (-164.483) -- 0:01:40 479000 -- (-164.820) [-147.209] (-146.473) (-144.353) * (-158.121) (-144.878) [-149.689] (-157.536) -- 0:01:40 480000 -- (-160.599) (-140.966) (-151.163) [-146.192] * (-162.554) (-149.645) (-150.940) [-149.630] -- 0:01:39 Average standard deviation of split frequencies: 0.012593 481000 -- (-159.121) [-141.413] (-171.636) (-146.029) * (-161.846) (-142.502) (-144.257) [-143.853] -- 0:01:39 482000 -- (-156.749) (-147.499) (-151.336) [-148.049] * (-160.873) [-142.882] (-149.120) (-141.123) -- 0:01:38 483000 -- (-152.684) (-163.045) (-146.848) [-146.177] * (-159.957) (-149.932) (-145.121) [-140.037] -- 0:01:39 484000 -- (-150.465) (-172.703) (-147.851) [-146.854] * (-154.504) (-149.555) (-143.147) [-143.323] -- 0:01:39 485000 -- (-157.047) (-165.652) [-143.665] (-144.261) * (-155.181) (-143.467) (-155.032) [-146.669] -- 0:01:38 Average standard deviation of split frequencies: 0.012726 486000 -- (-157.806) (-158.829) (-143.932) [-148.422] * (-160.765) (-154.274) (-165.082) [-141.787] -- 0:01:38 487000 -- (-150.300) (-163.762) (-145.532) [-148.572] * (-155.515) [-146.570] (-168.194) (-148.132) -- 0:01:37 488000 -- (-159.942) (-160.239) [-140.404] (-141.283) * (-160.890) [-147.685] (-160.771) (-145.097) -- 0:01:38 489000 -- (-154.053) (-150.246) (-143.855) [-148.633] * (-156.179) [-146.091] (-160.518) (-143.454) -- 0:01:38 490000 -- (-158.906) (-154.359) [-146.271] (-158.485) * (-163.588) [-146.134] (-160.032) (-147.914) -- 0:01:37 Average standard deviation of split frequencies: 0.012912 491000 -- (-161.094) (-157.735) (-145.231) [-145.011] * (-157.711) (-142.086) (-165.991) [-147.341] -- 0:01:37 492000 -- (-161.163) (-151.840) [-143.477] (-147.934) * (-155.770) [-142.408] (-155.963) (-145.336) -- 0:01:37 493000 -- (-166.017) (-159.785) [-141.532] (-145.899) * (-161.767) (-141.790) (-156.804) [-138.058] -- 0:01:37 494000 -- (-169.794) (-160.384) [-144.593] (-144.168) * (-163.561) [-148.026] (-171.824) (-140.844) -- 0:01:37 495000 -- (-167.847) (-157.145) [-143.915] (-143.271) * (-162.037) (-151.237) (-164.796) [-145.223] -- 0:01:36 Average standard deviation of split frequencies: 0.012633 496000 -- (-170.344) (-156.349) (-141.105) [-144.845] * (-164.546) [-150.661] (-171.861) (-143.483) -- 0:01:36 497000 -- (-162.628) (-150.362) [-143.510] (-146.615) * (-171.531) (-150.258) (-164.943) [-144.164] -- 0:01:36 498000 -- (-173.141) (-161.898) [-147.995] (-139.223) * (-158.576) (-152.398) (-160.091) [-147.102] -- 0:01:35 499000 -- (-163.356) (-157.409) (-141.277) [-144.614] * (-160.195) (-156.494) (-158.611) [-148.540] -- 0:01:36 500000 -- (-168.740) (-153.076) [-150.123] (-152.016) * (-155.581) (-147.074) (-156.210) [-141.528] -- 0:01:36 Average standard deviation of split frequencies: 0.012316 501000 -- (-170.047) (-148.839) [-146.076] (-148.115) * (-156.704) [-148.268] (-158.713) (-145.671) -- 0:01:35 502000 -- (-159.525) (-157.291) [-143.181] (-144.984) * (-153.664) (-144.391) (-164.430) [-143.598] -- 0:01:35 503000 -- (-168.404) (-162.103) (-145.510) [-147.109] * (-162.052) [-145.440] (-157.534) (-140.116) -- 0:01:34 504000 -- (-170.123) (-157.237) [-144.217] (-144.900) * (-163.810) (-146.690) (-156.105) [-145.765] -- 0:01:35 505000 -- (-163.126) (-162.357) [-143.134] (-142.075) * (-155.376) (-152.085) (-160.069) [-153.810] -- 0:01:35 Average standard deviation of split frequencies: 0.012223 506000 -- (-152.728) (-165.889) (-142.024) [-149.413] * (-164.149) (-158.169) (-160.413) [-146.000] -- 0:01:34 507000 -- (-168.446) (-157.180) (-144.942) [-142.615] * (-157.210) [-151.355] (-153.151) (-147.940) -- 0:01:34 508000 -- (-162.706) (-159.474) (-148.735) [-144.944] * (-161.547) (-152.476) [-147.562] (-142.259) -- 0:01:33 509000 -- (-154.505) (-162.423) [-146.039] (-144.520) * (-152.502) (-145.462) [-144.188] (-141.793) -- 0:01:34 510000 -- (-156.731) (-154.349) (-146.935) [-138.960] * (-168.488) (-152.834) (-148.833) [-141.676] -- 0:01:34 Average standard deviation of split frequencies: 0.012462 511000 -- (-166.431) (-160.142) (-149.508) [-141.030] * (-158.599) (-154.391) (-148.487) [-142.496] -- 0:01:33 512000 -- (-162.171) (-160.204) [-151.974] (-147.627) * (-159.216) (-150.206) (-146.027) [-149.180] -- 0:01:33 513000 -- (-174.526) (-146.908) (-147.847) [-151.424] * (-167.020) [-149.893] (-154.845) (-139.491) -- 0:01:33 514000 -- (-162.138) (-144.884) (-147.278) [-139.785] * (-160.693) (-149.236) [-143.503] (-146.615) -- 0:01:33 515000 -- (-157.623) [-141.197] (-148.435) (-148.281) * (-165.441) (-161.865) [-148.301] (-147.022) -- 0:01:33 Average standard deviation of split frequencies: 0.011139 516000 -- (-164.307) [-143.463] (-151.221) (-151.931) * (-160.306) (-156.291) [-146.540] (-140.654) -- 0:01:32 517000 -- (-168.027) (-146.107) (-154.861) [-145.074] * (-160.460) (-162.395) (-151.613) [-144.473] -- 0:01:32 518000 -- (-157.513) [-137.738] (-163.132) (-144.094) * (-161.073) (-161.748) [-151.344] (-144.944) -- 0:01:32 519000 -- (-162.658) [-149.670] (-158.766) (-149.530) * (-160.362) (-158.251) (-155.241) [-147.769] -- 0:01:32 520000 -- (-161.561) (-151.496) (-153.922) [-144.354] * (-161.716) [-153.424] (-150.645) (-148.206) -- 0:01:32 Average standard deviation of split frequencies: 0.011372 521000 -- (-151.630) (-148.813) (-158.314) [-145.619] * (-164.403) [-145.302] (-147.614) (-148.437) -- 0:01:31 522000 -- (-155.299) [-144.735] (-162.422) (-146.165) * (-164.397) (-148.895) [-146.274] (-153.024) -- 0:01:31 523000 -- (-163.573) [-145.936] (-155.485) (-139.776) * (-163.276) (-147.968) [-141.733] (-150.421) -- 0:01:31 524000 -- (-159.843) (-143.309) (-156.521) [-142.099] * (-157.604) (-145.664) (-146.404) [-147.046] -- 0:01:31 525000 -- (-164.778) [-146.854] (-158.953) (-146.775) * (-157.094) [-147.253] (-147.998) (-149.369) -- 0:01:31 Average standard deviation of split frequencies: 0.011478 526000 -- (-157.899) [-142.305] (-168.196) (-144.915) * (-161.024) [-146.277] (-148.494) (-157.676) -- 0:01:31 527000 -- (-158.435) (-152.868) (-160.408) [-144.663] * (-159.226) (-147.921) [-144.513] (-161.152) -- 0:01:30 528000 -- (-159.246) [-143.788] (-167.177) (-147.658) * (-154.616) (-145.284) [-144.784] (-147.000) -- 0:01:30 529000 -- (-158.979) [-149.604] (-162.957) (-147.751) * (-154.834) [-146.130] (-143.348) (-163.838) -- 0:01:30 530000 -- (-159.050) [-147.097] (-161.351) (-155.453) * (-158.000) [-145.933] (-146.550) (-162.640) -- 0:01:30 Average standard deviation of split frequencies: 0.010797 531000 -- (-157.161) [-141.037] (-159.010) (-153.152) * (-154.663) (-147.049) [-142.512] (-162.644) -- 0:01:30 532000 -- (-170.613) (-142.005) (-155.490) [-144.475] * (-159.599) (-152.381) [-142.343] (-150.019) -- 0:01:29 533000 -- (-172.282) [-144.842] (-164.821) (-143.571) * (-155.810) (-148.499) [-140.718] (-156.309) -- 0:01:29 534000 -- (-166.797) (-147.824) (-165.196) [-152.757] * (-145.504) (-150.501) [-147.076] (-158.784) -- 0:01:29 535000 -- (-155.312) [-139.469] (-159.273) (-146.881) * (-144.866) [-146.109] (-146.267) (-164.482) -- 0:01:29 Average standard deviation of split frequencies: 0.010351 536000 -- (-162.965) (-143.434) (-162.471) [-152.560] * (-149.258) (-150.896) [-148.540] (-162.578) -- 0:01:29 537000 -- (-154.743) [-140.068] (-163.021) (-139.207) * (-151.042) (-150.342) [-145.827] (-160.712) -- 0:01:28 538000 -- (-154.727) (-143.189) (-159.202) [-141.777] * [-148.491] (-146.771) (-147.453) (-161.123) -- 0:01:28 539000 -- (-164.594) [-147.363] (-159.851) (-150.405) * [-145.495] (-144.028) (-156.162) (-157.964) -- 0:01:28 540000 -- (-162.484) (-147.115) (-164.033) [-143.939] * [-141.871] (-145.998) (-164.385) (-161.043) -- 0:01:28 Average standard deviation of split frequencies: 0.010295 541000 -- (-160.018) [-141.804] (-162.447) (-144.027) * (-147.225) [-144.106] (-164.851) (-157.685) -- 0:01:28 542000 -- (-166.810) [-145.852] (-160.951) (-146.215) * [-143.382] (-142.747) (-155.281) (-161.655) -- 0:01:27 543000 -- (-175.830) [-145.628] (-163.139) (-144.292) * [-140.034] (-157.087) (-159.388) (-149.552) -- 0:01:27 544000 -- (-161.334) [-145.303] (-154.750) (-153.117) * [-144.814] (-147.471) (-158.833) (-156.836) -- 0:01:27 545000 -- (-169.458) [-145.214] (-155.401) (-143.205) * (-145.129) [-144.436] (-154.465) (-163.802) -- 0:01:27 Average standard deviation of split frequencies: 0.009829 546000 -- (-161.018) (-144.530) (-158.913) [-145.609] * [-144.418] (-142.652) (-162.970) (-152.096) -- 0:01:27 547000 -- (-163.434) (-158.097) (-156.585) [-145.805] * (-149.595) [-150.245] (-157.794) (-158.491) -- 0:01:26 548000 -- (-158.913) (-147.584) (-157.061) [-142.740] * (-144.236) [-151.936] (-153.548) (-168.041) -- 0:01:26 549000 -- (-155.358) [-143.856] (-162.857) (-143.429) * [-145.403] (-143.674) (-160.843) (-160.550) -- 0:01:26 550000 -- (-156.090) [-147.217] (-164.806) (-151.251) * (-140.937) [-143.097] (-165.210) (-156.565) -- 0:01:26 Average standard deviation of split frequencies: 0.009581 551000 -- (-166.158) (-144.909) (-158.847) [-151.077] * [-148.913] (-146.291) (-145.141) (-161.812) -- 0:01:26 552000 -- (-164.462) [-151.125] (-159.968) (-158.130) * [-140.335] (-142.720) (-161.756) (-157.326) -- 0:01:26 553000 -- (-158.225) [-140.900] (-153.732) (-157.643) * [-146.071] (-149.403) (-153.119) (-163.236) -- 0:01:25 554000 -- (-163.133) [-157.418] (-144.360) (-161.472) * (-153.810) [-143.193] (-154.041) (-165.470) -- 0:01:25 555000 -- (-161.940) [-149.665] (-143.936) (-166.698) * (-151.059) [-140.195] (-145.815) (-159.762) -- 0:01:25 Average standard deviation of split frequencies: 0.009652 556000 -- (-160.590) [-141.276] (-153.833) (-161.008) * (-151.764) [-149.009] (-146.307) (-159.253) -- 0:01:25 557000 -- (-170.925) [-140.102] (-151.693) (-159.249) * (-165.375) [-151.572] (-156.038) (-162.437) -- 0:01:25 558000 -- (-165.050) [-139.804] (-150.610) (-159.239) * (-163.196) [-147.552] (-143.249) (-155.514) -- 0:01:24 559000 -- (-161.154) (-149.298) [-148.507] (-171.659) * (-164.270) (-152.946) [-147.138] (-164.165) -- 0:01:24 560000 -- (-156.369) (-141.706) [-147.690] (-161.610) * (-176.236) (-143.865) (-141.755) [-143.441] -- 0:01:24 Average standard deviation of split frequencies: 0.009518 561000 -- (-157.454) (-142.902) [-143.919] (-162.406) * (-169.715) (-163.198) [-148.290] (-154.562) -- 0:01:24 562000 -- [-146.725] (-158.613) (-151.131) (-155.756) * (-164.848) (-145.514) [-142.812] (-150.112) -- 0:01:24 563000 -- [-152.232] (-143.641) (-149.511) (-163.190) * (-168.482) (-145.037) (-144.795) [-143.025] -- 0:01:23 564000 -- [-143.512] (-148.851) (-166.206) (-158.554) * (-160.883) (-144.754) (-143.817) [-144.960] -- 0:01:23 565000 -- (-147.400) (-154.282) [-143.723] (-157.350) * (-165.230) (-148.532) [-143.239] (-148.822) -- 0:01:23 Average standard deviation of split frequencies: 0.009065 566000 -- [-145.686] (-158.017) (-152.486) (-166.036) * (-167.292) (-145.427) [-144.224] (-145.548) -- 0:01:23 567000 -- (-150.407) (-155.347) [-144.787] (-162.453) * (-166.322) [-139.734] (-143.182) (-143.477) -- 0:01:23 568000 -- [-149.620] (-165.805) (-148.163) (-164.189) * (-157.611) [-143.807] (-149.079) (-149.837) -- 0:01:22 569000 -- (-157.095) (-162.256) [-148.860] (-166.200) * (-159.719) (-141.194) [-145.695] (-147.080) -- 0:01:22 570000 -- [-147.507] (-161.966) (-155.966) (-147.670) * (-162.339) (-154.566) [-149.141] (-159.131) -- 0:01:22 Average standard deviation of split frequencies: 0.008811 571000 -- (-148.134) (-169.158) [-151.256] (-145.595) * (-166.214) (-144.638) [-146.779] (-160.291) -- 0:01:22 572000 -- (-152.460) (-157.105) (-156.544) [-149.192] * (-160.335) (-146.389) [-147.207] (-164.312) -- 0:01:22 573000 -- (-149.832) (-152.929) [-149.606] (-149.569) * (-154.359) (-150.643) [-152.578] (-164.481) -- 0:01:21 574000 -- [-146.893] (-160.335) (-153.682) (-154.134) * (-162.426) [-151.041] (-145.456) (-161.327) -- 0:01:21 575000 -- (-149.484) (-158.851) [-143.944] (-159.351) * (-160.066) [-148.364] (-145.942) (-161.592) -- 0:01:21 Average standard deviation of split frequencies: 0.008806 576000 -- (-144.146) (-158.378) [-149.560] (-156.044) * (-155.005) [-144.613] (-142.630) (-159.447) -- 0:01:21 577000 -- (-147.441) (-164.033) [-144.467] (-156.906) * (-154.937) (-144.742) [-142.765] (-160.767) -- 0:01:21 578000 -- (-151.248) (-163.348) [-140.601] (-156.819) * (-165.772) [-146.023] (-142.154) (-157.499) -- 0:01:21 579000 -- [-143.099] (-160.915) (-155.226) (-161.860) * [-141.407] (-151.978) (-137.556) (-156.971) -- 0:01:20 580000 -- (-152.892) (-153.472) [-140.389] (-165.442) * (-147.491) (-156.013) [-150.255] (-154.625) -- 0:01:20 Average standard deviation of split frequencies: 0.009235 581000 -- (-142.547) (-167.464) [-144.042] (-153.286) * [-142.191] (-143.766) (-154.149) (-163.360) -- 0:01:20 582000 -- (-148.072) (-159.380) [-145.073] (-158.497) * [-143.094] (-143.045) (-144.162) (-160.513) -- 0:01:20 583000 -- [-146.272] (-174.248) (-145.642) (-168.772) * (-146.385) [-144.990] (-145.191) (-164.791) -- 0:01:20 584000 -- [-148.662] (-156.959) (-143.075) (-156.835) * (-149.817) [-152.596] (-149.614) (-163.065) -- 0:01:19 585000 -- (-141.500) (-145.969) [-141.404] (-163.480) * (-165.307) [-150.675] (-146.910) (-174.095) -- 0:01:19 Average standard deviation of split frequencies: 0.008681 586000 -- [-145.322] (-153.069) (-142.549) (-161.408) * (-152.431) (-144.280) [-148.234] (-164.450) -- 0:01:19 587000 -- (-149.550) (-145.218) [-143.885] (-152.690) * (-158.705) (-147.558) [-142.968] (-164.580) -- 0:01:19 588000 -- (-146.957) (-147.845) [-144.187] (-158.212) * (-164.636) [-153.229] (-152.413) (-160.667) -- 0:01:19 589000 -- (-149.877) [-148.492] (-150.061) (-156.573) * (-164.610) [-137.726] (-152.820) (-164.076) -- 0:01:18 590000 -- [-144.702] (-158.766) (-150.947) (-154.533) * (-172.970) (-143.448) [-144.558] (-164.509) -- 0:01:18 Average standard deviation of split frequencies: 0.008646 591000 -- [-140.354] (-158.847) (-152.035) (-157.281) * (-150.438) (-148.197) [-145.671] (-159.311) -- 0:01:18 592000 -- [-142.746] (-160.455) (-147.240) (-158.461) * [-143.879] (-146.242) (-155.361) (-171.760) -- 0:01:18 593000 -- (-143.895) (-159.509) [-150.902] (-144.352) * [-151.928] (-147.762) (-160.768) (-156.059) -- 0:01:18 594000 -- (-151.242) (-165.944) [-144.323] (-152.625) * [-148.104] (-145.989) (-169.965) (-156.775) -- 0:01:17 595000 -- (-142.813) (-167.275) [-149.510] (-151.067) * [-146.764] (-149.570) (-154.499) (-163.701) -- 0:01:17 Average standard deviation of split frequencies: 0.008931 596000 -- (-155.055) (-157.441) [-141.259] (-162.037) * (-147.966) [-147.823] (-152.944) (-158.995) -- 0:01:17 597000 -- [-156.203] (-154.082) (-145.316) (-151.547) * (-151.919) [-140.721] (-159.649) (-157.380) -- 0:01:17 598000 -- (-150.199) (-157.235) [-141.387] (-156.138) * [-144.011] (-148.170) (-162.212) (-155.874) -- 0:01:17 599000 -- (-144.364) (-160.813) [-142.109] (-150.480) * (-144.599) [-150.830] (-157.960) (-161.469) -- 0:01:16 600000 -- (-140.740) (-157.561) [-150.737] (-167.106) * (-144.336) [-148.683] (-154.403) (-156.229) -- 0:01:16 Average standard deviation of split frequencies: 0.009254 601000 -- [-142.830] (-152.610) (-147.583) (-155.956) * (-147.806) (-150.472) [-152.421] (-159.694) -- 0:01:16 602000 -- [-144.977] (-172.520) (-148.033) (-162.049) * (-150.079) (-157.989) [-141.683] (-159.602) -- 0:01:16 603000 -- (-147.808) (-149.457) [-141.508] (-151.183) * (-156.880) (-161.951) [-144.197] (-158.842) -- 0:01:16 604000 -- [-140.368] (-150.393) (-143.599) (-160.267) * (-147.961) (-162.487) [-142.178] (-163.978) -- 0:01:16 605000 -- [-142.094] (-144.660) (-149.577) (-158.239) * [-148.208] (-159.975) (-147.652) (-170.387) -- 0:01:15 Average standard deviation of split frequencies: 0.008492 606000 -- [-145.884] (-147.698) (-140.442) (-157.000) * (-151.001) (-162.370) [-145.669] (-166.385) -- 0:01:15 607000 -- [-147.478] (-149.648) (-140.187) (-153.801) * [-148.394] (-167.142) (-142.007) (-143.512) -- 0:01:15 608000 -- (-143.842) [-138.507] (-143.251) (-158.431) * (-149.862) (-167.041) (-149.571) [-148.088] -- 0:01:15 609000 -- [-145.679] (-145.506) (-147.854) (-158.798) * (-145.563) (-157.726) (-157.018) [-157.870] -- 0:01:15 610000 -- [-149.804] (-143.363) (-153.242) (-157.287) * [-146.449] (-160.617) (-160.320) (-144.400) -- 0:01:14 Average standard deviation of split frequencies: 0.008105 611000 -- (-146.306) [-143.072] (-141.442) (-156.181) * (-156.762) [-148.098] (-158.510) (-146.886) -- 0:01:14 612000 -- [-145.247] (-140.582) (-156.748) (-151.818) * [-143.523] (-156.107) (-159.454) (-154.251) -- 0:01:14 613000 -- (-164.482) (-144.389) [-143.338] (-159.975) * [-147.940] (-155.492) (-160.227) (-147.123) -- 0:01:14 614000 -- (-161.566) [-150.638] (-146.448) (-160.529) * (-144.316) [-150.930] (-157.584) (-141.554) -- 0:01:14 615000 -- (-168.463) [-149.924] (-146.405) (-156.489) * (-154.165) (-144.107) (-158.278) [-141.023] -- 0:01:13 Average standard deviation of split frequencies: 0.008259 616000 -- (-163.034) [-147.998] (-146.801) (-157.550) * (-157.318) (-153.394) (-163.563) [-144.735] -- 0:01:13 617000 -- (-157.535) (-147.756) [-142.148] (-157.909) * (-144.826) (-157.585) (-157.989) [-150.947] -- 0:01:13 618000 -- (-159.794) (-148.664) [-145.564] (-144.164) * [-143.006] (-146.119) (-160.988) (-165.566) -- 0:01:13 619000 -- (-154.544) [-146.597] (-141.945) (-148.900) * (-143.220) [-146.558] (-159.703) (-151.366) -- 0:01:13 620000 -- (-161.097) (-146.700) (-145.651) [-143.506] * [-139.163] (-144.613) (-155.978) (-153.948) -- 0:01:12 Average standard deviation of split frequencies: 0.008133 621000 -- (-155.091) (-147.893) (-148.711) [-143.236] * [-142.070] (-152.754) (-161.225) (-164.279) -- 0:01:12 622000 -- (-160.883) (-146.374) (-149.211) [-145.998] * (-144.393) [-148.160] (-158.954) (-148.821) -- 0:01:12 623000 -- (-152.771) [-144.786] (-154.959) (-148.633) * (-153.565) [-148.247] (-168.204) (-150.535) -- 0:01:12 624000 -- (-159.762) (-147.760) (-146.392) [-145.275] * [-145.485] (-149.901) (-156.049) (-154.194) -- 0:01:12 625000 -- (-164.813) (-152.362) [-147.107] (-147.748) * [-149.131] (-151.928) (-166.586) (-158.458) -- 0:01:12 Average standard deviation of split frequencies: 0.008103 626000 -- (-167.629) (-140.026) (-151.322) [-144.801] * (-147.661) [-144.152] (-153.198) (-150.314) -- 0:01:11 627000 -- (-157.532) (-143.324) (-143.799) [-141.166] * [-147.574] (-145.151) (-154.757) (-155.972) -- 0:01:11 628000 -- (-159.341) [-142.415] (-144.355) (-152.532) * (-145.085) [-142.804] (-150.295) (-159.848) -- 0:01:11 629000 -- (-160.694) [-141.172] (-148.493) (-152.322) * [-146.416] (-145.694) (-163.043) (-161.453) -- 0:01:11 630000 -- (-160.563) [-143.554] (-143.491) (-153.197) * (-150.920) [-144.360] (-153.185) (-164.099) -- 0:01:11 Average standard deviation of split frequencies: 0.008162 631000 -- (-156.388) (-149.883) [-145.312] (-151.985) * [-143.431] (-153.145) (-162.607) (-158.353) -- 0:01:10 632000 -- (-163.138) [-142.608] (-149.706) (-162.017) * (-145.434) [-151.417] (-165.273) (-158.662) -- 0:01:10 633000 -- (-153.054) (-149.268) [-142.258] (-164.731) * [-141.936] (-150.137) (-151.133) (-159.941) -- 0:01:10 634000 -- (-160.648) [-142.911] (-144.937) (-147.622) * [-151.631] (-151.285) (-154.464) (-166.703) -- 0:01:10 635000 -- (-155.845) [-149.864] (-145.958) (-158.853) * [-140.026] (-157.421) (-157.753) (-154.393) -- 0:01:10 Average standard deviation of split frequencies: 0.007709 636000 -- (-166.226) [-141.722] (-144.280) (-152.580) * (-146.155) (-149.761) (-164.255) [-149.845] -- 0:01:09 637000 -- (-157.298) (-149.180) [-143.792] (-165.380) * (-145.694) (-142.829) (-164.315) [-147.267] -- 0:01:09 638000 -- (-163.470) [-144.546] (-145.155) (-158.631) * [-146.215] (-146.501) (-159.806) (-157.534) -- 0:01:09 639000 -- (-156.595) (-148.885) [-150.608] (-157.487) * (-146.656) (-150.486) (-152.243) [-148.067] -- 0:01:09 640000 -- (-160.059) [-142.817] (-146.711) (-157.785) * (-152.977) [-147.994] (-158.414) (-170.227) -- 0:01:09 Average standard deviation of split frequencies: 0.007389 641000 -- (-156.068) (-145.886) [-145.800] (-161.150) * [-144.795] (-149.712) (-155.581) (-161.146) -- 0:01:08 642000 -- (-158.816) (-145.200) [-149.856] (-158.276) * [-145.538] (-156.834) (-150.187) (-157.556) -- 0:01:08 643000 -- (-160.595) (-143.897) [-150.622] (-151.812) * [-147.389] (-143.147) (-155.417) (-162.096) -- 0:01:08 644000 -- (-162.610) [-142.095] (-152.256) (-158.975) * (-152.683) [-145.070] (-151.624) (-156.550) -- 0:01:08 645000 -- (-157.213) [-143.673] (-140.882) (-156.748) * [-142.302] (-145.791) (-148.135) (-159.946) -- 0:01:08 Average standard deviation of split frequencies: 0.007073 646000 -- (-158.634) [-143.350] (-148.971) (-162.195) * (-146.388) (-148.691) [-143.261] (-161.151) -- 0:01:07 647000 -- (-163.537) [-143.657] (-147.975) (-157.009) * [-151.087] (-144.334) (-151.222) (-161.560) -- 0:01:07 648000 -- (-156.416) [-149.361] (-143.185) (-154.628) * (-144.257) (-146.385) [-149.947] (-161.394) -- 0:01:07 649000 -- (-159.992) (-152.279) [-142.590] (-148.980) * [-142.930] (-149.562) (-148.119) (-156.274) -- 0:01:07 650000 -- (-167.062) (-147.684) [-147.084] (-155.998) * [-147.535] (-144.870) (-145.676) (-156.668) -- 0:01:07 Average standard deviation of split frequencies: 0.006128 651000 -- (-153.892) [-142.910] (-149.515) (-155.638) * (-148.027) (-152.910) [-146.030] (-158.935) -- 0:01:07 652000 -- (-168.107) [-142.921] (-141.168) (-150.137) * [-143.287] (-148.487) (-169.467) (-157.703) -- 0:01:06 653000 -- (-160.621) (-142.269) [-143.527] (-146.426) * (-142.601) [-152.055] (-162.308) (-164.184) -- 0:01:06 654000 -- (-164.433) [-150.601] (-156.364) (-144.398) * [-143.790] (-145.036) (-151.181) (-161.615) -- 0:01:06 655000 -- (-155.158) (-152.129) [-141.370] (-150.490) * [-143.612] (-151.614) (-157.485) (-152.413) -- 0:01:06 Average standard deviation of split frequencies: 0.005749 656000 -- (-161.067) (-149.245) [-144.562] (-160.223) * (-142.720) [-144.927] (-158.312) (-160.998) -- 0:01:06 657000 -- (-160.239) (-152.129) [-147.289] (-158.670) * [-147.197] (-160.055) (-159.526) (-154.408) -- 0:01:05 658000 -- (-153.103) (-152.555) (-151.211) [-141.364] * (-150.237) [-153.560] (-169.511) (-161.210) -- 0:01:05 659000 -- (-162.117) [-139.906] (-145.410) (-147.658) * [-146.918] (-151.562) (-148.974) (-157.650) -- 0:01:05 660000 -- (-170.773) (-152.401) [-151.416] (-139.754) * (-155.727) [-160.385] (-155.433) (-158.711) -- 0:01:05 Average standard deviation of split frequencies: 0.006079 661000 -- (-158.770) (-143.709) [-142.204] (-149.066) * (-149.982) (-164.722) [-147.167] (-154.650) -- 0:01:05 662000 -- (-162.627) [-148.863] (-143.768) (-146.466) * (-148.135) (-154.558) [-144.894] (-158.699) -- 0:01:04 663000 -- (-154.775) (-147.802) (-150.829) [-143.565] * (-143.262) (-157.239) [-144.916] (-155.412) -- 0:01:04 664000 -- (-160.161) [-140.767] (-150.962) (-143.169) * [-145.900] (-160.619) (-147.240) (-158.266) -- 0:01:04 665000 -- (-164.183) (-150.635) (-155.323) [-145.716] * [-145.257] (-160.692) (-152.963) (-157.469) -- 0:01:04 Average standard deviation of split frequencies: 0.005747 666000 -- (-158.290) (-145.947) (-164.395) [-141.117] * [-147.753] (-158.038) (-142.866) (-144.528) -- 0:01:04 667000 -- (-158.916) (-149.673) (-156.027) [-142.222] * (-143.683) (-162.978) [-141.090] (-157.015) -- 0:01:03 668000 -- (-154.339) (-145.161) (-167.895) [-140.170] * [-146.243] (-157.008) (-143.868) (-153.620) -- 0:01:03 669000 -- (-148.226) [-149.135] (-158.466) (-146.906) * (-154.430) (-166.670) (-168.951) [-145.988] -- 0:01:03 670000 -- (-148.394) [-153.834] (-154.779) (-161.380) * [-143.875] (-163.997) (-156.816) (-152.470) -- 0:01:03 Average standard deviation of split frequencies: 0.006434 671000 -- (-156.868) (-146.857) (-160.040) [-141.302] * [-148.002] (-162.692) (-173.246) (-146.386) -- 0:01:03 672000 -- (-157.636) (-151.389) (-155.361) [-142.822] * [-151.528] (-159.966) (-158.183) (-143.100) -- 0:01:02 673000 -- (-149.742) (-139.885) (-160.714) [-152.658] * [-144.947] (-143.888) (-159.491) (-144.484) -- 0:01:02 674000 -- (-163.600) [-148.048] (-152.806) (-150.243) * (-145.215) [-142.957] (-157.852) (-151.356) -- 0:01:02 675000 -- (-155.980) [-141.829] (-160.954) (-145.451) * (-144.285) [-144.575] (-158.827) (-157.852) -- 0:01:02 Average standard deviation of split frequencies: 0.007085 676000 -- (-146.767) (-155.798) (-159.241) [-148.469] * (-153.490) [-145.372] (-165.155) (-150.100) -- 0:01:02 677000 -- (-145.821) (-147.358) (-160.980) [-146.085] * (-141.073) (-155.016) (-161.015) [-149.052] -- 0:01:02 678000 -- (-144.870) (-143.107) (-156.325) [-141.015] * (-145.337) (-149.015) (-160.525) [-152.739] -- 0:01:01 679000 -- (-164.239) (-147.337) [-154.067] (-151.719) * [-145.459] (-147.361) (-156.545) (-156.561) -- 0:01:01 680000 -- (-144.977) [-144.595] (-149.398) (-152.417) * (-155.435) [-143.212] (-157.655) (-162.873) -- 0:01:01 Average standard deviation of split frequencies: 0.006649 681000 -- [-146.440] (-145.515) (-146.045) (-163.951) * (-156.094) [-153.363] (-159.493) (-153.268) -- 0:01:01 682000 -- (-147.359) [-144.268] (-148.033) (-153.938) * (-146.887) (-150.112) (-161.723) [-153.608] -- 0:01:01 683000 -- [-145.335] (-143.547) (-143.338) (-156.092) * (-152.605) [-149.756] (-159.976) (-149.419) -- 0:01:00 684000 -- [-144.110] (-144.485) (-150.847) (-166.270) * [-154.713] (-151.598) (-158.859) (-151.660) -- 0:01:00 685000 -- [-150.861] (-154.427) (-154.565) (-155.432) * (-157.162) [-149.219] (-152.359) (-148.461) -- 0:01:00 Average standard deviation of split frequencies: 0.006264 686000 -- [-141.726] (-149.986) (-151.427) (-166.321) * (-162.369) (-140.778) (-153.060) [-141.083] -- 0:01:00 687000 -- [-146.359] (-155.241) (-147.722) (-168.001) * (-155.838) (-148.215) (-157.369) [-147.727] -- 0:01:00 688000 -- [-146.361] (-151.994) (-147.409) (-161.423) * (-163.930) [-140.347] (-154.287) (-146.208) -- 0:00:59 689000 -- [-144.541] (-149.135) (-145.358) (-164.298) * (-159.395) [-140.321] (-165.320) (-144.145) -- 0:00:59 690000 -- (-144.779) (-148.338) [-146.278] (-167.876) * (-154.812) [-144.274] (-161.552) (-154.799) -- 0:00:59 Average standard deviation of split frequencies: 0.006416 691000 -- [-141.606] (-147.987) (-149.217) (-161.013) * (-159.839) [-145.498] (-152.799) (-145.952) -- 0:00:59 692000 -- (-148.171) (-151.230) [-147.973] (-161.546) * (-162.995) [-145.528] (-153.027) (-146.270) -- 0:00:59 693000 -- [-147.635] (-150.956) (-150.815) (-156.341) * (-158.848) [-143.287] (-159.147) (-152.910) -- 0:00:58 694000 -- (-150.993) (-146.256) [-149.562] (-168.184) * (-160.950) [-144.530] (-162.528) (-148.607) -- 0:00:58 695000 -- [-148.636] (-146.163) (-153.393) (-163.485) * (-162.254) (-148.180) (-167.780) [-140.724] -- 0:00:58 Average standard deviation of split frequencies: 0.006123 696000 -- [-143.950] (-149.492) (-144.098) (-160.046) * (-159.811) [-145.733] (-173.801) (-150.602) -- 0:00:58 697000 -- (-150.020) [-143.191] (-150.180) (-161.964) * (-157.426) (-148.257) (-154.736) [-143.816] -- 0:00:58 698000 -- (-163.444) (-152.230) [-147.249] (-162.283) * (-158.560) [-143.787] (-149.478) (-147.226) -- 0:00:57 699000 -- (-155.900) (-146.162) [-145.078] (-163.116) * (-153.364) [-151.745] (-150.069) (-140.044) -- 0:00:57 700000 -- (-158.328) (-148.137) [-143.788] (-160.264) * (-168.360) [-146.542] (-150.864) (-146.247) -- 0:00:57 Average standard deviation of split frequencies: 0.006217 701000 -- (-163.451) (-154.805) [-143.335] (-169.151) * (-167.260) [-138.692] (-151.406) (-147.998) -- 0:00:57 702000 -- (-170.104) (-145.681) [-141.434] (-159.023) * (-162.542) [-150.897] (-140.758) (-152.003) -- 0:00:57 703000 -- (-156.423) [-148.014] (-144.318) (-162.542) * (-154.840) (-146.058) (-159.470) [-143.482] -- 0:00:57 704000 -- (-161.023) [-140.789] (-146.112) (-156.844) * (-160.635) [-142.069] (-157.145) (-149.070) -- 0:00:56 705000 -- (-154.826) [-147.391] (-148.912) (-167.417) * (-157.584) (-142.510) (-162.544) [-144.778] -- 0:00:56 Average standard deviation of split frequencies: 0.006090 706000 -- (-163.394) (-150.217) [-147.307] (-154.800) * (-163.893) [-141.320] (-163.043) (-152.390) -- 0:00:56 707000 -- (-165.815) [-141.457] (-147.970) (-164.191) * [-144.685] (-145.046) (-168.084) (-146.700) -- 0:00:56 708000 -- (-156.669) [-147.029] (-142.604) (-174.126) * [-141.155] (-144.691) (-171.879) (-146.733) -- 0:00:56 709000 -- (-155.692) (-153.210) [-144.113] (-164.413) * (-147.341) (-155.852) (-163.462) [-148.644] -- 0:00:55 710000 -- [-149.227] (-145.913) (-145.461) (-162.329) * [-142.635] (-145.941) (-158.360) (-146.286) -- 0:00:55 Average standard deviation of split frequencies: 0.005970 711000 -- (-148.021) (-145.639) [-147.057] (-155.005) * (-146.162) [-141.311] (-155.707) (-156.045) -- 0:00:55 712000 -- [-140.351] (-145.077) (-148.276) (-161.979) * (-144.886) [-139.157] (-159.414) (-145.044) -- 0:00:55 713000 -- (-148.257) [-140.854] (-151.197) (-164.496) * [-148.489] (-142.718) (-167.213) (-143.953) -- 0:00:55 714000 -- [-144.517] (-150.961) (-158.383) (-162.986) * (-146.461) (-166.711) (-164.700) [-144.499] -- 0:00:54 715000 -- [-147.832] (-147.191) (-152.488) (-163.416) * [-145.349] (-157.764) (-161.807) (-150.758) -- 0:00:54 Average standard deviation of split frequencies: 0.005706 716000 -- (-159.596) [-146.174] (-151.657) (-152.927) * (-152.426) (-145.460) (-166.291) [-143.918] -- 0:00:54 717000 -- (-165.484) [-147.823] (-154.024) (-162.806) * (-148.949) (-159.356) (-168.514) [-147.000] -- 0:00:54 718000 -- (-162.657) [-146.964] (-142.550) (-159.940) * [-147.422] (-145.270) (-157.388) (-149.974) -- 0:00:54 719000 -- (-159.783) [-153.186] (-145.675) (-160.324) * (-154.122) (-162.343) (-160.215) [-144.045] -- 0:00:53 720000 -- (-165.046) [-147.697] (-144.563) (-154.035) * [-141.289] (-151.754) (-164.317) (-148.865) -- 0:00:53 Average standard deviation of split frequencies: 0.005972 721000 -- (-156.361) [-148.801] (-152.374) (-154.589) * [-140.251] (-148.964) (-162.983) (-146.621) -- 0:00:53 722000 -- (-150.569) [-151.502] (-149.180) (-158.870) * (-157.046) [-144.178] (-155.315) (-151.033) -- 0:00:53 723000 -- (-158.536) [-138.654] (-148.522) (-162.524) * [-149.994] (-148.451) (-155.522) (-149.242) -- 0:00:53 724000 -- (-158.819) [-148.469] (-144.780) (-158.920) * [-143.941] (-149.509) (-158.537) (-154.118) -- 0:00:52 725000 -- (-159.148) (-156.086) [-143.883] (-159.094) * (-147.484) (-150.647) (-157.069) [-145.851] -- 0:00:52 Average standard deviation of split frequencies: 0.005817 726000 -- (-160.589) [-143.628] (-147.402) (-156.464) * [-140.956] (-156.964) (-161.802) (-151.539) -- 0:00:52 727000 -- (-152.037) [-141.206] (-146.572) (-163.022) * (-151.272) (-153.980) (-160.195) [-147.764] -- 0:00:52 728000 -- (-159.521) [-148.809] (-150.704) (-173.175) * [-145.902] (-152.581) (-157.897) (-141.316) -- 0:00:52 729000 -- (-167.957) [-145.764] (-147.094) (-160.982) * [-146.542] (-154.145) (-161.034) (-148.954) -- 0:00:52 730000 -- (-162.404) (-153.728) [-142.553] (-150.280) * (-147.559) [-142.943] (-164.970) (-153.663) -- 0:00:51 Average standard deviation of split frequencies: 0.005403 731000 -- (-157.792) (-150.857) [-145.436] (-154.395) * (-143.501) [-148.142] (-159.059) (-149.653) -- 0:00:51 732000 -- (-167.911) (-144.042) [-148.334] (-154.446) * [-145.732] (-144.319) (-162.802) (-148.252) -- 0:00:51 733000 -- (-162.086) (-147.692) [-147.729] (-162.252) * [-140.435] (-147.303) (-155.993) (-152.978) -- 0:00:51 734000 -- (-159.660) [-141.627] (-151.633) (-160.958) * (-141.364) [-143.870] (-161.857) (-159.182) -- 0:00:51 735000 -- (-157.649) [-138.812] (-146.981) (-158.439) * (-143.612) [-147.058] (-164.302) (-159.024) -- 0:00:50 Average standard deviation of split frequencies: 0.005151 736000 -- (-164.177) (-150.699) [-152.993] (-162.396) * (-150.663) [-143.384] (-158.792) (-159.557) -- 0:00:50 737000 -- (-148.625) (-144.563) [-147.814] (-155.888) * (-143.167) [-142.783] (-156.975) (-171.922) -- 0:00:50 738000 -- (-150.066) (-144.990) [-144.287] (-164.828) * [-152.305] (-149.161) (-160.367) (-155.906) -- 0:00:50 739000 -- [-140.884] (-146.885) (-159.851) (-163.400) * [-150.213] (-153.263) (-163.379) (-156.827) -- 0:00:50 740000 -- [-145.157] (-147.594) (-158.130) (-153.022) * (-144.034) (-152.765) (-166.701) [-142.171] -- 0:00:49 Average standard deviation of split frequencies: 0.004800 741000 -- [-144.003] (-146.187) (-157.138) (-158.165) * [-156.418] (-150.958) (-167.857) (-162.610) -- 0:00:49 742000 -- (-150.360) [-146.578] (-162.841) (-165.827) * (-147.092) [-150.299] (-161.216) (-147.852) -- 0:00:49 743000 -- (-143.624) [-147.971] (-154.321) (-167.226) * [-145.255] (-146.968) (-162.480) (-153.452) -- 0:00:49 744000 -- (-154.988) [-145.025] (-160.154) (-163.354) * (-151.792) [-147.037] (-159.565) (-151.670) -- 0:00:49 745000 -- (-150.843) [-143.544] (-161.724) (-167.043) * (-150.160) [-144.305] (-162.528) (-157.796) -- 0:00:48 Average standard deviation of split frequencies: 0.004845 746000 -- (-142.032) (-146.959) [-141.822] (-162.307) * (-147.230) [-147.419] (-159.765) (-160.558) -- 0:00:48 747000 -- (-146.586) [-144.610] (-152.032) (-156.148) * [-146.515] (-154.285) (-166.219) (-155.027) -- 0:00:48 748000 -- [-142.607] (-153.587) (-140.721) (-154.915) * (-147.438) [-149.813] (-161.370) (-169.943) -- 0:00:48 749000 -- (-147.150) [-142.440] (-162.274) (-147.572) * [-141.289] (-153.070) (-156.688) (-152.934) -- 0:00:48 750000 -- (-145.093) [-145.056] (-160.962) (-145.351) * (-145.036) [-143.884] (-160.498) (-163.278) -- 0:00:48 Average standard deviation of split frequencies: 0.004945 751000 -- (-159.732) [-139.185] (-158.118) (-148.214) * (-148.111) [-154.862] (-148.905) (-162.451) -- 0:00:47 752000 -- (-150.682) [-147.255] (-159.284) (-149.988) * [-145.238] (-157.771) (-160.045) (-153.245) -- 0:00:47 753000 -- (-156.367) (-157.365) (-162.328) [-148.538] * [-144.561] (-146.250) (-167.674) (-158.781) -- 0:00:47 754000 -- (-147.316) [-142.404] (-154.278) (-140.836) * [-145.672] (-152.674) (-165.743) (-157.582) -- 0:00:47 755000 -- (-175.420) [-147.447] (-157.577) (-147.852) * [-143.533] (-149.192) (-168.750) (-158.300) -- 0:00:47 Average standard deviation of split frequencies: 0.005118 756000 -- [-137.235] (-148.214) (-164.138) (-152.166) * [-140.680] (-151.930) (-166.248) (-160.775) -- 0:00:46 757000 -- (-150.649) [-145.257] (-163.161) (-148.452) * [-141.845] (-150.353) (-163.214) (-155.772) -- 0:00:46 758000 -- (-145.136) [-146.765] (-165.610) (-145.929) * [-145.147] (-160.509) (-166.060) (-156.026) -- 0:00:46 759000 -- (-144.315) (-148.845) (-169.967) [-145.173] * [-141.568] (-143.082) (-166.648) (-148.445) -- 0:00:46 760000 -- (-153.697) (-144.064) (-160.694) [-147.538] * (-147.401) [-143.653] (-158.732) (-159.644) -- 0:00:46 Average standard deviation of split frequencies: 0.005526 761000 -- (-157.675) (-157.721) [-149.360] (-154.053) * [-142.210] (-157.415) (-164.050) (-154.967) -- 0:00:45 762000 -- (-165.917) [-146.696] (-154.071) (-143.064) * [-143.772] (-153.014) (-159.363) (-143.553) -- 0:00:45 763000 -- (-158.719) (-146.067) [-150.931] (-150.085) * [-143.958] (-148.704) (-165.645) (-152.217) -- 0:00:45 764000 -- (-164.866) [-138.883] (-157.576) (-157.758) * [-147.918] (-140.370) (-163.623) (-155.646) -- 0:00:45 765000 -- [-143.918] (-144.129) (-142.908) (-156.294) * [-155.035] (-144.985) (-174.618) (-162.474) -- 0:00:45 Average standard deviation of split frequencies: 0.005923 766000 -- (-149.455) [-144.257] (-147.523) (-161.785) * [-146.787] (-148.822) (-156.163) (-154.461) -- 0:00:44 767000 -- [-144.340] (-148.046) (-146.848) (-157.289) * (-145.597) [-150.262] (-171.577) (-164.532) -- 0:00:44 768000 -- (-145.247) [-144.915] (-148.419) (-158.819) * [-144.491] (-143.258) (-164.106) (-158.976) -- 0:00:44 769000 -- (-147.844) (-141.803) [-146.556] (-162.463) * (-145.729) (-146.704) [-149.805] (-158.353) -- 0:00:44 770000 -- (-152.797) [-137.350] (-153.362) (-163.082) * [-155.245] (-147.806) (-146.477) (-168.540) -- 0:00:44 Average standard deviation of split frequencies: 0.006295 771000 -- (-164.208) [-142.866] (-144.590) (-167.687) * [-147.503] (-143.159) (-144.388) (-162.441) -- 0:00:43 772000 -- (-158.714) [-154.654] (-153.210) (-170.015) * [-153.274] (-151.561) (-155.936) (-163.435) -- 0:00:43 773000 -- (-163.009) (-147.011) [-144.491] (-168.191) * (-144.528) (-146.304) [-146.110] (-158.992) -- 0:00:43 774000 -- (-150.126) (-154.626) [-147.490] (-163.535) * (-145.892) (-145.552) [-146.041] (-167.218) -- 0:00:43 775000 -- [-140.931] (-162.375) (-144.217) (-165.357) * (-145.306) [-143.968] (-158.269) (-159.075) -- 0:00:43 Average standard deviation of split frequencies: 0.006201 776000 -- (-147.855) (-149.233) [-142.103] (-158.441) * [-142.788] (-143.452) (-144.324) (-154.485) -- 0:00:43 777000 -- (-144.243) [-146.218] (-149.394) (-169.295) * (-146.503) (-147.404) [-153.536] (-156.157) -- 0:00:42 778000 -- (-158.544) [-150.797] (-143.934) (-160.714) * [-148.104] (-150.530) (-152.281) (-155.737) -- 0:00:42 779000 -- [-147.259] (-144.507) (-144.362) (-159.507) * (-154.321) [-150.694] (-157.603) (-162.132) -- 0:00:42 780000 -- (-147.088) (-163.346) [-154.273] (-160.468) * (-152.069) [-139.522] (-154.019) (-161.011) -- 0:00:42 Average standard deviation of split frequencies: 0.005862 781000 -- (-150.581) (-158.640) [-147.548] (-159.140) * [-145.156] (-152.810) (-157.905) (-160.366) -- 0:00:42 782000 -- (-139.960) (-162.270) [-142.370] (-160.027) * [-156.003] (-139.752) (-165.163) (-160.644) -- 0:00:41 783000 -- (-141.558) (-161.288) (-149.499) [-145.644] * (-154.247) [-141.931] (-151.496) (-154.637) -- 0:00:41 784000 -- [-139.618] (-161.623) (-149.965) (-154.519) * (-150.448) [-141.694] (-150.655) (-156.048) -- 0:00:41 785000 -- [-145.838] (-166.788) (-144.599) (-155.244) * (-148.185) [-143.070] (-152.770) (-163.648) -- 0:00:41 Average standard deviation of split frequencies: 0.005823 786000 -- [-140.424] (-170.925) (-145.095) (-144.006) * (-152.581) (-138.670) (-148.535) [-143.060] -- 0:00:41 787000 -- [-146.050] (-160.323) (-168.512) (-148.355) * (-150.503) (-154.470) (-147.932) [-146.308] -- 0:00:40 788000 -- [-143.857] (-156.118) (-166.854) (-146.073) * (-157.099) (-158.318) [-143.472] (-142.759) -- 0:00:40 789000 -- (-144.250) (-162.091) (-166.371) [-139.188] * [-151.005] (-162.857) (-146.594) (-145.163) -- 0:00:40 790000 -- [-151.090] (-151.112) (-155.812) (-142.129) * [-142.079] (-170.678) (-145.396) (-155.358) -- 0:00:40 Average standard deviation of split frequencies: 0.005795 791000 -- (-142.859) (-165.925) (-158.536) [-140.234] * (-146.774) (-169.453) [-147.201] (-151.149) -- 0:00:40 792000 -- [-147.318] (-156.989) (-161.665) (-146.722) * [-144.922] (-164.890) (-151.547) (-156.846) -- 0:00:39 793000 -- (-142.443) (-157.861) (-147.715) [-144.511] * (-170.763) (-163.001) [-147.003] (-146.017) -- 0:00:39 794000 -- [-148.524] (-164.953) (-146.584) (-152.151) * (-158.574) (-163.751) (-146.588) [-144.357] -- 0:00:39 795000 -- [-143.051] (-171.332) (-148.036) (-139.943) * (-160.220) (-163.692) (-145.404) [-143.513] -- 0:00:39 Average standard deviation of split frequencies: 0.005827 796000 -- (-146.229) (-163.734) [-146.607] (-147.493) * [-144.976] (-166.214) (-155.940) (-150.842) -- 0:00:39 797000 -- (-148.041) (-167.458) [-147.969] (-147.548) * (-149.345) (-165.935) (-150.886) [-140.090] -- 0:00:38 798000 -- (-142.907) (-162.816) [-145.223] (-158.486) * (-161.720) (-162.690) (-151.702) [-145.999] -- 0:00:38 799000 -- [-148.205] (-161.560) (-145.644) (-160.539) * (-148.311) (-155.374) (-161.386) [-146.670] -- 0:00:38 800000 -- [-149.879] (-157.748) (-148.865) (-156.999) * [-139.444] (-156.366) (-150.967) (-148.764) -- 0:00:38 Average standard deviation of split frequencies: 0.005841 801000 -- (-147.229) (-157.065) [-143.037] (-168.485) * [-148.445] (-163.982) (-152.637) (-147.154) -- 0:00:38 802000 -- [-150.669] (-160.838) (-150.593) (-161.767) * (-146.336) (-157.914) (-166.864) [-143.089] -- 0:00:38 803000 -- (-153.743) (-160.560) [-144.375] (-174.078) * (-150.372) (-156.467) (-158.816) [-144.530] -- 0:00:37 804000 -- (-144.513) (-149.746) [-150.196] (-157.477) * (-146.891) (-166.015) (-166.806) [-148.960] -- 0:00:37 805000 -- (-153.028) (-166.273) [-142.549] (-154.173) * (-146.146) (-153.765) (-160.191) [-141.884] -- 0:00:37 Average standard deviation of split frequencies: 0.005871 806000 -- [-144.079] (-161.940) (-143.726) (-158.535) * [-141.345] (-153.044) (-164.175) (-143.161) -- 0:00:37 807000 -- [-150.851] (-161.909) (-159.682) (-161.797) * [-148.544] (-159.238) (-161.388) (-144.685) -- 0:00:37 808000 -- (-150.861) (-164.312) [-140.626] (-161.220) * [-138.173] (-150.243) (-164.318) (-143.866) -- 0:00:36 809000 -- (-151.999) (-156.339) [-147.459] (-146.786) * [-145.563] (-159.428) (-161.026) (-148.668) -- 0:00:36 810000 -- [-151.942] (-154.227) (-147.187) (-160.015) * (-155.724) (-147.720) (-164.589) [-142.137] -- 0:00:36 Average standard deviation of split frequencies: 0.006048 811000 -- [-145.617] (-151.139) (-145.868) (-156.404) * (-167.950) (-153.198) (-160.694) [-145.871] -- 0:00:36 812000 -- [-146.813] (-157.051) (-142.257) (-162.132) * (-154.218) (-152.706) (-162.588) [-145.413] -- 0:00:36 813000 -- (-149.467) [-145.318] (-137.495) (-157.338) * (-153.653) [-148.797] (-159.089) (-148.375) -- 0:00:35 814000 -- [-144.781] (-147.044) (-147.467) (-158.757) * (-159.301) (-142.880) (-154.708) [-148.532] -- 0:00:35 815000 -- [-142.560] (-146.455) (-146.072) (-159.263) * (-152.799) [-147.114] (-157.904) (-149.962) -- 0:00:35 Average standard deviation of split frequencies: 0.005777 816000 -- (-145.863) [-146.455] (-150.736) (-160.959) * (-148.073) (-149.575) (-155.462) [-142.514] -- 0:00:35 817000 -- (-150.024) (-163.867) [-141.063] (-162.479) * [-147.929] (-145.386) (-156.241) (-146.036) -- 0:00:35 818000 -- (-140.453) (-170.609) [-152.400] (-158.106) * (-152.431) [-141.878] (-157.719) (-150.364) -- 0:00:34 819000 -- [-144.999] (-168.591) (-151.610) (-159.209) * (-146.524) (-156.507) (-148.273) [-153.277] -- 0:00:34 820000 -- (-144.009) (-164.817) [-151.170] (-164.177) * (-140.346) (-162.497) (-158.975) [-144.082] -- 0:00:34 Average standard deviation of split frequencies: 0.005899 821000 -- (-150.437) (-167.425) [-142.389] (-166.176) * (-142.471) (-159.050) (-163.681) [-139.960] -- 0:00:34 822000 -- [-145.252] (-161.519) (-147.102) (-148.251) * (-145.165) (-144.396) (-157.425) [-145.044] -- 0:00:34 823000 -- (-154.066) (-166.183) (-150.831) [-152.970] * [-149.994] (-151.309) (-163.830) (-143.531) -- 0:00:33 824000 -- (-148.671) (-162.953) [-140.126] (-151.835) * [-146.665] (-147.255) (-155.307) (-159.208) -- 0:00:33 825000 -- (-142.184) (-157.994) [-150.100] (-142.278) * [-143.104] (-152.589) (-161.407) (-150.828) -- 0:00:33 Average standard deviation of split frequencies: 0.005817 826000 -- [-151.169] (-158.851) (-143.786) (-149.178) * [-146.601] (-149.729) (-163.245) (-154.674) -- 0:00:33 827000 -- [-145.916] (-153.440) (-160.776) (-148.288) * (-150.432) (-156.672) (-163.734) [-147.979] -- 0:00:33 828000 -- [-144.097] (-170.851) (-152.996) (-143.724) * (-153.778) [-146.157] (-168.875) (-156.391) -- 0:00:33 829000 -- (-147.753) (-167.274) (-157.868) [-149.502] * (-142.627) (-160.057) (-161.949) [-154.812] -- 0:00:32 830000 -- (-145.481) (-157.906) (-151.314) [-144.462] * [-146.694] (-163.350) (-159.806) (-161.360) -- 0:00:32 Average standard deviation of split frequencies: 0.005607 831000 -- (-148.089) (-170.146) [-152.083] (-149.662) * [-146.924] (-161.504) (-154.280) (-147.815) -- 0:00:32 832000 -- [-140.367] (-167.650) (-146.468) (-164.007) * [-143.561] (-166.491) (-154.543) (-147.199) -- 0:00:32 833000 -- [-143.939] (-168.308) (-153.127) (-159.850) * [-146.977] (-159.366) (-155.871) (-148.511) -- 0:00:32 834000 -- [-148.181] (-165.460) (-158.660) (-160.735) * [-149.605] (-158.340) (-157.875) (-147.597) -- 0:00:31 835000 -- [-147.312] (-165.535) (-159.109) (-152.556) * [-151.526] (-156.075) (-154.023) (-151.832) -- 0:00:31 Average standard deviation of split frequencies: 0.005530 836000 -- (-154.446) [-149.685] (-150.789) (-168.063) * [-146.034] (-157.840) (-151.277) (-143.819) -- 0:00:31 837000 -- (-150.569) [-143.709] (-158.791) (-164.496) * [-143.985] (-157.797) (-153.115) (-146.823) -- 0:00:31 838000 -- (-168.776) [-142.306] (-143.698) (-156.262) * [-157.508] (-175.294) (-157.706) (-146.577) -- 0:00:31 839000 -- (-146.554) [-145.218] (-144.393) (-161.537) * [-148.689] (-165.942) (-156.704) (-143.970) -- 0:00:30 840000 -- (-150.429) [-148.357] (-150.440) (-161.147) * (-142.482) (-168.817) (-162.614) [-146.458] -- 0:00:30 Average standard deviation of split frequencies: 0.005742 841000 -- (-147.799) (-143.006) [-155.020] (-154.667) * [-142.526] (-149.800) (-161.684) (-139.173) -- 0:00:30 842000 -- [-141.785] (-149.220) (-149.977) (-153.228) * (-147.579) (-153.664) (-165.677) [-150.871] -- 0:00:30 843000 -- (-147.031) [-155.457] (-148.489) (-159.839) * (-151.179) [-148.603] (-162.377) (-148.930) -- 0:00:30 844000 -- (-151.126) [-153.554] (-153.191) (-151.546) * (-153.655) (-142.528) [-144.418] (-140.626) -- 0:00:29 845000 -- (-144.353) (-147.241) [-152.016] (-163.022) * [-143.743] (-150.501) (-162.749) (-143.931) -- 0:00:29 Average standard deviation of split frequencies: 0.005929 846000 -- (-143.145) [-148.040] (-148.190) (-164.776) * [-146.313] (-156.166) (-141.791) (-167.627) -- 0:00:29 847000 -- (-142.427) [-149.259] (-153.718) (-155.819) * [-141.346] (-156.419) (-146.275) (-161.521) -- 0:00:29 848000 -- (-148.379) [-145.077] (-152.234) (-169.232) * (-149.731) (-151.586) [-152.454] (-156.680) -- 0:00:29 849000 -- (-143.430) [-149.983] (-165.504) (-160.994) * (-148.148) (-152.068) [-145.987] (-160.691) -- 0:00:28 850000 -- (-167.057) (-146.020) [-143.755] (-166.813) * (-148.793) [-142.640] (-142.783) (-162.472) -- 0:00:28 Average standard deviation of split frequencies: 0.005763 851000 -- (-156.044) (-142.729) [-142.046] (-170.124) * (-155.725) (-143.139) [-146.161] (-155.965) -- 0:00:28 852000 -- (-166.544) [-142.636] (-140.128) (-159.990) * (-150.696) (-147.402) [-152.636] (-162.368) -- 0:00:28 853000 -- (-158.649) [-146.242] (-146.819) (-143.452) * (-156.204) (-145.332) [-143.268] (-159.380) -- 0:00:28 854000 -- (-162.224) (-148.599) [-145.539] (-152.656) * (-151.706) (-142.294) [-142.606] (-156.733) -- 0:00:28 855000 -- (-157.371) [-156.783] (-145.435) (-158.496) * (-155.523) [-150.854] (-150.940) (-160.968) -- 0:00:27 Average standard deviation of split frequencies: 0.005551 856000 -- (-167.386) (-146.241) [-144.501] (-153.553) * (-161.685) [-146.698] (-146.687) (-161.733) -- 0:00:27 857000 -- (-164.876) [-139.879] (-147.812) (-148.637) * [-142.210] (-149.582) (-148.379) (-157.137) -- 0:00:27 858000 -- (-160.990) (-142.783) (-154.678) [-141.971] * (-151.990) (-151.896) [-145.546] (-170.663) -- 0:00:27 859000 -- (-157.101) [-146.199] (-156.373) (-143.904) * [-149.511] (-145.526) (-149.863) (-161.274) -- 0:00:27 860000 -- (-159.932) (-157.539) [-152.114] (-145.949) * [-146.232] (-156.210) (-153.658) (-156.567) -- 0:00:26 Average standard deviation of split frequencies: 0.005258 861000 -- (-161.383) (-146.247) (-142.880) [-146.077] * (-146.730) (-145.956) [-150.526] (-162.435) -- 0:00:26 862000 -- (-164.884) (-142.942) [-147.717] (-167.226) * [-145.601] (-143.140) (-145.792) (-155.480) -- 0:00:26 863000 -- (-165.886) [-147.799] (-145.342) (-159.542) * (-142.591) (-164.688) [-146.738] (-156.431) -- 0:00:26 864000 -- (-161.176) [-143.942] (-152.754) (-166.586) * (-146.377) (-161.941) [-147.759] (-157.230) -- 0:00:26 865000 -- (-154.693) [-146.125] (-152.160) (-156.880) * (-144.017) (-160.267) [-145.915] (-161.185) -- 0:00:25 Average standard deviation of split frequencies: 0.005509 866000 -- (-147.744) [-148.388] (-152.124) (-158.875) * [-149.236] (-160.771) (-154.000) (-158.195) -- 0:00:25 867000 -- [-145.095] (-144.997) (-154.627) (-157.308) * [-145.493] (-157.790) (-145.860) (-154.411) -- 0:00:25 868000 -- [-145.805] (-147.593) (-159.774) (-157.281) * [-146.847] (-154.391) (-153.998) (-162.960) -- 0:00:25 869000 -- (-146.609) [-145.010] (-155.767) (-156.339) * (-150.766) [-149.471] (-145.527) (-168.967) -- 0:00:25 870000 -- [-143.151] (-146.018) (-166.142) (-160.277) * (-155.934) [-140.826] (-147.019) (-160.101) -- 0:00:24 Average standard deviation of split frequencies: 0.005826 871000 -- (-147.235) [-140.293] (-164.060) (-153.475) * (-153.542) (-147.874) [-146.070] (-165.016) -- 0:00:24 872000 -- [-142.237] (-143.210) (-162.598) (-161.586) * (-149.891) [-144.292] (-153.619) (-164.590) -- 0:00:24 873000 -- (-145.420) [-147.708] (-163.610) (-153.572) * (-145.210) [-148.197] (-158.895) (-163.664) -- 0:00:24 874000 -- (-148.148) [-148.518] (-152.555) (-144.736) * (-152.250) [-144.389] (-160.224) (-159.186) -- 0:00:24 875000 -- (-148.785) [-145.712] (-166.076) (-155.210) * [-145.119] (-139.783) (-158.036) (-165.312) -- 0:00:24 Average standard deviation of split frequencies: 0.005963 876000 -- [-147.017] (-147.319) (-158.048) (-154.368) * (-151.876) [-149.072] (-155.469) (-162.036) -- 0:00:23 877000 -- (-149.130) [-151.851] (-156.861) (-160.080) * [-150.693] (-139.648) (-149.174) (-165.971) -- 0:00:23 878000 -- (-145.295) [-144.068] (-153.804) (-152.662) * (-158.529) (-149.782) [-144.973] (-164.634) -- 0:00:23 879000 -- [-144.944] (-148.199) (-162.035) (-150.243) * (-160.696) [-140.435] (-143.396) (-161.691) -- 0:00:23 880000 -- [-150.180] (-142.046) (-161.682) (-158.353) * [-141.067] (-148.216) (-147.325) (-169.326) -- 0:00:23 Average standard deviation of split frequencies: 0.005824 881000 -- (-141.321) [-143.499] (-164.774) (-157.073) * [-141.618] (-145.969) (-148.310) (-154.647) -- 0:00:22 882000 -- (-143.199) (-142.772) (-166.230) [-148.082] * [-141.956] (-159.186) (-150.741) (-163.964) -- 0:00:22 883000 -- (-147.268) (-148.857) (-157.060) [-147.303] * (-144.512) (-149.504) [-143.293] (-159.884) -- 0:00:22 884000 -- [-144.560] (-147.759) (-160.637) (-166.102) * (-146.811) [-140.676] (-143.559) (-162.482) -- 0:00:22 885000 -- [-147.883] (-144.776) (-156.519) (-149.946) * [-140.183] (-148.441) (-145.724) (-161.257) -- 0:00:22 Average standard deviation of split frequencies: 0.005853 886000 -- (-150.710) (-147.288) (-163.612) [-141.508] * [-139.540] (-141.376) (-169.599) (-170.636) -- 0:00:21 887000 -- (-156.089) [-143.550] (-160.743) (-148.091) * [-144.798] (-148.990) (-161.990) (-160.127) -- 0:00:21 888000 -- (-142.617) (-148.838) (-153.442) [-145.706] * [-145.642] (-142.615) (-163.597) (-158.928) -- 0:00:21 889000 -- (-148.821) [-145.694] (-167.337) (-158.755) * [-142.339] (-150.259) (-158.124) (-159.835) -- 0:00:21 890000 -- (-148.758) [-145.393] (-156.559) (-154.759) * (-150.491) [-149.383] (-161.126) (-156.786) -- 0:00:21 Average standard deviation of split frequencies: 0.005780 891000 -- [-147.602] (-150.864) (-159.994) (-163.289) * [-142.196] (-148.894) (-164.804) (-162.974) -- 0:00:20 892000 -- (-149.363) [-146.019] (-154.457) (-157.642) * [-149.135] (-148.935) (-155.546) (-166.245) -- 0:00:20 893000 -- (-142.379) [-140.593] (-165.823) (-164.800) * (-149.234) [-150.713] (-149.609) (-174.600) -- 0:00:20 894000 -- (-159.266) [-147.592] (-151.176) (-156.237) * (-151.962) [-142.813] (-149.897) (-168.639) -- 0:00:20 895000 -- (-165.652) (-144.066) [-144.744] (-161.688) * (-148.277) (-150.690) [-142.232] (-162.969) -- 0:00:20 Average standard deviation of split frequencies: 0.006061 896000 -- (-165.712) [-144.534] (-139.910) (-156.018) * [-142.499] (-147.002) (-152.270) (-169.630) -- 0:00:19 897000 -- (-162.527) [-146.755] (-142.259) (-166.342) * (-146.734) [-143.954] (-151.829) (-161.852) -- 0:00:19 898000 -- (-165.394) (-149.978) [-143.708] (-157.830) * (-148.611) [-146.719] (-143.708) (-166.367) -- 0:00:19 899000 -- (-158.994) (-148.717) (-147.626) [-154.729] * [-142.947] (-141.387) (-146.804) (-168.170) -- 0:00:19 900000 -- (-155.700) [-145.521] (-147.822) (-160.758) * [-147.148] (-146.038) (-151.382) (-157.456) -- 0:00:19 Average standard deviation of split frequencies: 0.006218 901000 -- (-171.321) (-143.228) [-146.892] (-152.355) * (-144.161) (-154.357) [-146.897] (-159.152) -- 0:00:19 902000 -- (-159.975) (-145.007) [-150.913] (-144.527) * (-151.823) (-161.298) [-145.846] (-169.361) -- 0:00:18 903000 -- (-161.284) (-144.489) [-146.658] (-165.918) * (-152.722) (-160.892) [-137.946] (-154.250) -- 0:00:18 904000 -- (-157.648) [-145.168] (-149.449) (-157.976) * (-152.530) (-159.292) [-147.647] (-159.533) -- 0:00:18 905000 -- (-163.641) (-144.298) (-154.376) [-147.613] * (-155.038) (-165.266) (-145.251) [-153.199] -- 0:00:18 Average standard deviation of split frequencies: 0.006222 906000 -- (-169.848) (-147.356) (-150.076) [-146.847] * (-145.306) (-159.245) (-154.668) [-145.361] -- 0:00:18 907000 -- (-159.818) [-142.634] (-159.147) (-152.309) * (-152.026) (-156.580) [-145.084] (-145.054) -- 0:00:17 908000 -- (-161.077) [-141.412] (-159.209) (-150.263) * (-150.492) (-159.049) (-154.082) [-145.014] -- 0:00:17 909000 -- (-161.459) (-155.575) (-150.857) [-144.109] * [-150.482] (-166.787) (-158.253) (-150.467) -- 0:00:17 910000 -- (-175.660) [-139.524] (-162.140) (-148.088) * [-142.630] (-166.308) (-163.166) (-155.567) -- 0:00:17 Average standard deviation of split frequencies: 0.006212 911000 -- (-163.462) [-156.932] (-162.667) (-153.965) * [-144.016] (-146.371) (-162.660) (-146.317) -- 0:00:17 912000 -- (-145.186) (-153.872) (-158.061) [-146.426] * (-149.153) (-146.387) [-144.750] (-154.411) -- 0:00:16 913000 -- (-152.068) [-145.756] (-163.203) (-151.160) * [-143.551] (-152.898) (-149.324) (-160.480) -- 0:00:16 914000 -- (-148.788) [-140.625] (-156.638) (-147.353) * [-142.690] (-155.272) (-142.251) (-159.760) -- 0:00:16 915000 -- (-143.839) (-170.126) (-160.642) [-147.541] * (-154.222) (-165.229) [-141.267] (-145.375) -- 0:00:16 Average standard deviation of split frequencies: 0.006004 916000 -- [-142.773] (-149.771) (-148.807) (-170.968) * [-141.606] (-164.795) (-145.767) (-150.495) -- 0:00:16 917000 -- (-141.334) (-148.995) [-144.305] (-168.525) * [-144.027] (-157.197) (-148.054) (-147.470) -- 0:00:15 918000 -- (-142.427) (-152.424) [-147.427] (-163.626) * (-149.385) (-162.598) (-157.551) [-145.837] -- 0:00:15 919000 -- (-150.654) [-141.891] (-160.793) (-160.094) * (-146.250) (-167.664) (-150.517) [-146.212] -- 0:00:15 920000 -- [-143.521] (-155.859) (-142.114) (-158.849) * (-150.611) (-158.440) (-148.074) [-150.760] -- 0:00:15 Average standard deviation of split frequencies: 0.006080 921000 -- [-138.878] (-151.591) (-143.554) (-151.189) * (-146.377) (-165.987) [-145.770] (-157.969) -- 0:00:15 922000 -- [-143.753] (-149.006) (-150.384) (-156.270) * (-141.568) (-156.229) [-150.619] (-164.918) -- 0:00:14 923000 -- [-142.389] (-144.653) (-150.466) (-156.678) * [-146.542] (-156.527) (-149.518) (-160.878) -- 0:00:14 924000 -- [-148.334] (-153.033) (-150.770) (-159.969) * (-145.975) (-165.463) [-146.097] (-163.774) -- 0:00:14 925000 -- (-154.212) (-146.537) [-145.688] (-163.876) * [-148.043] (-157.953) (-150.534) (-164.246) -- 0:00:14 Average standard deviation of split frequencies: 0.006374 926000 -- (-167.415) [-142.606] (-144.026) (-157.769) * [-145.106] (-164.775) (-147.048) (-162.781) -- 0:00:14 927000 -- (-158.876) (-144.497) [-144.571] (-149.576) * [-140.672] (-148.855) (-153.592) (-166.551) -- 0:00:14 928000 -- (-173.356) [-144.015] (-144.105) (-154.520) * (-145.413) (-149.695) [-146.326] (-160.326) -- 0:00:13 929000 -- (-161.324) (-154.503) [-141.706] (-153.572) * [-144.199] (-146.775) (-146.035) (-162.274) -- 0:00:13 930000 -- (-165.002) (-154.948) [-147.444] (-147.494) * (-146.240) [-147.421] (-164.740) (-165.876) -- 0:00:13 Average standard deviation of split frequencies: 0.006281 931000 -- (-160.433) (-166.705) (-147.387) [-142.915] * [-144.014] (-144.866) (-174.315) (-160.533) -- 0:00:13 932000 -- (-158.242) (-168.074) [-144.034] (-155.817) * (-140.966) [-156.411] (-159.879) (-151.852) -- 0:00:13 933000 -- (-170.288) (-172.727) [-140.753] (-147.338) * (-146.413) [-150.383] (-159.756) (-159.595) -- 0:00:12 934000 -- (-163.597) [-150.666] (-164.019) (-144.937) * [-143.600] (-144.612) (-174.575) (-163.402) -- 0:00:12 935000 -- (-160.291) (-155.985) (-164.775) [-148.238] * (-144.632) [-142.658] (-163.485) (-158.589) -- 0:00:12 Average standard deviation of split frequencies: 0.006205 936000 -- (-158.558) (-147.267) (-160.871) [-139.684] * [-149.680] (-145.076) (-158.662) (-162.789) -- 0:00:12 937000 -- (-156.251) (-149.669) (-165.257) [-144.640] * (-143.375) [-143.973] (-168.957) (-162.013) -- 0:00:12 938000 -- (-159.133) (-152.753) (-156.886) [-146.288] * [-140.051] (-149.910) (-158.088) (-154.198) -- 0:00:11 939000 -- (-157.831) [-141.881] (-155.915) (-145.962) * [-144.108] (-154.050) (-162.631) (-153.483) -- 0:00:11 940000 -- (-162.271) [-146.593] (-149.694) (-150.697) * [-148.073] (-154.516) (-163.751) (-159.176) -- 0:00:11 Average standard deviation of split frequencies: 0.005974 941000 -- (-157.269) (-142.119) (-141.331) [-148.474] * (-150.115) [-146.825] (-161.214) (-164.344) -- 0:00:11 942000 -- (-155.865) (-146.123) (-149.376) [-144.343] * (-148.263) [-149.350] (-155.395) (-158.078) -- 0:00:11 943000 -- (-167.868) [-143.340] (-146.093) (-147.284) * (-145.645) (-146.077) [-144.396] (-160.686) -- 0:00:10 944000 -- (-161.827) (-147.558) (-150.131) [-145.293] * (-150.042) [-152.020] (-146.612) (-157.082) -- 0:00:10 945000 -- (-168.883) (-162.227) (-144.461) [-147.782] * (-143.654) [-150.716] (-149.680) (-156.390) -- 0:00:10 Average standard deviation of split frequencies: 0.005780 946000 -- (-161.303) (-161.731) [-147.873] (-153.489) * (-141.508) (-155.325) [-144.496] (-159.389) -- 0:00:10 947000 -- (-158.760) (-169.044) (-147.185) [-144.582] * (-154.276) (-152.579) [-138.740] (-162.844) -- 0:00:10 948000 -- (-163.277) (-163.306) [-144.780] (-153.399) * (-161.151) (-146.885) [-152.035] (-162.472) -- 0:00:09 949000 -- (-160.517) (-159.474) [-151.134] (-148.789) * (-156.367) [-143.190] (-151.655) (-165.529) -- 0:00:09 950000 -- (-159.828) (-150.368) [-149.607] (-146.463) * (-159.717) [-145.439] (-141.609) (-165.349) -- 0:00:09 Average standard deviation of split frequencies: 0.005990 951000 -- (-157.529) (-150.486) [-147.722] (-145.530) * (-154.267) (-153.580) [-141.717] (-164.348) -- 0:00:09 952000 -- (-166.078) (-150.516) (-143.790) [-143.512] * (-156.984) [-150.938] (-139.083) (-161.768) -- 0:00:09 953000 -- (-165.027) (-163.269) (-146.407) [-149.400] * (-164.529) [-144.221] (-153.302) (-145.676) -- 0:00:09 954000 -- [-149.015] (-160.650) (-146.379) (-148.723) * (-158.595) (-153.266) (-159.008) [-144.356] -- 0:00:08 955000 -- (-147.140) (-160.057) [-146.501] (-158.901) * (-156.769) (-144.804) [-140.589] (-148.196) -- 0:00:08 Average standard deviation of split frequencies: 0.005897 956000 -- (-145.300) (-167.191) (-151.090) [-145.376] * (-154.183) (-147.539) (-153.649) [-145.430] -- 0:00:08 957000 -- (-149.077) (-160.605) (-150.878) [-144.607] * (-169.634) (-151.737) (-162.453) [-149.046] -- 0:00:08 958000 -- (-149.439) (-161.920) (-147.795) [-144.560] * (-157.243) [-143.263] (-148.958) (-145.857) -- 0:00:08 959000 -- (-142.000) (-159.370) (-145.121) [-149.632] * (-159.327) (-145.200) (-151.823) [-144.970] -- 0:00:07 960000 -- (-162.488) (-156.869) [-146.589] (-151.515) * (-158.128) (-145.151) (-156.218) [-146.808] -- 0:00:07 Average standard deviation of split frequencies: 0.005929 961000 -- (-159.132) (-157.003) [-147.573] (-142.928) * (-166.181) (-161.174) [-147.288] (-150.313) -- 0:00:07 962000 -- (-158.560) (-161.312) (-149.865) [-144.283] * (-154.057) (-159.948) (-141.950) [-145.774] -- 0:00:07 963000 -- (-167.668) (-161.396) [-144.242] (-155.785) * (-159.261) (-162.114) (-146.398) [-145.482] -- 0:00:07 964000 -- (-159.729) (-160.313) [-151.188] (-153.585) * (-163.548) (-156.337) [-147.893] (-149.279) -- 0:00:06 965000 -- (-164.479) (-155.873) [-146.294] (-145.063) * (-164.758) (-159.955) (-147.328) [-141.858] -- 0:00:06 Average standard deviation of split frequencies: 0.006227 966000 -- (-166.885) (-153.487) [-143.172] (-148.880) * (-154.679) (-161.451) [-145.226] (-145.109) -- 0:00:06 967000 -- (-165.972) (-157.741) [-142.731] (-150.018) * (-160.487) (-160.871) (-141.855) [-143.801] -- 0:00:06 968000 -- (-161.918) (-151.686) [-140.022] (-146.378) * (-161.165) (-163.675) (-146.622) [-151.340] -- 0:00:06 969000 -- (-156.165) [-145.570] (-142.595) (-168.125) * (-143.349) (-157.440) (-144.460) [-147.336] -- 0:00:05 970000 -- (-155.827) (-146.574) [-144.358] (-162.486) * (-159.600) (-166.611) [-142.870] (-139.584) -- 0:00:05 Average standard deviation of split frequencies: 0.005949 971000 -- (-165.618) (-145.964) [-143.934] (-158.817) * (-161.732) (-157.463) [-142.379] (-149.706) -- 0:00:05 972000 -- (-165.133) [-145.551] (-145.971) (-160.249) * (-159.795) (-151.686) [-145.618] (-149.642) -- 0:00:05 973000 -- (-153.318) (-146.134) [-141.268] (-163.560) * (-167.111) (-159.288) (-145.747) [-147.533] -- 0:00:05 974000 -- (-160.030) [-144.670] (-146.710) (-145.705) * (-158.548) (-154.252) (-151.715) [-143.779] -- 0:00:04 975000 -- (-165.890) [-139.830] (-151.869) (-144.828) * (-163.071) (-155.935) [-145.220] (-145.595) -- 0:00:04 Average standard deviation of split frequencies: 0.005997 976000 -- (-158.918) (-149.550) [-148.774] (-148.584) * (-167.763) (-161.826) [-144.116] (-142.073) -- 0:00:04 977000 -- (-157.690) (-151.347) [-143.396] (-143.092) * (-158.394) (-160.807) [-153.631] (-148.485) -- 0:00:04 978000 -- (-161.215) (-152.065) (-147.772) [-145.944] * (-163.358) (-156.144) (-146.701) [-151.959] -- 0:00:04 979000 -- (-157.505) (-141.198) [-146.910] (-147.392) * (-160.354) (-155.563) [-141.776] (-143.003) -- 0:00:04 980000 -- (-157.617) (-142.084) [-150.890] (-150.818) * (-159.051) (-154.060) (-145.041) [-143.975] -- 0:00:03 Average standard deviation of split frequencies: 0.006570 981000 -- (-160.111) (-146.528) [-145.984] (-141.340) * (-161.984) (-158.614) (-148.209) [-149.588] -- 0:00:03 982000 -- (-152.449) (-149.510) [-142.588] (-159.599) * (-160.714) (-159.621) (-155.345) [-144.719] -- 0:00:03 983000 -- [-140.025] (-143.980) (-150.059) (-166.657) * (-168.138) (-157.507) [-145.388] (-148.588) -- 0:00:03 984000 -- [-142.660] (-148.776) (-145.279) (-161.196) * (-156.152) (-165.532) (-146.882) [-142.434] -- 0:00:03 985000 -- (-153.680) (-144.639) [-148.163] (-159.203) * (-158.113) (-165.546) (-148.096) [-151.808] -- 0:00:02 Average standard deviation of split frequencies: 0.005996 986000 -- [-144.831] (-146.023) (-153.970) (-160.878) * (-171.121) (-161.911) (-155.684) [-140.030] -- 0:00:02 987000 -- (-153.637) (-148.397) [-139.382] (-158.178) * (-161.589) (-157.589) [-148.531] (-144.523) -- 0:00:02 988000 -- (-148.713) [-149.079] (-146.976) (-164.040) * (-159.193) (-155.976) [-148.639] (-144.466) -- 0:00:02 989000 -- (-165.838) [-151.286] (-152.611) (-161.417) * (-158.446) (-162.317) (-147.462) [-147.257] -- 0:00:02 990000 -- (-145.456) [-155.907] (-146.115) (-156.752) * (-157.498) (-153.262) [-146.824] (-144.719) -- 0:00:01 Average standard deviation of split frequencies: 0.005988 991000 -- [-147.115] (-144.843) (-147.414) (-158.851) * (-159.905) (-161.712) (-142.738) [-151.150] -- 0:00:01 992000 -- (-146.110) [-146.504] (-143.592) (-158.081) * (-159.622) (-158.452) [-143.584] (-146.084) -- 0:00:01 993000 -- (-147.804) [-143.301] (-146.974) (-153.395) * (-157.381) (-155.828) [-146.109] (-152.142) -- 0:00:01 994000 -- (-145.878) [-145.098] (-152.178) (-158.014) * (-158.948) (-156.432) (-146.704) [-150.952] -- 0:00:01 995000 -- (-150.316) [-142.228] (-160.188) (-155.548) * (-163.354) (-162.412) [-148.265] (-154.404) -- 0:00:00 Average standard deviation of split frequencies: 0.005642 996000 -- [-146.061] (-140.757) (-171.647) (-148.661) * (-143.299) (-162.625) (-150.390) [-144.139] -- 0:00:00 997000 -- [-143.884] (-143.634) (-164.486) (-159.940) * [-139.235] (-166.617) (-143.863) (-147.977) -- 0:00:00 998000 -- (-143.350) [-142.394] (-162.054) (-159.673) * (-146.825) (-155.729) [-139.214] (-145.943) -- 0:00:00 999000 -- (-152.177) [-139.168] (-156.935) (-167.231) * [-146.324] (-165.572) (-149.442) (-148.066) -- 0:00:00 1000000 -- [-146.893] (-141.552) (-160.199) (-168.363) * (-150.421) (-160.872) [-147.924] (-147.850) -- 0:00:00 Average standard deviation of split frequencies: 0.005418 Analysis completed in 3 mins 12 seconds Analysis used 191.74 seconds of CPU time Likelihood of best state for "cold" chain of run 1 was -134.62 Likelihood of best state for "cold" chain of run 2 was -134.80 Acceptance rates for the moves in the "cold" chain of run 1: With prob. (last 100) chain accepted proposals by move 70.2 % ( 73 %) Dirichlet(Revmat{all}) 89.9 % ( 84 %) Slider(Revmat{all}) 66.4 % ( 52 %) Dirichlet(Pi{all}) 63.1 % ( 44 %) Slider(Pi{all}) 80.4 % ( 65 %) Multiplier(Alpha{1,2}) 72.1 % ( 48 %) Multiplier(Alpha{3}) 90.7 % ( 85 %) Slider(Pinvar{all}) 65.3 % ( 73 %) ExtSPR(Tau{all},V{all}) 47.7 % ( 54 %) ExtTBR(Tau{all},V{all}) 66.8 % ( 73 %) NNI(Tau{all},V{all}) 54.7 % ( 60 %) ParsSPR(Tau{all},V{all}) 27.6 % ( 19 %) Multiplier(V{all}) 78.5 % ( 80 %) Nodeslider(V{all}) 30.1 % ( 27 %) TLMultiplier(V{all}) Acceptance rates for the moves in the "cold" chain of run 2: With prob. (last 100) chain accepted proposals by move 69.2 % ( 56 %) Dirichlet(Revmat{all}) 89.5 % ( 85 %) Slider(Revmat{all}) 66.0 % ( 52 %) Dirichlet(Pi{all}) 64.6 % ( 51 %) Slider(Pi{all}) 80.3 % ( 59 %) Multiplier(Alpha{1,2}) 72.2 % ( 46 %) Multiplier(Alpha{3}) 90.4 % ( 78 %) Slider(Pinvar{all}) 65.5 % ( 59 %) ExtSPR(Tau{all},V{all}) 47.2 % ( 47 %) ExtTBR(Tau{all},V{all}) 66.5 % ( 68 %) NNI(Tau{all},V{all}) 54.6 % ( 63 %) ParsSPR(Tau{all},V{all}) 27.7 % ( 26 %) Multiplier(V{all}) 78.5 % ( 64 %) Nodeslider(V{all}) 29.4 % ( 19 %) TLMultiplier(V{all}) Chain swap information for run 1: 1 2 3 4 ---------------------------------- 1 | 0.56 0.12 0.00 2 | 166924 0.27 0.00 3 | 166482 166141 0.24 4 | 167103 167018 166332 Chain swap information for run 2: 1 2 3 4 ---------------------------------- 1 | 0.56 0.11 0.00 2 | 166744 0.26 0.00 3 | 166315 166256 0.26 4 | 167246 166326 167113 Upper diagonal: Proportion of successful state exchanges between chains Lower diagonal: Number of attempted state exchanges between chains Chain information: ID -- Heat ----------- 1 -- 1.00 (cold chain) 2 -- 0.91 3 -- 0.83 4 -- 0.77 Heat = 1 / (1 + T * (ID - 1)) (where T = 0.10 is the temperature and ID is the chain number) Setting burn-in to 2500 Summarizing parameters in files /data/mrbayes_input.nex.run1.p and /data/mrbayes_input.nex.run2.p Writing summary statistics to file /data/mrbayes_input.nex.pstat Using relative burnin ('relburnin=yes'), discarding the first 25 % of samples Below are rough plots of the generation (x-axis) versus the log probability of observing the data (y-axis). You can use these graphs to determine what the burn in for your analysis should be. When the log probability starts to plateau you may be at station- arity. Sample trees and parameters after the log probability plateaus. Of course, this is not a guarantee that you are at sta- tionarity. Also examine the convergence diagnostics provided by the 'sump' and 'sumt' commands for all the parameters in your model. Remember that the burn in is the number of samples to dis- card. There are a total of ngen / samplefreq samples taken during a MCMC analysis. Overlay plot for both runs: (1 = Run number 1; 2 = Run number 2; * = Both runs) +------------------------------------------------------------+ -143.49 | 1 | | | | 2 | | 1 1 12 1 2 22 2 1 1 | | 212 1 2 * 21 1 1 2 2 1 | | 1 2 21 1 1 1 2 1 | | 21 12 1 1 1 * 21 1 1 2 | | 2 11 1 12 2 12 12 * * 2 2 1 2 22| | 1 22 2 212 2 1 2 * 1 1122 1 1| |* 2 1 2 1 2 2 1 1 *2 2 | | 1 2 1 2 1 2 2 2 2 | | 2 1 | | 1 1 2 | | 2 | | 1 | +------+-----+-----+-----+-----+-----+-----+-----+-----+-----+ -148.30 ^ ^ 250000 1000000 Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -141.62 -152.28 2 -141.56 -153.49 -------------------------------------- TOTAL -141.59 -153.06 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.323575 0.021259 0.107421 0.589939 0.296665 1375.77 1381.18 1.000 r(A<->C){all} 0.066166 0.003853 0.000028 0.194451 0.047245 188.28 244.81 1.003 r(A<->G){all} 0.338544 0.017350 0.101712 0.591974 0.322034 105.11 168.11 1.004 r(A<->T){all} 0.072738 0.003729 0.000010 0.193576 0.056693 224.46 282.23 1.003 r(C<->G){all} 0.061944 0.003560 0.000018 0.178664 0.042650 290.91 309.42 1.014 r(C<->T){all} 0.359973 0.017740 0.134312 0.638675 0.349662 126.47 162.21 1.004 r(G<->T){all} 0.100636 0.005388 0.000021 0.240551 0.085494 239.27 254.95 1.000 pi(A){all} 0.242488 0.002709 0.139781 0.343405 0.239447 610.53 649.44 1.000 pi(C){all} 0.195156 0.002259 0.105366 0.288018 0.191419 862.92 869.46 1.004 pi(G){all} 0.280028 0.002826 0.178457 0.382263 0.279258 833.50 904.95 1.001 pi(T){all} 0.282328 0.002996 0.182563 0.391300 0.280182 689.44 754.14 1.001 alpha{1,2} 0.573269 0.537577 0.000160 2.048770 0.309765 749.26 844.65 1.000 alpha{3} 1.486115 1.301605 0.000760 3.739900 1.214929 1041.44 1081.27 1.000 pinvar{all} 0.326316 0.037725 0.000123 0.659712 0.312234 662.30 674.51 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple Setting urn-in to 2500 Summarizing trees in files "/data/mrbayes_input.nex.run1.t" and "/data/mrbayes_input.nex.run2.t" Using relative burnin ('relburnin=yes'), discarding the first 25 % of sampled trees Writing statistics to files /data/mrbayes_input.nex.<parts|tstat|vstat|trprobs|con> Examining first file ... Found one tree block in file "/data/mrbayes_input.nex.run1.t" with 2001 trees in last block Expecting the same number of trees in the last tree block of all files Tree reading status: 0 10 20 30 40 50 60 70 80 90 100 v-------v-------v-------v-------v-------v-------v-------v-------v-------v-------v ********************************************************************************* Read a total of 4002 trees in 2 files (sampling 3002 of them) (Each file contained 2001 trees of which 1501 were sampled) General explanation: In an unrooted tree, a taxon bipartition (split) is specified by removing a branch, thereby dividing the species into those to the left and those to the right of the branch. Here, taxa to one side of the removed branch are denoted '.' and those to the other side are denoted '*'. Specifically, the '.' symbol is used for the taxa on the same side as the outgroup. In a rooted or clock tree, the tree is rooted using the model and not by reference to an outgroup. Each bipartition therefore corresponds to a clade, that is, a group that includes all the descendants of a particular branch in the tree. Taxa that are included in each clade are denoted using '*', and taxa that are not included are denoted using the '.' symbol. The output first includes a key to all the bipartitions with frequency larger or equual to (Minpartfreq) in at least one run. Minpartfreq is a parameter to sumt command and currently it is set to 0.10. This is followed by a table with statistics for the informative bipartitions (those including at least two taxa), sorted from highest to lowest probability. For each bipartition, the table gives the number of times the partition or split was observed in all runs (#obs) and the posterior probability of the bipartition (Probab.), which is the same as the split frequency. If several runs are summarized, this is followed by the minimum split frequency (Min(s)), the maximum frequency (Max(s)), and the standard deviation of frequencies (Stddev(s)) across runs. The latter value should approach 0 for all bipartitions as MCMC runs converge. This is followed by a table summarizing branch lengths, node heights (if a clock model was used) and relaxed clock parameters (if a relaxed clock model was used). The mean, variance, and 95 % credible interval are given for each of these parameters. If several runs are summarized, the potential scale reduction factor (PSRF) is also given; it should approach 1 as runs converge. Node heights will take calibration points into account, if such points were used in the analysis. Note that Stddev may be unreliable if the partition is not present in all runs (the last column indicates the number of runs that sampled the partition if more than one run is summarized). The PSRF is not calculated at all if the partition is not present in all runs.The PSRF is also sensitive to small sample sizes and it should only be considered a rough guide to convergence since some of the assumptions allowing one to interpret it as a true potential scale reduction factor are violated in MrBayes. List of taxa in bipartitions: 1 -- C1 2 -- C10 3 -- C11 4 -- C2 5 -- C3 6 -- C4 7 -- C5 8 -- C6 9 -- C7 10 -- C8 11 -- C9 Key to taxon bipartitions (saved to file "/data/mrbayes_input.nex.parts"): ID -- Partition ----------------- 1 -- .********** 2 -- .*......... 3 -- ..*........ 4 -- ...*....... 5 -- ....*...... 6 -- .....*..... 7 -- ......*.... 8 -- .......*... 9 -- ........*.. 10 -- .........*. 11 -- ..........* 12 -- .**.*....** 13 -- .**.*...*** 14 -- .**......** 15 -- .**.*.*.*** 16 -- ..*.......* 17 -- .**........ 18 -- .*.......*. 19 -- .........** 20 -- ..*......*. 21 -- .**.......* 22 -- .*........* 23 -- ..*......** 24 -- .**.*.*..** 25 -- .**.******* 26 -- ...*...*... 27 -- .**......*. 28 -- .****.***** 29 -- .....*.*... 30 -- .*.......** 31 -- .******.*** 32 -- ...*.*..... 33 -- .****.*.*** 34 -- .**.*.***** 35 -- .....**.... ----------------- Summary statistics for informative taxon bipartitions (saved to file "/data/mrbayes_input.nex.tstat"): ID #obs Probab. Sd(s)+ Min(s) Max(s) Nruns ---------------------------------------------------------------- 12 2820 0.939374 0.007537 0.934044 0.944704 2 13 2197 0.731845 0.011777 0.723518 0.740173 2 14 2036 0.678215 0.007537 0.672885 0.683544 2 15 782 0.260493 0.001884 0.259161 0.261825 2 16 568 0.189207 0.002827 0.187209 0.191206 2 17 565 0.188208 0.004240 0.185210 0.191206 2 18 536 0.178548 0.005653 0.174550 0.182545 2 19 531 0.176882 0.007066 0.171885 0.181879 2 20 522 0.173884 0.000942 0.173218 0.174550 2 21 514 0.171219 0.005653 0.167222 0.175217 2 22 513 0.170886 0.007066 0.165889 0.175883 2 23 508 0.169221 0.008480 0.163225 0.175217 2 24 483 0.160893 0.012719 0.151899 0.169887 2 25 469 0.156229 0.003298 0.153897 0.158561 2 26 455 0.151566 0.001413 0.150566 0.152565 2 27 448 0.149234 0.008480 0.143238 0.155230 2 28 441 0.146902 0.006124 0.142572 0.151233 2 29 438 0.145903 0.000942 0.145237 0.146569 2 30 428 0.142572 0.008480 0.136576 0.148568 2 31 425 0.141572 0.002355 0.139907 0.143238 2 32 424 0.141239 0.000942 0.140573 0.141905 2 33 311 0.103598 0.005182 0.099933 0.107262 2 34 306 0.101932 0.003769 0.099267 0.104597 2 35 300 0.099933 0.005653 0.095936 0.103931 2 ---------------------------------------------------------------- + Convergence diagnostic (standard deviation of split frequencies) should approach 0.0 as runs converge. Summary statistics for branch and node parameters (saved to file "/data/mrbayes_input.nex.vstat"): 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median PSRF+ Nruns ------------------------------------------------------------------------------------------- length{all}[1] 0.009733 0.000122 0.000004 0.030745 0.006226 1.000 2 length{all}[2] 0.020100 0.000314 0.000110 0.052544 0.015395 1.000 2 length{all}[3] 0.009890 0.000141 0.000002 0.030415 0.006385 1.000 2 length{all}[4] 0.009572 0.000114 0.000003 0.029331 0.006394 1.000 2 length{all}[5] 0.013329 0.000212 0.000004 0.042394 0.008618 1.000 2 length{all}[6] 0.009682 0.000120 0.000002 0.030354 0.006262 1.000 2 length{all}[7] 0.016440 0.000251 0.000011 0.045442 0.012274 1.000 2 length{all}[8] 0.009789 0.000122 0.000006 0.031414 0.006344 1.000 2 length{all}[9] 0.059555 0.001557 0.005512 0.136325 0.050736 1.001 2 length{all}[10] 0.010012 0.000140 0.000004 0.032256 0.006226 1.000 2 length{all}[11] 0.009919 0.000135 0.000008 0.030795 0.006431 1.001 2 length{all}[12] 0.046664 0.001302 0.000156 0.111365 0.037766 1.000 2 length{all}[13] 0.039894 0.001156 0.000255 0.097756 0.032366 1.000 2 length{all}[14] 0.019601 0.000448 0.000071 0.050865 0.014920 1.000 2 length{all}[15] 0.015690 0.000267 0.000060 0.046973 0.010934 1.002 2 length{all}[16] 0.009426 0.000123 0.000003 0.030580 0.005917 0.998 2 length{all}[17] 0.009893 0.000109 0.000002 0.029949 0.006826 0.998 2 length{all}[18] 0.009894 0.000148 0.000013 0.026513 0.006925 1.000 2 length{all}[19] 0.009478 0.000124 0.000009 0.031483 0.005828 1.000 2 length{all}[20] 0.009773 0.000139 0.000005 0.031257 0.006101 0.999 2 length{all}[21] 0.009293 0.000097 0.000029 0.024832 0.006436 0.998 2 length{all}[22] 0.010517 0.000146 0.000026 0.038045 0.007043 0.999 2 length{all}[23] 0.010416 0.000132 0.000046 0.032941 0.007007 0.998 2 length{all}[24] 0.018208 0.000311 0.000521 0.050078 0.012913 0.998 2 length{all}[25] 0.009135 0.000086 0.000003 0.027657 0.005936 1.006 2 length{all}[26] 0.009755 0.000115 0.000002 0.027791 0.006772 0.999 2 length{all}[27] 0.010626 0.000112 0.000034 0.032452 0.007447 1.002 2 length{all}[28] 0.008805 0.000088 0.000000 0.027384 0.005464 1.002 2 length{all}[29] 0.008744 0.000088 0.000048 0.026568 0.005831 0.998 2 length{all}[30] 0.009843 0.000122 0.000003 0.031835 0.005981 0.999 2 length{all}[31] 0.009223 0.000110 0.000028 0.030218 0.005933 1.000 2 length{all}[32] 0.009598 0.000108 0.000086 0.031592 0.006397 0.998 2 length{all}[33] 0.008682 0.000079 0.000008 0.025495 0.005938 1.000 2 length{all}[34] 0.009449 0.000087 0.000027 0.028571 0.006484 0.997 2 length{all}[35] 0.009973 0.000115 0.000011 0.030263 0.006598 0.997 2 ------------------------------------------------------------------------------------------- + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. NA is reported when deviation of parameter values within all runs is 0 or when a parameter value (a branch length, for instance) is not sampled in all runs. Summary statistics for partitions with frequency >= 0.10 in at least one run: Average standard deviation of split frequencies = 0.005418 Maximum standard deviation of split frequencies = 0.012719 Average PSRF for parameter values (excluding NA and >10.0) = 1.000 Maximum PSRF for parameter values = 1.006 Clade credibility values: /----------------------------------------------------------------------- C1 (1) | |----------------------------------------------------------------------- C2 (4) | |----------------------------------------------------------------------- C4 (6) | |----------------------------------------------------------------------- C5 (7) | |----------------------------------------------------------------------- C6 (8) + | /------------------ C10 (2) | | | |------------------ C11 (3) | /-------68-------+ | | |------------------ C8 (10) | | | | /--------94-------+ \------------------ C9 (11) | | | \--------73-------+ \----------------------------------- C3 (5) | \----------------------------------------------------- C7 (9) Phylogram (based on average branch lengths): /---- C1 (1) | |----- C2 (4) | |---- C4 (6) | |--------- C5 (7) | |---- C6 (8) + | /----------- C10 (2) | | | |----- C11 (3) | /---------+ | | |----- C8 (10) | | | | /--------------------------+ \----- C9 (11) | | | \----------------------+ \------ C3 (5) | \------------------------------------ C7 (9) |-------------| 0.020 expected changes per site Calculating tree probabilities... Credible sets of trees (2403 trees sampled): 50 % credible set contains 902 trees 90 % credible set contains 2103 trees 95 % credible set contains 2253 trees 99 % credible set contains 2373 trees Exiting mrbayes block Reached end of file Tasks completed, exiting program because mode is noninteractive To return control to the command line after completion of file processing, set mode to interactive with 'mb -i <filename>' (i is for interactive) or use 'set mode=interactive' Running FUBAR... [2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,C4,C5,C6,(((C10,C11,C8,C9),C3),C7))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **11** sequences, **19** codons, and **1** partitions from `/data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/results/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/results/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/results/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -132.88, AIC-c = 311.44 (22 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.225 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 1.000 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results ---- ## FUBAR inferred no sites under subject to positive selection at posterior probability >= 0.9
CLUSTAL FORMAT for T-COFFEE Version_12.00.7fb08c2 [http://www.tcoffee.org] [MODE: ], CPU=0.06 sec, SCORE=1000, Nseq=11, Len=19 171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD 79_1146_ORF_1a_1b_VIPR_P_62836706_12380_12439_1_NA_USA_Unknown_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD Black_1_VIPR_P_161213709_12348_12404_1_NA_USA_Unknown_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD DF_2_NA_VIPR_P_87242672_12374_12433_1_NA_NA_Unknown_Alphacoronavirus_1 STTIDQSYLNECGVLVQLD FIPV_79_1146_NA_VIPR_ALG1_63098798_12150_12206_1_NA_NA_Cat_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD Felis_catus_NLD_UU88_2010_Pp1a_VIPR_ALG1_530803157_12195_12251_1_2010_08_17_Netherlands_Cat_Alphacoronavirus_1 GATMDQSYLNECGVLVQLD K378_1a_VIPR_ALG1_422313319_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD S378_1a_VIPR_ALG1_422313330_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD 171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_10 SFTVDQSYLNECGVLVQLD 171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_11 SSTVDQSYLNECGVLVQLD . *:***************
>171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_1 GGCACCACTATTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >79_1146_ORF_1a_1b_VIPR_P_62836706_12380_12439_1_NA_USA_Unknown_Alphacoronavirus_1 GGCACCACTATTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1 AGTTCTACTGTTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >Black_1_VIPR_P_161213709_12348_12404_1_NA_USA_Unknown_Alphacoronavirus_1 GGCACCACTATTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >DF_2_NA_VIPR_P_87242672_12374_12433_1_NA_NA_Unknown_Alphacoronavirus_1 AGCACCACTATTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >FIPV_79_1146_NA_VIPR_ALG1_63098798_12150_12206_1_NA_NA_Cat_Alphacoronavirus_1 GGCACCACTATTGATCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >Felis_catus_NLD_UU88_2010_Pp1a_VIPR_ALG1_530803157_12195_12251_1_2010_08_17_Netherlands_Cat_Alphacoronavirus_1 GGTGCTACCATGGACCAGAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >K378_1a_VIPR_ALG1_422313319_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 AGTTCTACTGTTGATCAAAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >S378_1a_VIPR_ALG1_422313330_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 AGTTCTACTGTTGATCAAAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >UNKNOWN_AX154950_NA_VIPR_P_14536504_12309_12368_1_NA_NA_Unknown_Alphacoronavirus_1 AGTTTTACTGTTGATCAAAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC >partially_attenuated_Miller_M60_NA_VIPR_P_110746810_12284_12343_1_1987_USA_Swine_Alphacoronavirus_1 AGTTCTACTGTTGATCAAAGTTATTTAAACGAGTGCGGGGTTCTAGTGCAGCTCGAC
>171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD >79_1146_ORF_1a_1b_VIPR_P_62836706_12380_12439_1_NA_USA_Unknown_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD >A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD >Black_1_VIPR_P_161213709_12348_12404_1_NA_USA_Unknown_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD >DF_2_NA_VIPR_P_87242672_12374_12433_1_NA_NA_Unknown_Alphacoronavirus_1 STTIDQSYLNECGVLVQLD >FIPV_79_1146_NA_VIPR_ALG1_63098798_12150_12206_1_NA_NA_Cat_Alphacoronavirus_1 GTTIDQSYLNECGVLVQLD >Felis_catus_NLD_UU88_2010_Pp1a_VIPR_ALG1_530803157_12195_12251_1_2010_08_17_Netherlands_Cat_Alphacoronavirus_1 GATMDQSYLNECGVLVQLD >K378_1a_VIPR_ALG1_422313319_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD >S378_1a_VIPR_ALG1_422313330_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD >UNKNOWN_AX154950_NA_VIPR_P_14536504_12309_12368_1_NA_NA_Unknown_Alphacoronavirus_1 SFTVDQSYLNECGVLVQLD >partially_attenuated_Miller_M60_NA_VIPR_P_110746810_12284_12343_1_1987_USA_Swine_Alphacoronavirus_1 SSTVDQSYLNECGVLVQLD
Reading sequence file /data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/fasta/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1 Found 11 sequences of length 57 Alignment looks like a valid DNA alignment. Estimated diversity is (pairwise deletion - ignoring missing/ambig): 7.1% Found 6 informative sites. Writing alignment of informative sites to: Phi.inf.sites Writing list of informative sites to: Phi.inf.list Calculating all pairwise incompatibilities... Done: 0.0%100.0% Using a window size of 80 with k as 8 Too few informative sites to use normal approximation. Try doing a permutation test or increasing alignment length Can also try decreasing windowsize.
#NEXUS [ID: 7555419412] begin taxa; dimensions ntax=11; taxlabels 171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_1 UNKNOWN_AX154950_NA_VIPR_P_14536504_12309_12368_1_NA_NA_Unknown_Alphacoronavirus_1 partially_attenuated_Miller_M60_NA_VIPR_P_110746810_12284_12343_1_1987_USA_Swine_Alphacoronavirus_1 79_1146_ORF_1a_1b_VIPR_P_62836706_12380_12439_1_NA_USA_Unknown_Alphacoronavirus_1 A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1 Black_1_VIPR_P_161213709_12348_12404_1_NA_USA_Unknown_Alphacoronavirus_1 DF_2_NA_VIPR_P_87242672_12374_12433_1_NA_NA_Unknown_Alphacoronavirus_1 FIPV_79_1146_NA_VIPR_ALG1_63098798_12150_12206_1_NA_NA_Cat_Alphacoronavirus_1 Felis_catus_NLD_UU88_2010_Pp1a_VIPR_ALG1_530803157_12195_12251_1_2010_08_17_Netherlands_Cat_Alphacoronavirus_1 K378_1a_VIPR_ALG1_422313319_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 S378_1a_VIPR_ALG1_422313330_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 ; end; begin trees; translate 1 171_1a_VIPR_ALG1_422313311_12355_12411_1_1971_Germany_Dog_Alphacoronavirus_1, 2 UNKNOWN_AX154950_NA_VIPR_P_14536504_12309_12368_1_NA_NA_Unknown_Alphacoronavirus_1, 3 partially_attenuated_Miller_M60_NA_VIPR_P_110746810_12284_12343_1_1987_USA_Swine_Alphacoronavirus_1, 4 79_1146_ORF_1a_1b_VIPR_P_62836706_12380_12439_1_NA_USA_Unknown_Alphacoronavirus_1, 5 A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1, 6 Black_1_VIPR_P_161213709_12348_12404_1_NA_USA_Unknown_Alphacoronavirus_1, 7 DF_2_NA_VIPR_P_87242672_12374_12433_1_NA_NA_Unknown_Alphacoronavirus_1, 8 FIPV_79_1146_NA_VIPR_ALG1_63098798_12150_12206_1_NA_NA_Cat_Alphacoronavirus_1, 9 Felis_catus_NLD_UU88_2010_Pp1a_VIPR_ALG1_530803157_12195_12251_1_2010_08_17_Netherlands_Cat_Alphacoronavirus_1, 10 K378_1a_VIPR_ALG1_422313319_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1, 11 S378_1a_VIPR_ALG1_422313330_12264_12320_1_1978_USA_Dog_Alphacoronavirus_1 ; [Note: This tree contains information on the topology, branch lengths (if present), and the probability of the partition indicated by the branch.] tree con_50_majrule = (1:6.226442e-03,4:6.393667e-03,6:6.261856e-03,7:1.227361e-02,8:6.343534e-03,(((2:1.539478e-02,3:6.385401e-03,10:6.225724e-03,11:6.431155e-03)0.678:1.491957e-02,5:8.617870e-03)0.939:3.776619e-02,9:5.073591e-02)0.732:3.236592e-02); [Note: This tree contains information only on the topology and branch lengths (median of the posterior probability density).] tree con_50_majrule = (1:6.226442e-03,4:6.393667e-03,6:6.261856e-03,7:1.227361e-02,8:6.343534e-03,(((2:1.539478e-02,3:6.385401e-03,10:6.225724e-03,11:6.431155e-03):1.491957e-02,5:8.617870e-03):3.776619e-02,9:5.073591e-02):3.236592e-02); end;
Estimated marginal likelihoods for runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /data/mrbayes_input.nex.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -141.73 -152.56 2 -141.72 -154.91 -------------------------------------- TOTAL -141.73 -154.31 -------------------------------------- Model parameter summaries over the runs sampled in files "/data/mrbayes_input.nex.run1.p" and "/data/mrbayes_input.nex.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/data/mrbayes_input.nex.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 0.324707 0.022122 0.110791 0.607763 0.297195 1271.40 1386.20 1.000 r(A<->C){all} 0.063787 0.003933 0.000012 0.192315 0.044259 284.09 289.43 1.005 r(A<->G){all} 0.342964 0.017076 0.093315 0.591816 0.339198 93.45 133.24 1.010 r(A<->T){all} 0.077452 0.004119 0.000008 0.208162 0.062552 204.26 224.26 1.000 r(C<->G){all} 0.064250 0.003871 0.000002 0.184231 0.045722 194.15 214.29 1.000 r(C<->T){all} 0.352910 0.015936 0.117457 0.594664 0.345420 113.65 139.43 1.015 r(G<->T){all} 0.098637 0.004746 0.000317 0.234816 0.083536 183.80 253.12 1.010 pi(A){all} 0.241663 0.002641 0.144656 0.346215 0.239310 669.24 749.83 1.000 pi(C){all} 0.196624 0.002295 0.103435 0.284129 0.194183 645.99 762.79 1.004 pi(G){all} 0.276434 0.002825 0.182206 0.386350 0.274207 872.33 893.15 1.001 pi(T){all} 0.285279 0.003089 0.180605 0.394718 0.283671 821.25 855.12 1.001 alpha{1,2} 0.582281 0.528540 0.000224 1.982054 0.327552 780.91 827.42 1.001 alpha{3} 1.509678 1.261131 0.001708 3.829231 1.216155 1136.70 1168.23 1.000 pinvar{all} 0.325199 0.038307 0.001402 0.665188 0.312099 593.74 659.56 1.002 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge.
[2J[H /HYPHY 2.3.14.20190214beta(MP) for Linux on x86_64\ ***************** TYPES OF STANDARD ANALYSES ***************** (1) Selection Analyses (2) Evolutionary Hypothesis Testing (3) Relative evolutionary rate inference (4) Coevolutionary analysis (5) Basic Analyses (6) Codon Selection Analyses (7) Compartmentalization (8) Data File Tools (9) Miscellaneous (10) Model Comparison (11) Kernel Analysis Tools (12) Molecular Clock (13) Phylogeny Reconstruction (14) Positive Selection (15) Recombination (16) Selection/Recombination (17) Relative Rate (18) Relative Ratio (19) Substitution Rates Please select type of analyses you want to list (or press ENTER to process custom batch file):[2J[H***************** FILES IN 'Selection Analyses' ***************** (1) [MEME] Test for episodic site-level selection using MEME (Mixed Effects Model of Evolution). (2) [FEL] Test for pervasive site-level selection using FEL (Fixed Effects Likelihood). (3) [SLAC] Test for pervasive site-level selection using SLAC (Single Likelihood Ancestor Counting). (4) [FUBAR] Test for pervasive site-level selection using FUBAR (Fast Unconstrained Bayesian AppRoximation for inferring selection). (5) [BUSTED] Test for episodic gene-wide selection using BUSTED (Branch-site Unrestricted Statistical Test of Episodic Diversification). (6) [aBSREL] Test for lineage-specific evolution using the branch-site method aBS-REL (Adaptive Branch-Site Random Effects Likelihood). (7) [RELAX] Test for relaxation of selection pressure along a specified set of test branches using RELAX (a random effects test of selection relaxation). Please select the analysis you would like to perform (or press ENTER to return to the list of analysis types): Analysis Description -------------------- Perform a Fast Unbiased AppRoximate Bayesian (FUBAR) analysis of a coding sequence alignment to determine whether some sites have been subject to pervasive purifying or diversifying selection. v2.1 introduces two more methods for estimating the posterior distribution of grid weights: collapsed Gibbs MCMC (faster) and 0-th order Variation Bayes approximation (fastest). Please note that a FUBAR analysis generates a cache and a results JSON file in the same directory as directory as the original alignment. HyPhy needs to have write privileges to this directory. For example if the original file is in /home/sergei/FUBAR/data/pol.nex then at the end of a FUBAR run, there will also exist FUBAR-generated files /home/sergei/FUBAR/data/pol.nex.FUBAR.json, /home/sergei/FUBAR/data/pol.nex.fubrar.cache. They also provide checkpointing so that a partially completed analysis can be restarted. - __Requirements__: in-frame codon alignment (possibly partitioned) and a phylogenetic tree (one per partition) - __Citation__: FUBAR: a fast, unconstrained bayesian approximation for inferring selection (2013), Mol Biol Evol. 30(5):1196-205 - __Written by__: Sergei L Kosakovsky Pond - __Contact Information__: spond@temple.edu - __Analysis Version__: 2.1 ####Choose Genetic Code 1. [**Universal**] Universal code. (Genebank transl_table=1). 2. [**Vertebrate mtDNA**] Vertebrate mitochondrial DNA code. (Genebank transl_table=2). 3. [**Yeast mtDNA**] Yeast mitochondrial DNA code. (Genebank transl_table=3). 4. [**Mold/Protozoan mtDNA**] Mold, Protozoan and Coelenterate mitochondrial DNA and the Mycloplasma/Spiroplasma code. (Genebank transl_table=4). 5. [**Invertebrate mtDNA**] Invertebrate mitochondrial DNA code. (Genebank transl_table=5). 6. [**Ciliate Nuclear**] Ciliate, Dasycladacean and Hexamita Nuclear code. (Genebank transl_table=6). 7. [**Echinoderm mtDNA**] Echinoderm mitochondrial DNA code. (Genebank transl_table=9). 8. [**Euplotid Nuclear**] Euplotid Nuclear code. (Genebank transl_table=10). 9. [**Alt. Yeast Nuclear**] Alternative Yeast Nuclear code. (Genebank transl_table=12). 10. [**Ascidian mtDNA**] Ascidian mitochondrial DNA code. (Genebank transl_table=13). 11. [**Flatworm mtDNA**] Flatworm mitochondrial DNA code. (Genebank transl_table=14). 12. [**Blepharisma Nuclear**] Blepharisma Nuclear code. (Genebank transl_table=15). 13. [**Chlorophycean mtDNA**] Chlorophycean Mitochondrial Code (transl_table=16). 14. [**Trematode mtDNA**] Trematode Mitochondrial Code (transl_table=21). 15. [**Scenedesmus obliquus mtDNA**] Scenedesmus obliquus mitochondrial Code (transl_table=22). 16. [**Thraustochytrium mtDNA**] Thraustochytrium Mitochondrial Code (transl_table=23). 17. [**Pterobranchia mtDNA**] Pterobranchia Mitochondrial Code (transl_table=24). 18. [**SR1 and Gracilibacteria**] Candidate Division SR1 and Gracilibacteria Code (transl_table=25). 19. [**Pachysolen Nuclear**] Pachysolen tannophilus Nuclear Code (transl_table=26). >Please choose an option (or press q to cancel selection): >Select a coding sequence alignment file (`/usr/local/lib/hyphy/TemplateBatchFiles/SelectionAnalyses/`) >A tree was found in the data file: `(C1,C2,C4,C5,C6,(((C10,C11,C8,C9),C3),C7))` >Would you like to use it (y/n)? >Loaded a multiple sequence alignment with **11** sequences, **19** codons, and **1** partitions from `/data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/results/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1.fna` > FUBAR will write cache and result files to _/data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/results/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1.fna.FUBAR.cache_ and _/data//pss_subsets/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result/original_alignment/fubar/results/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1/A76_1a_VIPR_ALG1_418470984_12268_12324_1_1976_USA_Dog_Alphacoronavirus_1.result.1.fna.FUBAR.json_, respectively > Number of grid points per dimension (total number is D^2) (permissible range = [5,50], default value = 20, integer): ####Posterior estimation method 1. [**Metropolis-Hastings**] Full Metropolis-Hastings MCMC algorithm (slowest, original 2013 paper implementation) 2. [**Collapsed Gibbs**] Collapsed Gibbs sampler (intermediate speed) 3. [**Variational Bayes**] 0-th order Variational Bayes approximations (fastest, recommended default) >Please choose an option (or press q to cancel selection):> The concentration parameter of the Dirichlet prior (permissible range = [0.001,1], default value = 0.5): ### Obtaining branch lengths and nucleotide substitution biases under the nucleotide GTR model * Log(L) = -132.88, AIC-c = 311.44 (22 estimated parameters) * Tree length (expected substitutions/site) for partition 1 : 0.225 ### Computing the phylogenetic likelihood function on the grid * Determining appropriate tree scaling based on the best score from a 20 x 20 rate grid * Best scaling achieved for * synonymous rate = 1.227 * non-synonymous rate = 1.000 * Computing conditional site likelihoods on a 20 x 20 rate grid ### Running an iterative zeroth order variational Bayes procedure to estimate the posterior mean of rate weights * Using the following settings * Dirichlet alpha : 0.5 ### Tabulating site-level results ---- ## FUBAR inferred no sites under subject to positive selection at posterior probability >= 0.9
Not all of the following information may be relevant for the case being handled, since this project may be part of a much larger auto-PSS-genome project where several methods of detection of positively selected sites have been used. As such the aligned.score_ascii file may have more sequences than the file effectively used to detect positively selected codons, since the content of this file reflects the content of the file used for the master alignment, from which a subsample may have been taken # ### General parameters ### # # The maximum number of sequences to use for the master file sequence_limit=90 # The random seed random_seed=3976763 # ### Alignment ### # # The alignment method: clustalw, muscle, kalign, t_coffee, or amap align_method=muscle # Minimum support value for amino acid positions in the alignment tcoffee_min_score=3 # ### MrBayes ### # # Number of iterations in MrBayes mrbayes_generations=1000000 # MrBayes burnin mrbayes_burnin=2500 # ### FUBAR ### # # The maximum number of sequences to be used by FUBAR. fubar_sequence_limit=90 # The number of FUBAR runs fubar_runs=1 # ### codeML ### # # The maximum number of sequences to be used by CodeML codeml_sequence_limit=30 # The number of CodeML runs codeml_runs=1 # The CodeML models to be run, one or more of: '1', '2', '7', and/or '8'. codeml_models=1 2 7 8 # ### OmegaMap ### # # The maximum number of sequences to use in OmegaMap omegamap_sequence_limit=90 # The number of OmegaMap runs omegamap_runs=1 # The number of OmegaMap iterations omegamap_iterations=2500