--- EXPERIMENT NOTES --- EXPERIMENT PROPERTIES #Fri Nov 11 17:04:09 WET 2016 codeml.models=0 1 2 3 7 8 mrbayes.mpich= mrbayes.ngen=1000000 tcoffee.alignMethod=CLUSTALW2 tcoffee.params= tcoffee.maxSeqs=0 codeml.bin=codeml mrbayes.tburnin=2500 codeml.dir= input.sequences= mrbayes.pburnin=2500 mrbayes.bin=mb_adops tcoffee.bin=t_coffee_ADOPS mrbayes.dir=/usr/bin/ tcoffee.dir= tcoffee.minScore=3 input.fasta=/opt/ADOPS/2/ABCB7-PA/input.fasta input.names= mrbayes.params= codeml.params= --- PSRF SUMMARY Estimated marginal likelihoods for runs sampled in files "/opt/ADOPS/2/ABCB7-PA/batch/allfiles/mrbayes/input.fasta.fasta.mrb.run1.p" and "/opt/ADOPS/2/ABCB7-PA/batch/allfiles/mrbayes/input.fasta.fasta.mrb.run2.p": (Use the harmonic mean for Bayes factor comparisons of models) (Values are saved to the file /opt/ADOPS/2/ABCB7-PA/batch/allfiles/mrbayes/input.fasta.fasta.mrb.lstat) Run Arithmetic mean Harmonic mean -------------------------------------- 1 -7973.10 -7986.89 2 -7973.05 -7988.71 -------------------------------------- TOTAL -7973.08 -7988.16 -------------------------------------- Model parameter summaries over the runs sampled in files "/opt/ADOPS/2/ABCB7-PA/batch/allfiles/mrbayes/input.fasta.fasta.mrb.run1.p" and "/opt/ADOPS/2/ABCB7-PA/batch/allfiles/mrbayes/input.fasta.fasta.mrb.run2.p": Summaries are based on a total of 3002 samples from 2 runs. Each run produced 2001 samples of which 1501 samples were included. Parameter summaries saved to file "/opt/ADOPS/2/ABCB7-PA/batch/allfiles/mrbayes/input.fasta.fasta.mrb.pstat". 95% HPD Interval -------------------- Parameter Mean Variance Lower Upper Median min ESS* avg ESS PSRF+ ------------------------------------------------------------------------------------------------------ TL{all} 1.409626 0.006486 1.251543 1.561081 1.408305 1272.09 1330.19 1.000 r(A<->C){all} 0.077086 0.000124 0.056399 0.099712 0.076838 1012.87 1109.67 1.000 r(A<->G){all} 0.227190 0.000383 0.188361 0.263023 0.226774 847.46 1024.54 1.000 r(A<->T){all} 0.137132 0.000362 0.098660 0.173385 0.136753 822.52 900.44 1.000 r(C<->G){all} 0.028982 0.000027 0.018645 0.038956 0.028720 1095.63 1143.76 1.001 r(C<->T){all} 0.461812 0.000658 0.411074 0.512743 0.461533 920.34 1002.94 1.000 r(G<->T){all} 0.067798 0.000110 0.047511 0.088103 0.067304 969.95 1044.32 1.000 pi(A){all} 0.220383 0.000070 0.203899 0.236401 0.220303 1097.16 1121.65 1.000 pi(C){all} 0.300917 0.000078 0.283410 0.317892 0.300846 1088.05 1103.46 1.000 pi(G){all} 0.289890 0.000084 0.272413 0.307317 0.289698 1170.42 1196.01 1.000 pi(T){all} 0.188810 0.000053 0.173914 0.201926 0.188720 983.15 1123.81 1.000 alpha{1,2} 0.121273 0.000061 0.106808 0.136932 0.120674 1308.02 1396.03 1.000 alpha{3} 4.293818 0.851835 2.642309 6.069091 4.181074 1388.79 1444.89 1.000 pinvar{all} 0.357515 0.000648 0.306166 0.406049 0.357983 1156.18 1202.27 1.000 ------------------------------------------------------------------------------------------------------ * Convergence diagnostic (ESS = Estimated Sample Size); min and avg values correspond to minimal and average ESS among runs. ESS value below 100 may indicate that the parameter is undersampled. + Convergence diagnostic (PSRF = Potential Scale Reduction Factor; Gelman and Rubin, 1992) should approach 1.0 as runs converge. Setting sumt conformat to Simple --- CODEML SUMMARY Model 1: NearlyNeutral -7461.223666 Model 2: PositiveSelection -7461.223666 Model 0: one-ratio -7529.150783 Model 3: discrete -7452.626183 Model 7: beta -7456.63158 Model 8: beta&w>1 -7453.226744 Model 0 vs 1 135.85423400000036 Model 2 vs 1 0.0 Model 8 vs 7 6.809672000001228 Additional information for M7 vs M8: Naive Empirical Bayes (NEB) analysis Bayes Empirical Bayes (BEB) analysis (Yang, Wong & Nielsen 2005. Mol. Biol. Evol. 22:1107-1118) Positively selected sites (*: P>95%; **: P>99%) (amino acids refer to 1st sequence: D_melanogaster_ABCB7-PA) Pr(w>1) post mean +- SE for w 31 T 0.686 1.207 +- 0.455 97 P 0.710 1.218 +- 0.468 502 S 0.721 1.241 +- 0.439 548 A 0.657 1.173 +- 0.476 693 R 0.591 1.060 +- 0.559 727 I 0.885 1.408 +- 0.281 732 A 0.861 1.379 +- 0.329
>C1 MAGLLHLTKQCSIHLPAHLGRAKCYTLAKGPGTHVQARVLYSSLTKVDDK NSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAAKTTTLQRQRP YRPQQQQSRLCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIWPKE DPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVLS AATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLD LAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGV KCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAVDSL INYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFSS ALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQA LLDMRAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVSFEYEPGKPI FRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDIS AVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARMADL HDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATS SLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRVGER GTHSELLRQNGLYARLWETQTQQFDPSREINEEVAAKKTRGVAooo >C2 MAGLLHLTKQCSIHLPAHLGRAKCYTLAKGPGTHVQARVLYSSLTKVDDK NSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAAKTTTLQRQRP YRPQQQQSRLCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIWPKE DPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVLS AATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLD LAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGV KCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAVDSL INYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFSS ALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQA LLDMQAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVNFEYEPGKPI FRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDIS AVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARMADL HDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATS SLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRVGER GTHSELLRQNGLYARLWETQTQQFDPSREINEEVEAKKTRGVAooo >C3 MAGLLHLTKQCSIHLPAHLGRAKCYTLAKGPGTHVQARVLYSSLTKVDDK NSNDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAAKTTTLQRQRP YRPQQQQSRLCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIWPKE DPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVLS AATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLD LAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGV KCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAVDSL INYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFSS ALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQA LLDMQAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVNFEYEPGKPI FRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDIS AVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARMADL HDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATS SLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRVGER GTHSELLRQNGLYARLWETQTQQFDPSREINEDVEAKKTRGVAooo >C4 MAGLLHLTKQCSIHLPAHLGRAKCYSLAKGPVTHVQARVLYSSLTKVDDQ NSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAAKTTTLQRQRL YRPQQQQSRLCHVHIGGGNDGGSRLGKLDAPEVTSKDMLRAMMAYIWPKE DPMVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVLS AATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLD LAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGV KCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAVDSL INYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFSS ALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQA LLDMQAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVSFEYEPGKPI FRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDIS TVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARMADL HDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATS SLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRVGER GTHSELLRQNGLYARLWETQTQQFDPSRGNKEEAAAKETRGVAooo >C5 MAGLLHLTKQCSIHLPGHLGRAKCYTLAKGTGVHVQARVLYSSLAKVDDQ SSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAATTTTLQRQRQ RLYRPQQQQSRQCHVHLGGGSDGGSGLGKLDAPEVTSQDMLRAMMAYIWP KEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAV LSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHN LDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSIL GVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAVD SLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIF SSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVR QALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVNFEYEPGK PIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQD ISGVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHEEVEKAARMA DLHDSIMSWPAQYATQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEA TSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGQLG ERGTHLELLKQNGLYARLWETQTQQFDPSREAKEEETPAKESRGVA >C6 MAGLLHLTKQCSIHLPAHLGRAKCYTFAKGTGTHVHARVLYSSLAKVNDQ GSSDVKHKKITPFTPTPGSKLLGGVFGKKGKGGAPAAAAAKTTTLQRQRR LYRPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIWPK EDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVL STATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNL DLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILG VKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAVDS LINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFS SALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQ ALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVSFEYEPGKP IFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLYRFFEPNSGKVLIGGQDI STVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHEEVENAARMAD LHDSIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEAT SSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGQLGE RGTHSELLRQNGLYARLWETQTQQFDPSRETKEEAPTKKSRGVAoo >C7 MAGLLHLTKQCSIHLPAHLGRAKCYTFAKRTGTHVQARVLYSSLAKVDDQ GSSDVKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAAKTTTLQRQRR LYRPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMMRAMMAYIWPK EDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVL STATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNL DLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILG VKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAVDS LINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFS SALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQ ALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVNFEYEPGKP IFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDI STVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHEEVENAARMAD LHDSIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEAT SSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGQLGE RGTHSELLRQNGLYARLWETQTQQFDPSREINEEGPTKKSRGVAoo >C8 MAGLLHLTKQCSIHLPAHLGRAKCYTFVKGTGKHVQARVLYSSLAKVDDQ GNSDVKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAASAKTTTLQRQRPY RPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIWPKED PLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAILST ATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLDL AFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGVK CGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAVDSLI NYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFSSA LSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQAL LDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVSFEYEPGKPIF RDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDISA VDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVENAARMADLH DSIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATSS LDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGHVGERG THSELLRQNGLYARLWETQTQQFDPTREIKEEAEAKKSRGAAoooo >C9 MAGLIYLTKQCSIHLPAHLGRAKCYTLAKGSHVQARVLFSSLAKVDDQGK NDVKRKKVTPFTPTPGSKLLGGVFGNKAKGGAPATAAAAKTTKLQRQRPY RPQQQQSRQCHVHIGGGSDGGPGLGKLDAPEVTSKDMLRAMMAYIWPKED PVVRKRVAISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVLSA ATAMMLGYGIARASASGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLDL AFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGIK CGLAFAGVSMGCVGIYAVYTLSVTQWRTQFRVYMNQAENEAGNKAVDSLI NYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAIFSSA LSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQAL LDMQAMFTLMNVDSSIQTASNAQPLFVDTSNSSIEFRNVSFEYEPGKPIF KDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQDISG VDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLTKSHAEVENAARMADLH DSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATSS LDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRVGEQG THSELLRKNGLYARLWETQTHQFDPSRDSKEEATAKASRGVAoooo >C10 MAGLLHLTKKCSIHLPAHLGRAKCYTFVKGTHVQARVLYSSLVKAEDQGN SDVKRKKITPFTPTPGSKLLGGVFGKKAKGGAPAAAAKTTKLQRQRLYRP QQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIWPKEDPL VRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDAVLSAAT ALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLHNLDLAF HLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSILGVKCG LAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAVDSLINY ETVKYFNNEKYEAGCYNEVLKKYETASLKTSSSLALLNFGQNAIFSSALS LIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREVRQALLD MQAMFTLMNVDSSIQTASNAQPLFVDTSNSSIEFRNVNFEYEPGKPIFRD LSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLVGGQDISAVD LESLRKVIAVVPQDSVLFHNTIEHNIHYGNLTKSHAEVENAARMADLHES IMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDEATSSLD SITEHNILQALTRATTGRTSICIAHRLSTVKDADEILVLENGRVGERGTH PELLRQNGLYARLWETQTQQFDPSRESKDEAAAKKARGVAoooooo CLUSTAL FORMAT for T-COFFEE Version_10.00.r1613 [http://www.tcoffee.org] [MODE: ], CPU=0.00 sec, SCORE=100, Nseq=10, Len=753 C1 MAGLLHLTKQCSIHLPAHLGRAKCYTLAKGPGTHVQARVLYSSLTKVDDK C2 MAGLLHLTKQCSIHLPAHLGRAKCYTLAKGPGTHVQARVLYSSLTKVDDK C3 MAGLLHLTKQCSIHLPAHLGRAKCYTLAKGPGTHVQARVLYSSLTKVDDK C4 MAGLLHLTKQCSIHLPAHLGRAKCYSLAKGPVTHVQARVLYSSLTKVDDQ C5 MAGLLHLTKQCSIHLPGHLGRAKCYTLAKGTGVHVQARVLYSSLAKVDDQ C6 MAGLLHLTKQCSIHLPAHLGRAKCYTFAKGTGTHVHARVLYSSLAKVNDQ C7 MAGLLHLTKQCSIHLPAHLGRAKCYTFAKRTGTHVQARVLYSSLAKVDDQ C8 MAGLLHLTKQCSIHLPAHLGRAKCYTFVKGTGKHVQARVLYSSLAKVDDQ C9 MAGLIYLTKQCSIHLPAHLGRAKCYTLAKG--SHVQARVLFSSLAKVDDQ C10 MAGLLHLTKKCSIHLPAHLGRAKCYTFVKG--THVQARVLYSSLVKAEDQ ****::***:******.********::.* **:****:***.*.:*: C1 NSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPA-AAAAKTTTLQRQR C2 NSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPA-AAAAKTTTLQRQR C3 NSNDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPA-AAAAKTTTLQRQR C4 NSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPA-AAAAKTTTLQRQR C5 SSSDAKRKKITPFTPTPGSKLLGGVFGKKAKGGAPA-AAAATTTTLQRQR C6 GSSDVKHKKITPFTPTPGSKLLGGVFGKKGKGGAPA-AAAAKTTTLQRQR C7 GSSDVKRKKITPFTPTPGSKLLGGVFGKKAKGGAPA-AAAAKTTTLQRQR C8 GNSDVKRKKITPFTPTPGSKLLGGVFGKKAKGGAP--AASAKTTTLQRQR C9 GKNDVKRKKVTPFTPTPGSKLLGGVFGNKAKGGAPATAAAAKTTKLQRQR C10 GNSDVKRKKITPFTPTPGSKLLGGVFGKKAKGGAP--AAAAKTTKLQRQR ...*.*:**:*****************:*.***** **:*.**.***** C1 --PYRPQQQQSRLCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIW C2 --PYRPQQQQSRLCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIW C3 --PYRPQQQQSRLCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIW C4 --LYRPQQQQSRLCHVHIGGGNDGGSRLGKLDAPEVTSKDMLRAMMAYIW C5 QRLYRPQQQQSRQCHVHLGGGSDGGSGLGKLDAPEVTSQDMLRAMMAYIW C6 -RLYRPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIW C7 -RLYRPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMMRAMMAYIW C8 --PYRPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIW C9 --PYRPQQQQSRQCHVHIGGGSDGGPGLGKLDAPEVTSKDMLRAMMAYIW C10 --LYRPQQQQSRQCHVHIGGGSDGGSGLGKLDAPEVTSKDMLRAMMAYIW ********* ****:***.***. ***********:**:******** C1 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C2 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C3 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C4 PKEDPMVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C5 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C6 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C7 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C8 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C9 PKEDPVVRKRVAISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA C10 PKEDPLVRKRVGISLGLLAGSKLLTVCVPFLFKGAVDTMTTLNMDTAPDA *****:*****.************************************** C1 VLSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C2 VLSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C3 VLSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C4 VLSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C5 VLSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C6 VLSTATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C7 VLSTATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C8 ILSTATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH C9 VLSAATAMMLGYGIARASASGFNELRNAVFAKVAHHSIRKIASNVFLHLH C10 VLSAATALMLGYGIARASAAGFNELRNAVFAKVAHHSIRKIASNVFLHLH :**:***:***********:****************************** C1 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C2 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C3 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C4 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C5 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C6 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C7 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C8 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C9 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI C10 NLDLAFHLNKQTGALSKTIDRGSRGINFVLSAMVFNIVPTIFELALVSSI ************************************************** C1 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAV C2 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAV C3 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAV C4 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAV C5 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAV C6 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVFMNQAENEAGNKAV C7 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAV C8 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAV C9 LGIKCGLAFAGVSMGCVGIYAVYTLSVTQWRTQFRVYMNQAENEAGNKAV C10 LGVKCGLAFAGVSMGCVGIYAAYTLSVTQWRTQFRVYMNQAENEAGNKAV **:******************.**************:************* C1 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C2 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C3 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C4 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C5 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C6 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C7 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C8 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C9 DSLINYETVKYFNNEKYEAGCYNEVLKKYEAASLKTSSSLALLNFGQNAI C10 DSLINYETVKYFNNEKYEAGCYNEVLKKYETASLKTSSSLALLNFGQNAI ******************************:******************* C1 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C2 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C3 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C4 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C5 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C6 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C7 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C8 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C9 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV C10 FSSALSLIMVLAAKEIAQGNMTVGDLVMVNALLFQLSIPLGFLGSVYREV ************************************************** C1 RQALLDMRAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVSFEYEPG C2 RQALLDMQAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVNFEYEPG C3 RQALLDMQAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVNFEYEPG C4 RQALLDMQAMFTLMNVDSSIQTAANAQPLFVDTTNSSIEFRNVSFEYEPG C5 RQALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVNFEYEPG C6 RQALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVSFEYEPG C7 RQALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVNFEYEPG C8 RQALLDMQAMFTLMNVDSRIQTAANAQPLFVDTTNSSIEFRNVSFEYEPG C9 RQALLDMQAMFTLMNVDSSIQTASNAQPLFVDTSNSSIEFRNVSFEYEPG C10 RQALLDMQAMFTLMNVDSSIQTASNAQPLFVDTSNSSIEFRNVNFEYEPG *******:********** ****:*********:*********.****** C1 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C2 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C3 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C4 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C5 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C6 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLYRFFEPNSGKVLIGGQ C7 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C8 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C9 KPIFKDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLIGGQ C10 KPIFRDLSFTIPAGKNVAIVGGSGSGKSSMVRLLFRFFEPNSGKVLVGGQ ****:*****************************:***********:*** C1 DISAVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARM C2 DISAVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARM C3 DISAVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARM C4 DISTVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVQNAARM C5 DISGVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHEEVEKAARM C6 DISTVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHEEVENAARM C7 DISTVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHEEVENAARM C8 DISAVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLSKSHAEVENAARM C9 DISGVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLTKSHAEVENAARM C10 DISAVDLESLRKVIAVVPQDSVLFHNTIEHNIHYGNLTKSHAEVENAARM *** *********************************:*** **::**** C1 ADLHDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C2 ADLHDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C3 ADLHDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C4 ADLHDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C5 ADLHDSIMSWPAQYATQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C6 ADLHDSIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C7 ADLHDSIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C8 ADLHDSIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C9 ADLHDSIMSWPGQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE C10 ADLHESIMSWPAQYSTQVGERGLKLSGGEKQRVAIARAILKNTPILIFDE ****:******.**:*********************************** C1 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRV C2 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRV C3 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRV C4 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRV C5 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGQL C6 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGQL C7 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGQL C8 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGHV C9 ATSSLDSITEHNILQALTRATSGRTSICIAHRLSTVKDADEILVLENGRV C10 ATSSLDSITEHNILQALTRATTGRTSICIAHRLSTVKDADEILVLENGRV *********************:**************************:: C1 GERGTHSELLRQNGLYARLWETQTQQFDPSREINEE-VAAKKTRGVAooo C2 GERGTHSELLRQNGLYARLWETQTQQFDPSREINEE-VEAKKTRGVAooo C3 GERGTHSELLRQNGLYARLWETQTQQFDPSREINED-VEAKKTRGVAooo C4 GERGTHSELLRQNGLYARLWETQTQQFDPSRGNKEE-AAAKETRGVAooo C5 GERGTHLELLKQNGLYARLWETQTQQFDPSREAKEEETPAKESRGVA--- C6 GERGTHSELLRQNGLYARLWETQTQQFDPSRETKEE-APTKKSRGVAoo- C7 GERGTHSELLRQNGLYARLWETQTQQFDPSREINEE-GPTKKSRGVAoo- C8 GERGTHSELLRQNGLYARLWETQTQQFDPTREIKEE-AEAKKSRGAAooo C9 GEQGTHSELLRKNGLYARLWETQTHQFDPSRDSKEE-ATAKASRGVAooo C10 GERGTHPELLRQNGLYARLWETQTQQFDPSRESKDE-AAAKKARGVAooo **:*** ***::************:****:* ::: :* :**.* C1 --- C2 --- C3 --- C4 --- C5 --- C6 --- C7 --- C8 o-- C9 o-- C10 ooo PROGRAM: T-COFFEE Version_10.00.r1613 (2013-10-22 15:49:04 - Revision 1613 - Build 427) -full_log S [0] -genepred_score S [0] nsd -run_name S [0] -mem_mode S [0] mem -extend D [1] 1 -extend_mode S [0] very_fast_triplet -max_n_pair D [0] 10 -seq_name_for_quadruplet S [0] all -compact S [0] default -clean S [0] no -do_self FL [0] 0 -do_normalise D [0] 1000 -template_file S [0] -setenv S [0] 0 -template_mode S [0] -flip D [0] 0 -remove_template_file D [0] 0 -profile_template_file S [0] -in S [0] -seq S [0] -aln S [0] -method_limits S [0] -method S [0] -lib S [0] -profile S [0] -profile1 S [0] -profile2 S [0] -pdb S [0] -relax_lib D [0] 1 -filter_lib D [0] 0 -shrink_lib D [0] 0 -out_lib W_F [0] no -out_lib_mode S [0] primary -lib_only D [0] 0 -outseqweight W_F [0] no -dpa FL [0] 0 -seq_source S [0] ANY -cosmetic_penalty D [0] 0 -gapopen D [0] 0 -gapext D [0] 0 -fgapopen D [0] 0 -fgapext D [0] 0 -nomatch D [0] 0 -newtree W_F [0] default -tree W_F [0] NO -usetree R_F [0] -tree_mode S [0] nj -distance_matrix_mode S [0] ktup -distance_matrix_sim_mode S [0] idmat_sim1 -quicktree FL [0] 0 -outfile W_F [0] default -maximise FL [1] 1 -output S [1] score_ascii html score_ascii -len D [0] 0 -infile R_F [1] /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln -matrix S [0] default -tg_mode D [0] 1 -profile_mode S [0] cw_profile_profile -profile_comparison S [0] profile -dp_mode S [0] linked_pair_wise -ktuple D [0] 1 -ndiag D [0] 0 -diag_threshold D [0] 0 -diag_mode D [0] 0 -sim_matrix S [0] vasiliky -transform S [0] -extend_seq FL [0] 0 -outorder S [0] input -inorder S [0] aligned -seqnos S [0] off -case S [0] keep -cpu D [0] 0 -maxnseq D [0] 1000 -maxlen D [0] -1 -sample_dp D [0] 0 -weight S [0] default -seq_weight S [0] no -align FL [1] 1 -mocca FL [0] 0 -domain FL [0] 0 -start D [0] 0 -len D [0] 0 -scale D [0] 0 -mocca_interactive FL [0] 0 -method_evaluate_mode S [0] default -evaluate_mode S [1] t_coffee_fast -get_type FL [0] 0 -clean_aln D [0] 0 -clean_threshold D [1] 1 -clean_iteration D [1] 1 -clean_evaluate_mode S [0] t_coffee_fast -extend_matrix FL [0] 0 -prot_min_sim D [40] 40 -prot_max_sim D [90] 90 -prot_min_cov D [40] 40 -pdb_type S [0] d -pdb_min_sim D [35] 35 -pdb_max_sim D [100] 100 -pdb_min_cov D [50] 50 -pdb_blast_server W_F [0] EBI -blast W_F [0] -blast_server W_F [0] EBI -pdb_db W_F [0] pdb -protein_db W_F [0] uniprot -method_log W_F [0] no -struc_to_use S [0] -cache W_F [0] use -align_pdb_param_file W_F [0] no -align_pdb_hasch_mode W_F [0] hasch_ca_trace_bubble -external_aligner S [0] NO -msa_mode S [0] tree -master S [0] no -blast_nseq D [0] 0 -lalign_n_top D [0] 10 -iterate D [1] 0 -trim D [0] 0 -split D [0] 0 -trimfile S [0] default -split D [0] 0 -split_nseq_thres D [0] 0 -split_score_thres D [0] 0 -check_pdb_status D [0] 0 -clean_seq_name D [0] 0 -seq_to_keep S [0] -dpa_master_aln S [0] -dpa_maxnseq D [0] 0 -dpa_min_score1 D [0] -dpa_min_score2 D [0] -dpa_keep_tmpfile FL [0] 0 -dpa_debug D [0] 0 -multi_core S [0] templates_jobs_relax_msa_evaluate -n_core D [0] 0 -max_n_proc D [0] 0 -lib_list S [0] -prune_lib_mode S [0] 5 -tip S [0] none -rna_lib S [0] -no_warning D [0] 0 -run_local_script D [0] 0 -plugins S [0] default -proxy S [0] unset -email S [0] -clean_overaln D [0] 0 -overaln_param S [0] -overaln_mode S [0] -overaln_model S [0] -overaln_threshold D [0] 0 -overaln_target D [0] 0 -overaln_P1 D [0] 0 -overaln_P2 D [0] 0 -overaln_P3 D [0] 0 -overaln_P4 D [0] 0 -exon_boundaries S [0] -dump S [0] no -display D [0] 100 INPUT FILES Input File (S) /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Format clustal_aln Input File (M) proba_pair Identify Master Sequences [no]: Master Sequences Identified INPUT SEQUENCES: 10 SEQUENCES [PROTEIN] Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C1 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C10 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C2 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C3 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C4 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C5 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C6 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C7 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C8 Length 746 type PROTEIN Struct Unchecked Input File /opt/ADOPS/2/ABCB7-PA/batch/allfiles/tcoffee/input.fasta.prot.fasta.clustalw2_rs_0_0.fasta.aln Seq C9 Length 746 type PROTEIN Struct Unchecked Multi Core Mode: 72 processors: ins S [0] default -proxy S [0] unset -email S [0] -clean_overaln D [0] 0 -overaln_param S [0] -overaln_mode S [0] -overaln_model S [0] -overaln_threshold D [0] 0 -overaln_target D [0] 0 -overaln_P1 D [0] 0 -overaln_P2 D [0] 0 -overaln_P3 D [0] 0 -overaln_P4 D [0] 0 -exon_boundaries S [0] -dump S [0] no -display D [0] 100 INPUT FILE